FvH4_1g27060

Nodal modulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0011945
Physical Location & Seq
Reverse (-)
18835645 .. 18846952
11308 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g27060.t2

Sequence Viewer

Length: 3600 bp
ATGAAAGATGCCCGCTCACTCCTACTAGTCCTCCTCGCCGTGACGTCACTGCTCTCCACCGCCGCCTTCGCCGATTCAATCCACGGCTGCGGCGGCTTCGTCGAGGCGAGCTCTGCTTTGATCAAGGCCAGGAAGGCCTCCGATGCTAAATTGGACTATTCTCACATCACGGTTGAGCTTCGAACTGTTGATGGATTGCTCAAAGAGAGTACTCAGTGTGCTCCCAATGGCTACTACTTCATCCCCGTCTACGACAAGGGCTCTTTTGTGATTAAAATCAACGGACCCCAAGGATGGTCATTGCACCCTGATAAGGTTCCGGTCGTTGTTGATAACACTGGCTGCAATGGCAGTGAAGATATTAATTTCCGGTTTACAGGGTTCTCCATTTCTGGTAGAGTTCTGGGAGCTGTTGGTGGAGAGAGCTGTGCTCTTCAAGATGGAGGTCCCTCAGACATCAAAGTCGAACTTTTGTCAGACAGTGGTGATGTTGTTTCTTCTGTTTCAACTTCGTCAGGGGGAAGCTTCTTGTTCAAGAATATTATTCCAGGAAAATATGAAATACGTGCTTCGCATCCTGACTTGAAAGTTGAAATAAGAGGTTCAACAGAGGTGAATGTGGGATTTGGTAACGGTGTAGTTGATGACATTTTCTTTGTTCCTGGCTATGACATAAGCGGATTTGTTGTTTCTCAGGGGAATCCAATATTAGGAGTTCATGTTTATTTACACTCAGATGATGTCTTAGAGGTAAATTGTCCCCAAGGTTCTGGTACAGGCTCTGAAATGAAGAAGGCTCTTTGCCACGCCATATCTGATGCACATGGAAAATTCATGTTCAAGTCGCTACCTTGCGGAACTTATGAGCTAATACCTTACTACAAGGGAGAAAACACGGTCTTTGATGTTTCACCTCCCGTCATGTCAGTGACTGTTGAACATCAACATGTGACAGTTCCGCAAACATTCCAGGTCACTGGATTTTCTGTTGGAGGGCGTGTCGTTGACGGAAACAACATGGGTGTTGAAGGTGTTAAGATTATAGTTGATGGTCATGAAAGGTCCATCACCGACAAACAAGGTTATTACAAGCTTGACCAGGTTACTTCCAATCGCTATACAATAGAAGCCACAAAGGAGCATTACAAGTTCAGTAATCTGAAGGATTATTTGGTATTGCCGAACATGGCTTCTGTTGTGGACATTAAAGCAGTTTCCTATGGTGTCTGCGGTGTGGTGCAGATGGTTAGTGCTGGTTACAAGGCAAAGGTGGCTTTGACTCATGGACCTGAAAATGTCAAACCACAAGTGAAGCAAACCAATGGAAATGGAAATTTCTGCTTCGAGGTTCCAACTGGTGAATATCGTTTATCTGCTTTGGCACCTGAGAGTGCTTCTGGTATACTGTTTGTGCCATCTCATATTGATGTTGTGGTCAAAAGTCCATTATTGAACGTCAAATTTTCTCAGGCACTAGTTACAGTACGCGGAACTGTAGTCTGCAAAGAGAAGTGTGGCACATCTGTTTCTGTTGCCCTTAGCAGTATAGGTGGTAAGCGTAATGAAAAGACAGAGACAATTAGTTTGACTGATGAGAGTAGTGAATTTCTCTTTCATGACGTCATCCCTGGAAAATACAGAGTTGAGGTTAAACGCAACTCCCGGGAATCTGTTAATGGAGAAGATAATTGGTGCTGGAAGCAAAGCTCGATTGATGTGGATGTTGGTGTTGATGACGTAAAAGGAATTGAATTTGTCCAGAAAGGTTACTGGATCCGTTTGATTTCCACCCATGATGTAGATGCTTCTATGATTCATCCAGATGGTTCATCCATGGATTTAAAAATTAAGAAGGGTTCCCAGAATATATGTGTGGAACATCCTGGAGTACACGAACTTCTTTTTGTCAACTCTTGTATTTTCTTTGGGAGCTCATCAATTAAGATTGATACATCAAACCCATCGCCTATTCATCTAAAAGGAGAGAAGTACCTTCTTAAAGGACAAATCAATGTTGCATCAAGCTCATCTGATGGTGTGCACAAGTTATCTGAGAATTTTATTGTAGACATTGTGAATTCTGAGGGCAGTATTATAGATAGTACAACAGCTGGACTTGCTCCCATTGGAAACGAGCAAACAAGTTATTCTGTTTATGAGTTCTCTGTATGGGCCAAACTTGGAGAAAAACTTATCTTTGTTCCTCGGGATGCAAGAAATAATGACATGGGGAAGATATTGTTTTATCCCAGACAACATTATGTTGTGGTTAATAATGATGGTTGTCAAGCTATGATCCCTACATTTGATGGTCGATTAGGTTTATATATTAAAGGATCAGTGTCTCCCCCTCTTTCTGATGTTCATATTAAGATCATTGCTGCTGGAGATAGCCACATTGCACAACTTAAGGAAGGGGAATTAGTTGTTGAAACTGCCACAGCAACAGATGGCTCATTTGTTGGGGGGCCTTTATATGATGATATAACATACAATGTTGAGGCTTCAAAGCTTGGCTACCATTTAAAACAAGTTGGTCCTCATTCCTTTTCCTGTCAGAAGCTTGGCCAAATTGCTGTAGATATATATTCTAAAGATGACGCCAGAGAGCTCATTCCTTCTGTGTTGTTATCTTTGAGTGGCGATGATGGCTACAGAAACAACTCGGTATCTGGAGCTGGTGGAGCATTCCTCTTTAGTAACTTATTTCCTGGAACTTTCTATTTACGTCCTCTGCTAAAGGAGTATGCCTTCTCTCCTCCATCACAGGCTATTGATCTTGGCTCCGGGGAGTCTAAAGAAGCTATTTTCCAGGCCACTCGCGTTGCATACAGTGCCATGGGTGTGGTCGCTTTGTTGTCTGGCCAACCAAAAGAAGGTGTGTTAATTGAGGCCAGATCAGAATCAAAAGGCTTCTATGAGGAAACAGTGACTGACTCATCTGGAAGTTATCGTTTGAGAGGACTTCTTCCTGACACAACCTATGTAATCAAAGTAGTACAAAGGGATGGGTTGGGCAGCAGTGAAATTGAGAGGGCATCACCTGACTCTGTTCCGGTTAAGGTTGGGTACGAAGATATCAAAGGATTAGATTTTCTGGTGTTTGAACAGCCAGACAAAACTATTTTAAGTTGCCATGTCGAAGGAAAGAGAAATGAGGAACTGCACTCGCATTTACTGGTGGAAATCAAATCATCTGGTGAAAATCCTAAGATTCAATCCGTCTTCCCACTTCCGCTTTCAAACTTTTTCCAGGTGAAGGACTTACCCAAGGGTAAGCACCTTCTGCAACTCCGGTCTAGTCTTCCATCTAGCAGCCACAAATTTGAATCCGAAATTATTGAGGTCGATTTAGAGAAGAATGCTCATATTCATGTTGGCCCACTCAAATACAGTTTTGAAGAGGATCACCAGAAGCAGGATTTGACTCCAGCTCCGGTTTTTCCTCTTATCGTAGGAGTTTCAGTGATTGCTCTATTTATTAGTATCCCAAGGTTGAACGATTTGTATCAATCCATGATTGGAACACCAACACCTGGGTTCACTACAACCGCAAAGAAGGAAGTGAGGAAGCCCATGTTGAGAAAAAAGACGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CarboxypepD_reg

Protein Analysis

1200

Amino Acids

130.14

Weight (kDa)

5.86

Isoelectric Point (pI)

31.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NOMO1-like_1st PF22898 46 - 123 8.5e-32 BOS complex subunit NOMO1-like, first beta sandwich domain
NOMO1-like_2nd PF22904 125 - 218 1.1e-18 BOS complex subunit NOMO1-like, second beta sandwich domain
NOMO_3rd PF23193 263 - 326 1.5e-10 NOMO third transthyretin-like domain
NOMO1-like_2nd PF22904 326 - 387 3.6e-07 BOS complex subunit NOMO1-like, second beta sandwich domain
CarboxypepD_reg PF13620 329 - 402 4.6e-07 Carboxypeptidase regulatory-like domain
NOMO_5th PF23194 409 - 488 2.8e-08 NOMO fifth transthyretin-like domain
NOMO_6th PF23196 499 - 570 1e-06 NOMO sixth transthyretin-like domain
Ig_NOMO PF23141 591 - 663 6.4e-27 NOMO Ig-like domain
