MD09G1284300.v1.1

Nodal modulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0011945
Physical Location & Seq
Forward (+)
36190140 .. 36210587
20448 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1284300.v1.1.491

Sequence Viewer

Length: 3609 bp
ATGGCCATCAAAGACCAGGCCTCACCCTTACTCTTCTTTTTCTTCTTCCTCGCAATCTCTTCCCTCGCTACCACTTTCGCCGATTCAGTCCACGGCTGCGGCGGTTTCGTCGAGGCTCGCCATTCTCTGATCAAGGCCAGGAAACTCACCGATGCTAAATTGGATTTTTCTCACATCACGGTTGAGCTTCGGACAGTTGATGGGTTTTTGAAGGATAGCACTCAATGTGCTCCCAATGGTTACTACTTCATTCCCGTATATGACAAGGGCTCTTTTGTTATTAAAATTAACGGCCCAGAGGGATGGTCATGGAACCCAGACAAGGTTCCTGTTACTGTTGATGATTCCGGCTGCAATGGCAGTGAAGATATTAACTTTCGGTTTACAGGGTTCTCCTTGTCTGGTAGAGTAGTGGGAGCGGTTGGTGGAGGGAGTTGTTCCGTTCAGAATGGAGGTCCTCCAAATATTGAAGTTGAACTTTTGTCTGATTCTGGTGGTGTTGTTTCTTCTGTTATAACATCAGCAGGGGGGAGTTACTTGTTCAAAAATATTATTCCAGGAAATTATGAATTACGTGCTTCGCACCCTGACTTGAAAGTTGAAATAAGAGGTTCAACAAAGGTGAATTTGGGGTTCGGAAATGATGTAGTGGATGACATTTTCTTTGTCCCTGGGTATGATATTCGTGGATTTGTTGTTTCTCAGGGAAATCCAATATTGGGAGTTCACGTTTATTTATACTCGGATGACGTCTTAGAGGTAGATTGTCCCCAAGGTTCTGGAACTTCTTCTGGAACGAGGAAGGCACTGTGCCATGCTATATCTGATGCTCATGGGATGTTCATGTTTAAATCAATACCTTGTGGAACGTATGAGCTCATACCTTATTACAAGGGGGAAAACACAGTATTTGATGTTTCACCTCCTGTCATGTCCATAACTGTTGAGCATCAACATGTGACAGTTCCTCAGAAGTTCCAGGTCACTGGATTTTCTGTTGGGGGACGAGTTGTGGATGGAAATGATGAAGGCGTTGAAGGTGTTAAAATTAAAGTTGATGGTCATGAAAGGTCCATCACTGACAAACAAGGATATTACAAGCTTGACCAGGTTACTTCCAATCGCTATGCAATAGAAGCCACAAAGGAGCATTACAAGTTCAGCAGTTTGAAGAACTATTTGGTATTACCAAATATGGCTTCGGTTATGGATATTAAAGCAGTTTCCTATGATGTCTGTGGTGTGGTTCAGATAGTTAGTGCTGGTTACAAGGCAAAGGTAGCTTTGACTCGTGGGCGTGGCCCTGAAAATGTCAAACCGCAAGTGAAACAGACTGATGGAAGTGGAAATTTCTGCTTTGAGGTCCTACCTGGTGACTATCGTTTATCTGCTATAGCAGCTACCCCTGACAGTGCTTCTGGCCTAATGTTTTTGCCGTCTTATATCAATGTTGCTGTCAAAGGTCCATTATTGAACGTCAAGTTTTCTCAGGCATTGGTCAATGTACGTGGAACTGTAACCTGCAAGGAGAAGTGTGGCACATCTGTTTCTGTTATCCTTGTTGGTCTAGCTGGTAAACGTAATGAAGAGAAGAGGACAATTAGTTTGACTGATGAGAGTAGTGATTTTCACTTCGAAAATGTCATCCCAGGGAAATACAGATTTGAGGTCAAACACAATTCAGAAGAATCCACAGCTGTAGAAGATAATTGGTGTTGGGAGAAAAGTTCCATTGATGTGGATGTTGGTGTGGAAGATGTAAAAGGAATTGAATTTGTTCAAAAAGGTTACCGGCTCAATGTCATTGCCACCCATGATGTAGATGCTTATATGATTCAGCCAGATGGTTCTTCCATTAATTTGAAAATTAAGAAAGGTTCCCAGAATATATGTGTAGAATATCCTGGAGTGCATGAACTTCAATTTGTCAACTCGTGTATTTTCTTTGGGAGCTCATCAATTAAGATTGATACTTTAAACCCATCGGCTATTTATCTGAAAGGAGAAAAGTATCTTGTTAAAGGACAAATCAATGTTTCATCAAGCTCATTTGAGGGCGAGGTACCTGAGAATTTCGTTGTGGATATTCTGAATGCCGGGGGCAGTATTATAGATGAGACCACAGCTAGGCTCACTTCCAGTGGAAATGATCAAAGTACTATAGTGTATGAATATTCTGCATGGGCCAATCGCGGAGAAAGACTTACCTTTGTTCCTAAGGATCCAAGAACTGATGAGACGAGGAAGATCTTGTTTTATCCCAGACAATATCATGTCTTAATTACAATTGATGGCTGCCAAGCTTCAATCCCTCCATTCTCTGGTCGATTGGGTTTATATATTACAGGATCAGTCTCTCCCCCTCTTTCTGAAGTTCATATTAAGATTCTTGCTGCTGGGGATAGCCACATTGCTCAACTTAAGGACGGTGAAATAGTACTGGAGACTACCACGGGAATGGATGGCTCTTTTGTTGGGGGACCTTTATATGATGAAGTAACATACCATGTTCAGGCTTCAAAGCCTGGGTATCATTTAAAACAAGTTGGGCCTCATTCCTTTTCTTGTCAGAAGCTTGGCCAAATTTCTATAAATATTCATTCTAAAGATGATGCTAAAGAGACTATTCCTTCAGTGTTATTATCTTTGAGTGGCGATGATGGCTACAGAAACAACTCGGTATCTGGAGCTGGTGGCGCATTCTTATTTAACAACTTATTTCCAGGGACATTCTATCTCCGTCCTCTGCTAAAGGAGTTTGCCTTTTCTCCTCCAGCACTGGCGATAGATCTTGGTTCTGGGGAGTCTAAAGAAGCTATTTTCTTAGCTGCTCGAGTGGCTTACAGTGCTATGGGTGTCGTTACTCTATTGTCTGGCCAACCAAAAGAAGGCGTGTTGGTTGAGGCCAGATCAGAATCAAAAGGCTATTACGAGGAAACAGTGACTGATTCATCTGGAAGTTATCGTTTGAGAGGACTTCTTCCTGACACAACCTATGTAATCAAAGTAGTGAAAAGGGATGGTTTGGGTAGTGCTAAAATTGAGCGTGCATCACCAGAATCTGTTCCCATCAAGGTTGGACACGAGGATATCAAAGGATTAGGTTTTCTGGTTTTTGAACAGCCAGACACAACTGTTTTAAGTTGCCATGTTGAGGGAAAGAGAATAGAGGAACTGTATCCACATCTACTGGTGGAAATCAAATCATCTAGTGACCTTTCTATGGAGTCCGTCTTCCCTCTTCCGCTTTCCAACTTCTTCCAGGTGAAGGACTTACCCAAGGGTAAGCACCTTCTGCAGCTTCGTTCTAGTCTTCCGTCAAGCAGCCATAAGTTTGAATCCGAGGTTATTGAGGTTGATTTAGAGCAGAATACTCGTATACATGTTGGCCCCCTCAGATATGTATTTGAAGAGGATTACCACAAGCAGGAGTTGACTCCGGCGCCAGTTTTCCCTCTTATAGTTGGCGTCTCAGTGATTGCTCTGTTTGCCACCATTCCAAGCTTGAAGGACTTATACCAATCCACGGTGGGAATACCAACACCCGGATTCACTACAACTGCAAAGAAGGAAGTGCGGAGGCCTGTTTTGAGAAAGAAGACTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CarboxypepD_reg

Protein Analysis

1203

Amino Acids

130.99

Weight (kDa)

5.82

Isoelectric Point (pI)

33.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NOMO1-like_1st PF22898 50 - 126 4.4e-32 BOS complex subunit NOMO1-like, first beta sandwich domain
NOMO1-like_2nd PF22904 128 - 220 8.1e-16 BOS complex subunit NOMO1-like, second beta sandwich domain
NOMO_3rd PF23193 235 - 329 1.1e-09 NOMO third transthyretin-like domain
NOMO1-like_2nd PF22904 329 - 389 6.2e-08 BOS complex subunit NOMO1-like, second beta sandwich domain
CarboxypepD_reg PF13620 332 - 405 1.2e-06 Carboxypeptidase regulatory-like domain
NOMO_5th PF23194 411 - 495 2.6e-09 NOMO fifth transthyretin-like domain
NOMO_6th PF23196 499 - 577 5.7e-08 NOMO sixth transthyretin-like domain
Ig_NOMO PF23141 599 - 670 1.7e-24 NOMO Ig-like domain
NOMO_8th PF23660 672 - 776 1.9e-39 NOMO eighth prealbumin-like domain
NOMO1-like_9th PF22902 778 - 850 1.7e-12 BOS complex subunit NOMO1-like, beta sandwich domain
