Rh3AG281600

Nodal modulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0011945
Physical Location & Seq
Reverse (-)
32857604 .. 32868873
11270 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG281600.1

Sequence Viewer

Length: 3600 bp
ATGGGCCTCAAACTCTTGCTCTTCGTCTTCGCCGTAACCTCACTGATCTCCACCGCCGCCTTCGCCGATTCAATCCACGGTTGCGGTGGCTTCGTCGAGGCGAGCTCTTCTTTGATCAAGGCCAGGAAGCCCTCCGATGCTAAATTCGACTATTCTCACATCACGGTTGAGCTTCGCACAGTTGACGGTTTGTTGAAGGAGAGTACTCAGTGCTCTCCCAATGGCTACTACTTCATCCCCGTCTATGACAAGGGTTCTTTTGTGATTAAAATCAACGGACCAGATGGATGGTCATTGGACCCTGATAAGGTTCCTGTTGTTGTTGATCACACTGGCTGCAATGGCAGTGAGGATATTAATTTTCGGTTTACTGGGTTCTCCGTGTCTGGTAGAGTTCTGGGAGCTGTTGGTGGAGAGAGCTGTGCCCTTCAAAATGGAGGTCCCTCAGACATCAAAGTTGAACTTTTGTCAGACAGTGGTGATGTTGTTTCTTCTGTTTCAACATCGTCAGGGGGAAGTTACTTATTCAAGAATATTATTCCAGGAAAATATGAAATACGTGCTTCACATCCGGACTTGAAAGTTGAAATAAGAGGTTCAACAGAGGTGAATGTGGGATTTGGAAACAGCGTGGTTGACGACATTTTCTTTGTTCCTGGCTATGACATAAGTGGATTTGTTGTTTCTCAGGGGAATCCGATATTGGGAGTTCATGTTTATTTACACTCAGATGATGTCTTAGAGGTAGATTGTCCCCAAGGTTCTGGTACAGGCTCTGAAATGAAGAAGGCTCTTTGCCACGCCGTATCTGATGCACAAGGAAAATTCATGTTCAAGTCGCTACCTTGTGGAACTTATGAGCTCATACCTTACTACAAGGGGGAAAACACAGTCTTTGATGTTTCACCTCCTGTCATGTCAGTAACTGTTGAGCATCAACATGTGACAGTTCTGCAAAAGTTCCAGGTCACTGGATTCTCTGTTGGAGGGCGTGTGGTTGACGGAAACGACATGGGGGTTGAAGGTGTTAAAATTATAGTTGATGGTCATGAAAGGTCTGTCACTGACAAACAAGGTTATTACAAGCTTGACCAGGTTACTTCCAATCGCTATACAATAGAAGCCACAAAGGAGCATTACAAGTTCAGTAAACTAACCGATTATTTGGTATTACCAAACATGGCGTCTATAGTGGCTATTAAAGCAGTTTCCTATGATGTCTGCGGTGCAGTGCAGATGGTTAGTGCTGGTTACAAGGCAAAGGTAGCTTTGACTCATGGACCTGAAAATGTCAAACCGCAAGTGAAACAGACCAATGGAAACGGAAATTTCTGCTTCGAGGTTCCAACTGGTGAATATCGTTTATCTGCTTTAGCAGCTTCACCTGAGAGTGCATCTGGTCTACTGTTTTTGCCATCTCATATCGATGTTGTTGTCAACAGTCCGTTATTGAACGTCAAATTTTCTCAGGCACTAGTTACAGTGCGTGGTACTGTAGCCTGCAAGGAGAAGTGTGGCACATCTGTTTCTGTTACCCTTAACAGTATAGGTGGTAAGCGTAATGAGGAGAGGAAAACAGTTAGTTTGACTGATGAGAGTAGTGAATTTGTCTTTCATAACGTCATCCCTGGAAAATACAGAGTTGAGGTTAAACGCAATTCCCAAGAATCCGTTAGTGGAGAAGATAATTGGTGCTGGGAAAAAAGCTTGATTGATGTGGATGTTGGTGTTGACGACGTAGAAGGAATTGAATTTGTCCAGAAAGGTTATTGGGTCCATGTCATTTCCACCCATGATGTAGATGCTTCTATGGTTCATCCAGATGGTTCATCCATGAAATTAAAAATTAAGAAAGGTTCCCAGAATATATGTGTAGAACATCCTGGAGTACATCAGCTTCACTTTGTCAACTCTTGTATTTTCTTTGGGAGCTCAGCAATTCAGATTGATACATCAAACCCATCGCCTATTCATCTAAAAGGAGAAAAGTACCTTCTTAAAGGACAAATCAATGTTGCATCAAGCTCATTTGATGGTGGGAACAAGTTATCTGAGAATTTCATTGTGGACATTCTGAATACTGGGGGAAGTATTATAGATAGTACAACAGCTGGACTTACTTCCACTGGGAATGAGCAAGCAAGTTATTCTGTGTATGAATTCTCTGTATGGGCCAATCTTGGAGAGAAACTTACCTTTGTTCCTCGGGATCCAAGAAATAATGAGATGGGGAAGATATTGTTTTATCCCAGACAACATCATGTCTTGGTTAAAAATGATGGTTGTCAAGCTATGATCCCTGCATTTAATGGTCGATTGGGTTTATATATTAAAGGATCAGTGTCTCCCCCTCTTTCTGATGTTCATATTAAGATTATTGCTGCTGGGAAAAGCCACATTGCGCAACTTAAGGAAGGGGAATTAGTTATTGAAACTACCACAGCTATGGATGGCTCGTTTGTTGGGGGGCCTTTATATGATGATATAACATACAATGTTGAGGCTTCAAAGCCTGGCTACCATTTAAAACAAGTTGGGCCCCATTCCTTTTCTTGTCAGAAGCTTGGCCAAATTTCTGTAGAAATATATTCTAAAGATGACGCCAAAGAGCCCATTCCTTCCGTGTTGTTATCTTTGAGTGGCGACGATGGCTACAGAAACAACTCAATATCTGGAGCTGGTGGAGCATTCCTCTTTAATAACTTATTTCCTGGAACTTTCTATTTGCGTCCTCTGCTAAAGGAGTTTGCCTTCTCGCCTCCAGCACAGGCTATTGATCTTGGCTCCGGGGAGTCTAAAGCAGCTATTTTCCAGGCCACTCGCGTTGCTTACAGCGCCATGGGCATGGTCGCTTTGTTGTCCGGCCAACCAATAGAAGGTGTCTTGATCGAGGCCAGATCAGAATCAAAAGGCTTCTACGAGGAAACAGTGACTGACTCATCTGGAAGTTATCGTTTGAGAGGACTTCTTCCTGACACAACCTATGTAATCAAAGTAGTAAAAAGGGATGGTTTGGGTAGCAGTAAAATTGAGAGGGCATCACCAGATTCGGTTCCAGTTAAGGTTGGGTATGAAGATATCAAAGGATTAGACTTTCTGGTGTTTGAACAGCCAGACACAACTATTTTAAGTTGCCATGTCGAAGGAATGAAGAATGAGGAACTCCACTCGCATCTACTGGTGGAAATCAAATCATCTAGTGACAATTCTAGGATCGAGTCTGTCTTCCCTCTTCCACTTTCGAACTTTTTCCAGGTGAAGGACTTACCCAAGGGTAAGCATCTTCTGCAACTCCGGTCTAGTCTTCCATCTAGCAGCCACAAATTTGAATCCGAGATTATTGAGGTCGATTTAGACAAGAATGCTCATATACATGTTGGCCCACTCAAATACGTTTATGAAGAGGATCACCAGAAGCAGGATTTGACTCCGGCACCGGTTTTTCCTCTTATCGTAGGAGTCTCGGTGATTGTTCTATTTCTTACTATCCCAAGGTTGAACGATTTGTATCAGTCCATGATTGGAACACCAACACCTGGATTCACTACAACTGCAAAGAAGGAAGTGAGGAAGCCCATGCTGAGAAAAAAGACGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CarboxypepD_reg

Protein Analysis

1199

Amino Acids

130.18

Weight (kDa)

5.87

Isoelectric Point (pI)

31.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NOMO1-like_1st PF22898 45 - 121 3.6e-31 BOS complex subunit NOMO1-like, first beta sandwich domain
NOMO1-like_2nd PF22904 123 - 216 1.1e-18 BOS complex subunit NOMO1-like, second beta sandwich domain
SdrD_B PF17210 147 - 192 6.9e-06 SdrD B-like domain
NOMO_3rd PF23193 261 - 324 9.9e-11 NOMO third transthyretin-like domain