NOMO_8th PF23660 665 - 772 1.3e-39 NOMO eighth prealbumin-like domain
NOMO1-like_9th PF22902 774 - 846 1.7e-13 BOS complex subunit NOMO1-like, beta sandwich domain
CarboxypepD_reg PF13620 948 - 1009 4.2e-07 Carboxypeptidase regulatory-like domain
DUF7152 PF23662 1040 - 1138 4.2e-46 Domain of unknown function (DUF7152)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011945)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G62360 AT3G62360
fragaria_vesca FvH4_1g27060
malus_domestica MD09G1284300.v1.1 MD17G1277500.v1.1
prunus_persica Prupe.3G010500_v2.0.a1 Prupe.3G010500_v2.0.a1 Prupe.3G010500_v2.0.a1
pyrus_communis pycom17g27510
rosa_chinensis RchiOBHm_Chr3g0487381
rosa_laevigata RLG00000023009
rosa_multiflora Rmu_sc0001955.1_g000004
rosa_roxburghii Rroxscaffold_6G00394840
rosa_rugosa Rorug03G0233600
rosa_samantha Rh3AG281600 Rh3BG317200 Rh3CG315300 Rh3DG313200
rosa_wichuraiana Rw3G024930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 461
AatII GACGTC 2 cut(s) 47, 1623
AccB1I GGYRCC 1 cut(s) 1381
AccB7I CCANNNNNTGG 1 cut(s) 3536
AccBSI CCGCTC 1 cut(s) 15
AccI GTMKAC 3 cut(s) 249, 1402, 2067
AccII CGCG 2 cut(s) 1488, 2823
AclWI GGATC 5 cut(s) 1768, 1781, 2290, 2344, 3414
AcoI YGGCCR 2 cut(s) 2566, 2863
AcsI RAATTY 8 cut(s) 830, 1333, 1460, 1604, 1751, 2056, 2077, 3323
AcuI CTGAAG 1 cut(s) 1181
AcyI GRCGYC 3 cut(s) 44, 1620, 2601
AfaI GTAC 9 cut(s) 211, 775, 1485, 1890, 1991, 2104, 3000, 3071, 3596
AfiI CCNNNNNNNGG 7 cut(s) 294, 313, 710, 2876, 3259, 3418, 3536
AflII CTTAAG 1 cut(s) 2408
AflIII ACRYGT 1 cut(s) 946
AhlI ACTAGT 2 cut(s) 25, 1474
AjuI GAANNNNNNNTTGG 2 cut(s) 1154, 1186
Alw21I GWGCWC 6 cut(s) 113, 223, 433, 1934, 2043, 2613
Alw26I GTCTC 2 cut(s) 1568, 2349
Alw44I GTGCAC 1 cut(s) 2039
AlwI GGATC 5 cut(s) 1768, 1781, 2290, 2344, 3414
AlwNI CAGNNNCTG 3 cut(s) 782, 932, 2933
Ama87I CYCGRG 2 cut(s) 1662, 2205
AoxI GGCC 9 cut(s) 126, 135, 2172, 2468, 2566, 2814, 2863, 2892, 3379
ApaLI GTGCAC 1 cut(s) 2039
ApeKI GCWGC 5 cut(s) 87, 342, 2381, 3018, 3315
ApoI RAATTY 8 cut(s) 830, 1333, 1460, 1604, 1751, 2056, 2077, 3323
ArsI GACNNNNNNTTYG 4 cut(s) 638, 670, 1567, 1599
AseI ATTAAT 1 cut(s) 363
AspS9I GGNCC 8 cut(s) 284, 446, 1062, 1286, 2172, 2468, 2537, 3380
AsuC2I CCSGG 3 cut(s) 1663, 1664, 2788
AsuHPI GGTGA 9 cut(s) 497, 625, 903, 1060, 1370, 3033, 3212, 3268, 3401
