Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0008324

Physical Location & Seq

Genomic Coordinates Forward (+)

16991836 .. 16995545

Gene Structure 3710 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000023033

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2190 bp

ATGGGTACGGAGAGGAAGAGGAAGGTGAGCTTGTTCGACGTGGTGGACGAGACCACTGTTGCAGCTGCGAAGATGAGCAAATCGAACGGTGGTGGAGGCGCCATGAATCACAACAACCCGATGAGCAGTCTGATCAATAGGTGGACCGGAAAGCCCTACTCGCAAAGGTACTATGAGATCCTTGAGAAGAGGAAGACTTTGCCTGTTTGGCACCAGAAGGAGGAGTTCTTTCAGGTTCTCAAGAAAAGTCAGTCGCTGATTCTCGTTGGTGAGACTGGTAGTGGTAAAACCACTCAGATTCCTCAATTTGTTTTGGAAGCTGTTGATAGGGAGTCTTCAGATAAAAGCAGGAAGATGATTGCATGTACCCAGCCTCGTAGGGTGGCTGCAATGTCTGTGTCTCGCCGTGTTGCTGAAGAGATGGATGTTACCATTGGGGAAGAGGTTGGTTACAGCATTCGTTTTGAAGACTGCAGCAGTGCCAGAACAGTATTGAAGTATCTAACAGATGGTATGCTTTTAAGAGAAGCCATGACAGATCCACTGTTAGAACGATACAGTGTAATAATTCTTGATGAGGCTCATGAGAGGACTTTAGCAACAGATGTTCTGTTTGGGCTTCTGAAGGAAGTATTGAAAAATAGACCTGATATGAAGCTGGTTGTTATGAGTGCTACACTTGAGGCTGAGAAGTTTCAGGGTTATTTCAACGGTGCACCACTAATGAAGGTCCCTGGCAGGCTTCATCCAGTTGAAATTTTTTACACTGAGGAACCTGAGAGGGACTACGTGGAGGCAGCAATTCGAACTGTTGTACAGATTCATATGTATGAGACTCCAGGAGATATACTTGTTTTTTTAACTGGAGAAGAGGAGATAGAAGATGCATGCCGCAAAATCAACAAAGAAATTTCAAACCTGGGTGATCAGGTTGGTCCAGTGAAAGTGGTGCCCCTGTATTCAACTCTCCCTCCAGCTATGCAGCAGAAAATATTTGACGCTGCCCCTCCTCCCGCAACAGAAGGTGGTCCTGCAGGGAGGAAAATTGTGGTGTCAACTAACATCGCAGAAACTTCTCTGACCATAGATGGGATCGTGTATGTTATTGATCCAGGTTTTTCTAAACAGAAAGTTTATAACCCACGAGTGCGTGTTGAATCCTTATTGGTATCTCCGATTTCTAAGGCTAGTGCACATCAGAGGTCAGGGCGTGCTGGAAGAACTCAGCCAGGCAAATGTTTCAGACTTTATACTGAGAAAAGTTTCCATAATGATCTTGTACCGCAGACATATCCAGAAATACTGAGATCAAATCTTGCAAATACAGTGCTTACTTTGAAGAAACTAGGAATAGATGATTTGGTTCATTTTGATTTTATGGATCCCCCTGCTCCAGAGACATTAATGAGAGCACTTGAGGTTTTGAATTATTTGGGTGCATTGGATGATGATGGTAACTTGACCAAGCTGGGTGAGATTATGAGCGAGTTCCCTCTGGATCCTCAGATGTCAAAGATGCTTGTTGTTAGCCCCGAGTTTAACTGCTCAAATGAGATTTTGTCGATTTCTGCCATGCTTTCAGTACCCAATTGCTTTGTCCGGCCTAGGGAGGCTCAGAAAGCTGCAGATGAAGCAAAAGCTAGGTTTGGACACATCGATGGAGATCATCTCACACTGCTGAATGTGTATCATGCATACAAGCAAAACAATGAAGATCCATCGTGGTGCTATGAGAACTTTGTCAATCAGAGGGCATTGAAGTCTGCAGACAATGTTAGACAACAGCTAGTGCGCATTATGGCTCGGTTTAATCTCAAGCTATGCAGTACTGATTTTAAGAGTCGGGACTACTACATCAACATCAGAAAGGCTATGCTAGCTGGGTACTTCATGCAGGTGGCTCATCTGGAACGCACTGGTCACTACCTTACTGTGAAGGATAACCAAGTTGTACATTTGCATCCATCAAATTGCTTGGATCACAAGCCAGAGTGGGTGATTTACAATGAGTACGTCTTAACGAGCAGGAATTTTATCCGCACAGTGACAGATATCCGTGGTGAATGGCTAGTTGATATAGCACCACACTACTATGATTTGGCCAACTTTCCCCAGTGTGAGGCCAAGCGAGTTCTTGAGAAGCTTTACAGGAAGCGGGATGACAAGGAGGATAACAGAAACAGAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0010467 GO:0016020 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

