Rw3G024930

Nodal modulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0011945
Physical Location & Seq
Reverse (-)
32561319 .. 32571897
10579 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G024930.1

Sequence Viewer

Length: 3570 bp
ATGGGCCTCAAACTCTTACTCTTCGTCTTCGCGGTTACCTCACTGATCTCCACCGCCGCCTTCGCCGATTCAATCCACGGTTGCGGTGGCTTCGTCGAGGCGAGCTCTTCTTTGATCAAGGCCAGGAAGCCCTCCGATGCTAAATTCGACTATTCTCACATCACGGTTGAGCTTCGCACGGTTGACGGTTTGTTGAAGGAGAGTACTCAGTGCTCTCCCAATGGCTACTACTTCATCCCCGTCTATGACAAGGGTTCTTTTGTGATTAAAATCAACGGACCAGATGGATGGTCATTGGACCCTGATAAGGTTCCTGTTGTTGTTGATCACACTGGCTGCAATGGCAGTGAGGATATTAATTTTCGGTTTACTGGGTTCTCCGTGTCTGGTAGAGTTCTGGGAGCTGTTGGTGGAGAGAGCTGTGCCCTTCAAAATGGAGGCCCCTCAGACATCAAAGTTGAACTTTTGTCAGACAGTGGTGATGTTGTTTCTTCTGTTTCAACATCGTCAGGGGGAAGTTACTTATTCAAGAATATTATTCCAGGAAAATATGAAATACGTGCTTCACATCCCGACTTGAAAGTTGAAATAAGAGGTTCAACAGAGGTGAATGTGGGATTTGGAAACAGCGTGGTTGACGACATTTTCTTTGTTCCTGGCTATGACATAAGTGGATTTGTTGTTTCTCAGGGGAATCCGATATTGGGAGTTCATGTTTATTTACACTCAGATGATGTCTTAGAGGTAGATTGTCCCCAAGGTTCTGGTACAGGCTCTGAAATGAAGAAGGCTCTTTGCCACGCCGTATCTGATGCACAAGGAAAATTCATGTTCAAGTCGCTACCTTGTGGAACTTATGAGCTCATACCTTACTACAAGGGGGAAAACACAGTCTTTGATGTTTCACCTCCTGTCATGTCAGTAACTGTTGAGCATCAACATGTGACAGTTCTGCAAAAGTTCCAGGTCACTGGATTCTCTGTTGGAGGGCGTGTGGTTGACGGAAACAACATGGGGGTTGAAGGTGTTAAAATTATAGTTGATGGTCATGAAAGGTCTGTCACTGACAAACAAGGTTATTACAAGCTTGACCAGGTTACTTCCAATCGCTATACAATAGAAGCCACAAAGGAGCATTACAAGTTCAGTAAACTAACCGATTATTTGGTATTACCAAACATGGCGTCTATAGTGGATATTAAAGCAGTTTCCTATGATGTCTGCGGTGCGGTGCAGATGGTTAGTGCTGGTTACAAGGCAAAGGTAGCTTTGACTCATGGACCTGAAAATGTCAAACCGCAAGTGAAACAGACCAATGGAAACGGAAATTTCTGCTTCGAGGTTCCAACTGGTGAATATCGTTTATCTGCTTTAGCAGCTTCACCTGAGAGTGCTTCTGGTCTACTGTTTTTGCCATCTCATATCGATGTTGTTGTCAAAAGTCCGTTATTGAATGTCAAATTTTCTCAGGCACTAGTTACAGTGCGTGGTACTGTAGCCTGCAAGGAGAAGTGTGGCACATCTGTTTCTGTTACCCTTAACAGTATAGGTGGTAAGCGTAATGAAGAGAGGAAAACAGTTAGTTTGACTGATGAGAGTAGTGAATTTGTCTTTCATAACGTCATCCCTGGAAAATACAGAGTTGAGGTTAAACACAATTCCCGAGAATCTGTTAGTGGAGAAGATAATTGGTGCTGGGAAAAAAGCTTGATTGATGTGGATGTTGGTGTTGACGACGTAGAAGGAATTGAATTTGTCCAGAAAGGTTATTGGGTCCATGTCATTTCCACCCATGATGTAGATGCTTCTATGGTTCATCCAGATGGTTCATCCATGAAATTAAAAATTAAGAAAGGTTCCCAGAATATATGTGTAGAACATCCTGGAGTACATCAACTTCACTTTGTCAACTCTTGTATTTTCTTTGGAAGCTCAGCAATTCAGATTGATACATCAAACCCATCGCCTATTCATCTAAAAGGAGAAAAGTACCTTCTTAAAGGACAAATCAATGTTGCATCAAGCTCATTTGATGGTGGGAACAAGTTATCTGAGAATTTCATTGTGGACATTCTGAATACTGGGGGAAGTATTATAGATAGTGCAACAGCTGGACTTACTTCCACTGGGAATGAGCAAGCAAGTTATTCTGTGTATGAATTCTCTGTATGGGCCAATCTTGGAGAGAAACTTACCTTTGTTCCTCGGGATCCAAGAAATAATGAGATGGGGAAGATATTGTTTTATCCCAGACAACATCATGTCTTGGTTAAAAATGATGGTTGTCAAGCTATGATCCCTGCATTTAATGGTCGATTGGGTTTATATATTGAAGGATCAGTGTCTCCCCCTCTTTCTGATGTTCATATTAAGATTATTGCTGCTGGGGAAAGCCACATTGCGCAACTTAAGGAAGGGGAATTAGTTATTGAAACTACCACAGCTATGGATGGCTCGTTTGTTGGGGGGCCTTTATATGATGATATAACATACAATGTTGAGGCTTCAAAGCTTGGCTACCATTTAAAACAAGTTGGGCCCCATTCCTTTTCTTGTCAGAAGCTTGGCCAAATTTCTGTAGAAATATATTCCAAAGATGACGCCAAAGATGGCGACGATGGCTACAGAAACAACTCAATATCTGGAGCTGGTGGAGCATTCCTCTTTAATAACTTATTTCCTGGAACTTTCTATTTGCGTCCTCTGCTAAAGGAGTTTGCCTTCTCACCTCCAGCACAGGCTATTGATCTTGGCTCCGGGGAGTCTAAAGCAGCTATTTTCCAGGCCACTCGCGTTGCTTACAGTGCCATGGGCATGGTCGCTTTGTTGTCCGGCCAACCAATAGAAGGTGTCTTGATCGAGGCCAGATCAGAATCAAAAGGCTTCTACGAGGAAACAGTGACTGACTCATCTGGAAGTTATCGTTTGAGAGGACTTCTTCCTGACACAACCTATGTAATCAAAGTAGTAAAAAGGGATGGTTTGGGTAGCAGTAAAATTGAGAGGGCATCACCAGACTCGGTTCCAGTTAATGTTGGGTATGAAGATATCAAAGGATTAGACTTTCTGGTGTTTGAACAGCCAGACACAACTATTTTAAGTTGCCATGTTGAAGGAATGAAGAATGAGGAACTCCACTCGCATCTACTGGTGGAAATCAAATCATCTAGTGACAATTCTAGGATCGAGTCCGTCTTCCCTCTTCCACTTTCGAACTTTTTCCAGGTGAAGGACTTACCCAAGGGTAAGCATCTTCTGCAACTCCGGTCTAGTCTTCCATCTAGCAGCCACAAATTTGAATCCGAGATTATTGAGGTCGATTTTGAGAAGAATGCTCATATACATGTTGGCCCACTCAAATACGTTTATGAAGAGGATCACCAGAAGCAGGATTTGACTCCGGCACCAGTTTTTCCTCTTATCGTAGGAGTCTCGGTGATTGCTCTATTTCTTACTATCCCAAGGTTGAACGATTTGTATCAGTCCATGATTGGAACACCAACACCTGGATTCACTACAACTGCAAAGAAGGAAGTGAGGAAGCCCATGTTGAGAAAAAAGACGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CarboxypepD_reg

Protein Analysis

1189

Amino Acids

129.22

Weight (kDa)

5.76

Isoelectric Point (pI)

31.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NOMO1-like_1st PF22898 45 - 121 3.6e-31 BOS complex subunit NOMO1-like, first beta sandwich domain
NOMO1-like_2nd PF22904 123 - 216 1.1e-18 BOS complex subunit NOMO1-like, second beta sandwich domain
SdrD_B PF17210 147 - 192 6.9e-06 SdrD B-like domain
NOMO_3rd PF23193 261 - 324 9.8e-11 NOMO third transthyretin-like domain
