FvH4_1g30070

Belongs to the complex I 75 kDa subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0011004
Physical Location & Seq
Forward (+)
23815140 .. 23820365
5226 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g30070.t1

Sequence Viewer

Length: 2193 bp
ATGGGGTTAGGATCGCTTGCTTCCAGGGCCCTACGCCCCAGAGCCGCAAGGCACCTCCTCCACAGGACCATGGTCACCTCCACCCCCGAGCCATCCACCGCCGCCCAGCCCGAGCTCCCTCCCCGTACCCCCGTCGGCGGCGCCCGAGTCCACTTCGCCAACCCCGACGATGCGATCGAGGTTTTCGTGGACGGATACCCCGTCAAAATCCCCAAGGGCATGTCTGTCTTGCAGGCCTGTGAGGTCGCCGGCGTTGACATTCCTAGGTTTTGCTACCACAGCCGGTTGTCCATCGCCGGTAACTGCCGTATGTGTCTCGTCGAGGTTGAGAAGTCTCCCAAGCCCGTCGCCTCTTGCGCCATGCCTGCCATGCCTGGTATGAAAATTAAGACCGACACTCCAATTGCCAAGAAGGCTCGCGAAGGAGTGATGGAGTTTTTGTTGATGAATCATCCTTTGGACTGTCCAATTTGTGATCAGGGTGGAGAATGTGATCTCCAGGATCAGTCTATGGCCTTTGGATCTGACCGTGGCCGATTCACTGAAATGAAGAGATCAGTAGTGGACAAGAATCTTGGTCCTTTGGTCAAGACTGTCATGACCCGCTGTATTCAATGTACAAGGTGCGTCAGGTTTGCAACAGAGGTTGCTGGGGTTCAGGACCTTGGAATGTTAGGCCGTGGAAGTGGAGAGGAAATTGGGACTTATGTTGAAAAGCTTATGACCAGTGAACTTTCGGGAAATGTTATAGATATCTGTCCTGTGGGAGCCCTAACCTCAAAACCATTTGCATTTAAAGCTCGTAATTGGGAGTTAAAGGGAACAGAGACCATTGATGTTACTGATGCTGTTGGATCCAATATTCGAATTGATAGTAGAGGTCCAGAAGTCATGCGCATTGTTCCACGTTTAAATGAGGACATAAATGAGGAATGGATATCAGACAAGACTCGATTTTGTTATGACGGTTTGAAGAGGCAAAGATTGAATGACCCTATGATTCGTAGTGCTGATGGACGCTTTAAGCCCGTGAGCTGGCGTGATGCCCTTGCTGTTGTTGCTGAGATGGCTCATAAAGTTAAGCCAGAAGAAATTGTTGGGGTTGCTGGTCAGCTGTCTGATGCCGAGTCCATGATGGCACTAAAAGACTTCTTAAATAAAATGGGGTCTAACAATGTGTGGTGCGAAGGAAATGGTTCCAACCGTGATGCGGATCTTCGATCTGGATATATAATGAACAGTAGCATTGCTGGACTTGAAAAGGCGGATGCTTTTCTATTGGTTGGCACCCAGCCAAGAGTAGAAGCTGCTATTGTAAATGCTAGAATCCGGAAGACAGTGTTATCTAACCAAGCCAAGGTTGGGTACGTTGGTCCTGAAGCTGATTTGAATTATGAACACCAGCATCTTGGAACAGGCCCCGATACACTTCTAGAACTTGCTGAGCGTCGCCACCCCTTTTCCTCGGTCCTCTTAAATGCCAAGAACCCTGCCATTATTGTCGGTGCTGGGCTGTTTGAGAGAAAAGACAAGGATGCAATTTTCTCAGCTCTTGAAACCATTGCCAAATATGCGAAGGCTGTAAGGCCTGATTGGAATGGATTTAATGTACTGCTTCTCAAAGCGGCCCAGGCTGCAGCACTTGACCTTGGACTTGTGCCGGAATCTGAAAACAGCATTGAGTCTGCAAAATTTGTTTATCTGATGGGTGCTGATGATGTTAGCTTAGACAGGGTTCCATCTGATGCGTTTGTGGTTTATCAAGGGCATCATGGTGACCGAGGTGTGTATCGTGCTAATGTCATCCTCCCTGCAGCAGCTTTCAGTGAAAAGGAAGGAACATATGTAAATACTGAAGGGTGCTCTCAGCAAACAATGCCTGCAGTTCCAACTGTCGGTGATGCCAGGGATGACTGGAAGATACTCCGCGCTCTATCTGAGGTAGCAGGCGTGAAGCTGCCTTATGATTCAGTTGGGGCCATCCGATCCCGGATAAAGACTGTGGCACCAAACATCTTAAATGTAGACGAGAGAGAACCAGCTACATTTTCATTTTCGATTAAGCCCGAGACCACTCAGAAAATGGATTCGACTCCATTTGGTACTGCTGTTGAGAATTTCTATATGACTGATTCCATCACCAGGGCATCAAAGATAATGGCACAATGCAGTGCATTGTTGTTGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko04723 