AAA_22 DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

730

Amino Acids

82.84

Weight (kDa)

7.0

Isoelectric Point (pI)

43.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	73 - 228	6.1e-10	DEAD/DEAH box helicase
AAA_22	PF13401	83 - 213	1.6e-07	AAA domain
Helicase_C	PF00271	269 - 408	1.3e-09	Helicase conserved C-terminal domain
WHD_HA2	PF04408	471 - 499	2.3e-11	Helicase associated domain (HA2), winged-helix
HA2_C	PF21010	500 - 559	1.1e-14	Helicase associated domain (HA2), ratchet-like
OB_NTP_bind	PF07717	621 - 697	8.6e-25	Oligonucleotide/oligosaccharide-binding (OB)-fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008324)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47250 AT3G62310 AT3G62310 AT3G62310
fragaria_vesca	FvH4_1g26860 FvH4_1g26860
malus_domestica	MD09G1286000.v1.1 MD17G1278600.v1.1
prunus_persica	Prupe.3G007400_v2.0.a1
pyrus_communis	pycom09g19330 pycom17g27620 pycom17g27630
rosa_chinensis	RchiOBHm_Chr3g0487101
rosa_laevigata	RLG00000023033
rosa_multiflora	Rmu_co8377811.1_g000001 Rmu_ssc0000271.1_g000001
rosa_roxburghii	Rroxscaffold_6G00395180
rosa_rugosa	Rorug03G0231000
rosa_samantha	Rh3AG279300 Rh3BG315300 Rh3CG313500 Rh3DG310300
rosa_wichuraiana	Rw3G024760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1137
AarI	CACCTGC	1 cut(s)	1885
Acc16I	TGCGCA	1 cut(s)	1793
Acc36I	ACCTGC	1 cut(s)	1885
AccB1I	GGYRCC	3 cut(s)	98, 210, 949
AciI	CCGC	5 cut(s)	892, 1014, 1283, 2038, 2155
AcoI	YGGCCR	1 cut(s)	2100
AcsI	RAATTY	3 cut(s)	756, 909, 2029
AcuI	CTGAAG	3 cut(s)	321, 435, 644
AcyI	GRCGYC	1 cut(s)	99
AfiI	CCNNNNNNNGG	4 cut(s)	220, 1089, 1606, 2119
AjiI	CACGTC	1 cut(s)	40
AjnI	CCWGG	5 cut(s)	733, 838, 918, 1111, 1228
Alw21I	GWGCWC	3 cut(s)	718, 1195, 1414
Alw26I	GTCTC	5 cut(s)	44, 266, 405, 827, 1391
Alw44I	GTGCAC	2 cut(s)	714, 1191
AlwNI	CAGNNNCTG	1 cut(s)	256
Ama87I	CYCGRG	1 cut(s)	1532
AoxI	GGCC	3 cut(s)	1601, 2100, 2121
ApaLI	GTGCAC	2 cut(s)	714, 1191
ApeKI	GCWGC	8 cut(s)	62, 65, 386, 474, 797, 982, 1001, 1622
ApoI	RAATTY	3 cut(s)	756, 909, 2029
AseI	ATTAAT	1 cut(s)	1403