CarboxypepD_reg PF13620 952 - 1012 1.3e-07 Carboxypeptidase regulatory-like domain
DUF7152 PF23662 1044 - 1141 7.9e-44 Domain of unknown function (DUF7152)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011945)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G62360 AT3G62360
fragaria_vesca FvH4_1g27060
malus_domestica MD09G1284300.v1.1 MD17G1277500.v1.1
prunus_persica Prupe.3G010500_v2.0.a1 Prupe.3G010500_v2.0.a1 Prupe.3G010500_v2.0.a1
pyrus_communis pycom17g27510
rosa_chinensis RchiOBHm_Chr3g0487381
rosa_laevigata RLG00000023009
rosa_multiflora Rmu_sc0001955.1_g000004
rosa_roxburghii Rroxscaffold_6G00394840
rosa_rugosa Rorug03G0233600
rosa_samantha Rh3AG281600 Rh3BG317200 Rh3CG315300 Rh3DG313200
rosa_wichuraiana Rw3G024930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 515
AatII GACGTC 1 cut(s) 753
Acc36I ACCTGC 1 cut(s) 1529
Acc65I GGTACC 1 cut(s) 2062
AccB1I GGYRCC 2 cut(s) 2062, 3442
AccB7I CCANNNNNTGG 1 cut(s) 2321
AccBSI CCGCTC 1 cut(s) 419
AccI GTMKAC 1 cut(s) 3379
AccII CGCG 1 cut(s) 2193
AciI CCGC 7 cut(s) 99, 102, 419, 1319, 2193, 3245, 3577
AclWI GGATC 3 cut(s) 2216, 2229, 2356
AcoI YGGCCR 3 cut(s) 3, 2578, 2875
AcsI RAATTY 5 cut(s) 625, 1348, 1772, 2071, 2583
AcuI CTGAAG 2 cut(s) 2391, 2616
AcyI GRCGYC 3 cut(s) 750, 3443, 3468
AfaI GTAC 4 cut(s) 1506, 2064, 2158, 2439
AflII CTTAAG 1 cut(s) 2420
AflIII ACRYGT 2 cut(s) 955, 3382
AjuI GAANNNNNNNTTGG 4 cut(s) 1163, 1183, 1195, 1215
Alw21I GWGCWC 3 cut(s) 232, 879, 1955
Alw26I GTCTC 6 cut(s) 2111, 2231, 2359, 2438, 2615, 3475
AlwI GGATC 3 cut(s) 2216, 2229, 2356
AlwNI CAGNNNCTG 2 cut(s) 2945, 3062
Ama87I CYCGRG 1 cut(s) 2832
ApeKI GCWGC 8 cut(s) 96, 351, 1397, 2295, 2393, 2828, 3298, 3324
ApoI RAATTY 5 cut(s) 625, 1348, 1772, 2071, 2583
ArsI GACNNNNNNTTYG 4 cut(s) 647, 679, 1588, 1620
AseI ATTAAT 1 cut(s) 1857
Asp700I GAANNNNTTC 3 cut(s) 787, 1776, 3063
Asp718I GGTACC 1 cut(s) 2062
AspLEI GCGC 2 cut(s) 2699, 3445
AsuC2I CCSGG 2 cut(s) 2098, 3546
AsuHPI GGTGA 8 cut(s) 15, 139, 634, 912, 1385, 2441, 3045, 3277
AsuII TTCGAA 1 cut(s) 1635
AvaI CYCGRG 1 cut(s) 2832
AvaII GGWCC 5 cut(s) 455, 1071, 1363, 1463, 2480
AxyI CCTNAGG 1 cut(s) 2217
BalI TGGCCA 3 cut(s) 5, 2580, 2877
BamHI GGATCC 1 cut(s) 2221
BanI GGYRCC 2 cut(s) 2062, 3442
BanII GRGCYC 3 cut(s) 272, 879, 1955
BarI GAAGNNNNNNTAC 2 cut(s) 3500, 3532
BauI CACGAG 3 cut(s) 1290, 1933, 3083
BbsI GAAGAC 3 cut(s) 3226, 3305, 3605
