Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0008324

Physical Location & Seq

Genomic Coordinates Forward (+)

33854340 .. 33858825

Gene Structure 4486 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1278600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2193 bp

ATGGGTACGGAGAGGAAGAGGAAGGTCAGCTTGTTCGACGTGGTGGACGAGGCTTCCATCACATCGGCGAAGATGAACAAATCGAACGGCAGCGGAGGAGCTGCTGCCAACCAAAACGCTGTGAACAGTCTTATCAATCGGTGGACCGGGAAGCCGTACTCTCAGAGGTACTATGAGATCCTTGAAAAGAGGAAGACGTTGCCTGTTTGGCACCAGAAAGAGGAGTTCTTGCAAGCTTTCAAGGCCAGCCAGTCTCTCATTTTGGTTGGTGAAACGGGTAGCGGTAAAACCACTCAGATTCCTCAATTTGTTTTGGAAGCTGTCCATTCAGAATCTTCCGATAAACGCAAGAAGATGATGGTTGCGTGTACCCAACCACGTAGGGTGGCTGCAATGTCAGTATCCCGTCGTGTTGCTGAAGAGATGGATGTAACCATTGGAGAAGAAGTTGGTTATAGCATCCGTTTTGAAGACTGCAGCAGTGCCAGAACTGTGTTGAAGTATCTCACCGATGGAATGCTTTTAAGAGAAGCAATGACTGATCCACTTCTAGAAAGATACAGCGTGGTAATTCTTGATGAAGCTCACGAAAGAACTTTAGCCACAGATGTTCTATTTGGGCTTTTGAAGGAAGTGTTGAGAAACAGACCTGATTTGAAGGTGGTTGTGATGAGTGCTACACTTGAAGCTGAAAAGTTTCAGGGTTATTTCAGTGGTGCGCCACTCATGAAAGTTCCTGGAAGGCTTCATCCGGTGGAAATTTTTTACACTGAGGAACCTGAAAGGGATTATCTGGAAGCAGCAATTCGAACAGTTGTGCAGATTCATATGTATGAGACCCCAGGAGATATACTTGTTTTTCTAACAGGAGAGGAGGAGATAGAAGATGCATGCCGCAAAATAAACAAGGAAGTCGGAAATCTGGGTGACCAGGTTGGTCCTGTGAAAGTGGTGCCTCTGTATTCAACTCTACCTCCAGCTATGCAGCAGAAAATATTTGATCCTGCTCCTCCTCCCATAAAAGAAGGTGGAATTCCTGGAAGGAAGATCGTGGTGTCAACAAATATTGCAGAAACTTCTCTGACTATTGATGGAATTGTTTATGTTATTGATCCCGGATTTGCTAAACAAAAAGTCTATAACCCAAGAGTGCGTGTTGAATCCTTGTTGGTATCTCCAATTTCCAAGGCAAGTGCACATCAGAGGTCAGGTCGTGCTGGAAGAACTCAGCCAGGAAAATGTTTCAGACTTTATACTGAGAAAAGTTTCAATAATGATCTTCAACCGCAAACGTATCCAGAAATTTTGAGATCAAACCTTGCAAATACAGTTCTTACTTTGAAGAAACTAGGAATAGATGATCTGGTTCATTTTGATTTTATGGATCCCCCTGCTCCTGAGACATTGATGAGAGCACTAGAGGTCTTGAATTATTTGGGAGCATTGGATGATGAAGGTAACTTGACCAAGCTAGGGGAGATTATGAGTGAGTTCCCGTTGGATCCTCAGATGTCAAAGATGCTCGTAGTCAGCCCTGAGTTCAACTGCTCAAATGAAATTTTGTCAGTATCTGCCATGCTCTCAGTACCCAATTGCTTTGTCCGGCCTAGGGAGGCTCAAAAAGCTGCTGACGAAGCAAAAGCTAGGTTCGGGCACATTGATGGAGATCATCTCACACTTCTGAATGTGTATCATGCATACAAGCAAAATAATGAGGATCCGTCATGGTGCTATGAGAACTTTGTGAATCAGAGGGCATTGAAGTCTGCAGACAACGTCAGGCAGCAGCTAGTGCGCATTATGGCCCGGTTTAACCTCAAGCTATGCAGTACTGAATTCAATAGTCGTGACTACTACATCAACATAAGGAAGGCCATGCTAGCTGGATATTTCATGCAGGTGGCTCATCTGGAACGTACTGGACACTATCTGACAGTGAAAGATAACCAAGTGGTGCATTTGCATCCGTCGAATTGCTTGGACCACAAGCCAGAATGGGTGATCTACAACGAGTATGTTTTGACAAGCAGGAATTTTATCCGTACAGTGACGGATGTTCGTGGGGAGTGGCTGGTCGATATAGCACCCCACTACTATGACTTGGCGAATTTTCCCCAGTGTGAGGCCAAGCGTGTTCTTGAGAAGCTATACAGGAAGCGGGAGGACAAGGATGAGAACAAAAACCGAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0010467 GO:0016020 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