ko04932 ko05010 ko05012 ko05016 map00190 map01100 map04714 map04723 map04932 map05010 map05012 map05016
Pfam Domains
Protein Families
Fer2_4 Molybdopterin NADH-G_4Fe-4S_3 NADH_dhqG_C

Protein Analysis

731

Amino Acids

79.57

Weight (kDa)

6.64

Isoelectric Point (pI)

35.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer2_4 PF13510 57 - 133 6.1e-19 2Fe-2S iron-sulfur cluster binding domain
Fer2 PF00111 62 - 111 7.5e-07 2Fe-2S iron-sulfur cluster binding domain
NADH-G_4Fe-4S_3 PF10588 140 - 177 1.4e-16 NADH-ubiquinone oxidoreductase-G iron-sulfur binding region
Fer4_Nqo3 PF22117 193 - 259 4.7e-33 NADH-quinone oxidoreductase subunit 3, ferredoxin-like domain
Fer4_NDSU1 PF22151 272 - 322 5.5e-30 NADH-ubiquinone oxidoreductase NDSU1/NuoG-like, 4Fe-4S domain
Molybdopterin PF00384 328 - 648 5.4e-109 Molybdopterin oxidoreductase
NADH_dhqG_C PF09326 688 - 723 8.5e-13 NADH-ubiquinone oxidoreductase subunit G, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011004)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37510 AT5G37510
fragaria_vesca FvH4_1g30070
malus_domestica MD09G1268900.v1.1 MD17G1267300.v1.1
prunus_persica Prupe.3G165400_v2.0.a1
pyrus_communis pycom09g18080 pycom17g26640
rosa_chinensis RchiOBHm_Chr3g0491991
rosa_laevigata RLG00000022799 RLG00000022807
rosa_multiflora Rmu_sc0005238.1_g000005 Rmu_sc0036946.1_g000001
rosa_roxburghii Rroxscaffold_6G00392660
rosa_rugosa Rorug03G0251000
rosa_samantha Rh3AG301000 Rh3BG337200 Rh3CG334300 Rh3DG336600
rosa_wichuraiana Rw3G026610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 896
AccB1I GGYRCC 4 cut(s) 51, 140, 1286, 2005
AccI GTMKAC 1 cut(s) 2025
AccII CGCG 2 cut(s) 420, 1929
AccIII TCCGGA 1 cut(s) 1329
AciI CCGC 9 cut(s) 45, 99, 102, 138, 604, 1211, 1265, 1625, 1927
AclWI GGATC 7 cut(s) 19, 510, 529, 849, 862, 1221, 1980
AcoI YGGCCR 1 cut(s) 532
AcsI RAATTY 2 cut(s) 1691, 2116