Bbv12I GWGCWC 3 cut(s) 232, 879, 1955
BbvI GCAGC 8 cut(s) 83, 338, 1409, 2282, 2380, 2815, 3310, 3336
BceAI ACGGC 3 cut(s) 109, 307, 1420
BcgI CGANNNNNNTGC 2 cut(s) 3356, 3390
BciVI GTATCC 1 cut(s) 3189
BclI TGATCA 2 cut(s) 129, 2149
BcnI CCSGG 2 cut(s) 2098, 3546
BcoDI GTCTC 6 cut(s) 2111, 2231, 2359, 2438, 2615, 3475
BfaI CTAG 4 cut(s) 1568, 2127, 3210, 3309
BfmI CTRYAG 5 cut(s) 1392, 1698, 2160, 2665, 3296
BfoI RGCGCY 1 cut(s) 3446
BfrI CTTAAG 1 cut(s) 2420
BfuAI ACCTGC 1 cut(s) 1529
BfuI GTATCC 1 cut(s) 3189
BglII AGATCT 2 cut(s) 2247, 2788
BisI GCNGC 9 cut(s) 97, 100, 352, 1398, 2296, 2394, 2829, 3299, 3325
BlsI GCNGC 9 cut(s) 98, 101, 353, 1399, 2297, 2395, 2830, 3300, 3326
BmcAI AGTACT 2 cut(s) 2158, 2439
Bme18I GGWCC 5 cut(s) 455, 1071, 1363, 1463, 2480
BmeT110I CYCGRG 1 cut(s) 2832
BmiI GGNNCC 8 cut(s) 314, 327, 1879, 2064, 2223, 2481, 3391, 3444
BmsI GCATC 6 cut(s) 142, 817, 958, 1813, 2602, 3059
BpiI GAAGAC 3 cut(s) 3226, 3305, 3605
BpmI CTGGAG 4 cut(s) 1926, 2462, 2706, 2757
Bpu14I TTCGAA 1 cut(s) 1635
BpuMI CCSGG 2 cut(s) 2098, 3546
BsaAI YACGTR 2 cut(s) 575, 1508
BsaBI GATNNNNATC 1 cut(s) 2914
BsaHI GRCGYC 3 cut(s) 750, 3443, 3468
BsaI GGTCTC 1 cut(s) 2111
BsaXI ACNNNNNCTCC 2 cut(s) 714, 744
Bse118I RCCGGY 1 cut(s) 1791
Bse1I ACTGG 6 cut(s) 991, 2139, 2445, 2784, 3195, 3446
Bse21I CCTNAGG 1 cut(s) 2217
Bse3DI GCAATG 3 cut(s) 361, 1803, 2409
Bse8I GATNNNNATC 1 cut(s) 2914
BseGI GGATG 9 cut(s) 308, 658, 751, 843, 1021, 1644, 1747, 2467, 3025
BseJI GATNNNNATC 1 cut(s) 2914
BseMI GCAATG 3 cut(s) 361, 1803, 2409
BseMII CTCAG 6 cut(s) 716, 983, 1502, 2058, 3409, 3486
BseNI ACTGG 6 cut(s) 991, 2139, 2445, 2784, 3195, 3446
BseRI GAGGAG 1 cut(s) 2760
BseXI GCAGC 8 cut(s) 83, 338, 1409, 2282, 2380, 2815, 3310, 3336
BseYI CCCAGC 1 cut(s) 2396
Bsh1236I CGCG 1 cut(s) 2193
BshNI GGYRCC 2 cut(s) 2062, 3442
BsiHKAI GWGCWC 3 cut(s) 232, 879, 1955
BsiHKCI CYCGRG 1 cut(s) 2832
BsiSI CCGG 5 cut(s) 348, 1792, 2097, 3440, 3546
BslFI GGGAC 5 cut(s) 653, 753, 1017, 2493, 2740
BsmAI GTCTC 6 cut(s) 2111, 2231, 2359, 2438, 2615, 3475
BsmBI CGTCTC 2 cut(s) 2231, 3475
BsmFI GGGAC 5 cut(s) 653, 753, 1017, 2493, 2740
BsmI GAATGC 2 cut(s) 2098, 2699
Bso31I GGTCTC 1 cut(s) 2111
BsoBI CYCGRG 1 cut(s) 2832
Bsp119I TTCGAA 1 cut(s) 1635
Bsp1286I GDGCHC 4 cut(s) 232, 272, 879, 1955
Bsp143I GATC 7 cut(s) 129, 2149, 2221, 2247, 2348, 2788, 2909
BspACI CCGC 7 cut(s) 99, 102, 419, 1319, 2193, 3245, 3577
