Rh3CG334300

Belongs to the complex I 75 kDa subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0011004
Physical Location & Seq
Reverse (-)
37259841 .. 37265991
6151 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG334300.1

Sequence Viewer

Length: 2259 bp
ATGGGGTTGGGATCGCTTGCTTCCAGGGCCCTAAGGCCCAGGGCCTCAAGGCTCCTCCTCGGCTCCCAAAACCCTGGACCCTTCTTCTCCTTCCAGAGAACCATGGTCACATCCACCCCGGAGCTCAAGAATCCGGAGCCGTCCACGGCCGCCCAGCCCGAGCCCCCCGCCGCCGCCGACCTCCCGCCCCGGACCCCGGTCGGTGGGGCCCGAATCCACTTCGCGAACCCGGACGACGCGATCGAGGTGTTCGTGGACGGATACCCTGTGAAAATCCCTAAGGGCATGTCCGTGTTGCAAGCGTGTGAGGTCGCCGGTGTTGACATCCCTAGGTTTTGCTACCACAGCAGATTGTCCATTGCCGGTAATTGCCGTATGTGTCTCGTCGAGGTTGAGAAGTCACCCAAGCCCGTCGCCTCTTGCGCCATGCCTGCTATGCCTGGTATGAAAATTAAGACTGACACTCCAATAGCCAAGAAGGCTCGCGAAGGGGTGATGGAGTTTCTGTTGATGAATCATCCTTTGGATTGTCCAATTTGTGATCAGGGTGGAGAATGTGATCTCCAGGATCAGTCTATGGCATTTGGATCTGATCGTGGCCGATTCACTGAAATGAAGAGATCAGTTGTGGACAAGAATCTTGGTCCTTTGGTCAAGACTGTCATGACTCGCTGTATTCAATGTACAAGGTGCGTTAGGTTTGCAACAGAGGTCGCTGGGGTTCAGGACCTTGGAATGTTAGGCCGTGGAAGTGGAGAGGAAATTGGGACTTACGTTGAAAAGCTTATGACAAGTGAACTTTCAGGAAATGTTATAGATATCTGTCCTGTAGGAGCCCTAACCTCAAAACCATTTGCATTTAAAGCTCGTAATTGGGAATTAAAGGGAACAGAGACCATTGATGTTACTGATGCTGTTGGATCCAACATTCGAATTGATAGCAGAGGTCCCGAAGTCATGCGCATTGTTCCACGCTTAAATGAGGACATAAATGAGGAATGGATATCAGACAAGACTCGATTTTGTTATGACGGTTTGAAGAGGCAAAGGTTGAATGACCCTATGATTCGTGGTGCTGATGGACGCTTTAAGCCCGTGAGCTGGCGTGATGCCCTTGCTGTTGTTGCTGAGATGGCTCATAAAGTTAAGCCAGAAGAAATTGTTGGGGTTGCTGGTCAGCTGTCTGATGCCGAGTCCATGATGGCACTAAAAGACTTTTTAAATAAAATGGGATCTAACAATGTGTGGTGCGAAGGAAATGGTTCCAATCGTGATGCTGATCTTCGATCTGGATATATAATGAACAGTAGCATTGCTGGACTTGAGAAGGCAGATGCTTTTCTATTGGTTGGCACCCAGCCAAGAGTAGAAGCTGCTATTGTAAATGCTAGAATCCGGAAGACAGTGTTATCTAACCAAGCCAAGGTTGGGTATGTTGGTCCTGAAGCTGATTTAAACTATGAACACCAGCATCTTGGAACAGGCCCCGATACACTTCTAGAACTTGCTGAGCGTCGCCACCCCTTTTCCTCAGTCCTCTTAAATGCCAAAAACCCTGCCATTATTGTTGGTGCTGGGCTGTTTGAGAGAAAAGACAAGGATGCAATTTTCTCAGCCCTTGAAACCATTGCCAAATATGCTGATGCTGTAAGGCCTGATTGGAATGGATTTAATGTACTGCTTCTCAAAGCCGCCCAGGCTGCAGCACTTGACCTTGGACTTGTGCCAGAATCTGAAAACAGCATTGAGTCTGCAAAATTTGTTTACCTGATGGGTGCTGATGATGTTAACTTAGACAAGGTTCCATCTGATGCATTTGTGGTTTATCAAGGGCATCATGGCGACCGGGGTGTGTATCGGGCTAATGTCATCCTCCCTGCAGCAGCGTTCAGTGAAAAGGAAGGAACATATGTAAATACTGAAGGGTGCTCTCAGCAAACACTGCCTGCAGTTCCAACTGTCGGTGATGCCAGGGACGACTGGAAGATACTCCGTGCTTTATCTGAGGTAGCAGGCGTGAAGCTGCCTTACGATACAGTTGGGACCCTCCAATCCCGGATAAAGACCGTGGCACCAAACATCTTAAACATAGACGAGAGAGAGCCAGCTACTTTTTCATTTTCGATTAAGCCTGAGACCACTCAGAAAATGGATTTGACTCCATTTGGTACTGCTGTGGATAATTTCTATATGACCGATTCCATCACCAGGGCATCAAAGATAATGGCACAATGCAGTGCGTTGCTATTGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko04723 ko04932 ko05010 ko05012 ko05016 map00190 map01100 map04714 map04723 map04932 map05010 map05012 map05016
Pfam Domains
Protein Families
Fer2_4 Molybdopterin NADH-G_4Fe-4S_3 NADH_dhqG_C

Protein Analysis

752

Amino Acids

81.82

Weight (kDa)

6.01

Isoelectric Point (pI)

35.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer2_4 PF13510 79 - 155 6.4e-19 2Fe-2S iron-sulfur cluster binding domain
Fer2 PF00111 84 - 133 7.8e-07 2Fe-2S iron-sulfur cluster binding domain
NADH-G_4Fe-4S_3 PF10588 162 - 199 1.4e-16 NADH-ubiquinone oxidoreductase-G iron-sulfur binding region
Fer4_Nqo3 PF22117 215 - 281 4.9e-33 NADH-quinone oxidoreductase subunit 3, ferredoxin-like domain
Fer4_NDSU1 PF22151 294 - 344 5.7e-30 NADH-ubiquinone oxidoreductase NDSU1/NuoG-like, 4Fe-4S domain
Molybdopterin PF00384 350 - 670 4.3e-109 Molybdopterin oxidoreductase
NADH_dhqG_C PF09326 710 - 745 1.1e-11 NADH-ubiquinone oxidoreductase subunit G, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011004)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37510 AT5G37510
fragaria_vesca FvH4_1g30070
malus_domestica MD09G1268900.v1.1 MD17G1267300.v1.1
prunus_persica Prupe.3G165400_v2.0.a1
pyrus_communis pycom09g18080 pycom17g26640
rosa_chinensis RchiOBHm_Chr3g0491991
rosa_laevigata RLG00000022799 RLG00000022807
rosa_multiflora Rmu_sc0005238.1_g000005 Rmu_sc0036946.1_g000001
rosa_roxburghii Rroxscaffold_6G00392660
rosa_rugosa Rorug03G0251000
rosa_samantha Rh3AG301000 Rh3BG337200 Rh3CG334300 Rh3DG336600
rosa_wichuraiana Rw3G026610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 962
AccB1I GGYRCC 2 cut(s) 1352, 2071
AccII CGCG 3 cut(s) 224, 239, 486
