RLG00000023127

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0007246
Physical Location & Seq
Forward (+)
18552397 .. 18555296
2900 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023127

Sequence Viewer

Length: 1401 bp
ATGAGGAGGAGATCTTCTACTCTGCCTGTGGTGGATGTGTTTGTGGTACTTGTTGCATTGTTCACCGGTGCTCCGTTTCCATGGGCTGCTGTCAAGGGCAGTCATCCACATTGCAAGAAATTAAGGGAAAACTTGGGCGAAATTCGACCTCACAGTGTCACCATCACTGAATTTGGCGCAGTTGGTGATGGAGTCACTCTCAACACCAAGGCCTTTCAGAATGCCATCTTCTATCTCAACTCTTTTTCTGACAAGGGTGGGGCCAAGCTTTTTGTCCCCGCTGGCCGGTGGTTGACAGGAAGCTTCCATCTCATCAGTCATCTAACCTTGTGGTTGGATGAAGATGCAGTCATTCTTGGATCAACGAACTCAGATGATTGGCCAGTTGTTGATCCCTTGCCATCATATGGTCGAGGAAGGGAGTTGATGGGGGAAAGACATCAAAGCCTCATCTATGGGCACAATTTGACCGATGTTATCATAACTGGTCATAATGGAACGATTGATGGTCAAGGCAGCATTTGGTGGAATTGGTTCCGTAACAAAACTCTGAATTATACAAGGCCCCATTTGGTGGAGTTGATGAACTCAACTAGGGTTGTCATCTCAAACCTGACTTTTTTAAATTCACCATTTTGGACCATTCACCCAGTATACTGCAGCCATGTTTCCGTCCAGAATCTCACAATCCTTGCTCCTCTCAATTCACCAAACACGGATGGGATCGATCCAGACTCTTCTGATAATGTCTGCATTGAAGACTGTTATATCAGCACTGGTGATGATCTGATCTCCATCAAAAGTGGGTGGGATGAGTATGGCATTTCATATGGTCGTCCCAGTAGAAACATTCTCATTCGTGGGATTACAGGGAAAACTCAAACAAGTGCAGGAATTTCGATTGGAAGTGAGATGTCTGGAGGTGTTTCAGAAGTTCATGCAGAAAATCTCCAATTTTTTGGTTCTCATACAGGTATCAGAATAAAGACATCTCCAGGAAGGGGTGGTTATGTCAGAAATATCTGTGTGTCAAATATGACCTTGGATGGTGTATATATTGCTATAAGGTTCACCAGTCACTATGGAGAACACCCAGATGAGTTTTACGACCCAAGTGCCCTCCCTATGGTAGAACAGATTACTTTTAAAGATGTCATAGGCGATAATATCAAAACTGCAGGCCTCCTAGAGGGTTTAGAAGGTGGCAGTTTTCAGAACATTTGCCTATCCAATATTATCCTCAATGTAACATCAAAGTCCCCATGGAACTGCTCTTCTATTCATGGATATTCTTACTTTGTTTATCCAGAAATCTGTCAGCCTCTGAAGGAGAGTATCTCCCCTGAGCATTACTCGGATTGTTACCATCTATCAGATCAGAAATGGTTTTCCAGTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

467

Amino Acids

51.59

Weight (kDa)

5.81

Isoelectric Point (pI)

