Rh6BG006100

Belongs to the glycosyltransferase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0006699
Physical Location & Seq
Forward (+)
942883 .. 948324
5442 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG006100.1

Sequence Viewer

Length: 3075 bp
ATGGCGGGAAACGACTGGTTAAACGGTTATCTCGAGGCGATTCTGGATGCAGGAATTAACACAAGGAAAACGAACGATGGGAAGCAGAAGATTGCCAAGTTTGAAGAGCAAGTCAAGGAGGAGAAGTTGTTTAGTCCCACAAAGTACTTCGTGGAAGAAGTAGTTAGCAGCTTTGATGAGTCTGACCTCCACAGGACATGGGTTAAGGTAGTAGCTACAAGGAATACCCGTGAACGCAGCAATAGGCTTGAGAATATGTGCTGGCGCATTTGGCATCTCGCTCGTAAGAAGAAACAGATAGCATGGGATGATGCTCGCAGACTTGCAAGACGACGTCTGGAGAGGGAGCAAGGCCGCCATGATGCTGCGGAGGACCTTTCAGAGCTGTCTGAAGGGGAGAAGGAAAAAGGTGACACCAATTTTACAGAACCGCCTGTCAAGCAAATTGCAAGAATCAACTCAGAAATGCGATTGTGGTCTGAGGATGACAACAAGACTAGGCATCTTTACATTGTCTTAATCAGTATGCATGGGTTGGTTCGTGGAGAAAACATGGAACTTGGAAGAGATTCTGATACAGGTGGTCAGGTGAAGTATGTAGTGGAACTTGCTCGTGCTCTGGCCAATACAAAAGGAGTCTATAGAGTTGATCTGTTAACAAGGCAAATCACTTCACCGGAGGTAGACTCCAGCTACGGTGAACCTAATGAGATGCTCATTTGCCCACCTGATGGCAGCGGGAGCTGTGGAGCCTACATTGTCCGCCTTCCATGTGGACCCCGCGACAAGTACATACCGAAAGAGTCACTATGGCCTCACATACCTGAATTTATTGATGGGGCTCTCGGCCACATTGTGAACATGGCAAGAGCTCTAGGCGATGAGGTCAATGGTGGGAAACCAACATGGCCTTATGTCATCCACGGCCACTATGCTGATGCAGGGGAGGTGGCTGCTCAGTTGTCTGGTGCCTTGAATGTGCCAATGGTGCTGACGGGGCATTCCCTAGGAAGAAACAAGTTTGAACAACTGCTCAAACAGGGGAGATTGTCGAGGGAGGACATCAATGCAACCTACAAGATTATAAAGAGGATCGAGGCTGAGGAGCTGGCTTTGGATGCTGCTGAAATGGTGGTGACTAGTACTAGGCAAGAGATTGAAGAGCAGTGGGGACTGTATGATGGGTTCGATCTCAAGTTGGAGAGGAAGCTCAGGGTTAGGAGACGCCGGGGAGTTAGTTGCCTTGGACGATACATGCCAAGAATGGTGGTTATACCGCCAGGCATGGACTTCAGCTATGTTACAGTGCAAGATACTGAAGGAGATGGAGATCTCAAATCCCTGATTGGTTCTGACAGAGCTCAAAGCAAAAGGAATCTCCCTCCAATATGGTCTGAGGTAATGCGATTTTTCACTAATCCTCACAAGCCCACTATACTTGCATTGTCTCGTCCTGACCCAAAGAAAAATGTTACTACATTGCTGAAGGCCTTTGGGGAATGCAAACCGCTTCGGGAGCTAGCCAACCTGGCACTGATACTTGGTAACAGAGATGACATTGAAGAAATGCCAAATAGCAGCTCGGTTGTTCTAACAACTGTGCTCAAGCTGATCGACAGGTATGATCTGTATGGTCAAGTGGCCTATCCTAAACATCACAAGCAATCAGATGTCCCTGACATATATCGTCTAGCTGCAAAAACAAAGGGAGTGTTTATCAACCCAGCTCTTGTGGAACCTTTCGGTCTCACTATTATAGAGGCAGCTGCTTATGGTTTACCAGTGGTTGCCACCAGAAACGGAGGACCTGTGGACATTCTGAAGGCACTAAACAATGGCCTCCTAGTTGACCCACATGACCAGAAGGCCATAGAAGAAGCACTGCTAAAGCTTGTCGCAGATAAAAACCTCTGGTTGGAATGCAGAAAGAATGGCCTAAAGAACATCCACCGCTTTTCGTGGCCAGAACACTGCCGAAACTACCTCTCCCATGTTGAACACTCCCGAAACCGCCACCCTACCACCCGCATTCAGATCGTACCAATTCCTGAAGAACCAATGAGCGACTCTCTAAAAGACGTGGAGGACCTTTCTTTGAGATTCTCTGTAGACGGAGATTTCAAGCATAATAGTGAGCTTGATGCAGCAACTAGACAGAGGGAACTCATTGAAGCCATAACCAAAATGGCTTCATCCAATAGCAATGCTGGTGCTACTTACTGTCCTGGTAGGAGGCAGAGGTTGTTTGTAATAGCCGTGGACTGTTATGACCAGAATGGAGATGGCACTCAGACGTTTCAGGAAATTATAACGAGTGTCAAGAAAGCAGCAAGCTTAGGATTTGGCCAGGGTCGTGTAGGCTTCGTATTGTTAACTGGTTCCAGCTTACAAGAAACCGTGAAGGCATTTAAAGGGTGCCAAGTAAGCATAGAAGAATTTGATGCGGTGGTCTGCAAAAGTGGCAGTGAAATGTATTACCCATGGAGAGATTTGGCAGCTGATGCAGATTTTGAAAGTCATATCGAGTACAGATGGCCTGGAGAGAATGTGAGGTCCATGGTGCCAAGGCTGGCCATTTTAGAAGGTGGGGCTGAGGATGACATCACGGAGTATGGAGGATCAAGTAGCTCAAGGTGCTACTCTTATAATGTGAAACCAGGAGCCAAGACTCGGCGAATCGATGACCTGCGCCAAAGGCTGCGGATGCGAGGCTTCCGATGCAACCTTTTCTACACTCGTGCAGCATCAAGGTTAAATGTTGTACCACTCTTTGCATCGAGAGTACAAGCACTGAGATATCTTTCGGTCAGGTGGGGAACTGATTTATCCAAAGTGGTTATGTTTGTGGGGGAAAAAGGAGATACAGATAATGAAGACCTGCTGGCTGGTCTCCATAAGACACTAGTTTTAAGAGGATCCGTTGAGTATGGCAGTGAGAAGCTTCTTCATAGTGAAGATGGTTTCAGAAGAGATGATGTAGTCCCACAAGATAGCCCCAACATTGCCCTTGTAGAGAGCTATCAACCCCACGATATCTCAGCCACTCTTGAAGCTCTTGGAATAAAGGGACTTTAG

Protein Analysis

1024

Amino Acids

115.31

Weight (kDa)

6.7

Isoelectric Point (pI)

47.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_trans_4_4 PF13579 194 - 387 1.9e-07 Glycosyl transferase 4-like domain
GT-B_Sucrose_synth PF00862 263 - 432 3.9e-06 Sucrose synthase first GT-B domain
Glycos_transf_1 PF00534 473 - 646 2.4e-24 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 477 - 634 1e-16 Glycosyl transferases group 1
S6PP PF05116 784 - 955 2.8e-17 Sucrose-6F-phosphate phosphohydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1085, 2309, 2646
AatII GACGTC 1 cut(s) 337
Acc36I ACCTGC 2 cut(s) 2694, 2886
AccB1I GGYRCC 3 cut(s) 968, 2415, 2560
AccB7I CCANNNNNTGG 1 cut(s) 731
AccI GTMKAC 2 cut(s) 684, 2109
AccII CGCG 1 cut(s) 783
AclWI GGATC 4 cut(s) 1100, 2626, 2910, 2923
AcoI YGGCCR 6 cut(s) 621, 847, 925, 1961, 2344, 2571
