Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0008324

Physical Location & Seq

Genomic Coordinates Forward (+)

36464294 .. 36473970

Gene Structure 9677 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1286000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2193 bp

ATGGGTACGGAGAGGAAGAGGAAGGTGAGCTTGTTCGACGTGGTGGACGAGACCTCCATCTCATTGGCGAAGATGAGCAAATCGAACGGCGGCGGCGGAGCTGCTGCCAACCAAAACGCTGTGAGCAGTATCATCAATCGGTGGACGGGAAAGCCGTACTCGCAGAGGTACTATGAGATTCTTGAGAAGAGGAAGACGTTGCCGGTTTGGCACCAGAAAGAGGAGTTCTTGCAGGCTCTCAAAGCCAGCCAGTCTCTCATTTTGGTTGGTGAGACTGGTAGTGGTAAAACCACGCAGATTCCCCAATTTGTTTTGGAAGCTGTCGATTCAGAATCTTCAGATAAACGCAAAAAGATGATGGTTGCATGTACCCAACCCCGTAGAGTGGCTGCAATGTCAGTATCCCGTCGTGTTGCTGAAGAGATGGATGTAACCATCGGAGAAGAGGTCGGTTATAGCATCCGTTTTGAAGACTGCAGCAGTTCCAGAACTGTGTTGAAGTATCTAACTGATGGAATGCTTTTAAGAGAAGCAATGACTGATCCACTATTAGAAAGATACAGCGTGATAATTCTTGATGAAGCTCACGAAAGAACTTTAGCAACAGATGTTCTATTTGGGCTGTTGAAGGAAGTGTTGAGAAACAGACCTGACTTGAAGGTGGTGGTGATGAGTGCTACGCTTGAAGCTGAAAAGTTTCAGGGCTATTTCAGCGGTGCACCACTCATGAAAGTTCCTGGAAGGCTTCATCCAGTGGAAATTTTTTACACTGAGGAACCTGAAAGGGATTATCTGGAAGCAGCGATTCGAACGGTTGTGCAGATTCATATGTATGAGACCCCAGGAGATATACTTGTTTTTCTAACAGGAGAGGAGGAGATAGAAGATGCATGCCGCAAAATTAACAAGGAAATTGGAAATCTGGGTGACCAGGTTGGTCCTGTGAAAGTGGTGCCTCTGTATTCAACTCTACCTCCAGCTATGCAGCAGAAAATATTTGATCCTGCTCCTCCTCCTGTAAAAGAAGGTGGAATTCCTGGAAGGAAGATTGTGGTGTCCACAAACATTGCAGAAACTTCTCTGACTATTGATGGAATTGTTTATGTTATTGATCCTGGATTTGCTAAACAAATAGTCTATAACCCAAGAGTGCGTGTTGAATCATTGTTGGTATCTCCAATTTCCAAGGCAAGTGCACATCAGAGGTCAGGTCGTGCTGGAAGAACTCAACCAGGAAAATGTTTCAGACTTTATACTGAGAAAAGTTTCCAGAATGATCTTCAACCACAAACTTATCCTGAAATATTGAGATCCAACCTTGCAAATACAGTTCTTACTTTGAAGAAATTAGGAATAGATGATCTGGTTCATTTTGATTTTATGGATCCCCCTGCTCCCGAGACACTGATGAGAGCACTAGAGGTCTTGAATTATTTGGGAGCATTGGATGATGATGGTAACTTGACCAAGCTGGGGGAGATTATGAGTGAGTTCCCATTGGATCCTCAGATGTCAAAGATGCTTGTAGTCAGCCCTGAGTTCAGCTGCTCAAATGAAATTTTGTCAATATCTGCCATGCTTTCAGTACCCAATTGCTTTGTCCGGCCTAGGGAGGCTCAAAAAGCTGCTGATGAAGCAAAAGCTAGGTTCGGGCACATTGATGGAGATCATCTCACGCTTCTGAACGTGTATCATGCATACAAGCAAAATAATGAGGATCCGTCATGGTGCTATGAAAACTTTGTCAATCAGAGGGCATTGAAGTCCGCAGACAATGTCAGGCAGCAGCTAGTGCGCATTATGGGCCGATTTAACCTCAAGCTGTGCAGTACTGAATTCAATAGTCGCGACTACTACATCAACATAAGAAAGGCCATGCTAGCTGGATATTTCATGCAGGTGGCTCACCTGGAACACACTGGCCACTATCTGACAGTGAAAGATAACCAAGTGGTGCATTTGCATCCGTCGAATTGCTTGGATCACAAGCCAGAATGGGTGATTTACAATGAGTATGTTTTGACAAGCAGGAATTTTATCCGTACAGTGATGGATATTCGTGGGGAGTGGCTAATCGATATAGCACCCCACTACTATGATTTGGCGAATTTTCCTCAGTGTGAGGCCAAGCGTGTTCTTGAGAAGCTATACAGGAAGCGGGAGGACAAGGATGAGAACAAAAACAGAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0010467 GO:0016020 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