43.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 148 - 428 1.2e-42 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 407, 574
AccI GTMKAC 1 cut(s) 654
AciI CCGC 1 cut(s) 279
AclWI GGATC 4 cut(s) 367, 386, 722, 731
AcoI YGGCCR 2 cut(s) 283, 380
AcsI RAATTY 4 cut(s) 141, 170, 625, 894
AcuI CTGAAG 1 cut(s) 1346
AfaI GTAC 1 cut(s) 48
AfiI CCNNNNNNNGG 6 cut(s) 285, 407, 574, 1001, 1126, 1189
AgeI ACCGGT 1 cut(s) 65
AgsI TTSAA 1 cut(s) 758
AjnI CCWGG 1 cut(s) 994
AluBI AGCT 2 cut(s) 268, 303
AluI AGCT 2 cut(s) 268, 303
Alw21I GWGCWC 1 cut(s) 73
AlwI GGATC 4 cut(s) 367, 386, 722, 731
AlwNI CAGNNNCTG 1 cut(s) 1324
AoxI GGCC 6 cut(s) 210, 261, 283, 380, 563, 1180
ApeKI GCWGC 3 cut(s) 86, 516, 660
ApoI RAATTY 4 cut(s) 141, 170, 625, 894
AsiGI ACCGGT 1 cut(s) 65
Asp700I GAANNNNTTC 1 cut(s) 533
AspLEI GCGC 1 cut(s) 179
AspS9I GGNCC 3 cut(s) 261, 564, 639
AsuHPI GGTGA 8 cut(s) 55, 151, 197, 621, 638, 699, 791, 1063
AvaII GGWCC 1 cut(s) 639
BaeGI GKGCMC 2 cut(s) 462, 1120
BalI TGGCCA 1 cut(s) 382
BbsI GAAGAC 1 cut(s) 765
Bbv12I GWGCWC 1 cut(s) 73
BbvI GCAGC 3 cut(s) 73, 528, 672
BcgI CGANNNNNNTGC 4 cut(s) 879, 913, 1097, 1131
BciT130I CCWGG 1 cut(s) 996
BfaI CTAG 2 cut(s) 594, 1187
BfmI CTRYAG 2 cut(s) 658, 1176
BglII AGATCT 1 cut(s) 11
BisI GCNGC 3 cut(s) 87, 517, 661
BlsI GCNGC 3 cut(s) 88, 518, 662
Bme1390I CCNGG 1 cut(s) 996
Bme18I GGWCC 1 cut(s) 639
BmgT120I GGNCC 3 cut(s) 261, 564, 639
BmiI GGNNCC 3 cut(s) 262, 536, 566
BmrFI CCNGG 1 cut(s) 996
BmrI ACTGGG 2 cut(s) 644, 834
BmsI GCATC 1 cut(s) 334
BmuI ACTGGG 2 cut(s) 644, 834
BpiI GAAGAC 1 cut(s) 765
BplI GAGNNNNNCTC 6 cut(s) 183, 215, 1322, 1354, 1337, 1369
BpmI CTGGAG 2 cut(s) 939, 978
Bpu10I CCTNAGC 1 cut(s) 1344
Bsa29I ATCGAT 1 cut(s) 726
BsaBI GATNNNNATC 1 cut(s) 794
BsaJI CCNNGG 4 cut(s) 80, 207, 1041, 1262
BsaWI WCCGGW 1 cut(s) 65
BsaXI ACNNNNNCTCC 2 cut(s) 55, 85
Bsc4I CCNNNNNNNGG 6 cut(s) 285, 407, 574, 1001, 1126, 1189
Bse118I RCCGGY 2 cut(s) 65, 285
Bse1I ACTGG 7 cut(s) 383, 490, 650, 781, 840, 1074, 1392
Bse3DI GCAATG 1 cut(s) 109
Bse8I GATNNNNATC 1 cut(s) 794
BseBI CCWGG 1 cut(s) 996
BseCI ATCGAT 1 cut(s) 726
BseDI CCNNGG 4 cut(s) 80, 207, 1041, 1262
BseGI GGATG 6 cut(s) 40, 103, 343, 724, 817, 1051
BseJI GATNNNNATC 1 cut(s) 794
BseLI CCNNNNNNNGG 6 cut(s) 285, 407, 574, 1001, 1126, 1189
BseMI GCAATG 1 cut(s) 109
BseMII CTCAG 2 cut(s) 384, 1335
BseNI ACTGG 7 cut(s) 383, 490, 650, 781, 840, 1074, 1392
BseRI GAGGAG 3 cut(s) 19, 22, 687