AcuI CTGAAG 2 cut(s) 1398, 1875
AcyI GRCGYC 1 cut(s) 141
AfaI GTAC 5 cut(s) 127, 619, 1367, 1611, 2104
AfiI CCNNNNNNNGG 7 cut(s) 137, 1035, 1210, 1357, 1362, 1895, 2142
AgsI TTSAA 8 cut(s) 614, 713, 973, 988, 1259, 1390, 1556, 2185
AjnI CCWGG 6 cut(s) 23, 373, 498, 1629, 1904, 2141
AjuI GAANNNNNNNTTGG 4 cut(s) 440, 472, 1080, 1112
Alw21I GWGCWC 2 cut(s) 117, 1865
Alw26I GTCTC 4 cut(s) 320, 339, 821, 2063
AlwI GGATC 7 cut(s) 19, 510, 529, 849, 862, 1221, 1980
Ama87I CYCGRG 4 cut(s) 86, 110, 144, 2066
Aor13HI TCCGGA 1 cut(s) 1329
AoxI GGCC 9 cut(s) 27, 234, 513, 532, 676, 1417, 1586, 1626, 1977
ApaI GGGCCC 1 cut(s) 31
ApeKI GCWGC 6 cut(s) 1307, 1634, 1637, 1814, 1817, 1957
ApoI RAATTY 2 cut(s) 1691, 2116
Asp700I GAANNNNTTC 1 cut(s) 1195
AspA2I CCTAGG 1 cut(s) 263
AspLEI GCGC 4 cut(s) 143, 359, 897, 1931
AsuC2I CCSGG 1 cut(s) 1990
AsuHPI GGTGA 4 cut(s) 67, 1787, 1910, 2131
AsuII TTCGAA 1 cut(s) 865
AvaI CYCGRG 4 cut(s) 86, 110, 144, 2066
AvaII GGWCC 6 cut(s) 66, 578, 661, 881, 1373, 1468
AvrII CCTAGG 1 cut(s) 263
BaeGI GKGCMC 1 cut(s) 31
BaeI ACNNNNGTAYC 4 cut(s) 1357, 1390, 2094, 2127
BamHI GGATCC 1 cut(s) 854
BanI GGYRCC 4 cut(s) 51, 140, 1286, 2005
BanII GRGCYC 3 cut(s) 31, 117, 772
BbsI GAAGAC 1 cut(s) 1340
Bbv12I GWGCWC 2 cut(s) 117, 1865
BbvI GCAGC 6 cut(s) 1294, 1621, 1649, 1826, 1829, 1944
BceAI ACGGC 2 cut(s) 291, 663
BciT130I CCWGG 6 cut(s) 25, 375, 500, 1631, 1906, 2143
BciVI GTATCC 1 cut(s) 188
BclI TGATCA 1 cut(s) 475
BcnI CCSGG 1 cut(s) 1990
BcoDI GTCTC 4 cut(s) 320, 339, 821, 2063
BfaI CTAG 3 cut(s) 264, 1323, 1433
BfmI CTRYAG 3 cut(s) 1635, 1812, 1881
BfoI RGCGCY 1 cut(s) 144
BfuI GTATCC 1 cut(s) 188
BglI GCCNNNNNGGC 1 cut(s) 413
BlnI CCTAGG 1 cut(s) 263
BlpI GCTNAGC 1 cut(s) 1443
Bme1390I CCNGG 7 cut(s) 25, 375, 500, 1631, 1906, 1990, 2143
Bme18I GGWCC 6 cut(s) 66, 578, 661, 881, 1373, 1468
BmeT110I CYCGRG 4 cut(s) 86, 110, 144, 2066
BmrFI CCNGG 7 cut(s) 25, 375, 500, 1631, 1906, 1990, 2143
BoxI GACNNNNGTC 1 cut(s) 71
BpiI GAAGAC 1 cut(s) 1340
BpmI CTGGAG 1 cut(s) 482
Bpu1102I GCTNAGC 1 cut(s) 1443
Bpu14I TTCGAA 1 cut(s) 865
BpuMI CCSGG 1 cut(s) 1990
BsaBI GATNNNNATC 1 cut(s) 1212
BsaHI GRCGYC 1 cut(s) 141
BsaI GGTCTC 2 cut(s) 821, 2063
BsaWI WCCGGW 1 cut(s) 1329