AsuII TTCGAA 1 cut(s) 181
AvaI CYCGRG 2 cut(s) 1662, 2205
AvaII GGWCC 5 cut(s) 284, 446, 1062, 1286, 2537
BaeGI GKGCMC 1 cut(s) 2043
BalI TGGCCA 2 cut(s) 2568, 2865
BamHI GGATCC 1 cut(s) 1773
BanI GGYRCC 1 cut(s) 1381
BanII GRGCYC 4 cut(s) 113, 263, 1934, 2613
BarI GAAGNNNNNNTAC 2 cut(s) 1881, 1913
BbsI GAAGAC 2 cut(s) 3217, 3296
Bbv12I GWGCWC 6 cut(s) 113, 223, 433, 1934, 2043, 2613
BbvI GCAGC 5 cut(s) 74, 329, 2368, 3030, 3327
BceAI ACGGC 2 cut(s) 23, 100
BciVI GTATCC 1 cut(s) 3497
BclI TGATCA 1 cut(s) 120
BcnI CCSGG 3 cut(s) 1663, 1664, 2788
BcoDI GTCTC 2 cut(s) 1568, 2349
BcuI ACTAGT 2 cut(s) 25, 1474
BfaI CTAG 4 cut(s) 26, 1475, 3300, 3312
BfmI CTRYAG 3 cut(s) 1494, 2577, 2653
BfrI CTTAAG 1 cut(s) 2408
BfuI GTATCC 1 cut(s) 3497
BglI GCCNNNNNGGC 1 cut(s) 134
BisI GCNGC 8 cut(s) 63, 88, 91, 94, 343, 2382, 3019, 3316
BlsI GCNGC 8 cut(s) 64, 89, 92, 95, 344, 2383, 3020, 3317
BmcAI AGTACT 1 cut(s) 211
Bme18I GGWCC 5 cut(s) 284, 446, 1062, 1286, 2537
BmeT110I CYCGRG 2 cut(s) 1662, 2205
BmgT120I GGNCC 8 cut(s) 284, 446, 1062, 1286, 2172, 2468, 2537, 3380
BmiI GGNNCC 9 cut(s) 286, 318, 448, 1350, 1383, 1775, 1858, 2469, 2785
BmsI GCATC 7 cut(s) 133, 583, 808, 1792, 2027, 2200, 3047
BpiI GAAGAC 2 cut(s) 3217, 3296
BplI GAGNNNNNCTC 6 cut(s) 95, 127, 415, 447, 2676, 2708
BpmI CTGGAG 4 cut(s) 1905, 2406, 2694, 3414
Bpu10I CCTNAGC 1 cut(s) 1538
Bpu14I TTCGAA 1 cut(s) 181
BpuMI CCSGG 3 cut(s) 1663, 1664, 2788
BsaAI YACGTR 1 cut(s) 566
BsaBI GATNNNNATC 3 cut(s) 275, 2902, 3507
BsaHI GRCGYC 3 cut(s) 44, 1620, 2601
BsaWI WCCGGW 5 cut(s) 319, 369, 3055, 3294, 3436
Bsc4I CCNNNNNNNGG 7 cut(s) 294, 313, 710, 2876, 3259, 3418, 3536
Bse1I ACTGG 5 cut(s) 343, 982, 1360, 1775, 3183
Bse3DI GCAATG 4 cut(s) 299, 352, 2376, 2397
Bse8I GATNNNNATC 3 cut(s) 275, 2902, 3507
BseJI GATNNNNATC 3 cut(s) 275, 2902, 3507
BseLI CCNNNNNNNGG 7 cut(s) 294, 313, 710, 2876, 3259, 3418, 3536
BseMI GCAATG 4 cut(s) 299, 352, 2376, 2397
BseMII CTCAG 8 cut(s) 227, 465, 707, 747, 1377, 1481, 2043, 2073
BseNI ACTGG 5 cut(s) 343, 982, 1360, 1775, 3183
BseRI GAGGAG 2 cut(s) 23, 2748
BseSI GKGCMC 1 cut(s) 2043
BseXI GCAGC 5 cut(s) 74, 329, 2368, 3030, 3327
BsgI GTGCAG 2 cut(s) 1259, 3149
Bsh1236I CGCG 2 cut(s) 1488, 2823
Bsh1285I CGRYCG 1 cut(s) 324
BshFI GGCC 9 cut(s) 128, 137, 2174, 2470, 2568, 2816, 2865, 2894, 3381
BshNI GGYRCC 1 cut(s) 1381