AccIII TCCGGA 2 cut(s) 133, 1395
AciI CCGC 6 cut(s) 150, 168, 171, 174, 185, 1692
AclWI GGATC 6 cut(s) 19, 576, 595, 915, 928, 1240
AcoI YGGCCR 2 cut(s) 147, 598
AcsI RAATTY 1 cut(s) 1757
AcuI CTGAAG 2 cut(s) 1464, 1941
AfaI GTAC 3 cut(s) 685, 1677, 2170
AfiI CCNNNNNNNGG 7 cut(s) 196, 203, 1101, 1423, 1428, 1961, 2208
AgsI TTSAA 6 cut(s) 680, 779, 1039, 1054, 1622, 2251
AjnI CCWGG 8 cut(s) 23, 38, 73, 439, 564, 1695, 1970, 2207
AjuI GAANNNNNNNTTGG 4 cut(s) 506, 538, 1146, 1178
AluBI AGCT 9 cut(s) 124, 784, 866, 1101, 1180, 1373, 1448, 2023, 2108
AluI AGCT 9 cut(s) 124, 784, 866, 1101, 1180, 1373, 1448, 2023, 2108
Alw21I GWGCWC 2 cut(s) 126, 1931
Alw26I GTCTC 3 cut(s) 386, 887, 2129
AlwI GGATC 6 cut(s) 19, 576, 595, 915, 928, 1240
AlwNI CAGNNNCTG 1 cut(s) 1733
Ama87I CYCGRG 1 cut(s) 158
Aor13HI TCCGGA 2 cut(s) 133, 1395
AoxI GGCC 9 cut(s) 27, 35, 42, 147, 207, 598, 742, 1483, 1652
ApaI GGGCCC 2 cut(s) 31, 211
ApeKI GCWGC 6 cut(s) 1373, 1700, 1703, 1880, 1883, 2023
ApoI RAATTY 1 cut(s) 1757
Asp700I GAANNNNTTC 1 cut(s) 1261
AspA2I CCTAGG 1 cut(s) 329
AspLEI GCGC 2 cut(s) 425, 963
AsuC2I CCSGG 6 cut(s) 119, 190, 197, 230, 1847, 2056
AsuHPI GGTGA 4 cut(s) 393, 505, 1976, 2197
AsuII TTCGAA 1 cut(s) 931
AvaI CYCGRG 1 cut(s) 158
AvaII GGWCC 7 cut(s) 77, 192, 644, 727, 947, 1439, 2043
AvrII CCTAGG 1 cut(s) 329
AxyI CCTNAGG 2 cut(s) 32, 279
BaeGI GKGCMC 2 cut(s) 31, 211
BaeI ACNNNNGTAYC 2 cut(s) 2160, 2193
BamHI GGATCC 1 cut(s) 920
BanI GGYRCC 2 cut(s) 1352, 2071
BanII GRGCYC 5 cut(s) 31, 126, 165, 211, 838
BarI GAAGNNNNNNTAC 2 cut(s) 2012, 2044
BbsI GAAGAC 1 cut(s) 1406
Bbv12I GWGCWC 2 cut(s) 126, 1931
BbvI GCAGC 6 cut(s) 1360, 1687, 1715, 1892, 1895, 2010
BccI CCATC 7 cut(s) 490, 1073, 1126, 1195, 1765, 1813, 2210
BceAI ACGGC 4 cut(s) 124, 162, 357, 729
BciT130I CCWGG 8 cut(s) 25, 40, 75, 441, 566, 1697, 1972, 2209
BciVI GTATCC 1 cut(s) 254
BclI TGATCA 1 cut(s) 541
BcnI CCSGG 6 cut(s) 119, 190, 197, 230, 1847, 2056
BcoDI GTCTC 3 cut(s) 386, 887, 2129
BfaI CTAG 3 cut(s) 330, 1389, 1499
BfmI CTRYAG 4 cut(s) 828, 1701, 1878, 1947
BfuI GTATCC 1 cut(s) 254
BglI GCCNNNNNGGC 2 cut(s) 479, 1697
