MD10G1002500.v1.1

Belongs to the glycosyltransferase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0006699
Physical Location & Seq
Reverse (-)
557866 .. 560708
2843 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1002500.v1.1.491

Sequence Viewer

Length: 1491 bp
ATGGCGGGAAACGACTGGCTAAATGGTTATTTAGAAGCAATTTTGGATGCAGGAAATAACACAAGGAAGAGGAACGACGGGAGGCAGAAAATTGCAAAGTTTGAAGAGCAAGTAAAGGCTGAGAAGTTGTTCAGTCCGACCAAGTACTTTGTGGAAGAAGTAGTCAATAGCTTTGATGAGTCTGAACTCCACAGGACATGGGTTAAGGTAATAGCTACAAGGAATACCCGCGAAAGCAGCAATAGGCTTGAGAATATGTGCTGGCGCATTTGGCACCTCGCCCGTAAAAAGAAGCAGATAGCATGGGATGACGCTCAAAGGCTAGTCAAACGACGCCTGGAGCGGGAGCAAGGGCGCAATGACGCGGAGGATGACCTTTCCGAGCTGTCTGAAGGCGAGAAGGAAAAAGGGGACATGAGTTCTGCAGAACCAAGTGTCAAGGATATTTTAAGAACTAAATCCGACATGCCTGTATGGTCCGATGATGTTAACAAGTCTAGGCATCTTTACATTGTCCTAATCAGCGTGCATGGGTTGGTGCGTGGAGAAAACATGGAACTCGGAAGAGATTCCGATACAGGCGGTCAGGTGAAGTATGTGGTGGAACTTGCTCGTGCTCTCGCAAATACAAAGGGTGTCTACCGAGTTGATCTGCTAACGAGACAAATTACTTCACCGGAGGTAGACTCCAGCTACGGTGAACCAAATGAGATGCTCATATGTCCGCCTGATGGAAGTGGCAGCTGTGGAGCCTACATTGTCCGCCTCCCCTGTGGACCCCGCGATAAGTACATACCCAAAGAGTCACTTTGGCCGCACATACCTGAATTCGTCGATGGCGCTCTCGGCCACATTGTGAACATGGCAAGAGCTCTTGGTGAAGAAGTCAATGGGGGGAAACCCACATGGCCTTATGTGATTCATGGTCACTATGCTGATGCAGGGGATGTGGCTGCTCACTTGTCTGGGGCTTTGAACGTGCCAATGGTGCTAACTGGGCATTCGTTAGGCCGAAACAAGTTTGAACAATTGCTCAAACAAGGGAGGTTGACAAAGGAGGATATCAACGCAACCTACAAGATCATGAGGAGGATCGAAGGTGAGGAGCTGGGATTGGATTCTGCTGAAATGGTGGTCACCAGCACTAGGCAAGAGATCGAAGAGCAATGGGGACTCTACGATGGCTTTGACCTCAAGTTGGAGAGGAAGCTTAGGGTTAGGAGACGTCGTGGAGTTAGTTGCCTTGGGCGATACATGCCAAGAATGGTGGTTATACCGCCAGGCATGGACTTCAGCTATGTTACTGCCCATGATTCCGAAGGAGATGGAGATCTCAAATCCTTGATTGGCTCTGATAGAGGTCAAAGCAAAAGGCATCTGCCTCCGATATGGTCTGAGGTTGCAAATTCTTCCACCTATTTTCTCCTTTCACATATTCCCATCCCCTTCAATTGGTTATACAGGTATATGCATTTCGTCCACCGAAAATGA

Protein Analysis

497

Amino Acids

56.48

Weight (kDa)

6.96

Isoelectric Point (pI)