BspCNI CTCAG 6 cut(s) 715, 982, 1501, 2059, 3408, 3485
BspFNI CGCG 1 cut(s) 2193
BspHI TCATGA 1 cut(s) 1063
BspLI GGNNCC 8 cut(s) 314, 327, 1879, 2064, 2223, 2481, 3391, 3444
BspMAI CTGCAG 1 cut(s) 3300
BspMI ACCTGC 1 cut(s) 1529
BspPI GGATC 3 cut(s) 2216, 2229, 2356
BspT104I TTCGAA 1 cut(s) 1635
BspT107I GGYRCC 2 cut(s) 2062, 3442
BspTI CTTAAG 1 cut(s) 2420
BspTNI GGTCTC 1 cut(s) 2111
BsrBI CCGCTC 1 cut(s) 419
BsrDI GCAATG 3 cut(s) 361, 1803, 2409
BsrFI RCCGGY 1 cut(s) 1791
BsrI ACTGG 6 cut(s) 991, 2139, 2445, 2784, 3195, 3446
BssAI RCCGGY 1 cut(s) 1791
BssMI GATC 7 cut(s) 129, 2149, 2221, 2247, 2348, 2788, 2909
BssNAI GTATAC 1 cut(s) 3380
BssNI GRCGYC 3 cut(s) 750, 3443, 3468
BssSI CACGAG 3 cut(s) 1290, 1933, 3083
BssT1I CCWWGG 2 cut(s) 772, 3279
Bst1107I GTATAC 1 cut(s) 3380
Bst2BI CACGAG 3 cut(s) 1290, 1933, 3083
Bst6I CTCTTC 6 cut(s) 38, 64, 1581, 1586, 3246, 3405
BstACI GRCGYC 3 cut(s) 750, 3443, 3468
BstAFI CTTAAG 1 cut(s) 2420
BstAPI GCANNNNNTGC 1 cut(s) 3295
BstBAI YACGTR 2 cut(s) 575, 1508
BstBI TTCGAA 1 cut(s) 1635
BstC8I GCNNGC 2 cut(s) 118, 3048
BstDEI CTNAG 9 cut(s) 702, 754, 969, 1488, 2067, 2217, 2824, 3395, 3472
BstDSI CCRYGG 3 cut(s) 91, 2451, 3525
BstEII GGTNACC 1 cut(s) 1787
BstF5I GGATG 9 cut(s) 308, 658, 751, 843, 1021, 1644, 1747, 2467, 3025
BstFNI CGCG 1 cut(s) 2193
BstH2I RGCGCY 1 cut(s) 3446
BstHHI GCGC 2 cut(s) 2699, 3445
BstKTI GATC 7 cut(s) 132, 2152, 2224, 2250, 2351, 2791, 2912
BstMAI GTCTC 6 cut(s) 2111, 2231, 2359, 2438, 2615, 3475
BstMBI GATC 7 cut(s) 129, 2149, 2221, 2247, 2348, 2788, 2909
BstNSI RCATGY 2 cut(s) 959, 3386
BstPI GGTNACC 1 cut(s) 1787
BstSFI CTRYAG 5 cut(s) 1392, 1698, 2160, 2665, 3296
BstUI CGCG 1 cut(s) 2193
BstV1I GCAGC 8 cut(s) 83, 338, 1409, 2282, 2380, 2815, 3310, 3336
BstV2I GAAGAC 3 cut(s) 3226, 3305, 3605
BstX2I RGATCY 3 cut(s) 2221, 2247, 2788
BstXI CCANNNNNNTGG 5 cut(s) 303, 779, 986, 1738, 2458
BstYI RGATCY 3 cut(s) 2221, 2247, 2788
BstZ17I GTATAC 1 cut(s) 3380
Bsu36I CCTNAGG 1 cut(s) 2217
BsuI GTATCC 1 cut(s) 3189
BtgI CCRYGG 3 cut(s) 91, 2451, 3525
BtgZI GCGATG 1 cut(s) 2670
BtsCI GGATG 9 cut(s) 308, 658, 751, 843, 1021, 1644, 1747, 2467, 3025
BtsI GCAGTG 1 cut(s) 367
BveI ACCTGC 1 cut(s) 1529
Cac8I GCNNGC 2 cut(s) 118, 3048
CaiI CAGNNNCTG 2 cut(s) 2945, 3062
CciI TCATGA 1 cut(s) 1063
CfoI GCGC 2 cut(s) 2699, 3445
Cfr10I RCCGGY 1 cut(s) 1791
CseI GACGC 1 cut(s) 3457
CsiI ACCWGGT 2 cut(s) 1107, 1369
Csp6I GTAC 4 cut(s) 1505, 2063, 2157, 2438