Asp700I	GAANNNNTTC	1 cut(s)	1262
AspA2I	CCTAGG	1 cut(s)	1604
AspLEI	GCGC	2 cut(s)	101, 1794
AspS9I	GGNCC	4 cut(s)	144, 730, 935, 1028
AsuHPI	GGTGA	6 cut(s)	37, 281, 935, 1484, 2008, 2072
AsuII	TTCGAA	1 cut(s)	805
AsuNHI	GCTAGC	1 cut(s)	1876
AvaI	CYCGRG	1 cut(s)	1532
AvaII	GGWCC	4 cut(s)	144, 730, 935, 1028
AvrII	CCTAGG	1 cut(s)	1604
BaeGI	GKGCMC	3 cut(s)	718, 954, 1195
BalI	TGGCCA	1 cut(s)	2102
BamHI	GGATCC	2 cut(s)	1381, 1498
BanI	GGYRCC	3 cut(s)	98, 210, 949
BauI	CACGAG	1 cut(s)	1143
BbsI	GAAGAC	3 cut(s)	200, 327, 474
Bbv12I	GWGCWC	3 cut(s)	718, 1195, 1414
BbvI	GCAGC	8 cut(s)	52, 74, 373, 486, 809, 988, 994, 1609
BccI	CCATC	7 cut(s)	415, 503, 1082, 1445, 1652, 1726, 1972
BceAI	ACGGC	1 cut(s)	390
BciT130I	CCWGG	5 cut(s)	735, 840, 920, 1113, 1230
BclI	TGATCA	2 cut(s)	132, 925
BcoDI	GTCTC	5 cut(s)	44, 266, 405, 827, 1391
BfaI	CTAG	7 cut(s)	1188, 1346, 1605, 1641, 1787, 1877, 2069
BfmI	CTRYAG	4 cut(s)	472, 1032, 1623, 1764
BfoI	RGCGCY	1 cut(s)	102
BfuAI	ACCTGC	1 cut(s)	1885
BglI	GCCNNNNNGGC	1 cut(s)	208
BisI	GCNGC	9 cut(s)	63, 66, 387, 475, 798, 892, 983, 1002, 1623
BlnI	CCTAGG	1 cut(s)	1604
BlsI	GCNGC	9 cut(s)	64, 67, 388, 476, 799, 893, 984, 1003, 1624
BmcAI	AGTACT	1 cut(s)	1828
Bme1390I	CCNGG	5 cut(s)	735, 840, 920, 1113, 1230
Bme18I	GGWCC	4 cut(s)	144, 730, 935, 1028
BmeT110I	CYCGRG	1 cut(s)	1532
BmgBI	CACGTC	1 cut(s)	40
BmgT120I	GGNCC	4 cut(s)	144, 730, 935, 1028
BmiI	GGNNCC	7 cut(s)	100, 212, 732, 774, 951, 1383, 1500
BmrFI	CCNGG	5 cut(s)	735, 840, 920, 1113, 1230
BmrI	ACTGGG	1 cut(s)	2107
BmsI	GCATC	3 cut(s)	874, 1506, 1969
BmtI	GCTAGC	1 cut(s)	1880
BmuI	ACTGGG	1 cut(s)	2107
BpiI	GAAGAC	3 cut(s)	200, 327, 474
BplI	GAGNNNNNCTC	2 cut(s)	1653, 1685
BpmI	CTGGAG	4 cut(s)	822, 885, 957, 1377
Bpu14I	TTCGAA	1 cut(s)	805
BpuEI	CTTGAG	6 cut(s)	203, 224, 701, 1436, 1799, 2156
Bsa29I	ATCGAT	1 cut(s)	1656
BsaAI	YACGTR	1 cut(s)	790
BsaBI	GATNNNNATC	1 cut(s)	1662
BsaHI	GRCGYC	1 cut(s)	99
BsaI	GGTCTC	1 cut(s)	44
BsaJI	CCNNGG	4 cut(s)	733, 919, 1604, 2056
BsaWI	WCCGGW	1 cut(s)	146
