Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0008324

Physical Location & Seq

Genomic Coordinates Forward (+)

16186057 .. 16190358

Gene Structure 4302 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00395180.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2322 bp

ATGGGTACGGAGAGGAAGAGGAAGGTGAGCTTGTTCGACGTGGTGGACGAGACCACTGTTGCAGCTGCGAAGATGAACAAATCGAACGGTGGTGGAGGCGCCGTGAATCACAACAACCCGATGAGCAGTCTGATCAATAGGTGGACCGGAAAGCCCTACTCGCAAAGGTACTATGAGATCCTTGAGAAGAGGAAGACTTTGCCTGTTTGGCACCAGAAGGAGGAGTTCTTTCAGGTTCTCAAGAAAAGTCAGTCGCTGATTCTGGTTGGTGAGACTGGTAGTGGTAAAACCACTCAGATTCCTCAATTTGTTTTGGAAGCTGTTGATAGGGAGTCTTCAGATAAAAGCAGGAAGATGATTGCATGTACCCAGCCTCGTAGGGTGGCTGCAATGTCAGTGTCTCGCCGTGTTGCTGAAGAGATGGATGTTACCATTGGGGAAGAGGTTGGTTACAGCATTCGTTTTGAAGACTGCAGCAGTGCCAGAACAGTATTGAAGTATCTTACAGATGGTATGCTTTTAAGAGAAGCCATGACAGATCCACTGTTAGAACGATACAGTGTAATAATTCTTGATGAGGCTCATGAGAGGACTTTGGCAACAGATGTTCTGTTTGGGCTTCTGAAGGAAGTATTGAAAAATAGACCTGATATGAAGCTGGTTGTTATGAGTGCTACACTTGAGGCTGAGAAATTTCAGGGTTATTTCAACGGTGCACCACTAATGAAGGTCCCTGGCAGGCTTCATCCAGTTGAAATTTTTTACACTGAGGAACCTGAGAGGGACTACCTGGAGGCAGCAATTCGAACTGTTGTACAGATTCATATGTATGAGACTCCAGGAGATATACTTGTTTTTTTAACTGGAGAAGAGGAGATAGAAGATGCATGCCGCAAAATCAACAAAGAAATTTCAAACCTGGGTGATCAGGTTGGTCCAGTGAAAGTGGTGCCCCTGTATTCAACTCTCCCTCCAGCTATGCAGCAGAAAATATTTGACGCTGCTCCTCCTCCCGCAACAGAAGGTGGTCCTGCAGGAAGGAAAATTGTGGTGTCAACTAACATCGCAGAAACTTCTCTGACCATAGATGGGATCGTGTATGTTATTGATCCAGGTTTTTCTAAACAGAAAGTTTATAACCCACGAGTGCGTGTTGAATCCTTATTGGTATCTCCGATTTCTAAGGCTAGTGCACATCAGAGGTCAGGGCGTGCTGGAAGAACTCAGCCAGGCAAATGTTTCAGACTTTATACGGAGAAAAGTTTCCATAATGATCTTGTACCGCAGACATATCCAGAAATACTGAGATCAAATCTTGCAAATACAGTGCTTACTTTGAAGAAACTAGGAATAGATGATTTGGTTCATTTTGATTTTATGGATCCCCCTGCTCCAGAGACATTAATGAGAGCACTCGAGGTTTTGAATTATTTGGGTGCATTGGATGATGATGGTAACTTGACCAAGCTGGGTGAGATTATGAGCGAGTTCCCTCTGGATCCTCAGATGTCAAAGATGCTTGTTGTTAGCCCCGAGTTTAACTGCTCAAATGAGATTTTGTCGATTTCTGCCATGCTTTCAGTAGATGAGGTCAAGCTGCACGATGCATGCTTGTTGATATGCGCAGACAAATCATTTGTCTGCGCTCATGATGTCTTAACTACAACATCTGTTGTCATCAGCAACTGTCTTGTCTGGGTTCCATGCCCCCCGGTTGGCATGCACCTATGGATTTCTAAGGAGGCTCAGAAAGCTGCAGATGAAGCAAAAGCTAGGTTTGGACACATCGATGGAGATCATCTCACACTGCTGAATGTGTATCATGCATACAAGCAAAACAATGAAGATCCATCGTGGTGCTATGAGAACTTTGTCAATCAGAGGGCATTGAAGTCTGCAGACAATGTTAGACAACAGCTAGTGCGCATTATGGCTCGGTTTAATCTCAAGCTATGCAGTACTGATTTTAAGAGTCGGGACTACTACATCAACATCAGAAAGGCTATGCTAGCTGGGTACTTCATGCAGGTGGCTCATCTGGAACGCACTGGTCACTACCTTACTGTGAAGGATAACCAAGTTGTACATTTGCATCCATCAAATTGCTTGGATCACAAGCCAGAGTGGGTGATTTACAATGAGTACGTCTTAACGAGCAGGAATTTTATCCGCACAGTGACAGATATCCGTGGTGAATGGCTAGTTGATATAGCACCACACTACTATGATTTGGCCAACTTTCCCCAGTGTGAGGCCAAGCGAGTTCTTGAGAAGCTTTACAGGAAGCGGGAAGACAAGGAGGATAACAGAAACAGAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0010467 GO:0016020 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

AAA_22 DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

773

Amino Acids

87.58

Weight (kDa)

6.5

Isoelectric Point (pI)

42.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	73 - 228	6.7e-10	DEAD/DEAH box helicase
AAA_22	PF13401	83 - 213	1.7e-07	AAA domain
Helicase_C	PF00271	269 - 408	1.4e-09	Helicase conserved C-terminal domain
WHD_HA2	PF04408	471 - 499	2.5e-11	Helicase associated domain (HA2), winged-helix
HA2_C	PF21010	500 - 603	2.6e-08	Helicase associated domain (HA2), ratchet-like
OB_NTP_bind	PF07717	665 - 741	9.4e-25	Oligonucleotide/oligosaccharide-binding (OB)-fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008324)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47250 AT3G62310 AT3G62310 AT3G62310
fragaria_vesca	FvH4_1g26860 FvH4_1g26860
malus_domestica	MD09G1286000.v1.1 MD17G1278600.v1.1
prunus_persica	Prupe.3G007400_v2.0.a1
pyrus_communis	pycom09g19330 pycom17g27620 pycom17g27630
rosa_chinensis	RchiOBHm_Chr3g0487101
rosa_laevigata	RLG00000023033
rosa_multiflora	Rmu_co8377811.1_g000001 Rmu_ssc0000271.1_g000001
rosa_roxburghii	Rroxscaffold_6G00395180
rosa_rugosa	Rorug03G0231000
rosa_samantha	Rh3AG279300 Rh3BG315300 Rh3CG313500 Rh3DG310300
rosa_wichuraiana	Rw3G024760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1137
AarI	CACCTGC	1 cut(s)	2017
Acc16I	TGCGCA	2 cut(s)	1624, 1925
Acc36I	ACCTGC	1 cut(s)	2017
AccB1I	GGYRCC	3 cut(s)	98, 210, 949
AciI	CCGC	5 cut(s)	892, 1014, 1283, 2170, 2287
AcoI	YGGCCR	1 cut(s)	2232