48.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_trans_4_4 PF13579 194 - 387 4.1e-08 Glycosyl transferase 4-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1228
AccB1I GGYRCC 1 cut(s) 273
AccBSI CCGCTC 1 cut(s) 343
AccI GTMKAC 2 cut(s) 639, 684
AccII CGCG 3 cut(s) 231, 365, 783
AclWI GGATC 1 cut(s) 1100
AcoI YGGCCR 2 cut(s) 812, 847
AcsI RAATTY 2 cut(s) 827, 1405
AcuI CTGAAG 2 cut(s) 411, 1276
AcyI GRCGYC 2 cut(s) 334, 1225
AdeI CACNNNGTG 1 cut(s) 856
AfaI GTAC 2 cut(s) 146, 791
AfiI CCNNNNNNNGG 5 cut(s) 343, 731, 1146, 1198, 1452
AgsI TTSAA 4 cut(s) 104, 976, 1025, 1450
AjnI CCWGG 2 cut(s) 336, 1279
AluBI AGCT 9 cut(s) 171, 215, 385, 693, 744, 872, 1108, 1210, 1296
AluI AGCT 9 cut(s) 171, 215, 385, 693, 744, 872, 1108, 1210, 1296
Alw21I GWGCWC 2 cut(s) 619, 874
Alw26I GTCTC 2 cut(s) 655, 1216
AlwI GGATC 1 cut(s) 1100
AoxI GGCC 4 cut(s) 812, 847, 908, 1009
ApeKI GCWGC 3 cut(s) 237, 741, 953
ApoI RAATTY 2 cut(s) 827, 1405
Asp700I GAANNNNTTC 2 cut(s) 128, 568
AspLEI GCGC 3 cut(s) 267, 357, 842
AspS9I GGNCC 2 cut(s) 477, 776
AsuHPI GGTGA 6 cut(s) 601, 666, 710, 890, 1112, 1129
AvaII GGWCC 2 cut(s) 477, 776
BanI GGYRCC 1 cut(s) 273
BanII GRGCYC 1 cut(s) 874
BauI CACGAG 1 cut(s) 612
Bbv12I GWGCWC 2 cut(s) 619, 874
BbvI GCAGC 3 cut(s) 249, 753, 940
BccI CCATC 5 cut(s) 725, 830, 1175, 1319, 1448
BciT130I CCWGG 2 cut(s) 338, 1281
BcoDI GTCTC 2 cut(s) 655, 1216
BfaI CTAG 3 cut(s) 323, 498, 1146
BfmI CTRYAG 1 cut(s) 423
BfoI RGCGCY 1 cut(s) 843
BglII AGATCT 1 cut(s) 1330
BisI GCNGC 4 cut(s) 238, 742, 815, 954
BlsI GCNGC 4 cut(s) 239, 743, 816, 955
BmcAI AGTACT 1 cut(s) 146
Bme1390I CCNGG 2 cut(s) 338, 1281
Bme18I GGWCC 2 cut(s) 477, 776
BmgT120I GGNCC 2 cut(s) 477, 776
BmiI GGNNCC 3 cut(s) 275, 751, 778
BmrFI CCNGG 2 cut(s) 338, 1281
BmrI ACTGGG 1 cut(s) 1005
BmsI GCATC 5 cut(s) 37, 511, 702, 928, 1384
BmuI ACTGGG 1 cut(s) 1005
BplI GAGNNNNNCTC 2 cut(s) 671, 703
BpmI CTGGAG 2 cut(s) 359, 673
Bpu10I CCTNAGC 1 cut(s) 1211
BpuEI CTTGAG 2 cut(s) 269, 1178
BsaBI GATNNNNATC 1 cut(s) 1329
BsaHI GRCGYC 2 cut(s) 334, 1225
BsaJI CCNNGG 1 cut(s) 1243
BsaWI WCCGGW 1 cut(s) 676
BsaXI ACNNNNNCTCC 2 cut(s) 1213, 1243
