MD17G1267300.v1.1

Belongs to the complex I 75 kDa subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0011004
Physical Location & Seq
Forward (+)
32748049 .. 32752862
4814 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1267300.v1.1.491

Sequence Viewer

Length: 2250 bp
ATGGGGTTGGGATCGCTTGCTTCGAGGGCCATGAGGCCCACAGCATCAAGGCTCCTCAGCTCCCAGAACCCTAGACCCTTCTTCTCCTTCCACAGAGCGGTCTCAACCACGCCGGAGCTCCAGAACGCCGATCAATCAGCGTCGGCGGCCCAGCCGGACCCCCCCGCAGATCTCCCTCCTCGGACCCCTGTGGGCGGGGCCCGTGTCCACTTCCCGAATCCCGAAGATGCCATCGAAGTCTTCGTCGATGGATACCCCGTCAAAATCCCCAAGGGATTTACTGTATTGCAGGCCTGTGAGGTCGCCGGCGTTGATATCCCTAGATTCTGCTACCACAGCCGGTTGTCGATCGCCGGTAATTGCCGTATGTGCCTTGTTGAGGTTGAGAAGTCGCCGAAGCCCGTCGCCTCCTGCGCCATGCCTGCCATGCCTGGCATGAAAATTAAGACTGATACTCCAATAGCAAAGAAGGCTCGAGAAGGGGTGATGGAGTTTTTGCTGATGAATCATCCCTTGGATTGTCCAATTTGCGATCAGGGAGGAGAATGTGATCTGCAGGATCAGTCTATGGCATTTGGATCTGACCGCGGCCGTTTCACTGAGGTTAAGAGATCCGTAGTTGATAAGAATCTTGGACCTTTGGTGAAGACTGTAATGACTCGGTGTATTCAATGTACAAGGTGTGTCAGATTTGCAACAGAGGTTGCTGGGGTTCAGGACCTTGGCATGTTAGGTCGTGGCAGCGGAGAGGAAATTGGGACTTATGTCGAAAAACTTATGACAAGTGAGCTTTCTGGAAATGTGATAGATATCTGTCCTGTGGGAGCCCTAACCTCAAAACCGTTTGCATTTAAAGCCCGAAATTGGGAATTAAAGGGAACAGAGACCATTGATATTACTGATGCCGTTGGATCCAACATTCGAATTGATAGCCGAGGTCCAGAGGTCTTGCGCATTGTCCCACGATTAAATGAGGACATAAATGAAGAATGGATATCAGACAAGACTCGATTCTGTTATGATGGTTTGAAGAGGCAGAGGTTAAATGACCCTATGATTCGTGGTGCTGATGGGCGCTTTAAGCCTGTGAGCTGGCGTGATGCACTTGCTGTAGTTGCCGAGATGGCTCATCAAGTTAAACCAGAGGAGATCGTTGGGGTTGCTGGTCAGCTATCTGATGCTGAGTCTATGATGGCACTGAAAGACTTCTTAAATAAAATGGGGTCAAACAATGTCTGGTGTGAAGCAAATGGTTCGAACTGTGATGCTGATCTTCGATCCGGATATATTATGAATAGTACCATTGCTGGTCTTGAGAAAGCAGATGCTTTTCTGTTGGTTGGTACCCAGCCAAGGGTAGAAGCTGCTATTGTAAATGCTAGAATCCGGAAGACGGTGCAAGCTAATCACGCCAAGGTTGGGTACGTTGGCCCTCCAGCTGATTTCAACTACGATCATGAGCATCTCGGCACAGGCCCTCAGACACTTCTAGAAATTGCTGAACGTCGTCACCCATTTTCCTCGGTCCTCACAAATGCTAAAAACCCTGCCATCGTTGTTGGTGCTGGGGTCTTTGAGAGAAAAGACAAGGATGCAATTTTTTCTGCTGTTGAAGCCATGGGAAAATACGCTAATGTTGTAAGGCCTGATTGGAATGGATTCAATGTACTGCTTCTCAAAGCCGCCCAGGCTGCAGCACTTGACCTTGGGCTTGTGCCAGAATCTGAAAACAGCATCGAGTCTGCAAAGTTTGTTTACCTGATGGGCGCTGATGATACCAACTTGGAGAAGGTTCCAAGTGATGCATTTGTGGTTTATCAAGGGCATCATGGAGACCGAGGTGTGTATCGTGCTAACGTCATCCTTCCTGCAGCAGCATTCAGTGAAAAGGAAGGAACATACGTAAACACTGAAGGGTGCTCACAGCAAACAGTACCTGCAGTTCCGACTGTCGGTGATGCCAGGGATGACTGGAAGATAATTCGGGCTCTATCTGAGGTGGCAGGCGTGCGGCTGCCATATGATACGCTTGGGGCAATCCGATCCCGGATCAGGACAGTGGCACCAAACATTTTGCAAGTAGACGAGAGAGAGCCAGCTACCTTTTCATTATCGATTAAGCCCAAATCCACTCAGAAAGTGGATACCACTCCATTTGGGACTGCAGTTGAGAATTTCTACATGACCGATTCGATCACCAGGGCTTCGAAGATAATGGCACAATGCAGTTCGTTGCTCCTGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko04723 ko04932 ko05010 ko05012 ko05016 map00190 map01100 map04714 map04723 map04932 map05010 map05012 map05016
Pfam Domains
Protein Families
Fer2_4 Molybdopterin NADH-G_4Fe-4S_3 NADH_dhqG_C

Protein Analysis

750

Amino Acids

81.5

Weight (kDa)

6.33

Isoelectric Point (pI)

35.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer2_4 PF13510 76 - 152 2.3e-19 2Fe-2S iron-sulfur cluster binding domain
Fer2 PF00111 81 - 130 1.6e-06 2Fe-2S iron-sulfur cluster binding domain
NADH-G_4Fe-4S_3 PF10588 159 - 196 1.4e-16 NADH-ubiquinone oxidoreductase-G iron-sulfur binding region
Fer4_Nqo3 PF22117 212 - 278 4.8e-33 NADH-quinone oxidoreductase subunit 3, ferredoxin-like domain
Fer4_NDSU1 PF22151 291 - 341 5.9e-29 NADH-ubiquinone oxidoreductase NDSU1/NuoG-like, 4Fe-4S domain
Molybdopterin PF00384 347 - 667 1.4e-107 Molybdopterin oxidoreductase
NADH_dhqG_C PF09326 706 - 742 3e-13 NADH-ubiquinone oxidoreductase subunit G, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011004)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37510 AT5G37510
fragaria_vesca FvH4_1g30070
malus_domestica MD09G1268900.v1.1 MD17G1267300.v1.1
prunus_persica Prupe.3G165400_v2.0.a1
pyrus_communis pycom09g18080 pycom17g26640
rosa_chinensis RchiOBHm_Chr3g0491991
rosa_laevigata RLG00000022799 RLG00000022807
rosa_multiflora Rmu_sc0005238.1_g000005 Rmu_sc0036946.1_g000001
rosa_roxburghii Rroxscaffold_6G00392660
rosa_rugosa Rorug03G0251000
rosa_samantha Rh3AG301000 Rh3BG337200 Rh3CG334300 Rh3DG336600
rosa_wichuraiana Rw3G026610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 953
Acc36I ACCTGC 1 cut(s) 1945
Acc65I GGTACC 1 cut(s) 1343
AccB1I GGYRCC 2 cut(s) 1343, 2062
AccBSI CCGCTC 1 cut(s) 98
AccI GTMKAC 1 cut(s) 2082
AccII CGCG 1 cut(s) 588
AccIII TCCGGA 2 cut(s) 1280, 1386
AciI CCGC 9 cut(s) 98, 146, 165, 195, 586, 588, 744, 1683, 2011
AclWI GGATC 9 cut(s) 19, 567, 586, 606, 906, 919, 1272, 2037, 2057