BseSI GKGCMC 2 cut(s) 462, 1120
BseXI GCAGC 3 cut(s) 73, 528, 672
BsgI GTGCAG 1 cut(s) 909
BshFI GGCC 6 cut(s) 212, 263, 285, 382, 565, 1182
BshTI ACCGGT 1 cut(s) 65
BshVI ATCGAT 1 cut(s) 726
BsiHKAI GWGCWC 1 cut(s) 73
BsiSI CCGG 2 cut(s) 66, 286
BslFI GGGAC 3 cut(s) 260, 822, 1243
BslI CCNNNNNNNGG 6 cut(s) 285, 407, 574, 1001, 1126, 1189
BsmFI GGGAC 3 cut(s) 260, 822, 1243
BsmI GAATGC 1 cut(s) 226
BsnI GGCC 6 cut(s) 212, 263, 285, 382, 565, 1182
Bsp1286I GDGCHC 3 cut(s) 73, 462, 1120
Bsp143I GATC 8 cut(s) 11, 359, 391, 723, 727, 784, 789, 1375
Bsp19I CCATGG 2 cut(s) 80, 1262
BspACI CCGC 1 cut(s) 279
BspANI GGCC 6 cut(s) 212, 263, 285, 382, 565, 1182
BspCNI CTCAG 2 cut(s) 383, 1336
BspDI ATCGAT 1 cut(s) 726
BspLI GGNNCC 3 cut(s) 262, 536, 566
BspMAI CTGCAG 2 cut(s) 662, 1180
BspPI GGATC 4 cut(s) 367, 386, 722, 731
BspQI GCTCTTC 1 cut(s) 1279
BsrDI GCAATG 1 cut(s) 109
BsrFI RCCGGY 2 cut(s) 65, 285
BsrI ACTGG 7 cut(s) 383, 490, 650, 781, 840, 1074, 1392
BssAI RCCGGY 2 cut(s) 65, 285
BssECI CCNNGG 4 cut(s) 80, 207, 1041, 1262
BssMI GATC 8 cut(s) 11, 359, 391, 723, 727, 784, 789, 1375
BssNAI GTATAC 1 cut(s) 655
BssT1I CCWWGG 4 cut(s) 80, 207, 1041, 1262
Bst1107I GTATAC 1 cut(s) 655
Bst2UI CCWGG 1 cut(s) 996
Bst4CI ACNGT 2 cut(s) 155, 764
Bst6I CTCTTC 2 cut(s) 742, 1279
BstC8I GCNNGC 2 cut(s) 283, 1180
BstDEI CTNAG 2 cut(s) 370, 1344
BstDSI CCRYGG 2 cut(s) 80, 1262
BstENI CCTNNNNNAGG 1 cut(s) 1187
BstF5I GGATG 6 cut(s) 40, 103, 343, 724, 817, 1051
BstHHI GCGC 1 cut(s) 179
BstKTI GATC 8 cut(s) 14, 362, 394, 726, 730, 787, 792, 1378
BstMBI GATC 8 cut(s) 11, 359, 391, 723, 727, 784, 789, 1375
BstNI CCWGG 1 cut(s) 996
BstSCI CCNGG 1 cut(s) 994
BstSFI CTRYAG 2 cut(s) 658, 1176
BstSLI GKGCMC 2 cut(s) 462, 1120
BstV1I GCAGC 3 cut(s) 73, 528, 672
BstV2I GAAGAC 1 cut(s) 765
BstX2I RGATCY 1 cut(s) 11
BstXI CCANNNNNNTGG 1 cut(s) 959
BstYI RGATCY 1 cut(s) 11
BstZ17I GTATAC 1 cut(s) 655
Bsu15I ATCGAT 1 cut(s) 726
BsuRI GGCC 6 cut(s) 212, 263, 285, 382, 565, 1182
BsuTUI ATCGAT 1 cut(s) 726
BtgI CCRYGG 2 cut(s) 80, 1262
BtsCI GGATG 6 cut(s) 40, 103, 343, 724, 817, 1051
BtsIMutI CAGTG 4 cut(s) 160, 165, 774, 1399
Cac8I GCNNGC 2 cut(s) 283, 1180
CaiI CAGNNNCTG 1 cut(s) 1324
CfoI GCGC 1 cut(s) 179
Cfr10I RCCGGY 2 cut(s) 65, 285
Cfr13I GGNCC 3 cut(s) 261, 564, 639
ClaI ATCGAT 1 cut(s) 726
Csp6I GTAC 1 cut(s) 47
CspAI ACCGGT 1 cut(s) 65
CviAII CATG 5 cut(s) 81, 665, 938, 1263, 1283
CviQI GTAC 1 cut(s) 47