Bsc4I CCNNNNNNNGG 5 cut(s) 343, 731, 1146, 1198, 1452
Bse1I ACTGG 2 cut(s) 20, 1000
Bse3DI GCAATG 2 cut(s) 364, 1172
Bse8I GATNNNNATC 1 cut(s) 1329
BseBI CCWGG 2 cut(s) 338, 1281
BseDI CCNNGG 1 cut(s) 1243
BseGI GGATG 5 cut(s) 52, 313, 376, 952, 1440
BseJI GATNNNNATC 1 cut(s) 1329
BseLI CCNNNNNNNGG 5 cut(s) 343, 731, 1146, 1198, 1452
BseMI GCAATG 2 cut(s) 364, 1172
BseMII CTCAG 2 cut(s) 111, 1386
BseNI ACTGG 2 cut(s) 20, 1000
BseRI GAGGAG 2 cut(s) 1102, 1118
BseXI GCAGC 3 cut(s) 249, 753, 940
BseYI CCCAGC 1 cut(s) 1108
Bsh1236I CGCG 3 cut(s) 231, 365, 783
BshFI GGCC 4 cut(s) 814, 849, 910, 1011
BshNI GGYRCC 1 cut(s) 273
BsiHKAI GWGCWC 2 cut(s) 619, 874
BsiSI CCGG 1 cut(s) 677
BslFI GGGAC 2 cut(s) 425, 1185
BslI CCNNNNNNNGG 5 cut(s) 343, 731, 1146, 1198, 1452
BsmAI GTCTC 2 cut(s) 655, 1216
BsmBI CGTCTC 1 cut(s) 1216
BsmFI GGGAC 2 cut(s) 425, 1185
BsmI GAATGC 1 cut(s) 1000
BsnI GGCC 4 cut(s) 814, 849, 910, 1011
Bsp1286I GDGCHC 2 cut(s) 619, 874
Bsp143I GATC 5 cut(s) 649, 1080, 1092, 1155, 1330
BspANI GGCC 4 cut(s) 814, 849, 910, 1011
BspCNI CTCAG 2 cut(s) 112, 1387
BspFNI CGCG 3 cut(s) 231, 365, 783
BspHI TCATGA 1 cut(s) 1083
BspLI GGNNCC 3 cut(s) 275, 751, 778
BspMAI CTGCAG 1 cut(s) 427
BspPI GGATC 1 cut(s) 1100
BspQI GCTCTTC 2 cut(s) 99, 1155
BspT107I GGYRCC 1 cut(s) 273
BsrBI CCGCTC 1 cut(s) 343
BsrDI GCAATG 2 cut(s) 364, 1172
BsrI ACTGG 2 cut(s) 20, 1000
BssECI CCNNGG 1 cut(s) 1243
BssMI GATC 5 cut(s) 649, 1080, 1092, 1155, 1330
BssNI GRCGYC 2 cut(s) 334, 1225
BssSI CACGAG 1 cut(s) 612
BssT1I CCWWGG 1 cut(s) 1243
Bst2BI CACGAG 1 cut(s) 612
Bst2UI CCWGG 2 cut(s) 338, 1281
Bst4CI ACNGT 1 cut(s) 698
Bst6I CTCTTC 4 cut(s) 62, 99, 559, 1155
BstACI GRCGYC 2 cut(s) 334, 1225
BstC8I GCNNGC 2 cut(s) 263, 527
BstDEI CTNAG 3 cut(s) 120, 1211, 1395
BstEII GGTNACC 1 cut(s) 1135
BstF5I GGATG 5 cut(s) 52, 313, 376, 952, 1440
BstFNI CGCG 3 cut(s) 231, 365, 783
BstH2I RGCGCY 1 cut(s) 843
BstHHI GCGC 3 cut(s) 267, 357, 842
BstKTI GATC 5 cut(s) 652, 1083, 1095, 1158, 1333
BstMAI GTCTC 2 cut(s) 655, 1216
BstMBI GATC 5 cut(s) 649, 1080, 1092, 1155, 1330
BstMWI GCNNNNNNNGC 6 cut(s) 237, 271, 846, 988, 997, 1255