BlnI CCTAGG 1 cut(s) 329
BlpI GCTNAGC 1 cut(s) 1509
Bme18I GGWCC 7 cut(s) 77, 192, 644, 727, 947, 1439, 2043
BmeT110I CYCGRG 1 cut(s) 158
BoxI GACNNNNGTC 1 cut(s) 197
BpiI GAAGAC 1 cut(s) 1406
BpmI CTGGAG 1 cut(s) 548
Bpu1102I GCTNAGC 1 cut(s) 1509
Bpu14I TTCGAA 1 cut(s) 931
BpuEI CTTGAG 3 cut(s) 31, 110, 1343
BpuMI CCSGG 6 cut(s) 119, 190, 197, 230, 1847, 2056
BsaBI GATNNNNATC 1 cut(s) 1278
BsaI GGTCTC 2 cut(s) 887, 2129
BsaWI WCCGGW 2 cut(s) 133, 1395
BsaXI ACNNNNNCTCC 4 cut(s) 448, 478, 491, 521
Bsc4I CCNNNNNNNGG 7 cut(s) 196, 203, 1101, 1423, 1428, 1961, 2208
Bse118I RCCGGY 2 cut(s) 314, 362
Bse1I ACTGG 1 cut(s) 1985
Bse21I CCTNAGG 2 cut(s) 32, 279
Bse3DI GCAATG 3 cut(s) 357, 1311, 1626
Bse8I GATNNNNATC 1 cut(s) 1278
BseAI TCCGGA 2 cut(s) 133, 1395
BseBI CCWGG 8 cut(s) 25, 40, 75, 441, 566, 1697, 1972, 2209
BseGI GGATG 5 cut(s) 110, 324, 517, 1606, 1869
BseJI GATNNNNATC 1 cut(s) 1278
BseLI CCNNNNNNNGG 7 cut(s) 196, 203, 1101, 1423, 1428, 1961, 2208
BseMI GCAATG 3 cut(s) 357, 1311, 1626
BseMII CTCAG 8 cut(s) 1119, 1500, 1545, 1626, 1946, 1995, 2124, 2156
BseNI ACTGG 1 cut(s) 1985
BseRI GAGGAG 2 cut(s) 44, 47
BseSI GKGCMC 2 cut(s) 31, 211
BseX3I CGGCCG 1 cut(s) 147
BseXI GCAGC 6 cut(s) 1360, 1687, 1715, 1892, 1895, 2010
BseYI CCCAGC 4 cut(s) 153, 716, 1356, 1574
Bsh1236I CGCG 3 cut(s) 224, 239, 486
Bsh1285I CGRYCG 4 cut(s) 150, 201, 243, 1846
BshFI GGCC 9 cut(s) 29, 37, 44, 149, 209, 600, 744, 1485, 1654
BshNI GGYRCC 2 cut(s) 1352, 2071
BsiEI CGRYCG 4 cut(s) 150, 201, 243, 1846
BsiHKAI GWGCWC 2 cut(s) 126, 1931
BsiHKCI CYCGRG 1 cut(s) 158
BslFI GGGAC 4 cut(s) 781, 933, 1988, 2056
BslI CCNNNNNNNGG 7 cut(s) 196, 203, 1101, 1423, 1428, 1961, 2208
BsmAI GTCTC 3 cut(s) 386, 887, 2129
BsmFI GGGAC 4 cut(s) 781, 933, 1988, 2056
BsnI GGCC 9 cut(s) 29, 37, 44, 149, 209, 600, 744, 1485, 1654
Bso31I GGTCTC 2 cut(s) 887, 2129
BsoBI CYCGRG 1 cut(s) 158
Bsp119I TTCGAA 1 cut(s) 931
Bsp120I GGGCCC 2 cut(s) 27, 207
Bsp1286I GDGCHC 6 cut(s) 31, 126, 165, 211, 838, 1931
Bsp13I TCCGGA 2 cut(s) 133, 1395
Bsp1407I TGTACA 1 cut(s) 683
Bsp1720I GCTNAGC 1 cut(s) 1509
Bsp19I CCATGG 1 cut(s) 102