AcsI	RAATTY	4 cut(s)	692, 756, 909, 2161
AcuI	CTGAAG	3 cut(s)	321, 435, 644
AcyI	GRCGYC	1 cut(s)	99
AfaI	GTAC	9 cut(s)	7, 170, 367, 816, 1281, 1960, 2018, 2085, 2144
AfiI	CCNNNNNNNGG	4 cut(s)	220, 1089, 1715, 2251
AjiI	CACGTC	1 cut(s)	40
AjnI	CCWGG	6 cut(s)	733, 789, 838, 918, 1111, 1228
Alw21I	GWGCWC	3 cut(s)	718, 1195, 1414
Alw26I	GTCTC	5 cut(s)	44, 266, 405, 827, 1391
Alw44I	GTGCAC	2 cut(s)	714, 1191
AlwNI	CAGNNNCTG	2 cut(s)	256, 1686
Ama87I	CYCGRG	2 cut(s)	1415, 1532
AoxI	GGCC	2 cut(s)	2232, 2253
ApaLI	GTGCAC	2 cut(s)	714, 1191
ApeKI	GCWGC	9 cut(s)	62, 65, 386, 474, 797, 982, 1001, 1597, 1754
ApoI	RAATTY	4 cut(s)	692, 756, 909, 2161
ArsI	GACNNNNNNTTYG	4 cut(s)	1619, 1623, 1651, 1655
AseI	ATTAAT	1 cut(s)	1403
Asp700I	GAANNNNTTC	1 cut(s)	1262
AspLEI	GCGC	4 cut(s)	101, 1625, 1646, 1926
AspS9I	GGNCC	4 cut(s)	144, 730, 935, 1028
AsuC2I	CCSGG	1 cut(s)	1712
AsuHPI	GGTGA	6 cut(s)	37, 281, 935, 1484, 2140, 2204
AsuII	TTCGAA	1 cut(s)	805
AsuNHI	GCTAGC	1 cut(s)	2008
AvaI	CYCGRG	2 cut(s)	1415, 1532
AvaII	GGWCC	4 cut(s)	144, 730, 935, 1028
BaeGI	GKGCMC	3 cut(s)	718, 954, 1195
BalI	TGGCCA	1 cut(s)	2234
BamHI	GGATCC	2 cut(s)	1381, 1498
BanI	GGYRCC	3 cut(s)	98, 210, 949
BauI	CACGAG	1 cut(s)	1143
BbsI	GAAGAC	4 cut(s)	200, 327, 474, 2298
Bbv12I	GWGCWC	3 cut(s)	718, 1195, 1414
BbvI	GCAGC	9 cut(s)	52, 74, 373, 486, 809, 988, 994, 1584, 1741
BccI	CCATC	7 cut(s)	415, 503, 1082, 1445, 1784, 1858, 2104
BceAI	ACGGC	2 cut(s)	86, 390
BciT130I	CCWGG	6 cut(s)	735, 791, 840, 920, 1113, 1230
BclI	TGATCA	2 cut(s)	132, 925
BcnI	CCSGG	1 cut(s)	1712
BcoDI	GTCTC	5 cut(s)	44, 266, 405, 827, 1391
BfaI	CTAG	6 cut(s)	1188, 1346, 1773, 1919, 2009, 2201
BfmI	CTRYAG	4 cut(s)	472, 1032, 1755, 1896
BfoI	RGCGCY	1 cut(s)	102
BfuAI	ACCTGC	1 cut(s)	2017
BglI	GCCNNNNNGGC	1 cut(s)	208
BmcAI	AGTACT	1 cut(s)	1960
Bme1390I	CCNGG	7 cut(s)	735, 791, 840, 920, 1113, 1230, 1712
Bme18I	GGWCC	4 cut(s)	144, 730, 935, 1028
BmeT110I	CYCGRG	2 cut(s)	1415, 1532
BmgBI	CACGTC	1 cut(s)	40
BmgT120I	GGNCC	4 cut(s)	144, 730, 935, 1028
BmiI	GGNNCC	8 cut(s)	100, 212, 732, 774, 951, 1383, 1500, 1701