BstNI CCWGG 2 cut(s) 338, 1281
BstNSI RCATGY 2 cut(s) 469, 1258
BstPI GGTNACC 1 cut(s) 1135
BstSCI CCNGG 2 cut(s) 336, 1279
BstSFI CTRYAG 1 cut(s) 423
BstUI CGCG 3 cut(s) 231, 365, 783
BstV1I GCAGC 3 cut(s) 249, 753, 940
BstX2I RGATCY 1 cut(s) 1330
BstYI RGATCY 1 cut(s) 1330
BsuRI GGCC 4 cut(s) 814, 849, 910, 1011
BtsCI GGATG 5 cut(s) 52, 313, 376, 952, 1440
Cac8I GCNNGC 2 cut(s) 263, 527
CciI TCATGA 1 cut(s) 1083
CfoI GCGC 3 cut(s) 267, 357, 842
Cfr13I GGNCC 2 cut(s) 477, 776
CseI GACGC 3 cut(s) 320, 342, 371
Csp6I GTAC 2 cut(s) 145, 790
CspCI CAANNNNNGTGG 2 cut(s) 1248, 1283
CviQI GTAC 2 cut(s) 145, 790
DdeI CTNAG 3 cut(s) 120, 1211, 1395
DpnI GATC 5 cut(s) 651, 1082, 1094, 1157, 1332
DpnII GATC 5 cut(s) 649, 1080, 1092, 1155, 1330
DraIII CACNNNGTG 1 cut(s) 856
EaeI YGGCCR 2 cut(s) 812, 847
Eam1104I CTCTTC 4 cut(s) 62, 99, 559, 1155
EarI CTCTTC 4 cut(s) 62, 99, 559, 1155
EciI GGCGGA 2 cut(s) 714, 752
Ecl136II GAGCTC 1 cut(s) 872
Eco130I CCWWGG 1 cut(s) 1243
Eco24I GRGCYC 1 cut(s) 874
Eco32I GATATC 1 cut(s) 1063
Eco47I GGWCC 2 cut(s) 477, 776
Eco53kI GAGCTC 1 cut(s) 872
Eco57I CTGAAG 2 cut(s) 411, 1276
Eco91I GGTNACC 1 cut(s) 1135
EcoICRI GAGCTC 1 cut(s) 872
EcoO65I GGTNACC 1 cut(s) 1135
EcoRI GAATTC 1 cut(s) 827
EcoRII CCWGG 2 cut(s) 336, 1279
EcoRV GATATC 1 cut(s) 1063
EcoT14I CCWWGG 1 cut(s) 1243
EcoT22I ATGCAT 1 cut(s) 1473
EcoT38I GRGCYC 1 cut(s) 874
ErhI CCWWGG 1 cut(s) 1243
Esp3I CGTCTC 1 cut(s) 1216
FalI AAGNNNNNCTT 2 cut(s) 792, 824
FaqI GGGAC 2 cut(s) 425, 1185
FauI CCCGC 3 cut(s) 236, 336, 788
FauNDI CATATG 1 cut(s) 719
FblI GTMKAC 2 cut(s) 639, 684
Fnu4HI GCNGC 4 cut(s) 238, 742, 815, 954
FokI GGATG 5 cut(s) 59, 320, 383, 959, 1427
FriOI GRGCYC 1 cut(s) 874
Fsp4HI GCNGC 4 cut(s) 238, 742, 815, 954
FspBI CTAG 3 cut(s) 323, 498, 1146
GlaI GCGC 3 cut(s) 266, 356, 841
GluI GCNGC 4 cut(s) 238, 742, 815, 954
GsaI CCCAGC 1 cut(s) 1112
GsuI CTGGAG 2 cut(s) 359, 673
HaeII RGCGCY 1 cut(s) 843
HaeIII GGCC 4 cut(s) 814, 849, 910, 1011
HapII CCGG 1 cut(s) 677
HgaI GACGC 3 cut(s) 320, 342, 371
HhaI GCGC 3 cut(s) 267, 357, 842
Hin1I GRCGYC 2 cut(s) 334, 1225
Hin6I GCGC 3 cut(s) 265, 355, 840
