MD09G1290500.v1.1

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0007246
Physical Location & Seq
Forward (+)
37245074 .. 37248834
3761 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1290500.v1.1.491

Sequence Viewer

Length: 1440 bp
ATGATGAGGACGACGACATCTTCTTCTTCTTCCACTCTCACTGTCACTGTGGTGGACGTGCTTCTGCTACTTGCATTGCTAGGCGCTGCTGCTCCGTCGTCTGTCAAAGGCAGCCCAAAGTGCAGCAATCAGGCCAACTCTGGGGAAATTCGACCTCACAGTGTCGCCATCACTGAATTTGGCGCTGTAGGAGATGGGGTCACTCTCAACACAAAAGCCTTTCAGAATGCCGTCTTCTATCTCAACTCTTTCACACGCAAGGGTGGGGCCAAGCTTTTTGTCCCTGCTGGCCGCTGGTTGACAGGAAGCTTCCGTCTCATCAGTCATCTAACCCTCTGGTTGGATAAGGACGCTGTCATTCTTGGATCAACGAAATCCGATGATTGGCCAATTGTTGAACCATTACCATCGTACGGTCGAGGAAGGGAGTTGGCAGGAGGAAGACATCGAAGTCTCATCTATGGATGCGATTTGACAGACATTATCATAACAGGTGATAATGGTACCATTGATGGCCAAGGTAGCATCTGGTGGGATTGGTTCCATAGCAAAACCCTGAATTATACGAGGCCCCATTTGTTGGAATTGATAAACTCAACTGGGGTTGTCATCTCAAACTTGACCTTCCTAAATTCACCATTTTGGACCATTCACCCGGTATATTGCAGCCATGTAACTATCCAGAATCTGACAATCCTTGCTCCTCTCAAGTCTCCAAACACAGATGGGATTGATCCAGATTCTTCAGATAATGTGTGCATTGAAGACTGTTATATTAGCACTGGTGATGATCTGATTTCCATCAAAAGTGGGTGGGATGAGTATGGAATTTCATATGGTCGACCCAGTAGAAACATAATTATTCACAGAATTACCGGTAAAACTGAAACAAGCGCTGGAATTGCAATTGGAAGTGAGATGTCAGGAGGTGTGTCAGAAGTTCATGTAGAAGACCTTCACTTTTTCGGTTCTAAAACAGGTATCAGGATAAAGACCTCCCCAGGAAGGGGTGGTTATGTGAGAAATATATATGTATCAAACATGAACTTGGATGGTGTAGGCATAGCTTTAAGGTTCACCAGTCAGTATGGGGAGCACCCAGATGAGTTTTACAACCCAAATGCCCTCCCTAAAGTAGAGAGAATTACTTTTAAAAATATCATAGGAGAGAATATCAAAACTGCTGGTCTCCTAGAGGGTTTAGAAGGTGAAAAATTTCTGAACATTTGCCTATCTAATATTACCCTTAACGTGAGATCAAAATCTCCATGGAAATGCTCTTCTGTTCAAGGATATTCCGACCTAGTTTCTCCAGAAACTTGTGCGCCTCTCAAGGAGAATATCTCCCCACGGCATTATTCAGATTGTTACTCTCTATCTGATGACACATGGATTTCTTCCAGTAATCAAAACAGAGGTGCTCGGTGGCTATCTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

480

Amino Acids

52.37

Weight (kDa)

6.32

Isoelectric Point (pI)

39.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 151 - 430 1e-43 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 450
Acc65I GGTACC 1 cut(s) 503
AccB1I GGYRCC 1 cut(s) 503
AccB7I CCANNNNNTGG 1 cut(s) 580
AccI GTMKAC 1 cut(s) 841