AAA_22 DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

731

Amino Acids

82.77

Weight (kDa)

6.51

Isoelectric Point (pI)

46.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	73 - 229	2.3e-10	DEAD/DEAH box helicase
AAA_22	PF13401	83 - 224	1.7e-07	AAA domain
Helicase_C	PF00271	270 - 409	5.4e-09	Helicase conserved C-terminal domain
WHD_HA2	PF04408	472 - 500	2.3e-11	Helicase associated domain (HA2), winged-helix
HA2_C	PF21010	501 - 560	4.8e-14	Helicase associated domain (HA2), ratchet-like
OB_NTP_bind	PF07717	622 - 698	3.9e-24	Oligonucleotide/oligosaccharide-binding (OB)-fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008324)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47250 AT3G62310 AT3G62310 AT3G62310
fragaria_vesca	FvH4_1g26860 FvH4_1g26860
malus_domestica	MD09G1286000.v1.1 MD17G1278600.v1.1
prunus_persica	Prupe.3G007400_v2.0.a1
pyrus_communis	pycom09g19330 pycom17g27620 pycom17g27630
rosa_chinensis	RchiOBHm_Chr3g0487101
rosa_laevigata	RLG00000023033
rosa_multiflora	Rmu_co8377811.1_g000001 Rmu_ssc0000271.1_g000001
rosa_roxburghii	Rroxscaffold_6G00395180
rosa_rugosa	Rorug03G0231000
rosa_samantha	Rh3AG279300 Rh3BG315300 Rh3CG313500 Rh3DG310300
rosa_wichuraiana	Rw3G024760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1888
Acc16I	TGCGCA	1 cut(s)	1796
Acc36I	ACCTGC	1 cut(s)	1888
AccB1I	GGYRCC	2 cut(s)	210, 952
AccII	CGCG	1 cut(s)	1848
AciI	CCGC	7 cut(s)	90, 93, 96, 714, 895, 1767, 2158
AcoI	YGGCCR	1 cut(s)	1921
AcsI	RAATTY	6 cut(s)	759, 1032, 1557, 1835, 2032, 2107
AcuI	CTGAAG	2 cut(s)	321, 438
AfaI	GTAC	7 cut(s)	7, 158, 170, 370, 1587, 1831, 2044
AfiI	CCNNNNNNNGG	4 cut(s)	220, 385, 1473, 1609
AflIII	ACRYGT	1 cut(s)	1686
AjiI	CACGTC	1 cut(s)	40
AjnI	CCWGG	7 cut(s)	736, 841, 930, 1036, 1114, 1231, 1908
Alw21I	GWGCWC	3 cut(s)	721, 1198, 1417
Alw26I	GTCTC	5 cut(s)	44, 258, 266, 830, 1394
Alw44I	GTGCAC	2 cut(s)	717, 1194
Ama87I	CYCGRG	1 cut(s)	1397
AoxI	GGCC	5 cut(s)	1604, 1804, 1872, 1921, 2124
ApaLI	GTGCAC	2 cut(s)	717, 1194
ApoI	RAATTY	6 cut(s)	759, 1032, 1557, 1835, 2032, 2107
Asp700I	GAANNNNTTC	2 cut(s)	696, 1265
AspA2I	CCTAGG	1 cut(s)	1607
AspLEI	GCGC	1 cut(s)	1797
AspS9I	GGNCC	2 cut(s)	938, 1804
