Rorug03G0270100

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0006095
Physical Location & Seq
Forward (+)
26612115 .. 26617303
5189 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0270100.1

Sequence Viewer

Length: 624 bp
ATGCGGAAGGTGGGCAATTTAGTACGGAAGGGATGCAAAGCTGTTCAGGATCTGAACTTGGTCAAGCTCCTCCAGTCTGAAATTCAACATGAGCTTTCCTCTAACCATTATCCGACAAGCAGAAGCAGTTCATTGGGAGATTTTGTGGTGGAATGGGATTCACCGCAGTCCAAAGATGTGGTTTTGAGGAGAAAATTGGAGTCTGGTGGAGAGGTTGCTGTTTCAGCTATGTTGGGTCCCTTTTCTGATACCACTGACAGAGAGGAGTATTTGTATCCAAGGGATGTTGTGATGAAGGTATGCGTGAAGAAGCCTGGGTTGAGCTCTATACTGCAGTTTGACTGTGAGATATTTGAGCAAGACGGTATTGGTTCTTCGTTTGCAATCTGGGAATTCTATTATATTCAATCACCAGCTCGAATTGGCCCTTCGGTCTATAGGGGTCCCTCATTCAGCTATGTGGATATCAAATTACATCTTGCACTCAAGGAATATCTAGTGGCCAAGGGTATTGGAGAAGAGCTTACTAACTTCCTCTTGCACCACCTACACAAAAAAGAACAAGGGCAATATGTGAACTGGCTACAGAAGCTTGAATCATTTGTTGCAAAACCAGAGTCATAG

Protein Analysis

207

Amino Acids

23.49

Weight (kDa)

6.6

Isoelectric Point (pI)

