Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0008324

Physical Location & Seq

Genomic Coordinates Reverse (-)

18679709 .. 18684067

Gene Structure 4359 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g26860.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2187 bp

ATGGGTACTGAGAGGAAGAGGAAGGTGAGCTTGTTCGACGTGGTGGACGAGAGCACAGTAGCGGCTGCGAAGATGAACAAATTGAACGGCGGCGGCGGCGGCGCCATGAATCAAAATCCGATGAGCAGTGTGATCAATAGGTGGACCGGAAAGCCATACTCGCAAAGGTACTATGAGATCCTTGAGAAGAGGAAGACTTTGCCTGTTTGGCACCAGAAGGAGGAGTTCTTTCAGGCCCTCAAGAAAAGCCAGTCGCTGATTTTGGTTGGTGAGACTGGTAGTGGTAAAACCACTCAGATCCCTCAATTTGTTTTGGAAGCTGTTGATCCGTCTTCAGATAAAAGCAGGAAGATGGTTGCTTGTACCCAGCCTCGTAGGGTGGCTGCAATGTCAGTCTCTCGCCGTGTTGCTGAAGAGATGGATGTTACCATTGGCGAAGAGGTTGGTTATAGCATTCGTTTTGAAGACTGCAGCAGTGCCAGAACAGTATTGAAGTATCTGACAGATGGTATGCTTTTAAGAGAAGCGATGACAGATCCCCTGTTGGAACGATACAGTGTAATTATTCTTGATGAGGCTCATGAGAGGACTTTAGCAACAGATGTTCTGTTTGGTCTTTTGAAGGAAGTATTGAAAAATAGACCTGATTTGAAACTGGTTGTTATGAGTGCTACACTTGAGGCTGAGAAGTTTCAGGGTTACTTCAATGGTGCACCACTAATGAAGGTTCCCGGCAGGCTTCATCCTGTTGAAATTTTTTACACTGAGGAACCTGAGAGGGACTACCTGGAGGCAGCAATTCGAACTGTTGTACAGATTCATATGTATGAGACTCCAGGAGATATACTTGTTTTTCTAACCGGAGAAGAGGAGATAGAAGATGCATGCCGCAAAATCAACAAAGAAATTTCAAACCTGGGTGATCAGGTTGGTCCTGTGAAAGTGGTGCCCTTGTATTCAACTCTCCCTCCAGCTATGCAGCAGAAAATATTTGACGCTGCTCCTCCTCCCACAACTGAAGGAGGTCCTGCAGGAAGGAAAATTGTGGTGTCAACTAACATTGCAGAAACTTCTCTTACAATAGATGGGATCGTGTATGTTATTGATCCAGGTTTTTCTAAACAGAAAGTTTATAACCCACGAGTGCGTGTTGAATCCTTGTTGGTATCTCCAATTTCTAAGGCTAGTGCGCATCAGAGGTCAGGGCGTGCTGGAAGAACTCAGCCAGGCAAATGTTTCCGACTTTATACTGAGAAGAGTTTTCATAATGATCTTGTACCGCAGACATATCCAGAAATACTGAGATCAAATCTTGCAAATACGGTGCTTACTTTGAAGAAACTAGGAATAGATGATTTGGTTCATTTTGATTTTATGGACCCCCCTGCTCCAGAGACATTAATGAGAGCACTTGAGGTTTTGAATTATTTGGGTGCATTGGATGACGATGGTAACTTGACCAAGCTGGGTGAGATAATGAGCGAGTTCCCTCTGGATCCTCAGATGTCAAAGATGCTTGTTGTTAGCCCTGAGTTCAACTGCTCAAATGAGATTTTGTCGATTTCTGCCATGCTTTCAGTACCCAATTGCTTTGTCCGGCCTAGGGAGGCTCAAAAAGCTGCTGACGAAGCAAAAGCTAGGTTTGGACACATCGATGGAGATCATCTCACACTGCTGAACGTGTATCATGCATACAAGCAAAACAATGAGGATCCATCGTGGTGCTATGAGAACTTCGTCAATCAGAGGGCATTGAAGTCTGCGGACAATGTTAGACAGCAGTTAGTCCGCATTATGGCTCGGTTTAATCTCAAGCAATGCAGTACTGATTTTAAGAGTCGGGACTACTACATCAACATCAGAAAGGCTATGCTAGCTGGGTACTTCATGCAGGTGGCTCATCTGGAACGCACTGGTCACTACCTTACTGTGAAGGATAACCAAGTTGTACATTTGCATCCATCAAATTGCCTGGATCACAAGCCAGAGTGGGTGATATACAATGAGTATGTCTTAACAAGCAGGAACTTTATCCGCACAGTTACAGATATCCGGGGTGAATGGCTAGTTGATATAGCACCGCACTACTATGATTTGGCCAACTTTCCCCAGTGTGAGGCTAAGCGAGTTCTTGAGAAGCTTTACAGGAAGCGGGACGACAAGGAGGATAACAGAAACAGAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0010467 GO:0016020 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

AAA_22 DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

729

Amino Acids

82.61

Weight (kDa)

6.98

Isoelectric Point (pI)

42.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	74 - 227	2.7e-10	DEAD/DEAH box helicase
AAA_22	PF13401	83 - 221	1.8e-07	AAA domain
Helicase_C	PF00271	268 - 407	8e-10	Helicase conserved C-terminal domain
WHD_HA2	PF04408	470 - 498	1.7e-11	Helicase associated domain (HA2), winged-helix
HA2_C	PF21010	499 - 532	6.3e-07	Helicase associated domain (HA2), ratchet-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008324)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47250 AT3G62310 AT3G62310 AT3G62310
fragaria_vesca	FvH4_1g26860 FvH4_1g26860
malus_domestica	MD09G1286000.v1.1 MD17G1278600.v1.1
prunus_persica	Prupe.3G007400_v2.0.a1
pyrus_communis	pycom09g19330 pycom17g27620 pycom17g27630
rosa_chinensis	RchiOBHm_Chr3g0487101
rosa_laevigata	RLG00000023033
rosa_multiflora	Rmu_co8377811.1_g000001 Rmu_ssc0000271.1_g000001
rosa_roxburghii	Rroxscaffold_6G00395180
rosa_rugosa	Rorug03G0231000
rosa_samantha	Rh3AG279300 Rh3BG315300 Rh3CG313500 Rh3DG310300
rosa_wichuraiana	Rw3G024760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1134
AarI	CACCTGC	1 cut(s)	1882
Acc16I	TGCGCA	1 cut(s)	1191
Acc36I	ACCTGC	1 cut(s)	1882
AccB1I	GGYRCC	3 cut(s)	101, 210, 946
AcoI	YGGCCR	1 cut(s)	2097
AcsI	RAATTY	2 cut(s)	753, 906
AcuI	CTGAAG	3 cut(s)	318, 432, 1038
AcyI	GRCGYC	1 cut(s)	102
AfaI	GTAC	9 cut(s)	7, 170, 364, 813, 1278, 1581, 1825, 1883, 1950
AfiI	CCNNNNNNNGG	4 cut(s)	220, 1603, 1795, 2116
AflIII	ACRYGT	1 cut(s)	1680