AAA_22 DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

731

Amino Acids

82.82

Weight (kDa)

6.98

Isoelectric Point (pI)

42.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	75 - 229	4e-10	DEAD/DEAH box helicase
AAA_22	PF13401	84 - 224	3.2e-07	AAA domain
Helicase_C	PF00271	270 - 409	8.6e-09	Helicase conserved C-terminal domain
WHD_HA2	PF04408	472 - 500	5.8e-11	Helicase associated domain (HA2), winged-helix
HA2_C	PF21010	501 - 560	4.7e-14	Helicase associated domain (HA2), ratchet-like
OB_NTP_bind	PF07717	622 - 698	1.1e-24	Oligonucleotide/oligosaccharide-binding (OB)-fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008324)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47250 AT3G62310 AT3G62310 AT3G62310
fragaria_vesca	FvH4_1g26860 FvH4_1g26860
malus_domestica	MD09G1286000.v1.1 MD17G1278600.v1.1
prunus_persica	Prupe.3G007400_v2.0.a1
pyrus_communis	pycom09g19330 pycom17g27620 pycom17g27630
rosa_chinensis	RchiOBHm_Chr3g0487101
rosa_laevigata	RLG00000023033
rosa_multiflora	Rmu_co8377811.1_g000001 Rmu_ssc0000271.1_g000001
rosa_roxburghii	Rroxscaffold_6G00395180
rosa_rugosa	Rorug03G0231000
rosa_samantha	Rh3AG279300 Rh3BG315300 Rh3CG313500 Rh3DG310300
rosa_wichuraiana	Rw3G024760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1888
Acc16I	TGCGCA	1 cut(s)	1796
Acc36I	ACCTGC	1 cut(s)	1888
AccB1I	GGYRCC	2 cut(s)	210, 952
AciI	CCGC	5 cut(s)	93, 282, 895, 1286, 2158
AcsI	RAATTY	7 cut(s)	759, 1032, 1302, 1557, 1835, 2032, 2107
AcuI	CTGAAG	1 cut(s)	438
AfaI	GTAC	8 cut(s)	7, 158, 170, 370, 1587, 1831, 1918, 2044
AfiI	CCNNNNNNNGG	4 cut(s)	220, 1473, 1609, 2122
AjiI	CACGTC	1 cut(s)	40
AjnI	CCWGG	5 cut(s)	736, 841, 930, 1036, 1231
Alw21I	GWGCWC	2 cut(s)	1198, 1417
Alw26I	GTCTC	3 cut(s)	258, 830, 1394
Alw44I	GTGCAC	1 cut(s)	1194
AlwNI	CAGNNNCTG	1 cut(s)	1571
AoxI	GGCC	5 cut(s)	243, 1604, 1803, 1872, 2124
ApaLI	GTGCAC	1 cut(s)	1194
ApoI	RAATTY	7 cut(s)	759, 1032, 1302, 1557, 1835, 2032, 2107
Asp700I	GAANNNNTTC	2 cut(s)	696, 1265
AspA2I	CCTAGG	1 cut(s)	1607
AspLEI	GCGC	2 cut(s)	721, 1797
AspS9I	GGNCC	4 cut(s)	144, 938, 1804, 1981
AsuC2I	CCSGG	3 cut(s)	148, 1116, 1807