CspCI CAANNNNNGTGG 2 cut(s) 1489, 1524
CviQI GTAC 4 cut(s) 1505, 2063, 2157, 2438
DdeI CTNAG 9 cut(s) 702, 754, 969, 1488, 2067, 2217, 2824, 3395, 3472
DinI GGCGCC 1 cut(s) 3444
DpnI GATC 7 cut(s) 131, 2151, 2223, 2249, 2350, 2790, 2911
DpnII GATC 7 cut(s) 129, 2149, 2221, 2247, 2348, 2788, 2909
DraI TTTAAA 3 cut(s) 850, 1977, 2538
EaeI YGGCCR 3 cut(s) 3, 2578, 2875
Eam1104I CTCTTC 6 cut(s) 38, 64, 1581, 1586, 3246, 3405
EarI CTCTTC 6 cut(s) 38, 64, 1581, 1586, 3246, 3405
Ecl136II GAGCTC 2 cut(s) 877, 1953
Eco130I CCWWGG 2 cut(s) 772, 3279
Eco147I AGGCCT 2 cut(s) 20, 3583
Eco24I GRGCYC 3 cut(s) 272, 879, 1955
Eco31I GGTCTC 1 cut(s) 2111
Eco32I GATATC 1 cut(s) 3091
Eco47I GGWCC 5 cut(s) 455, 1071, 1363, 1463, 2480
Eco53kI GAGCTC 2 cut(s) 877, 1953
Eco57I CTGAAG 2 cut(s) 2391, 2616
Eco81I CCTNAGG 1 cut(s) 2217
Eco88I CYCGRG 1 cut(s) 2832
Eco91I GGTNACC 1 cut(s) 1787
EcoICRI GAGCTC 2 cut(s) 877, 1953
EcoO109I RGGNCCY 3 cut(s) 455, 1363, 2480
EcoO65I GGTNACC 1 cut(s) 1787
EcoRV GATATC 1 cut(s) 3091
EcoT14I CCWWGG 2 cut(s) 772, 3279
EcoT38I GRGCYC 3 cut(s) 272, 879, 1955
EgeI GGCGCC 1 cut(s) 3444
EheI GGCGCC 1 cut(s) 3444
ErhI CCWWGG 2 cut(s) 772, 3279
Esp3I CGTCTC 2 cut(s) 2231, 3475
FalI AAGNNNNNCTT 2 cut(s) 462, 494
FaqI GGGAC 5 cut(s) 653, 753, 1017, 2493, 2740
FbaI TGATCA 2 cut(s) 129, 2149
FblI GTMKAC 1 cut(s) 3379
Fnu4HI GCNGC 9 cut(s) 97, 100, 352, 1398, 2296, 2394, 2829, 3299, 3325
FokI GGATG 9 cut(s) 315, 665, 758, 850, 1028, 1631, 1754, 2474, 3032
FriOI GRGCYC 3 cut(s) 272, 879, 1955
Fsp4HI GCNGC 9 cut(s) 97, 100, 352, 1398, 2296, 2394, 2829, 3299, 3325
FspBI CTAG 4 cut(s) 1568, 2127, 3210, 3309
GlaI GCGC 2 cut(s) 2698, 3444
GluI GCNGC 9 cut(s) 97, 100, 352, 1398, 2296, 2394, 2829, 3299, 3325
GsaI CCCAGC 1 cut(s) 2400
GsuI CTGGAG 4 cut(s) 1926, 2462, 2706, 2757
HaeII RGCGCY 1 cut(s) 3446
HapII CCGG 5 cut(s) 348, 1792, 2097, 3440, 3546
HgaI GACGC 1 cut(s) 3457
HhaI GCGC 2 cut(s) 2699, 3445
Hin1I GRCGYC 3 cut(s) 750, 3443, 3468
Hin6I GCGC 2 cut(s) 2697, 3443
HinP1I GCGC 2 cut(s) 2697, 3443
HincII GTYRAC 2 cut(s) 1930, 3435
HindII GTYRAC 2 cut(s) 1930, 3435
HindIII AAGCTT 4 cut(s) 1100, 2301, 2573, 3502
HpaII CCGG 5 cut(s) 348, 1792, 2097, 3440, 3546
HphI GGTGA 8 cut(s) 15, 139, 634, 912, 1385, 2441, 3045, 3277
Hpy166II GTNNAC 7 cut(s) 91, 384, 727, 1577, 1930, 3380, 3435
Hpy188III TCNNGA 6 cut(s) 780, 792, 1064, 2685, 2955, 2984
Hpy8I GTNNAC 7 cut(s) 91, 384, 727, 1577, 1930, 3380, 3435
