MD17G1277500.v1.1

Nodal modulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0011945
Physical Location & Seq
Forward (+)
33754167 .. 33767301
13135 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1277500.v1.1.491

Sequence Viewer

Length: 3600 bp
ATGGGCATCAAAGACCACGCCTCAGCCTTCTTCTTCTTCCTCGCAATCTCTTCCCTCTCTACCACTTTCGCCGATTCAATCCACGGCTGCGGCGGCTTCGTCGAGGCTAGTTCTTCTCTGATCAAGACCAGGAAGGCCACCGGTGTTAAATTGGATTATTCTCACATCACGGTTGAGCTTCGGACAGTTGATGGGTTGTTGAAGGATAGCACTCAATGTGCTCCCAATGGTTACTACTTCATTCCCGTATATGACAAGGGTTCGTTTGTAATTAAAATCAATGGCCCAGAGGGATGGTCATGGAACCCAGACAAGGTTCCTGTTGTTGTTGACGACTCTGGCTGCAATGGCAGTGAAGATATTAACTTTCGGTTTACAGGGTTCTCCTTGTCTGGTAGAGTAGTGGGAGCCGTTGGCGGATGGAGCTGCTCCGTTCAAAATGGAGGTCCTCCAAATATTGAAGTTGAACTTTTGTCTGATACTGGTGATGTTGTTTCCTCTGTTATAACATCAGCAGGGGGTGTTTACGTGTTCAAAAATATTATTCCAGGAAAATATGAATTACGTGCTTCACACCCTGACTTGAAAGTTGAAATAAGTGGTTCAACAAAGGTGAATTTGGGTTTCGGAAATGACGTAGTGGATGACATTTTCTTTGTCCCTGGGTATGATGTTCGTGGATTTGTTGTTTCTCAGGGAAATCCAATACTGGGAGTTCACGTTTATTTATACTCAGATGACGTCTTAGAGGTAGATTGTCCCCAAGGTTCTGGAACTGCCTCTGGAATGAGGAAGGCACTCTGCCATGCTGTATCCGATGCTCATGGGATGTTCATTTTTAAATCAGTACCTTGTGGAACATATGAGCTCATACCTTACTACAAGGGTGAAAACACAGTGTTTGATGTTTCACCTCCTGTCATGTCAGTAACTGTTGAGCATCAACACGTGACAGTTCCTCAGAAGTTCCAGGTCACTGGATTTTCTGTTGGGGGCCGAGTTGTGGATGGAAATGATGAAGGCGTTGAAGGTGTTAAAATTATAGTTGATGGCCATGAAAGGTCCATCACTGACAAACAAGGGTATTACAAACTTGACCAGGTTACTTCCAATCGCTATGCAATTGAAGCCACAAAGGAGCATTACAAGTTCAGCAGTTTGAACGATTATTTGGTATTACCGAATATGGCTTCAATTATGGATATTAAAGCAGTTTCCTATGATGTCTGTGGTGTGGTTCATATGGTTACTGCTGGTTACAAGGCAAAGGTAGCTTTGACTCATGGCCCTGAAAATGTCAAACCGCAAGTGAAACAGACTGATGGAAGTGGAATTTTCTGCTTTGAGGTTACACCTGGTGAATATCGTTTATCTGCTTTAGCAGCTACGCCTGAGAGTGCTTCTGGTCTAATGTTTTTGCCGTCTTATATTGATGTTGCTGTCAAAAATCCATTATTGAACGTCAAGTTTTCTCAGGCATTGGTCAATGTACGTGGAACTGTAACCTGCAAGGAGAAGTGTGGTGCATCTGTTTCTGTTACCCTGGTTGGTTTAGCTGGTAAACGTAATGAAGAGAGGAGGACGGTTAGTTTGACCAATGAGAGTAGTGAATTTCACTTCGAAAGTGTTATCCCAGGGAAATACAGATTTGAGGTCAAACATCATTCAGAAGAACCCACAGCTGTTGAAGATAATTGGTGCTGGGAGAAAAGTTCCATTGATGTGGATGTTGGTGTGGATGATGTAAAAGGAATTGAATTTGTTCAAAAAGGCTACTGGGTCAATGTCATTTCCACCCACAATGTAGATGCTTATATGACTCAGCCAGATGGTTCTTCCATTAATTTGAAAATTAAGAAAGGTTCCCAGAATATATGTATAGAATACCCTGGAGTGCATGAACTTTATTTTGTCAACTCGTGCATTTTCTTCGGGAGCTCATCAGTTGAGATTGATACTTCAAACCCATTGCCTATTTATCTGAAAGGAGAAAAGCATCTTGTTAAAGGACAAATCAATGTTTCAACAAGCTCATTTGAGGGTGTCAGCGAGGTACCTGAGAATTTCATTGTGGATATTCTGAATGCCGGGGGTAGTATTATAGATGAGACCACAGCTTGGCTGTCTTCCAGGGGAAATGATCAAAGTGCCGTAGTGTATGAATATTCTGCATGGGCCAATCGTGGAGAAAGACTTACCTTTGTTCCTCGGGATCCAAGAACTGATGAGACGAGGAAGATCTTGTTTTATCCTAGACAACATCATGTCTTAATTACAAATGATGGCTGTCAAGCTTCAATCCCTCCATTCTCTGGTAGATTGGGTTTATATATTAAGGGATCAGTCTCTCCCCCTCTTTCTGAAGTTCATATTAAGATTCTTGCCTCTGGGGATAGCCAGATTGCTCAACTTAAGGATGGTGAACTAGTACTTGAAACTACTACGGGAATGGATGGCTCTTTTGTTGGGGGACCTTTATATGATGAAATAACATACCGTGTCGAGGCTTCAAAGCCTGGATACCATTTAAAACAAGTTGGGCCTCATTCCTTTTCTTGTCAGAAGCTTGGCCAAATTTCTGTAAATATTCATTCTAAAGATGATGCTAAAGAGCCTATTCCTTCAGTGTTATTATCTTTGAGTGGTGATGATGGCTACAGAAACAACTCAATATCTGGAGCTGGTGGAGCATTCCTATTCAACAACTTATTTCCAGGGACATTCTATCTCCGTCCTCTGCTAAAGGAGTTTGCCTTTTCTCCTCCGGCACTGGCAATAGATCTTGGTTCTGGGGAGTCTAAAGAAGCTATTTTCCTGGCTACTCGAGTGGCTTACAGTGCTATGGGTGTCGTTACTCTATTGTCTGGCCAACCAAAAGAAGGTGTGTTGGTTGAGGCCAGATCAGAATCAAAAGGCTATTATGAGGAAACAGTGACTGATTCATCCGGAAGTTATCGTTTGAGAGGACTTCTTCCTGACGCAATCTATGTAATCAAAGTAGTGAAAAGGGATGGTTTGGGTAGCGCTAAAATTGAGCGCGCATCACCAGAATATGTTCCTATCACGGTTGGGCACGAGGATATCAAAGGATTAGATTTTCTGGTTTTTGAACAACCAGACACAACTATTTTAAGTTGCCATGTTGAAGGAAAGAGAATAAAGGAACTGCATCCACATCTACTAGTGGAAATCAAATCTAGTGATCTATCTATAACTGAGTCCGTCTTCCCTCTTCCGCTTTCCAACTTTTTCCAGGTGAAGGACTTACCCAAGGGTAAGCACCTTCTGCAGCTTCGTTATAGCCTACCGTCAAGCAGTCATAAGTTTAAATCCGAGGTTATCGAGGTTGATTTAGAGCAGAATACACGCATACATGTTGGCCCACTCAGATATGCATTTGAAGAGAATCAGCAGAAACAGGAGTTGACTCCGGCACCAGTTTTTCCTCTTATAGTCGGCGTCTCAGTGATTGCTCTGTTTGCCACTATTCCAAGGTTGAAGGACTTATACCAATCCACGGTGGGAATACCAACGCCTGGATTCACGACAACTGCAAAGAAGGAAGTACGAAAGCCTGTTTTGAGAAAGAAGACTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CarboxypepD_reg

Protein Analysis

1200

Amino Acids

130.89

Weight (kDa)

5.78

Isoelectric Point (pI)

30.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NOMO1-like_1st PF22898 47 - 123 2e-32 BOS complex subunit NOMO1-like, first beta sandwich domain
NOMO1-like_2nd PF22904 125 - 217 1.2e-15 BOS complex subunit NOMO1-like, second beta sandwich domain
NOMO_3rd PF23193 225 - 326 2e-09 NOMO third transthyretin-like domain
NOMO1-like_2nd PF22904 326 - 386 4.8e-07 BOS complex subunit NOMO1-like, second beta sandwich domain
CarboxypepD_reg PF13620 329 - 388 2.7e-06 Carboxypeptidase regulatory-like domain
NOMO_5th PF23194 408 - 490 6.7e-10 NOMO fifth transthyretin-like domain
NOMO_6th PF23196 495 - 572 8.2e-07 NOMO sixth transthyretin-like domain
Ig_NOMO PF23141 593 - 665 9.2e-26 NOMO Ig-like domain
NOMO_8th PF23660 667 - 773 3.1e-39 NOMO eighth prealbumin-like domain
NOMO1-like_9th PF22902 775 - 847 1.1e-12 BOS complex subunit NOMO1-like, beta sandwich domain