AcsI RAATTY 2 cut(s) 827, 2435
AcuI CTGAAG 6 cut(s) 411, 1276, 1338, 1505, 1841, 2070
AcyI GRCGYC 2 cut(s) 334, 1225
AdeI CACNNNGTG 1 cut(s) 856
AfaI GTAC 7 cut(s) 146, 791, 1144, 2040, 2528, 2763, 2784
AfiI CCNNNNNNNGG 3 cut(s) 731, 1915, 2670
AhlI ACTAGT 2 cut(s) 1139, 2902
AjiI CACGTC 1 cut(s) 2080
AjnI CCWGG 6 cut(s) 1279, 1527, 2224, 2346, 2536, 2656
Alw21I GWGCWC 4 cut(s) 619, 874, 1363, 1605
Alw26I GTCTC 4 cut(s) 1216, 1452, 1751, 2894
AlwI GGATC 4 cut(s) 1100, 2626, 2910, 2923
Ama87I CYCGRG 1 cut(s) 32
ApoI RAATTY 2 cut(s) 827, 2435
Asp700I GAANNNNTTC 1 cut(s) 568
AspA2I CCTAGG 1 cut(s) 1006
AspLEI GCGC 2 cut(s) 267, 2691
AspS9I GGNCC 5 cut(s) 373, 776, 1805, 2086, 2553
AsuC2I CCSGG 1 cut(s) 1229
AsuHPI GGTGA 5 cut(s) 422, 601, 666, 710, 1147
AsuNHI GCTAGC 1 cut(s) 1519
AvaI CYCGRG 1 cut(s) 32
AvaII GGWCC 5 cut(s) 373, 776, 1805, 2086, 2553
AvrII CCTAGG 1 cut(s) 1006
BalI TGGCCA 4 cut(s) 623, 1963, 2346, 2573
BamHI GGATCC 1 cut(s) 2915
BanI GGYRCC 3 cut(s) 968, 2415, 2560
BanII GRGCYC 3 cut(s) 844, 874, 1363
BauI CACGAG 2 cut(s) 612, 2736
BbsI GAAGAC 1 cut(s) 2880
Bbv12I GWGCWC 4 cut(s) 619, 874, 1363, 1605
BbvCI CCTCAGC 2 cut(s) 1101, 2592
BccI CCATC 8 cut(s) 71, 725, 830, 1175, 1319, 2276, 2526, 2951
BceAI ACGGC 2 cut(s) 940, 2240
BcgI CGANNNNNNTGC 4 cut(s) 263, 297, 2017, 2051
BciT130I CCWGG 6 cut(s) 1281, 1529, 2226, 2348, 2538, 2658
BcnI CCSGG 1 cut(s) 1229
BcoDI GTCTC 4 cut(s) 1216, 1452, 1751, 2894
BcuI ACTAGT 2 cut(s) 1139, 2902
BfmI CTRYAG 2 cut(s) 640, 2106
BfuAI ACCTGC 2 cut(s) 2694, 2886
BglI GCCNNNNNGGC 1 cut(s) 1529
BglII AGATCT 1 cut(s) 1330
BlnI CCTAGG 1 cut(s) 1006
BmcAI AGTACT 2 cut(s) 146, 1144
Bme1390I CCNGG 7 cut(s) 1229, 1281, 1529, 2226, 2348, 2538, 2658
Bme18I GGWCC 5 cut(s) 373, 776, 1805, 2086, 2553
BmeT110I CYCGRG 1 cut(s) 32
BmgBI CACGTC 1 cut(s) 2080
BmgT120I GGNCC 5 cut(s) 373, 776, 1805, 2086, 2553
BmiI GGNNCC 9 cut(s) 751, 778, 970, 1737, 2380, 2417, 2562, 2662, 2917
BmrFI CCNGG 7 cut(s) 1229, 1281, 1529, 2226, 2348, 2538, 2658
BmtI GCTAGC 1 cut(s) 1523
BpiI GAAGAC 1 cut(s) 2880
BplI GAGNNNNNCTC 4 cut(s) 671, 703, 2053, 2085
