FvH4_2g00340

Belongs to the glycosyltransferase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0006699
Physical Location & Seq
Reverse (-)
424032 .. 430146
6115 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g00340.t1

Sequence Viewer

Length: 3081 bp
ATGGCGGGAAACGACTGGTTAAACGGTTACCTGGAGGCGATTCTGGATGCAGGAAGTAAGAGTAACACAAAGAAGGGGAGCGATGGGAAGCAGAAGATTGCCAAGTTTGAGCAGCAAGTCAAGGAGGAGAAGTTGTTTAGTCCCACAAAATACTTTGTGGAAGAAGTAGTTAACAGCTTTGATGAGTCTGACCTCCACAGGACATGGGTTAAGGTAATAGCTACGAGGAACACTCGTGAACGCAGCAATAGGCTTGAGAATATGTGCTGGCGCATTTGGCATCTCGCTCGTAAGAAGAAACAGATAGCATGGGATGATGCTCGCAGACTTGCACGGCGCCGTCTGGAGAGGGAGAAAGGCCGCCATGACGCTGCAGAGGACCTTTCCGAGCTGTCTGAAGGGGAGAAGGAAAAAGGTGAGACCAACTTTATAGAACCGCCTGTCAAGGAAATAGCAAGAATCAACTCAGAAATGCGATTGTGGTCTGAGGATGACAACAGGACTAGGCATCTTTACATTGTCTTAATCAGTATGCATGGGTTGGTCCGTGGAGAAAACATGGAACTGGGAAGAGATTCTGATACAGGTGGTCAGGTGAAGTATGTAGTAGAACTTGCTCGTGCTCTGGCCAATACAAAGGGAGTCTATAGAGTTGATCTGTTAACAAGGCAAATCACTTCACCGGAGGTAGACTACAGCTACGGTGAACCTAATGAGATGCTCATTTGCCCGCCTGACGGCGGCGGCAGCTGCGGAGCCTACATTATCCGTCTTCCATGTGGACCTCGCGACAAGTACATACCGAAAGAGTCACTATGGCCTCACATACCTGAATTTATTGATGGGGCTCTCGGCCACATTGTGAACATGGCAAGAGCTCTAGGCGAAGAGGTCAATGGTGGGAAACCAACATGGCCTTATGTTATCCACGGCCACTATGCTGATGCTGGGGAAGTGGCTGCTCAGTTGTCTGGTGCCTTGAATGTCCCCATGGTGCTGACCGGGCATTCCCTAGGAAGAAACAAGTTTGAACAACTGCTCAAACAGGGAAGATTGTCGAAGGAGGACATCAATGGAACCTACAAGATTATGAAGAGGATCGAGGCTGAAGAGCTGGGTTTGGATGCTGCTGAAATGGTGGTGACTAGTACTAGGCAAGAGATTGAAGAGCAGTGGGGACTGTATGATGGGTTTGATCTCAAGTTGGAGAGGAAGCTCAGGGTTAGGAGACGCCGGGGAGTTAGTTGCCTTGGACGATACATGCCAAGAATGGTGGTAATACCGCCAGGCATGGACTTCAGCTATGTTACAGTGCAAGAAGCTGAAGGAGATGGAGATCTCAAATCCCTGCTTGGTTCTGACAGATCTCAAAGGAAAAGGAATCTCCCTCCAATATGGTCTGAGGTAATGAGATTTTTTACTAATCCTCACAAGCCGACTATACTTGCATTGTCTCGTCCTGACCCAAAGAAAAATGTTACTACATTGCTGAAAGCCTTTGGGGAATGCCAACCTCTTCGGGAGCTAGCCAACCTGGCACTGATACTTGGTAACAGAGATGACATTGAAGACATGTCAAATAGCAGCTCAGTTGTTCTAACAACCGTGCTCAAGATGATTGACAAGTATGATCTGTATGGTCAAGTGGCCTATCCTAAGCATCACAAGCAATCAGATGTCCCTGACATCTATCGTCTAGCTGCAAAAACAAAGGGAGTGTTCGTCAACCCAGCTCTTGTGGAACCTTTCGGTCTCACTATTATAGAGGCAGCTGCTTATGGTTTACCAGTGGTCGCCACCAGAAACGGAGGACCTGTGGATATTCTGAAGGCACTACACAATGGCCTTCTAATTGACCCACATGACCAGAAGGCCATAGAAGATGCACTACTAAAGCTTGTTGCCGACAAAAACCTCTGGACGGAATGCAGAAAGAATGGCCTAAAGAACATCCACCGCTTTTCGTGGCCAGAACACTGCCGAAACTACCTCTCCCATGTTGAACACTCTCGAAACCGCCACCCTACCACCCGCCTTCAAATCGTACCAGCTCCTGAAGAACCAATGAGTGACTCTCTGAAAGACGTGGATGACCTTTCTTTGCGATTCTCTGTAGACGGAGATTTCAAACATAATAGTGAGCATGATGCAGCAACTAGACAGAGGGAACTCATCGAAGCCATAACCAGAATGACTTCATCCAATAGCACTGCAGCTGCTACTTACTGTCCTGGTAGGAGGCAGAGGCTGTTTGTAATAGCAGTGGATTGTTATGATCAGAATGGAAATGGTACTCAGACGTTTCAGGAAATTATATCGAGTGTCAAGAAAGCAGCAAGCTTAGGATTTGGCCAAGGTCGTGCAGGCTTCGTATTGTTAACTGGTTCCAGCTTACAAGAGACTGTGAAGGCTTTTAAAGGGTGCCAAGTAAGCATAGAAGAATTTGATGCATTGGTCTGCAAAAGTGGGAGTGAAATGTATTACCCTTGGAGAGATTTGGCAGCTGATGCAGATTTTGAAACACACATCGAGTACCGATGGCCTGGAGAGAATGTGAGGTCCATGGTGCCCAGGCTAGCCATTTTAGAAGGCGGGGCTGAGGATGACATCACGGAGTATGGAGGGTCAAGTAGCTCAAGGTGCTACTCTTATAATGTGAAACCAGGAGCCAAGACTCGGCGCGTCGATGACCTGCGCCAAAGGTTGCGGATGCGAGGCTTCCGTTGCAACCTTTTCTACACTCGTGTAGCTTCAAGGTTAAATGTTGTACCACTCTTTGCATCAAGAGTACAAGCACTGAGATATCTTTCGGTCAGGTGGGGAACTGATCTATCCAAAGTGGTTGTGTTTGTGGGGGAAAAAGGAGATACAGATAATGAAGACCTGCTGGCTGGTCTCCACAAGACACTAGTTTTAAGAGGATCAGTAGAGTATGGCAGTGAGAGACTTCTTCATAGTGAAGATGGTTTCAGAAGAGATGATGTAGTCCCACAAGATAGCCCTAACATTGCCCTTGTAGAGAGCTATCAACCCCACGATATCTCAGCCACTCTTGAAGCTCTTGGGATAAAGGGACTTTAG

Protein Analysis

1027

Amino Acids

115.59

Weight (kDa)

7.14

Isoelectric Point (pI)

46.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_trans_4_4 PF13579 196 - 388 7.3e-07 Glycosyl transferase 4-like domain
Glycos_transf_1 PF00534 475 - 648 5e-25 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 479 - 636 2.6e-16 Glycosyl transferases group 1
S6PP PF05116 787 - 957 4.4e-17 Sucrose-6F-phosphate phosphohydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2652
Acc36I ACCTGC 2 cut(s) 2700, 2892
AccB1I GGYRCC 4 cut(s) 336, 974, 2421, 2566
AccI GTMKAC 2 cut(s) 690, 2115
AccII CGCG 2 cut(s) 789, 2682
AclWI GGATC 2 cut(s) 1106, 2929
AcoI YGGCCR 5 cut(s) 627, 853, 931, 1967, 2350
AcsI RAATTY 2 cut(s) 833, 2441
AcuI CTGAAG 6 cut(s) 417, 1128, 1282, 1344, 1847, 2076
AcyI GRCGYC 2 cut(s) 337, 1231
AdeI CACNNNGTG 1 cut(s) 862
AfaI GTAC 7 cut(s) 797, 1150, 2046, 2293, 2534, 2769, 2790
AfiI CCNNNNNNNGG 4 cut(s) 737, 740, 1921, 2676
AflIII ACRYGT 1 cut(s) 1572
AhlI ACTAGT 2 cut(s) 1145, 2908
AjiI CACGTC 1 cut(s) 2086