BsiEI CGRYCG 1 cut(s) 324
BsiHKAI GWGCWC 6 cut(s) 113, 223, 433, 1934, 2043, 2613
BsiHKCI CYCGRG 2 cut(s) 1662, 2205
BsiSI CCGG 7 cut(s) 320, 370, 1663, 2787, 3056, 3295, 3437
BslFI GGGAC 2 cut(s) 432, 744
BslI CCNNNNNNNGG 7 cut(s) 294, 313, 710, 2876, 3259, 3418, 3536
BsmAI GTCTC 2 cut(s) 1568, 2349
BsmFI GGGAC 2 cut(s) 432, 744
BsmI GAATGC 2 cut(s) 2687, 3367
BsnI GGCC 9 cut(s) 128, 137, 2174, 2470, 2568, 2816, 2865, 2894, 3381
BsoBI CYCGRG 2 cut(s) 1662, 2205
Bsp119I TTCGAA 1 cut(s) 181
Bsp1286I GDGCHC 7 cut(s) 113, 223, 263, 433, 1934, 2043, 2613
Bsp143I GATC 8 cut(s) 120, 1773, 2295, 2336, 2373, 2776, 2897, 3406
Bsp19I CCATGG 2 cut(s) 1833, 2838
BspANI GGCC 9 cut(s) 128, 137, 2174, 2470, 2568, 2816, 2865, 2894, 3381
BspCNI CTCAG 8 cut(s) 226, 464, 706, 746, 1378, 1480, 2044, 2074
BspFNI CGCG 2 cut(s) 1488, 2823
BspHI TCATGA 2 cut(s) 1054, 1615
BspLI GGNNCC 9 cut(s) 286, 318, 448, 1350, 1383, 1775, 1858, 2469, 2785
BspPI GGATC 5 cut(s) 1768, 1781, 2290, 2344, 3414
BspQI GCTCTTC 1 cut(s) 438
BspT104I TTCGAA 1 cut(s) 181
BspT107I GGYRCC 1 cut(s) 1381
BspTI CTTAAG 1 cut(s) 2408
BsrBI CCGCTC 1 cut(s) 15
BsrDI GCAATG 4 cut(s) 299, 352, 2376, 2397
BsrI ACTGG 5 cut(s) 343, 982, 1360, 1775, 3183
BssMI GATC 8 cut(s) 120, 1773, 2295, 2336, 2373, 2776, 2897, 3406
BssNAI GTATAC 1 cut(s) 1403
BssNI GRCGYC 3 cut(s) 44, 1620, 2601
BssT1I CCWWGG 6 cut(s) 289, 763, 1833, 2838, 3270, 3491
Bst1107I GTATAC 1 cut(s) 1403
Bst6I CTCTTC 2 cut(s) 438, 3396
BstACI GRCGYC 3 cut(s) 44, 1620, 2601
BstAFI CTTAAG 1 cut(s) 2408
BstAPI GCANNNNNTGC 2 cut(s) 2834, 3286
BstBAI YACGTR 1 cut(s) 566
BstBI TTCGAA 1 cut(s) 181
BstC8I GCNNGC 2 cut(s) 13, 109
BstDSI CCRYGG 3 cut(s) 82, 1833, 2838
BstFNI CGCG 2 cut(s) 1488, 2823
BstKTI GATC 8 cut(s) 123, 1776, 2298, 2339, 2376, 2779, 2900, 3409
BstMAI GTCTC 2 cut(s) 1568, 2349
BstMBI GATC 8 cut(s) 120, 1773, 2295, 2336, 2373, 2776, 2897, 3406
BstMCI CGRYCG 1 cut(s) 324
BstNSI RCATGY 1 cut(s) 950
BstSFI CTRYAG 3 cut(s) 1494, 2577, 2653
BstSLI GKGCMC 1 cut(s) 2043
BstUI CGCG 2 cut(s) 1488, 2823
BstV1I GCAGC 5 cut(s) 74, 329, 2368, 3030, 3327
BstV2I GAAGAC 2 cut(s) 3217, 3296
BstX2I RGATCY 1 cut(s) 1773
BstXI CCANNNNNNTGG 3 cut(s) 770, 977, 2845
BstYI RGATCY 1 cut(s) 1773
BstZ17I GTATAC 1 cut(s) 1403
BsuI GTATCC 1 cut(s) 3497
BsuRI GGCC 9 cut(s) 128, 137, 2174, 2470, 2568, 2816, 2865, 2894, 3381