BpmI CTGGAG 3 cut(s) 359, 673, 2559
Bpu10I CCTNAGC 4 cut(s) 1101, 1211, 2335, 2592
BpuEI CTTGAG 4 cut(s) 269, 1178, 1589, 2614
BpuMI CCSGG 1 cut(s) 1229
Bsa29I ATCGAT 1 cut(s) 2679
BsaBI GATNNNNATC 1 cut(s) 1329
BsaHI GRCGYC 2 cut(s) 334, 1225
BsaI GGTCTC 2 cut(s) 1751, 2894
BsaJI CCNNGG 9 cut(s) 922, 1006, 1228, 1243, 2256, 2347, 2480, 2556, 2564
BsaWI WCCGGW 1 cut(s) 676
Bsc4I CCNNNNNNNGG 3 cut(s) 731, 1915, 2670
Bse1I ACTGG 3 cut(s) 20, 1781, 2380
Bse3DI GCAATG 3 cut(s) 1478, 2209, 3000
Bse8I GATNNNNATC 1 cut(s) 1329
BseBI CCWGG 6 cut(s) 1281, 1529, 2226, 2348, 2538, 2658
BseCI ATCGAT 1 cut(s) 2679
BseDI CCNNGG 9 cut(s) 922, 1006, 1228, 1243, 2256, 2347, 2480, 2556, 2564
BseGI GGATG 9 cut(s) 52, 313, 490, 918, 1123, 1944, 2192, 2602, 2709
BseJI GATNNNNATC 1 cut(s) 1329
BseLI CCNNNNNNNGG 3 cut(s) 731, 1915, 2670
BseMI GCAATG 3 cut(s) 1478, 2209, 3000
BseNI ACTGG 3 cut(s) 20, 1781, 2380
BseRI GAGGAG 2 cut(s) 134, 1118
BseYI CCCAGC 1 cut(s) 1723
BsgI GTGCAG 1 cut(s) 2760
Bsh1236I CGCG 1 cut(s) 783
BshNI GGYRCC 3 cut(s) 968, 2415, 2560
BshVI ATCGAT 1 cut(s) 2679
BsiHKAI GWGCWC 4 cut(s) 619, 874, 1363, 1605
BsiHKCI CYCGRG 1 cut(s) 32
BsiSI CCGG 2 cut(s) 677, 1228
BslFI GGGAC 4 cut(s) 120, 1185, 1658, 2966
BslI CCNNNNNNNGG 3 cut(s) 731, 1915, 2670
BsmAI GTCTC 4 cut(s) 1216, 1452, 1751, 2894
BsmBI CGTCTC 1 cut(s) 1216
BsmFI GGGAC 4 cut(s) 120, 1185, 1658, 2966
BsmI GAATGC 4 cut(s) 1000, 1505, 1925, 2028
Bso31I GGTCTC 2 cut(s) 1751, 2894
BsoBI CYCGRG 1 cut(s) 32
Bsp1286I GDGCHC 5 cut(s) 619, 844, 874, 1363, 1605
Bsp143I GATC 9 cut(s) 649, 1092, 1189, 1330, 1611, 1624, 2034, 2618, 2915
Bsp19I CCATGG 2 cut(s) 2480, 2556
BspDI ATCGAT 1 cut(s) 2679
BspFNI CGCG 1 cut(s) 783
BspLI GGNNCC 9 cut(s) 751, 778, 970, 1737, 2380, 2417, 2562, 2662, 2917
BspMI ACCTGC 2 cut(s) 2694, 2886
BspOI GCTAGC 1 cut(s) 1523
BspPI GGATC 4 cut(s) 1100, 2626, 2910, 2923
BspQI GCTCTTC 2 cut(s) 99, 1155
BspT107I GGYRCC 3 cut(s) 968, 2415, 2560
BspTNI GGTCTC 2 cut(s) 1751, 2894
BsrDI GCAATG 3 cut(s) 1478, 2209, 3000
BsrI ACTGG 3 cut(s) 20, 1781, 2380
BssECI CCNNGG 9 cut(s) 922, 1006, 1228, 1243, 2256, 2347, 2480, 2556, 2564