AciI CCGC 1 cut(s) 292
AclWI GGATC 2 cut(s) 373, 728
AcoI YGGCCR 3 cut(s) 289, 386, 514
AcsI RAATTY 5 cut(s) 147, 176, 631, 828, 1214
AcuI CTGAAG 1 cut(s) 729
AfaI GTAC 2 cut(s) 413, 505
AfeI AGCGCT 1 cut(s) 895
AfiI CCNNNNNNNGG 8 cut(s) 141, 340, 384, 413, 580, 1005, 1006, 1007
AgeI ACCGGT 1 cut(s) 875
AgsI TTSAA 3 cut(s) 398, 764, 1289
AjiI CACGTC 1 cut(s) 58
AjnI CCWGG 1 cut(s) 1000
AleI CACNNNNGTG 1 cut(s) 50
AluBI AGCT 3 cut(s) 274, 309, 1067
AluI AGCT 3 cut(s) 274, 309, 1067
Alw21I GWGCWC 2 cut(s) 1098, 1423
Alw26I GTCTC 4 cut(s) 320, 458, 717, 1193
AlwI GGATC 2 cut(s) 373, 728
AlwNI CAGNNNCTG 1 cut(s) 688
Aor51HI AGCGCT 1 cut(s) 895
AoxI GGCC 6 cut(s) 132, 267, 289, 386, 514, 569
ApeKI GCWGC 5 cut(s) 86, 89, 111, 123, 666
ApoI RAATTY 5 cut(s) 147, 176, 631, 828, 1214
AsiGI ACCGGT 1 cut(s) 875
Asp700I GAANNNNTTC 2 cut(s) 954, 1215
Asp718I GGTACC 1 cut(s) 503
AspLEI GCGC 4 cut(s) 86, 185, 896, 1327
AspS9I GGNCC 3 cut(s) 267, 570, 645
AsuC2I CCSGG 1 cut(s) 656
AsuHPI GGTGA 6 cut(s) 506, 627, 644, 797, 1069, 1220
AvaII GGWCC 1 cut(s) 645
BalI TGGCCA 2 cut(s) 388, 516
BanI GGYRCC 1 cut(s) 503
BarI GAAGNNNNNNTAC 2 cut(s) 939, 971
BbsI GAAGAC 4 cut(s) 226, 448, 771, 957
Bbv12I GWGCWC 2 cut(s) 1098, 1423
BbvI GCAGC 5 cut(s) 73, 76, 123, 135, 678
BccI CCATC 7 cut(s) 176, 188, 415, 506, 719, 809, 1046
BceAI ACGGC 2 cut(s) 215, 1367
BciT130I CCWGG 1 cut(s) 1002
BcnI CCSGG 1 cut(s) 656
BcoDI GTCTC 4 cut(s) 320, 458, 717, 1193
BfaI CTAG 3 cut(s) 80, 1193, 1304
BfmI CTRYAG 1 cut(s) 186
BfoI RGCGCY 3 cut(s) 87, 186, 897
BisI GCNGC 6 cut(s) 87, 90, 112, 124, 292, 667
BlsI GCNGC 6 cut(s) 88, 91, 113, 125, 293, 668
Bme1390I CCNGG 2 cut(s) 656, 1002
Bme18I GGWCC 1 cut(s) 645
BmgBI CACGTC 1 cut(s) 58
BmgT120I GGNCC 3 cut(s) 267, 570, 645
BmiI GGNNCC 4 cut(s) 268, 505, 542, 572
BmrFI CCNGG 2 cut(s) 656, 1002
BmrI ACTGGG 2 cut(s) 609, 840
BmsI GCATC 2 cut(s) 455, 534
BmuI ACTGGG 2 cut(s) 609, 840
BpiI GAAGAC 4 cut(s) 226, 448, 771, 957
BplI GAGNNNNNCTC 2 cut(s) 1328, 1360
BpmI CTGGAG 1 cut(s) 1296
BpuEI CTTGAG 2 cut(s) 692, 1316