BsaXI ACNNNNNCTCC 4 cut(s) 382, 412, 425, 455
Bsc4I CCNNNNNNNGG 7 cut(s) 137, 1035, 1210, 1357, 1362, 1895, 2142
Bse118I RCCGGY 3 cut(s) 248, 282, 296
Bse1I ACTGG 2 cut(s) 726, 1919
Bse3DI GCAATG 2 cut(s) 1245, 1560
Bse8I GATNNNNATC 1 cut(s) 1212
BseAI TCCGGA 1 cut(s) 1329
BseBI CCWGG 6 cut(s) 25, 375, 500, 1631, 1906, 2143
BseGI GGATG 7 cut(s) 92, 451, 1273, 1540, 1803, 1915, 1980
BseJI GATNNNNATC 1 cut(s) 1212
BseLI CCNNNNNNNGG 7 cut(s) 137, 1035, 1210, 1357, 1362, 1895, 2142
BseMI GCAATG 2 cut(s) 1245, 1560
BseMII CTCAG 6 cut(s) 1053, 1434, 1560, 1880, 1929, 2090
BseNI ACTGG 2 cut(s) 726, 1919
BseRI GAGGAG 1 cut(s) 47
BseSI GKGCMC 1 cut(s) 31
BseXI GCAGC 6 cut(s) 1294, 1621, 1649, 1826, 1829, 1944
BseYI CCCAGC 4 cut(s) 105, 650, 1290, 1508
Bsh1236I CGCG 2 cut(s) 420, 1929
Bsh1285I CGRYCG 1 cut(s) 177
BshFI GGCC 9 cut(s) 29, 236, 515, 534, 678, 1419, 1588, 1628, 1979
BshNI GGYRCC 4 cut(s) 51, 140, 1286, 2005
BsiEI CGRYCG 1 cut(s) 177
BsiHKAI GWGCWC 2 cut(s) 117, 1865
BsiHKCI CYCGRG 4 cut(s) 86, 110, 144, 2066
BsiSI CCGG 6 cut(s) 249, 283, 297, 1330, 1661, 1990
BslFI GGGAC 1 cut(s) 715
BslI CCNNNNNNNGG 7 cut(s) 137, 1035, 1210, 1357, 1362, 1895, 2142
BsmAI GTCTC 4 cut(s) 320, 339, 821, 2063
BsmFI GGGAC 1 cut(s) 715
BsnI GGCC 9 cut(s) 29, 236, 515, 534, 678, 1419, 1588, 1628, 1979
Bso31I GGTCTC 2 cut(s) 821, 2063
BsoBI CYCGRG 4 cut(s) 86, 110, 144, 2066
Bsp119I TTCGAA 1 cut(s) 865
Bsp120I GGGCCC 1 cut(s) 27
Bsp1286I GDGCHC 4 cut(s) 31, 117, 772, 1865
Bsp13I TCCGGA 1 cut(s) 1329
Bsp1407I TGTACA 1 cut(s) 617
Bsp1720I GCTNAGC 1 cut(s) 1443
Bsp19I CCATGG 1 cut(s) 69
Bsp68I TCGCGA 1 cut(s) 420
BspACI CCGC 9 cut(s) 45, 99, 102, 138, 604, 1211, 1265, 1625, 1927
BspANI GGCC 9 cut(s) 29, 236, 515, 534, 678, 1419, 1588, 1628, 1979
BspCNI CTCAG 6 cut(s) 1054, 1435, 1559, 1879, 1930, 2089
BspEI TCCGGA 1 cut(s) 1329
BspFNI CGCG 2 cut(s) 420, 1929
BspHI TCATGA 1 cut(s) 597
BspMAI CTGCAG 3 cut(s) 1639, 1816, 1885
BspPI GGATC 7 cut(s) 19, 510, 529, 849, 862, 1221, 1980
BspT104I TTCGAA 1 cut(s) 865
BspT107I GGYRCC 4 cut(s) 51, 140, 1286, 2005
BspTNI GGTCTC 2 cut(s) 821, 2063
BsrDI GCAATG 2 cut(s) 1245, 1560
BsrFI RCCGGY 3 cut(s) 248, 282, 296
BsrGI TGTACA 1 cut(s) 617