BssMI GATC 9 cut(s) 649, 1092, 1189, 1330, 1611, 1624, 2034, 2618, 2915
BssNI GRCGYC 2 cut(s) 334, 1225
BssSI CACGAG 2 cut(s) 612, 2736
BssT1I CCWWGG 5 cut(s) 1006, 1243, 2480, 2556, 2564
Bst2BI CACGAG 2 cut(s) 612, 2736
Bst2UI CCWGG 6 cut(s) 1281, 1529, 2226, 2348, 2538, 2658
Bst4CI ACNGT 8 cut(s) 26, 698, 1176, 1306, 1600, 2222, 2264, 2398
Bst6I CTCTTC 4 cut(s) 99, 559, 1155, 2962
BstACI GRCGYC 2 cut(s) 334, 1225
BstAPI GCANNNNNTGC 1 cut(s) 2501
BstC8I GCNNGC 7 cut(s) 263, 316, 1110, 1521, 2332, 2571, 2883
BstDSI CCRYGG 4 cut(s) 922, 2256, 2480, 2556
BstF5I GGATG 9 cut(s) 52, 313, 490, 918, 1123, 1944, 2192, 2602, 2709
BstFNI CGCG 1 cut(s) 783
BstHHI GCGC 2 cut(s) 267, 2691
BstKTI GATC 9 cut(s) 652, 1095, 1192, 1333, 1614, 1627, 2037, 2621, 2918
BstMAI GTCTC 4 cut(s) 1216, 1452, 1751, 2894
BstMBI GATC 9 cut(s) 649, 1092, 1189, 1330, 1611, 1624, 2034, 2618, 2915
BstNI CCWGG 6 cut(s) 1281, 1529, 2226, 2348, 2538, 2658
BstNSI RCATGY 1 cut(s) 1258
BstSCI CCNGG 7 cut(s) 1227, 1279, 1527, 2224, 2346, 2536, 2656
BstSFI CTRYAG 2 cut(s) 640, 2106
BstUI CGCG 1 cut(s) 783
BstV2I GAAGAC 1 cut(s) 2880
BstX2I RGATCY 2 cut(s) 1330, 2915
BstYI RGATCY 2 cut(s) 1330, 2915
Bsu15I ATCGAT 1 cut(s) 2679
BsuTUI ATCGAT 1 cut(s) 2679
BtgI CCRYGG 4 cut(s) 922, 2256, 2480, 2556
BtgZI GCGATG 1 cut(s) 894
BtrI CACGTC 1 cut(s) 2080
BtsCI GGATG 9 cut(s) 52, 313, 490, 918, 1123, 1944, 2192, 2602, 2709
BtsI GCAGTG 5 cut(s) 1172, 1880, 1969, 2470, 2938
BtsIMutI CAGTG 9 cut(s) 1172, 1311, 1532, 1788, 1880, 1969, 2470, 2789, 2938
BveI ACCTGC 2 cut(s) 2694, 2886
Cac8I GCNNGC 7 cut(s) 263, 316, 1110, 1521, 2332, 2571, 2883
CfoI GCGC 2 cut(s) 267, 2691
Cfr13I GGNCC 5 cut(s) 373, 776, 1805, 2086, 2553
ClaI ATCGAT 1 cut(s) 2679
CseI GACGC 1 cut(s) 1233
Csp6I GTAC 7 cut(s) 145, 790, 1143, 2039, 2527, 2762, 2783
CspCI CAANNNNNGTGG 4 cut(s) 1248, 1283, 1420, 1455
CviQI GTAC 7 cut(s) 145, 790, 1143, 2039, 2527, 2762, 2783
DpnI GATC 9 cut(s) 651, 1094, 1191, 1332, 1613, 1626, 2036, 2620, 2917
DpnII GATC 9 cut(s) 649, 1092, 1189, 1330, 1611, 1624, 2034, 2618, 2915
DraI TTTAAA 1 cut(s) 2410