AsuHPI	GGTGA	4 cut(s)	281, 499, 938, 2011
AsuII	TTCGAA	1 cut(s)	808
AsuNHI	GCTAGC	1 cut(s)	1879
AvaII	GGWCC	3 cut(s)	144, 938, 1981
AvrII	CCTAGG	1 cut(s)	1607
BaeGI	GKGCMC	2 cut(s)	1198, 1656
BamHI	GGATCC	3 cut(s)	1384, 1501, 1717
BanI	GGYRCC	2 cut(s)	210, 952
BbsI	GAAGAC	2 cut(s)	200, 477
Bbv12I	GWGCWC	2 cut(s)	1198, 1417
BccI	CCATC	6 cut(s)	65, 352, 418, 506, 1085, 1655
BceAI	ACGGC	2 cut(s)	103, 139
BciT130I	CCWGG	5 cut(s)	738, 843, 932, 1038, 1233
BciVI	GTATCC	2 cut(s)	412, 1305
BcnI	CCSGG	3 cut(s)	148, 1116, 1807
BcoDI	GTCTC	3 cut(s)	258, 830, 1394
BfaI	CTAG	8 cut(s)	551, 1349, 1418, 1472, 1608, 1644, 1790, 1880
BfmI	CTRYAG	2 cut(s)	475, 1767
BfuAI	ACCTGC	1 cut(s)	1888
BfuI	GTATCC	2 cut(s)	412, 1305
BglI	GCCNNNNNGGC	1 cut(s)	208
BlnI	CCTAGG	1 cut(s)	1607
BmcAI	AGTACT	1 cut(s)	1831
Bme1390I	CCNGG	8 cut(s)	148, 738, 843, 932, 1038, 1116, 1233, 1807
Bme18I	GGWCC	3 cut(s)	144, 938, 1981
BmgBI	CACGTC	1 cut(s)	40
BmgT120I	GGNCC	4 cut(s)	144, 938, 1804, 1981
BmiI	GGNNCC	6 cut(s)	212, 777, 954, 1386, 1503, 1719
BmrFI	CCNGG	8 cut(s)	148, 738, 843, 932, 1038, 1116, 1233, 1807
BmrI	ACTGGG	1 cut(s)	2110
BmsI	GCATC	4 cut(s)	468, 877, 1509, 1972
BmtI	GCTAGC	1 cut(s)	1883
BmuI	ACTGGG	1 cut(s)	2110
BpiI	GAAGAC	2 cut(s)	200, 477
BplI	GAGNNNNNCTC	2 cut(s)	1656, 1688
BpmI	CTGGAG	1 cut(s)	960
Bpu14I	TTCGAA	1 cut(s)	808
BpuEI	CTTGAG	2 cut(s)	1802, 2159
BpuMI	CCSGG	3 cut(s)	148, 1116, 1807
BsaAI	YACGTR	1 cut(s)	380
BsaBI	GATNNNNATC	1 cut(s)	1665
BsaI	GGTCTC	1 cut(s)	830
BsaJI	CCNNGG	3 cut(s)	841, 1185, 1607
BsaWI	WCCGGW	1 cut(s)	751
BsaXI	ACNNNNNCTCC	4 cut(s)	432, 462, 958, 988
Bsc4I	CCNNNNNNNGG	4 cut(s)	220, 1473, 1609, 2122
Bse1I	ACTGG	3 cut(s)	250, 1924, 2116
Bse3DI	GCAATG	2 cut(s)	399, 540
Bse8I	GATNNNNATC	1 cut(s)	1665
BseBI	CCWGG	5 cut(s)	738, 843, 932, 1038, 1233
BseDI	CCNNGG	3 cut(s)	841, 1185, 1607
BseGI	GGATG	7 cut(s)	433, 459, 748, 1453, 1963, 2059, 2176
BseJI	GATNNNNATC	1 cut(s)	1665
BseLI	CCNNNNNNNGG	4 cut(s)	220, 1473, 1609, 2122
BseMI	GCAATG	2 cut(s)	399, 540
BseMII	CTCAG	9 cut(s)	176, 308, 762, 1241, 1248, 1389, 1520, 1527, 1596
BseNI	ACTGG	3 cut(s)	250, 1924, 2116