BsrI ACTGG 2 cut(s) 726, 1919
BssAI RCCGGY 3 cut(s) 248, 282, 296
BssNI GRCGYC 1 cut(s) 141
BssT1I CCWWGG 6 cut(s) 69, 213, 263, 664, 1356, 1648
Bst2UI CCWGG 6 cut(s) 25, 375, 500, 1631, 1906, 2143
Bst4CI ACNGT 9 cut(s) 464, 530, 595, 968, 1205, 1241, 1339, 1894, 2002
Bst6I CTCTTC 2 cut(s) 545, 968
BstACI GRCGYC 1 cut(s) 141
BstAPI GCANNNNNTGC 1 cut(s) 1876
BstAUI TGTACA 1 cut(s) 617
BstBI TTCGAA 1 cut(s) 865
BstC8I GCNNGC 8 cut(s) 18, 234, 250, 366, 418, 1037, 1881, 1948
BstDEI CTNAG 7 cut(s) 1062, 1443, 1546, 1726, 1866, 1938, 2076
BstDSI CCRYGG 3 cut(s) 69, 529, 679
BstEII GGTNACC 2 cut(s) 73, 1775
BstF5I GGATG 7 cut(s) 92, 451, 1273, 1540, 1803, 1915, 1980
BstFNI CGCG 2 cut(s) 420, 1929
BstH2I RGCGCY 1 cut(s) 144
BstHHI GCGC 4 cut(s) 143, 359, 897, 1931
BstMAI GTCTC 4 cut(s) 320, 339, 821, 2063
BstMCI CGRYCG 1 cut(s) 177
BstNI CCWGG 6 cut(s) 25, 375, 500, 1631, 1906, 2143
BstNSI RCATGY 1 cut(s) 223
BstPAI GACNNNNGTC 1 cut(s) 71
BstPI GGTNACC 2 cut(s) 73, 1775
BstSCI CCNGG 7 cut(s) 23, 373, 498, 1629, 1904, 1988, 2141
BstSFI CTRYAG 3 cut(s) 1635, 1812, 1881
BstSLI GKGCMC 1 cut(s) 31
BstUI CGCG 2 cut(s) 420, 1929
BstV1I GCAGC 6 cut(s) 1294, 1621, 1649, 1826, 1829, 1944
BstV2I GAAGAC 1 cut(s) 1340
BstX2I RGATCY 3 cut(s) 521, 854, 1213
BstXI CCANNNNNNTGG 1 cut(s) 1409
BstYI RGATCY 3 cut(s) 521, 854, 1213
BsuI GTATCC 1 cut(s) 188
BsuRI GGCC 9 cut(s) 29, 236, 515, 534, 678, 1419, 1588, 1628, 1979
BtgI CCRYGG 3 cut(s) 69, 529, 679
BtgZI GCGATG 1 cut(s) 277
BtsCI GGATG 7 cut(s) 92, 451, 1273, 1540, 1803, 1915, 1980
BtsI GCAGTG 1 cut(s) 2176
BtsIMutI CAGTG 5 cut(s) 540, 733, 1344, 1831, 2176
BtuMI TCGCGA 1 cut(s) 420
Cac8I GCNNGC 8 cut(s) 18, 234, 250, 366, 418, 1037, 1881, 1948
CciI TCATGA 1 cut(s) 597
CfoI GCGC 4 cut(s) 143, 359, 897, 1931
Cfr10I RCCGGY 3 cut(s) 248, 282, 296
CseI GACGC 3 cut(s) 616, 1026, 1436
Csp6I GTAC 5 cut(s) 126, 618, 1366, 1610, 2103
CviAII CATG 8 cut(s) 70, 220, 361, 370, 598, 892, 1132, 1772
CviQI GTAC 5 cut(s) 126, 618, 1366, 1610, 2103
DdeI CTNAG 7 cut(s) 1062, 1443, 1546, 1726, 1866, 1938, 2076
DinI GGCGCC 1 cut(s) 142
DraI TTTAAA 2 cut(s) 796, 912
EaeI YGGCCR 1 cut(s) 532
Eam1104I CTCTTC 2 cut(s) 545, 968
EarI CTCTTC 2 cut(s) 545, 968