NOMO1-like_2nd PF22904 324 - 384 1.6e-07 BOS complex subunit NOMO1-like, second beta sandwich domain
CarboxypepD_reg PF13620 327 - 400 4e-07 Carboxypeptidase regulatory-like domain
NOMO_5th PF23194 406 - 488 6.3e-10 NOMO fifth transthyretin-like domain
NOMO_6th PF23196 497 - 569 2.5e-07 NOMO sixth transthyretin-like domain
Ig_NOMO PF23141 591 - 663 2.6e-27 NOMO Ig-like domain
NOMO_8th PF23660 665 - 772 3.1e-41 NOMO eighth prealbumin-like domain
NOMO1-like_9th PF22902 774 - 846 1.1e-13 BOS complex subunit NOMO1-like, beta sandwich domain
CarboxypepD_reg PF13620 938 - 998 3.1e-08 Carboxypeptidase regulatory-like domain
DUF7152 PF23662 1030 - 1128 1.9e-46 Domain of unknown function (DUF7152)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011945)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G62360 AT3G62360
fragaria_vesca FvH4_1g27060
malus_domestica MD09G1284300.v1.1 MD17G1277500.v1.1
prunus_persica Prupe.3G010500_v2.0.a1 Prupe.3G010500_v2.0.a1 Prupe.3G010500_v2.0.a1
pyrus_communis pycom17g27510
rosa_chinensis RchiOBHm_Chr3g0487381
rosa_laevigata RLG00000023009
rosa_multiflora Rmu_sc0001955.1_g000004
rosa_roxburghii Rroxscaffold_6G00394840
rosa_rugosa Rorug03G0233600
rosa_samantha Rh3AG281600 Rh3BG317200 Rh3CG315300 Rh3DG313200
rosa_wichuraiana Rw3G024930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2403
AccB1I GGYRCC 1 cut(s) 3403
AccB7I CCANNNNNTGG 1 cut(s) 3506
AccI GTMKAC 1 cut(s) 1402
AccII CGCG 2 cut(s) 32, 2793
AciI CCGC 7 cut(s) 32, 54, 57, 84, 1224, 1229, 1298
AclWI GGATC 6 cut(s) 2204, 2217, 2290, 2344, 3191, 3384
AcoI YGGCCR 2 cut(s) 2566, 2833
AcyI GRCGYC 2 cut(s) 1184, 2601
AfaI GTAC 6 cut(s) 205, 769, 1492, 1890, 1991, 3566
AfiI CCNNNNNNNGG 6 cut(s) 307, 704, 2846, 3229, 3388, 3506
AflII CTTAAG 1 cut(s) 2408
AflIII ACRYGT 2 cut(s) 940, 3343
AhlI ACTAGT 1 cut(s) 1474
AjuI GAANNNNNNNTTGG 2 cut(s) 686, 718
Alw21I GWGCWC 3 cut(s) 107, 215, 864
Alw26I GTCTC 2 cut(s) 2349, 3436
AlwI GGATC 6 cut(s) 2204, 2217, 2290, 2344, 3191, 3384
AlwNI CAGNNNCTG 3 cut(s) 776, 926, 2903
Ama87I CYCGRG 2 cut(s) 1662, 2205
ApaI GGGCCC 1 cut(s) 2541
ApeKI GCWGC 5 cut(s) 336, 1376, 2381, 2771, 3285