AsuHPI	GGTGA	6 cut(s)	37, 281, 679, 938, 1898, 2011
AsuII	TTCGAA	1 cut(s)	808
AsuNHI	GCTAGC	1 cut(s)	1879
AvaI	CYCGRG	1 cut(s)	1397
AvaII	GGWCC	1 cut(s)	938
AvrII	CCTAGG	1 cut(s)	1607
BaeGI	GKGCMC	3 cut(s)	721, 1198, 1656
BalI	TGGCCA	1 cut(s)	1923
BamHI	GGATCC	3 cut(s)	1384, 1501, 1717
BanI	GGYRCC	2 cut(s)	210, 952
BbsI	GAAGAC	2 cut(s)	200, 477
Bbv12I	GWGCWC	3 cut(s)	721, 1198, 1417
BccI	CCATC	9 cut(s)	65, 352, 418, 443, 506, 1085, 1448, 1655, 2044
BceAI	ACGGC	2 cut(s)	103, 139
BciT130I	CCWGG	7 cut(s)	738, 843, 932, 1038, 1116, 1233, 1910
BciVI	GTATCC	1 cut(s)	412
BcoDI	GTCTC	5 cut(s)	44, 258, 266, 830, 1394
BfaI	CTAG	5 cut(s)	1418, 1608, 1644, 1790, 1880
BfmI	CTRYAG	1 cut(s)	475
BfuAI	ACCTGC	1 cut(s)	1888
BfuI	GTATCC	1 cut(s)	412
BglI	GCCNNNNNGGC	1 cut(s)	208
BlnI	CCTAGG	1 cut(s)	1607
BmcAI	AGTACT	1 cut(s)	1831
Bme1390I	CCNGG	7 cut(s)	738, 843, 932, 1038, 1116, 1233, 1910
Bme18I	GGWCC	1 cut(s)	938
BmeT110I	CYCGRG	1 cut(s)	1397
BmgBI	CACGTC	1 cut(s)	40
BmgT120I	GGNCC	2 cut(s)	938, 1804
BmiI	GGNNCC	6 cut(s)	212, 777, 954, 1386, 1503, 1719
BmrFI	CCNGG	7 cut(s)	738, 843, 932, 1038, 1116, 1233, 1910
BmsI	GCATC	4 cut(s)	468, 877, 1509, 1972
BmtI	GCTAGC	1 cut(s)	1883
BpiI	GAAGAC	2 cut(s)	200, 477
BplI	GAGNNNNNCTC	2 cut(s)	1656, 1688
BpmI	CTGGAG	1 cut(s)	960
Bpu14I	TTCGAA	1 cut(s)	808
BpuEI	CTTGAG	3 cut(s)	203, 1802, 2159
Bsa29I	ATCGAT	1 cut(s)	2076
BsaBI	GATNNNNATC	1 cut(s)	1665
BsaI	GGTCTC	2 cut(s)	44, 830
BsaJI	CCNNGG	3 cut(s)	841, 1185, 1607
BsaXI	ACNNNNNCTCC	6 cut(s)	38, 68, 432, 462, 958, 988
Bsc4I	CCNNNNNNNGG	4 cut(s)	220, 385, 1473, 1609
Bse118I	RCCGGY	1 cut(s)	202
Bse1I	ACTGG	4 cut(s)	250, 280, 752, 1924
Bse3DI	GCAATG	3 cut(s)	399, 540, 1065
Bse8I	GATNNNNATC	1 cut(s)	1665
BseBI	CCWGG	7 cut(s)	738, 843, 932, 1038, 1116, 1233, 1910
BseCI	ATCGAT	1 cut(s)	2076
BseDI	CCNNGG	3 cut(s)	841, 1185, 1607
BseGI	GGATG	6 cut(s)	433, 459, 748, 1453, 1963, 2176
BseJI	GATNNNNATC	1 cut(s)	1665
BseLI	CCNNNNNNNGG	4 cut(s)	220, 385, 1473, 1609
BseMI	GCAATG	3 cut(s)	399, 540, 1065
BseMII	CTCAG	5 cut(s)	762, 1248, 1520, 1527, 2129
BseNI	ACTGG	4 cut(s)	250, 280, 752, 1924
BseRI	GAGGAG	5 cut(s)	236, 887, 890, 999, 1002
BseSI	GKGCMC	3 cut(s)	721, 1198, 1656
BseYI	CCCAGC	1 cut(s)	1471
BsgI	GTGCAG	2 cut(s)	839, 1846