BpuMI CCSGG 1 cut(s) 656
BsaBI GATNNNNATC 2 cut(s) 800, 1261
BsaI GGTCTC 1 cut(s) 1193
BsaJI CCNNGG 4 cut(s) 517, 1000, 1268, 1349
BsaWI WCCGGW 1 cut(s) 875
BsaXI ACNNNNNCTCC 2 cut(s) 183, 213
Bsc4I CCNNNNNNNGG 8 cut(s) 141, 340, 384, 413, 580, 1005, 1006, 1007
Bse118I RCCGGY 1 cut(s) 875
Bse1I ACTGG 5 cut(s) 604, 787, 846, 1080, 1401
Bse3DI GCAATG 1 cut(s) 74
Bse8I GATNNNNATC 2 cut(s) 800, 1261
BseBI CCWGG 1 cut(s) 1002
BseDI CCNNGG 4 cut(s) 517, 1000, 1268, 1349
BseGI GGATG 3 cut(s) 470, 823, 1057
BseJI GATNNNNATC 2 cut(s) 800, 1261
BseLI CCNNNNNNNGG 8 cut(s) 141, 340, 384, 413, 580, 1005, 1006, 1007
BseMI GCAATG 1 cut(s) 74
BseNI ACTGG 5 cut(s) 604, 787, 846, 1080, 1401
BseRI GAGGAG 1 cut(s) 693
BseXI GCAGC 5 cut(s) 73, 76, 123, 135, 678
BsgI GTGCAG 1 cut(s) 142
Bsh1285I CGRYCG 1 cut(s) 418
BshFI GGCC 6 cut(s) 134, 269, 291, 388, 516, 571
BshNI GGYRCC 1 cut(s) 503
BshTI ACCGGT 1 cut(s) 875
BsiEI CGRYCG 1 cut(s) 418
BsiHKAI GWGCWC 2 cut(s) 1098, 1423
BsiSI CCGG 2 cut(s) 656, 876
BsiWI CGTACG 1 cut(s) 411
BslFI GGGAC 1 cut(s) 266
BslI CCNNNNNNNGG 8 cut(s) 141, 340, 384, 413, 580, 1005, 1006, 1007
BsmAI GTCTC 4 cut(s) 320, 458, 717, 1193
BsmBI CGTCTC 1 cut(s) 320
BsmFI GGGAC 1 cut(s) 266
BsmI GAATGC 1 cut(s) 232
BsnI GGCC 6 cut(s) 134, 269, 291, 388, 516, 571
Bso31I GGTCTC 1 cut(s) 1193
Bsp1286I GDGCHC 2 cut(s) 1098, 1423
Bsp143I GATC 4 cut(s) 365, 733, 790, 1256
Bsp19I CCATGG 1 cut(s) 1268
BspACI CCGC 1 cut(s) 292
BspANI GGCC 6 cut(s) 134, 269, 291, 388, 516, 571
BspLI GGNNCC 4 cut(s) 268, 505, 542, 572
BspPI GGATC 2 cut(s) 373, 728
BspQI GCTCTTC 1 cut(s) 1285
BspT107I GGYRCC 1 cut(s) 503
BspTNI GGTCTC 1 cut(s) 1193
BsrDI GCAATG 1 cut(s) 74
BsrFI RCCGGY 1 cut(s) 875
BsrI ACTGG 5 cut(s) 604, 787, 846, 1080, 1401
BssAI RCCGGY 1 cut(s) 875
BssECI CCNNGG 4 cut(s) 517, 1000, 1268, 1349
BssMI GATC 4 cut(s) 365, 733, 790, 1256
BssT1I CCWWGG 2 cut(s) 517, 1268
Bst2UI CCWGG 1 cut(s) 1002
Bst4CI ACNGT 5 cut(s) 43, 49, 161, 416, 770
Bst6I CTCTTC 1 cut(s) 1285
BstC8I GCNNGC 1 cut(s) 289
BstDSI CCRYGG 2 cut(s) 1268, 1349