NOMO1-like_2nd PF22904 324 - 384 2e-07 BOS complex subunit NOMO1-like, second beta sandwich domain
CarboxypepD_reg PF13620 327 - 386 1.2e-06 Carboxypeptidase regulatory-like domain
NOMO_5th PF23194 406 - 488 4.4e-10 NOMO fifth transthyretin-like domain
NOMO_6th PF23196 497 - 569 8.1e-07 NOMO sixth transthyretin-like domain
Ig_NOMO PF23141 591 - 663 2.6e-27 NOMO Ig-like domain
NOMO_8th PF23660 665 - 772 1.4e-41 NOMO eighth prealbumin-like domain
NOMO1-like_9th PF22902 774 - 846 6e-14 BOS complex subunit NOMO1-like, beta sandwich domain
CarboxypepD_reg PF13620 948 - 1006 3.6e-08 Carboxypeptidase regulatory-like domain
DUF7152 PF23662 1040 - 1138 1.4e-46 Domain of unknown function (DUF7152)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011945)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G62360 AT3G62360
fragaria_vesca FvH4_1g27060
malus_domestica MD09G1284300.v1.1 MD17G1277500.v1.1
prunus_persica Prupe.3G010500_v2.0.a1 Prupe.3G010500_v2.0.a1 Prupe.3G010500_v2.0.a1
pyrus_communis pycom17g27510
rosa_chinensis RchiOBHm_Chr3g0487381
rosa_laevigata RLG00000023009
rosa_multiflora Rmu_sc0001955.1_g000004
rosa_roxburghii Rroxscaffold_6G00394840
rosa_rugosa Rorug03G0233600
rosa_samantha Rh3AG281600 Rh3BG317200 Rh3CG315300 Rh3DG313200
rosa_wichuraiana Rw3G024930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2403
AccB1I GGYRCC 1 cut(s) 3433
AccB7I CCANNNNNTGG 1 cut(s) 3536
AccI GTMKAC 1 cut(s) 1402
AccII CGCG 1 cut(s) 2823
AccIII TCCGGA 1 cut(s) 571
AciI CCGC 5 cut(s) 54, 57, 84, 1224, 1298
AclWI GGATC 6 cut(s) 2204, 2217, 2290, 2344, 3221, 3414
AcoI YGGCCR 2 cut(s) 2566, 2863
AcyI GRCGYC 2 cut(s) 1184, 2601
AfaI GTAC 7 cut(s) 205, 769, 1492, 1890, 1991, 2104, 3596
AfiI CCNNNNNNNGG 6 cut(s) 307, 704, 2876, 3259, 3418, 3536
AflII CTTAAG 1 cut(s) 2408
AflIII ACRYGT 2 cut(s) 940, 3373
AgeI ACCGGT 1 cut(s) 3436
AhlI ACTAGT 1 cut(s) 1474
AjuI GAANNNNNNNTTGG 2 cut(s) 686, 718
Alw21I GWGCWC 4 cut(s) 107, 215, 864, 1934
Alw26I GTCTC 2 cut(s) 2349, 3466
AlwI GGATC 6 cut(s) 2204, 2217, 2290, 2344, 3221, 3414
AlwNI CAGNNNCTG 3 cut(s) 776, 926, 2933
Ama87I CYCGRG 1 cut(s) 2205
Aor13HI TCCGGA 1 cut(s) 571
ApaI GGGCCC 1 cut(s) 2541
ApeKI GCWGC 5 cut(s) 336, 1376, 2381, 2801, 3315