AcoI YGGCCR 1 cut(s) 589
AcsI RAATTY 1 cut(s) 2173
AcuI CTGAAG 1 cut(s) 1932
AfaI GTAC 6 cut(s) 676, 1300, 1345, 1424, 1668, 1935
AgsI TTSAA 5 cut(s) 671, 1030, 1447, 1613, 1663
AjnI CCWGG 4 cut(s) 430, 1686, 1961, 2198
AjuI GAANNNNNNNTTGG 2 cut(s) 497, 529
AluBI AGCT 9 cut(s) 60, 118, 790, 1092, 1171, 1364, 1403, 1439, 2099
AluI AGCT 9 cut(s) 60, 118, 790, 1092, 1171, 1364, 1403, 1439, 2099
Alw21I GWGCWC 2 cut(s) 120, 1922
Alw26I GTCTC 3 cut(s) 106, 878, 1827
AlwI GGATC 9 cut(s) 19, 567, 586, 606, 906, 919, 1272, 2037, 2057
AlwNI CAGNNNCTG 2 cut(s) 1724, 1937
Ama87I CYCGRG 1 cut(s) 474
Aor13HI TCCGGA 2 cut(s) 1280, 1386
AoxI GGCC 9 cut(s) 27, 35, 147, 198, 291, 589, 1429, 1474, 1643
ApaI GGGCCC 1 cut(s) 202
ApeKI GCWGC 7 cut(s) 741, 1364, 1691, 1694, 1871, 1874, 2014
ApoI RAATTY 1 cut(s) 2173
ArsI GACNNNNNNTTYG 2 cut(s) 228, 260
Asp700I GAANNNNTTC 2 cut(s) 1205, 1658
Asp718I GGTACC 1 cut(s) 1343
AspLEI GCGC 4 cut(s) 416, 954, 1077, 1769
AsuC2I CCSGG 1 cut(s) 2047
AsuHPI GGTGA 5 cut(s) 496, 655, 1502, 1967, 2188
AsuII TTCGAA 3 cut(s) 922, 1256, 2207
AvaI CYCGRG 1 cut(s) 474
AvaII GGWCC 6 cut(s) 157, 183, 635, 718, 938, 1525
BaeGI GKGCMC 1 cut(s) 202
BamHI GGATCC 1 cut(s) 911
BanI GGYRCC 2 cut(s) 1343, 2062
BanII GRGCYC 4 cut(s) 120, 202, 829, 1990
BbsI GAAGAC 3 cut(s) 232, 653, 1397
Bbv12I GWGCWC 2 cut(s) 120, 1922
BbvCI CCTCAGC 1 cut(s) 56
BbvI GCAGC 7 cut(s) 753, 1351, 1678, 1706, 1883, 1886, 2001
BccI CCATC 9 cut(s) 239, 242, 481, 1016, 1064, 1117, 1186, 1559, 1756
BceAI ACGGC 3 cut(s) 348, 576, 890
BcgI CGANNNNNNTGC 2 cut(s) 1236, 1270
BciT130I CCWGG 4 cut(s) 432, 1688, 1963, 2200
BciVI GTATCC 2 cut(s) 245, 2137
BcnI CCSGG 1 cut(s) 2047
BcoDI GTCTC 3 cut(s) 106, 878, 1827
BfaI CTAG 4 cut(s) 72, 321, 1380, 1490
BfmI CTRYAG 6 cut(s) 554, 1110, 1692, 1869, 1938, 2163
BfoI RGCGCY 2 cut(s) 1078, 1770
BfuAI ACCTGC 1 cut(s) 1945
BfuI GTATCC 2 cut(s) 245, 2137
BglI GCCNNNNNGGC 2 cut(s) 1124, 1688
BglII AGATCT 1 cut(s) 169
Bme1390I CCNGG 5 cut(s) 432, 1688, 1963, 2047, 2200
Bme18I GGWCC 6 cut(s) 157, 183, 635, 718, 938, 1525
BmeT110I CYCGRG 1 cut(s) 474
BmrFI CCNGG 5 cut(s) 432, 1688, 1963, 2047, 2200
BoxI GACNNNNGTC 1 cut(s) 764
BpiI GAAGAC 3 cut(s) 232, 653, 1397