ArsI GACNNNNNNTTYG 4 cut(s) 632, 664, 1169, 1201
AseI ATTAAT 1 cut(s) 357
Asp700I GAANNNNTTC 1 cut(s) 3218
AspLEI GCGC 1 cut(s) 2404
AsuC2I CCSGG 1 cut(s) 2758
AsuII TTCGAA 1 cut(s) 3212
AvaI CYCGRG 2 cut(s) 1662, 2205
AvaII GGWCC 4 cut(s) 278, 298, 1280, 1774
BaeGI GKGCMC 2 cut(s) 427, 2541
BalI TGGCCA 1 cut(s) 2568
BamHI GGATCC 1 cut(s) 2209
BanI GGYRCC 1 cut(s) 3403
BanII GRGCYC 3 cut(s) 107, 864, 2541
BarI GAAGNNNNNNTAC 2 cut(s) 1881, 1913
BbsI GAAGAC 3 cut(s) 19, 3187, 3266
Bbv12I GWGCWC 3 cut(s) 107, 215, 864
BbvI GCAGC 5 cut(s) 323, 1388, 2368, 2783, 3297
BceAI ACGGC 1 cut(s) 788
BclI TGATCA 2 cut(s) 114, 325
BcnI CCSGG 1 cut(s) 2758
BcoDI GTCTC 2 cut(s) 2349, 3436
BcuI ACTAGT 1 cut(s) 1474
BfaI CTAG 5 cut(s) 1475, 3168, 3180, 3270, 3282
BfmI CTRYAG 4 cut(s) 1188, 1494, 2577, 2623
BfrI CTTAAG 1 cut(s) 2408
BisI GCNGC 6 cut(s) 57, 337, 1377, 2382, 2772, 3286
BlpI GCTNAGC 1 cut(s) 1933
BlsI GCNGC 6 cut(s) 58, 338, 1378, 2383, 2773, 3287
BmcAI AGTACT 1 cut(s) 205
Bme18I GGWCC 4 cut(s) 278, 298, 1280, 1774
BmeT110I CYCGRG 2 cut(s) 1662, 2205
BmrI ACTGGG 3 cut(s) 381, 2091, 2136
BmsI GCATC 8 cut(s) 127, 802, 943, 1792, 2027, 3017, 3151, 3259
BmuI ACTGGG 3 cut(s) 381, 2091, 2136
BpiI GAAGAC 3 cut(s) 19, 3187, 3266
BplI GAGNNNNNCTC 4 cut(s) 89, 121, 2646, 2678
BpmI CTGGAG 3 cut(s) 1905, 2664, 2715
Bpu1102I GCTNAGC 1 cut(s) 1933
Bpu14I TTCGAA 1 cut(s) 3212
BpuMI CCSGG 1 cut(s) 2758
Bsa29I ATCGAT 1 cut(s) 1425
BsaAI YACGTR 1 cut(s) 560
BsaBI GATNNNNATC 3 cut(s) 269, 2872, 3477
BsaHI GRCGYC 2 cut(s) 1184, 2601
BsaJI CCNNGG 8 cut(s) 76, 757, 1627, 2204, 2757, 2808, 3240, 3461
BsaWI WCCGGW 1 cut(s) 3264
BsaXI ACNNNNNCTCC 6 cut(s) 699, 729, 978, 1008, 3420, 3450
Bsc4I CCNNNNNNNGG 6 cut(s) 307, 704, 2846, 3229, 3388, 3506
Bse1I ACTGG 9 cut(s) 337, 376, 976, 1354, 2086, 2131, 3026, 3153, 3407
Bse3DI GCAATG 2 cut(s) 346, 2397
Bse8I GATNNNNATC 3 cut(s) 269, 2872, 3477
BseCI ATCGAT 1 cut(s) 1425
BseDI CCNNGG 8 cut(s) 76, 757, 1627, 2204, 2757, 2808, 3240, 3461
BseJI GATNNNNATC 3 cut(s) 269, 2872, 3477
BseLI CCNNNNNNNGG 6 cut(s) 307, 704, 2846, 3229, 3388, 3506
BseMI GCAATG 2 cut(s) 346, 2397
BseMII CTCAG 8 cut(s) 221, 459, 701, 741, 1377, 1481, 1947, 2043