CarboxypepD_reg PF13620 949 - 1008 1.3e-06 Carboxypeptidase regulatory-like domain
DUF7152 PF23662 1041 - 1138 7e-44 Domain of unknown function (DUF7152)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011945)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G62360 AT3G62360
fragaria_vesca FvH4_1g27060
malus_domestica MD09G1284300.v1.1 MD17G1277500.v1.1
prunus_persica Prupe.3G010500_v2.0.a1 Prupe.3G010500_v2.0.a1 Prupe.3G010500_v2.0.a1
pyrus_communis pycom17g27510
rosa_chinensis RchiOBHm_Chr3g0487381
rosa_laevigata RLG00000023009
rosa_multiflora Rmu_sc0001955.1_g000004
rosa_roxburghii Rroxscaffold_6G00394840
rosa_rugosa Rorug03G0233600
rosa_samantha Rh3AG281600 Rh3BG317200 Rh3CG315300 Rh3DG313200
rosa_wichuraiana Rw3G024930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 506
AatII GACGTC 1 cut(s) 744
Acc36I ACCTGC 1 cut(s) 1514
Acc65I GGTACC 1 cut(s) 2053
AccB1I GGYRCC 2 cut(s) 2053, 3433
AccB7I CCANNNNNTGG 3 cut(s) 2118, 2312, 3536
AccII CGCG 1 cut(s) 3039
AccIII TCCGGA 1 cut(s) 2945
AciI CCGC 5 cut(s) 90, 93, 417, 1304, 3236
AclWI GGATC 3 cut(s) 2207, 2220, 2347
AcoI YGGCCR 3 cut(s) 1051, 2569, 2866
AcsI RAATTY 6 cut(s) 616, 1332, 1610, 1757, 2062, 2574
AcuI CTGAAG 2 cut(s) 2382, 2607
AcvI CACGTG 1 cut(s) 949
AcyI GRCGYC 2 cut(s) 741, 3459
AdeI CACNNNGTG 1 cut(s) 1358
AfaI GTAC 5 cut(s) 849, 1491, 2055, 2430, 3567
AfeI AGCGCT 1 cut(s) 3025
AflII CTTAAG 1 cut(s) 2411
AflIII ACRYGT 3 cut(s) 528, 946, 3373
AgeI ACCGGT 1 cut(s) 140
AhlI ACTAGT 2 cut(s) 2425, 3181
AjuI GAANNNNNNNTTGG 2 cut(s) 1154, 1186
Alw21I GWGCWC 3 cut(s) 223, 870, 1940
Alw26I GTCTC 4 cut(s) 2102, 2222, 2350, 3466
AlwI GGATC 3 cut(s) 2207, 2220, 2347
AlwNI CAGNNNCTG 2 cut(s) 932, 2936
Ama87I CYCGRG 2 cut(s) 2208, 2823
Aor13HI TCCGGA 1 cut(s) 2945
Aor51HI AGCGCT 1 cut(s) 3025
ApeKI GCWGC 5 cut(s) 87, 342, 426, 1382, 3289
ApoI RAATTY 6 cut(s) 616, 1332, 1610, 1757, 2062, 2574
ArsI GACNNNNNNTTYG 6 cut(s) 430, 462, 638, 670, 1573, 1605
AseI ATTAAT 1 cut(s) 1842
AsiGI ACCGGT 1 cut(s) 140
Asp700I GAANNNNTTC 2 cut(s) 1761, 3054
Asp718I GGTACC 1 cut(s) 2053
AspLEI GCGC 3 cut(s) 3026, 3039, 3041
AspS9I GGNCC 9 cut(s) 284, 446, 994, 1062, 1286, 2175, 2471, 2540, 3380
AsuC2I CCSGG 1 cut(s) 2089
AsuHPI GGTGA 9 cut(s) 497, 625, 899, 903, 1370, 2432, 2657, 3036, 3268
AsuII TTCGAA 1 cut(s) 1620
AvaI CYCGRG 2 cut(s) 2208, 2823
AvaII GGWCC 3 cut(s) 446, 1062, 2471
BaeGI GKGCMC 1 cut(s) 3075
BalI TGGCCA 3 cut(s) 1053, 2571, 2868
BamHI GGATCC 1 cut(s) 2212
