Rroxscaffold_6G00392660

Belongs to the complex I 75 kDa subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0011004
Physical Location & Seq
Forward (+)
12319680 .. 12326576
6897 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00392660.1

Sequence Viewer

Length: 2256 bp
ATGGGGTTGGGATCGCTTGCTTCCAGGGCCCTCAGGCCCAGGGCCGCAAGGCTCCTCCTCGGCTCCCAAAACCCTGGACCCTTCCTCTCCTTCCAGAGAACCATGGTCACATCCACCCCGGAGCTCAAGAATCCCGAGCCGTCCACGGCCGCCCAGCCCGAGCCCCCCGCCGCCGACCTCCCGCCCCGGACCCCGGTCGGTGGGGCCCGAATCCACTTCGCGAACCCGGACGATGCGATCGAGGTGTTCGTGGACGGATACCCTGTGAAAATCCCTAAGGGCATGTCCGTGTTGCAAGCGTGTGAGGTCGCCGGTGTTGACATCCCTAGGTTTTGCTACCACAGCAGATTGTCCATTGCCGGTAATTGCCGTATGTGTCTCGTCGAGGTTGAGAAGTCACCCAAGCCCGTCGCCTCTTGCGCCATGCCTGCTATGCCTGGTATGAAAATTAAGACTGACACTCCAATAGCCAAGAAGGCTCGCGAAGGGGTGATGGAGTTTCTGTTGATGAATCATCCTTTGGATTGTCCAATTTGTGATCAGGGTGGAGAATGTGATCTCCAGGATCAGTCTATGGCATTTGGATCTGATCGTGGCCGATTCACTGAAATGAAGAGATCAGTTGTCGACAAGAATCTTGGCCCTTTGGTAAAGACTGTCATGACTCGCTGTATTCAATGTACAAGGTGCGTTAGGTTTGCAACAGAGGTCGCTGGGGTTCAGGACCTTGGAATGTTAGGCCGTGGAAGTGGAGAGGAAATTGGGACTTACGTTGAAAAGCTTATGACAAGTGAACTTTCAGGAAATGTTATAGATATCTGTCCTGTAGGAGCCCTAACCTCAAAACCATTTGCATTTAAAGCTCGTAATTGGGAATTAAAGGGAACAGAGACCATTGATGTTACTGATGCTGTTGGATCCAACATTCGAATTGATAGCAGAGGTCCAGAAGTCATGCGCATTGTTCCACGCTTAAATGAGGACATAAATGAGGAATGGATATCAGACAAGACTCGATTTTGTTATGACGGTTTGAAGAGGCAAAGGTTGAATGACCCTATGATTCGTGGTGCTGATGGACGCTTTAAGCCCGTGAGCTGGCGTGATGCCCTTGCTGTTGTTGCTGAGATGGCTCATAAAGTTAAGCCAGAAGAAATTGTTGGGGTTGCTGGTCAGCTGTCTGATGCCGAGTCCATGATGGCACTAAAAGACTTTTTAAATAAAATGGGGTCTAACAATGTGTGGTGCGAAGGAAATGGTTCCAATCATGATGCTGATCTGCGATCTGGATATATAATGAACAGTAGCATTGCTGGACTTGAGAAGGCAGATGCTTTTCTATTGGTTGGCACCCAGCCAAGAGTAGAAGCTGCTATTGTAAATGCTAGAATCCGGAAGACAGTGTTATCTAACCACGCCAAGGTTGGGTACGTTGGTCCTGAAGCTGATTTAAACTATGAACACCAGCATCTTGGAACAGGCCCTGATACACTGCTAGAACTTGCTGAGCGTCGCCACCCCTTTTCCTCAGTCCTCTTAAATGCCAAAAACCCTGCCATTATTGTTGGTGCTGGGCTGTTTGAGAGAAAAGACAAGGATGCAATTTTCTCGGCCCTTGAAACCATTGCCAAATATGCTGATGCTGTAAGGCCTGATTGGAATGGATTTAATGTACTGCTTCTCAAAGCCGCCCAGGCTGCAGCACTTGACCTTGGACTTGTGCCAGAATCTGAAAACAGCATTGAGTCTGCAAAATTTGTTTACCTGATGGGTGCTGATGATGTTAACTTAGATAAGGTTCCATCTGATGCATTTGTGGTTTATCAAGGGCATCATGGTGACCGGGGTGTGTATCGGGCTAATGTCATCCTCCCTGCAGCAGCGTTCAGTGAAAAGGAAGGAACATATGTAAATACTGAAGGGTGCTCTCAGCAAACATTGCCTGCAGTTCCAACTGTCGGTGATGCCAGGGACGACTGGAAGATACTCCGCGCTTTATCTGAGGTAGCAGGCGTGAAGCTGCCTTACGATACGGTTGGGACCCTCCGATCCCGGATAAAGACCGTGGCACCAAACATCTTAAACATAGACGAGAGAGAGCCAGCTACTTTTTCATTTTCGATTAAGCCTGAGACCACTCAGAAAATGGATTCAACTCCATTTGGTACTGCTGTTGATAATTTCTATATGACCGATTCCATCACCAGGGCATCAAAGATAATGGCACAATGCAGTGCGTTGCTATTGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko04723 ko04932 ko05010 ko05012 ko05016 map00190 map01100 map04714 map04723 map04932 map05010 map05012 map05016
Pfam Domains
Protein Families
Fer2_4 Molybdopterin NADH-G_4Fe-4S_3 NADH_dhqG_C

Protein Analysis

751

Amino Acids

81.69

Weight (kDa)

6.1

Isoelectric Point (pI)

35.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer2_4 PF13510 78 - 154 6.4e-19 2Fe-2S iron-sulfur cluster binding domain
Fer2 PF00111 83 - 132 7.8e-07 2Fe-2S iron-sulfur cluster binding domain
NADH-G_4Fe-4S_3 PF10588 161 - 198 1.4e-16 NADH-ubiquinone oxidoreductase-G iron-sulfur binding region
Fer4_Nqo3 PF22117 214 - 280 4.8e-33 NADH-quinone oxidoreductase subunit 3, ferredoxin-like domain
Fer4_NDSU1 PF22151 293 - 343 5.7e-30 NADH-ubiquinone oxidoreductase NDSU1/NuoG-like, 4Fe-4S domain
Molybdopterin PF00384 349 - 669 2e-109 Molybdopterin oxidoreductase
NADH_dhqG_C PF09326 709 - 744 1.7e-12 NADH-ubiquinone oxidoreductase subunit G, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011004)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37510 AT5G37510
fragaria_vesca FvH4_1g30070
malus_domestica MD09G1268900.v1.1 MD17G1267300.v1.1
prunus_persica Prupe.3G165400_v2.0.a1
pyrus_communis pycom09g18080 pycom17g26640
rosa_chinensis RchiOBHm_Chr3g0491991
rosa_laevigata RLG00000022799 RLG00000022807
rosa_multiflora Rmu_sc0005238.1_g000005 Rmu_sc0036946.1_g000001
rosa_roxburghii Rroxscaffold_6G00392660
rosa_rugosa Rorug03G0251000
rosa_samantha Rh3AG301000 Rh3BG337200 Rh3CG334300 Rh3DG336600
rosa_wichuraiana Rw3G026610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 959
AccB1I GGYRCC 2 cut(s) 1349, 2068
AccI GTMKAC 1 cut(s) 627
AccII CGCG 3 cut(s) 221, 483, 1992