AjiI	CACGTC	1 cut(s)	40
AjnI	CCWGG	6 cut(s)	786, 835, 915, 1108, 1225, 1971
AluBI	AGCT	8 cut(s)	30, 320, 974, 1465, 1619, 1637, 1877, 2140
AluI	AGCT	8 cut(s)	30, 320, 974, 1465, 1619, 1637, 1877, 2140
Alw21I	GWGCWC	3 cut(s)	56, 715, 1411
Alw26I	GTCTC	4 cut(s)	266, 400, 824, 1388
Alw44I	GTGCAC	1 cut(s)	711
AlwNI	CAGNNNCTG	1 cut(s)	256
AoxI	GGCC	3 cut(s)	234, 1598, 2097
ApaLI	GTGCAC	1 cut(s)	711
ApeKI	GCWGC	7 cut(s)	65, 383, 471, 794, 979, 998, 1619
ApoI	RAATTY	2 cut(s)	753, 906
AseI	ATTAAT	1 cut(s)	1400
AspA2I	CCTAGG	1 cut(s)	1601
AspLEI	GCGC	2 cut(s)	104, 1192
AspS9I	GGNCC	5 cut(s)	144, 235, 932, 1025, 1378
AsuC2I	CCSGG	2 cut(s)	732, 2054
AsuHPI	GGTGA	6 cut(s)	37, 281, 932, 1481, 2005, 2069
AsuII	TTCGAA	1 cut(s)	802
AsuNHI	GCTAGC	1 cut(s)	1873
AvaII	GGWCC	4 cut(s)	144, 932, 1025, 1378
AvrII	CCTAGG	1 cut(s)	1601
BaeGI	GKGCMC	2 cut(s)	715, 951
BalI	TGGCCA	1 cut(s)	2099
BamHI	GGATCC	2 cut(s)	1495, 1711
BanI	GGYRCC	3 cut(s)	101, 210, 946
BauI	CACGAG	1 cut(s)	1140
BbsI	GAAGAC	3 cut(s)	200, 324, 471
Bbv12I	GWGCWC	3 cut(s)	56, 715, 1411
BbvI	GCAGC	7 cut(s)	52, 370, 483, 806, 985, 991, 1606
BccI	CCATC	8 cut(s)	346, 412, 500, 1079, 1442, 1649, 1723, 1969
BceAI	ACGGC	2 cut(s)	103, 387
BciT130I	CCWGG	6 cut(s)	788, 837, 917, 1110, 1227, 1973
BclI	TGATCA	2 cut(s)	132, 922
BcnI	CCSGG	2 cut(s)	732, 2054
BcoDI	GTCTC	4 cut(s)	266, 400, 824, 1388
BfaI	CTAG	6 cut(s)	1185, 1343, 1602, 1638, 1874, 2066
BfmI	CTRYAG	2 cut(s)	469, 1029
BfoI	RGCGCY	1 cut(s)	105
BfuAI	ACCTGC	1 cut(s)	1882
BglI	GCCNNNNNGGC	1 cut(s)	208
BlnI	CCTAGG	1 cut(s)	1601
BlpI	GCTNAGC	1 cut(s)	2121
BmcAI	AGTACT	1 cut(s)	1825
Bme1390I	CCNGG	8 cut(s)	732, 788, 837, 917, 1110, 1227, 1973, 2054
Bme18I	GGWCC	4 cut(s)	144, 932, 1025, 1378
BmgBI	CACGTC	1 cut(s)	40
BmgT120I	GGNCC	5 cut(s)	144, 235, 932, 1025, 1378
BmiI	GGNNCC	8 cut(s)	103, 212, 729, 771, 948, 1380, 1497, 1713
BmrFI	CCNGG	8 cut(s)	732, 788, 837, 917, 1110, 1227, 1973, 2054
BmrI	ACTGGG	1 cut(s)	2104
BmsI	GCATC	4 cut(s)	871, 1201, 1503, 1966
BmtI	GCTAGC	1 cut(s)	1877
BmuI	ACTGGG	1 cut(s)	2104
BpiI	GAAGAC	3 cut(s)	200, 324, 471
BplI	GAGNNNNNCTC	2 cut(s)	1650, 1682