ArsI GACNNNNNNTTYG 6 cut(s) 424, 456, 632, 664, 1169, 1201
AseI ATTAAT 1 cut(s) 357
AsiGI ACCGGT 1 cut(s) 3436
Asp700I GAANNNNTTC 1 cut(s) 3248
AspLEI GCGC 2 cut(s) 2404, 2837
AsuC2I CCSGG 1 cut(s) 2788
AsuHPI GGTGA 9 cut(s) 491, 619, 897, 1364, 1374, 3033, 3268, 3401, 3478
AsuII TTCGAA 1 cut(s) 3242
AvaI CYCGRG 1 cut(s) 2205
AvaII GGWCC 5 cut(s) 278, 298, 440, 1280, 1774
BaeGI GKGCMC 2 cut(s) 427, 2541
BalI TGGCCA 1 cut(s) 2568
BamHI GGATCC 1 cut(s) 2209
BanI GGYRCC 1 cut(s) 3433
BanII GRGCYC 5 cut(s) 107, 864, 1934, 2541, 2613
BarI GAAGNNNNNNTAC 2 cut(s) 1881, 1913
BbsI GAAGAC 3 cut(s) 19, 3217, 3296
Bbv12I GWGCWC 4 cut(s) 107, 215, 864, 1934
BbvI GCAGC 5 cut(s) 323, 1388, 2368, 2813, 3327
BceAI ACGGC 2 cut(s) 17, 788
BclI TGATCA 2 cut(s) 114, 325
BcnI CCSGG 1 cut(s) 2788
BcoDI GTCTC 2 cut(s) 2349, 3466
BcuI ACTAGT 1 cut(s) 1474
BfaI CTAG 5 cut(s) 1475, 3198, 3210, 3300, 3312
BfmI CTRYAG 4 cut(s) 1188, 1494, 2577, 2653
BfoI RGCGCY 1 cut(s) 2838
BfrI CTTAAG 1 cut(s) 2408
BisI GCNGC 6 cut(s) 57, 337, 1377, 2382, 2802, 3316
BlpI GCTNAGC 1 cut(s) 1933
BlsI GCNGC 6 cut(s) 58, 338, 1378, 2383, 2803, 3317
BmcAI AGTACT 1 cut(s) 205
Bme18I GGWCC 5 cut(s) 278, 298, 440, 1280, 1774
BmeT110I CYCGRG 1 cut(s) 2205
BmrI ACTGGG 3 cut(s) 381, 2091, 2136
BmsI GCATC 9 cut(s) 127, 802, 943, 1403, 1792, 2027, 3047, 3181, 3289
BmuI ACTGGG 3 cut(s) 381, 2091, 2136
BpiI GAAGAC 3 cut(s) 19, 3217, 3296
BplI GAGNNNNNCTC 4 cut(s) 89, 121, 2676, 2708
BpmI CTGGAG 3 cut(s) 1905, 2694, 2745
Bpu1102I GCTNAGC 1 cut(s) 1933
Bpu14I TTCGAA 1 cut(s) 3242
BpuMI CCSGG 1 cut(s) 2788
Bsa29I ATCGAT 1 cut(s) 1425
BsaAI YACGTR 1 cut(s) 560
BsaBI GATNNNNATC 3 cut(s) 269, 2902, 3507
BsaHI GRCGYC 2 cut(s) 1184, 2601
BsaJI CCNNGG 8 cut(s) 76, 757, 1627, 2204, 2787, 2838, 3270, 3491
BsaWI WCCGGW 3 cut(s) 571, 3294, 3436
BsaXI ACNNNNNCTCC 6 cut(s) 699, 729, 978, 1008, 3450, 3480
Bsc4I CCNNNNNNNGG 6 cut(s) 307, 704, 2876, 3259, 3418, 3536
Bse118I RCCGGY 1 cut(s) 3436
Bse1I ACTGG 8 cut(s) 337, 376, 976, 1354, 2086, 2131, 3056, 3183
Bse3DI GCAATG 2 cut(s) 346, 2397
Bse8I GATNNNNATC 3 cut(s) 269, 2902, 3507
BseAI TCCGGA 1 cut(s) 571
BseCI ATCGAT 1 cut(s) 1425
BseDI CCNNGG 8 cut(s) 76, 757, 1627, 2204, 2787, 2838, 3270, 3491