BseRI	GAGGAG	6 cut(s)	111, 236, 887, 890, 999, 1002
BseSI	GKGCMC	2 cut(s)	1198, 1656
BsgI	GTGCAG	1 cut(s)	839
BshFI	GGCC	5 cut(s)	245, 1606, 1805, 1874, 2126
BshNI	GGYRCC	2 cut(s)	210, 952
BsiHKAI	GWGCWC	2 cut(s)	1198, 1417
BsiSI	CCGG	5 cut(s)	147, 752, 1116, 1603, 1807
BslI	CCNNNNNNNGG	4 cut(s)	220, 1473, 1609, 2122
BsmAI	GTCTC	3 cut(s)	258, 830, 1394
BsmI	GAATGC	1 cut(s)	522
BsnI	GGCC	5 cut(s)	245, 1606, 1805, 1874, 2126
Bso31I	GGTCTC	1 cut(s)	830
Bsp119I	TTCGAA	1 cut(s)	808
Bsp1286I	GDGCHC	3 cut(s)	1198, 1417, 1656
BspACI	CCGC	5 cut(s)	93, 282, 895, 1286, 2158
BspANI	GGCC	5 cut(s)	245, 1606, 1805, 1874, 2126
BspCNI	CTCAG	9 cut(s)	175, 307, 763, 1240, 1249, 1390, 1519, 1528, 1595
BspHI	TCATGA	1 cut(s)	726
BspLI	GGNNCC	6 cut(s)	212, 777, 954, 1386, 1503, 1719
BspMAI	CTGCAG	2 cut(s)	479, 1771
BspMI	ACCTGC	1 cut(s)	1888
BspOI	GCTAGC	1 cut(s)	1883
BspT104I	TTCGAA	1 cut(s)	808
BspT107I	GGYRCC	2 cut(s)	210, 952
BspTNI	GGTCTC	1 cut(s)	830
BsrDI	GCAATG	2 cut(s)	399, 540
BsrI	ACTGG	3 cut(s)	250, 1924, 2116
BssECI	CCNNGG	3 cut(s)	841, 1185, 1607
BssT1I	CCWWGG	2 cut(s)	1185, 1607
Bst2UI	CCWGG	5 cut(s)	738, 843, 932, 1038, 1233
Bst4CI	ACNGT	6 cut(s)	128, 493, 814, 1330, 1936, 2047
Bst6I	CTCTTC	2 cut(s)	11, 414
BstAPI	GCANNNNNTGC	1 cut(s)	1792
BstBAI	YACGTR	1 cut(s)	380
BstBI	TTCGAA	1 cut(s)	808
BstC8I	GCNNGC	4 cut(s)	234, 247, 892, 1881
BstDEI	CTNAG	9 cut(s)	162, 294, 771, 1227, 1257, 1398, 1506, 1536, 1582
BstEII	GGTNACC	1 cut(s)	926
BstF5I	GGATG	7 cut(s)	433, 459, 748, 1453, 1963, 2059, 2176
BstHHI	GCGC	2 cut(s)	721, 1797
BstMAI	GTCTC	3 cut(s)	258, 830, 1394
BstMWI	GCNNNNNNNGC	6 cut(s)	208, 242, 1622, 1634, 1792, 1880
BstNI	CCWGG	5 cut(s)	738, 843, 932, 1038, 1233
BstNSI	RCATGY	1 cut(s)	894
BstPI	GGTNACC	1 cut(s)	926
BstSCI	CCNGG	8 cut(s)	146, 736, 841, 930, 1036, 1114, 1231, 1805
BstSFI	CTRYAG	2 cut(s)	475, 1767
BstSLI	GKGCMC	2 cut(s)	1198, 1656
BstV2I	GAAGAC	2 cut(s)	200, 477
BstX2I	RGATCY	4 cut(s)	177, 1384, 1501, 1717
BstYI	RGATCY	4 cut(s)	177, 1384, 1501, 1717
BsuI	GTATCC	2 cut(s)	412, 1305
BsuRI	GGCC	5 cut(s)	245, 1606, 1805, 1874, 2126
BtrI	CACGTC	1 cut(s)	40