BstF5I GGATG 3 cut(s) 470, 823, 1057
BstH2I RGCGCY 3 cut(s) 87, 186, 897
BstHHI GCGC 4 cut(s) 86, 185, 896, 1327
BstKTI GATC 4 cut(s) 368, 736, 793, 1259
BstMAI GTCTC 4 cut(s) 320, 458, 717, 1193
BstMBI GATC 4 cut(s) 365, 733, 790, 1256
BstMCI CGRYCG 1 cut(s) 418
BstMWI GCNNNNNNNGC 3 cut(s) 120, 522, 902
BstNI CCWGG 1 cut(s) 1002
BstSCI CCNGG 2 cut(s) 654, 1000
BstSFI CTRYAG 1 cut(s) 186
BstV1I GCAGC 5 cut(s) 73, 76, 123, 135, 678
BstV2I GAAGAC 4 cut(s) 226, 448, 771, 957
BsuRI GGCC 6 cut(s) 134, 269, 291, 388, 516, 571
BtgI CCRYGG 2 cut(s) 1268, 1349
BtrI CACGTC 1 cut(s) 58
BtsCI GGATG 3 cut(s) 470, 823, 1057
BtsIMutI CAGTG 5 cut(s) 39, 45, 166, 171, 780
Cac8I GCNNGC 1 cut(s) 289
CaiI CAGNNNCTG 1 cut(s) 688
CfoI GCGC 4 cut(s) 86, 185, 896, 1327
Cfr10I RCCGGY 1 cut(s) 875
Cfr13I GGNCC 3 cut(s) 267, 570, 645
CseI GACGC 1 cut(s) 359
Csp6I GTAC 2 cut(s) 412, 504
CspAI ACCGGT 1 cut(s) 875
CviAII CATG 5 cut(s) 671, 944, 1042, 1269, 1389
CviQI GTAC 2 cut(s) 412, 504
DpnI GATC 4 cut(s) 367, 735, 792, 1258
DpnII GATC 4 cut(s) 365, 733, 790, 1256
DraI TTTAAA 1 cut(s) 1153
DrdI GACNNNNNNGTC 1 cut(s) 450
DseDI GACNNNNNNGTC 1 cut(s) 450
EaeI YGGCCR 3 cut(s) 289, 386, 514
Eam1104I CTCTTC 1 cut(s) 1285
EarI CTCTTC 1 cut(s) 1285
Eco130I CCWWGG 2 cut(s) 517, 1268
Eco31I GGTCTC 1 cut(s) 1193
Eco47I GGWCC 1 cut(s) 645
Eco47III AGCGCT 1 cut(s) 895
Eco57I CTGAAG 1 cut(s) 729
EcoO109I RGGNCCY 1 cut(s) 570
EcoRII CCWGG 1 cut(s) 1000
EcoT14I CCWWGG 2 cut(s) 517, 1268
ErhI CCWWGG 2 cut(s) 517, 1268
Esp3I CGTCTC 1 cut(s) 320
FaeI CATG 5 cut(s) 674, 947, 1045, 1272, 1392
FaqI GGGAC 1 cut(s) 266
FatI CATG 5 cut(s) 670, 943, 1041, 1268, 1388
FauNDI CATATG 1 cut(s) 835
FblI GTMKAC 1 cut(s) 841
Fnu4HI GCNGC 6 cut(s) 87, 90, 112, 124, 292, 667
FokI GGATG 3 cut(s) 477, 830, 1064
Fsp4HI GCNGC 6 cut(s) 87, 90, 112, 124, 292, 667
FspBI CTAG 3 cut(s) 80, 1193, 1304
GlaI GCGC 4 cut(s) 85, 184, 895, 1326
GluI GCNGC 6 cut(s) 87, 90, 112, 124, 292, 667
GsuI CTGGAG 1 cut(s) 1296
HaeII RGCGCY 3 cut(s) 87, 186, 897
HaeIII GGCC 6 cut(s) 134, 269, 291, 388, 516, 571