BmrFI	CCNGG	7 cut(s)	735, 791, 840, 920, 1113, 1230, 1712
BmrI	ACTGGG	1 cut(s)	2239
BmsI	GCATC	4 cut(s)	874, 1506, 1594, 2101
BmtI	GCTAGC	1 cut(s)	2012
BmuI	ACTGGG	1 cut(s)	2239
BpiI	GAAGAC	4 cut(s)	200, 327, 474, 2298
BplI	GAGNNNNNCTC	2 cut(s)	1785, 1817
BpmI	CTGGAG	5 cut(s)	812, 822, 885, 957, 1377
Bpu14I	TTCGAA	1 cut(s)	805
BpuEI	CTTGAG	5 cut(s)	203, 224, 701, 1931, 2288
BpuMI	CCSGG	1 cut(s)	1712
Bsa29I	ATCGAT	1 cut(s)	1788
BsaBI	GATNNNNATC	1 cut(s)	1794
BsaHI	GRCGYC	1 cut(s)	99
BsaI	GGTCTC	1 cut(s)	44
BsaJI	CCNNGG	4 cut(s)	733, 919, 1710, 2188
BsaWI	WCCGGW	1 cut(s)	146
BsaXI	ACNNNNNCTCC	4 cut(s)	87, 117, 955, 985
Bsc4I	CCNNNNNNNGG	4 cut(s)	220, 1089, 1715, 2251
Bse1I	ACTGG	6 cut(s)	280, 749, 868, 938, 2053, 2245
Bse3DI	GCAATG	1 cut(s)	396
Bse8I	GATNNNNATC	1 cut(s)	1794
BseBI	CCWGG	6 cut(s)	735, 791, 840, 920, 1113, 1230
BseCI	ATCGAT	1 cut(s)	1788
BseDI	CCNNGG	4 cut(s)	733, 919, 1710, 2188
BseGI	GGATG	4 cut(s)	430, 745, 1450, 2092
BseJI	GATNNNNATC	1 cut(s)	1794
BseLI	CCNNNNNNNGG	4 cut(s)	220, 1089, 1715, 2251
BseMI	GCAATG	1 cut(s)	396
BseMII	CTCAG	8 cut(s)	308, 678, 759, 768, 1238, 1295, 1517, 1760
BseNI	ACTGG	6 cut(s)	280, 749, 868, 938, 2053, 2245
BseRI	GAGGAG	4 cut(s)	236, 887, 996, 999
BseSI	GKGCMC	3 cut(s)	718, 954, 1195
BseXI	GCAGC	9 cut(s)	52, 74, 373, 486, 809, 988, 994, 1584, 1741
BseYI	CCCAGC	3 cut(s)	369, 1468, 2012
BsgI	GTGCAG	1 cut(s)	1583
BshFI	GGCC	2 cut(s)	2234, 2255
BshNI	GGYRCC	3 cut(s)	98, 210, 949
BshVI	ATCGAT	1 cut(s)	1788
BsiHKAI	GWGCWC	3 cut(s)	718, 1195, 1414
BsiHKCI	CYCGRG	2 cut(s)	1415, 1532
BsiSI	CCGG	2 cut(s)	147, 1712
BslFI	GGGAC	3 cut(s)	716, 797, 1991
BslI	CCNNNNNNNGG	4 cut(s)	220, 1089, 1715, 2251
BsmAI	GTCTC	5 cut(s)	44, 266, 405, 827, 1391
BsmFI	GGGAC	3 cut(s)	716, 797, 1991
BsmI	GAATGC	1 cut(s)	456
BsnI	GGCC	2 cut(s)	2234, 2255
Bso31I	GGTCTC	1 cut(s)	44
BsoBI	CYCGRG	2 cut(s)	1415, 1532
Bsp119I	TTCGAA	1 cut(s)	805
Bsp1286I	GDGCHC	4 cut(s)	718, 954, 1195, 1414
Bsp1407I	TGTACA	2 cut(s)	814, 2083
BspACI	CCGC	5 cut(s)	892, 1014, 1283, 2170, 2287
BspANI	GGCC	2 cut(s)	2234, 2255
BspCNI	CTCAG	8 cut(s)	307, 679, 760, 769, 1237, 1296, 1516, 1759