BanI GGYRCC 2 cut(s) 2053, 3433
BanII GRGCYC 2 cut(s) 870, 1940
BarI GAAGNNNNNNTAC 2 cut(s) 3491, 3523
BauI CACGAG 2 cut(s) 1918, 3074
BbrPI CACGTG 1 cut(s) 949
BbsI GAAGAC 3 cut(s) 2118, 3217, 3596
Bbv12I GWGCWC 3 cut(s) 223, 870, 1940
BbvCI CCTCAGC 1 cut(s) 22
BbvI GCAGC 5 cut(s) 74, 329, 413, 1394, 3301
BceAI ACGGC 4 cut(s) 100, 395, 1405, 2135
BcgI CGANNNNNNTGC 2 cut(s) 1912, 1946
BciVI GTATCC 2 cut(s) 823, 2513
BclI TGATCA 2 cut(s) 120, 2140
BcnI CCSGG 1 cut(s) 2089
BcoDI GTCTC 4 cut(s) 2102, 2222, 2350, 3466
BcuI ACTAGT 2 cut(s) 2425, 3181
BfaI CTAG 5 cut(s) 108, 2253, 2426, 3182, 3198
BfmI CTRYAG 2 cut(s) 2656, 3287
BfoI RGCGCY 1 cut(s) 3027
BfrI CTTAAG 1 cut(s) 2411
BfuAI ACCTGC 1 cut(s) 1514
BfuI GTATCC 2 cut(s) 823, 2513
BglII AGATCT 2 cut(s) 2238, 2779
BisI GCNGC 7 cut(s) 88, 91, 94, 343, 427, 1383, 3290
BlsI GCNGC 7 cut(s) 89, 92, 95, 344, 428, 1384, 3291
BmcAI AGTACT 1 cut(s) 2430
Bme18I GGWCC 3 cut(s) 446, 1062, 2471
BmeT110I CYCGRG 2 cut(s) 2208, 2823
BmgT120I GGNCC 9 cut(s) 284, 446, 994, 1062, 1286, 2175, 2471, 2540, 3380
BmiI GGNNCC 9 cut(s) 305, 318, 409, 995, 1864, 2055, 2214, 2472, 3435
BmrI ACTGGG 2 cut(s) 719, 1786
BmsI GCATC 9 cut(s) 15, 808, 949, 1535, 1798, 2005, 2593, 3050, 3178
BmuI ACTGGG 2 cut(s) 719, 1786
BpiI GAAGAC 3 cut(s) 2118, 3217, 3596
BpmI CTGGAG 2 cut(s) 1911, 2697
Bpu10I CCTNAGC 1 cut(s) 22
Bpu14I TTCGAA 1 cut(s) 1620
BpuMI CCSGG 1 cut(s) 2089
BsaAI YACGTR 4 cut(s) 529, 566, 949, 1493
BsaBI GATNNNNATC 1 cut(s) 2905
BsaHI GRCGYC 2 cut(s) 741, 3459
BsaI GGTCTC 1 cut(s) 2102
BsaWI WCCGGW 2 cut(s) 140, 2945
BsaXI ACNNNNNCTCC 2 cut(s) 705, 735
Bse118I RCCGGY 1 cut(s) 140
Bse1I ACTGG 6 cut(s) 487, 714, 982, 1781, 2775, 3437
Bse3DI GCAATG 2 cut(s) 352, 1967
Bse8I GATNNNNATC 1 cut(s) 2905
BseAI TCCGGA 1 cut(s) 2945
BseJI GATNNNNATC 1 cut(s) 2905
BseMI GCAATG 2 cut(s) 352, 1967
BseNI ACTGG 6 cut(s) 487, 714, 982, 1781, 2775, 3437
BsePI GCGCGC 1 cut(s) 3037
BseRI GAGGAG 2 cut(s) 1591, 2751
BseSI GKGCMC 1 cut(s) 3075
BseXI GCAGC 5 cut(s) 74, 329, 413, 1394, 3301
BseYI CCCAGC 1 cut(s) 1701
Bsh1236I CGCG 1 cut(s) 3039
BshNI GGYRCC 2 cut(s) 2053, 3433
BshTI ACCGGT 1 cut(s) 140
BsiHKAI GWGCWC 3 cut(s) 223, 870, 1940
BsiHKCI CYCGRG 2 cut(s) 2208, 2823
BsiSI CCGG 5 cut(s) 141, 2088, 2765, 2946, 3431
BslFI GGGAC 4 cut(s) 644, 744, 2484, 2731
BsmAI GTCTC 4 cut(s) 2102, 2222, 2350, 3466
BsmBI CGTCTC 2 cut(s) 2222, 3466
BsmFI GGGAC 4 cut(s) 644, 744, 2484, 2731