BpmI	CTGGAG	4 cut(s)	809, 819, 954, 1374
Bpu1102I	GCTNAGC	1 cut(s)	2121
Bpu14I	TTCGAA	1 cut(s)	802
BpuEI	CTTGAG	6 cut(s)	203, 224, 698, 1433, 1796, 2153
BpuMI	CCSGG	2 cut(s)	732, 2054
Bsa29I	ATCGAT	1 cut(s)	1653
BsaBI	GATNNNNATC	1 cut(s)	1659
BsaHI	GRCGYC	1 cut(s)	102
BsaJI	CCNNGG	3 cut(s)	916, 1601, 2053
BsaWI	WCCGGW	2 cut(s)	146, 860
BsaXI	ACNNNNNCTCC	2 cut(s)	952, 982
Bsc4I	CCNNNNNNNGG	4 cut(s)	220, 1603, 1795, 2116
Bse1I	ACTGG	5 cut(s)	250, 280, 660, 1918, 2110
Bse3DI	GCAATG	3 cut(s)	393, 1059, 1823
Bse8I	GATNNNNATC	1 cut(s)	1659
BseBI	CCWGG	6 cut(s)	788, 837, 917, 1110, 1227, 1973
BseCI	ATCGAT	1 cut(s)	1653
BseDI	CCNNGG	3 cut(s)	916, 1601, 2053
BseGI	GGATG	4 cut(s)	427, 742, 1447, 1957
BseJI	GATNNNNATC	1 cut(s)	1659
BseLI	CCNNNNNNNGG	4 cut(s)	220, 1603, 1795, 2116
BseMI	GCAATG	3 cut(s)	393, 1059, 1823
BseMII	CTCAG	9 cut(s)	308, 675, 756, 765, 1235, 1242, 1292, 1514, 1521
BseNI	ACTGG	5 cut(s)	250, 280, 660, 1918, 2110
BseRI	GAGGAG	4 cut(s)	236, 884, 993, 996
BseSI	GKGCMC	2 cut(s)	715, 951
BseXI	GCAGC	7 cut(s)	52, 370, 483, 806, 985, 991, 1606
BseYI	CCCAGC	3 cut(s)	366, 1465, 1877
BshFI	GGCC	3 cut(s)	236, 1600, 2099
BshNI	GGYRCC	3 cut(s)	101, 210, 946
BshVI	ATCGAT	1 cut(s)	1653
BsiHKAI	GWGCWC	3 cut(s)	56, 715, 1411
BsiSI	CCGG	5 cut(s)	147, 732, 861, 1597, 2053
BslFI	GGGAC	3 cut(s)	794, 1856, 2168
BslI	CCNNNNNNNGG	4 cut(s)	220, 1603, 1795, 2116
BsmAI	GTCTC	4 cut(s)	266, 400, 824, 1388
BsmFI	GGGAC	3 cut(s)	794, 1856, 2168
BsmI	GAATGC	1 cut(s)	453
BsnI	GGCC	3 cut(s)	236, 1600, 2099
Bsp119I	TTCGAA	1 cut(s)	802
Bsp1286I	GDGCHC	4 cut(s)	56, 715, 951, 1411
Bsp1407I	TGTACA	2 cut(s)	811, 1948
Bsp1720I	GCTNAGC	1 cut(s)	2121
BspANI	GGCC	3 cut(s)	236, 1600, 2099
BspCNI	CTCAG	9 cut(s)	307, 676, 757, 766, 1234, 1243, 1293, 1513, 1522
BspDI	ATCGAT	1 cut(s)	1653
BspHI	TCATGA	1 cut(s)	580
BspLI	GGNNCC	8 cut(s)	103, 212, 729, 771, 948, 1380, 1497, 1713
BspMAI	CTGCAG	2 cut(s)	473, 1033
BspMI	ACCTGC	1 cut(s)	1882
BspOI	GCTAGC	1 cut(s)	1877
BspT104I	TTCGAA	1 cut(s)	802
BspT107I	GGYRCC	3 cut(s)	101, 210, 946
BsrDI	GCAATG	3 cut(s)	393, 1059, 1823