BspDI	ATCGAT	1 cut(s)	1788
BspHI	TCATGA	2 cut(s)	583, 1648
BspLI	GGNNCC	8 cut(s)	100, 212, 732, 774, 951, 1383, 1500, 1701
BspMAI	CTGCAG	4 cut(s)	476, 1036, 1759, 1900
BspMI	ACCTGC	1 cut(s)	2017
BspOI	GCTAGC	1 cut(s)	2012
BspT104I	TTCGAA	1 cut(s)	805
BspT107I	GGYRCC	3 cut(s)	98, 210, 949
BspTNI	GGTCTC	1 cut(s)	44
BsrDI	GCAATG	1 cut(s)	396
BsrGI	TGTACA	2 cut(s)	814, 2083
BsrI	ACTGG	6 cut(s)	280, 749, 868, 938, 2053, 2245
BssECI	CCNNGG	4 cut(s)	733, 919, 1710, 2188
BssNI	GRCGYC	1 cut(s)	99
BssSI	CACGAG	1 cut(s)	1143
Bst2BI	CACGAG	1 cut(s)	1143
Bst2UI	CCWGG	6 cut(s)	735, 791, 840, 920, 1113, 1230
Bst6I	CTCTTC	5 cut(s)	11, 182, 411, 435, 864
BstACI	GRCGYC	1 cut(s)	99
BstAUI	TGTACA	2 cut(s)	814, 2083
BstBI	TTCGAA	1 cut(s)	805
BstC8I	GCNNGC	6 cut(s)	740, 889, 1212, 1609, 1721, 2010
BstDSI	CCRYGG	1 cut(s)	2188
BstF5I	GGATG	4 cut(s)	430, 745, 1450, 2092
BstH2I	RGCGCY	1 cut(s)	102
BstHHI	GCGC	4 cut(s)	101, 1625, 1646, 1926
BstMAI	GTCTC	5 cut(s)	44, 266, 405, 827, 1391
BstMWI	GCNNNNNNNGC	5 cut(s)	160, 208, 1751, 1763, 2009
BstNI	CCWGG	6 cut(s)	735, 791, 840, 920, 1113, 1230
BstNSI	RCATGY	4 cut(s)	366, 891, 1611, 1723
BstSCI	CCNGG	7 cut(s)	733, 789, 838, 918, 1111, 1228, 1710
BstSFI	CTRYAG	4 cut(s)	472, 1032, 1755, 1896
BstSLI	GKGCMC	3 cut(s)	718, 954, 1195
BstV1I	GCAGC	9 cut(s)	52, 74, 373, 486, 809, 988, 994, 1584, 1741
BstV2I	GAAGAC	4 cut(s)	200, 327, 474, 2298
BstX2I	RGATCY	5 cut(s)	177, 538, 1381, 1498, 1846
BstYI	RGATCY	5 cut(s)	177, 538, 1381, 1498, 1846
Bsu15I	ATCGAT	1 cut(s)	1788
BsuRI	GGCC	2 cut(s)	2234, 2255
BsuTUI	ATCGAT	1 cut(s)	1788
BtgI	CCRYGG	1 cut(s)	2188
BtgZI	GCGATG	1 cut(s)	1048
BtrI	CACGTC	1 cut(s)	40
BtsCI	GGATG	4 cut(s)	430, 745, 1450, 2092
BtsI	GCAGTG	2 cut(s)	484, 1805
BveI	ACCTGC	1 cut(s)	2017
Cac8I	GCNNGC	6 cut(s)	740, 889, 1212, 1609, 1721, 2010
CaiI	CAGNNNCTG	2 cut(s)	256, 1686
CciI	TCATGA	2 cut(s)	583, 1648
CfoI	GCGC	4 cut(s)	101, 1625, 1646, 1926
Cfr13I	GGNCC	4 cut(s)	144, 730, 935, 1028
ClaI	ATCGAT	1 cut(s)	1788
CseI	GACGC	1 cut(s)	1007
Csp6I	GTAC	9 cut(s)	6, 169, 366, 815, 1280, 1959, 2017, 2084, 2143
CviQI	GTAC	9 cut(s)	6, 169, 366, 815, 1280, 1959, 2017, 2084, 2143