49.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAM33 PF02330 95 - 202 1.5e-16 Mitochondrial glycoprotein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 4, 164
AclWI GGATC 1 cut(s) 57
AcoI YGGCCR 1 cut(s) 501
AcsI RAATTY 2 cut(s) 81, 392
AfaI GTAC 1 cut(s) 24
AgsI TTSAA 3 cut(s) 86, 407, 596
AjnI CCWGG 1 cut(s) 313
AluBI AGCT 9 cut(s) 41, 67, 94, 227, 324, 416, 456, 523, 592
AluI AGCT 9 cut(s) 41, 67, 94, 227, 324, 416, 456, 523, 592
Alw21I GWGCWC 1 cut(s) 326
AlwI GGATC 1 cut(s) 57
AlwNI CAGNNNCTG 1 cut(s) 52
AoxI GGCC 2 cut(s) 424, 501
ApoI RAATTY 2 cut(s) 81, 392
ArsI GACNNNNNNTTYG 1 cut(s) 602
Asp700I GAANNNNTTC 1 cut(s) 127
AspS9I GGNCC 3 cut(s) 236, 425, 443
AsuHPI GGTGA 2 cut(s) 153, 402
AvaII GGWCC 2 cut(s) 236, 443
BalI TGGCCA 1 cut(s) 503
BanII GRGCYC 1 cut(s) 326
BarI GAAGNNNNNNTAC 2 cut(s) 358, 390
Bbv12I GWGCWC 1 cut(s) 326
BciT130I CCWGG 1 cut(s) 315
BciVI GTATCC 1 cut(s) 285
BfaI CTAG 1 cut(s) 497
BfmI CTRYAG 3 cut(s) 332, 436, 584
BfuI GTATCC 1 cut(s) 285
Bme1390I CCNGG 1 cut(s) 315
Bme18I GGWCC 2 cut(s) 236, 443
BmgT120I GGNCC 3 cut(s) 236, 425, 443
BmiI GGNNCC 4 cut(s) 237, 238, 444, 445
BmrFI CCNGG 1 cut(s) 315
BmsI GCATC 1 cut(s) 23
BplI GAGNNNNNCTC 2 cut(s) 83, 115
BpmI CTGGAG 1 cut(s) 56
BpuEI CTTGAG 1 cut(s) 470
BsaJI CCNNGG 3 cut(s) 278, 314, 504
BsaXI ACNNNNNCTCC 4 cut(s) 129, 159, 257, 287
Bse1I ACTGG 2 cut(s) 73, 584
BseBI CCWGG 1 cut(s) 315
BseDI CCNNGG 3 cut(s) 278, 314, 504
BseGI GGATG 2 cut(s) 38, 289
BseNI ACTGG 2 cut(s) 73, 584
BseRI GAGGAG 3 cut(s) 59, 202, 278
BshFI GGCC 2 cut(s) 426, 503
BsiHKAI GWGCWC 1 cut(s) 326
BslFI GGGAC 2 cut(s) 222, 429
BsmFI GGGAC 2 cut(s) 222, 429
BsnI GGCC 2 cut(s) 426, 503
Bsp1286I GDGCHC 1 cut(s) 326
Bsp143I GATC 1 cut(s) 49
BspACI CCGC 2 cut(s) 4, 164
BspANI GGCC 2 cut(s) 426, 503
BspLI GGNNCC 4 cut(s) 237, 238, 444, 445
BspMAI CTGCAG 1 cut(s) 336
BspPI GGATC 1 cut(s) 57
BspQI GCTCTTC 1 cut(s) 513
BsrI ACTGG 2 cut(s) 73, 584
BssECI CCNNGG 3 cut(s) 278, 314, 504
BssMI GATC 1 cut(s) 49
BssT1I CCWWGG 2 cut(s) 278, 504
Bst2UI CCWGG 1 cut(s) 315
Bst4CI ACNGT 2 cut(s) 344, 365
Bst6I CTCTTC 1 cut(s) 513
BstF5I GGATG 2 cut(s) 38, 289
BstKTI GATC 1 cut(s) 52
BstMBI GATC 1 cut(s) 49
BstMWI GCNNNNNNNGC 2 cut(s) 224, 589
BstNI CCWGG 1 cut(s) 315
BstSCI CCNGG 1 cut(s) 313
BstSFI CTRYAG 3 cut(s) 332, 436, 584
BstX2I RGATCY 1 cut(s) 49
BstXI CCANNNNNNTGG 1 cut(s) 178
BstYI RGATCY 1 cut(s) 49
BsuI GTATCC 1 cut(s) 285
BsuRI GGCC 2 cut(s) 426, 503
BtsCI GGATG 2 cut(s) 38, 289
BtsIMutI CAGTG 1 cut(s) 252
CaiI CAGNNNCTG 1 cut(s) 52
Cfr13I GGNCC 3 cut(s) 236, 425, 443
Csp6I GTAC 1 cut(s) 23
CviAII CATG 1 cut(s) 89
CviQI GTAC 1 cut(s) 23
DpnI GATC 1 cut(s) 51
DpnII GATC 1 cut(s) 49
EaeI YGGCCR 1 cut(s) 501
Eam1104I CTCTTC 1 cut(s) 513
EarI CTCTTC 1 cut(s) 513
Ecl136II GAGCTC 1 cut(s) 324
Eco130I CCWWGG 2 cut(s) 278, 504
Eco24I GRGCYC 1 cut(s) 326
Eco32I GATATC 1 cut(s) 466
Eco47I GGWCC 2 cut(s) 236, 443
Eco53kI GAGCTC 1 cut(s) 324
EcoICRI GAGCTC 1 cut(s) 324
EcoO109I RGGNCCY 2 cut(s) 236, 443
EcoRI GAATTC 1 cut(s) 392
EcoRII CCWGG 1 cut(s) 313