BsaXI	ACNNNNNCTCC	2 cut(s)	955, 985
Bsc4I	CCNNNNNNNGG	4 cut(s)	220, 1089, 1606, 2119
Bse1I	ACTGG	6 cut(s)	280, 749, 868, 938, 1921, 2113
Bse3DI	GCAATG	1 cut(s)	396
Bse8I	GATNNNNATC	1 cut(s)	1662
BseBI	CCWGG	5 cut(s)	735, 840, 920, 1113, 1230
BseCI	ATCGAT	1 cut(s)	1656
BseDI	CCNNGG	4 cut(s)	733, 919, 1604, 2056
BseGI	GGATG	5 cut(s)	430, 745, 1450, 1960, 2164
BseJI	GATNNNNATC	1 cut(s)	1662
BseLI	CCNNNNNNNGG	4 cut(s)	220, 1089, 1606, 2119
BseMI	GCAATG	1 cut(s)	396
BseMII	CTCAG	9 cut(s)	308, 678, 759, 768, 1238, 1245, 1295, 1517, 1628
BseNI	ACTGG	6 cut(s)	280, 749, 868, 938, 1921, 2113
BseRI	GAGGAG	3 cut(s)	236, 887, 999
BseSI	GKGCMC	3 cut(s)	718, 954, 1195
BseXI	GCAGC	8 cut(s)	52, 74, 373, 486, 809, 988, 994, 1609
BseYI	CCCAGC	3 cut(s)	369, 1468, 1880
BshFI	GGCC	3 cut(s)	1603, 2102, 2123
BshNI	GGYRCC	3 cut(s)	98, 210, 949
BshVI	ATCGAT	1 cut(s)	1656
BsiHKAI	GWGCWC	3 cut(s)	718, 1195, 1414
BsiHKCI	CYCGRG	1 cut(s)	1532
BsiSI	CCGG	2 cut(s)	147, 1600
BslFI	GGGAC	3 cut(s)	716, 797, 1859
BslI	CCNNNNNNNGG	4 cut(s)	220, 1089, 1606, 2119
BsmAI	GTCTC	5 cut(s)	44, 266, 405, 827, 1391
BsmFI	GGGAC	3 cut(s)	716, 797, 1859
BsmI	GAATGC	1 cut(s)	456
BsnI	GGCC	3 cut(s)	1603, 2102, 2123
Bso31I	GGTCTC	1 cut(s)	44
BsoBI	CYCGRG	1 cut(s)	1532
Bsp119I	TTCGAA	1 cut(s)	805
Bsp1286I	GDGCHC	4 cut(s)	718, 954, 1195, 1414
Bsp1407I	TGTACA	2 cut(s)	814, 1951
BspACI	CCGC	5 cut(s)	892, 1014, 1283, 2038, 2155
BspANI	GGCC	3 cut(s)	1603, 2102, 2123
BspCNI	CTCAG	9 cut(s)	307, 679, 760, 769, 1237, 1246, 1296, 1516, 1627
BspDI	ATCGAT	1 cut(s)	1656
BspHI	TCATGA	1 cut(s)	583
BspLI	GGNNCC	7 cut(s)	100, 212, 732, 774, 951, 1383, 1500
BspMAI	CTGCAG	4 cut(s)	476, 1036, 1627, 1768
BspMI	ACCTGC	1 cut(s)	1885
BspOI	GCTAGC	1 cut(s)	1880
BspT104I	TTCGAA	1 cut(s)	805
BspT107I	GGYRCC	3 cut(s)	98, 210, 949
BspTNI	GGTCTC	1 cut(s)	44
BsrDI	GCAATG	1 cut(s)	396
BsrGI	TGTACA	2 cut(s)	814, 1951
BsrI	ACTGG	6 cut(s)	280, 749, 868, 938, 1921, 2113
BssECI	CCNNGG	4 cut(s)	733, 919, 1604, 2056
BssNI	GRCGYC	1 cut(s)	99
BssSI	CACGAG	1 cut(s)	1143
BssT1I	CCWWGG	1 cut(s)	1604