Bsp68I TCGCGA 2 cut(s) 224, 486
BspACI CCGC 6 cut(s) 150, 168, 171, 174, 185, 1692
BspANI GGCC 9 cut(s) 29, 37, 44, 149, 209, 600, 744, 1485, 1654
BspCNI CTCAG 8 cut(s) 1120, 1501, 1544, 1625, 1945, 1996, 2125, 2155
BspEI TCCGGA 2 cut(s) 133, 1395
BspFNI CGCG 3 cut(s) 224, 239, 486
BspHI TCATGA 1 cut(s) 663
BspMAI CTGCAG 3 cut(s) 1705, 1882, 1951
BspPI GGATC 6 cut(s) 19, 576, 595, 915, 928, 1240
BspT104I TTCGAA 1 cut(s) 931
BspT107I GGYRCC 2 cut(s) 1352, 2071
BspTNI GGTCTC 2 cut(s) 887, 2129
BsrDI GCAATG 3 cut(s) 357, 1311, 1626
BsrFI RCCGGY 2 cut(s) 314, 362
BsrGI TGTACA 1 cut(s) 683
BsrI ACTGG 1 cut(s) 1985
BssAI RCCGGY 2 cut(s) 314, 362
BssT1I CCWWGG 5 cut(s) 102, 329, 730, 1422, 1714
Bst2UI CCWGG 8 cut(s) 25, 40, 75, 441, 566, 1697, 1972, 2209
Bst4CI ACNGT 7 cut(s) 661, 1034, 1307, 1405, 1960, 2038, 2068
Bst6I CTCTTC 2 cut(s) 611, 1034
BstAPI GCANNNNNTGC 1 cut(s) 1942
BstAUI TGTACA 1 cut(s) 683
BstBI TTCGAA 1 cut(s) 931
BstC8I GCNNGC 8 cut(s) 18, 300, 432, 484, 1103, 1947, 2014, 2106
BstDSI CCRYGG 4 cut(s) 102, 144, 745, 2067
BstF5I GGATG 5 cut(s) 110, 324, 517, 1606, 1869
BstFNI CGCG 3 cut(s) 224, 239, 486
BstHHI GCGC 2 cut(s) 425, 963
BstMAI GTCTC 3 cut(s) 386, 887, 2129
BstMCI CGRYCG 4 cut(s) 150, 201, 243, 1846
BstNI CCWGG 8 cut(s) 25, 40, 75, 441, 566, 1697, 1972, 2209
BstNSI RCATGY 1 cut(s) 289
BstPAI GACNNNNGTC 1 cut(s) 197
BstSFI CTRYAG 4 cut(s) 828, 1701, 1878, 1947
BstSLI GKGCMC 2 cut(s) 31, 211
BstUI CGCG 3 cut(s) 224, 239, 486
BstV1I GCAGC 6 cut(s) 1360, 1687, 1715, 1892, 1895, 2010
BstV2I GAAGAC 1 cut(s) 1406
BstX2I RGATCY 3 cut(s) 587, 920, 1232
BstXI CCANNNNNNTGG 2 cut(s) 74, 1475
BstYI RGATCY 3 cut(s) 587, 920, 1232
BstZI CGGCCG 1 cut(s) 147
Bsu36I CCTNAGG 2 cut(s) 32, 279
BsuI GTATCC 1 cut(s) 254
BsuRI GGCC 9 cut(s) 29, 37, 44, 149, 209, 600, 744, 1485, 1654
BtgI CCRYGG 4 cut(s) 102, 144, 745, 2067
BtsCI GGATG 5 cut(s) 110, 324, 517, 1606, 1869
BtsI GCAGTG 2 cut(s) 1940, 2242
BtsIMutI CAGTG 5 cut(s) 606, 1410, 1897, 1940, 2242
BtuMI TCGCGA 2 cut(s) 224, 486
Cac8I GCNNGC 8 cut(s) 18, 300, 432, 484, 1103, 1947, 2014, 2106
CaiI CAGNNNCTG 1 cut(s) 1733