BsmI GAATGC 2 cut(s) 2089, 2690
Bso31I GGTCTC 1 cut(s) 2102
BsoBI CYCGRG 2 cut(s) 2208, 2823
Bsp119I TTCGAA 1 cut(s) 1620
Bsp1286I GDGCHC 4 cut(s) 223, 870, 1940, 3075
Bsp13I TCCGGA 1 cut(s) 2945
Bsp143I GATC 8 cut(s) 120, 2140, 2212, 2238, 2339, 2779, 2900, 3202
BspACI CCGC 5 cut(s) 90, 93, 417, 1304, 3236
BspEI TCCGGA 1 cut(s) 2945
BspFNI CGCG 1 cut(s) 3039
BspLI GGNNCC 9 cut(s) 305, 318, 409, 995, 1864, 2055, 2214, 2472, 3435
BspMAI CTGCAG 1 cut(s) 3291
BspMI ACCTGC 1 cut(s) 1514
BspPI GGATC 3 cut(s) 2207, 2220, 2347
BspT104I TTCGAA 1 cut(s) 1620
BspT107I GGYRCC 2 cut(s) 2053, 3433
BspTI CTTAAG 1 cut(s) 2411
BspTNI GGTCTC 1 cut(s) 2102
BsrDI GCAATG 2 cut(s) 352, 1967
BsrFI RCCGGY 1 cut(s) 140
BsrI ACTGG 6 cut(s) 487, 714, 982, 1781, 2775, 3437
BssAI RCCGGY 1 cut(s) 140
BssHII GCGCGC 1 cut(s) 3037
BssMI GATC 8 cut(s) 120, 2140, 2212, 2238, 2339, 2779, 2900, 3202
BssNI GRCGYC 2 cut(s) 741, 3459
BssSI CACGAG 2 cut(s) 1918, 3074
BssT1I CCWWGG 3 cut(s) 763, 3270, 3491
Bst2BI CACGAG 2 cut(s) 1918, 3074
Bst6I CTCTTC 4 cut(s) 55, 1566, 3237, 3396
BstACI GRCGYC 2 cut(s) 741, 3459
BstAFI CTTAAG 1 cut(s) 2411
BstAPI GCANNNNNTGC 1 cut(s) 3286
BstBAI YACGTR 4 cut(s) 529, 566, 949, 1493
BstBI TTCGAA 1 cut(s) 1620
BstC8I GCNNGC 1 cut(s) 3039
BstDSI CCRYGG 2 cut(s) 82, 3516
BstFNI CGCG 1 cut(s) 3039
BstH2I RGCGCY 1 cut(s) 3027
BstHHI GCGC 3 cut(s) 3026, 3039, 3041
BstKTI GATC 8 cut(s) 123, 2143, 2215, 2241, 2342, 2782, 2903, 3205
BstMAI GTCTC 4 cut(s) 2102, 2222, 2350, 3466
BstMBI GATC 8 cut(s) 120, 2140, 2212, 2238, 2339, 2779, 2900, 3202
BstNSI RCATGY 1 cut(s) 3377
BstSFI CTRYAG 2 cut(s) 2656, 3287
BstSLI GKGCMC 1 cut(s) 3075
BstUI CGCG 1 cut(s) 3039
BstV1I GCAGC 5 cut(s) 74, 329, 413, 1394, 3301
BstV2I GAAGAC 3 cut(s) 2118, 3217, 3596
BstX2I RGATCY 3 cut(s) 2212, 2238, 2779
BstXI CCANNNNNNTGG 4 cut(s) 294, 770, 977, 1723
BstYI RGATCY 3 cut(s) 2212, 2238, 2779
BsuI GTATCC 2 cut(s) 823, 2513
BtgI CCRYGG 2 cut(s) 82, 3516
BtsI GCAGTG 1 cut(s) 358
BtsIMutI CAGTG 9 cut(s) 358, 903, 975, 1068, 2631, 2768, 2842, 2937, 3471
BveI ACCTGC 1 cut(s) 1514
Cac8I GCNNGC 1 cut(s) 3039
CaiI CAGNNNCTG 2 cut(s) 932, 2936
CfoI GCGC 3 cut(s) 3026, 3039, 3041
Cfr10I RCCGGY 1 cut(s) 140
Cfr13I GGNCC 9 cut(s) 284, 446, 994, 1062, 1286, 2175, 2471, 2540, 3380
CseI GACGC 2 cut(s) 2987, 3448
CsiI ACCWGGT 2 cut(s) 1098, 1354
Csp6I GTAC 5 cut(s) 848, 1490, 2054, 2429, 3566
CspAI ACCGGT 1 cut(s) 140
CspCI CAANNNNNGTGG 4 cut(s) 1474, 1509, 1666, 1701