DdeI CTNAG 2 cut(s) 370, 1344
DpnI GATC 8 cut(s) 13, 361, 393, 725, 729, 786, 791, 1377
DpnII GATC 8 cut(s) 11, 359, 391, 723, 727, 784, 789, 1375
DraI TTTAAA 2 cut(s) 624, 1147
EaeI YGGCCR 2 cut(s) 283, 380
Eam1104I CTCTTC 2 cut(s) 742, 1279
EarI CTCTTC 2 cut(s) 742, 1279
Eco130I CCWWGG 4 cut(s) 80, 207, 1041, 1262
Eco147I AGGCCT 2 cut(s) 212, 1182
Eco47I GGWCC 1 cut(s) 639
Eco57I CTGAAG 1 cut(s) 1346
EcoNI CCTNNNNNAGG 1 cut(s) 1187
EcoO109I RGGNCCY 1 cut(s) 564
EcoRII CCWGG 1 cut(s) 994
EcoT14I CCWWGG 4 cut(s) 80, 207, 1041, 1262
ErhI CCWWGG 4 cut(s) 80, 207, 1041, 1262
FaeI CATG 5 cut(s) 84, 668, 941, 1266, 1286
FaqI GGGAC 3 cut(s) 260, 822, 1243
FatI CATG 5 cut(s) 80, 664, 937, 1262, 1282
FauI CCCGC 1 cut(s) 286
FauNDI CATATG 2 cut(s) 406, 829
FblI GTMKAC 1 cut(s) 654
Fnu4HI GCNGC 3 cut(s) 87, 517, 661
FokI GGATG 6 cut(s) 47, 90, 350, 731, 824, 1058
Fsp4HI GCNGC 3 cut(s) 87, 517, 661
FspBI CTAG 2 cut(s) 594, 1187
GlaI GCGC 1 cut(s) 178
GluI GCNGC 3 cut(s) 87, 517, 661
GsuI CTGGAG 2 cut(s) 939, 978
HaeIII GGCC 6 cut(s) 212, 263, 285, 382, 565, 1182
HapII CCGG 2 cut(s) 66, 286
HhaI GCGC 1 cut(s) 179
Hin1II CATG 5 cut(s) 84, 668, 941, 1266, 1286
Hin6I GCGC 1 cut(s) 177
HinP1I GCGC 1 cut(s) 177
HincII GTYRAC 1 cut(s) 294
HindII GTYRAC 1 cut(s) 294
HindIII AAGCTT 2 cut(s) 266, 301
HinfI GANTC 3 cut(s) 192, 679, 734
HpaII CCGG 2 cut(s) 66, 286
HphI GGTGA 8 cut(s) 55, 151, 197, 621, 638, 699, 791, 1063
Hpy166II GTNNAC 4 cut(s) 63, 294, 655, 1071
Hpy188III TCNNGA 4 cut(s) 676, 731, 918, 1307
Hpy8I GTNNAC 4 cut(s) 63, 294, 655, 1071
HpyAV CCTTC 4 cut(s) 411, 993, 1193, 1321
HpyCH4III ACNGT 2 cut(s) 155, 764
HpyCH4V TGCA 8 cut(s) 56, 114, 347, 660, 753, 890, 941, 1178
HpyF3I CTNAG 2 cut(s) 370, 1344
Hsp92II CATG 5 cut(s) 84, 668, 941, 1266, 1286
HspAI GCGC 1 cut(s) 177
Kzo9I GATC 8 cut(s) 11, 359, 391, 723, 727, 784, 789, 1375
LguI GCTCTTC 1 cut(s) 1279
LmnI GCTCC 2 cut(s) 76, 700
Lsp1109I GCAGC 3 cut(s) 73, 528, 672
LweI GCATC 1 cut(s) 334
MaeI CTAG 2 cut(s) 594, 1187
MaeIII GTNAC 6 cut(s) 157, 193, 539, 1076, 1246, 1361
MalI GATC 8 cut(s) 13, 361, 393, 725, 729, 786, 791, 1377
MboI GATC 8 cut(s) 11, 359, 391, 723, 727, 784, 789, 1375
MboII GAAGA 6 cut(s) 6, 220, 353, 729, 770, 1266
MflI RGATCY 1 cut(s) 11
MhlI GDGCHC 3 cut(s) 73, 462, 1120
MlsI TGGCCA 1 cut(s) 382
MluNI TGGCCA 1 cut(s) 382
MlyI GAGTC 2 cut(s) 201, 728
MmeI TCCRAC 1 cut(s) 315
Mox20I TGGCCA 1 cut(s) 382
MroXI GAANNNNTTC 1 cut(s) 533