BpmI CTGGAG 2 cut(s) 104, 1419
Bpu10I CCTNAGC 1 cut(s) 56
Bpu14I TTCGAA 3 cut(s) 922, 1256, 2207
BpuEI CTTGAG 1 cut(s) 1334
BpuMI CCSGG 1 cut(s) 2047
Bsa29I ATCGAT 1 cut(s) 2114
BsaAI YACGTR 1 cut(s) 1903
BsaBI GATNNNNATC 3 cut(s) 627, 809, 1269
BsaI GGTCTC 3 cut(s) 106, 878, 1827
BsaWI WCCGGW 2 cut(s) 1280, 1386
BsaXI ACNNNNNCTCC 2 cut(s) 439, 469
Bse118I RCCGGY 3 cut(s) 305, 339, 353
Bse1I ACTGG 1 cut(s) 1976
Bse3DI GCAATG 1 cut(s) 1302
Bse8I GATNNNNATC 3 cut(s) 627, 809, 1269
BseAI TCCGGA 2 cut(s) 1280, 1386
BseBI CCWGG 4 cut(s) 432, 1688, 1963, 2200
BseCI ATCGAT 1 cut(s) 2114
BseGI GGATG 4 cut(s) 508, 1597, 1860, 1972
BseJI GATNNNNATC 3 cut(s) 627, 809, 1269
BseMI GCAATG 1 cut(s) 1302
BseMII CTCAG 6 cut(s) 70, 591, 1173, 1493, 1986, 2147
BseNI ACTGG 1 cut(s) 1976
BseRI GAGGAG 4 cut(s) 44, 168, 555, 1160
BseSI GKGCMC 1 cut(s) 202
BseX3I CGGCCG 1 cut(s) 589
BseXI GCAGC 7 cut(s) 753, 1351, 1678, 1706, 1883, 1886, 2001
BseYI CCCAGC 4 cut(s) 150, 707, 1347, 1565
Bsh1236I CGCG 1 cut(s) 588
Bsh1285I CGRYCG 2 cut(s) 351, 592
BshFI GGCC 9 cut(s) 29, 37, 149, 200, 293, 591, 1431, 1476, 1645
BshNI GGYRCC 2 cut(s) 1343, 2062
BshVI ATCGAT 1 cut(s) 2114
BsiEI CGRYCG 2 cut(s) 351, 592
BsiHKAI GWGCWC 2 cut(s) 120, 1922
BsiHKCI CYCGRG 1 cut(s) 474
BsiSI CCGG 8 cut(s) 113, 155, 306, 340, 354, 1281, 1387, 2047
BslFI GGGAC 3 cut(s) 772, 944, 2173
BsmAI GTCTC 3 cut(s) 106, 878, 1827
BsmFI GGGAC 3 cut(s) 772, 944, 2173
BsmI GAATGC 1 cut(s) 1877
BsnI GGCC 9 cut(s) 29, 37, 149, 200, 293, 591, 1431, 1476, 1645
Bso31I GGTCTC 3 cut(s) 106, 878, 1827
BsoBI CYCGRG 1 cut(s) 474
Bsp119I TTCGAA 3 cut(s) 922, 1256, 2207
Bsp120I GGGCCC 1 cut(s) 198
Bsp1286I GDGCHC 5 cut(s) 120, 202, 829, 1922, 1990
Bsp13I TCCGGA 2 cut(s) 1280, 1386
Bsp1407I TGTACA 1 cut(s) 674
Bsp19I CCATGG 1 cut(s) 1617
BspACI CCGC 9 cut(s) 98, 146, 165, 195, 586, 588, 744, 1683, 2011
BspANI GGCC 9 cut(s) 29, 37, 149, 200, 293, 591, 1431, 1476, 1645
BspCNI CTCAG 6 cut(s) 69, 592, 1174, 1492, 1987, 2146
BspDI ATCGAT 1 cut(s) 2114
BspEI TCCGGA 2 cut(s) 1280, 1386
BspFNI CGCG 1 cut(s) 588
BspHI TCATGA 1 cut(s) 1456
BspMAI CTGCAG 5 cut(s) 558, 1696, 1873, 1942, 2167
BspMI ACCTGC 1 cut(s) 1945
BspPI GGATC 9 cut(s) 19, 567, 586, 606, 906, 919, 1272, 2037, 2057