AccIII TCCGGA 1 cut(s) 1392
AciI CCGC 7 cut(s) 45, 150, 168, 171, 182, 1689, 1990
AclWI GGATC 6 cut(s) 19, 573, 592, 912, 925, 2043
AcoI YGGCCR 2 cut(s) 147, 595
AcsI RAATTY 1 cut(s) 1754
AcuI CTGAAG 2 cut(s) 1461, 1938
AfaI GTAC 4 cut(s) 682, 1430, 1674, 2167
AfiI CCNNNNNNNGG 7 cut(s) 193, 200, 1098, 1420, 1425, 1958, 2205
AgsI TTSAA 7 cut(s) 677, 776, 1036, 1051, 1619, 2154, 2248
AjnI CCWGG 8 cut(s) 23, 38, 73, 436, 561, 1692, 1967, 2204
AjuI GAANNNNNNNTTGG 4 cut(s) 503, 535, 1143, 1175
AluBI AGCT 9 cut(s) 124, 781, 863, 1098, 1177, 1370, 1445, 2020, 2105
AluI AGCT 9 cut(s) 124, 781, 863, 1098, 1177, 1370, 1445, 2020, 2105
Alw21I GWGCWC 2 cut(s) 126, 1928
Alw26I GTCTC 3 cut(s) 383, 884, 2126
AlwI GGATC 6 cut(s) 19, 573, 592, 912, 925, 2043
AlwNI CAGNNNCTG 2 cut(s) 1484, 1730
Ama87I CYCGRG 2 cut(s) 134, 158
Aor13HI TCCGGA 1 cut(s) 1392
ApaI GGGCCC 2 cut(s) 31, 208
ApeKI GCWGC 6 cut(s) 1370, 1697, 1700, 1877, 1880, 2020
ApoI RAATTY 1 cut(s) 1754
Asp700I GAANNNNTTC 1 cut(s) 1258
AspA2I CCTAGG 1 cut(s) 326
AspLEI GCGC 3 cut(s) 422, 960, 1994
AsuC2I CCSGG 6 cut(s) 119, 187, 194, 227, 1844, 2053
AsuHPI GGTGA 5 cut(s) 390, 502, 1850, 1973, 2194
AsuII TTCGAA 1 cut(s) 928
AvaI CYCGRG 2 cut(s) 134, 158
AvaII GGWCC 6 cut(s) 77, 189, 724, 944, 1436, 2040
AvrII CCTAGG 1 cut(s) 326
AxyI CCTNAGG 2 cut(s) 32, 276
BaeGI GKGCMC 2 cut(s) 31, 208
BaeI ACNNNNGTAYC 4 cut(s) 1420, 1453, 2157, 2190
BamHI GGATCC 1 cut(s) 917
BanI GGYRCC 2 cut(s) 1349, 2068
BanII GRGCYC 5 cut(s) 31, 126, 165, 208, 835
BarI GAAGNNNNNNTAC 2 cut(s) 2009, 2041
BbsI GAAGAC 1 cut(s) 1403
Bbv12I GWGCWC 2 cut(s) 126, 1928
BbvI GCAGC 6 cut(s) 1357, 1684, 1712, 1889, 1892, 2007
BccI CCATC 7 cut(s) 487, 1070, 1123, 1192, 1762, 1810, 2207
BceAI ACGGC 4 cut(s) 124, 162, 354, 726
BcgI CGANNNNNNTGC 2 cut(s) 1590, 1624
BciT130I CCWGG 8 cut(s) 25, 40, 75, 438, 563, 1694, 1969, 2206
BciVI GTATCC 1 cut(s) 251
BclI TGATCA 1 cut(s) 538
BcnI CCSGG 6 cut(s) 119, 187, 194, 227, 1844, 2053
BcoDI GTCTC 3 cut(s) 383, 884, 2126
BfaI CTAG 3 cut(s) 327, 1386, 1496
BfmI CTRYAG 4 cut(s) 825, 1698, 1875, 1944
BfuI GTATCC 1 cut(s) 251
BglI GCCNNNNNGGC 2 cut(s) 476, 1694