HinP1I GCGC 3 cut(s) 265, 355, 840
HincII GTYRAC 2 cut(s) 490, 1050
HindII GTYRAC 2 cut(s) 490, 1050
HindIII AAGCTT 1 cut(s) 1208
HinfI GANTC 8 cut(s) 179, 569, 686, 803, 919, 1118, 1173, 1313
HpaI GTTAAC 1 cut(s) 490
HpaII CCGG 1 cut(s) 677
HphI GGTGA 6 cut(s) 601, 666, 710, 890, 1112, 1129
Hpy166II GTNNAC 8 cut(s) 490, 640, 685, 701, 776, 859, 1050, 1480
Hpy188III TCNNGA 1 cut(s) 1084
Hpy8I GTNNAC 8 cut(s) 490, 640, 685, 701, 776, 859, 1050, 1480
Hpy99I CGWCG 4 cut(s) 80, 336, 836, 1230
HpyAV CCTTC 5 cut(s) 386, 394, 1091, 1313, 1456
HpyCH4III ACNGT 1 cut(s) 698
HpyCH4IV ACGT 2 cut(s) 978, 1225
HpyCH4V TGCA 7 cut(s) 50, 95, 425, 529, 941, 1403, 1471
HpyF10VI GCNNNNNNNGC 6 cut(s) 237, 271, 846, 988, 997, 1255
HpyF3I CTNAG 3 cut(s) 120, 1211, 1395
HpySE526I ACGT 2 cut(s) 978, 1225
Hsp92I GRCGYC 2 cut(s) 334, 1225
HspAI GCGC 3 cut(s) 265, 355, 840
KspAI GTTAAC 1 cut(s) 490
Kzo9I GATC 5 cut(s) 649, 1080, 1092, 1155, 1330
LguI GCTCTTC 2 cut(s) 99, 1155
LmnI GCTCC 4 cut(s) 340, 346, 749, 1105
Lsp1109I GCAGC 3 cut(s) 249, 753, 940
LweI GCATC 5 cut(s) 37, 511, 702, 928, 1384
MaeI CTAG 3 cut(s) 323, 498, 1146
MaeII ACGT 2 cut(s) 978, 1225
MaeIII GTNAC 4 cut(s) 804, 926, 1135, 1300
MalI GATC 5 cut(s) 651, 1082, 1094, 1157, 1332
MbiI CCGCTC 1 cut(s) 343
MboI GATC 5 cut(s) 649, 1080, 1092, 1155, 1330
MboII GAAGA 7 cut(s) 79, 116, 167, 576, 893, 1172, 1401
MfeI CAATTG 2 cut(s) 1028, 1450
MflI RGATCY 1 cut(s) 1330
MhlI GDGCHC 2 cut(s) 619, 874
MluCI AATT 7 cut(s) 39, 90, 666, 827, 1028, 1405, 1450
MlyI GAGTC 4 cut(s) 188, 680, 812, 1167
MmeI TCCRAC 3 cut(s) 161, 486, 1179
Mph1103I ATGCAT 1 cut(s) 1473
MroXI GAANNNNTTC 2 cut(s) 128, 568
MseI TTAA 3 cut(s) 204, 449, 489
MspA1I CMGCKG 1 cut(s) 744
MspI CCGG 1 cut(s) 677
MspR9I CCNGG 2 cut(s) 338, 1281
MunI CAATTG 2 cut(s) 1028, 1450
Mva1269I GAATGC 1 cut(s) 1000
MvaI CCWGG 2 cut(s) 338, 1281
MvnI CGCG 3 cut(s) 231, 365, 783
MwoI GCNNNNNNNGC 6 cut(s) 237, 271, 846, 988, 997, 1255
NdeI CATATG 1 cut(s) 719
NdeII GATC 5 cut(s) 649, 1080, 1092, 1155, 1330
NlaIV GGNNCC 3 cut(s) 275, 751, 778
NmeAIII GCCGAG 1 cut(s) 825
NmuCI GTSAC 3 cut(s) 804, 926, 1135
NsiI ATGCAT 1 cut(s) 1473