Bst2BI	CACGAG	1 cut(s)	1143
Bst2UI	CCWGG	5 cut(s)	735, 840, 920, 1113, 1230
Bst6I	CTCTTC	5 cut(s)	11, 182, 411, 435, 864
BstACI	GRCGYC	1 cut(s)	99
BstAUI	TGTACA	2 cut(s)	814, 1951
BstBAI	YACGTR	1 cut(s)	790
BstBI	TTCGAA	1 cut(s)	805
BstC8I	GCNNGC	4 cut(s)	740, 889, 1212, 1878
BstDSI	CCRYGG	1 cut(s)	2056
BstF5I	GGATG	5 cut(s)	430, 745, 1450, 1960, 2164
BstH2I	RGCGCY	1 cut(s)	102
BstHHI	GCGC	2 cut(s)	101, 1794
BstMAI	GTCTC	5 cut(s)	44, 266, 405, 827, 1391
BstMWI	GCNNNNNNNGC	5 cut(s)	160, 208, 1619, 1631, 1877
BstNI	CCWGG	5 cut(s)	735, 840, 920, 1113, 1230
BstNSI	RCATGY	2 cut(s)	366, 891
BstSCI	CCNGG	5 cut(s)	733, 838, 918, 1111, 1228
BstSFI	CTRYAG	4 cut(s)	472, 1032, 1623, 1764
BstSLI	GKGCMC	3 cut(s)	718, 954, 1195
BstV1I	GCAGC	8 cut(s)	52, 74, 373, 486, 809, 988, 994, 1609
BstV2I	GAAGAC	3 cut(s)	200, 327, 474
BstX2I	RGATCY	5 cut(s)	177, 538, 1381, 1498, 1714
BstYI	RGATCY	5 cut(s)	177, 538, 1381, 1498, 1714
Bsu15I	ATCGAT	1 cut(s)	1656
BsuRI	GGCC	3 cut(s)	1603, 2102, 2123
BsuTUI	ATCGAT	1 cut(s)	1656
BtgI	CCRYGG	1 cut(s)	2056
BtgZI	GCGATG	1 cut(s)	1048
BtrI	CACGTC	1 cut(s)	40
BtsCI	GGATG	5 cut(s)	430, 745, 1450, 1960, 2164
BtsI	GCAGTG	2 cut(s)	484, 1673
BveI	ACCTGC	1 cut(s)	1885
Cac8I	GCNNGC	4 cut(s)	740, 889, 1212, 1878
CaiI	CAGNNNCTG	1 cut(s)	256
CciI	TCATGA	1 cut(s)	583
CfoI	GCGC	2 cut(s)	101, 1794
Cfr13I	GGNCC	4 cut(s)	144, 730, 935, 1028
ClaI	ATCGAT	1 cut(s)	1656
CseI	GACGC	1 cut(s)	1007
CviAII	CATG	8 cut(s)	103, 363, 532, 584, 888, 1573, 1691, 1891
DinI	GGCGCC	1 cut(s)	100
EaeI	YGGCCR	1 cut(s)	2100
Eam1104I	CTCTTC	5 cut(s)	11, 182, 411, 435, 864
EarI	CTCTTC	5 cut(s)	11, 182, 411, 435, 864
Eco130I	CCWWGG	1 cut(s)	1604
Eco31I	GGTCTC	1 cut(s)	44
Eco32I	GATATC	1 cut(s)	2053
Eco47I	GGWCC	4 cut(s)	144, 730, 935, 1028
Eco57I	CTGAAG	3 cut(s)	321, 435, 644
Eco88I	CYCGRG	1 cut(s)	1532
EcoO109I	RGGNCCY	1 cut(s)	730
EcoRII	CCWGG	5 cut(s)	733, 838, 918, 1111, 1228
EcoRV	GATATC	1 cut(s)	2053
EcoT14I	CCWWGG	1 cut(s)	1604
EcoT22I	ATGCAT	2 cut(s)	889, 1696
EgeI	GGCGCC	1 cut(s)	100
EheI	GGCGCC	1 cut(s)	100