BtgI CCRYGG 3 cut(s) 82, 1833, 2838
BtgZI GCGATG 2 cut(s) 1947, 2658
BtsI GCAGTG 3 cut(s) 47, 358, 3028
Cac8I GCNNGC 2 cut(s) 13, 109
CaiI CAGNNNCTG 3 cut(s) 782, 932, 2933
CciI TCATGA 2 cut(s) 1054, 1615
Cfr13I GGNCC 8 cut(s) 284, 446, 1062, 1286, 2172, 2468, 2537, 3380
Cfr9I CCCGGG 1 cut(s) 1662
CseI GACGC 1 cut(s) 2609
CsiI ACCWGGT 1 cut(s) 1098
Csp6I GTAC 9 cut(s) 210, 774, 1484, 1889, 1990, 2103, 2999, 3070, 3595
CspCI CAANNNNNGTGG 2 cut(s) 2806, 2841
CviQI GTAC 9 cut(s) 210, 774, 1484, 1889, 1990, 2103, 2999, 3070, 3595
DpnI GATC 8 cut(s) 122, 1775, 2297, 2338, 2375, 2778, 2899, 3408
DpnII GATC 8 cut(s) 120, 1773, 2295, 2336, 2373, 2776, 2897, 3406
DraI TTTAAA 2 cut(s) 1842, 2526
DrdI GACNNNNNNGTC 1 cut(s) 461
DseDI GACNNNNNNGTC 1 cut(s) 461
EaeI YGGCCR 2 cut(s) 2566, 2863
Eam1104I CTCTTC 2 cut(s) 438, 3396
EarI CTCTTC 2 cut(s) 438, 3396
Ecl136II GAGCTC 3 cut(s) 111, 1932, 2611
Eco130I CCWWGG 6 cut(s) 289, 763, 1833, 2838, 3270, 3491
Eco147I AGGCCT 1 cut(s) 137
Eco24I GRGCYC 4 cut(s) 113, 263, 1934, 2613
Eco32I GATATC 1 cut(s) 3079
Eco47I GGWCC 5 cut(s) 284, 446, 1062, 1286, 2537
Eco53kI GAGCTC 3 cut(s) 111, 1932, 2611
Eco57I CTGAAG 1 cut(s) 1181
Eco88I CYCGRG 2 cut(s) 1662, 2205
EcoICRI GAGCTC 3 cut(s) 111, 1932, 2611
EcoO109I RGGNCCY 2 cut(s) 446, 2468
EcoRI GAATTC 1 cut(s) 2077
EcoRV GATATC 1 cut(s) 3079
EcoT14I CCWWGG 6 cut(s) 289, 763, 1833, 2838, 3270, 3491
EcoT38I GRGCYC 4 cut(s) 113, 263, 1934, 2613
ErhI CCWWGG 6 cut(s) 289, 763, 1833, 2838, 3270, 3491
FalI AAGNNNNNCTT 2 cut(s) 453, 485
FaqI GGGAC 2 cut(s) 432, 744
FauI CCCGC 1 cut(s) 20
FbaI TGATCA 1 cut(s) 120
FblI GTMKAC 3 cut(s) 249, 1402, 2067
Fnu4HI GCNGC 8 cut(s) 63, 88, 91, 94, 343, 2382, 3019, 3316
FriOI GRGCYC 4 cut(s) 113, 263, 1934, 2613
Fsp4HI GCNGC 8 cut(s) 63, 88, 91, 94, 343, 2382, 3019, 3316
FspBI CTAG 4 cut(s) 26, 1475, 3300, 3312
GluI GCNGC 8 cut(s) 63, 88, 91, 94, 343, 2382, 3019, 3316
GsuI CTGGAG 4 cut(s) 1905, 2406, 2694, 3414
HaeIII GGCC 9 cut(s) 128, 137, 2174, 2470, 2568, 2816, 2865, 2894, 3381
HapII CCGG 7 cut(s) 320, 370, 1663, 2787, 3056, 3295, 3437
HgaI GACGC 1 cut(s) 2609
Hin1I GRCGYC 3 cut(s) 44, 1620, 2601
HincII GTYRAC 2 cut(s) 1006, 1909
HindII GTYRAC 2 cut(s) 1006, 1909
HindIII AAGCTT 4 cut(s) 523, 1091, 2510, 2561
HpaII CCGG 7 cut(s) 320, 370, 1663, 2787, 3056, 3295, 3437