CciI TCATGA 1 cut(s) 663
CfoI GCGC 2 cut(s) 425, 963
Cfr10I RCCGGY 2 cut(s) 314, 362
CseI GACGC 3 cut(s) 245, 1092, 1502
Csp6I GTAC 3 cut(s) 684, 1676, 2169
CviAII CATG 7 cut(s) 103, 286, 427, 664, 958, 1198, 1838
CviQI GTAC 3 cut(s) 684, 1676, 2169
DraI TTTAAA 3 cut(s) 862, 1221, 1455
EaeI YGGCCR 2 cut(s) 147, 598
EagI CGGCCG 1 cut(s) 147
Eam1104I CTCTTC 2 cut(s) 611, 1034
EarI CTCTTC 2 cut(s) 611, 1034
Ecl136II GAGCTC 1 cut(s) 124
EclXI CGGCCG 1 cut(s) 147
Eco130I CCWWGG 5 cut(s) 102, 329, 730, 1422, 1714
Eco147I AGGCCT 1 cut(s) 1654
Eco24I GRGCYC 5 cut(s) 31, 126, 165, 211, 838
Eco31I GGTCTC 2 cut(s) 887, 2129
Eco32I GATATC 2 cut(s) 820, 1005
Eco47I GGWCC 7 cut(s) 77, 192, 644, 727, 947, 1439, 2043
Eco52I CGGCCG 1 cut(s) 147
Eco53kI GAGCTC 1 cut(s) 124
Eco57I CTGAAG 2 cut(s) 1464, 1941
Eco81I CCTNAGG 2 cut(s) 32, 279
Eco88I CYCGRG 1 cut(s) 158
EcoICRI GAGCTC 1 cut(s) 124
EcoO109I RGGNCCY 8 cut(s) 27, 28, 42, 207, 727, 947, 1484, 2043
EcoRII CCWGG 8 cut(s) 23, 38, 73, 439, 564, 1695, 1970, 2207
EcoRV GATATC 2 cut(s) 820, 1005
EcoT14I CCWWGG 5 cut(s) 102, 329, 730, 1422, 1714
EcoT22I ATGCAT 1 cut(s) 1816
EcoT38I GRGCYC 5 cut(s) 31, 126, 165, 211, 838
ErhI CCWWGG 5 cut(s) 102, 329, 730, 1422, 1714
FaeI CATG 7 cut(s) 106, 289, 430, 667, 961, 1201, 1841
FaqI GGGAC 4 cut(s) 781, 933, 1988, 2056
FatI CATG 7 cut(s) 102, 285, 426, 663, 957, 1197, 1837
FauI CCCGC 2 cut(s) 175, 192
FauNDI CATATG 1 cut(s) 1909
FbaI TGATCA 1 cut(s) 541
FokI GGATG 5 cut(s) 97, 311, 504, 1613, 1856
FriOI GRGCYC 5 cut(s) 31, 126, 165, 211, 838
FspAI RTGCGCAY 1 cut(s) 962
FspBI CTAG 3 cut(s) 330, 1389, 1499
FspI TGCGCA 1 cut(s) 962
GlaI GCGC 2 cut(s) 424, 962
GsaI CCCAGC 4 cut(s) 157, 720, 1360, 1578
GsuI CTGGAG 1 cut(s) 548
HaeIII GGCC 9 cut(s) 29, 37, 44, 149, 209, 600, 744, 1485, 1654
HgaI GACGC 3 cut(s) 245, 1092, 1502
HhaI GCGC 2 cut(s) 425, 963
Hin1II CATG 7 cut(s) 106, 289, 430, 667, 961, 1201, 1841
Hin6I GCGC 2 cut(s) 423, 961
HinP1I GCGC 2 cut(s) 423, 961
HincII GTYRAC 2 cut(s) 322, 1789
HindII GTYRAC 2 cut(s) 322, 1789
HindIII AAGCTT 1 cut(s) 782
HpaI GTTAAC 1 cut(s) 1789
HphI GGTGA 4 cut(s) 393, 505, 1976, 2197