BseNI ACTGG 9 cut(s) 337, 376, 976, 1354, 2086, 2131, 3026, 3153, 3407
BseSI GKGCMC 2 cut(s) 427, 2541
BseXI GCAGC 5 cut(s) 323, 1388, 2368, 2783, 3297
BseYI CCCAGC 2 cut(s) 1695, 2384
BsgI GTGCAG 1 cut(s) 1253
Bsh1236I CGCG 2 cut(s) 32, 2793
BshNI GGYRCC 1 cut(s) 3403
BshVI ATCGAT 1 cut(s) 1425
BsiHKAI GWGCWC 3 cut(s) 107, 215, 864
BsiHKCI CYCGRG 2 cut(s) 1662, 2205
BsiSI CCGG 4 cut(s) 2757, 2832, 3265, 3401
BslFI GGGAC 1 cut(s) 738
BslI CCNNNNNNNGG 6 cut(s) 307, 704, 2846, 3229, 3388, 3506
BsmAI GTCTC 2 cut(s) 2349, 3436
BsmFI GGGAC 1 cut(s) 738
BsmI GAATGC 2 cut(s) 2657, 3337
BsoBI CYCGRG 2 cut(s) 1662, 2205
Bsp119I TTCGAA 1 cut(s) 3212
Bsp120I GGGCCC 1 cut(s) 2537
Bsp1286I GDGCHC 5 cut(s) 107, 215, 427, 864, 2541
Bsp1720I GCTNAGC 1 cut(s) 1933
Bsp19I CCATGG 1 cut(s) 2808
BspACI CCGC 7 cut(s) 32, 54, 57, 84, 1224, 1229, 1298
BspCNI CTCAG 8 cut(s) 220, 458, 700, 740, 1378, 1480, 1946, 2044
BspDI ATCGAT 1 cut(s) 1425
BspFNI CGCG 2 cut(s) 32, 2793
BspHI TCATGA 1 cut(s) 1048
BspPI GGATC 6 cut(s) 2204, 2217, 2290, 2344, 3191, 3384
BspQI GCTCTTC 1 cut(s) 112
BspT104I TTCGAA 1 cut(s) 3212
BspT107I GGYRCC 1 cut(s) 3403
BspTI CTTAAG 1 cut(s) 2408
BsrDI GCAATG 2 cut(s) 346, 2397
BsrI ACTGG 9 cut(s) 337, 376, 976, 1354, 2086, 2131, 3026, 3153, 3407
BssECI CCNNGG 8 cut(s) 76, 757, 1627, 2204, 2757, 2808, 3240, 3461
BssNI GRCGYC 2 cut(s) 1184, 2601
BssT1I CCWWGG 4 cut(s) 757, 2808, 3240, 3461
Bst6I CTCTTC 5 cut(s) 26, 112, 1560, 3207, 3366
BstACI GRCGYC 2 cut(s) 1184, 2601
BstAFI CTTAAG 1 cut(s) 2408
BstAPI GCANNNNNTGC 1 cut(s) 3256
BstBAI YACGTR 1 cut(s) 560
BstBI TTCGAA 1 cut(s) 3212
BstC8I GCNNGC 3 cut(s) 103, 1501, 2139
BstDEI CTNAG 9 cut(s) 207, 445, 687, 727, 739, 1386, 1467, 1933, 2052
BstDSI CCRYGG 2 cut(s) 76, 2808
BstEII GGTNACC 1 cut(s) 34
BstFNI CGCG 2 cut(s) 32, 2793
BstHHI GCGC 1 cut(s) 2404
BstMAI GTCTC 2 cut(s) 2349, 3436
BstMWI GCNNNNNNNGC 9 cut(s) 62, 342, 1265, 1376, 2619, 2654, 2704, 2804, 3256
BstNSI RCATGY 2 cut(s) 944, 3347
BstPI GGTNACC 1 cut(s) 34
BstSFI CTRYAG 4 cut(s) 1188, 1494, 2577, 2623
BstSLI GKGCMC 2 cut(s) 427, 2541
BstUI CGCG 2 cut(s) 32, 2793