Bsh1236I	CGCG	1 cut(s)	1848
BshFI	GGCC	5 cut(s)	1606, 1806, 1874, 1923, 2126
BshNI	GGYRCC	2 cut(s)	210, 952
BshVI	ATCGAT	1 cut(s)	2076
BsiHKAI	GWGCWC	3 cut(s)	721, 1198, 1417
BsiHKCI	CYCGRG	1 cut(s)	1397
BsiSI	CCGG	2 cut(s)	203, 1603
BslI	CCNNNNNNNGG	4 cut(s)	220, 385, 1473, 1609
BsmAI	GTCTC	5 cut(s)	44, 258, 266, 830, 1394
BsmI	GAATGC	1 cut(s)	522
BsnI	GGCC	5 cut(s)	1606, 1806, 1874, 1923, 2126
Bso31I	GGTCTC	2 cut(s)	44, 830
BsoBI	CYCGRG	1 cut(s)	1397
Bsp119I	TTCGAA	1 cut(s)	808
Bsp1286I	GDGCHC	4 cut(s)	721, 1198, 1417, 1656
Bsp68I	TCGCGA	1 cut(s)	1848
BspACI	CCGC	7 cut(s)	90, 93, 96, 714, 895, 1767, 2158
BspANI	GGCC	5 cut(s)	1606, 1806, 1874, 1923, 2126
BspCNI	CTCAG	5 cut(s)	763, 1249, 1519, 1528, 2128
BspDI	ATCGAT	1 cut(s)	2076
BspFNI	CGCG	1 cut(s)	1848
BspHI	TCATGA	1 cut(s)	726
BspLI	GGNNCC	6 cut(s)	212, 777, 954, 1386, 1503, 1719
BspMAI	CTGCAG	1 cut(s)	479
BspMI	ACCTGC	1 cut(s)	1888
BspOI	GCTAGC	1 cut(s)	1883
BspT104I	TTCGAA	1 cut(s)	808
BspT107I	GGYRCC	2 cut(s)	210, 952
BspTNI	GGTCTC	2 cut(s)	44, 830
BsrDI	GCAATG	3 cut(s)	399, 540, 1065
BsrFI	RCCGGY	1 cut(s)	202
BsrI	ACTGG	4 cut(s)	250, 280, 752, 1924
BssAI	RCCGGY	1 cut(s)	202
BssECI	CCNNGG	3 cut(s)	841, 1185, 1607
BssT1I	CCWWGG	2 cut(s)	1185, 1607
Bst2UI	CCWGG	7 cut(s)	738, 843, 932, 1038, 1116, 1233, 1910
Bst4CI	ACNGT	5 cut(s)	493, 814, 1330, 1936, 2047
Bst6I	CTCTTC	4 cut(s)	11, 182, 414, 438
BstAPI	GCANNNNNTGC	1 cut(s)	1792
BstBI	TTCGAA	1 cut(s)	808
BstC8I	GCNNGC	4 cut(s)	234, 247, 892, 1881
BstDEI	CTNAG	5 cut(s)	771, 1257, 1506, 1536, 2115
BstEII	GGTNACC	1 cut(s)	926
BstF5I	GGATG	6 cut(s)	433, 459, 748, 1453, 1963, 2176
BstFNI	CGCG	1 cut(s)	1848
BstHHI	GCGC	1 cut(s)	1797
BstMAI	GTCTC	5 cut(s)	44, 258, 266, 830, 1394
BstMWI	GCNNNNNNNGC	9 cut(s)	160, 208, 242, 711, 1622, 1634, 1792, 1803, 1880
BstNI	CCWGG	7 cut(s)	738, 843, 932, 1038, 1116, 1233, 1910
BstNSI	RCATGY	2 cut(s)	369, 894
BstPI	GGTNACC	1 cut(s)	926
BstSCI	CCNGG	7 cut(s)	736, 841, 930, 1036, 1114, 1231, 1908
BstSFI	CTRYAG	1 cut(s)	475
BstSLI	GKGCMC	3 cut(s)	721, 1198, 1656
BstUI	CGCG	1 cut(s)	1848
BstV2I	GAAGAC	2 cut(s)	200, 477
BstX2I	RGATCY	4 cut(s)	1310, 1384, 1501, 1717
BstXI	CCANNNNNNTGG	1 cut(s)	64