CviQI GTAC 5 cut(s) 848, 1490, 2054, 2429, 3566
DpnI GATC 8 cut(s) 122, 2142, 2214, 2240, 2341, 2781, 2902, 3204
DpnII GATC 8 cut(s) 120, 2140, 2212, 2238, 2339, 2779, 2900, 3202
DraI TTTAAA 3 cut(s) 841, 2529, 3328
DraIII CACNNNGTG 1 cut(s) 1358
EaeI YGGCCR 3 cut(s) 1051, 2569, 2866
Eam1104I CTCTTC 4 cut(s) 55, 1566, 3237, 3396
EarI CTCTTC 4 cut(s) 55, 1566, 3237, 3396
EciI GGCGGA 1 cut(s) 432
Ecl136II GAGCTC 2 cut(s) 868, 1938
Eco130I CCWWGG 3 cut(s) 763, 3270, 3491
Eco24I GRGCYC 2 cut(s) 870, 1940
Eco31I GGTCTC 1 cut(s) 2102
Eco32I GATATC 1 cut(s) 3082
Eco47I GGWCC 3 cut(s) 446, 1062, 2471
Eco47III AGCGCT 1 cut(s) 3025
Eco53kI GAGCTC 2 cut(s) 868, 1938
Eco57I CTGAAG 2 cut(s) 2382, 2607
Eco72I CACGTG 1 cut(s) 949
Eco88I CYCGRG 2 cut(s) 2208, 2823
EcoICRI GAGCTC 2 cut(s) 868, 1938
EcoO109I RGGNCCY 2 cut(s) 446, 2471
EcoRV GATATC 1 cut(s) 3082
EcoT14I CCWWGG 3 cut(s) 763, 3270, 3491
EcoT22I ATGCAT 1 cut(s) 3397
EcoT38I GRGCYC 2 cut(s) 870, 1940
ErhI CCWWGG 3 cut(s) 763, 3270, 3491
Esp3I CGTCTC 2 cut(s) 2222, 3466
FalI AAGNNNNNCTT 2 cut(s) 453, 485
FaqI GGGAC 4 cut(s) 644, 744, 2484, 2731
FauNDI CATATG 2 cut(s) 862, 1242
FbaI TGATCA 2 cut(s) 120, 2140
Fnu4HI GCNGC 7 cut(s) 88, 91, 94, 343, 427, 1383, 3290
FriOI GRGCYC 2 cut(s) 870, 1940
Fsp4HI GCNGC 7 cut(s) 88, 91, 94, 343, 427, 1383, 3290
FspBI CTAG 5 cut(s) 108, 2253, 2426, 3182, 3198
GlaI GCGC 3 cut(s) 3025, 3038, 3040
GluI GCNGC 7 cut(s) 88, 91, 94, 343, 427, 1383, 3290
GsaI CCCAGC 1 cut(s) 1705
GsuI CTGGAG 2 cut(s) 1911, 2697
HaeII RGCGCY 1 cut(s) 3027
HapII CCGG 5 cut(s) 141, 2088, 2765, 2946, 3431
HgaI GACGC 2 cut(s) 2987, 3448
HhaI GCGC 3 cut(s) 3026, 3039, 3041
Hin1I GRCGYC 2 cut(s) 741, 3459
Hin6I GCGC 3 cut(s) 3024, 3037, 3039
HinP1I GCGC 3 cut(s) 3024, 3037, 3039
HincII GTYRAC 3 cut(s) 331, 1915, 3426
HindII GTYRAC 3 cut(s) 331, 1915, 3426
HindIII AAGCTT 2 cut(s) 2292, 2564
HpaII CCGG 5 cut(s) 141, 2088, 2765, 2946, 3431
HphI GGTGA 9 cut(s) 497, 625, 899, 903, 1370, 2432, 2657, 3036, 3268
Hpy166II GTNNAC 8 cut(s) 331, 375, 526, 718, 1562, 1915, 2423, 3426
Hpy188III TCNNGA 9 cut(s) 124, 771, 783, 1933, 2210, 2676, 2946, 2975, 3544
Hpy8I GTNNAC 8 cut(s) 331, 375, 526, 718, 1562, 1915, 2423, 3426
Hpy99I CGWCG 1 cut(s) 104
HpyCH4IV ACGT 9 cut(s) 528, 565, 636, 720, 741, 948, 1461, 1492, 1564
HpySE526I ACGT 9 cut(s) 528, 565, 636, 720, 741, 948, 1461, 1492, 1564
Hsp92I GRCGYC 2 cut(s) 741, 3459
HspAI GCGC 3 cut(s) 3024, 3037, 3039
Kpn2I TCCGGA 1 cut(s) 2945