EciI GGCGGA 1 cut(s) 1280
Ecl136II GAGCTC 1 cut(s) 115
Eco130I CCWWGG 6 cut(s) 69, 213, 263, 664, 1356, 1648
Eco147I AGGCCT 2 cut(s) 236, 1588
Eco24I GRGCYC 3 cut(s) 31, 117, 772
Eco31I GGTCTC 2 cut(s) 821, 2063
Eco32I GATATC 2 cut(s) 754, 939
Eco47I GGWCC 6 cut(s) 66, 578, 661, 881, 1373, 1468
Eco53kI GAGCTC 1 cut(s) 115
Eco57I CTGAAG 2 cut(s) 1398, 1875
Eco88I CYCGRG 4 cut(s) 86, 110, 144, 2066
Eco91I GGTNACC 2 cut(s) 73, 1775
EcoICRI GAGCTC 1 cut(s) 115
EcoO109I RGGNCCY 4 cut(s) 27, 28, 661, 1418
EcoO65I GGTNACC 2 cut(s) 73, 1775
EcoRII CCWGG 6 cut(s) 23, 373, 498, 1629, 1904, 2141
EcoRV GATATC 2 cut(s) 754, 939
EcoT14I CCWWGG 6 cut(s) 69, 213, 263, 664, 1356, 1648
EcoT38I GRGCYC 3 cut(s) 31, 117, 772
EgeI GGCGCC 1 cut(s) 142
EheI GGCGCC 1 cut(s) 142
ErhI CCWWGG 6 cut(s) 69, 213, 263, 664, 1356, 1648
FaeI CATG 8 cut(s) 73, 223, 364, 373, 601, 895, 1135, 1775
FaqI GGGAC 1 cut(s) 715
FatI CATG 8 cut(s) 69, 219, 360, 369, 597, 891, 1131, 1771
FauI CCCGC 1 cut(s) 611
FauNDI CATATG 1 cut(s) 1843
FbaI TGATCA 1 cut(s) 475
FblI GTMKAC 1 cut(s) 2025
FokI GGATG 7 cut(s) 79, 438, 1280, 1547, 1790, 1922, 1967
FriOI GRGCYC 3 cut(s) 31, 117, 772
FspAI RTGCGCAY 1 cut(s) 896
FspBI CTAG 3 cut(s) 264, 1323, 1433
FspI TGCGCA 1 cut(s) 896
GlaI GCGC 4 cut(s) 142, 358, 896, 1930
GsaI CCCAGC 4 cut(s) 109, 654, 1294, 1512
GsuI CTGGAG 1 cut(s) 482
HaeII RGCGCY 1 cut(s) 144
HaeIII GGCC 9 cut(s) 29, 236, 515, 534, 678, 1419, 1588, 1628, 1979
HapII CCGG 6 cut(s) 249, 283, 297, 1330, 1661, 1990
HgaI GACGC 3 cut(s) 616, 1026, 1436
HhaI GCGC 4 cut(s) 143, 359, 897, 1931
Hin1I GRCGYC 1 cut(s) 141
Hin1II CATG 8 cut(s) 73, 223, 364, 373, 601, 895, 1135, 1775
Hin6I GCGC 4 cut(s) 141, 357, 895, 1929
HinP1I GCGC 4 cut(s) 141, 357, 895, 1929
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HindIII AAGCTT 1 cut(s) 716
HpaII CCGG 6 cut(s) 249, 283, 297, 1330, 1661, 1990
HphI GGTGA 4 cut(s) 67, 1787, 1910, 2131
Hpy166II GTNNAC 6 cut(s) 151, 190, 256, 565, 731, 2026
Hpy188I TCNGA 9 cut(s) 526, 943, 1120, 1669, 1704, 1744, 1939, 1985, 2079
Hpy8I GTNNAC 6 cut(s) 151, 190, 256, 565, 731, 2026
Hpy99I CGWCG 5 cut(s) 137, 170, 323, 350, 1452
HpyAV CCTTC 6 cut(s) 406, 416, 1181, 1570, 1829, 1850
