FvH4_1g29560

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0006095
Physical Location & Seq
Forward (+)
22734220 .. 22738354
4135 bp
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UTR
Exon/CDS
Intron
FvH4_1g29560.t3

Sequence Viewer

Length: 819 bp
ATGGCTACTAATGAAAATTTGCCACCAAATGTGATCAAGCAACTTGCGAAGGAATTGAAGAGTCTTGACGAATCCCCTCCTGAAGGCATTAAAGTTTGTGTGAATGATGATGACTTTTCACTCATATTTGCTGATATTGAAGGGCCAGCTGGCACTCCTTACGAGAATGGTGTTTTTCGTATGAAGTTGTTATTATCTCGTGACTTTCCACACTCTCCTCCCAAAGGTTACTTTCTGACCAGAATTTTCCATCCAAACATTGCAAATAACGGTGAGATTTGTGTGAACACATTAAAAAAAGATTGGAATCCAAGTCTTGGATTACGACATGTTCTCATCGTAGTCAGATGTCTGTTGATTGAACCATTTCCAGAATCAGCCTTAAATGAACAAGCAGGCAAGATGCTGCTTGAGAATTATGAGGAATATGCAAGACATGCCAGGCTTTATACTGGGATCCATGCCAAGCCAAAACCCAAATTTAAAACAGGAGCTATTTCTGAGTCTACTACTGCACTGAACGTCGACCAATCTAACACCTCGGCAGTTAATGCTGATCAAAAGATCACGGTAGGTGGTGCTGCTTTGCCACTGCCATCCCAAATCCCATCTATACTGGTCCCTTTGATTGTCACTGCAAGAGGACTTGGCCAGGATCAGCCGGCTATTGTAGCTTCTGCAGTGGAGACAAGAGTTGGTGGTACTGCTGCATCAACTGTAGCAAGTAGTACGACACTGAAGAAAGAAGCTGGCTTGACAAAAGCACAGGCAGAAAAGAAGAAAATTGATGCAAGGAAGAAAAGTCTGAAGAGATTATGA

Protein Analysis

273

Amino Acids

29.46

Weight (kDa)

9.12

Isoelectric Point (pI)