DinI	GGCGCC	1 cut(s)	100
EaeI	YGGCCR	1 cut(s)	2232
Eam1104I	CTCTTC	5 cut(s)	11, 182, 411, 435, 864
EarI	CTCTTC	5 cut(s)	11, 182, 411, 435, 864
Eco31I	GGTCTC	1 cut(s)	44
Eco32I	GATATC	1 cut(s)	2185
Eco47I	GGWCC	4 cut(s)	144, 730, 935, 1028
Eco57I	CTGAAG	3 cut(s)	321, 435, 644
Eco88I	CYCGRG	2 cut(s)	1415, 1532
EcoO109I	RGGNCCY	1 cut(s)	730
EcoRII	CCWGG	6 cut(s)	733, 789, 838, 918, 1111, 1228
EcoRV	GATATC	1 cut(s)	2185
EcoT22I	ATGCAT	3 cut(s)	889, 1609, 1828
EgeI	GGCGCC	1 cut(s)	100
EheI	GGCGCC	1 cut(s)	100
FalI	AAGNNNNNCTT	2 cut(s)	14, 46
FaqI	GGGAC	3 cut(s)	716, 797, 1991
FauI	CCCGC	2 cut(s)	1021, 2280
FauNDI	CATATG	1 cut(s)	825
FbaI	TGATCA	2 cut(s)	132, 925
FokI	GGATG	4 cut(s)	437, 732, 1457, 2079
FspAI	RTGCGCAY	1 cut(s)	1925
FspBI	CTAG	6 cut(s)	1188, 1346, 1773, 1919, 2009, 2201
FspI	TGCGCA	2 cut(s)	1624, 1925
GlaI	GCGC	4 cut(s)	100, 1624, 1645, 1925
GsaI	CCCAGC	3 cut(s)	373, 1472, 2016
GsuI	CTGGAG	5 cut(s)	812, 822, 885, 957, 1377
HaeII	RGCGCY	1 cut(s)	102
HaeIII	GGCC	2 cut(s)	2234, 2255
HapII	CCGG	2 cut(s)	147, 1712
HgaI	GACGC	1 cut(s)	1007
HhaI	GCGC	4 cut(s)	101, 1625, 1646, 1926
Hin1I	GRCGYC	1 cut(s)	99
Hin6I	GCGC	4 cut(s)	99, 1623, 1644, 1924
HinP1I	GCGC	4 cut(s)	99, 1623, 1644, 1924
HincII	GTYRAC	1 cut(s)	1056
HindII	GTYRAC	1 cut(s)	1056
HindIII	AAGCTT	1 cut(s)	2273
HinfI	GANTC	8 cut(s)	106, 259, 298, 332, 820, 835, 1157, 1972
HpaII	CCGG	2 cut(s)	147, 1712
HphI	GGTGA	6 cut(s)	37, 281, 935, 1484, 2140, 2204
Hpy166II	GTNNAC	5 cut(s)	46, 144, 716, 1056, 1193
Hpy8I	GTNNAC	5 cut(s)	46, 144, 716, 1056, 1193
Hpy99I	CGWCG	1 cut(s)	41
HpyAV	CCTTC	7 cut(s)	16, 211, 619, 721, 1016, 1032, 2062
HpyCH4IV	ACGT	2 cut(s)	39, 2145
HpyF10VI	GCNNNNNNNGC	5 cut(s)	160, 208, 1751, 1763, 2009
HpySE526I	ACGT	2 cut(s)	39, 2145
Hsp92I	GRCGYC	1 cut(s)	99
HspAI	GCGC	4 cut(s)	99, 1623, 1644, 1924
KasI	GGCGCC	1 cut(s)	98
Ksp22I	TGATCA	2 cut(s)	132, 925
LmnI	GCTCC	2 cut(s)	1009, 1396
Lsp1109I	GCAGC	9 cut(s)	52, 74, 373, 486, 809, 988, 994, 1584, 1741
LweI	GCATC	4 cut(s)	874, 1506, 1594, 2101
MaeI	CTAG	6 cut(s)	1188, 1346, 1773, 1919, 2009, 2201
MaeII	ACGT	2 cut(s)	39, 2145