BseJI GATNNNNATC 3 cut(s) 269, 2902, 3507
BseLI CCNNNNNNNGG 6 cut(s) 307, 704, 2876, 3259, 3418, 3536
BseMI GCAATG 2 cut(s) 346, 2397
BseMII CTCAG 9 cut(s) 221, 459, 701, 741, 1377, 1481, 1947, 2043, 3572
BseNI ACTGG 8 cut(s) 337, 376, 976, 1354, 2086, 2131, 3056, 3183
BseRI GAGGAG 1 cut(s) 1580
BseSI GKGCMC 2 cut(s) 427, 2541
BseXI GCAGC 5 cut(s) 323, 1388, 2368, 2813, 3327
BseYI CCCAGC 2 cut(s) 1695, 2384
BsgI GTGCAG 2 cut(s) 1248, 1253
Bsh1236I CGCG 1 cut(s) 2823
BshNI GGYRCC 1 cut(s) 3433
BshTI ACCGGT 1 cut(s) 3436
BshVI ATCGAT 1 cut(s) 1425
BsiHKAI GWGCWC 4 cut(s) 107, 215, 864, 1934
BsiHKCI CYCGRG 1 cut(s) 2205
BsiSI CCGG 6 cut(s) 572, 2787, 2862, 3295, 3431, 3437
BslFI GGGAC 2 cut(s) 426, 738
BslI CCNNNNNNNGG 6 cut(s) 307, 704, 2876, 3259, 3418, 3536
BsmAI GTCTC 2 cut(s) 2349, 3466
BsmFI GGGAC 2 cut(s) 426, 738
BsmI GAATGC 2 cut(s) 2687, 3367
BsoBI CYCGRG 1 cut(s) 2205
Bsp119I TTCGAA 1 cut(s) 3242
Bsp120I GGGCCC 1 cut(s) 2537
Bsp1286I GDGCHC 7 cut(s) 107, 215, 427, 864, 1934, 2541, 2613
Bsp13I TCCGGA 1 cut(s) 571
Bsp1720I GCTNAGC 1 cut(s) 1933
Bsp19I CCATGG 1 cut(s) 2838
BspACI CCGC 5 cut(s) 54, 57, 84, 1224, 1298
BspCNI CTCAG 9 cut(s) 220, 458, 700, 740, 1378, 1480, 1946, 2044, 3573
BspDI ATCGAT 1 cut(s) 1425
BspEI TCCGGA 1 cut(s) 571
BspFNI CGCG 1 cut(s) 2823
BspHI TCATGA 1 cut(s) 1048
BspPI GGATC 6 cut(s) 2204, 2217, 2290, 2344, 3221, 3414
BspQI GCTCTTC 2 cut(s) 26, 112
BspT104I TTCGAA 1 cut(s) 3242
BspT107I GGYRCC 1 cut(s) 3433
BspTI CTTAAG 1 cut(s) 2408
BsrDI GCAATG 2 cut(s) 346, 2397
BsrFI RCCGGY 1 cut(s) 3436
BsrI ACTGG 8 cut(s) 337, 376, 976, 1354, 2086, 2131, 3056, 3183
BssAI RCCGGY 1 cut(s) 3436
BssECI CCNNGG 8 cut(s) 76, 757, 1627, 2204, 2787, 2838, 3270, 3491
BssNI GRCGYC 2 cut(s) 1184, 2601
BssT1I CCWWGG 4 cut(s) 757, 2838, 3270, 3491
Bst6I CTCTTC 4 cut(s) 26, 112, 3237, 3396
BstACI GRCGYC 2 cut(s) 1184, 2601
BstAFI CTTAAG 1 cut(s) 2408
BstAPI GCANNNNNTGC 1 cut(s) 3286
BstBAI YACGTR 1 cut(s) 560
BstBI TTCGAA 1 cut(s) 3242
BstC8I GCNNGC 3 cut(s) 103, 1501, 2139
BstDSI CCRYGG 2 cut(s) 76, 2838
BstFNI CGCG 1 cut(s) 2823
BstH2I RGCGCY 1 cut(s) 2838
BstHHI GCGC 2 cut(s) 2404, 2837
BstMAI GTCTC 2 cut(s) 2349, 3466
BstNSI RCATGY 2 cut(s) 944, 3377