BtsCI	GGATG	7 cut(s)	433, 459, 748, 1453, 1963, 2059, 2176
BtsI	GCAGTG	1 cut(s)	487
BtsIMutI	CAGTG	6 cut(s)	487, 718, 768, 1941, 2052, 2123
BveI	ACCTGC	1 cut(s)	1888
Cac8I	GCNNGC	4 cut(s)	234, 247, 892, 1881
CaiI	CAGNNNCTG	1 cut(s)	1571
CciI	TCATGA	1 cut(s)	726
CfoI	GCGC	2 cut(s)	721, 1797
Cfr13I	GGNCC	4 cut(s)	144, 938, 1804, 1981
CsiI	ACCWGGT	1 cut(s)	930
Csp6I	GTAC	8 cut(s)	6, 157, 169, 369, 1586, 1830, 1917, 2043
CviAII	CATG	7 cut(s)	727, 891, 1576, 1694, 1725, 1876, 1894
CviQI	GTAC	8 cut(s)	6, 157, 169, 369, 1586, 1830, 1917, 2043
DdeI	CTNAG	9 cut(s)	162, 294, 771, 1227, 1257, 1398, 1506, 1536, 1582
Eam1104I	CTCTTC	2 cut(s)	11, 414
EarI	CTCTTC	2 cut(s)	11, 414
Eco130I	CCWWGG	2 cut(s)	1185, 1607
Eco31I	GGTCTC	1 cut(s)	830
Eco47I	GGWCC	3 cut(s)	144, 938, 1981
Eco57I	CTGAAG	1 cut(s)	438
Eco91I	GGTNACC	1 cut(s)	926
EcoO65I	GGTNACC	1 cut(s)	926
EcoRI	GAATTC	2 cut(s)	1032, 1835
EcoRII	CCWGG	5 cut(s)	736, 841, 930, 1036, 1231
EcoT14I	CCWWGG	2 cut(s)	1185, 1607
EcoT22I	ATGCAT	2 cut(s)	892, 1699
ErhI	CCWWGG	2 cut(s)	1185, 1607
FaeI	CATG	7 cut(s)	730, 894, 1579, 1697, 1728, 1879, 1897
FalI	AAGNNNNNCTT	2 cut(s)	14, 46
FatI	CATG	7 cut(s)	726, 890, 1575, 1693, 1724, 1875, 1893
FauI	CCCGC	1 cut(s)	2151
FauNDI	CATATG	1 cut(s)	828
FokI	GGATG	7 cut(s)	440, 446, 735, 1460, 1950, 2066, 2183
FspAI	RTGCGCAY	1 cut(s)	1796
FspBI	CTAG	8 cut(s)	551, 1349, 1418, 1472, 1608, 1644, 1790, 1880
FspI	TGCGCA	1 cut(s)	1796
GlaI	GCGC	2 cut(s)	720, 1796
GsuI	CTGGAG	1 cut(s)	960
HaeIII	GGCC	5 cut(s)	245, 1606, 1805, 1874, 2126
HapII	CCGG	5 cut(s)	147, 752, 1116, 1603, 1807
HhaI	GCGC	2 cut(s)	721, 1797
Hin1II	CATG	7 cut(s)	730, 894, 1579, 1697, 1728, 1879, 1897
Hin6I	GCGC	2 cut(s)	719, 1795
HinP1I	GCGC	2 cut(s)	719, 1795
HincII	GTYRAC	1 cut(s)	1059
HindII	GTYRAC	1 cut(s)	1059
HindIII	AAGCTT	1 cut(s)	234
HinfI	GANTC	5 cut(s)	298, 332, 823, 1160, 1747
HpaII	CCGG	5 cut(s)	147, 752, 1116, 1603, 1807
HphI	GGTGA	4 cut(s)	281, 499, 938, 2011
Hpy166II	GTNNAC	6 cut(s)	46, 124, 144, 369, 1059, 1196
Hpy8I	GTNNAC	6 cut(s)	46, 124, 144, 369, 1059, 1196
Hpy99I	CGWCG	3 cut(s)	41, 411, 1972
HpyAV	CCTTC	8 cut(s)	16, 622, 652, 735, 1019, 1035, 1448, 1864