BsrGI	TGTACA	2 cut(s)	811, 1948
BsrI	ACTGG	5 cut(s)	250, 280, 660, 1918, 2110
BssECI	CCNNGG	3 cut(s)	916, 1601, 2053
BssNI	GRCGYC	1 cut(s)	102
BssSI	CACGAG	1 cut(s)	1140
BssT1I	CCWWGG	1 cut(s)	1601
Bst2BI	CACGAG	1 cut(s)	1140
Bst2UI	CCWGG	6 cut(s)	788, 837, 917, 1110, 1227, 1973
Bst4CI	ACNGT	7 cut(s)	58, 487, 557, 808, 1324, 1930, 2041
Bst6I	CTCTTC	6 cut(s)	11, 182, 408, 432, 861, 1250
BstACI	GRCGYC	1 cut(s)	102
BstAUI	TGTACA	2 cut(s)	811, 1948
BstBI	TTCGAA	1 cut(s)	802
BstC8I	GCNNGC	4 cut(s)	737, 886, 1209, 1875
BstF5I	GGATG	4 cut(s)	427, 742, 1447, 1957
BstH2I	RGCGCY	1 cut(s)	105
BstHHI	GCGC	2 cut(s)	104, 1192
BstMAI	GTCTC	4 cut(s)	266, 400, 824, 1388
BstMWI	GCNNNNNNNGC	7 cut(s)	96, 99, 160, 208, 1616, 1628, 1874
BstNI	CCWGG	6 cut(s)	788, 837, 917, 1110, 1227, 1973
BstNSI	RCATGY	1 cut(s)	888
BstSCI	CCNGG	8 cut(s)	730, 786, 835, 915, 1108, 1225, 1971, 2052
BstSFI	CTRYAG	2 cut(s)	469, 1029
BstSLI	GKGCMC	2 cut(s)	715, 951
BstV1I	GCAGC	7 cut(s)	52, 370, 483, 806, 985, 991, 1606
BstV2I	GAAGAC	3 cut(s)	200, 324, 471
BstX2I	RGATCY	5 cut(s)	177, 297, 535, 1495, 1711
BstYI	RGATCY	5 cut(s)	177, 297, 535, 1495, 1711
Bsu15I	ATCGAT	1 cut(s)	1653
BsuRI	GGCC	3 cut(s)	236, 1600, 2099
BsuTUI	ATCGAT	1 cut(s)	1653
BtgZI	GCGATG	1 cut(s)	542
BtrI	CACGTC	1 cut(s)	40
BtsCI	GGATG	4 cut(s)	427, 742, 1447, 1957
BtsI	GCAGTG	3 cut(s)	133, 481, 1670
BtsIMutI	CAGTG	7 cut(s)	133, 481, 562, 762, 1670, 1911, 2117
BveI	ACCTGC	1 cut(s)	1882
Cac8I	GCNNGC	4 cut(s)	737, 886, 1209, 1875
CaiI	CAGNNNCTG	1 cut(s)	256
CciI	TCATGA	1 cut(s)	580
CfoI	GCGC	2 cut(s)	104, 1192
Cfr13I	GGNCC	5 cut(s)	144, 235, 932, 1025, 1378
ClaI	ATCGAT	1 cut(s)	1653
CseI	GACGC	1 cut(s)	1004
Csp6I	GTAC	9 cut(s)	6, 169, 363, 812, 1277, 1580, 1824, 1882, 1949
CviAII	CATG	6 cut(s)	106, 581, 885, 1570, 1688, 1888
CviQI	GTAC	9 cut(s)	6, 169, 363, 812, 1277, 1580, 1824, 1882, 1949
DinI	GGCGCC	1 cut(s)	103
EaeI	YGGCCR	1 cut(s)	2097
Eam1104I	CTCTTC	6 cut(s)	11, 182, 408, 432, 861, 1250
EarI	CTCTTC	6 cut(s)	11, 182, 408, 432, 861, 1250
Eco130I	CCWWGG	1 cut(s)	1601
Eco32I	GATATC	1 cut(s)	2050
Eco47I	GGWCC	4 cut(s)	144, 932, 1025, 1378