HphI GGTGA 9 cut(s) 497, 625, 903, 1060, 1370, 3033, 3212, 3268, 3401
Hpy99I CGWCG 1 cut(s) 104
HpyCH4IV ACGT 7 cut(s) 44, 565, 1455, 1620, 1737, 2728, 3593
HpySE526I ACGT 7 cut(s) 44, 565, 1455, 1620, 1737, 2728, 3593
Hsp92I GRCGYC 3 cut(s) 44, 1620, 2601
Ksp22I TGATCA 1 cut(s) 120
Kzo9I GATC 8 cut(s) 120, 1773, 2295, 2336, 2373, 2776, 2897, 3406
LguI GCTCTTC 1 cut(s) 438
LmnI GCTCC 9 cut(s) 226, 407, 1138, 1929, 2125, 2675, 2684, 2789, 3439
Lsp1109I GCAGC 5 cut(s) 74, 329, 2368, 3030, 3327
LweI GCATC 7 cut(s) 133, 583, 808, 1792, 2027, 2200, 3047
MabI ACCWGGT 1 cut(s) 1098
MaeI CTAG 4 cut(s) 26, 1475, 3300, 3312
MaeII ACGT 7 cut(s) 44, 565, 1455, 1620, 1737, 2728, 3593
MalI GATC 8 cut(s) 122, 1775, 2297, 2338, 2375, 2778, 2899, 3408
MbiI CCGCTC 1 cut(s) 15
MboI GATC 8 cut(s) 120, 1773, 2295, 2336, 2373, 2776, 2897, 3406
MflI RGATCY 1 cut(s) 1773
MhlI GDGCHC 7 cut(s) 113, 223, 263, 433, 1934, 2043, 2613
MlsI TGGCCA 2 cut(s) 2568, 2865
MluNI TGGCCA 2 cut(s) 2568, 2865
MlyI GAGTC 5 cut(s) 1273, 2801, 2930, 3041, 3421
MmeI TCCRAC 2 cut(s) 970, 1376
Mox20I TGGCCA 2 cut(s) 2568, 2865
MscI TGGCCA 2 cut(s) 2568, 2865
MslI CAYNNNNRTG 7 cut(s) 735, 926, 945, 1425, 1821, 2843, 3372
Msp20I TGGCCA 2 cut(s) 2568, 2865
MspA1I CMGCKG 1 cut(s) 2111
MspCI CTTAAG 1 cut(s) 2408
MspI CCGG 7 cut(s) 320, 370, 1663, 2787, 3056, 3295, 3437
Mva1269I GAATGC 2 cut(s) 2687, 3367
MvnI CGCG 2 cut(s) 1488, 2823
NciI CCSGG 3 cut(s) 1663, 1664, 2788
NcoI CCATGG 2 cut(s) 1833, 2838
NdeII GATC 8 cut(s) 120, 1773, 2295, 2336, 2373, 2776, 2897, 3406
NlaIV GGNNCC 9 cut(s) 286, 318, 448, 1350, 1383, 1775, 1858, 2469, 2785
NmuCI GTSAC 6 cut(s) 40, 45, 928, 949, 973, 2929
NspI RCATGY 1 cut(s) 950
NspV TTCGAA 1 cut(s) 181
PagI TCATGA 2 cut(s) 1054, 1615
PceI AGGCCT 1 cut(s) 137
PciI ACATGT 1 cut(s) 946
PciSI GCTCTTC 1 cut(s) 438
PctI GAATGC 2 cut(s) 2687, 3367
PfeI GAWTC 7 cut(s) 74, 700, 1667, 1813, 2903, 3214, 3329
PflMI CCANNNNNTGG 1 cut(s) 3536
PfoI TCCNGGA 3 cut(s) 547, 1882, 2710
PkrI GCNGC 8 cut(s) 64, 89, 92, 95, 344, 2383, 3020, 3317
PleI GAGTC 5 cut(s) 1273, 2800, 2930, 3041, 3421
PpsI GAGTC 5 cut(s) 1273, 2800, 2930, 3041, 3421
Ppu21I YACGTR 1 cut(s) 566
PpuMI RGGWCCY 1 cut(s) 446
PscI ACATGT 1 cut(s) 946
PshBI ATTAAT 1 cut(s) 363
Psp124BI GAGCTC 3 cut(s) 113, 1934, 2613
Psp5II RGGWCCY 1 cut(s) 446
PspN4I GGNNCC 9 cut(s) 286, 318, 448, 1350, 1383, 1775, 1858, 2469, 2785