BlnI CCTAGG 1 cut(s) 326
BlpI GCTNAGC 1 cut(s) 1506
Bme18I GGWCC 6 cut(s) 77, 189, 724, 944, 1436, 2040
BmeT110I CYCGRG 2 cut(s) 134, 158
BoxI GACNNNNGTC 1 cut(s) 194
BpiI GAAGAC 1 cut(s) 1403
BpmI CTGGAG 1 cut(s) 545
Bpu1102I GCTNAGC 1 cut(s) 1506
Bpu14I TTCGAA 1 cut(s) 928
BpuEI CTTGAG 2 cut(s) 110, 1340
BpuMI CCSGG 6 cut(s) 119, 187, 194, 227, 1844, 2053
BsaBI GATNNNNATC 1 cut(s) 1275
BsaI GGTCTC 2 cut(s) 884, 2126
BsaWI WCCGGW 1 cut(s) 1392
BsaXI ACNNNNNCTCC 4 cut(s) 445, 475, 488, 518
Bsc4I CCNNNNNNNGG 7 cut(s) 193, 200, 1098, 1420, 1425, 1958, 2205
Bse118I RCCGGY 2 cut(s) 311, 359
Bse1I ACTGG 1 cut(s) 1982
Bse21I CCTNAGG 2 cut(s) 32, 276
Bse3DI GCAATG 4 cut(s) 354, 1308, 1623, 1937
Bse8I GATNNNNATC 1 cut(s) 1275
BseAI TCCGGA 1 cut(s) 1392
BseBI CCWGG 8 cut(s) 25, 40, 75, 438, 563, 1694, 1969, 2206
BseGI GGATG 5 cut(s) 110, 321, 514, 1603, 1866
BseJI GATNNNNATC 1 cut(s) 1275
BseLI CCNNNNNNNGG 7 cut(s) 193, 200, 1098, 1420, 1425, 1958, 2205
BseMI GCAATG 4 cut(s) 354, 1308, 1623, 1937
BseMII CTCAG 8 cut(s) 46, 1116, 1497, 1542, 1943, 1992, 2121, 2153
BseNI ACTGG 1 cut(s) 1982
BseRI GAGGAG 2 cut(s) 44, 47
BseSI GKGCMC 2 cut(s) 31, 208
BseX3I CGGCCG 1 cut(s) 147
BseXI GCAGC 6 cut(s) 1357, 1684, 1712, 1889, 1892, 2007
BseYI CCCAGC 4 cut(s) 153, 713, 1353, 1571
Bsh1236I CGCG 3 cut(s) 221, 483, 1992
Bsh1285I CGRYCG 3 cut(s) 150, 198, 240
BshNI GGYRCC 2 cut(s) 1349, 2068
BsiEI CGRYCG 3 cut(s) 150, 198, 240
BsiHKAI GWGCWC 2 cut(s) 126, 1928
BsiHKCI CYCGRG 2 cut(s) 134, 158
BsiSI CCGG 9 cut(s) 119, 187, 194, 227, 312, 360, 1393, 1843, 2053
BslFI GGGAC 3 cut(s) 778, 1985, 2053
BslI CCNNNNNNNGG 7 cut(s) 193, 200, 1098, 1420, 1425, 1958, 2205
BsmAI GTCTC 3 cut(s) 383, 884, 2126
BsmFI GGGAC 3 cut(s) 778, 1985, 2053
Bso31I GGTCTC 2 cut(s) 884, 2126
BsoBI CYCGRG 2 cut(s) 134, 158
Bsp119I TTCGAA 1 cut(s) 928
Bsp120I GGGCCC 2 cut(s) 27, 204
Bsp1286I GDGCHC 6 cut(s) 31, 126, 165, 208, 835, 1928
Bsp13I TCCGGA 1 cut(s) 1392
Bsp1407I TGTACA 1 cut(s) 680
Bsp1720I GCTNAGC 1 cut(s) 1506
Bsp19I CCATGG 1 cut(s) 102
Bsp68I TCGCGA 2 cut(s) 221, 483
BspACI CCGC 7 cut(s) 45, 150, 168, 171, 182, 1689, 1990