ErhI	CCWWGG	1 cut(s)	1604
FaeI	CATG	8 cut(s)	106, 366, 535, 587, 891, 1576, 1694, 1894
FalI	AAGNNNNNCTT	2 cut(s)	14, 46
FaqI	GGGAC	3 cut(s)	716, 797, 1859
FatI	CATG	8 cut(s)	102, 362, 531, 583, 887, 1572, 1690, 1890
FauI	CCCGC	2 cut(s)	1021, 2148
FauNDI	CATATG	1 cut(s)	825
FbaI	TGATCA	2 cut(s)	132, 925
Fnu4HI	GCNGC	9 cut(s)	63, 66, 387, 475, 798, 892, 983, 1002, 1623
FokI	GGATG	5 cut(s)	437, 732, 1457, 1947, 2171
Fsp4HI	GCNGC	9 cut(s)	63, 66, 387, 475, 798, 892, 983, 1002, 1623
FspAI	RTGCGCAY	1 cut(s)	1793
FspBI	CTAG	7 cut(s)	1188, 1346, 1605, 1641, 1787, 1877, 2069
FspI	TGCGCA	1 cut(s)	1793
GlaI	GCGC	2 cut(s)	100, 1793
GluI	GCNGC	9 cut(s)	63, 66, 387, 475, 798, 892, 983, 1002, 1623
GsaI	CCCAGC	3 cut(s)	373, 1472, 1884
GsuI	CTGGAG	4 cut(s)	822, 885, 957, 1377
HaeII	RGCGCY	1 cut(s)	102
HaeIII	GGCC	3 cut(s)	1603, 2102, 2123
HapII	CCGG	2 cut(s)	147, 1600
HgaI	GACGC	1 cut(s)	1007
HhaI	GCGC	2 cut(s)	101, 1794
Hin1I	GRCGYC	1 cut(s)	99
Hin1II	CATG	8 cut(s)	106, 366, 535, 587, 891, 1576, 1694, 1894
Hin6I	GCGC	2 cut(s)	99, 1792
HinP1I	GCGC	2 cut(s)	99, 1792
HincII	GTYRAC	1 cut(s)	1056
HindII	GTYRAC	1 cut(s)	1056
HindIII	AAGCTT	1 cut(s)	2141
HinfI	GANTC	8 cut(s)	106, 259, 298, 332, 820, 835, 1157, 1840
HpaII	CCGG	2 cut(s)	147, 1600
HphI	GGTGA	6 cut(s)	37, 281, 935, 1484, 2008, 2072
Hpy166II	GTNNAC	5 cut(s)	46, 144, 716, 1056, 1193
Hpy188III	TCNNGA	9 cut(s)	241, 572, 584, 1295, 1394, 1496, 1844, 1907, 2135
Hpy8I	GTNNAC	5 cut(s)	46, 144, 716, 1056, 1193
Hpy99I	CGWCG	1 cut(s)	41
HpyAV	CCTTC	6 cut(s)	16, 211, 619, 721, 1016, 1930
HpyCH4IV	ACGT	3 cut(s)	39, 789, 2013
HpyF10VI	GCNNNNNNNGC	5 cut(s)	160, 208, 1619, 1631, 1877
HpySE526I	ACGT	3 cut(s)	39, 789, 2013
Hsp92I	GRCGYC	1 cut(s)	99
Hsp92II	CATG	8 cut(s)	106, 366, 535, 587, 891, 1576, 1694, 1894
HspAI	GCGC	2 cut(s)	99, 1792
KasI	GGCGCC	1 cut(s)	98
Ksp22I	TGATCA	2 cut(s)	132, 925
LmnI	GCTCC	1 cut(s)	1396
Lsp1109I	GCAGC	8 cut(s)	52, 74, 373, 486, 809, 988, 994, 1609
LweI	GCATC	3 cut(s)	874, 1506, 1969
MaeI	CTAG	7 cut(s)	1188, 1346, 1605, 1641, 1787, 1877, 2069