BstSFI CTRYAG 4 cut(s) 1188, 1494, 2577, 2653
BstSLI GKGCMC 2 cut(s) 427, 2541
BstUI CGCG 1 cut(s) 2823
BstV1I GCAGC 5 cut(s) 323, 1388, 2368, 2813, 3327
BstV2I GAAGAC 3 cut(s) 19, 3217, 3296
BstX2I RGATCY 1 cut(s) 2209
BstXI CCANNNNNNTGG 5 cut(s) 288, 764, 971, 2446, 2845
BstYI RGATCY 1 cut(s) 2209
Bsu15I ATCGAT 1 cut(s) 1425
BsuTUI ATCGAT 1 cut(s) 1425
BtgI CCRYGG 2 cut(s) 76, 2838
BtgZI GCGATG 1 cut(s) 1947
BtsI GCAGTG 2 cut(s) 352, 1236
Cac8I GCNNGC 3 cut(s) 103, 1501, 2139
CaiI CAGNNNCTG 3 cut(s) 776, 926, 2933
CciI TCATGA 1 cut(s) 1048
CfoI GCGC 2 cut(s) 2404, 2837
Cfr10I RCCGGY 1 cut(s) 3436
ClaI ATCGAT 1 cut(s) 1425
CseI GACGC 3 cut(s) 1173, 2609, 2717
CsiI ACCWGGT 1 cut(s) 1092
Csp6I GTAC 7 cut(s) 204, 768, 1491, 1889, 1990, 2103, 3595
CspAI ACCGGT 1 cut(s) 3436
CspCI CAANNNNNGTGG 2 cut(s) 2806, 2841
CviQI GTAC 7 cut(s) 204, 768, 1491, 1889, 1990, 2103, 3595
DraI TTTAAA 1 cut(s) 2526
EaeI YGGCCR 2 cut(s) 2566, 2863
Eam1104I CTCTTC 4 cut(s) 26, 112, 3237, 3396
EarI CTCTTC 4 cut(s) 26, 112, 3237, 3396
Ecl136II GAGCTC 3 cut(s) 105, 862, 1932
Eco130I CCWWGG 4 cut(s) 757, 2838, 3270, 3491
Eco24I GRGCYC 5 cut(s) 107, 864, 1934, 2541, 2613
Eco32I GATATC 1 cut(s) 3079
Eco47I GGWCC 5 cut(s) 278, 298, 440, 1280, 1774
Eco53kI GAGCTC 3 cut(s) 105, 862, 1932
Eco88I CYCGRG 1 cut(s) 2205
EcoICRI GAGCTC 3 cut(s) 105, 862, 1932
EcoO109I RGGNCCY 3 cut(s) 440, 2468, 2538
EcoRI GAATTC 1 cut(s) 2159
EcoRV GATATC 1 cut(s) 3079
EcoT14I CCWWGG 4 cut(s) 757, 2838, 3270, 3491
EcoT38I GRGCYC 5 cut(s) 107, 864, 1934, 2541, 2613
ErhI CCWWGG 4 cut(s) 757, 2838, 3270, 3491
FalI AAGNNNNNCTT 2 cut(s) 447, 479
FaqI GGGAC 2 cut(s) 426, 738
FbaI TGATCA 2 cut(s) 114, 325
FblI GTMKAC 1 cut(s) 1402
Fnu4HI GCNGC 6 cut(s) 57, 337, 1377, 2382, 2802, 3316
FriOI GRGCYC 5 cut(s) 107, 864, 1934, 2541, 2613
Fsp4HI GCNGC 6 cut(s) 57, 337, 1377, 2382, 2802, 3316
FspBI CTAG 5 cut(s) 1475, 3198, 3210, 3300, 3312
FspI TGCGCA 1 cut(s) 2403
GlaI GCGC 2 cut(s) 2403, 2836
GluI GCNGC 6 cut(s) 57, 337, 1377, 2382, 2802, 3316
GsaI CCCAGC 2 cut(s) 1699, 2388
GsuI CTGGAG 3 cut(s) 1905, 2694, 2745
HaeII RGCGCY 1 cut(s) 2838
HapII CCGG 6 cut(s) 572, 2787, 2862, 3295, 3431, 3437
HgaI GACGC 3 cut(s) 1173, 2609, 2717