BspCNI CTCAG 8 cut(s) 45, 1117, 1498, 1541, 1942, 1993, 2122, 2152
BspEI TCCGGA 1 cut(s) 1392
BspFNI CGCG 3 cut(s) 221, 483, 1992
BspHI TCATGA 2 cut(s) 660, 1267
BspMAI CTGCAG 3 cut(s) 1702, 1879, 1948
BspPI GGATC 6 cut(s) 19, 573, 592, 912, 925, 2043
BspT104I TTCGAA 1 cut(s) 928
BspT107I GGYRCC 2 cut(s) 1349, 2068
BspTNI GGTCTC 2 cut(s) 884, 2126
BsrDI GCAATG 4 cut(s) 354, 1308, 1623, 1937
BsrFI RCCGGY 2 cut(s) 311, 359
BsrGI TGTACA 1 cut(s) 680
BsrI ACTGG 1 cut(s) 1982
BssAI RCCGGY 2 cut(s) 311, 359
BssT1I CCWWGG 5 cut(s) 102, 326, 727, 1419, 1711
Bst2UI CCWGG 8 cut(s) 25, 40, 75, 438, 563, 1694, 1969, 2206
Bst4CI ACNGT 7 cut(s) 658, 1031, 1304, 1402, 1957, 2035, 2065
Bst6I CTCTTC 2 cut(s) 608, 1031
BstAPI GCANNNNNTGC 1 cut(s) 1939
BstAUI TGTACA 1 cut(s) 680
BstBI TTCGAA 1 cut(s) 928
BstC8I GCNNGC 8 cut(s) 18, 297, 429, 481, 1100, 1944, 2011, 2103
BstDSI CCRYGG 4 cut(s) 102, 144, 742, 2064
BstEII GGTNACC 1 cut(s) 1838
BstF5I GGATG 5 cut(s) 110, 321, 514, 1603, 1866
BstFNI CGCG 3 cut(s) 221, 483, 1992
BstHHI GCGC 3 cut(s) 422, 960, 1994
BstMAI GTCTC 3 cut(s) 383, 884, 2126
BstMCI CGRYCG 3 cut(s) 150, 198, 240
BstNI CCWGG 8 cut(s) 25, 40, 75, 438, 563, 1694, 1969, 2206
BstNSI RCATGY 1 cut(s) 286
BstPAI GACNNNNGTC 1 cut(s) 194
BstPI GGTNACC 1 cut(s) 1838
BstSFI CTRYAG 4 cut(s) 825, 1698, 1875, 1944
BstSLI GKGCMC 2 cut(s) 31, 208
BstUI CGCG 3 cut(s) 221, 483, 1992
BstV1I GCAGC 6 cut(s) 1357, 1684, 1712, 1889, 1892, 2007
BstV2I GAAGAC 1 cut(s) 1403
BstX2I RGATCY 2 cut(s) 584, 917
BstXI CCANNNNNNTGG 2 cut(s) 74, 1472
BstYI RGATCY 2 cut(s) 584, 917
BstZI CGGCCG 1 cut(s) 147
Bsu36I CCTNAGG 2 cut(s) 32, 276
BsuI GTATCC 1 cut(s) 251
BtgI CCRYGG 4 cut(s) 102, 144, 742, 2064
BtsCI GGATG 5 cut(s) 110, 321, 514, 1603, 1866
BtsI GCAGTG 2 cut(s) 1490, 2239
BtsIMutI CAGTG 5 cut(s) 603, 1407, 1490, 1894, 2239
BtuMI TCGCGA 2 cut(s) 221, 483
Cac8I GCNNGC 8 cut(s) 18, 297, 429, 481, 1100, 1944, 2011, 2103
CaiI CAGNNNCTG 2 cut(s) 1484, 1730
CciI TCATGA 2 cut(s) 660, 1267
CfoI GCGC 3 cut(s) 422, 960, 1994
Cfr10I RCCGGY 2 cut(s) 311, 359
CseI GACGC 2 cut(s) 1089, 1499
Csp6I GTAC 4 cut(s) 681, 1429, 1673, 2166