AjnI CCWGG 7 cut(s) 30, 1285, 1533, 2230, 2542, 2570, 2662
Alw21I GWGCWC 3 cut(s) 625, 880, 1611
Alw26I GTCTC 7 cut(s) 413, 1222, 1458, 1757, 2393, 2900, 2938
AlwI GGATC 2 cut(s) 1106, 2929
AlwNI CAGNNNCTG 2 cut(s) 2054, 2248
ApoI RAATTY 2 cut(s) 833, 2441
Asp700I GAANNNNTTC 2 cut(s) 574, 2194
AspA2I CCTAGG 1 cut(s) 1012
AspLEI GCGC 4 cut(s) 273, 339, 2682, 2697
AspS9I GGNCC 5 cut(s) 379, 544, 782, 1811, 2559
AsuC2I CCSGG 2 cut(s) 1003, 1235
AsuHPI GGTGA 5 cut(s) 428, 607, 672, 716, 1153
AsuNHI GCTAGC 2 cut(s) 1525, 2575
AvaII GGWCC 5 cut(s) 379, 544, 782, 1811, 2559
AvrII CCTAGG 1 cut(s) 1012
BaeGI GKGCMC 1 cut(s) 2571
BalI TGGCCA 3 cut(s) 629, 1969, 2352
BanI GGYRCC 4 cut(s) 336, 974, 2421, 2566
BanII GRGCYC 2 cut(s) 850, 880
BarI GAAGNNNNNNTAC 2 cut(s) 1872, 1904
BauI CACGAG 3 cut(s) 234, 618, 2742
BbsI GAAGAC 3 cut(s) 764, 1575, 2886
Bbv12I GWGCWC 3 cut(s) 625, 880, 1611
BbvCI CCTCAGC 1 cut(s) 2598
BccI CCATC 6 cut(s) 77, 836, 1181, 1325, 2532, 2957
BceAI ACGGC 4 cut(s) 324, 350, 754, 946
BcgI CGANNNNNNTGC 2 cut(s) 269, 303
BciT130I CCWGG 7 cut(s) 32, 1287, 1535, 2232, 2544, 2572, 2664
BclI TGATCA 1 cut(s) 2275
BcnI CCSGG 2 cut(s) 1003, 1235
BcoDI GTCTC 7 cut(s) 413, 1222, 1458, 1757, 2393, 2900, 2938
BcuI ACTAGT 2 cut(s) 1145, 2908
BfmI CTRYAG 5 cut(s) 372, 646, 694, 2112, 2211
BfoI RGCGCY 1 cut(s) 340
BfuAI ACCTGC 2 cut(s) 2700, 2892
BglI GCCNNNNNGGC 1 cut(s) 1535
BglII AGATCT 2 cut(s) 1336, 1364
BlnI CCTAGG 1 cut(s) 1012
BmcAI AGTACT 1 cut(s) 1150
Bme1390I CCNGG 9 cut(s) 32, 1003, 1235, 1287, 1535, 2232, 2544, 2572, 2664
Bme18I GGWCC 5 cut(s) 379, 544, 782, 1811, 2559
BmgBI CACGTC 1 cut(s) 2086
BmgT120I GGNCC 5 cut(s) 379, 544, 782, 1811, 2559
BmiI GGNNCC 9 cut(s) 338, 757, 976, 1078, 1743, 2386, 2423, 2568, 2668
BmrFI CCNGG 9 cut(s) 32, 1003, 1235, 1287, 1535, 2232, 2544, 2572, 2664
BmrI ACTGGG 1 cut(s) 575
BmtI GCTAGC 2 cut(s) 1529, 2579
BmuI ACTGGG 1 cut(s) 575
BpiI GAAGAC 3 cut(s) 764, 1575, 2886
BplI GAGNNNNNCTC 4 cut(s) 217, 249, 2059, 2091
BpmI CTGGAG 3 cut(s) 53, 365, 2565
Bpu10I CCTNAGC 4 cut(s) 1217, 1656, 2341, 2598
BpuEI CTTGAG 4 cut(s) 275, 1184, 1595, 2620
BpuMI CCSGG 2 cut(s) 1003, 1235
BsaBI GATNNNNATC 1 cut(s) 1335
BsaHI GRCGYC 2 cut(s) 337, 1231