DraIII CACNNNGTG 1 cut(s) 856
EaeI YGGCCR 6 cut(s) 621, 847, 925, 1961, 2344, 2571
Eam1104I CTCTTC 4 cut(s) 99, 559, 1155, 2962
EarI CTCTTC 4 cut(s) 99, 559, 1155, 2962
EciI GGCGGA 1 cut(s) 752
Ecl136II GAGCTC 2 cut(s) 872, 1361
Eco130I CCWWGG 5 cut(s) 1006, 1243, 2480, 2556, 2564
Eco147I AGGCCT 1 cut(s) 1490
Eco24I GRGCYC 3 cut(s) 844, 874, 1363
Eco31I GGTCTC 2 cut(s) 1751, 2894
Eco32I GATATC 2 cut(s) 2798, 3034
Eco47I GGWCC 5 cut(s) 373, 776, 1805, 2086, 2553
Eco53kI GAGCTC 2 cut(s) 872, 1361
Eco57I CTGAAG 6 cut(s) 411, 1276, 1338, 1505, 1841, 2070
Eco88I CYCGRG 1 cut(s) 32
EcoICRI GAGCTC 2 cut(s) 872, 1361
EcoO109I RGGNCCY 3 cut(s) 373, 1805, 2086
EcoRII CCWGG 6 cut(s) 1279, 1527, 2224, 2346, 2536, 2656
EcoRV GATATC 2 cut(s) 2798, 3034
EcoT14I CCWWGG 5 cut(s) 1006, 1243, 2480, 2556, 2564
EcoT22I ATGCAT 1 cut(s) 531
EcoT38I GRGCYC 3 cut(s) 844, 874, 1363
ErhI CCWWGG 5 cut(s) 1006, 1243, 2480, 2556, 2564
Esp3I CGTCTC 1 cut(s) 1216
FaqI GGGAC 4 cut(s) 120, 1185, 1658, 2966
FauI CCCGC 3 cut(s) 731, 788, 2033
FblI GTMKAC 2 cut(s) 684, 2109
FokI GGATG 9 cut(s) 59, 320, 497, 905, 1130, 1931, 2179, 2609, 2716
FriOI GRGCYC 3 cut(s) 844, 874, 1363
GlaI GCGC 2 cut(s) 266, 2690
GsaI CCCAGC 1 cut(s) 1727
GsuI CTGGAG 3 cut(s) 359, 673, 2559
HapII CCGG 2 cut(s) 677, 1228
HgaI GACGC 1 cut(s) 1233
HhaI GCGC 2 cut(s) 267, 2691
Hin1I GRCGYC 2 cut(s) 334, 1225
Hin6I GCGC 2 cut(s) 265, 2689
HinP1I GCGC 2 cut(s) 265, 2689
HincII GTYRAC 3 cut(s) 657, 1849, 2373
HindII GTYRAC 3 cut(s) 657, 1849, 2373
HindIII AAGCTT 3 cut(s) 1889, 2332, 2939
HpaI GTTAAC 2 cut(s) 657, 2373
HpaII CCGG 2 cut(s) 677, 1228
HphI GGTGA 5 cut(s) 422, 601, 666, 710, 1147
Hpy99I CGWCG 1 cut(s) 336
HpyAV CCTTC 9 cut(s) 386, 394, 776, 1313, 1480, 1816, 1858, 2395, 2576
HpyCH4III ACNGT 8 cut(s) 26, 698, 1176, 1306, 1600, 2222, 2264, 2398
HpyCH4IV ACGT 3 cut(s) 334, 2079, 2294
HpySE526I ACGT 3 cut(s) 334, 2079, 2294
Hsp92I GRCGYC 2 cut(s) 334, 1225
HspAI GCGC 2 cut(s) 265, 2689
KspAI GTTAAC 2 cut(s) 657, 2373
Kzo9I GATC 9 cut(s) 649, 1092, 1189, 1330, 1611, 1624, 2034, 2618, 2915
LguI GCTCTTC 2 cut(s) 99, 1155