HhaI GCGC 2 cut(s) 2404, 2837
Hin1I GRCGYC 2 cut(s) 1184, 2601
Hin6I GCGC 2 cut(s) 2402, 2835
HinP1I GCGC 2 cut(s) 2402, 2835
HincII GTYRAC 6 cut(s) 184, 637, 1000, 1438, 1732, 1909
HindII GTYRAC 6 cut(s) 184, 637, 1000, 1438, 1732, 1909
HindIII AAGCTT 3 cut(s) 1085, 1705, 2561
HpaII CCGG 6 cut(s) 572, 2787, 2862, 3295, 3431, 3437
HphI GGTGA 9 cut(s) 491, 619, 897, 1364, 1374, 3033, 3268, 3401, 3478
Hpy99I CGWCG 3 cut(s) 98, 1739, 2648
HpyCH4IV ACGT 6 cut(s) 559, 1455, 1620, 1737, 3393, 3593
HpySE526I ACGT 6 cut(s) 559, 1455, 1620, 1737, 3393, 3593
Hsp92I GRCGYC 2 cut(s) 1184, 2601
HspAI GCGC 2 cut(s) 2402, 2835
Kpn2I TCCGGA 1 cut(s) 571
Ksp22I TGATCA 2 cut(s) 114, 325
LguI GCTCTTC 2 cut(s) 26, 112
LmnI GCTCC 6 cut(s) 401, 1132, 1929, 2675, 2684, 2789
Lsp1109I GCAGC 5 cut(s) 323, 1388, 2368, 2813, 3327
LweI GCATC 9 cut(s) 127, 802, 943, 1403, 1792, 2027, 3047, 3181, 3289
MabI ACCWGGT 1 cut(s) 1092
MaeI CTAG 5 cut(s) 1475, 3198, 3210, 3300, 3312
MaeII ACGT 6 cut(s) 559, 1455, 1620, 1737, 3393, 3593
MflI RGATCY 1 cut(s) 2209
MhlI GDGCHC 7 cut(s) 107, 215, 427, 864, 1934, 2541, 2613
MlsI TGGCCA 1 cut(s) 2568
MluNI TGGCCA 1 cut(s) 2568
MlyI GAGTC 6 cut(s) 1267, 2801, 2930, 3227, 3421, 3468
MmeI TCCRAC 2 cut(s) 964, 1370
Mox20I TGGCCA 1 cut(s) 2568
MroI TCCGGA 1 cut(s) 571
MroXI GAANNNNTTC 1 cut(s) 3248
MscI TGGCCA 1 cut(s) 2568
MslI CAYNNNNRTG 7 cut(s) 729, 939, 1425, 1821, 2444, 2843, 3372
Msp20I TGGCCA 1 cut(s) 2568
MspA1I CMGCKG 1 cut(s) 2111
MspCI CTTAAG 1 cut(s) 2408
MspI CCGG 6 cut(s) 572, 2787, 2862, 3295, 3431, 3437
Mva1269I GAATGC 2 cut(s) 2687, 3367
MvnI CGCG 1 cut(s) 2823
NciI CCSGG 1 cut(s) 2788
NcoI CCATGG 1 cut(s) 2838
NmuCI GTSAC 5 cut(s) 943, 967, 1060, 2929, 3200
NsbI TGCGCA 1 cut(s) 2403
NspI RCATGY 2 cut(s) 944, 3377
NspV TTCGAA 1 cut(s) 3242
PagI TCATGA 1 cut(s) 1048
PciI ACATGT 2 cut(s) 940, 3373
PciSI GCTCTTC 2 cut(s) 26, 112
PcsI WCGNNNNNNNCGW 1 cut(s) 30
PctI GAATGC 2 cut(s) 2687, 3367
PdmI GAANNNNTTC 1 cut(s) 3248
PfeI GAWTC 8 cut(s) 68, 694, 975, 1667, 2903, 3047, 3329, 3540
PflMI CCANNNNNTGG 1 cut(s) 3536
PfoI TCCNGGA 3 cut(s) 541, 1882, 2710
PinAI ACCGGT 1 cut(s) 3436
PkrI GCNGC 6 cut(s) 58, 338, 1378, 2383, 2803, 3317