Eco57I	CTGAAG	3 cut(s)	318, 432, 1038
EcoO109I	RGGNCCY	2 cut(s)	235, 1025
EcoRII	CCWGG	6 cut(s)	786, 835, 915, 1108, 1225, 1971
EcoRV	GATATC	1 cut(s)	2050
EcoT14I	CCWWGG	1 cut(s)	1601
EcoT22I	ATGCAT	2 cut(s)	886, 1693
EgeI	GGCGCC	1 cut(s)	103
EheI	GGCGCC	1 cut(s)	103
ErhI	CCWWGG	1 cut(s)	1601
FaeI	CATG	6 cut(s)	109, 584, 888, 1573, 1691, 1891
FalI	AAGNNNNNCTT	2 cut(s)	14, 46
FaqI	GGGAC	3 cut(s)	794, 1856, 2168
FatI	CATG	6 cut(s)	105, 580, 884, 1569, 1687, 1887
FauI	CCCGC	1 cut(s)	2145
FauNDI	CATATG	1 cut(s)	822
FbaI	TGATCA	2 cut(s)	132, 922
FokI	GGATG	4 cut(s)	434, 729, 1454, 1944
FspAI	RTGCGCAY	1 cut(s)	1191
FspBI	CTAG	6 cut(s)	1185, 1343, 1602, 1638, 1874, 2066
FspI	TGCGCA	1 cut(s)	1191
GlaI	GCGC	2 cut(s)	103, 1191
GsaI	CCCAGC	3 cut(s)	370, 1469, 1881
GsuI	CTGGAG	4 cut(s)	809, 819, 954, 1374
HaeII	RGCGCY	1 cut(s)	105
HaeIII	GGCC	3 cut(s)	236, 1600, 2099
HapII	CCGG	5 cut(s)	147, 732, 861, 1597, 2053
HgaI	GACGC	1 cut(s)	1004
HhaI	GCGC	2 cut(s)	104, 1192
Hin1I	GRCGYC	1 cut(s)	102
Hin1II	CATG	6 cut(s)	109, 584, 888, 1573, 1691, 1891
Hin6I	GCGC	2 cut(s)	102, 1190
HinP1I	GCGC	2 cut(s)	102, 1190
HincII	GTYRAC	1 cut(s)	1053
HindII	GTYRAC	1 cut(s)	1053
HindIII	AAGCTT	1 cut(s)	2138
HinfI	GANTC	5 cut(s)	109, 817, 832, 1154, 1837
HpaII	CCGG	5 cut(s)	147, 732, 861, 1597, 2053
HphI	GGTGA	6 cut(s)	37, 281, 932, 1481, 2005, 2069
Hpy166II	GTNNAC	4 cut(s)	46, 144, 713, 1053
Hpy188I	TCNGA	9 cut(s)	120, 297, 337, 501, 1197, 1241, 1503, 1746, 1862
Hpy188III	TCNNGA	9 cut(s)	241, 569, 581, 1292, 1391, 1493, 1841, 1904, 2132
Hpy8I	GTNNAC	4 cut(s)	46, 144, 713, 1053
Hpy99I	CGWCG	1 cut(s)	41
HpyAV	CCTTC	7 cut(s)	16, 211, 616, 718, 1013, 1029, 1927
HpyCH4III	ACNGT	7 cut(s)	58, 487, 557, 808, 1324, 1930, 2041
HpyCH4IV	ACGT	2 cut(s)	39, 1680
HpyF10VI	GCNNNNNNNGC	7 cut(s)	96, 99, 160, 208, 1616, 1628, 1874
HpySE526I	ACGT	2 cut(s)	39, 1680
Hsp92I	GRCGYC	1 cut(s)	102
Hsp92II	CATG	6 cut(s)	109, 584, 888, 1573, 1691, 1891
HspAI	GCGC	2 cut(s)	102, 1190
KasI	GGCGCC	1 cut(s)	101
Ksp22I	TGATCA	2 cut(s)	132, 922
LmnI	GCTCC	2 cut(s)	1006, 1393