BsaI GGTCTC 3 cut(s) 413, 1757, 2900
BsaWI WCCGGW 1 cut(s) 682
Bsc4I CCNNNNNNNGG 4 cut(s) 737, 740, 1921, 2676
Bse1I ACTGG 4 cut(s) 20, 570, 1787, 2386
Bse3DI GCAATG 2 cut(s) 1484, 3006
Bse8I GATNNNNATC 1 cut(s) 1335
BseBI CCWGG 7 cut(s) 32, 1287, 1535, 2232, 2544, 2572, 2664
BseGI GGATG 9 cut(s) 52, 319, 496, 1129, 1950, 2095, 2198, 2608, 2715
BseJI GATNNNNATC 1 cut(s) 1335
BseLI CCNNNNNNNGG 4 cut(s) 737, 740, 1921, 2676
BseMI GCAATG 2 cut(s) 1484, 3006
BseNI ACTGG 4 cut(s) 20, 570, 1787, 2386
BseRI GAGGAG 1 cut(s) 140
BseSI GKGCMC 1 cut(s) 2571
BseYI CCCAGC 3 cut(s) 947, 1114, 1729
BsgI GTGCAG 1 cut(s) 2382
Bsh1236I CGCG 2 cut(s) 789, 2682
BshNI GGYRCC 4 cut(s) 336, 974, 2421, 2566
BsiHKAI GWGCWC 3 cut(s) 625, 880, 1611
BsiSI CCGG 3 cut(s) 683, 1002, 1234
BslFI GGGAC 5 cut(s) 126, 971, 1191, 1664, 2972
BslI CCNNNNNNNGG 4 cut(s) 737, 740, 1921, 2676
BsmAI GTCTC 7 cut(s) 413, 1222, 1458, 1757, 2393, 2900, 2938
BsmBI CGTCTC 1 cut(s) 1222
BsmFI GGGAC 5 cut(s) 126, 971, 1191, 1664, 2972
BsmI GAATGC 3 cut(s) 1006, 1511, 1931
Bso31I GGTCTC 3 cut(s) 413, 1757, 2900
Bsp1286I GDGCHC 5 cut(s) 625, 850, 880, 1611, 2571
Bsp143I GATC 9 cut(s) 655, 1098, 1195, 1336, 1364, 1630, 2275, 2827, 2921
Bsp19I CCATGG 2 cut(s) 990, 2562
Bsp68I TCGCGA 1 cut(s) 789
BspFNI CGCG 2 cut(s) 789, 2682
BspLI GGNNCC 9 cut(s) 338, 757, 976, 1078, 1743, 2386, 2423, 2568, 2668
BspMAI CTGCAG 2 cut(s) 376, 2215
BspMI ACCTGC 2 cut(s) 2700, 2892
BspOI GCTAGC 2 cut(s) 1529, 2579
BspPI GGATC 2 cut(s) 1106, 2929
BspQI GCTCTTC 2 cut(s) 1104, 1161
BspT107I GGYRCC 4 cut(s) 336, 974, 2421, 2566
BspTNI GGTCTC 3 cut(s) 413, 1757, 2900
BsrDI GCAATG 2 cut(s) 1484, 3006
BsrI ACTGG 4 cut(s) 20, 570, 1787, 2386
BssMI GATC 9 cut(s) 655, 1098, 1195, 1336, 1364, 1630, 2275, 2827, 2921
BssNI GRCGYC 2 cut(s) 337, 1231
BssSI CACGAG 3 cut(s) 234, 618, 2742
BssT1I CCWWGG 6 cut(s) 990, 1012, 1249, 2353, 2486, 2562
Bst2BI CACGAG 3 cut(s) 234, 618, 2742
Bst2UI CCWGG 7 cut(s) 32, 1287, 1535, 2232, 2544, 2572, 2664
Bst4CI ACNGT 7 cut(s) 26, 704, 1182, 1312, 1606, 2228, 2404
Bst6I CTCTTC 7 cut(s) 565, 882, 1088, 1104, 1161, 1521, 2968
BstACI GRCGYC 2 cut(s) 337, 1231
BstAPI GCANNNNNTGC 1 cut(s) 2507