KpnI GGTACC 1 cut(s) 2057
Ksp22I TGATCA 2 cut(s) 120, 2140
Kzo9I GATC 8 cut(s) 120, 2140, 2212, 2238, 2339, 2779, 2900, 3202
LmnI GCTCC 8 cut(s) 226, 407, 423, 434, 1138, 1935, 2678, 2687
Lsp1109I GCAGC 5 cut(s) 74, 329, 413, 1394, 3301
LweI GCATC 9 cut(s) 15, 808, 949, 1535, 1798, 2005, 2593, 3050, 3178
MabI ACCWGGT 2 cut(s) 1098, 1354
MaeI CTAG 5 cut(s) 108, 2253, 2426, 3182, 3198
MaeII ACGT 9 cut(s) 528, 565, 636, 720, 741, 948, 1461, 1492, 1564
MalI GATC 8 cut(s) 122, 2142, 2214, 2240, 2341, 2781, 2902, 3204
MboI GATC 8 cut(s) 120, 2140, 2212, 2238, 2339, 2779, 2900, 3202
MfeI CAATTG 1 cut(s) 1122
MflI RGATCY 3 cut(s) 2212, 2238, 2779
MhlI GDGCHC 4 cut(s) 223, 870, 1940, 3075
MlsI TGGCCA 3 cut(s) 1053, 2571, 2868
MluNI TGGCCA 3 cut(s) 1053, 2571, 2868
MlyI GAGTC 6 cut(s) 329, 1273, 1813, 2804, 3227, 3421
MmeI TCCRAC 1 cut(s) 3267
Mox20I TGGCCA 3 cut(s) 1053, 2571, 2868
Mph1103I ATGCAT 1 cut(s) 3397
MroI TCCGGA 1 cut(s) 2945
MroXI GAANNNNTTC 2 cut(s) 1761, 3054
MscI TGGCCA 3 cut(s) 1053, 2571, 2868
MslI CAYNNNNRTG 1 cut(s) 1721
Msp20I TGGCCA 3 cut(s) 1053, 2571, 2868
MspA1I CMGCKG 1 cut(s) 1682
MspCI CTTAAG 1 cut(s) 2411
MspI CCGG 5 cut(s) 141, 2088, 2765, 2946, 3431
MunI CAATTG 1 cut(s) 1122
Mva1269I GAATGC 2 cut(s) 2089, 2690
MvnI CGCG 1 cut(s) 3039
NciI CCSGG 1 cut(s) 2089
NdeI CATATG 2 cut(s) 862, 1242
NdeII GATC 8 cut(s) 120, 2140, 2212, 2238, 2339, 2779, 2900, 3202
NlaIV GGNNCC 9 cut(s) 305, 318, 409, 995, 1864, 2055, 2214, 2472, 3435
NmeAIII GCCGAG 1 cut(s) 1022
NmuCI GTSAC 3 cut(s) 949, 973, 2932
NsiI ATGCAT 1 cut(s) 3397
NspI RCATGY 1 cut(s) 3377
NspV TTCGAA 1 cut(s) 1620
PaeR7I CTCGAG 1 cut(s) 2823
PasI CCCWGGG 2 cut(s) 662, 1634
PauI GCGCGC 1 cut(s) 3037
PciI ACATGT 1 cut(s) 3373
PcsI WCGNNNNNNNCGW 1 cut(s) 633
PctI GAATGC 2 cut(s) 2089, 2690
PdmI GAANNNNTTC 2 cut(s) 1761, 3054
PfeI GAWTC 6 cut(s) 74, 2377, 2906, 2939, 3406, 3540
PflMI CCANNNNNTGG 3 cut(s) 2118, 2312, 3536
PfoI TCCNGGA 1 cut(s) 547
PinAI ACCGGT 1 cut(s) 140
PkrI GCNGC 7 cut(s) 89, 92, 95, 344, 428, 1384, 3291
PleI GAGTC 6 cut(s) 329, 1273, 1813, 2803, 3226, 3421
PmaCI CACGTG 1 cut(s) 949
PmlI CACGTG 1 cut(s) 949
PpsI GAGTC 6 cut(s) 329, 1273, 1813, 2803, 3226, 3421
Ppu21I YACGTR 4 cut(s) 529, 566, 949, 1493
PpuMI RGGWCCY 2 cut(s) 446, 2471
PscI ACATGT 1 cut(s) 3373
PshBI ATTAAT 1 cut(s) 1842
PsiI TTATAA 1 cut(s) 506
Psp124BI GAGCTC 2 cut(s) 870, 1940
Psp5II RGGWCCY 2 cut(s) 446, 2471
PspCI CACGTG 1 cut(s) 949