MaeIII	GTNAC	5 cut(s)	427, 449, 1454, 2051, 2176
MflI	RGATCY	5 cut(s)	177, 538, 1381, 1498, 1846
MhlI	GDGCHC	4 cut(s)	718, 954, 1195, 1414
MlsI	TGGCCA	1 cut(s)	2234
MluNI	TGGCCA	1 cut(s)	2234
Mly113I	GGCGCC	1 cut(s)	99
MlyI	GAGTC	3 cut(s)	341, 829, 1981
Mox20I	TGGCCA	1 cut(s)	2234
Mph1103I	ATGCAT	3 cut(s)	889, 1609, 1828
MroXI	GAANNNNTTC	1 cut(s)	1262
MscI	TGGCCA	1 cut(s)	2234
MseI	TTAA	8 cut(s)	521, 860, 1403, 1539, 1658, 1941, 1968, 2150
MslI	CAYNNNNRTG	5 cut(s)	828, 1788, 1855, 2027, 2223
Msp20I	TGGCCA	1 cut(s)	2234
MspA1I	CMGCKG	1 cut(s)	65
MspI	CCGG	2 cut(s)	147, 1712
MspR9I	CCNGG	7 cut(s)	735, 791, 840, 920, 1113, 1230, 1712
Mva1269I	GAATGC	1 cut(s)	456
MvaI	CCWGG	6 cut(s)	735, 791, 840, 920, 1113, 1230
MwoI	GCNNNNNNNGC	5 cut(s)	160, 208, 1751, 1763, 2009
NarI	GGCGCC	1 cut(s)	99
NciI	CCSGG	1 cut(s)	1712
NdeI	CATATG	1 cut(s)	825
NheI	GCTAGC	1 cut(s)	2008
NlaIV	GGNNCC	8 cut(s)	100, 212, 732, 774, 951, 1383, 1500, 1701
NmuCI	GTSAC	2 cut(s)	2051, 2176
NsbI	TGCGCA	2 cut(s)	1624, 1925
NsiI	ATGCAT	3 cut(s)	889, 1609, 1828
NspI	RCATGY	4 cut(s)	366, 891, 1611, 1723
NspV	TTCGAA	1 cut(s)	805
PaeI	GCATGC	3 cut(s)	891, 1611, 1723
PaeR7I	CTCGAG	1 cut(s)	1415
PagI	TCATGA	2 cut(s)	583, 1648
PaqCI	CACCTGC	1 cut(s)	2017
PcsI	WCGNNNNNNNCGW	1 cut(s)	45
PctI	GAATGC	1 cut(s)	456
PdmI	GAANNNNTTC	1 cut(s)	1262
PfeI	GAWTC	5 cut(s)	106, 259, 298, 820, 1157
PfoI	TCCNGGA	1 cut(s)	838
PleI	GAGTC	3 cut(s)	340, 829, 1980
PluTI	GGCGCC	1 cut(s)	102
PpsI	GAGTC	3 cut(s)	340, 829, 1980
PpuMI	RGGWCCY	1 cut(s)	730
PshBI	ATTAAT	1 cut(s)	1403
PsiI	TTATAA	1 cut(s)	1137
Psp5II	RGGWCCY	1 cut(s)	730
Psp6I	CCWGG	6 cut(s)	733, 789, 838, 918, 1111, 1228
PspFI	CCCAGC	3 cut(s)	369, 1468, 2012
PspGI	CCWGG	6 cut(s)	733, 789, 838, 918, 1111, 1228
PspN4I	GGNNCC	8 cut(s)	100, 212, 732, 774, 951, 1383, 1500, 1701
PspPI	GGNCC	4 cut(s)	144, 730, 935, 1028
PspPPI	RGGWCCY	1 cut(s)	730
PspXI	VCTCGAGB	1 cut(s)	1415
PstI	CTGCAG	4 cut(s)	476, 1036, 1759, 1900
PstNI	CAGNNNCTG	2 cut(s)	256, 1686
PsuI	RGATCY	5 cut(s)	177, 538, 1381, 1498, 1846
PvuII	CAGCTG	1 cut(s)	65
RsaI	GTAC	9 cut(s)	7, 170, 367, 816, 1281, 1960, 2018, 2085, 2144