BstC8I GCNNGC 8 cut(s) 269, 322, 731, 1527, 2338, 2365, 2577, 2889
BstDSI CCRYGG 4 cut(s) 547, 928, 990, 2562
BstEII GGTNACC 1 cut(s) 26
BstF5I GGATG 9 cut(s) 52, 319, 496, 1129, 1950, 2095, 2198, 2608, 2715
BstFNI CGCG 2 cut(s) 789, 2682
BstH2I RGCGCY 1 cut(s) 340
BstHHI GCGC 4 cut(s) 273, 339, 2682, 2697
BstKTI GATC 9 cut(s) 658, 1101, 1198, 1339, 1367, 1633, 2278, 2830, 2924
BstMAI GTCTC 7 cut(s) 413, 1222, 1458, 1757, 2393, 2900, 2938
BstMBI GATC 9 cut(s) 655, 1098, 1195, 1336, 1364, 1630, 2275, 2827, 2921
BstNI CCWGG 7 cut(s) 32, 1287, 1535, 2232, 2544, 2572, 2664
BstNSI RCATGY 2 cut(s) 1264, 1576
BstPI GGTNACC 1 cut(s) 26
BstSCI CCNGG 9 cut(s) 30, 1001, 1233, 1285, 1533, 2230, 2542, 2570, 2662
BstSFI CTRYAG 5 cut(s) 372, 646, 694, 2112, 2211
BstSLI GKGCMC 1 cut(s) 2571
BstUI CGCG 2 cut(s) 789, 2682
BstV2I GAAGAC 3 cut(s) 764, 1575, 2886
BstX2I RGATCY 2 cut(s) 1336, 1364
BstYI RGATCY 2 cut(s) 1336, 1364
BtgI CCRYGG 4 cut(s) 547, 928, 990, 2562
BtgZI GCGATG 1 cut(s) 96
BtrI CACGTC 1 cut(s) 2086
BtsCI GGATG 9 cut(s) 52, 319, 496, 1129, 1950, 2095, 2198, 2608, 2715
BtsI GCAGTG 5 cut(s) 1178, 1975, 2208, 2268, 2944
BtsIMutI CAGTG 9 cut(s) 1178, 1317, 1538, 1794, 1975, 2208, 2268, 2795, 2944
BtuMI TCGCGA 1 cut(s) 789
BveI ACCTGC 2 cut(s) 2700, 2892
Cac8I GCNNGC 8 cut(s) 269, 322, 731, 1527, 2338, 2365, 2577, 2889
CaiI CAGNNNCTG 2 cut(s) 2054, 2248
CfoI GCGC 4 cut(s) 273, 339, 2682, 2697
Cfr13I GGNCC 5 cut(s) 379, 544, 782, 1811, 2559
CseI GACGC 3 cut(s) 377, 1239, 2671
Csp6I GTAC 7 cut(s) 796, 1149, 2045, 2292, 2533, 2768, 2789
CspCI CAANNNNNGTGG 2 cut(s) 1254, 1289
CviQI GTAC 7 cut(s) 796, 1149, 2045, 2292, 2533, 2768, 2789
DinI GGCGCC 1 cut(s) 338
DpnI GATC 9 cut(s) 657, 1100, 1197, 1338, 1366, 1632, 2277, 2829, 2923
DpnII GATC 9 cut(s) 655, 1098, 1195, 1336, 1364, 1630, 2275, 2827, 2921
DraI TTTAAA 1 cut(s) 2416
DraIII CACNNNGTG 1 cut(s) 862
EaeI YGGCCR 5 cut(s) 627, 853, 931, 1967, 2350
Eam1104I CTCTTC 7 cut(s) 565, 882, 1088, 1104, 1161, 1521, 2968
EarI CTCTTC 7 cut(s) 565, 882, 1088, 1104, 1161, 1521, 2968
Ecl136II GAGCTC 1 cut(s) 878
Eco130I CCWWGG 6 cut(s) 990, 1012, 1249, 2353, 2486, 2562
Eco24I GRGCYC 2 cut(s) 850, 880
Eco31I GGTCTC 3 cut(s) 413, 1757, 2900
Eco32I GATATC 2 cut(s) 2804, 3040