HpyCH4III ACNGT 9 cut(s) 464, 530, 595, 968, 1205, 1241, 1339, 1894, 2002
HpyCH4IV ACGT 2 cut(s) 907, 1368
HpyF3I CTNAG 7 cut(s) 1062, 1443, 1546, 1726, 1866, 1938, 2076
HpySE526I ACGT 2 cut(s) 907, 1368
Hsp92I GRCGYC 1 cut(s) 141
Hsp92II CATG 8 cut(s) 73, 223, 364, 373, 601, 895, 1135, 1775
HspAI GCGC 4 cut(s) 141, 357, 895, 1929
KasI GGCGCC 1 cut(s) 140
Kpn2I TCCGGA 1 cut(s) 1329
KroI GCCGGC 1 cut(s) 248
KroNI GCCGGC 1 cut(s) 250
Ksp22I TGATCA 1 cut(s) 475
LmnI GCTCC 2 cut(s) 120, 767
Lsp1109I GCAGC 6 cut(s) 1294, 1621, 1649, 1826, 1829, 1944
MaeI CTAG 3 cut(s) 264, 1323, 1433
MaeII ACGT 2 cut(s) 907, 1368
MaeIII GTNAC 4 cut(s) 73, 299, 838, 1775
MboII GAAGA 6 cut(s) 562, 985, 1100, 1208, 1345, 1930
MfeI CAATTG 1 cut(s) 402
MflI RGATCY 3 cut(s) 521, 854, 1213
MhlI GDGCHC 4 cut(s) 31, 117, 772, 1865
Mly113I GGCGCC 1 cut(s) 141
MlyI GAGTC 5 cut(s) 156, 943, 1136, 1691, 2086
MmeI TCCRAC 3 cut(s) 832, 1224, 1913
MreI CGCCGGCG 1 cut(s) 248
MroI TCCGGA 1 cut(s) 1329
MroNI GCCGGC 1 cut(s) 248
MroXI GAANNNNTTC 1 cut(s) 1195
MslI CAYNNNNRTG 2 cut(s) 545, 1773
MspA1I CMGCKG 2 cut(s) 606, 1114
MspI CCGG 6 cut(s) 249, 283, 297, 1330, 1661, 1990
MspR9I CCNGG 7 cut(s) 25, 375, 500, 1631, 1906, 1990, 2143
MunI CAATTG 1 cut(s) 402
MvaI CCWGG 6 cut(s) 25, 375, 500, 1631, 1906, 2143
MvnI CGCG 2 cut(s) 420, 1929
NaeI GCCGGC 1 cut(s) 250
NarI GGCGCC 1 cut(s) 141
NciI CCSGG 1 cut(s) 1990
NcoI CCATGG 1 cut(s) 69
NdeI CATATG 1 cut(s) 1843
NgoMIV GCCGGC 1 cut(s) 248
NlaIII CATG 8 cut(s) 73, 223, 364, 373, 601, 895, 1135, 1775
NmeAIII GCCGAG 1 cut(s) 1150
NmuCI GTSAC 2 cut(s) 73, 1775
NruI TCGCGA 1 cut(s) 420
NsbI TGCGCA 1 cut(s) 896
NspI RCATGY 1 cut(s) 223
NspV TTCGAA 1 cut(s) 865
PagI TCATGA 1 cut(s) 597
PceI AGGCCT 2 cut(s) 236, 1588
PcsI WCGNNNNNNNCGW 4 cut(s) 162, 174, 183, 198
PdiI GCCGGC 1 cut(s) 250
PdmI GAANNNNTTC 1 cut(s) 1195
PfeI GAWTC 9 cut(s) 448, 537, 571, 1000, 1326, 1664, 1967, 2087, 2132
PfoI TCCNGGA 2 cut(s) 498, 1988
Ple19I CGATCG 1 cut(s) 177
PleI GAGTC 5 cut(s) 155, 943, 1135, 1690, 2086
PluTI GGCGCC 1 cut(s) 144
PpsI GAGTC 5 cut(s) 155, 943, 1135, 1690, 2086
PpuMI RGGWCCY 1 cut(s) 661
PshAI GACNNNNGTC 1 cut(s) 71
Psp124BI GAGCTC 1 cut(s) 117
Psp5II RGGWCCY 1 cut(s) 661