BspT104I TTCGAA 3 cut(s) 922, 1256, 2207
BspT107I GGYRCC 2 cut(s) 1343, 2062
BspTNI GGTCTC 3 cut(s) 106, 878, 1827
BsrBI CCGCTC 1 cut(s) 98
BsrDI GCAATG 1 cut(s) 1302
BsrFI RCCGGY 3 cut(s) 305, 339, 353
BsrGI TGTACA 1 cut(s) 674
BsrI ACTGG 1 cut(s) 1976
BssAI RCCGGY 3 cut(s) 305, 339, 353
BssT1I CCWWGG 7 cut(s) 270, 513, 721, 1352, 1413, 1617, 1705
Bst2UI CCWGG 4 cut(s) 432, 1688, 1963, 2200
Bst4CI ACNGT 8 cut(s) 283, 652, 843, 1262, 1396, 1933, 1951, 2059
Bst6I CTCTTC 1 cut(s) 1025
BstAUI TGTACA 1 cut(s) 674
BstBAI YACGTR 1 cut(s) 1903
BstBI TTCGAA 3 cut(s) 922, 1256, 2207
BstC8I GCNNGC 9 cut(s) 18, 291, 307, 423, 1094, 1401, 2005, 2009, 2097
BstDEI CTNAG 6 cut(s) 56, 600, 1182, 1479, 1995, 2133
BstDSI CCRYGG 2 cut(s) 586, 1617
BstENI CCTNNNNNAGG 1 cut(s) 377
BstF5I GGATG 4 cut(s) 508, 1597, 1860, 1972
BstFNI CGCG 1 cut(s) 588
BstH2I RGCGCY 2 cut(s) 1078, 1770
BstHHI GCGC 4 cut(s) 416, 954, 1077, 1769
BstMAI GTCTC 3 cut(s) 106, 878, 1827
BstMCI CGRYCG 2 cut(s) 351, 592
BstNI CCWGG 4 cut(s) 432, 1688, 1963, 2200
BstNSI RCATGY 1 cut(s) 730
BstPAI GACNNNNGTC 1 cut(s) 764
BstSCI CCNGG 5 cut(s) 430, 1686, 1961, 2045, 2198
BstSFI CTRYAG 6 cut(s) 554, 1110, 1692, 1869, 1938, 2163
BstSLI GKGCMC 1 cut(s) 202
BstSNI TACGTA 1 cut(s) 1903
BstUI CGCG 1 cut(s) 588
BstV1I GCAGC 7 cut(s) 753, 1351, 1678, 1706, 1883, 1886, 2001
BstV2I GAAGAC 3 cut(s) 232, 653, 1397
BstX2I RGATCY 4 cut(s) 169, 578, 611, 911
BstYI RGATCY 4 cut(s) 169, 578, 611, 911
BstZI CGGCCG 1 cut(s) 589
Bsu15I ATCGAT 1 cut(s) 2114
BsuI GTATCC 2 cut(s) 245, 2137
BsuRI GGCC 9 cut(s) 29, 37, 149, 200, 293, 591, 1431, 1476, 1645
BsuTUI ATCGAT 1 cut(s) 2114
BtgI CCRYGG 2 cut(s) 586, 1617
BtsCI GGATG 4 cut(s) 508, 1597, 1860, 1972
BtsIMutI CAGTG 5 cut(s) 597, 1196, 1888, 1908, 2064
BveI ACCTGC 1 cut(s) 1945
Cac8I GCNNGC 9 cut(s) 18, 291, 307, 423, 1094, 1401, 2005, 2009, 2097
CaiI CAGNNNCTG 2 cut(s) 1724, 1937
CciI TCATGA 1 cut(s) 1456
CfoI GCGC 4 cut(s) 416, 954, 1077, 1769
Cfr10I RCCGGY 3 cut(s) 305, 339, 353
Cfr42I CCGCGG 1 cut(s) 589
ClaI ATCGAT 1 cut(s) 2114
CseI GACGC 1 cut(s) 129
Csp6I GTAC 6 cut(s) 675, 1299, 1344, 1423, 1667, 1934
CspCI CAANNNNNGTGG 2 cut(s) 2137, 2172
CviAII CATG 9 cut(s) 31, 418, 427, 436, 727, 1457, 1618, 1829, 2182