HpyCH4III	ACNGT	6 cut(s)	128, 493, 814, 1330, 1936, 2047
HpyCH4IV	ACGT	6 cut(s)	39, 197, 379, 1292, 1776, 1915
HpyF10VI	GCNNNNNNNGC	6 cut(s)	208, 242, 1622, 1634, 1792, 1880
HpyF3I	CTNAG	9 cut(s)	162, 294, 771, 1227, 1257, 1398, 1506, 1536, 1582
HpySE526I	ACGT	6 cut(s)	39, 197, 379, 1292, 1776, 1915
Hsp92II	CATG	7 cut(s)	730, 894, 1579, 1697, 1728, 1879, 1897
HspAI	GCGC	2 cut(s)	719, 1795
LmnI	GCTCC	4 cut(s)	98, 1012, 1399, 1439
LweI	GCATC	4 cut(s)	468, 877, 1509, 1972
MabI	ACCWGGT	1 cut(s)	930
MaeI	CTAG	8 cut(s)	551, 1349, 1418, 1472, 1608, 1644, 1790, 1880
MaeII	ACGT	6 cut(s)	39, 197, 379, 1292, 1776, 1915
MaeIII	GTNAC	5 cut(s)	430, 926, 1457, 1847, 2047
MfeI	CAATTG	1 cut(s)	1591
MflI	RGATCY	4 cut(s)	177, 1384, 1501, 1717
MhlI	GDGCHC	3 cut(s)	1198, 1417, 1656
MmeI	TCCRAC	2 cut(s)	895, 1479
Mph1103I	ATGCAT	2 cut(s)	892, 1699
MroXI	GAANNNNTTC	2 cut(s)	696, 1265
MseI	TTAA	2 cut(s)	524, 1812
MslI	CAYNNNNRTG	4 cut(s)	831, 1659, 1898, 2094
MspA1I	CMGCKG	1 cut(s)	93
MspI	CCGG	5 cut(s)	147, 752, 1116, 1603, 1807
MspR9I	CCNGG	8 cut(s)	148, 738, 843, 932, 1038, 1116, 1233, 1807
MunI	CAATTG	1 cut(s)	1591
Mva1269I	GAATGC	1 cut(s)	522
MvaI	CCWGG	5 cut(s)	738, 843, 932, 1038, 1233
MwoI	GCNNNNNNNGC	6 cut(s)	208, 242, 1622, 1634, 1792, 1880
NciI	CCSGG	3 cut(s)	148, 1116, 1807
NdeI	CATATG	1 cut(s)	828
NheI	GCTAGC	1 cut(s)	1879
NlaIII	CATG	7 cut(s)	730, 894, 1579, 1697, 1728, 1879, 1897
NlaIV	GGNNCC	6 cut(s)	212, 777, 954, 1386, 1503, 1719
NmuCI	GTSAC	3 cut(s)	926, 1847, 2047
NsbI	TGCGCA	1 cut(s)	1796
NsiI	ATGCAT	2 cut(s)	892, 1699
NspI	RCATGY	1 cut(s)	894
NspV	TTCGAA	1 cut(s)	808
PaeI	GCATGC	1 cut(s)	894
PagI	TCATGA	1 cut(s)	726
PaqCI	CACCTGC	1 cut(s)	1888
PcsI	WCGNNNNNNNCGW	1 cut(s)	45
PctI	GAATGC	1 cut(s)	522
PdmI	GAANNNNTTC	2 cut(s)	696, 1265
PfeI	GAWTC	5 cut(s)	298, 332, 823, 1160, 1747
PflFI	GACNNNGTC	1 cut(s)	1775
PfoI	TCCNGGA	3 cut(s)	736, 1036, 1114
Ppu21I	YACGTR	1 cut(s)	380
Psp6I	CCWGG	5 cut(s)	736, 841, 930, 1036, 1231
PspEI	GGTNACC	1 cut(s)	926
PspGI	CCWGG	5 cut(s)	736, 841, 930, 1036, 1231
PspN4I	GGNNCC	6 cut(s)	212, 777, 954, 1386, 1503, 1719
PspPI	GGNCC	4 cut(s)	144, 938, 1804, 1981