35.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 3 - 138 1.7e-38 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 317
AccI GTMKAC 2 cut(s) 506, 525
AclWI GGATC 3 cut(s) 451, 464, 663
AcoI YGGCCR 1 cut(s) 649
AcsI RAATTY 3 cut(s) 16, 243, 479
AcuI CTGAAG 2 cut(s) 102, 758
AfaI GTAC 2 cut(s) 703, 730
AfiI CCNNNNNNNGG 3 cut(s) 83, 224, 317
AflIII ACRYGT 1 cut(s) 328
AgsI TTSAA 3 cut(s) 58, 140, 362
AjnI CCWGG 2 cut(s) 440, 651
AluBI AGCT 4 cut(s) 149, 494, 674, 749
AluI AGCT 4 cut(s) 149, 494, 674, 749
Alw26I GTCTC 1 cut(s) 680
AlwI GGATC 3 cut(s) 451, 464, 663
AoxI GGCC 2 cut(s) 143, 649
ApeKI GCWGC 3 cut(s) 406, 581, 707
ApoI RAATTY 3 cut(s) 16, 243, 479
Asp700I GAANNNNTTC 1 cut(s) 366
AspS9I GGNCC 2 cut(s) 143, 619
AsuHPI GGTGA 1 cut(s) 284
AvaII GGWCC 1 cut(s) 619
BalI TGGCCA 1 cut(s) 651
BamHI GGATCC 1 cut(s) 456
BauI CACGAG 1 cut(s) 198
BbvI GCAGC 3 cut(s) 393, 568, 694
BccI CCATC 3 cut(s) 258, 604, 616
BciT130I CCWGG 2 cut(s) 442, 653
BclI TGATCA 2 cut(s) 33, 556
BcoDI GTCTC 1 cut(s) 680
BfmI CTRYAG 2 cut(s) 678, 717
BisI GCNGC 3 cut(s) 407, 582, 708
BlsI GCNGC 3 cut(s) 408, 583, 709
Bme1390I CCNGG 2 cut(s) 442, 653
Bme18I GGWCC 1 cut(s) 619
BmgT120I GGNCC 2 cut(s) 143, 619
BmiI GGNNCC 2 cut(s) 458, 621
BmrFI CCNGG 2 cut(s) 442, 653
BmrI ACTGGG 1 cut(s) 462
BmsI GCATC 3 cut(s) 393, 719, 778
BmuI ACTGGG 1 cut(s) 462
BpuEI CTTGAG 1 cut(s) 431
BsaBI GATNNNNATC 1 cut(s) 306
BsaJI CCNNGG 1 cut(s) 540
Bsc4I CCNNNNNNNGG 3 cut(s) 83, 224, 317
Bse118I RCCGGY 1 cut(s) 661
Bse1I ACTGG 2 cut(s) 457, 621
Bse3DI GCAATG 1 cut(s) 258
Bse8I GATNNNNATC 1 cut(s) 306
BseBI CCWGG 2 cut(s) 442, 653
BseDI CCNNGG 1 cut(s) 540
BseGI GGATG 2 cut(s) 250, 596
BseJI GATNNNNATC 1 cut(s) 306
BseLI CCNNNNNNNGG 3 cut(s) 83, 224, 317
BseMI GCAATG 1 cut(s) 258
BseMII CTCAG 1 cut(s) 492
BseNI ACTGG 2 cut(s) 457, 621
BseRI GAGGAG 1 cut(s) 207
BseXI GCAGC 3 cut(s) 393, 568, 694
BsgI GTGCAG 1 cut(s) 498
BshFI GGCC 2 cut(s) 145, 651
BsiSI CCGG 1 cut(s) 662
BslFI GGGAC 1 cut(s) 605
BslI CCNNNNNNNGG 3 cut(s) 83, 224, 317
BsmAI GTCTC 1 cut(s) 680
BsmFI GGGAC 1 cut(s) 605
BsnI GGCC 2 cut(s) 145, 651
Bsp143I GATC 5 cut(s) 33, 456, 556, 564, 655
BspANI GGCC 2 cut(s) 145, 651
BspCNI CTCAG 1 cut(s) 493
BspLI GGNNCC 2 cut(s) 458, 621
BspMAI CTGCAG 1 cut(s) 682
BspPI GGATC 3 cut(s) 451, 464, 663
BsrDI GCAATG 1 cut(s) 258
BsrFI RCCGGY 1 cut(s) 661
BsrI ACTGG 2 cut(s) 457, 621
BssAI RCCGGY 1 cut(s) 661
BssECI CCNNGG 1 cut(s) 540
BssMI GATC 5 cut(s) 33, 456, 556, 564, 655
BssSI CACGAG 1 cut(s) 198
Bst2BI CACGAG 1 cut(s) 198
Bst2UI CCWGG 2 cut(s) 442, 653
Bst4CI ACNGT 3 cut(s) 272, 571, 718