CviQI GTAC 6 cut(s) 675, 1299, 1344, 1423, 1667, 1934
DdeI CTNAG 6 cut(s) 56, 600, 1182, 1479, 1995, 2133
DraI TTTAAA 1 cut(s) 853
EaeI YGGCCR 1 cut(s) 589
EagI CGGCCG 1 cut(s) 589
Eam1104I CTCTTC 1 cut(s) 1025
EarI CTCTTC 1 cut(s) 1025
Ecl136II GAGCTC 1 cut(s) 118
EclXI CGGCCG 1 cut(s) 589
Eco105I TACGTA 1 cut(s) 1903
Eco130I CCWWGG 7 cut(s) 270, 513, 721, 1352, 1413, 1617, 1705
Eco147I AGGCCT 2 cut(s) 293, 1645
Eco24I GRGCYC 4 cut(s) 120, 202, 829, 1990
Eco31I GGTCTC 3 cut(s) 106, 878, 1827
Eco32I GATATC 3 cut(s) 316, 811, 996
Eco47I GGWCC 6 cut(s) 157, 183, 635, 718, 938, 1525
Eco52I CGGCCG 1 cut(s) 589
Eco53kI GAGCTC 1 cut(s) 118
Eco57I CTGAAG 1 cut(s) 1932
Eco88I CYCGRG 1 cut(s) 474
EcoICRI GAGCTC 1 cut(s) 118
EcoNI CCTNNNNNAGG 1 cut(s) 377
EcoO109I RGGNCCY 3 cut(s) 198, 718, 1475
EcoRII CCWGG 4 cut(s) 430, 1686, 1961, 2198
EcoRV GATATC 3 cut(s) 316, 811, 996
EcoT14I CCWWGG 7 cut(s) 270, 513, 721, 1352, 1413, 1617, 1705
EcoT22I ATGCAT 1 cut(s) 1807
EcoT38I GRGCYC 4 cut(s) 120, 202, 829, 1990
ErhI CCWWGG 7 cut(s) 270, 513, 721, 1352, 1413, 1617, 1705
FaeI CATG 9 cut(s) 34, 421, 430, 439, 730, 1460, 1621, 1832, 2185
FaqI GGGAC 3 cut(s) 772, 944, 2173
FatI CATG 9 cut(s) 30, 417, 426, 435, 726, 1456, 1617, 1828, 2181
FauI CCCGC 2 cut(s) 172, 188
FauNDI CATATG 1 cut(s) 2020
FblI GTMKAC 1 cut(s) 2082
FokI GGATG 4 cut(s) 495, 1604, 1847, 1979
FriOI GRGCYC 4 cut(s) 120, 202, 829, 1990
FspBI CTAG 4 cut(s) 72, 321, 1380, 1490
FspI TGCGCA 1 cut(s) 953
GlaI GCGC 4 cut(s) 415, 953, 1076, 1768
GsaI CCCAGC 4 cut(s) 154, 711, 1351, 1569
GsuI CTGGAG 2 cut(s) 104, 1419
HaeII RGCGCY 2 cut(s) 1078, 1770
HaeIII GGCC 9 cut(s) 29, 37, 149, 200, 293, 591, 1431, 1476, 1645
HapII CCGG 8 cut(s) 113, 155, 306, 340, 354, 1281, 1387, 2047
HgaI GACGC 1 cut(s) 129
HhaI GCGC 4 cut(s) 416, 954, 1077, 1769
Hin1II CATG 9 cut(s) 34, 421, 430, 439, 730, 1460, 1621, 1832, 2185
Hin6I GCGC 4 cut(s) 414, 952, 1075, 1767
HinP1I GCGC 4 cut(s) 414, 952, 1075, 1767
HpaII CCGG 8 cut(s) 113, 155, 306, 340, 354, 1281, 1387, 2047
HphI GGTGA 5 cut(s) 496, 655, 1502, 1967, 2188
Hpy166II GTNNAC 4 cut(s) 208, 1756, 1906, 2083
Hpy8I GTNNAC 4 cut(s) 208, 1756, 1906, 2083
Hpy99I CGWCG 4 cut(s) 145, 248, 407, 1509