RsaNI	GTAC	9 cut(s)	6, 169, 366, 815, 1280, 1959, 2017, 2084, 2143
RseI	CAYNNNNRTG	5 cut(s)	828, 1788, 1855, 2027, 2223
SaqAI	TTAA	8 cut(s)	521, 860, 1403, 1539, 1658, 1941, 1968, 2150
Sau96I	GGNCC	4 cut(s)	144, 730, 935, 1028
SbfI	CCTGCAGG	1 cut(s)	1036
ScaI	AGTACT	1 cut(s)	1960
SchI	GAGTC	3 cut(s)	341, 829, 1981
ScrFI	CCNGG	7 cut(s)	735, 791, 840, 920, 1113, 1230, 1712
SdaI	CCTGCAGG	1 cut(s)	1036
SduI	GDGCHC	4 cut(s)	718, 954, 1195, 1414
SfaNI	GCATC	4 cut(s)	874, 1506, 1594, 2101
SfcI	CTRYAG	4 cut(s)	472, 1032, 1755, 1896
SfoI	GGCGCC	1 cut(s)	100
Sfr274I	CTCGAG	1 cut(s)	1415
SfuI	TTCGAA	1 cut(s)	805
SinI	GGWCC	4 cut(s)	144, 730, 935, 1028
SlaI	CTCGAG	1 cut(s)	1415
SmiMI	CAYNNNNRTG	5 cut(s)	828, 1788, 1855, 2027, 2223
SmlI	CTYRAG	6 cut(s)	182, 239, 680, 1415, 1946, 2267
SmoI	CTYRAG	6 cut(s)	182, 239, 680, 1415, 1946, 2267
SphI	GCATGC	3 cut(s)	891, 1611, 1723
Sse8387I	CCTGCAGG	1 cut(s)	1036
SsiI	CCGC	5 cut(s)	892, 1014, 1283, 2170, 2287
SspDI	GGCGCC	1 cut(s)	98
SspI	AATATT	1 cut(s)	993
SspMI	CTAG	6 cut(s)	1188, 1346, 1773, 1919, 2009, 2201
StyD4I	CCNGG	7 cut(s)	733, 789, 838, 918, 1111, 1228, 1710
TaiI	ACGT	2 cut(s)	42, 2148
TaqI	TCGA	6 cut(s)	36, 83, 805, 1416, 1562, 1788
TatI	WGTACW	3 cut(s)	814, 1958, 2083
TauI	GCSGC	1 cut(s)	894
TfiI	GAWTC	5 cut(s)	106, 259, 298, 820, 1157
Tru1I	TTAA	8 cut(s)	521, 860, 1403, 1539, 1658, 1941, 1968, 2150
Tru9I	TTAA	8 cut(s)	521, 860, 1403, 1539, 1658, 1941, 1968, 2150
TseFI	GTSAC	2 cut(s)	2051, 2176
TseI	GCWGC	9 cut(s)	62, 65, 386, 474, 797, 982, 1001, 1597, 1754
Tsp45I	GTSAC	2 cut(s)	2051, 2176
TspDTI	ATGAA	9 cut(s)	89, 668, 734, 740, 812, 1355, 1776, 1857, 2011
TspGWI	ACGGA	3 cut(s)	23, 1268, 2177
VneI	GTGCAC	2 cut(s)	714, 1191
VpaK11BI	GGWCC	4 cut(s)	144, 730, 935, 1028
VspI	ATTAAT	1 cut(s)	1403
XapI	RAATTY	4 cut(s)	692, 756, 909, 2161
XceI	RCATGY	4 cut(s)	366, 891, 1611, 1723
XhoI	CTCGAG	1 cut(s)	1415
XmnI	GAANNNNTTC	1 cut(s)	1262
XspI	CTAG	6 cut(s)	1188, 1346, 1773, 1919, 2009, 2201
ZrmI	AGTACT	1 cut(s)	1960
Zsp2I	ATGCAT	3 cut(s)	889, 1609, 1828

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.