Eco47I GGWCC 5 cut(s) 379, 544, 782, 1811, 2559
Eco53kI GAGCTC 1 cut(s) 878
Eco57I CTGAAG 6 cut(s) 417, 1128, 1282, 1344, 1847, 2076
Eco91I GGTNACC 1 cut(s) 26
EcoICRI GAGCTC 1 cut(s) 878
EcoO109I RGGNCCY 2 cut(s) 379, 1811
EcoO65I GGTNACC 1 cut(s) 26
EcoRII CCWGG 7 cut(s) 30, 1285, 1533, 2230, 2542, 2570, 2662
EcoRV GATATC 2 cut(s) 2804, 3040
EcoT14I CCWWGG 6 cut(s) 990, 1012, 1249, 2353, 2486, 2562
EcoT22I ATGCAT 2 cut(s) 537, 2452
EcoT38I GRGCYC 2 cut(s) 850, 880
EgeI GGCGCC 1 cut(s) 338
EheI GGCGCC 1 cut(s) 338
ErhI CCWWGG 6 cut(s) 990, 1012, 1249, 2353, 2486, 2562
Esp3I CGTCTC 1 cut(s) 1222
FaqI GGGAC 5 cut(s) 126, 971, 1191, 1664, 2972
FauI CCCGC 3 cut(s) 738, 2039, 2585
FbaI TGATCA 1 cut(s) 2275
FblI GTMKAC 2 cut(s) 690, 2115
FokI GGATG 9 cut(s) 59, 326, 503, 1136, 1937, 2102, 2185, 2615, 2722
FriOI GRGCYC 2 cut(s) 850, 880
GlaI GCGC 4 cut(s) 272, 338, 2681, 2696
GsaI CCCAGC 3 cut(s) 951, 1118, 1733
GsuI CTGGAG 3 cut(s) 53, 365, 2565
HaeII RGCGCY 1 cut(s) 340
HapII CCGG 3 cut(s) 683, 1002, 1234
HgaI GACGC 3 cut(s) 377, 1239, 2671
HhaI GCGC 4 cut(s) 273, 339, 2682, 2697
Hin1I GRCGYC 2 cut(s) 337, 1231
Hin6I GCGC 4 cut(s) 271, 337, 2680, 2695
HinP1I GCGC 4 cut(s) 271, 337, 2680, 2695
HincII GTYRAC 4 cut(s) 172, 663, 1726, 2379
HindII GTYRAC 4 cut(s) 172, 663, 1726, 2379
HindIII AAGCTT 2 cut(s) 1895, 2338
HpaI GTTAAC 3 cut(s) 172, 663, 2379
HpaII CCGG 3 cut(s) 683, 1002, 1234
HphI GGTGA 5 cut(s) 428, 607, 672, 716, 1153
Hpy99I CGWCG 1 cut(s) 2687
HpyCH4III ACNGT 7 cut(s) 26, 704, 1182, 1312, 1606, 2228, 2404
HpyCH4IV ACGT 2 cut(s) 2085, 2300
HpySE526I ACGT 2 cut(s) 2085, 2300
Hsp92I GRCGYC 2 cut(s) 337, 1231
HspAI GCGC 4 cut(s) 271, 337, 2680, 2695
KasI GGCGCC 1 cut(s) 336
Ksp22I TGATCA 1 cut(s) 2275
KspAI GTTAAC 3 cut(s) 172, 663, 2379
Kzo9I GATC 9 cut(s) 655, 1098, 1195, 1336, 1364, 1630, 2275, 2827, 2921
LguI GCTCTTC 2 cut(s) 1104, 1161
LmnI GCTCC 5 cut(s) 78, 755, 1522, 2056, 2666
MaeII ACGT 2 cut(s) 2085, 2300
MaeIII GTNAC 8 cut(s) 26, 62, 810, 1141, 1306, 1477, 1550, 2069
MalI GATC 9 cut(s) 657, 1100, 1197, 1338, 1366, 1632, 2277, 2829, 2923
MboI GATC 9 cut(s) 655, 1098, 1195, 1336, 1364, 1630, 2275, 2827, 2921
MflI RGATCY 2 cut(s) 1336, 1364