Hpy166II GTNNAC 7 cut(s) 144, 256, 322, 631, 797, 1765, 1789
Hpy188I TCNGA 7 cut(s) 592, 1009, 1186, 1735, 1810, 2005, 2145
Hpy8I GTNNAC 7 cut(s) 144, 256, 322, 631, 797, 1765, 1789
Hpy99I CGWCG 4 cut(s) 239, 389, 416, 1518
HpyAV CCTTC 8 cut(s) 91, 100, 472, 482, 1247, 1321, 1895, 1916
HpyCH4III ACNGT 7 cut(s) 661, 1034, 1307, 1405, 1960, 2038, 2068
HpyCH4IV ACGT 1 cut(s) 774
HpySE526I ACGT 1 cut(s) 774
Hsp92II CATG 7 cut(s) 106, 289, 430, 667, 961, 1201, 1841
HspAI GCGC 2 cut(s) 423, 961
KflI GGGWCCC 1 cut(s) 2043
Kpn2I TCCGGA 2 cut(s) 133, 1395
Ksp22I TGATCA 1 cut(s) 541
KspAI GTTAAC 1 cut(s) 1789
LmnI GCTCC 5 cut(s) 57, 68, 121, 136, 833
Lsp1109I GCAGC 6 cut(s) 1360, 1687, 1715, 1892, 1895, 2010
MaeI CTAG 3 cut(s) 330, 1389, 1499
MaeII ACGT 1 cut(s) 774
MaeIII GTNAC 3 cut(s) 106, 399, 904
MboII GAAGA 7 cut(s) 76, 628, 1051, 1166, 1274, 1411, 1996
MflI RGATCY 3 cut(s) 587, 920, 1232
MhlI GDGCHC 6 cut(s) 31, 126, 165, 211, 838, 1931
MlyI GAGTC 5 cut(s) 661, 1009, 1202, 1757, 2152
MmeI TCCRAC 3 cut(s) 898, 948, 1979
Mph1103I ATGCAT 1 cut(s) 1816
MroI TCCGGA 2 cut(s) 133, 1395
MroXI GAANNNNTTC 1 cut(s) 1261
MslI CAYNNNNRTG 2 cut(s) 290, 611
MspA1I CMGCKG 1 cut(s) 1180
MvaI CCWGG 8 cut(s) 25, 40, 75, 441, 566, 1697, 1972, 2209
MvnI CGCG 3 cut(s) 224, 239, 486
NciI CCSGG 6 cut(s) 119, 190, 197, 230, 1847, 2056
NcoI CCATGG 1 cut(s) 102
NdeI CATATG 1 cut(s) 1909
NlaIII CATG 7 cut(s) 106, 289, 430, 667, 961, 1201, 1841
NmeAIII GCCGAG 2 cut(s) 39, 1216
NmuCI GTSAC 2 cut(s) 106, 399
NruI TCGCGA 2 cut(s) 224, 486
NsbI TGCGCA 1 cut(s) 962
NsiI ATGCAT 1 cut(s) 1816
NspI RCATGY 1 cut(s) 289
NspV TTCGAA 1 cut(s) 931
PagI TCATGA 1 cut(s) 663
PasI CCCWGGG 1 cut(s) 39
PceI AGGCCT 1 cut(s) 1654
PcsI WCGNNNNNNNCGW 2 cut(s) 240, 249
PdmI GAANNNNTTC 1 cut(s) 1261
PfeI GAWTC 9 cut(s) 130, 213, 514, 603, 637, 1066, 1392, 1730, 2198
PfoI TCCNGGA 2 cut(s) 564, 2054
Ple19I CGATCG 1 cut(s) 243
PleI GAGTC 5 cut(s) 661, 1009, 1201, 1756, 2152
PpsI GAGTC 5 cut(s) 661, 1009, 1201, 1756, 2152
PpuMI RGGWCCY 3 cut(s) 727, 947, 2043
PshAI GACNNNNGTC 1 cut(s) 197
Psp124BI GAGCTC 1 cut(s) 126