BstYI	RGATCY	4 cut(s)	1310, 1384, 1501, 1717
Bsu15I	ATCGAT	1 cut(s)	2076
BsuI	GTATCC	1 cut(s)	412
BsuRI	GGCC	5 cut(s)	1606, 1806, 1874, 1923, 2126
BsuTUI	ATCGAT	1 cut(s)	2076
BtrI	CACGTC	1 cut(s)	40
BtsCI	GGATG	6 cut(s)	433, 459, 748, 1453, 1963, 2176
BtsIMutI	CAGTG	7 cut(s)	759, 768, 1403, 1917, 1941, 2052, 2123
BtuMI	TCGCGA	1 cut(s)	1848
BveI	ACCTGC	1 cut(s)	1888
Cac8I	GCNNGC	4 cut(s)	234, 247, 892, 1881
CciI	TCATGA	1 cut(s)	726
CfoI	GCGC	1 cut(s)	1797
Cfr10I	RCCGGY	1 cut(s)	202
Cfr13I	GGNCC	2 cut(s)	938, 1804
ClaI	ATCGAT	1 cut(s)	2076
CsiI	ACCWGGT	1 cut(s)	930
Csp6I	GTAC	7 cut(s)	6, 157, 169, 369, 1586, 1830, 2043
CspCI	CAANNNNNGTGG	2 cut(s)	1048, 1083
CviAII	CATG	8 cut(s)	366, 727, 891, 1576, 1694, 1725, 1876, 1894
CviQI	GTAC	7 cut(s)	6, 157, 169, 369, 1586, 1830, 2043
DdeI	CTNAG	5 cut(s)	771, 1257, 1506, 1536, 2115
EaeI	YGGCCR	1 cut(s)	1921
Eam1104I	CTCTTC	4 cut(s)	11, 182, 414, 438
EarI	CTCTTC	4 cut(s)	11, 182, 414, 438
EciI	GGCGGA	1 cut(s)	111
Eco130I	CCWWGG	2 cut(s)	1185, 1607
Eco31I	GGTCTC	2 cut(s)	44, 830
Eco47I	GGWCC	1 cut(s)	938
Eco57I	CTGAAG	2 cut(s)	321, 438
Eco88I	CYCGRG	1 cut(s)	1397
Eco91I	GGTNACC	1 cut(s)	926
EcoO65I	GGTNACC	1 cut(s)	926
EcoRI	GAATTC	2 cut(s)	1032, 1835
EcoRII	CCWGG	7 cut(s)	736, 841, 930, 1036, 1114, 1231, 1908
EcoT14I	CCWWGG	2 cut(s)	1185, 1607
EcoT22I	ATGCAT	2 cut(s)	892, 1699
ErhI	CCWWGG	2 cut(s)	1185, 1607
FaeI	CATG	8 cut(s)	369, 730, 894, 1579, 1697, 1728, 1879, 1897
FalI	AAGNNNNNCTT	2 cut(s)	14, 46
FatI	CATG	8 cut(s)	365, 726, 890, 1575, 1693, 1724, 1875, 1893
FauI	CCCGC	1 cut(s)	2151
FauNDI	CATATG	1 cut(s)	828
FokI	GGATG	6 cut(s)	440, 446, 735, 1460, 1950, 2183
FspAI	RTGCGCAY	1 cut(s)	1796
FspBI	CTAG	5 cut(s)	1418, 1608, 1644, 1790, 1880
FspI	TGCGCA	1 cut(s)	1796
GlaI	GCGC	1 cut(s)	1796
GsaI	CCCAGC	1 cut(s)	1475
GsuI	CTGGAG	1 cut(s)	960
HaeIII	GGCC	5 cut(s)	1606, 1806, 1874, 1923, 2126
HapII	CCGG	2 cut(s)	203, 1603
HhaI	GCGC	1 cut(s)	1797
Hin1II	CATG	8 cut(s)	369, 730, 894, 1579, 1697, 1728, 1879, 1897
Hin6I	GCGC	1 cut(s)	1795
HinP1I	GCGC	1 cut(s)	1795
HinfI	GANTC	7 cut(s)	178, 298, 326, 332, 805, 823, 1160
HpaII	CCGG	2 cut(s)	203, 1603
HphI	GGTGA	6 cut(s)	37, 281, 679, 938, 1898, 2011
Hpy166II	GTNNAC	5 cut(s)	46, 144, 719, 1059, 1196