HpyAV CCTTC 8 cut(s) 88, 97, 463, 473, 1783, 1874, 1886, 1907
HpyCH4III ACNGT 8 cut(s) 283, 652, 843, 1262, 1396, 1933, 1951, 2059
HpyCH4IV ACGT 4 cut(s) 1425, 1504, 1857, 1902
HpyF3I CTNAG 6 cut(s) 56, 600, 1182, 1479, 1995, 2133
HpySE526I ACGT 4 cut(s) 1425, 1504, 1857, 1902
Hsp92II CATG 9 cut(s) 34, 421, 430, 439, 730, 1460, 1621, 1832, 2185
HspAI GCGC 4 cut(s) 414, 952, 1075, 1767
Kpn2I TCCGGA 2 cut(s) 1280, 1386
KpnI GGTACC 1 cut(s) 1347
KroI GCCGGC 1 cut(s) 305
KroNI GCCGGC 1 cut(s) 307
KspI CCGCGG 1 cut(s) 589
LmnI GCTCC 6 cut(s) 57, 65, 115, 123, 824, 2241
Lsp1109I GCAGC 7 cut(s) 753, 1351, 1678, 1706, 1883, 1886, 2001
MaeI CTAG 4 cut(s) 72, 321, 1380, 1490
MaeII ACGT 4 cut(s) 1425, 1504, 1857, 1902
MaeIII GTNAC 1 cut(s) 1508
MbiI CCGCTC 1 cut(s) 98
MflI RGATCY 4 cut(s) 169, 578, 611, 911
MhlI GDGCHC 5 cut(s) 120, 202, 829, 1922, 1990
MlyI GAGTC 4 cut(s) 652, 1000, 1193, 1748
MmeI TCCRAC 3 cut(s) 889, 939, 1970
Mph1103I ATGCAT 1 cut(s) 1807
MreI CGCCGGCG 1 cut(s) 305
MroI TCCGGA 2 cut(s) 1280, 1386
MroNI GCCGGC 1 cut(s) 305
MroXI GAANNNNTTC 2 cut(s) 1205, 1658
MspA1I CMGCKG 3 cut(s) 588, 744, 1439
MspI CCGG 8 cut(s) 113, 155, 306, 340, 354, 1281, 1387, 2047
MspR9I CCNGG 5 cut(s) 432, 1688, 1963, 2047, 2200
Mva1269I GAATGC 1 cut(s) 1877
MvaI CCWGG 4 cut(s) 432, 1688, 1963, 2200
MvnI CGCG 1 cut(s) 588
NaeI GCCGGC 1 cut(s) 307
NciI CCSGG 1 cut(s) 2047
NcoI CCATGG 1 cut(s) 1617
NdeI CATATG 1 cut(s) 2020
NgoMIV GCCGGC 1 cut(s) 305
NlaIII CATG 9 cut(s) 34, 421, 430, 439, 730, 1460, 1621, 1832, 2185
NmeAIII GCCGAG 3 cut(s) 959, 1144, 1446
NmuCI GTSAC 1 cut(s) 1508
NsbI TGCGCA 1 cut(s) 953
NsiI ATGCAT 1 cut(s) 1807
NspI RCATGY 1 cut(s) 730
NspV TTCGAA 3 cut(s) 922, 1256, 2207
PaeR7I CTCGAG 1 cut(s) 474
PagI TCATGA 1 cut(s) 1456
PceI AGGCCT 2 cut(s) 293, 1645
PcsI WCGNNNNNNNCGW 2 cut(s) 20, 240
PctI GAATGC 1 cut(s) 1877
PdiI GCCGGC 1 cut(s) 307
PdmI GAANNNNTTC 2 cut(s) 1205, 1658
PfoI TCCNGGA 1 cut(s) 2045
Ple19I CGATCG 1 cut(s) 351
PleI GAGTC 4 cut(s) 652, 1000, 1192, 1747
PpsI GAGTC 4 cut(s) 652, 1000, 1192, 1747
Ppu21I YACGTR 1 cut(s) 1903
PpuMI RGGWCCY 1 cut(s) 718
PshAI GACNNNNGTC 1 cut(s) 764
Psp124BI GAGCTC 1 cut(s) 120
Psp5II RGGWCCY 1 cut(s) 718
Psp6I CCWGG 4 cut(s) 430, 1686, 1961, 2198