MhlI GDGCHC 5 cut(s) 625, 850, 880, 1611, 2571
MlsI TGGCCA 3 cut(s) 629, 1969, 2352
MluCI AATT 4 cut(s) 833, 1851, 2310, 2441
MluNI TGGCCA 3 cut(s) 629, 1969, 2352
Mly113I GGCGCC 1 cut(s) 337
MlyI GAGTC 5 cut(s) 194, 651, 818, 2066, 2668
MmeI TCCRAC 1 cut(s) 1185
Mox20I TGGCCA 3 cut(s) 629, 1969, 2352
Mph1103I ATGCAT 2 cut(s) 537, 2452
MroXI GAANNNNTTC 2 cut(s) 574, 2194
MscI TGGCCA 3 cut(s) 629, 1969, 2352
MseI TTAA 9 cut(s) 20, 171, 210, 524, 662, 2378, 2415, 2759, 2915
MslI CAYNNNNRTG 1 cut(s) 2136
Msp20I TGGCCA 3 cut(s) 629, 1969, 2352
MspA1I CMGCKG 4 cut(s) 750, 1772, 2216, 2504
MspI CCGG 3 cut(s) 683, 1002, 1234
MspR9I CCNGG 9 cut(s) 32, 1003, 1235, 1287, 1535, 2232, 2544, 2572, 2664
Mva1269I GAATGC 3 cut(s) 1006, 1511, 1931
MvaI CCWGG 7 cut(s) 32, 1287, 1535, 2232, 2544, 2572, 2664
MvnI CGCG 2 cut(s) 789, 2682
NarI GGCGCC 1 cut(s) 337
NciI CCSGG 2 cut(s) 1003, 1235
NcoI CCATGG 2 cut(s) 990, 2562
NdeII GATC 9 cut(s) 655, 1098, 1195, 1336, 1364, 1630, 2275, 2827, 2921
NheI GCTAGC 2 cut(s) 1525, 2575
NlaIV GGNNCC 9 cut(s) 338, 757, 976, 1078, 1743, 2386, 2423, 2568, 2668
NmeAIII GCCGAG 2 cut(s) 831, 2656
NmuCI GTSAC 3 cut(s) 810, 1141, 2069
NruI TCGCGA 1 cut(s) 789
NsiI ATGCAT 2 cut(s) 537, 2452
NspI RCATGY 2 cut(s) 1264, 1576
PciI ACATGT 1 cut(s) 1572
PciSI GCTCTTC 2 cut(s) 1104, 1161
PctI GAATGC 3 cut(s) 1006, 1511, 1931
PdmI GAANNNNTTC 2 cut(s) 574, 2194
PfeI GAWTC 5 cut(s) 40, 459, 575, 1381, 2106
PleI GAGTC 5 cut(s) 193, 650, 817, 2066, 2668
PluTI GGCGCC 1 cut(s) 340
PpsI GAGTC 5 cut(s) 193, 650, 817, 2066, 2668
PpuMI RGGWCCY 2 cut(s) 379, 1811
PscI ACATGT 1 cut(s) 1572
PsiI TTATAA 1 cut(s) 2652
Psp124BI GAGCTC 1 cut(s) 880
Psp5II RGGWCCY 2 cut(s) 379, 1811
Psp6I CCWGG 7 cut(s) 30, 1285, 1533, 2230, 2542, 2570, 2662
PspEI GGTNACC 1 cut(s) 26
PspFI CCCAGC 3 cut(s) 947, 1114, 1729
PspGI CCWGG 7 cut(s) 30, 1285, 1533, 2230, 2542, 2570, 2662
PspN4I GGNNCC 9 cut(s) 338, 757, 976, 1078, 1743, 2386, 2423, 2568, 2668
PspPI GGNCC 5 cut(s) 379, 544, 782, 1811, 2559
PspPPI RGGWCCY 2 cut(s) 379, 1811
PstI CTGCAG 2 cut(s) 376, 2215
PstNI CAGNNNCTG 2 cut(s) 2054, 2248
PsuI RGATCY 2 cut(s) 1336, 1364
PvuII CAGCTG 4 cut(s) 750, 1772, 2216, 2504
RruI TCGCGA 1 cut(s) 789