Hpy8I	GTNNAC	5 cut(s)	46, 144, 719, 1059, 1196
Hpy99I	CGWCG	3 cut(s)	41, 411, 1972
HpyAV	CCTTC	6 cut(s)	16, 622, 652, 735, 1019, 1035
HpyCH4III	ACNGT	5 cut(s)	493, 814, 1330, 1936, 2047
HpyCH4IV	ACGT	3 cut(s)	39, 197, 1686
HpyF10VI	GCNNNNNNNGC	9 cut(s)	160, 208, 242, 711, 1622, 1634, 1792, 1803, 1880
HpyF3I	CTNAG	5 cut(s)	771, 1257, 1506, 1536, 2115
HpySE526I	ACGT	3 cut(s)	39, 197, 1686
Hsp92II	CATG	8 cut(s)	369, 730, 894, 1579, 1697, 1728, 1879, 1897
HspAI	GCGC	1 cut(s)	1795
LmnI	GCTCC	4 cut(s)	98, 1012, 1399, 1439
LweI	GCATC	4 cut(s)	468, 877, 1509, 1972
MabI	ACCWGGT	1 cut(s)	930
MaeI	CTAG	5 cut(s)	1418, 1608, 1644, 1790, 1880
MaeII	ACGT	3 cut(s)	39, 197, 1686
MaeIII	GTNAC	3 cut(s)	430, 926, 1457
MfeI	CAATTG	1 cut(s)	1591
MflI	RGATCY	4 cut(s)	1310, 1384, 1501, 1717
MhlI	GDGCHC	4 cut(s)	721, 1198, 1417, 1656
MlsI	TGGCCA	1 cut(s)	1923
MluNI	TGGCCA	1 cut(s)	1923
MmeI	TCCRAC	1 cut(s)	1338
Mox20I	TGGCCA	1 cut(s)	1923
Mph1103I	ATGCAT	2 cut(s)	892, 1699
MroXI	GAANNNNTTC	2 cut(s)	696, 1265
MscI	TGGCCA	1 cut(s)	1923
MseI	TTAA	3 cut(s)	524, 903, 1812
MslI	CAYNNNNRTG	4 cut(s)	831, 1659, 1898, 2094
Msp20I	TGGCCA	1 cut(s)	1923
MspA1I	CMGCKG	2 cut(s)	714, 1545
MspI	CCGG	2 cut(s)	203, 1603
MspR9I	CCNGG	7 cut(s)	738, 843, 932, 1038, 1116, 1233, 1910
MunI	CAATTG	1 cut(s)	1591
Mva1269I	GAATGC	1 cut(s)	522
MvaI	CCWGG	7 cut(s)	738, 843, 932, 1038, 1116, 1233, 1910
MvnI	CGCG	1 cut(s)	1848
MwoI	GCNNNNNNNGC	9 cut(s)	160, 208, 242, 711, 1622, 1634, 1792, 1803, 1880
NdeI	CATATG	1 cut(s)	828
NheI	GCTAGC	1 cut(s)	1879
NlaIII	CATG	8 cut(s)	369, 730, 894, 1579, 1697, 1728, 1879, 1897
NlaIV	GGNNCC	6 cut(s)	212, 777, 954, 1386, 1503, 1719
NmuCI	GTSAC	1 cut(s)	926
NruI	TCGCGA	1 cut(s)	1848
NsbI	TGCGCA	1 cut(s)	1796
NsiI	ATGCAT	2 cut(s)	892, 1699
NspI	RCATGY	2 cut(s)	369, 894
NspV	TTCGAA	1 cut(s)	808
PaeI	GCATGC	1 cut(s)	894
PagI	TCATGA	1 cut(s)	726
PaqCI	CACCTGC	1 cut(s)	1888
PcsI	WCGNNNNNNNCGW	2 cut(s)	45, 152
PctI	GAATGC	1 cut(s)	522
PdmI	GAANNNNTTC	2 cut(s)	696, 1265
PfeI	GAWTC	7 cut(s)	178, 298, 326, 332, 805, 823, 1160
PflFI	GACNNNGTC	1 cut(s)	1775
PfoI	TCCNGGA	3 cut(s)	736, 1036, 1114
Psp6I	CCWGG	7 cut(s)	736, 841, 930, 1036, 1114, 1231, 1908
PspEI	GGTNACC	1 cut(s)	926