CviAII CATG 8 cut(s) 103, 283, 424, 661, 955, 1195, 1268, 1835
CviQI GTAC 4 cut(s) 681, 1429, 1673, 2166
DraI TTTAAA 3 cut(s) 859, 1218, 1452
EaeI YGGCCR 2 cut(s) 147, 595
EagI CGGCCG 1 cut(s) 147
Eam1104I CTCTTC 2 cut(s) 608, 1031
EarI CTCTTC 2 cut(s) 608, 1031
Ecl136II GAGCTC 1 cut(s) 124
EclXI CGGCCG 1 cut(s) 147
Eco130I CCWWGG 5 cut(s) 102, 326, 727, 1419, 1711
Eco147I AGGCCT 1 cut(s) 1651
Eco24I GRGCYC 5 cut(s) 31, 126, 165, 208, 835
Eco31I GGTCTC 2 cut(s) 884, 2126
Eco32I GATATC 2 cut(s) 817, 1002
Eco47I GGWCC 6 cut(s) 77, 189, 724, 944, 1436, 2040
Eco52I CGGCCG 1 cut(s) 147
Eco53kI GAGCTC 1 cut(s) 124
Eco57I CTGAAG 2 cut(s) 1461, 1938
Eco81I CCTNAGG 2 cut(s) 32, 276
Eco88I CYCGRG 2 cut(s) 134, 158
Eco91I GGTNACC 1 cut(s) 1838
EcoICRI GAGCTC 1 cut(s) 124
EcoO109I RGGNCCY 6 cut(s) 27, 28, 204, 724, 1481, 2040
EcoO65I GGTNACC 1 cut(s) 1838
EcoRII CCWGG 8 cut(s) 23, 38, 73, 436, 561, 1692, 1967, 2204
EcoRV GATATC 2 cut(s) 817, 1002
EcoT14I CCWWGG 5 cut(s) 102, 326, 727, 1419, 1711
EcoT22I ATGCAT 1 cut(s) 1813
EcoT38I GRGCYC 5 cut(s) 31, 126, 165, 208, 835
ErhI CCWWGG 5 cut(s) 102, 326, 727, 1419, 1711
FaeI CATG 8 cut(s) 106, 286, 427, 664, 958, 1198, 1271, 1838
FaqI GGGAC 3 cut(s) 778, 1985, 2053
FatI CATG 8 cut(s) 102, 282, 423, 660, 954, 1194, 1267, 1834
FauI CCCGC 2 cut(s) 175, 189
FauNDI CATATG 1 cut(s) 1906
FbaI TGATCA 1 cut(s) 538
FblI GTMKAC 1 cut(s) 627
FokI GGATG 5 cut(s) 97, 308, 501, 1610, 1853
FriOI GRGCYC 5 cut(s) 31, 126, 165, 208, 835
FspAI RTGCGCAY 1 cut(s) 959
FspBI CTAG 3 cut(s) 327, 1386, 1496
FspI TGCGCA 1 cut(s) 959
GlaI GCGC 3 cut(s) 421, 959, 1993
GsaI CCCAGC 4 cut(s) 157, 717, 1357, 1575
GsuI CTGGAG 1 cut(s) 545
HapII CCGG 9 cut(s) 119, 187, 194, 227, 312, 360, 1393, 1843, 2053
HgaI GACGC 2 cut(s) 1089, 1499
HhaI GCGC 3 cut(s) 422, 960, 1994
Hin1II CATG 8 cut(s) 106, 286, 427, 664, 958, 1198, 1271, 1838
Hin6I GCGC 3 cut(s) 420, 958, 1992
HinP1I GCGC 3 cut(s) 420, 958, 1992
HincII GTYRAC 3 cut(s) 319, 628, 1786
HindII GTYRAC 3 cut(s) 319, 628, 1786
HindIII AAGCTT 1 cut(s) 779
HpaI GTTAAC 1 cut(s) 1786
HpaII CCGG 9 cut(s) 119, 187, 194, 227, 312, 360, 1393, 1843, 2053