RsaI GTAC 7 cut(s) 797, 1150, 2046, 2293, 2534, 2769, 2790
RsaNI GTAC 7 cut(s) 796, 1149, 2045, 2292, 2533, 2768, 2789
RseI CAYNNNNRTG 1 cut(s) 2136
SacI GAGCTC 1 cut(s) 880
SapI GCTCTTC 2 cut(s) 1104, 1161
SaqAI TTAA 9 cut(s) 20, 171, 210, 524, 662, 2378, 2415, 2759, 2915
Sau3AI GATC 9 cut(s) 655, 1098, 1195, 1336, 1364, 1630, 2275, 2827, 2921
Sau96I GGNCC 5 cut(s) 379, 544, 782, 1811, 2559
ScaI AGTACT 1 cut(s) 1150
SchI GAGTC 5 cut(s) 194, 651, 818, 2066, 2668
ScrFI CCNGG 9 cut(s) 32, 1003, 1235, 1287, 1535, 2232, 2544, 2572, 2664
SduI GDGCHC 5 cut(s) 625, 850, 880, 1611, 2571
SfcI CTRYAG 5 cut(s) 372, 646, 694, 2112, 2211
SfoI GGCGCC 1 cut(s) 338
SinI GGWCC 5 cut(s) 379, 544, 782, 1811, 2559
SmiMI CAYNNNNRTG 1 cut(s) 2136
SmlI CTYRAG 4 cut(s) 254, 1199, 1610, 2635
SmoI CTYRAG 4 cut(s) 254, 1199, 1610, 2635
SpeI ACTAGT 2 cut(s) 1145, 2908
Sse9I AATT 4 cut(s) 833, 1851, 2310, 2441
SspDI GGCGCC 1 cut(s) 336
SstI GAGCTC 1 cut(s) 880
StyD4I CCNGG 9 cut(s) 30, 1001, 1233, 1285, 1533, 2230, 2542, 2570, 2662
StyI CCWWGG 6 cut(s) 990, 1012, 1249, 2353, 2486, 2562
TaaI ACNGT 7 cut(s) 26, 704, 1182, 1312, 1606, 2228, 2404
TaiI ACGT 2 cut(s) 2088, 2303
TaqI TCGA 7 cut(s) 1058, 1101, 2011, 2175, 2318, 2529, 2685
TaqII GACCGA 2 cut(s) 1739, 2800
TasI AATT 4 cut(s) 833, 1851, 2310, 2441
TatI WGTACW 3 cut(s) 795, 1148, 2788
TauI GCSGC 3 cut(s) 363, 744, 747
TfiI GAWTC 5 cut(s) 40, 459, 575, 1381, 2106
Tru1I TTAA 9 cut(s) 20, 171, 210, 524, 662, 2378, 2415, 2759, 2915
Tru9I TTAA 9 cut(s) 20, 171, 210, 524, 662, 2378, 2415, 2759, 2915
TscAI CASTG 9 cut(s) 1178, 1317, 1545, 1794, 1982, 2215, 2268, 2802, 2944
TseFI GTSAC 3 cut(s) 810, 1141, 2069
Tsp45I GTSAC 3 cut(s) 810, 1141, 2069
TspDTI ATGAA 4 cut(s) 1106, 2187, 2892, 2942
TspGWI ACGGA 7 cut(s) 536, 758, 1821, 1937, 2133, 2627, 2711
TspRI CASTG 9 cut(s) 1178, 1317, 1545, 1794, 1982, 2215, 2268, 2802, 2944
VpaK11BI GGWCC 5 cut(s) 379, 544, 782, 1811, 2559
XapI RAATTY 2 cut(s) 833, 2441
XceI RCATGY 2 cut(s) 1264, 1576
XmaJI CCTAGG 1 cut(s) 1012
XmiI GTMKAC 2 cut(s) 690, 2115
XmnI GAANNNNTTC 2 cut(s) 574, 2194
ZrmI AGTACT 1 cut(s) 1150
Zsp2I ATGCAT 2 cut(s) 537, 2452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.