PspFI	CCCAGC	1 cut(s)	1471
PspGI	CCWGG	7 cut(s)	736, 841, 930, 1036, 1114, 1231, 1908
PspN4I	GGNNCC	6 cut(s)	212, 777, 954, 1386, 1503, 1719
PspPI	GGNCC	2 cut(s)	938, 1804
PstI	CTGCAG	1 cut(s)	479
PsuI	RGATCY	4 cut(s)	1310, 1384, 1501, 1717
PsyI	GACNNNGTC	1 cut(s)	1775
PvuII	CAGCTG	1 cut(s)	1545
RruI	TCGCGA	1 cut(s)	1848
RsaI	GTAC	7 cut(s)	7, 158, 170, 370, 1587, 1831, 2044
RsaNI	GTAC	7 cut(s)	6, 157, 169, 369, 1586, 1830, 2043
RseI	CAYNNNNRTG	4 cut(s)	831, 1659, 1898, 2094
SaqAI	TTAA	3 cut(s)	524, 903, 1812
Sau96I	GGNCC	2 cut(s)	938, 1804
ScaI	AGTACT	1 cut(s)	1831
ScrFI	CCNGG	7 cut(s)	738, 843, 932, 1038, 1116, 1233, 1910
SduI	GDGCHC	4 cut(s)	721, 1198, 1417, 1656
SexAI	ACCWGGT	1 cut(s)	930
SfaNI	GCATC	4 cut(s)	468, 877, 1509, 1972
SfcI	CTRYAG	1 cut(s)	475
SfuI	TTCGAA	1 cut(s)	808
SinI	GGWCC	1 cut(s)	938
SmiMI	CAYNNNNRTG	4 cut(s)	831, 1659, 1898, 2094
SmlI	CTYRAG	3 cut(s)	182, 1817, 2138
SmoI	CTYRAG	3 cut(s)	182, 1817, 2138
SphI	GCATGC	1 cut(s)	894
SsiI	CCGC	7 cut(s)	90, 93, 96, 714, 895, 1767, 2158
SspI	AATATT	2 cut(s)	996, 1305
SspMI	CTAG	5 cut(s)	1418, 1608, 1644, 1790, 1880
StyD4I	CCNGG	7 cut(s)	736, 841, 930, 1036, 1114, 1231, 1908
StyI	CCWWGG	2 cut(s)	1185, 1607
TaaI	ACNGT	5 cut(s)	493, 814, 1330, 1936, 2047
TaiI	ACGT	3 cut(s)	42, 200, 1689
TaqI	TCGA	6 cut(s)	36, 83, 324, 808, 1970, 2076
TatI	WGTACW	1 cut(s)	1829
TauI	GCSGC	3 cut(s)	93, 96, 897
TfiI	GAWTC	7 cut(s)	178, 298, 326, 332, 805, 823, 1160
Tru1I	TTAA	3 cut(s)	524, 903, 1812
Tru9I	TTAA	3 cut(s)	524, 903, 1812
TscAI	CASTG	7 cut(s)	759, 775, 1410, 1924, 1941, 2052, 2123
TseFI	GTSAC	1 cut(s)	926
Tsp45I	GTSAC	1 cut(s)	926
TspDTI	ATGAA	9 cut(s)	594, 737, 743, 815, 1358, 1569, 1647, 1749, 1882
TspGWI	ACGGA	5 cut(s)	23, 452, 1710, 1956, 2030
TspRI	CASTG	7 cut(s)	759, 775, 1410, 1924, 1941, 2052, 2123
Tth111I	GACNNNGTC	1 cut(s)	1775
VneI	GTGCAC	2 cut(s)	717, 1194
VpaK11BI	GGWCC	1 cut(s)	938
XapI	RAATTY	6 cut(s)	759, 1032, 1557, 1835, 2032, 2107
XceI	RCATGY	2 cut(s)	369, 894
XmaJI	CCTAGG	1 cut(s)	1607
XmnI	GAANNNNTTC	2 cut(s)	696, 1265
XspI	CTAG	5 cut(s)	1418, 1608, 1644, 1790, 1880
ZrmI	AGTACT	1 cut(s)	1831
Zsp2I	ATGCAT	2 cut(s)	892, 1699

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.