BstV1I GCAGC 5 cut(s) 323, 1388, 2368, 2783, 3297
BstV2I GAAGAC 3 cut(s) 19, 3187, 3266
BstX2I RGATCY 1 cut(s) 2209
BstXI CCANNNNNNTGG 5 cut(s) 288, 764, 971, 2446, 2815
BstYI RGATCY 1 cut(s) 2209
Bsu15I ATCGAT 1 cut(s) 1425
BsuTUI ATCGAT 1 cut(s) 1425
BtgI CCRYGG 2 cut(s) 76, 2808
BtgZI GCGATG 1 cut(s) 1947
BtsI GCAGTG 1 cut(s) 352
Cac8I GCNNGC 3 cut(s) 103, 1501, 2139
CaiI CAGNNNCTG 3 cut(s) 776, 926, 2903
CciI TCATGA 1 cut(s) 1048
CfoI GCGC 1 cut(s) 2404
ClaI ATCGAT 1 cut(s) 1425
CseI GACGC 3 cut(s) 1173, 2609, 2687
CsiI ACCWGGT 1 cut(s) 1092
Csp6I GTAC 6 cut(s) 204, 768, 1491, 1889, 1990, 3565
CspCI CAANNNNNGTGG 2 cut(s) 2776, 2811
CviQI GTAC 6 cut(s) 204, 768, 1491, 1889, 1990, 3565
DdeI CTNAG 9 cut(s) 207, 445, 687, 727, 739, 1386, 1467, 1933, 2052
DraI TTTAAA 1 cut(s) 2526
EaeI YGGCCR 2 cut(s) 2566, 2833
Eam1104I CTCTTC 5 cut(s) 26, 112, 1560, 3207, 3366
EarI CTCTTC 5 cut(s) 26, 112, 1560, 3207, 3366
Ecl136II GAGCTC 2 cut(s) 105, 862
Eco130I CCWWGG 4 cut(s) 757, 2808, 3240, 3461
Eco24I GRGCYC 3 cut(s) 107, 864, 2541
Eco32I GATATC 1 cut(s) 3049
Eco47I GGWCC 4 cut(s) 278, 298, 1280, 1774
Eco53kI GAGCTC 2 cut(s) 105, 862
Eco88I CYCGRG 2 cut(s) 1662, 2205
Eco91I GGTNACC 1 cut(s) 34
EcoICRI GAGCTC 2 cut(s) 105, 862
EcoO109I RGGNCCY 3 cut(s) 440, 2468, 2538
EcoO65I GGTNACC 1 cut(s) 34
EcoRI GAATTC 1 cut(s) 2159
EcoRV GATATC 1 cut(s) 3049
EcoT14I CCWWGG 4 cut(s) 757, 2808, 3240, 3461
EcoT38I GRGCYC 3 cut(s) 107, 864, 2541
ErhI CCWWGG 4 cut(s) 757, 2808, 3240, 3461
FalI AAGNNNNNCTT 2 cut(s) 447, 479
FaqI GGGAC 1 cut(s) 738
FbaI TGATCA 2 cut(s) 114, 325
FblI GTMKAC 1 cut(s) 1402
Fnu4HI GCNGC 6 cut(s) 57, 337, 1377, 2382, 2772, 3286
FriOI GRGCYC 3 cut(s) 107, 864, 2541
Fsp4HI GCNGC 6 cut(s) 57, 337, 1377, 2382, 2772, 3286
FspBI CTAG 5 cut(s) 1475, 3168, 3180, 3270, 3282
FspI TGCGCA 1 cut(s) 2403
GlaI GCGC 1 cut(s) 2403
GluI GCNGC 6 cut(s) 57, 337, 1377, 2382, 2772, 3286
GsaI CCCAGC 2 cut(s) 1699, 2388
GsuI CTGGAG 3 cut(s) 1905, 2664, 2715
HapII CCGG 4 cut(s) 2757, 2832, 3265, 3401
HgaI GACGC 3 cut(s) 1173, 2609, 2687
HhaI GCGC 1 cut(s) 2404
Hin1I GRCGYC 2 cut(s) 1184, 2601
Hin6I GCGC 1 cut(s) 2402
HinP1I GCGC 1 cut(s) 2402
HincII GTYRAC 5 cut(s) 184, 637, 1000, 1732, 1909