PleI GAGTC 6 cut(s) 1267, 2800, 2930, 3226, 3421, 3467
PpsI GAGTC 6 cut(s) 1267, 2800, 2930, 3226, 3421, 3467
Ppu21I YACGTR 1 cut(s) 560
PpuMI RGGWCCY 1 cut(s) 440
PscI ACATGT 2 cut(s) 940, 3373
PshBI ATTAAT 1 cut(s) 357
Psp124BI GAGCTC 3 cut(s) 107, 864, 1934
Psp5II RGGWCCY 1 cut(s) 440
PspFI CCCAGC 2 cut(s) 1695, 2384
PspOMI GGGCCC 1 cut(s) 2537
PspPPI RGGWCCY 1 cut(s) 440
PstNI CAGNNNCTG 3 cut(s) 776, 926, 2933
PsuI RGATCY 1 cut(s) 2209
PvuII CAGCTG 1 cut(s) 2111
RsaI GTAC 7 cut(s) 205, 769, 1492, 1890, 1991, 2104, 3596
RsaNI GTAC 7 cut(s) 204, 768, 1491, 1889, 1990, 2103, 3595
RseI CAYNNNNRTG 7 cut(s) 729, 939, 1425, 1821, 2444, 2843, 3372
SacI GAGCTC 3 cut(s) 107, 864, 1934
SapI GCTCTTC 2 cut(s) 26, 112
SatI GCNGC 6 cut(s) 57, 337, 1377, 2382, 2802, 3316
ScaI AGTACT 1 cut(s) 205
SchI GAGTC 6 cut(s) 1267, 2801, 2930, 3227, 3421, 3468
SduI GDGCHC 7 cut(s) 107, 215, 427, 864, 1934, 2541, 2613
SexAI ACCWGGT 1 cut(s) 1092
SfaNI GCATC 9 cut(s) 127, 802, 943, 1403, 1792, 2027, 3047, 3181, 3289
SfcI CTRYAG 4 cut(s) 1188, 1494, 2577, 2653
SfuI TTCGAA 1 cut(s) 3242
SinI GGWCC 5 cut(s) 278, 298, 440, 1280, 1774
SmiMI CAYNNNNRTG 7 cut(s) 729, 939, 1425, 1821, 2444, 2843, 3372
SmlI CTYRAG 1 cut(s) 2408
SmoI CTYRAG 1 cut(s) 2408
SpeI ACTAGT 1 cut(s) 1474
SsiI CCGC 5 cut(s) 54, 57, 84, 1224, 1298
SspI AATATT 1 cut(s) 535
SspMI CTAG 5 cut(s) 1475, 3198, 3210, 3300, 3312
SstI GAGCTC 3 cut(s) 107, 864, 1934
StyI CCWWGG 4 cut(s) 757, 2838, 3270, 3491
TaiI ACGT 6 cut(s) 562, 1458, 1623, 1740, 3396, 3596
TatI WGTACW 3 cut(s) 203, 1888, 2102
TauI GCSGC 1 cut(s) 59
TfiI GAWTC 8 cut(s) 68, 694, 975, 1667, 2903, 3047, 3329, 3540
TseFI GTSAC 5 cut(s) 943, 967, 1060, 2929, 3200
TseI GCWGC 5 cut(s) 336, 1376, 2381, 2801, 3315
Tsp45I GTSAC 5 cut(s) 943, 967, 1060, 2929, 3200
TspGWI ACGGA 7 cut(s) 291, 370, 1017, 1338, 1434, 1660, 2611
Van91I CCANNNNNTGG 1 cut(s) 3536
Vha464I CTTAAG 1 cut(s) 2408
VpaK11BI GGWCC 5 cut(s) 278, 298, 440, 1280, 1774
VspI ATTAAT 1 cut(s) 357
XceI RCATGY 2 cut(s) 944, 3377
XcmI CCANNNNNNNNNTGG 1 cut(s) 83
XmiI GTMKAC 1 cut(s) 1402
XmnI GAANNNNTTC 1 cut(s) 3248
XspI CTAG 5 cut(s) 1475, 3198, 3210, 3300, 3312
ZrmI AGTACT 1 cut(s) 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.