EcoRV GATATC 1 cut(s) 466
EcoT14I CCWWGG 2 cut(s) 278, 504
EcoT38I GRGCYC 1 cut(s) 326
ErhI CCWWGG 2 cut(s) 278, 504
FaeI CATG 1 cut(s) 92
FaiI YATR 9 cut(s) 90, 230, 301, 329, 402, 438, 459, 573, 622
FaqI GGGAC 2 cut(s) 222, 429
FatI CATG 1 cut(s) 88
FokI GGATG 2 cut(s) 45, 296
FriOI GRGCYC 1 cut(s) 326
FspBI CTAG 1 cut(s) 497
GsuI CTGGAG 1 cut(s) 56
HaeIII GGCC 2 cut(s) 426, 503
Hin1II CATG 1 cut(s) 92
HindIII AAGCTT 1 cut(s) 590
HinfI GANTC 4 cut(s) 158, 200, 596, 617
HphI GGTGA 2 cut(s) 153, 402
Hpy166II GTNNAC 1 cut(s) 577
Hpy188I TCNGA 4 cut(s) 54, 79, 114, 247
Hpy188III TCNNGA 1 cut(s) 47
Hpy8I GTNNAC 1 cut(s) 577
HpyAV CCTTC 3 cut(s) 22, 289, 438
HpyCH4III ACNGT 2 cut(s) 344, 365
HpyCH4V TGCA 6 cut(s) 36, 334, 383, 482, 541, 608
HpyF10VI GCNNNNNNNGC 2 cut(s) 224, 589
Hsp92II CATG 1 cut(s) 92
KflI GGGWCCC 2 cut(s) 236, 443
Kzo9I GATC 1 cut(s) 49
LguI GCTCTTC 1 cut(s) 513
LmnI GCTCC 1 cut(s) 72
LpnPI CCDG 8 cut(s) 32, 86, 189, 300, 327, 373, 426, 565
LweI GCATC 1 cut(s) 23
MaeI CTAG 1 cut(s) 497
MalI GATC 1 cut(s) 51
MboI GATC 1 cut(s) 49
MboII GAAGA 3 cut(s) 319, 366, 530
MflI RGATCY 1 cut(s) 49
MhlI GDGCHC 1 cut(s) 326
MlsI TGGCCA 1 cut(s) 503
MluCI AATT 6 cut(s) 16, 81, 194, 392, 420, 470
MluNI TGGCCA 1 cut(s) 503
MlyI GAGTC 1 cut(s) 209
MmeI TCCRAC 1 cut(s) 137
MnlI CCTC 7 cut(s) 80, 109, 180, 205, 256, 457, 545
Mox20I TGGCCA 1 cut(s) 503
MroXI GAANNNNTTC 1 cut(s) 127
MscI TGGCCA 1 cut(s) 503
Msp20I TGGCCA 1 cut(s) 503
MspR9I CCNGG 1 cut(s) 315
MvaI CCWGG 1 cut(s) 315
MwoI GCNNNNNNNGC 2 cut(s) 224, 589
NdeII GATC 1 cut(s) 49
NlaIII CATG 1 cut(s) 92
NlaIV GGNNCC 4 cut(s) 237, 238, 444, 445
PciSI GCTCTTC 1 cut(s) 513
PdmI GAANNNNTTC 1 cut(s) 127
PfeI GAWTC 2 cut(s) 158, 596
PleI GAGTC 1 cut(s) 208
PpsI GAGTC 1 cut(s) 208
PpuMI RGGWCCY 2 cut(s) 236, 443
Psp124BI GAGCTC 1 cut(s) 326
Psp5II RGGWCCY 2 cut(s) 236, 443
Psp6I CCWGG 1 cut(s) 313
PspGI CCWGG 1 cut(s) 313
PspN4I GGNNCC 4 cut(s) 237, 238, 444, 445
PspPI GGNCC 3 cut(s) 236, 425, 443
PspPPI RGGWCCY 2 cut(s) 236, 443
PstI CTGCAG 1 cut(s) 336
PstNI CAGNNNCTG 1 cut(s) 52
PsuI RGATCY 1 cut(s) 49
RsaI GTAC 1 cut(s) 24
RsaNI GTAC 1 cut(s) 23
SacI GAGCTC 1 cut(s) 326
SapI GCTCTTC 1 cut(s) 513
Sau3AI GATC 1 cut(s) 49
Sau96I GGNCC 3 cut(s) 236, 425, 443
SchI GAGTC 1 cut(s) 209
ScrFI CCNGG 1 cut(s) 315
SduI GDGCHC 1 cut(s) 326
SfaNI GCATC 1 cut(s) 23
SfcI CTRYAG 3 cut(s) 332, 436, 584
SinI GGWCC 2 cut(s) 236, 443
SmlI CTYRAG 1 cut(s) 485
SmoI CTYRAG 1 cut(s) 485
Sse9I AATT 6 cut(s) 16, 81, 194, 392, 420, 470
SsiI CCGC 2 cut(s) 4, 164
SspMI CTAG 1 cut(s) 497
SstI GAGCTC 1 cut(s) 326
StyD4I CCNGG 1 cut(s) 313
StyI CCWWGG 2 cut(s) 278, 504
TaaI ACNGT 2 cut(s) 344, 365
TaqI TCGA 1 cut(s) 418
TaqII GACCGA 1 cut(s) 421
TasI AATT 6 cut(s) 16, 81, 194, 392, 420, 470
TfiI GAWTC 2 cut(s) 158, 596
TscAI CASTG 1 cut(s) 259
TspDTI ATGAA 2 cut(s) 120, 308
TspGWI ACGGA 1 cut(s) 40
TspRI CASTG 1 cut(s) 259
VpaK11BI GGWCC 2 cut(s) 236, 443
XapI RAATTY 2 cut(s) 81, 392
XmnI GAANNNNTTC 1 cut(s) 127
XspI CTAG 1 cut(s) 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.