PstI	CTGCAG	2 cut(s)	479, 1771
PstNI	CAGNNNCTG	1 cut(s)	1571
PsuI	RGATCY	4 cut(s)	177, 1384, 1501, 1717
PsyI	GACNNNGTC	1 cut(s)	1775
RsaI	GTAC	8 cut(s)	7, 158, 170, 370, 1587, 1831, 1918, 2044
RsaNI	GTAC	8 cut(s)	6, 157, 169, 369, 1586, 1830, 1917, 2043
RseI	CAYNNNNRTG	4 cut(s)	831, 1659, 1898, 2094
SaqAI	TTAA	2 cut(s)	524, 1812
Sau96I	GGNCC	4 cut(s)	144, 938, 1804, 1981
ScaI	AGTACT	1 cut(s)	1831
ScrFI	CCNGG	8 cut(s)	148, 738, 843, 932, 1038, 1116, 1233, 1807
SduI	GDGCHC	3 cut(s)	1198, 1417, 1656
SexAI	ACCWGGT	1 cut(s)	930
SfaNI	GCATC	4 cut(s)	468, 877, 1509, 1972
SfcI	CTRYAG	2 cut(s)	475, 1767
SfuI	TTCGAA	1 cut(s)	808
SinI	GGWCC	3 cut(s)	144, 938, 1981
SmiMI	CAYNNNNRTG	4 cut(s)	831, 1659, 1898, 2094
SmlI	CTYRAG	2 cut(s)	1817, 2138
SmoI	CTYRAG	2 cut(s)	1817, 2138
SphI	GCATGC	1 cut(s)	894
SsiI	CCGC	5 cut(s)	93, 282, 895, 1286, 2158
SspI	AATATT	2 cut(s)	996, 1066
SspMI	CTAG	8 cut(s)	551, 1349, 1418, 1472, 1608, 1644, 1790, 1880
StyD4I	CCNGG	8 cut(s)	146, 736, 841, 930, 1036, 1114, 1231, 1805
StyI	CCWWGG	2 cut(s)	1185, 1607
TaaI	ACNGT	6 cut(s)	128, 493, 814, 1330, 1936, 2047
TaiI	ACGT	6 cut(s)	42, 200, 382, 1295, 1779, 1918
TaqI	TCGA	5 cut(s)	36, 83, 808, 1970, 2076
TatI	WGTACW	1 cut(s)	1829
TauI	GCSGC	1 cut(s)	897
TfiI	GAWTC	5 cut(s)	298, 332, 823, 1160, 1747
Tru1I	TTAA	2 cut(s)	524, 1812
Tru9I	TTAA	2 cut(s)	524, 1812
TscAI	CASTG	6 cut(s)	487, 718, 775, 1941, 2052, 2123
TseFI	GTSAC	3 cut(s)	926, 1847, 2047
Tsp45I	GTSAC	3 cut(s)	926, 1847, 2047
TspDTI	ATGAA	9 cut(s)	89, 594, 737, 743, 815, 1358, 1467, 1569, 1882
TspGWI	ACGGA	6 cut(s)	23, 452, 1710, 1956, 2030, 2066
TspRI	CASTG	6 cut(s)	487, 718, 775, 1941, 2052, 2123
Tth111I	GACNNNGTC	1 cut(s)	1775
VneI	GTGCAC	1 cut(s)	1194
VpaK11BI	GGWCC	3 cut(s)	144, 938, 1981
XapI	RAATTY	7 cut(s)	759, 1032, 1302, 1557, 1835, 2032, 2107
XbaI	TCTAGA	1 cut(s)	550
XceI	RCATGY	1 cut(s)	894
XmaJI	CCTAGG	1 cut(s)	1607
XmnI	GAANNNNTTC	2 cut(s)	696, 1265
XspI	CTAG	8 cut(s)	551, 1349, 1418, 1472, 1608, 1644, 1790, 1880
ZrmI	AGTACT	1 cut(s)	1831
Zsp2I	ATGCAT	2 cut(s)	892, 1699

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.