HindII GTYRAC 5 cut(s) 184, 637, 1000, 1732, 1909
HindIII AAGCTT 4 cut(s) 1085, 1705, 2510, 2561
HpaII CCGG 4 cut(s) 2757, 2832, 3265, 3401
Hpy166II GTNNAC 9 cut(s) 184, 369, 637, 1000, 1151, 1403, 1732, 1909, 2068
Hpy8I GTNNAC 9 cut(s) 184, 369, 637, 1000, 1151, 1403, 1732, 1909, 2068
Hpy99I CGWCG 3 cut(s) 98, 1739, 2618
HpyCH4IV ACGT 5 cut(s) 559, 1620, 1737, 3363, 3563
HpyF10VI GCNNNNNNNGC 9 cut(s) 62, 342, 1265, 1376, 2619, 2654, 2704, 2804, 3256
HpyF3I CTNAG 9 cut(s) 207, 445, 687, 727, 739, 1386, 1467, 1933, 2052
HpySE526I ACGT 5 cut(s) 559, 1620, 1737, 3363, 3563
Hsp92I GRCGYC 2 cut(s) 1184, 2601
HspAI GCGC 1 cut(s) 2402
Ksp22I TGATCA 2 cut(s) 114, 325
LguI GCTCTTC 1 cut(s) 112
LmnI GCTCC 5 cut(s) 401, 1132, 2645, 2654, 2759
Lsp1109I GCAGC 5 cut(s) 323, 1388, 2368, 2783, 3297
LweI GCATC 8 cut(s) 127, 802, 943, 1792, 2027, 3017, 3151, 3259
MabI ACCWGGT 1 cut(s) 1092
MaeI CTAG 5 cut(s) 1475, 3168, 3180, 3270, 3282
MaeII ACGT 5 cut(s) 559, 1620, 1737, 3363, 3563
MflI RGATCY 1 cut(s) 2209
MhlI GDGCHC 5 cut(s) 107, 215, 427, 864, 2541
MlsI TGGCCA 1 cut(s) 2568
MluNI TGGCCA 1 cut(s) 2568
MlyI GAGTC 7 cut(s) 1267, 2771, 2900, 3011, 3197, 3391, 3438
MmeI TCCRAC 2 cut(s) 964, 1370
Mox20I TGGCCA 1 cut(s) 2568
MroXI GAANNNNTTC 1 cut(s) 3218
MscI TGGCCA 1 cut(s) 2568
MslI CAYNNNNRTG 7 cut(s) 729, 939, 1425, 1821, 2444, 2813, 3342
Msp20I TGGCCA 1 cut(s) 2568
MspA1I CMGCKG 1 cut(s) 2111
MspCI CTTAAG 1 cut(s) 2408
MspI CCGG 4 cut(s) 2757, 2832, 3265, 3401
Mva1269I GAATGC 2 cut(s) 2657, 3337
MvnI CGCG 2 cut(s) 32, 2793
MwoI GCNNNNNNNGC 9 cut(s) 62, 342, 1265, 1376, 2619, 2654, 2704, 2804, 3256
NciI CCSGG 1 cut(s) 2758
NcoI CCATGG 1 cut(s) 2808
NmuCI GTSAC 5 cut(s) 943, 967, 1060, 2899, 3170
NsbI TGCGCA 1 cut(s) 2403
NspI RCATGY 2 cut(s) 944, 3347
NspV TTCGAA 1 cut(s) 3212
PagI TCATGA 1 cut(s) 1048
PciI ACATGT 2 cut(s) 940, 3343
PciSI GCTCTTC 1 cut(s) 112
PctI GAATGC 2 cut(s) 2657, 3337
PdmI GAANNNNTTC 1 cut(s) 3218
PfeI GAWTC 7 cut(s) 68, 694, 975, 1667, 2873, 3299, 3510
PflMI CCANNNNNTGG 1 cut(s) 3506
PfoI TCCNGGA 3 cut(s) 541, 1882, 2680
PkrI GCNGC 6 cut(s) 58, 338, 1378, 2383, 2773, 3287