Lsp1109I	GCAGC	7 cut(s)	52, 370, 483, 806, 985, 991, 1606
LweI	GCATC	4 cut(s)	871, 1201, 1503, 1966
MaeI	CTAG	6 cut(s)	1185, 1343, 1602, 1638, 1874, 2066
MaeII	ACGT	2 cut(s)	39, 1680
MaeIII	GTNAC	5 cut(s)	424, 698, 1451, 1916, 2041
MfeI	CAATTG	1 cut(s)	1585
MflI	RGATCY	5 cut(s)	177, 297, 535, 1495, 1711
MhlI	GDGCHC	4 cut(s)	56, 715, 951, 1411
MlsI	TGGCCA	1 cut(s)	2099
MluNI	TGGCCA	1 cut(s)	2099
Mly113I	GGCGCC	1 cut(s)	102
MlyI	GAGTC	2 cut(s)	826, 1846
MmeI	TCCRAC	2 cut(s)	525, 1264
Mox20I	TGGCCA	1 cut(s)	2099
Mph1103I	ATGCAT	2 cut(s)	886, 1693
MscI	TGGCCA	1 cut(s)	2099
MseI	TTAA	5 cut(s)	518, 1400, 1806, 1833, 2015
MslI	CAYNNNNRTG	5 cut(s)	825, 1653, 1720, 1892, 2088
Msp20I	TGGCCA	1 cut(s)	2099
MspI	CCGG	5 cut(s)	147, 732, 861, 1597, 2053
MspR9I	CCNGG	8 cut(s)	732, 788, 837, 917, 1110, 1227, 1973, 2054
MunI	CAATTG	1 cut(s)	1585
Mva1269I	GAATGC	1 cut(s)	453
MvaI	CCWGG	6 cut(s)	788, 837, 917, 1110, 1227, 1973
MwoI	GCNNNNNNNGC	7 cut(s)	96, 99, 160, 208, 1616, 1628, 1874
NarI	GGCGCC	1 cut(s)	102
NciI	CCSGG	2 cut(s)	732, 2054
NdeI	CATATG	1 cut(s)	822
NheI	GCTAGC	1 cut(s)	1873
NlaIII	CATG	6 cut(s)	109, 584, 888, 1573, 1691, 1891
NlaIV	GGNNCC	8 cut(s)	103, 212, 729, 771, 948, 1380, 1497, 1713
NmuCI	GTSAC	1 cut(s)	1916
NsbI	TGCGCA	1 cut(s)	1191
NsiI	ATGCAT	2 cut(s)	886, 1693
NspI	RCATGY	1 cut(s)	888
NspV	TTCGAA	1 cut(s)	802
PaeI	GCATGC	1 cut(s)	888
PagI	TCATGA	1 cut(s)	580
PaqCI	CACCTGC	1 cut(s)	1882
PcsI	WCGNNNNNNNCGW	1 cut(s)	45
PctI	GAATGC	1 cut(s)	453
PfeI	GAWTC	3 cut(s)	109, 817, 1154
PfoI	TCCNGGA	1 cut(s)	835
PleI	GAGTC	2 cut(s)	826, 1845
PluTI	GGCGCC	1 cut(s)	105
PpsI	GAGTC	2 cut(s)	826, 1845
PpuMI	RGGWCCY	1 cut(s)	1025
PshBI	ATTAAT	1 cut(s)	1400
PsiI	TTATAA	1 cut(s)	1134
Psp5II	RGGWCCY	1 cut(s)	1025
Psp6I	CCWGG	6 cut(s)	786, 835, 915, 1108, 1225, 1971
PspFI	CCCAGC	3 cut(s)	366, 1465, 1877
PspGI	CCWGG	6 cut(s)	786, 835, 915, 1108, 1225, 1971
PspN4I	GGNNCC	8 cut(s)	103, 212, 729, 771, 948, 1380, 1497, 1713
PspPI	GGNCC	5 cut(s)	144, 235, 932, 1025, 1378
PspPPI	RGGWCCY	1 cut(s)	1025
PstI	CTGCAG	2 cut(s)	473, 1033
PstNI	CAGNNNCTG	1 cut(s)	256
PsuI	RGATCY	5 cut(s)	177, 297, 535, 1495, 1711