MaeII	ACGT	3 cut(s)	39, 789, 2013
MaeIII	GTNAC	5 cut(s)	427, 449, 1454, 1919, 2044
MfeI	CAATTG	1 cut(s)	1588
MflI	RGATCY	5 cut(s)	177, 538, 1381, 1498, 1714
MhlI	GDGCHC	4 cut(s)	718, 954, 1195, 1414
MlsI	TGGCCA	1 cut(s)	2102
MluNI	TGGCCA	1 cut(s)	2102
Mly113I	GGCGCC	1 cut(s)	99
MlyI	GAGTC	3 cut(s)	341, 829, 1849
Mox20I	TGGCCA	1 cut(s)	2102
Mph1103I	ATGCAT	2 cut(s)	889, 1696
MroXI	GAANNNNTTC	1 cut(s)	1262
MscI	TGGCCA	1 cut(s)	2102
MseI	TTAA	7 cut(s)	521, 860, 1403, 1539, 1809, 1836, 2018
MslI	CAYNNNNRTG	5 cut(s)	828, 1656, 1723, 1895, 2091
Msp20I	TGGCCA	1 cut(s)	2102
MspA1I	CMGCKG	1 cut(s)	65
MspI	CCGG	2 cut(s)	147, 1600
MspR9I	CCNGG	5 cut(s)	735, 840, 920, 1113, 1230
MunI	CAATTG	1 cut(s)	1588
Mva1269I	GAATGC	1 cut(s)	456
MvaI	CCWGG	5 cut(s)	735, 840, 920, 1113, 1230
MwoI	GCNNNNNNNGC	5 cut(s)	160, 208, 1619, 1631, 1877
NarI	GGCGCC	1 cut(s)	99
NdeI	CATATG	1 cut(s)	825
NheI	GCTAGC	1 cut(s)	1876
NlaIII	CATG	8 cut(s)	106, 366, 535, 587, 891, 1576, 1694, 1894
NlaIV	GGNNCC	7 cut(s)	100, 212, 732, 774, 951, 1383, 1500
NmuCI	GTSAC	2 cut(s)	1919, 2044
NsbI	TGCGCA	1 cut(s)	1793
NsiI	ATGCAT	2 cut(s)	889, 1696
NspI	RCATGY	2 cut(s)	366, 891
NspV	TTCGAA	1 cut(s)	805
PaeI	GCATGC	1 cut(s)	891
PagI	TCATGA	1 cut(s)	583
PaqCI	CACCTGC	1 cut(s)	1885
PcsI	WCGNNNNNNNCGW	1 cut(s)	45
PctI	GAATGC	1 cut(s)	456
PdmI	GAANNNNTTC	1 cut(s)	1262
PfeI	GAWTC	5 cut(s)	106, 259, 298, 820, 1157
PfoI	TCCNGGA	1 cut(s)	838
PkrI	GCNGC	9 cut(s)	64, 67, 388, 476, 799, 893, 984, 1003, 1624
PleI	GAGTC	3 cut(s)	340, 829, 1848
PluTI	GGCGCC	1 cut(s)	102
PpsI	GAGTC	3 cut(s)	340, 829, 1848
Ppu21I	YACGTR	1 cut(s)	790
PpuMI	RGGWCCY	1 cut(s)	730
PshBI	ATTAAT	1 cut(s)	1403
PsiI	TTATAA	1 cut(s)	1137
Psp5II	RGGWCCY	1 cut(s)	730
Psp6I	CCWGG	5 cut(s)	733, 838, 918, 1111, 1228
PspFI	CCCAGC	3 cut(s)	369, 1468, 1880
PspGI	CCWGG	5 cut(s)	733, 838, 918, 1111, 1228
PspN4I	GGNNCC	7 cut(s)	100, 212, 732, 774, 951, 1383, 1500
PspPI	GGNCC	4 cut(s)	144, 730, 935, 1028
PspPPI	RGGWCCY	1 cut(s)	730
PstI	CTGCAG	4 cut(s)	476, 1036, 1627, 1768
PstNI	CAGNNNCTG	1 cut(s)	256