Bst6I CTCTTC 2 cut(s) 53, 803
BstAPI GCANNNNNTGC 2 cut(s) 437, 551
BstC8I GCNNGC 5 cut(s) 147, 151, 397, 663, 751
BstDEI CTNAG 1 cut(s) 501
BstENI CCTNNNNNAGG 2 cut(s) 81, 222
BstF5I GGATG 2 cut(s) 250, 596
BstKTI GATC 5 cut(s) 36, 459, 559, 567, 658
BstMAI GTCTC 1 cut(s) 680
BstMBI GATC 5 cut(s) 33, 456, 556, 564, 655
BstMWI GCNNNNNNNGC 3 cut(s) 437, 551, 671
BstNI CCWGG 2 cut(s) 442, 653
BstNSI RCATGY 2 cut(s) 332, 440
BstSCI CCNGG 2 cut(s) 440, 651
BstSFI CTRYAG 2 cut(s) 678, 717
BstV1I GCAGC 3 cut(s) 393, 568, 694
BstX2I RGATCY 1 cut(s) 456
BstYI RGATCY 1 cut(s) 456
BsuRI GGCC 2 cut(s) 145, 651
BtsCI GGATG 2 cut(s) 250, 596
BtsI GCAGTG 3 cut(s) 590, 633, 687
BtsIMutI CAGTG 5 cut(s) 515, 590, 633, 687, 734
Cac8I GCNNGC 5 cut(s) 147, 151, 397, 663, 751
Cfr10I RCCGGY 1 cut(s) 661
Cfr13I GGNCC 2 cut(s) 143, 619
Csp6I GTAC 2 cut(s) 702, 729
CviAII CATG 3 cut(s) 329, 437, 461
CviQI GTAC 2 cut(s) 702, 729
DdeI CTNAG 1 cut(s) 501
DpnI GATC 5 cut(s) 35, 458, 558, 566, 657
DpnII GATC 5 cut(s) 33, 456, 556, 564, 655
DraI TTTAAA 1 cut(s) 484
EaeI YGGCCR 1 cut(s) 649
Eam1104I CTCTTC 2 cut(s) 53, 803
EarI CTCTTC 2 cut(s) 53, 803
Eco47I GGWCC 1 cut(s) 619
Eco57I CTGAAG 2 cut(s) 102, 758
EcoNI CCTNNNNNAGG 2 cut(s) 81, 222
EcoRII CCWGG 2 cut(s) 440, 651
FaeI CATG 3 cut(s) 332, 440, 464
FaqI GGGAC 1 cut(s) 605
FatI CATG 3 cut(s) 328, 436, 460
FbaI TGATCA 2 cut(s) 33, 556
FblI GTMKAC 2 cut(s) 506, 525
Fnu4HI GCNGC 3 cut(s) 407, 582, 708
FokI GGATG 2 cut(s) 237, 583
Fsp4HI GCNGC 3 cut(s) 407, 582, 708
GluI GCNGC 3 cut(s) 407, 582, 708
HaeIII GGCC 2 cut(s) 145, 651
HapII CCGG 1 cut(s) 662
Hin1II CATG 3 cut(s) 332, 440, 464
HincII GTYRAC 1 cut(s) 526
HindII GTYRAC 1 cut(s) 526
HinfI GANTC 5 cut(s) 61, 71, 307, 374, 503
HpaII CCGG 1 cut(s) 662
HphI GGTGA 1 cut(s) 284
Hpy166II GTNNAC 3 cut(s) 286, 507, 526
Hpy188I TCNGA 4 cut(s) 237, 347, 502, 807
Hpy188III TCNNGA 4 cut(s) 65, 80, 200, 371
Hpy8I GTNNAC 3 cut(s) 286, 507, 526
Hpy99I CGWCG 1 cut(s) 527
HpyAV CCTTC 3 cut(s) 43, 77, 134
HpyCH4III ACNGT 3 cut(s) 272, 571, 718
HpyCH4IV ACGT 1 cut(s) 522
HpyCH4V TGCA 7 cut(s) 263, 431, 515, 638, 680, 710, 791
HpyF10VI GCNNNNNNNGC 3 cut(s) 437, 551, 671
HpyF3I CTNAG 1 cut(s) 501
HpySE526I ACGT 1 cut(s) 522
Hsp92II CATG 3 cut(s) 332, 440, 464
KroI GCCGGC 1 cut(s) 661
KroNI GCCGGC 1 cut(s) 663
Ksp22I TGATCA 2 cut(s) 33, 556
Kzo9I GATC 5 cut(s) 33, 456, 556, 564, 655
LmnI GCTCC 1 cut(s) 491
Lsp1109I GCAGC 3 cut(s) 393, 568, 694
LweI GCATC 3 cut(s) 393, 719, 778
MaeII ACGT 1 cut(s) 522
MaeIII GTNAC 3 cut(s) 200, 227, 631
MalI GATC 5 cut(s) 35, 458, 558, 566, 657