PspFI CCCAGC 1 cut(s) 1701
PspN4I GGNNCC 9 cut(s) 305, 318, 409, 995, 1864, 2055, 2214, 2472, 3435
PspPI GGNCC 9 cut(s) 284, 446, 994, 1062, 1286, 2175, 2471, 2540, 3380
PspPPI RGGWCCY 2 cut(s) 446, 2471
PspXI VCTCGAGB 1 cut(s) 2823
PstI CTGCAG 1 cut(s) 3291
PstNI CAGNNNCTG 2 cut(s) 932, 2936
PsuI RGATCY 3 cut(s) 2212, 2238, 2779
PteI GCGCGC 1 cut(s) 3037
PvuII CAGCTG 1 cut(s) 1682
RsaI GTAC 5 cut(s) 849, 1491, 2055, 2430, 3567
RsaNI GTAC 5 cut(s) 848, 1490, 2054, 2429, 3566
RseI CAYNNNNRTG 1 cut(s) 1721
SacI GAGCTC 2 cut(s) 870, 1940
SatI GCNGC 7 cut(s) 88, 91, 94, 343, 427, 1383, 3290
Sau3AI GATC 8 cut(s) 120, 2140, 2212, 2238, 2339, 2779, 2900, 3202
Sau96I GGNCC 9 cut(s) 284, 446, 994, 1062, 1286, 2175, 2471, 2540, 3380
ScaI AGTACT 1 cut(s) 2430
SchI GAGTC 6 cut(s) 329, 1273, 1813, 2804, 3227, 3421
SduI GDGCHC 4 cut(s) 223, 870, 1940, 3075
SexAI ACCWGGT 2 cut(s) 1098, 1354
SfaNI GCATC 9 cut(s) 15, 808, 949, 1535, 1798, 2005, 2593, 3050, 3178
SfcI CTRYAG 2 cut(s) 2656, 3287
Sfr274I CTCGAG 1 cut(s) 2823
SfuI TTCGAA 1 cut(s) 1620
SgrAI CRCCGGYG 1 cut(s) 140
SinI GGWCC 3 cut(s) 446, 1062, 2471
SlaI CTCGAG 1 cut(s) 2823
SmiMI CAYNNNNRTG 1 cut(s) 1721
SmlI CTYRAG 2 cut(s) 2411, 2823
SmoI CTYRAG 2 cut(s) 2411, 2823
SpeI ACTAGT 2 cut(s) 2425, 3181
SsiI CCGC 5 cut(s) 90, 93, 417, 1304, 3236
SspI AATATT 4 cut(s) 457, 541, 2165, 2587
SspMI CTAG 5 cut(s) 108, 2253, 2426, 3182, 3198
SstI GAGCTC 2 cut(s) 870, 1940
StyI CCWWGG 3 cut(s) 763, 3270, 3491
TaiI ACGT 9 cut(s) 531, 568, 639, 723, 744, 951, 1464, 1495, 1567
TaqI TCGA 5 cut(s) 102, 1620, 2502, 2824, 3342
TatI WGTACW 1 cut(s) 2428
TauI GCSGC 2 cut(s) 93, 96
TfiI GAWTC 6 cut(s) 74, 2377, 2906, 2939, 3406, 3540
TscAI CASTG 9 cut(s) 358, 903, 982, 1075, 2631, 2775, 2842, 2937, 3471
TseFI GTSAC 3 cut(s) 949, 973, 2932
TseI GCWGC 5 cut(s) 87, 342, 426, 1382, 3289
Tsp45I GTSAC 3 cut(s) 949, 973, 2932
TspGWI ACGGA 3 cut(s) 421, 2720, 3211
TspRI CASTG 9 cut(s) 358, 903, 982, 1075, 2631, 2775, 2842, 2937, 3471
Van91I CCANNNNNTGG 3 cut(s) 2118, 2312, 3536
Vha464I CTTAAG 1 cut(s) 2411
VpaK11BI GGWCC 3 cut(s) 446, 1062, 2471
VspI ATTAAT 1 cut(s) 1842
XapI RAATTY 6 cut(s) 616, 1332, 1610, 1757, 2062, 2574
XceI RCATGY 1 cut(s) 3377
XhoI CTCGAG 1 cut(s) 2823
XmnI GAANNNNTTC 2 cut(s) 1761, 3054
XspI CTAG 5 cut(s) 108, 2253, 2426, 3182, 3198
ZraI GACGTC 1 cut(s) 742
ZrmI AGTACT 1 cut(s) 2430
Zsp2I ATGCAT 1 cut(s) 3397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.