PspFI CCCAGC 4 cut(s) 150, 707, 1347, 1565
PspGI CCWGG 4 cut(s) 430, 1686, 1961, 2198
PspOMI GGGCCC 1 cut(s) 198
PspPPI RGGWCCY 1 cut(s) 718
PsrI GAACNNNNNNTAC 2 cut(s) 1926, 1958
PstI CTGCAG 5 cut(s) 558, 1696, 1873, 1942, 2167
PstNI CAGNNNCTG 2 cut(s) 1724, 1937
PsuI RGATCY 4 cut(s) 169, 578, 611, 911
PvuI CGATCG 1 cut(s) 351
PvuII CAGCTG 1 cut(s) 1439
RsaI GTAC 6 cut(s) 676, 1300, 1345, 1424, 1668, 1935
RsaNI GTAC 6 cut(s) 675, 1299, 1344, 1423, 1667, 1934
SacI GAGCTC 1 cut(s) 120
SacII CCGCGG 1 cut(s) 589
SchI GAGTC 4 cut(s) 652, 1000, 1193, 1748
ScrFI CCNGG 5 cut(s) 432, 1688, 1963, 2047, 2200
SduI GDGCHC 5 cut(s) 120, 202, 829, 1922, 1990
SfcI CTRYAG 6 cut(s) 554, 1110, 1692, 1869, 1938, 2163
Sfr274I CTCGAG 1 cut(s) 474
Sfr303I CCGCGG 1 cut(s) 589
SfuI TTCGAA 3 cut(s) 922, 1256, 2207
SgrAI CRCCGGYG 1 cut(s) 305
SgrBI CCGCGG 1 cut(s) 589
SinI GGWCC 6 cut(s) 157, 183, 635, 718, 938, 1525
SlaI CTCGAG 1 cut(s) 474
SmlI CTYRAG 2 cut(s) 474, 1313
SmoI CTYRAG 2 cut(s) 474, 1313
SnaBI TACGTA 1 cut(s) 1903
SseBI AGGCCT 2 cut(s) 293, 1645
SsiI CCGC 9 cut(s) 98, 146, 165, 195, 586, 588, 744, 1683, 2011
SspMI CTAG 4 cut(s) 72, 321, 1380, 1490
SstI GAGCTC 1 cut(s) 120
StuI AGGCCT 2 cut(s) 293, 1645
StyD4I CCNGG 5 cut(s) 430, 1686, 1961, 2045, 2198
StyI CCWWGG 7 cut(s) 270, 513, 721, 1352, 1413, 1617, 1705
TaaI ACNGT 8 cut(s) 283, 652, 843, 1262, 1396, 1933, 1951, 2059
TaiI ACGT 4 cut(s) 1428, 1507, 1860, 1905
TaqII GACCGA 3 cut(s) 1513, 1851, 2201
TatI WGTACW 2 cut(s) 674, 1666
TauI GCSGC 4 cut(s) 149, 591, 1685, 2014
TscAI CASTG 5 cut(s) 604, 1203, 1888, 1915, 2064
TseFI GTSAC 1 cut(s) 1508
TseI GCWGC 7 cut(s) 741, 1364, 1691, 1694, 1871, 1874, 2014
Tsp45I GTSAC 1 cut(s) 1508
TspDTI ATGAA 5 cut(s) 452, 518, 999, 1307, 2097
TspGWI ACGGA 1 cut(s) 604
TspRI CASTG 5 cut(s) 604, 1203, 1888, 1915, 2064
VpaK11BI GGWCC 6 cut(s) 157, 183, 635, 718, 938, 1525
XagI CCTNNNNNAGG 1 cut(s) 377
XapI RAATTY 1 cut(s) 2173
XbaI TCTAGA 1 cut(s) 1489
XceI RCATGY 1 cut(s) 730
XcmI CCANNNNNNNNNTGG 1 cut(s) 2137
XhoI CTCGAG 1 cut(s) 474
XmiI GTMKAC 1 cut(s) 2082
XmnI GAANNNNTTC 2 cut(s) 1205, 1658
XspI CTAG 4 cut(s) 72, 321, 1380, 1490
Zsp2I ATGCAT 1 cut(s) 1807
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.