MscI TGGCCA 1 cut(s) 382
MseI TTAA 3 cut(s) 122, 623, 1146
MslI CAYNNNNRTG 1 cut(s) 1095
Msp20I TGGCCA 1 cut(s) 382
MspA1I CMGCKG 1 cut(s) 281
MspI CCGG 2 cut(s) 66, 286
MspR9I CCNGG 1 cut(s) 996
Mva1269I GAATGC 1 cut(s) 226
MvaI CCWGG 1 cut(s) 996
NcoI CCATGG 2 cut(s) 80, 1262
NdeI CATATG 2 cut(s) 406, 829
NdeII GATC 8 cut(s) 11, 359, 391, 723, 727, 784, 789, 1375
NlaIII CATG 5 cut(s) 84, 668, 941, 1266, 1286
NlaIV GGNNCC 3 cut(s) 262, 536, 566
NmuCI GTSAC 3 cut(s) 157, 193, 1076
PceI AGGCCT 2 cut(s) 212, 1182
PciSI GCTCTTC 1 cut(s) 1279
PctI GAATGC 1 cut(s) 226
PdmI GAANNNNTTC 1 cut(s) 533
PfeI GAWTC 1 cut(s) 679
PflMI CCANNNNNTGG 2 cut(s) 407, 574
PfoI TCCNGGA 1 cut(s) 994
PinAI ACCGGT 1 cut(s) 65
PkrI GCNGC 3 cut(s) 88, 518, 662
PleI GAGTC 2 cut(s) 200, 728
PpsI GAGTC 2 cut(s) 200, 728
Psp6I CCWGG 1 cut(s) 994
PspGI CCWGG 1 cut(s) 994
PspN4I GGNNCC 3 cut(s) 262, 536, 566
PspPI GGNCC 3 cut(s) 261, 564, 639
PstI CTGCAG 2 cut(s) 662, 1180
PstNI CAGNNNCTG 1 cut(s) 1324
PsuI RGATCY 1 cut(s) 11
RsaI GTAC 1 cut(s) 48
RsaNI GTAC 1 cut(s) 47
RseI CAYNNNNRTG 1 cut(s) 1095
SapI GCTCTTC 1 cut(s) 1279
SaqAI TTAA 3 cut(s) 122, 623, 1146
SatI GCNGC 3 cut(s) 87, 517, 661
Sau3AI GATC 8 cut(s) 11, 359, 391, 723, 727, 784, 789, 1375
Sau96I GGNCC 3 cut(s) 261, 564, 639
SchI GAGTC 2 cut(s) 201, 728
ScrFI CCNGG 1 cut(s) 996
SduI GDGCHC 3 cut(s) 73, 462, 1120
SfaNI GCATC 1 cut(s) 334
SfcI CTRYAG 2 cut(s) 658, 1176
SgrAI CRCCGGYG 1 cut(s) 65
SinI GGWCC 1 cut(s) 639
SmiMI CAYNNNNRTG 1 cut(s) 1095
SseBI AGGCCT 2 cut(s) 212, 1182
SsiI CCGC 1 cut(s) 279
SspI AATATT 1 cut(s) 1234
SspMI CTAG 2 cut(s) 594, 1187
StuI AGGCCT 2 cut(s) 212, 1182
StyD4I CCNGG 1 cut(s) 994
StyI CCWWGG 4 cut(s) 80, 207, 1041, 1262
TaaI ACNGT 2 cut(s) 155, 764
TaqI TCGA 4 cut(s) 145, 412, 726, 899
TaqII GACCGA 1 cut(s) 485
TfiI GAWTC 1 cut(s) 679
Tru1I TTAA 3 cut(s) 122, 623, 1146
Tru9I TTAA 3 cut(s) 122, 623, 1146
TscAI CASTG 4 cut(s) 160, 172, 781, 1399
TseFI GTSAC 3 cut(s) 157, 193, 1076
TseI GCWGC 3 cut(s) 86, 516, 660
Tsp45I GTSAC 3 cut(s) 157, 193, 1076
TspDTI ATGAA 5 cut(s) 354, 599, 816, 926, 1271
TspGWI ACGGA 4 cut(s) 63, 527, 661, 731
TspRI CASTG 4 cut(s) 160, 172, 781, 1399
Van91I CCANNNNNTGG 2 cut(s) 407, 574
VpaK11BI GGWCC 1 cut(s) 639
XagI CCTNNNNNAGG 1 cut(s) 1187
XapI RAATTY 4 cut(s) 141, 170, 625, 894
XmiI GTMKAC 1 cut(s) 654
XmnI GAANNNNTTC 1 cut(s) 533
XspI CTAG 2 cut(s) 594, 1187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.