PspPI GGNCC 8 cut(s) 284, 446, 1062, 1286, 2172, 2468, 2537, 3380
PspPPI RGGWCCY 1 cut(s) 446
PstNI CAGNNNCTG 3 cut(s) 782, 932, 2933
PsuI RGATCY 1 cut(s) 1773
PvuII CAGCTG 1 cut(s) 2111
RsaI GTAC 9 cut(s) 211, 775, 1485, 1890, 1991, 2104, 3000, 3071, 3596
RsaNI GTAC 9 cut(s) 210, 774, 1484, 1889, 1990, 2103, 2999, 3070, 3595
RseI CAYNNNNRTG 7 cut(s) 735, 926, 945, 1425, 1821, 2843, 3372
SacI GAGCTC 3 cut(s) 113, 1934, 2613
SapI GCTCTTC 1 cut(s) 438
SatI GCNGC 8 cut(s) 63, 88, 91, 94, 343, 2382, 3019, 3316
Sau3AI GATC 8 cut(s) 120, 1773, 2295, 2336, 2373, 2776, 2897, 3406
Sau96I GGNCC 8 cut(s) 284, 446, 1062, 1286, 2172, 2468, 2537, 3380
ScaI AGTACT 1 cut(s) 211
SchI GAGTC 5 cut(s) 1273, 2801, 2930, 3041, 3421
SduI GDGCHC 7 cut(s) 113, 223, 263, 433, 1934, 2043, 2613
SexAI ACCWGGT 1 cut(s) 1098
SfaNI GCATC 7 cut(s) 133, 583, 808, 1792, 2027, 2200, 3047
SfcI CTRYAG 3 cut(s) 1494, 2577, 2653
SfiI GGCCNNNNNGGCC 1 cut(s) 134
SfuI TTCGAA 1 cut(s) 181
SinI GGWCC 5 cut(s) 284, 446, 1062, 1286, 2537
SmaI CCCGGG 1 cut(s) 1664
SmiMI CAYNNNNRTG 7 cut(s) 735, 926, 945, 1425, 1821, 2843, 3372
SmlI CTYRAG 1 cut(s) 2408
SmoI CTYRAG 1 cut(s) 2408
SpeI ACTAGT 2 cut(s) 25, 1474
SseBI AGGCCT 1 cut(s) 137
SspI AATATT 2 cut(s) 541, 708
SspMI CTAG 4 cut(s) 26, 1475, 3300, 3312
SstI GAGCTC 3 cut(s) 113, 1934, 2613
StuI AGGCCT 1 cut(s) 137
StyI CCWWGG 6 cut(s) 289, 763, 1833, 2838, 3270, 3491
TaiI ACGT 7 cut(s) 47, 568, 1458, 1623, 1740, 2731, 3596
TaqI TCGA 8 cut(s) 102, 181, 465, 1344, 1709, 2314, 3141, 3348
TatI WGTACW 4 cut(s) 209, 1888, 2102, 2998
TauI GCSGC 3 cut(s) 65, 93, 96
TfiI GAWTC 7 cut(s) 74, 700, 1667, 1813, 2903, 3214, 3329
TseFI GTSAC 6 cut(s) 40, 45, 928, 949, 973, 2929
TseI GCWGC 5 cut(s) 87, 342, 2381, 3018, 3315
Tsp45I GTSAC 6 cut(s) 40, 45, 928, 949, 973, 2929
TspGWI ACGGA 4 cut(s) 297, 1023, 1766, 3211
TspMI CCCGGG 1 cut(s) 1662
Van91I CCANNNNNTGG 1 cut(s) 3536
Vha464I CTTAAG 1 cut(s) 2408
VneI GTGCAC 1 cut(s) 2039
VpaK11BI GGWCC 5 cut(s) 284, 446, 1062, 1286, 2537
VspI ATTAAT 1 cut(s) 363
XapI RAATTY 8 cut(s) 830, 1333, 1460, 1604, 1751, 2056, 2077, 3323
XceI RCATGY 1 cut(s) 950
XmaI CCCGGG 1 cut(s) 1662
XmiI GTMKAC 3 cut(s) 249, 1402, 2067
XspI CTAG 4 cut(s) 26, 1475, 3300, 3312
ZraI GACGTC 2 cut(s) 45, 1621
ZrmI AGTACT 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.