HphI GGTGA 5 cut(s) 390, 502, 1850, 1973, 2194
Hpy166II GTNNAC 7 cut(s) 144, 253, 319, 628, 794, 1762, 1786
Hpy188I TCNGA 8 cut(s) 589, 1006, 1183, 1732, 1807, 2002, 2048, 2142
Hpy8I GTNNAC 7 cut(s) 144, 253, 319, 628, 794, 1762, 1786
Hpy99I CGWCG 3 cut(s) 386, 413, 1515
HpyAV CCTTC 8 cut(s) 91, 100, 469, 479, 1244, 1318, 1892, 1913
HpyCH4III ACNGT 7 cut(s) 658, 1031, 1304, 1402, 1957, 2035, 2065
HpyCH4IV ACGT 2 cut(s) 771, 1431
HpySE526I ACGT 2 cut(s) 771, 1431
Hsp92II CATG 8 cut(s) 106, 286, 427, 664, 958, 1198, 1271, 1838
HspAI GCGC 3 cut(s) 420, 958, 1992
KflI GGGWCCC 1 cut(s) 2040
Kpn2I TCCGGA 1 cut(s) 1392
Ksp22I TGATCA 1 cut(s) 538
KspAI GTTAAC 1 cut(s) 1786
LmnI GCTCC 4 cut(s) 57, 68, 121, 830
Lsp1109I GCAGC 6 cut(s) 1357, 1684, 1712, 1889, 1892, 2007
MaeI CTAG 3 cut(s) 327, 1386, 1496
MaeII ACGT 2 cut(s) 771, 1431
MaeIII GTNAC 4 cut(s) 106, 396, 901, 1838
MboII GAAGA 5 cut(s) 625, 1048, 1163, 1408, 1993
MflI RGATCY 2 cut(s) 584, 917
MhlI GDGCHC 6 cut(s) 31, 126, 165, 208, 835, 1928
MlyI GAGTC 4 cut(s) 658, 1006, 1199, 1754
MmeI TCCRAC 3 cut(s) 895, 945, 1976
Mph1103I ATGCAT 1 cut(s) 1813
MroI TCCGGA 1 cut(s) 1392
MroXI GAANNNNTTC 1 cut(s) 1258
MslI CAYNNNNRTG 3 cut(s) 287, 608, 1836
MspA1I CMGCKG 1 cut(s) 1177
MspI CCGG 9 cut(s) 119, 187, 194, 227, 312, 360, 1393, 1843, 2053
MvaI CCWGG 8 cut(s) 25, 40, 75, 438, 563, 1694, 1969, 2206
MvnI CGCG 3 cut(s) 221, 483, 1992
NciI CCSGG 6 cut(s) 119, 187, 194, 227, 1844, 2053
NcoI CCATGG 1 cut(s) 102
NdeI CATATG 1 cut(s) 1906
NlaIII CATG 8 cut(s) 106, 286, 427, 664, 958, 1198, 1271, 1838
NmeAIII GCCGAG 3 cut(s) 39, 1213, 1589
NmuCI GTSAC 3 cut(s) 106, 396, 1838
NruI TCGCGA 2 cut(s) 221, 483
NsbI TGCGCA 1 cut(s) 959
NsiI ATGCAT 1 cut(s) 1813
NspI RCATGY 1 cut(s) 286
NspV TTCGAA 1 cut(s) 928
PagI TCATGA 2 cut(s) 660, 1267
PasI CCCWGGG 1 cut(s) 39
PceI AGGCCT 1 cut(s) 1651
PcsI WCGNNNNNNNCGW 2 cut(s) 237, 246
PdmI GAANNNNTTC 1 cut(s) 1258
PfoI TCCNGGA 2 cut(s) 561, 2051
Ple19I CGATCG 1 cut(s) 240
PleI GAGTC 4 cut(s) 658, 1006, 1198, 1753
PpsI GAGTC 4 cut(s) 658, 1006, 1198, 1753
PpuMI RGGWCCY 2 cut(s) 724, 2040
PshAI GACNNNNGTC 1 cut(s) 194