Psp5II RGGWCCY 3 cut(s) 727, 947, 2043
Psp6I CCWGG 8 cut(s) 23, 38, 73, 439, 564, 1695, 1970, 2207
PspFI CCCAGC 4 cut(s) 153, 716, 1356, 1574
PspGI CCWGG 8 cut(s) 23, 38, 73, 439, 564, 1695, 1970, 2207
PspOMI GGGCCC 2 cut(s) 27, 207
PspPPI RGGWCCY 3 cut(s) 727, 947, 2043
PstI CTGCAG 3 cut(s) 1705, 1882, 1951
PstNI CAGNNNCTG 1 cut(s) 1733
PsuI RGATCY 3 cut(s) 587, 920, 1232
PvuI CGATCG 1 cut(s) 243
PvuII CAGCTG 1 cut(s) 1180
RruI TCGCGA 2 cut(s) 224, 486
RsaI GTAC 3 cut(s) 685, 1677, 2170
RsaNI GTAC 3 cut(s) 684, 1676, 2169
RseI CAYNNNNRTG 2 cut(s) 290, 611
SacI GAGCTC 1 cut(s) 126
SchI GAGTC 5 cut(s) 661, 1009, 1202, 1757, 2152
SduI GDGCHC 6 cut(s) 31, 126, 165, 211, 838, 1931
SfcI CTRYAG 4 cut(s) 828, 1701, 1878, 1947
SfuI TTCGAA 1 cut(s) 931
SgrAI CRCCGGYG 1 cut(s) 314
SinI GGWCC 7 cut(s) 77, 192, 644, 727, 947, 1439, 2043
SmiMI CAYNNNNRTG 2 cut(s) 290, 611
SmlI CTYRAG 3 cut(s) 46, 125, 1322
SmoI CTYRAG 3 cut(s) 46, 125, 1322
SseBI AGGCCT 1 cut(s) 1654
SsiI CCGC 6 cut(s) 150, 168, 171, 174, 185, 1692
SspMI CTAG 3 cut(s) 330, 1389, 1499
SstI GAGCTC 1 cut(s) 126
StuI AGGCCT 1 cut(s) 1654
StyI CCWWGG 5 cut(s) 102, 329, 730, 1422, 1714
TaaI ACNGT 7 cut(s) 661, 1034, 1307, 1405, 1960, 2038, 2068
TaiI ACGT 1 cut(s) 777
TaqI TCGA 6 cut(s) 243, 387, 931, 1018, 1285, 2123
TaqII GACCGA 1 cut(s) 2210
TatI WGTACW 2 cut(s) 683, 1675
TauI GCSGC 4 cut(s) 152, 173, 176, 1694
TfiI GAWTC 9 cut(s) 130, 213, 514, 603, 637, 1066, 1392, 1730, 2198
TscAI CASTG 5 cut(s) 613, 1410, 1897, 1947, 2242
TseFI GTSAC 2 cut(s) 106, 399
TseI GCWGC 6 cut(s) 1373, 1700, 1703, 1880, 1883, 2023
Tsp45I GTSAC 2 cut(s) 106, 399
TspDTI ATGAA 6 cut(s) 461, 527, 629, 1316, 1476, 2106
TspGWI ACGGA 3 cut(s) 273, 280, 1982
TspRI CASTG 5 cut(s) 613, 1410, 1897, 1947, 2242
VpaK11BI GGWCC 7 cut(s) 77, 192, 644, 727, 947, 1439, 2043
XapI RAATTY 1 cut(s) 1757
XbaI TCTAGA 1 cut(s) 1498
XceI RCATGY 1 cut(s) 289
XcmI CCANNNNNNNNNTGG 2 cut(s) 1424, 2146
XmaJI CCTAGG 1 cut(s) 329
XmnI GAANNNNTTC 1 cut(s) 1261
XspI CTAG 3 cut(s) 330, 1389, 1499
Zsp2I ATGCAT 1 cut(s) 1816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.