PleI GAGTC 7 cut(s) 1267, 2770, 2900, 3011, 3196, 3391, 3437
PpsI GAGTC 7 cut(s) 1267, 2770, 2900, 3011, 3196, 3391, 3437
Ppu21I YACGTR 1 cut(s) 560
PscI ACATGT 2 cut(s) 940, 3343
PshBI ATTAAT 1 cut(s) 357
Psp124BI GAGCTC 2 cut(s) 107, 864
PspEI GGTNACC 1 cut(s) 34
PspFI CCCAGC 2 cut(s) 1695, 2384
PspOMI GGGCCC 1 cut(s) 2537
PstNI CAGNNNCTG 3 cut(s) 776, 926, 2903
PsuI RGATCY 1 cut(s) 2209
PvuII CAGCTG 1 cut(s) 2111
RsaI GTAC 6 cut(s) 205, 769, 1492, 1890, 1991, 3566
RsaNI GTAC 6 cut(s) 204, 768, 1491, 1889, 1990, 3565
RseI CAYNNNNRTG 7 cut(s) 729, 939, 1425, 1821, 2444, 2813, 3342
SacI GAGCTC 2 cut(s) 107, 864
SapI GCTCTTC 1 cut(s) 112
SatI GCNGC 6 cut(s) 57, 337, 1377, 2382, 2772, 3286
ScaI AGTACT 1 cut(s) 205
SchI GAGTC 7 cut(s) 1267, 2771, 2900, 3011, 3197, 3391, 3438
SduI GDGCHC 5 cut(s) 107, 215, 427, 864, 2541
SexAI ACCWGGT 1 cut(s) 1092
SfaNI GCATC 8 cut(s) 127, 802, 943, 1792, 2027, 3017, 3151, 3259
SfcI CTRYAG 4 cut(s) 1188, 1494, 2577, 2623
SfuI TTCGAA 1 cut(s) 3212
SinI GGWCC 4 cut(s) 278, 298, 1280, 1774
SmiMI CAYNNNNRTG 7 cut(s) 729, 939, 1425, 1821, 2444, 2813, 3342
SmlI CTYRAG 1 cut(s) 2408
SmoI CTYRAG 1 cut(s) 2408
SpeI ACTAGT 1 cut(s) 1474
SsiI CCGC 7 cut(s) 32, 54, 57, 84, 1224, 1229, 1298
SspI AATATT 1 cut(s) 535
SspMI CTAG 5 cut(s) 1475, 3168, 3180, 3270, 3282
SstI GAGCTC 2 cut(s) 107, 864
StyI CCWWGG 4 cut(s) 757, 2808, 3240, 3461
TaiI ACGT 5 cut(s) 562, 1623, 1740, 3366, 3566
TaqI TCGA 9 cut(s) 96, 147, 1338, 1425, 2314, 2859, 3186, 3212, 3318
TatI WGTACW 2 cut(s) 203, 1888
TauI GCSGC 1 cut(s) 59
TfiI GAWTC 7 cut(s) 68, 694, 975, 1667, 2873, 3299, 3510
TseFI GTSAC 5 cut(s) 943, 967, 1060, 2899, 3170
TseI GCWGC 5 cut(s) 336, 1376, 2381, 2771, 3285
Tsp45I GTSAC 5 cut(s) 943, 967, 1060, 2899, 3170
TspGWI ACGGA 6 cut(s) 291, 370, 1017, 1338, 1434, 3181
Van91I CCANNNNNTGG 1 cut(s) 3506
Vha464I CTTAAG 1 cut(s) 2408
VpaK11BI GGWCC 4 cut(s) 278, 298, 1280, 1774
VspI ATTAAT 1 cut(s) 357
XceI RCATGY 2 cut(s) 944, 3347
XcmI CCANNNNNNNNNTGG 1 cut(s) 83
XmiI GTMKAC 1 cut(s) 1402
XmnI GAANNNNTTC 1 cut(s) 3218
XspI CTAG 5 cut(s) 1475, 3168, 3180, 3270, 3282
ZrmI AGTACT 1 cut(s) 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.