NspI RCATGY 2 cut(s) 469, 1258
PagI TCATGA 1 cut(s) 1083
PciSI GCTCTTC 2 cut(s) 99, 1155
PctI GAATGC 1 cut(s) 1000
PdmI GAANNNNTTC 2 cut(s) 128, 568
PfeI GAWTC 4 cut(s) 569, 919, 1118, 1313
PkrI GCNGC 4 cut(s) 239, 743, 816, 955
PleI GAGTC 4 cut(s) 187, 680, 811, 1167
PpsI GAGTC 4 cut(s) 187, 680, 811, 1167
Psp124BI GAGCTC 1 cut(s) 874
Psp6I CCWGG 2 cut(s) 336, 1279
PspEI GGTNACC 1 cut(s) 1135
PspFI CCCAGC 1 cut(s) 1108
PspGI CCWGG 2 cut(s) 336, 1279
PspN4I GGNNCC 3 cut(s) 275, 751, 778
PspPI GGNCC 2 cut(s) 477, 776
PstI CTGCAG 1 cut(s) 427
PsuI RGATCY 1 cut(s) 1330
PvuII CAGCTG 1 cut(s) 744
RsaI GTAC 2 cut(s) 146, 791
RsaNI GTAC 2 cut(s) 145, 790
SacI GAGCTC 1 cut(s) 874
SapI GCTCTTC 2 cut(s) 99, 1155
SaqAI TTAA 3 cut(s) 204, 449, 489
SatI GCNGC 4 cut(s) 238, 742, 815, 954
Sau3AI GATC 5 cut(s) 649, 1080, 1092, 1155, 1330
Sau96I GGNCC 2 cut(s) 477, 776
ScaI AGTACT 1 cut(s) 146
SchI GAGTC 4 cut(s) 188, 680, 812, 1167
ScrFI CCNGG 2 cut(s) 338, 1281
SduI GDGCHC 2 cut(s) 619, 874
SfaNI GCATC 5 cut(s) 37, 511, 702, 928, 1384
SfcI CTRYAG 1 cut(s) 423
SinI GGWCC 2 cut(s) 477, 776
SmlI CTYRAG 2 cut(s) 248, 1193
SmoI CTYRAG 2 cut(s) 248, 1193
Sse9I AATT 7 cut(s) 39, 90, 666, 827, 1028, 1405, 1450
SspMI CTAG 3 cut(s) 323, 498, 1146
SstI GAGCTC 1 cut(s) 874
StyD4I CCNGG 2 cut(s) 336, 1279
StyI CCWWGG 1 cut(s) 1243
TaaI ACNGT 1 cut(s) 698
TaiI ACGT 2 cut(s) 981, 1228
TaqI TCGA 3 cut(s) 834, 1095, 1158
TasI AATT 7 cut(s) 39, 90, 666, 827, 1028, 1405, 1450
TatI WGTACW 2 cut(s) 144, 789
TauI GCSGC 1 cut(s) 817
TfiI GAWTC 4 cut(s) 569, 919, 1118, 1313
Tru1I TTAA 3 cut(s) 204, 449, 489
Tru9I TTAA 3 cut(s) 204, 449, 489
TseFI GTSAC 3 cut(s) 804, 926, 1135
TseI GCWGC 3 cut(s) 237, 741, 953
Tsp45I GTSAC 3 cut(s) 804, 926, 1135
TspDTI ATGAA 1 cut(s) 911
VpaK11BI GGWCC 2 cut(s) 477, 776
XapI RAATTY 2 cut(s) 827, 1405
XceI RCATGY 2 cut(s) 469, 1258
XcmI CCANNNNNNNNNTGG 1 cut(s) 148
XmiI GTMKAC 2 cut(s) 639, 684
XmnI GAANNNNTTC 2 cut(s) 128, 568
XspI CTAG 3 cut(s) 323, 498, 1146
ZraI GACGTC 1 cut(s) 1226
ZrmI AGTACT 1 cut(s) 146
Zsp2I ATGCAT 1 cut(s) 1473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.