HapII CCGG 2 cut(s) 656, 876
HgaI GACGC 1 cut(s) 359
HhaI GCGC 4 cut(s) 86, 185, 896, 1327
Hin1II CATG 5 cut(s) 674, 947, 1045, 1272, 1392
Hin6I GCGC 4 cut(s) 84, 183, 894, 1325
HinP1I GCGC 4 cut(s) 84, 183, 894, 1325
HincII GTYRAC 2 cut(s) 300, 842
HindII GTYRAC 2 cut(s) 300, 842
HindIII AAGCTT 2 cut(s) 272, 307
HinfI GANTC 2 cut(s) 685, 740
HpaII CCGG 2 cut(s) 656, 876
HphI GGTGA 6 cut(s) 506, 627, 644, 797, 1069, 1220
Hpy166II GTNNAC 4 cut(s) 55, 300, 842, 1077
Hpy188III TCNNGA 5 cut(s) 682, 737, 924, 985, 1313
Hpy8I GTNNAC 4 cut(s) 55, 300, 842, 1077
Hpy99I CGWCG 2 cut(s) 16, 100
HpyAV CCTTC 5 cut(s) 417, 634, 965, 999, 1199
HpyCH4III ACNGT 5 cut(s) 43, 49, 161, 416, 770
HpyCH4IV ACGT 2 cut(s) 57, 1251
HpyCH4V TGCA 5 cut(s) 74, 123, 666, 759, 905
HpyF10VI GCNNNNNNNGC 3 cut(s) 120, 522, 902
HpySE526I ACGT 2 cut(s) 57, 1251
Hsp92II CATG 5 cut(s) 674, 947, 1045, 1272, 1392
HspAI GCGC 4 cut(s) 84, 183, 894, 1325
KpnI GGTACC 1 cut(s) 507
Kzo9I GATC 4 cut(s) 365, 733, 790, 1256
LguI GCTCTTC 1 cut(s) 1285
LmnI GCTCC 3 cut(s) 97, 706, 1093
Lsp1109I GCAGC 5 cut(s) 73, 76, 123, 135, 678
LweI GCATC 2 cut(s) 455, 534
MaeI CTAG 3 cut(s) 80, 1193, 1304
MaeII ACGT 2 cut(s) 57, 1251
MaeIII GTNAC 4 cut(s) 43, 199, 673, 1367
MalI GATC 4 cut(s) 367, 735, 792, 1258
MboI GATC 4 cut(s) 365, 733, 790, 1256
MfeI CAATTG 2 cut(s) 390, 906
MhlI GDGCHC 2 cut(s) 1098, 1423
MlsI TGGCCA 2 cut(s) 388, 516
MluNI TGGCCA 2 cut(s) 388, 516
MmeI TCCRAC 3 cut(s) 321, 561, 1323
Mox20I TGGCCA 2 cut(s) 388, 516
MroXI GAANNNNTTC 2 cut(s) 954, 1215
MscI TGGCCA 2 cut(s) 388, 516
MseI TTAA 3 cut(s) 1070, 1152, 1248
MslI CAYNNNNRTG 3 cut(s) 50, 1101, 1273
Msp20I TGGCCA 2 cut(s) 388, 516
MspA1I CMGCKG 1 cut(s) 294
MspI CCGG 2 cut(s) 656, 876
MspR9I CCNGG 2 cut(s) 656, 1002
MunI CAATTG 2 cut(s) 390, 906
Mva1269I GAATGC 1 cut(s) 232
MvaI CCWGG 1 cut(s) 1002
MwoI GCNNNNNNNGC 3 cut(s) 120, 522, 902
NciI CCSGG 1 cut(s) 656
NcoI CCATGG 1 cut(s) 1268
NdeI CATATG 1 cut(s) 835
NdeII GATC 4 cut(s) 365, 733, 790, 1256
NlaIII CATG 5 cut(s) 674, 947, 1045, 1272, 1392