Hpy99I CGWCG 1 cut(s) 113
HpyCH4IV ACGT 7 cut(s) 574, 729, 750, 869, 1476, 1507, 1579
HpyCH4V TGCA 8 cut(s) 354, 1130, 1524, 1912, 2180, 3050, 3298, 3563
HpyF3I CTNAG 9 cut(s) 702, 754, 969, 1488, 2067, 2217, 2824, 3395, 3472
HpySE526I ACGT 7 cut(s) 574, 729, 750, 869, 1476, 1507, 1579
Hsp92I GRCGYC 3 cut(s) 750, 3443, 3468
HspAI GCGC 2 cut(s) 2697, 3443
KasI GGCGCC 1 cut(s) 3442
KpnI GGTACC 1 cut(s) 2066
Ksp22I TGATCA 2 cut(s) 129, 2149
Kzo9I GATC 7 cut(s) 129, 2149, 2221, 2247, 2348, 2788, 2909
LmnI GCTCC 5 cut(s) 235, 416, 1147, 1950, 2687
Lsp1109I GCAGC 8 cut(s) 83, 338, 1409, 2282, 2380, 2815, 3310, 3336
LweI GCATC 6 cut(s) 142, 817, 958, 1813, 2602, 3059
MabI ACCWGGT 2 cut(s) 1107, 1369
MaeI CTAG 4 cut(s) 1568, 2127, 3210, 3309
MaeII ACGT 7 cut(s) 574, 729, 750, 869, 1476, 1507, 1579
MalI GATC 7 cut(s) 131, 2151, 2223, 2249, 2350, 2790, 2911
MbiI CCGCTC 1 cut(s) 419
MboI GATC 7 cut(s) 129, 2149, 2221, 2247, 2348, 2788, 2909
MfeI CAATTG 1 cut(s) 2286
MflI RGATCY 3 cut(s) 2221, 2247, 2788
MhlI GDGCHC 4 cut(s) 232, 272, 879, 1955
MlsI TGGCCA 3 cut(s) 5, 2580, 2877
MluNI TGGCCA 3 cut(s) 5, 2580, 2877
Mly113I GGCGCC 1 cut(s) 3443
MlyI GAGTC 4 cut(s) 1282, 2813, 3236, 3430
MmeI TCCRAC 2 cut(s) 3058, 3276
Mox20I TGGCCA 3 cut(s) 5, 2580, 2877
MroXI GAANNNNTTC 3 cut(s) 787, 1776, 3063
MscI TGGCCA 3 cut(s) 5, 2580, 2877
MslI CAYNNNNRTG 3 cut(s) 954, 1736, 2456
Msp20I TGGCCA 3 cut(s) 5, 2580, 2877
MspA1I CMGCKG 1 cut(s) 1697
MspCI CTTAAG 1 cut(s) 2420
MspI CCGG 5 cut(s) 348, 1792, 2097, 3440, 3546
MunI CAATTG 1 cut(s) 2286
Mva1269I GAATGC 2 cut(s) 2098, 2699
MvnI CGCG 1 cut(s) 2193
NarI GGCGCC 1 cut(s) 3443
NciI CCSGG 2 cut(s) 2098, 3546
NdeII GATC 7 cut(s) 129, 2149, 2221, 2247, 2348, 2788, 2909
NlaIV GGNNCC 8 cut(s) 314, 327, 1879, 2064, 2223, 2481, 3391, 3444
NmuCI GTSAC 5 cut(s) 958, 982, 1373, 2941, 3212
NspI RCATGY 2 cut(s) 959, 3386
NspV TTCGAA 1 cut(s) 1635
PaeR7I CTCGAG 1 cut(s) 2832
PagI TCATGA 1 cut(s) 1063
PasI CCCWGGG 2 cut(s) 671, 1649
PceI AGGCCT 2 cut(s) 20, 3583
PciI ACATGT 2 cut(s) 955, 3382
PctI GAATGC 2 cut(s) 2098, 2699
PdmI GAANNNNTTC 3 cut(s) 787, 1776, 3063
PflMI CCANNNNNTGG 1 cut(s) 2321
PfoI TCCNGGA 2 cut(s) 556, 1903
PkrI GCNGC 9 cut(s) 98, 101, 353, 1399, 2297, 2395, 2830, 3300, 3326
PleI GAGTC 4 cut(s) 1282, 2812, 3235, 3430
PluTI GGCGCC 1 cut(s) 3446
PpsI GAGTC 4 cut(s) 1282, 2812, 3235, 3430
Ppu21I YACGTR 2 cut(s) 575, 1508