Psp6I CCWGG 6 cut(s) 23, 373, 498, 1629, 1904, 2141
PspEI GGTNACC 2 cut(s) 73, 1775
PspFI CCCAGC 4 cut(s) 105, 650, 1290, 1508
PspGI CCWGG 6 cut(s) 23, 373, 498, 1629, 1904, 2141
PspOMI GGGCCC 1 cut(s) 27
PspPPI RGGWCCY 1 cut(s) 661
PstI CTGCAG 3 cut(s) 1639, 1816, 1885
PsuI RGATCY 3 cut(s) 521, 854, 1213
PvuI CGATCG 1 cut(s) 177
PvuII CAGCTG 1 cut(s) 1114
RruI TCGCGA 1 cut(s) 420
RsaI GTAC 5 cut(s) 127, 619, 1367, 1611, 2104
RsaNI GTAC 5 cut(s) 126, 618, 1366, 1610, 2103
RseI CAYNNNNRTG 2 cut(s) 545, 1773
SacI GAGCTC 1 cut(s) 117
SchI GAGTC 5 cut(s) 156, 943, 1136, 1691, 2086
ScrFI CCNGG 7 cut(s) 25, 375, 500, 1631, 1906, 1990, 2143
SduI GDGCHC 4 cut(s) 31, 117, 772, 1865
SfcI CTRYAG 3 cut(s) 1635, 1812, 1881
SfoI GGCGCC 1 cut(s) 142
SfuI TTCGAA 1 cut(s) 865
SgrAI CRCCGGYG 1 cut(s) 248
SinI GGWCC 6 cut(s) 66, 578, 661, 881, 1373, 1468
SmiMI CAYNNNNRTG 2 cut(s) 545, 1773
SseBI AGGCCT 2 cut(s) 236, 1588
SsiI CCGC 9 cut(s) 45, 99, 102, 138, 604, 1211, 1265, 1625, 1927
SspDI GGCGCC 1 cut(s) 140
SspI AATATT 1 cut(s) 862
SspMI CTAG 3 cut(s) 264, 1323, 1433
SstI GAGCTC 1 cut(s) 117
StuI AGGCCT 2 cut(s) 236, 1588
StyD4I CCNGG 7 cut(s) 23, 373, 498, 1629, 1904, 1988, 2141
StyI CCWWGG 6 cut(s) 69, 213, 263, 664, 1356, 1648
TaaI ACNGT 9 cut(s) 464, 530, 595, 968, 1205, 1241, 1339, 1894, 2002
TaiI ACGT 2 cut(s) 910, 1371
TaqI TCGA 7 cut(s) 177, 321, 865, 952, 1219, 2057, 2090
TaqII GACCGA 3 cut(s) 407, 1456, 1794
TatI WGTACW 2 cut(s) 617, 1609
TauI GCSGC 4 cut(s) 47, 104, 141, 1628
TfiI GAWTC 9 cut(s) 448, 537, 571, 1000, 1326, 1664, 1967, 2087, 2132
TscAI CASTG 5 cut(s) 547, 733, 1344, 1831, 2176
TseFI GTSAC 2 cut(s) 73, 1775
TseI GCWGC 6 cut(s) 1307, 1634, 1637, 1814, 1817, 1957
Tsp45I GTSAC 2 cut(s) 73, 1775
TspDTI ATGAA 6 cut(s) 395, 461, 563, 1250, 1410, 2040
TspGWI ACGGA 1 cut(s) 207
TspRI CASTG 5 cut(s) 547, 733, 1344, 1831, 2176
VpaK11BI GGWCC 6 cut(s) 66, 578, 661, 881, 1373, 1468
XapI RAATTY 2 cut(s) 1691, 2116
XbaI TCTAGA 1 cut(s) 1432
XceI RCATGY 1 cut(s) 223
XcmI CCANNNNNNNNNTGG 2 cut(s) 1358, 2080
XmaJI CCTAGG 1 cut(s) 263
XmiI GTMKAC 1 cut(s) 2025
XmnI GAANNNNTTC 1 cut(s) 1195
XspI CTAG 3 cut(s) 264, 1323, 1433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.