MboI GATC 5 cut(s) 33, 456, 556, 564, 655
MboII GAAGA 4 cut(s) 70, 751, 790, 808
MflI RGATCY 1 cut(s) 456
MlsI TGGCCA 1 cut(s) 651
MluCI AATT 6 cut(s) 16, 53, 243, 415, 479, 783
MluNI TGGCCA 1 cut(s) 651
MlyI GAGTC 2 cut(s) 70, 512
MnlI CCTC 5 cut(s) 87, 228, 415, 550, 635
Mox20I TGGCCA 1 cut(s) 651
MroNI GCCGGC 1 cut(s) 661
MroXI GAANNNNTTC 1 cut(s) 366
MscI TGGCCA 1 cut(s) 651
MseI TTAA 5 cut(s) 90, 293, 383, 483, 549
Msp20I TGGCCA 1 cut(s) 651
MspA1I CMGCKG 1 cut(s) 149
MspI CCGG 1 cut(s) 662
MspR9I CCNGG 2 cut(s) 442, 653
MvaI CCWGG 2 cut(s) 442, 653
MwoI GCNNNNNNNGC 3 cut(s) 437, 551, 671
NaeI GCCGGC 1 cut(s) 663
NdeII GATC 5 cut(s) 33, 456, 556, 564, 655
NgoMIV GCCGGC 1 cut(s) 661
NlaIII CATG 3 cut(s) 332, 440, 464
NlaIV GGNNCC 2 cut(s) 458, 621
NmeAIII GCCGAG 1 cut(s) 521
NmuCI GTSAC 2 cut(s) 200, 631
NspI RCATGY 2 cut(s) 332, 440
PciI ACATGT 1 cut(s) 328
PdiI GCCGGC 1 cut(s) 663
PdmI GAANNNNTTC 1 cut(s) 366
PfeI GAWTC 3 cut(s) 71, 307, 374
PflMI CCANNNNNTGG 1 cut(s) 317
PkrI GCNGC 3 cut(s) 408, 583, 709
PleI GAGTC 2 cut(s) 69, 511
PpsI GAGTC 2 cut(s) 69, 511
PscI ACATGT 1 cut(s) 328
Psp6I CCWGG 2 cut(s) 440, 651
PspGI CCWGG 2 cut(s) 440, 651
PspN4I GGNNCC 2 cut(s) 458, 621
PspPI GGNCC 2 cut(s) 143, 619
PstI CTGCAG 1 cut(s) 682
PsuI RGATCY 1 cut(s) 456
PvuII CAGCTG 1 cut(s) 149
RsaI GTAC 2 cut(s) 703, 730
RsaNI GTAC 2 cut(s) 702, 729
SalI GTCGAC 1 cut(s) 524
SaqAI TTAA 5 cut(s) 90, 293, 383, 483, 549
SatI GCNGC 3 cut(s) 407, 582, 708
Sau3AI GATC 5 cut(s) 33, 456, 556, 564, 655
Sau96I GGNCC 2 cut(s) 143, 619
SchI GAGTC 2 cut(s) 70, 512
ScrFI CCNGG 2 cut(s) 442, 653
SetI ASST 8 cut(s) 151, 229, 496, 525, 542, 577, 676, 751
SfaNI GCATC 3 cut(s) 393, 719, 778
SfcI CTRYAG 2 cut(s) 678, 717
SinI GGWCC 1 cut(s) 619
SmlI CTYRAG 1 cut(s) 410
SmoI CTYRAG 1 cut(s) 410
Sse9I AATT 6 cut(s) 16, 53, 243, 415, 479, 783
StyD4I CCNGG 2 cut(s) 440, 651
TaaI ACNGT 3 cut(s) 272, 571, 718
TaiI ACGT 1 cut(s) 525
TaqI TCGA 1 cut(s) 525
TasI AATT 6 cut(s) 16, 53, 243, 415, 479, 783
TfiI GAWTC 3 cut(s) 71, 307, 374
Tru1I TTAA 5 cut(s) 90, 293, 383, 483, 549
Tru9I TTAA 5 cut(s) 90, 293, 383, 483, 549
TscAI CASTG 5 cut(s) 522, 597, 640, 687, 741
TseFI GTSAC 2 cut(s) 200, 631
TseI GCWGC 3 cut(s) 406, 581, 707
Tsp45I GTSAC 2 cut(s) 200, 631
TspDTI ATGAA 3 cut(s) 27, 197, 402
TspRI CASTG 5 cut(s) 522, 597, 640, 687, 741
Van91I CCANNNNNTGG 1 cut(s) 317
VpaK11BI GGWCC 1 cut(s) 619
XagI CCTNNNNNAGG 2 cut(s) 81, 222
XapI RAATTY 3 cut(s) 16, 243, 479
XceI RCATGY 2 cut(s) 332, 440
XmiI GTMKAC 2 cut(s) 506, 525
XmnI GAANNNNTTC 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.