PpuMI RGGWCCY 3 cut(s) 455, 1363, 2480
PscI ACATGT 2 cut(s) 955, 3382
PshBI ATTAAT 1 cut(s) 1857
PsiI TTATAA 1 cut(s) 515
Psp124BI GAGCTC 2 cut(s) 879, 1955
Psp5II RGGWCCY 3 cut(s) 455, 1363, 2480
PspEI GGTNACC 1 cut(s) 1787
PspFI CCCAGC 1 cut(s) 2396
PspN4I GGNNCC 8 cut(s) 314, 327, 1879, 2064, 2223, 2481, 3391, 3444
PspPPI RGGWCCY 3 cut(s) 455, 1363, 2480
PspXI VCTCGAGB 1 cut(s) 2832
PstI CTGCAG 1 cut(s) 3300
PstNI CAGNNNCTG 2 cut(s) 2945, 3062
PsuI RGATCY 3 cut(s) 2221, 2247, 2788
PvuII CAGCTG 1 cut(s) 1697
RsaI GTAC 4 cut(s) 1506, 2064, 2158, 2439
RsaNI GTAC 4 cut(s) 1505, 2063, 2157, 2438
RseI CAYNNNNRTG 3 cut(s) 954, 1736, 2456
SacI GAGCTC 2 cut(s) 879, 1955
SatI GCNGC 9 cut(s) 97, 100, 352, 1398, 2296, 2394, 2829, 3299, 3325
Sau3AI GATC 7 cut(s) 129, 2149, 2221, 2247, 2348, 2788, 2909
ScaI AGTACT 2 cut(s) 2158, 2439
SchI GAGTC 4 cut(s) 1282, 2813, 3236, 3430
SduI GDGCHC 4 cut(s) 232, 272, 879, 1955
SexAI ACCWGGT 2 cut(s) 1107, 1369
SfaNI GCATC 6 cut(s) 142, 817, 958, 1813, 2602, 3059
SfcI CTRYAG 5 cut(s) 1392, 1698, 2160, 2665, 3296
SfoI GGCGCC 1 cut(s) 3444
Sfr274I CTCGAG 1 cut(s) 2832
SfuI TTCGAA 1 cut(s) 1635
SinI GGWCC 5 cut(s) 455, 1071, 1363, 1463, 2480
SlaI CTCGAG 1 cut(s) 2832
SmiMI CAYNNNNRTG 3 cut(s) 954, 1736, 2456
SmlI CTYRAG 2 cut(s) 2420, 2832
SmoI CTYRAG 2 cut(s) 2420, 2832
SseBI AGGCCT 2 cut(s) 20, 3583
SsiI CCGC 7 cut(s) 99, 102, 419, 1319, 2193, 3245, 3577
SspDI GGCGCC 1 cut(s) 3442
SspI AATATT 5 cut(s) 466, 550, 717, 2174, 2596
SspMI CTAG 4 cut(s) 1568, 2127, 3210, 3309
SstI GAGCTC 2 cut(s) 879, 1955
StuI AGGCCT 2 cut(s) 20, 3583
StyI CCWWGG 2 cut(s) 772, 3279
TaiI ACGT 7 cut(s) 577, 732, 753, 872, 1479, 1510, 1582
TaqI TCGA 4 cut(s) 111, 1635, 2326, 2833
TatI WGTACW 2 cut(s) 2156, 2437
TauI GCSGC 1 cut(s) 102
TseFI GTSAC 5 cut(s) 958, 982, 1373, 2941, 3212
TseI GCWGC 8 cut(s) 96, 351, 1397, 2295, 2393, 2828, 3298, 3324
Tsp45I GTSAC 5 cut(s) 958, 982, 1373, 2941, 3212
TspGWI ACGGA 4 cut(s) 430, 2729, 3220, 3306
Van91I CCANNNNNTGG 1 cut(s) 2321
Vha464I CTTAAG 1 cut(s) 2420
VpaK11BI GGWCC 5 cut(s) 455, 1071, 1363, 1463, 2480
VspI ATTAAT 1 cut(s) 1857
XapI RAATTY 5 cut(s) 625, 1348, 1772, 2071, 2583
XceI RCATGY 2 cut(s) 959, 3386
XhoI CTCGAG 1 cut(s) 2832
XmiI GTMKAC 1 cut(s) 3379
XmnI GAANNNNTTC 3 cut(s) 787, 1776, 3063
XspI CTAG 4 cut(s) 1568, 2127, 3210, 3309
ZraI GACGTC 1 cut(s) 751
ZrmI AGTACT 2 cut(s) 2158, 2439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.