NlaIV GGNNCC 4 cut(s) 268, 505, 542, 572
NmuCI GTSAC 2 cut(s) 43, 199
OliI CACNNNNGTG 1 cut(s) 50
PciSI GCTCTTC 1 cut(s) 1285
PctI GAATGC 1 cut(s) 232
PdmI GAANNNNTTC 2 cut(s) 954, 1215
PfeI GAWTC 2 cut(s) 685, 740
Pfl23II CGTACG 1 cut(s) 411
PflFI GACNNNGTC 1 cut(s) 353
PflMI CCANNNNNTGG 1 cut(s) 580
PinAI ACCGGT 1 cut(s) 875
PkrI GCNGC 6 cut(s) 88, 91, 113, 125, 293, 668
Psp6I CCWGG 1 cut(s) 1000
PspGI CCWGG 1 cut(s) 1000
PspLI CGTACG 1 cut(s) 411
PspN4I GGNNCC 4 cut(s) 268, 505, 542, 572
PspPI GGNCC 3 cut(s) 267, 570, 645
PstNI CAGNNNCTG 1 cut(s) 688
PsyI GACNNNGTC 1 cut(s) 353
RsaI GTAC 2 cut(s) 413, 505
RsaNI GTAC 2 cut(s) 412, 504
RseI CAYNNNNRTG 3 cut(s) 50, 1101, 1273
SalI GTCGAC 1 cut(s) 840
SapI GCTCTTC 1 cut(s) 1285
SaqAI TTAA 3 cut(s) 1070, 1152, 1248
SatI GCNGC 6 cut(s) 87, 90, 112, 124, 292, 667
Sau3AI GATC 4 cut(s) 365, 733, 790, 1256
Sau96I GGNCC 3 cut(s) 267, 570, 645
ScrFI CCNGG 2 cut(s) 656, 1002
SduI GDGCHC 2 cut(s) 1098, 1423
SfaNI GCATC 2 cut(s) 455, 534
SfcI CTRYAG 1 cut(s) 186
SinI GGWCC 1 cut(s) 645
SmiMI CAYNNNNRTG 3 cut(s) 50, 1101, 1273
SmlI CTYRAG 2 cut(s) 707, 1331
SmoI CTYRAG 2 cut(s) 707, 1331
SsiI CCGC 1 cut(s) 292
SspI AATATT 1 cut(s) 1240
SspMI CTAG 3 cut(s) 80, 1193, 1304
StyD4I CCNGG 2 cut(s) 654, 1000
StyI CCWWGG 2 cut(s) 517, 1268
TaaI ACNGT 5 cut(s) 43, 49, 161, 416, 770
TaiI ACGT 2 cut(s) 60, 1254
TaqI TCGA 4 cut(s) 151, 418, 448, 841
TauI GCSGC 1 cut(s) 294
TfiI GAWTC 2 cut(s) 685, 740
Tru1I TTAA 3 cut(s) 1070, 1152, 1248
Tru9I TTAA 3 cut(s) 1070, 1152, 1248
TscAI CASTG 5 cut(s) 46, 52, 166, 178, 787
TseFI GTSAC 2 cut(s) 43, 199
TseI GCWGC 5 cut(s) 86, 89, 111, 123, 666
Tsp45I GTSAC 2 cut(s) 43, 199
TspDTI ATGAA 3 cut(s) 822, 932, 1058
TspGWI ACGGA 2 cut(s) 84, 302
TspRI CASTG 5 cut(s) 46, 52, 166, 178, 787
Tth111I GACNNNGTC 1 cut(s) 353
Van91I CCANNNNNTGG 1 cut(s) 580
VpaK11BI GGWCC 1 cut(s) 645
XapI RAATTY 5 cut(s) 147, 176, 631, 828, 1214
XmiI GTMKAC 1 cut(s) 841
XmnI GAANNNNTTC 2 cut(s) 954, 1215
XspI CTAG 3 cut(s) 80, 1193, 1304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.