RsaI	GTAC	9 cut(s)	7, 170, 364, 813, 1278, 1581, 1825, 1883, 1950
RsaNI	GTAC	9 cut(s)	6, 169, 363, 812, 1277, 1580, 1824, 1882, 1949
RseI	CAYNNNNRTG	5 cut(s)	825, 1653, 1720, 1892, 2088
SaqAI	TTAA	5 cut(s)	518, 1400, 1806, 1833, 2015
Sau96I	GGNCC	5 cut(s)	144, 235, 932, 1025, 1378
SbfI	CCTGCAGG	1 cut(s)	1033
ScaI	AGTACT	1 cut(s)	1825
SchI	GAGTC	2 cut(s)	826, 1846
ScrFI	CCNGG	8 cut(s)	732, 788, 837, 917, 1110, 1227, 1973, 2054
SdaI	CCTGCAGG	1 cut(s)	1033
SduI	GDGCHC	4 cut(s)	56, 715, 951, 1411
SfaNI	GCATC	4 cut(s)	871, 1201, 1503, 1966
SfcI	CTRYAG	2 cut(s)	469, 1029
SfoI	GGCGCC	1 cut(s)	103
SfuI	TTCGAA	1 cut(s)	802
SinI	GGWCC	4 cut(s)	144, 932, 1025, 1378
SmiMI	CAYNNNNRTG	5 cut(s)	825, 1653, 1720, 1892, 2088
SmlI	CTYRAG	6 cut(s)	182, 239, 677, 1412, 1811, 2132
SmoI	CTYRAG	6 cut(s)	182, 239, 677, 1412, 1811, 2132
SphI	GCATGC	1 cut(s)	888
Sse8387I	CCTGCAGG	1 cut(s)	1033
SspDI	GGCGCC	1 cut(s)	101
SspI	AATATT	1 cut(s)	990
SspMI	CTAG	6 cut(s)	1185, 1343, 1602, 1638, 1874, 2066
StyD4I	CCNGG	8 cut(s)	730, 786, 835, 915, 1108, 1225, 1971, 2052
StyI	CCWWGG	1 cut(s)	1601
TaaI	ACNGT	7 cut(s)	58, 487, 557, 808, 1324, 1930, 2041
TaiI	ACGT	2 cut(s)	42, 1683
TaqI	TCGA	4 cut(s)	36, 802, 1559, 1653
TatI	WGTACW	3 cut(s)	811, 1823, 1948
TauI	GCSGC	6 cut(s)	65, 93, 96, 99, 102, 891
TfiI	GAWTC	3 cut(s)	109, 817, 1154
Tru1I	TTAA	5 cut(s)	518, 1400, 1806, 1833, 2015
Tru9I	TTAA	5 cut(s)	518, 1400, 1806, 1833, 2015
TscAI	CASTG	7 cut(s)	133, 481, 562, 769, 1677, 1918, 2117
TseFI	GTSAC	1 cut(s)	1916
TseI	GCWGC	7 cut(s)	65, 383, 471, 794, 979, 998, 1619
Tsp45I	GTSAC	1 cut(s)	1916
TspDTI	ATGAA	8 cut(s)	89, 122, 731, 737, 809, 1253, 1352, 1876
TspGWI	ACGGA	1 cut(s)	318
TspRI	CASTG	7 cut(s)	133, 481, 562, 769, 1677, 1918, 2117
VneI	GTGCAC	1 cut(s)	711
VpaK11BI	GGWCC	4 cut(s)	144, 932, 1025, 1378
VspI	ATTAAT	1 cut(s)	1400
XapI	RAATTY	2 cut(s)	753, 906
XceI	RCATGY	1 cut(s)	888
XmaJI	CCTAGG	1 cut(s)	1601
XspI	CTAG	6 cut(s)	1185, 1343, 1602, 1638, 1874, 2066
ZrmI	AGTACT	1 cut(s)	1825
Zsp2I	ATGCAT	2 cut(s)	886, 1693

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.