Psp124BI GAGCTC 1 cut(s) 126
Psp5II RGGWCCY 2 cut(s) 724, 2040
Psp6I CCWGG 8 cut(s) 23, 38, 73, 436, 561, 1692, 1967, 2204
PspEI GGTNACC 1 cut(s) 1838
PspFI CCCAGC 4 cut(s) 153, 713, 1353, 1571
PspGI CCWGG 8 cut(s) 23, 38, 73, 436, 561, 1692, 1967, 2204
PspOMI GGGCCC 2 cut(s) 27, 204
PspPPI RGGWCCY 2 cut(s) 724, 2040
PstI CTGCAG 3 cut(s) 1702, 1879, 1948
PstNI CAGNNNCTG 2 cut(s) 1484, 1730
PsuI RGATCY 2 cut(s) 584, 917
PvuI CGATCG 1 cut(s) 240
PvuII CAGCTG 1 cut(s) 1177
RruI TCGCGA 2 cut(s) 221, 483
RsaI GTAC 4 cut(s) 682, 1430, 1674, 2167
RsaNI GTAC 4 cut(s) 681, 1429, 1673, 2166
RseI CAYNNNNRTG 3 cut(s) 287, 608, 1836
SacI GAGCTC 1 cut(s) 126
SalI GTCGAC 1 cut(s) 626
SchI GAGTC 4 cut(s) 658, 1006, 1199, 1754
SduI GDGCHC 6 cut(s) 31, 126, 165, 208, 835, 1928
SfcI CTRYAG 4 cut(s) 825, 1698, 1875, 1944
SfuI TTCGAA 1 cut(s) 928
SgrAI CRCCGGYG 1 cut(s) 311
SinI GGWCC 6 cut(s) 77, 189, 724, 944, 1436, 2040
SmiMI CAYNNNNRTG 3 cut(s) 287, 608, 1836
SmlI CTYRAG 2 cut(s) 125, 1319
SmoI CTYRAG 2 cut(s) 125, 1319
SseBI AGGCCT 1 cut(s) 1651
SsiI CCGC 7 cut(s) 45, 150, 168, 171, 182, 1689, 1990
SspMI CTAG 3 cut(s) 327, 1386, 1496
SstI GAGCTC 1 cut(s) 126
StuI AGGCCT 1 cut(s) 1651
StyI CCWWGG 5 cut(s) 102, 326, 727, 1419, 1711
TaaI ACNGT 7 cut(s) 658, 1031, 1304, 1402, 1957, 2035, 2065
TaiI ACGT 2 cut(s) 774, 1434
TaqI TCGA 6 cut(s) 240, 384, 627, 928, 1015, 2120
TaqII GACCGA 1 cut(s) 2207
TatI WGTACW 2 cut(s) 680, 1672
TauI GCSGC 4 cut(s) 47, 152, 173, 1691
TscAI CASTG 5 cut(s) 610, 1407, 1497, 1894, 2239
TseFI GTSAC 3 cut(s) 106, 396, 1838
TseI GCWGC 6 cut(s) 1370, 1697, 1700, 1877, 1880, 2020
Tsp45I GTSAC 3 cut(s) 106, 396, 1838
TspDTI ATGAA 6 cut(s) 458, 524, 626, 1313, 1473, 2103
TspGWI ACGGA 2 cut(s) 270, 277
TspRI CASTG 5 cut(s) 610, 1407, 1497, 1894, 2239
VpaK11BI GGWCC 6 cut(s) 77, 189, 724, 944, 1436, 2040
XapI RAATTY 1 cut(s) 1754
XceI RCATGY 1 cut(s) 286
XcmI CCANNNNNNNNNTGG 2 cut(s) 1421, 2143
XmaJI CCTAGG 1 cut(s) 326
XmiI GTMKAC 1 cut(s) 627
XmnI GAANNNNTTC 1 cut(s) 1258
XspI CTAG 3 cut(s) 327, 1386, 1496
Zsp2I ATGCAT 1 cut(s) 1813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.