PsuI	RGATCY	5 cut(s)	177, 538, 1381, 1498, 1714
PvuII	CAGCTG	1 cut(s)	65
RseI	CAYNNNNRTG	5 cut(s)	828, 1656, 1723, 1895, 2091
SaqAI	TTAA	7 cut(s)	521, 860, 1403, 1539, 1809, 1836, 2018
SatI	GCNGC	9 cut(s)	63, 66, 387, 475, 798, 892, 983, 1002, 1623
Sau96I	GGNCC	4 cut(s)	144, 730, 935, 1028
SbfI	CCTGCAGG	1 cut(s)	1036
ScaI	AGTACT	1 cut(s)	1828
SchI	GAGTC	3 cut(s)	341, 829, 1849
ScrFI	CCNGG	5 cut(s)	735, 840, 920, 1113, 1230
SdaI	CCTGCAGG	1 cut(s)	1036
SduI	GDGCHC	4 cut(s)	718, 954, 1195, 1414
SfaNI	GCATC	3 cut(s)	874, 1506, 1969
SfcI	CTRYAG	4 cut(s)	472, 1032, 1623, 1764
SfoI	GGCGCC	1 cut(s)	100
SfuI	TTCGAA	1 cut(s)	805
SinI	GGWCC	4 cut(s)	144, 730, 935, 1028
SmiMI	CAYNNNNRTG	5 cut(s)	828, 1656, 1723, 1895, 2091
SmlI	CTYRAG	6 cut(s)	182, 239, 680, 1415, 1814, 2135
SmoI	CTYRAG	6 cut(s)	182, 239, 680, 1415, 1814, 2135
SphI	GCATGC	1 cut(s)	891
Sse8387I	CCTGCAGG	1 cut(s)	1036
SsiI	CCGC	5 cut(s)	892, 1014, 1283, 2038, 2155
SspDI	GGCGCC	1 cut(s)	98
SspI	AATATT	1 cut(s)	993
SspMI	CTAG	7 cut(s)	1188, 1346, 1605, 1641, 1787, 1877, 2069
StyD4I	CCNGG	5 cut(s)	733, 838, 918, 1111, 1228
StyI	CCWWGG	1 cut(s)	1604
TaiI	ACGT	3 cut(s)	42, 792, 2016
TaqI	TCGA	5 cut(s)	36, 83, 805, 1562, 1656
TatI	WGTACW	3 cut(s)	814, 1826, 1951
TauI	GCSGC	1 cut(s)	894
TfiI	GAWTC	5 cut(s)	106, 259, 298, 820, 1157
Tru1I	TTAA	7 cut(s)	521, 860, 1403, 1539, 1809, 1836, 2018
Tru9I	TTAA	7 cut(s)	521, 860, 1403, 1539, 1809, 1836, 2018
TseFI	GTSAC	2 cut(s)	1919, 2044
TseI	GCWGC	8 cut(s)	62, 65, 386, 474, 797, 982, 1001, 1622
Tsp45I	GTSAC	2 cut(s)	1919, 2044
TspDTI	ATGAA	9 cut(s)	119, 668, 734, 740, 812, 1355, 1644, 1725, 1879
TspGWI	ACGGA	2 cut(s)	23, 2045
VneI	GTGCAC	2 cut(s)	714, 1191
VpaK11BI	GGWCC	4 cut(s)	144, 730, 935, 1028
VspI	ATTAAT	1 cut(s)	1403
XapI	RAATTY	3 cut(s)	756, 909, 2029
XceI	RCATGY	2 cut(s)	366, 891
XmaJI	CCTAGG	1 cut(s)	1604
XmnI	GAANNNNTTC	1 cut(s)	1262
XspI	CTAG	7 cut(s)	1188, 1346, 1605, 1641, 1787, 1877, 2069
ZrmI	AGTACT	1 cut(s)	1828
Zsp2I	ATGCAT	2 cut(s)	889, 1696

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.