LmnI GCTCC 6 cut(s) 346, 741, 749, 1105, 1516, 2660
MaeII ACGT 3 cut(s) 334, 2079, 2294
MaeIII GTNAC 6 cut(s) 410, 804, 1135, 1300, 1471, 1544
MalI GATC 9 cut(s) 651, 1094, 1191, 1332, 1613, 1626, 2036, 2620, 2917
MboI GATC 9 cut(s) 649, 1092, 1189, 1330, 1611, 1624, 2034, 2618, 2915
MflI RGATCY 2 cut(s) 1330, 2915
MhlI GDGCHC 5 cut(s) 619, 844, 874, 1363, 1605
MlsI TGGCCA 4 cut(s) 623, 1963, 2346, 2573
MluCI AATT 7 cut(s) 54, 418, 444, 827, 2043, 2304, 2435
MluNI TGGCCA 4 cut(s) 623, 1963, 2346, 2573
MlyI GAGTC 6 cut(s) 188, 645, 680, 812, 2060, 2662
MmeI TCCRAC 2 cut(s) 1179, 1896
Mox20I TGGCCA 4 cut(s) 623, 1963, 2346, 2573
Mph1103I ATGCAT 1 cut(s) 531
MroXI GAANNNNTTC 1 cut(s) 568
MscI TGGCCA 4 cut(s) 623, 1963, 2346, 2573
MseI TTAA 9 cut(s) 20, 57, 204, 518, 656, 2372, 2409, 2753, 2909
MslI CAYNNNNRTG 1 cut(s) 2130
Msp20I TGGCCA 4 cut(s) 623, 1963, 2346, 2573
MspA1I CMGCKG 3 cut(s) 738, 1766, 2498
MspI CCGG 2 cut(s) 677, 1228
MspR9I CCNGG 7 cut(s) 1229, 1281, 1529, 2226, 2348, 2538, 2658
Mva1269I GAATGC 4 cut(s) 1000, 1505, 1925, 2028
MvaI CCWGG 6 cut(s) 1281, 1529, 2226, 2348, 2538, 2658
MvnI CGCG 1 cut(s) 783
NciI CCSGG 1 cut(s) 1229
NcoI CCATGG 2 cut(s) 2480, 2556
NdeII GATC 9 cut(s) 649, 1092, 1189, 1330, 1611, 1624, 2034, 2618, 2915
NheI GCTAGC 1 cut(s) 1519
NlaIV GGNNCC 9 cut(s) 751, 778, 970, 1737, 2380, 2417, 2562, 2662, 2917
NmeAIII GCCGAG 2 cut(s) 825, 2650
NmuCI GTSAC 3 cut(s) 410, 804, 1135
NsiI ATGCAT 1 cut(s) 531
NspI RCATGY 1 cut(s) 1258
PaeR7I CTCGAG 1 cut(s) 32
PceI AGGCCT 1 cut(s) 1490
PciSI GCTCTTC 2 cut(s) 99, 1155
PcsI WCGNNNNNNNCGW 1 cut(s) 30
PctI GAATGC 4 cut(s) 1000, 1505, 1925, 2028
PdmI GAANNNNTTC 1 cut(s) 568
PfeI GAWTC 6 cut(s) 40, 453, 569, 1375, 2100, 2676
PflFI GACNNNGTC 1 cut(s) 333
PflMI CCANNNNNTGG 1 cut(s) 731
PleI GAGTC 6 cut(s) 187, 644, 680, 811, 2060, 2662
PpsI GAGTC 6 cut(s) 187, 644, 680, 811, 2060, 2662
PpuMI RGGWCCY 3 cut(s) 373, 1805, 2086
PsiI TTATAA 3 cut(s) 1085, 2309, 2646
Psp124BI GAGCTC 2 cut(s) 874, 1363
Psp5II RGGWCCY 3 cut(s) 373, 1805, 2086
Psp6I CCWGG 6 cut(s) 1279, 1527, 2224, 2346, 2536, 2656
PspFI CCCAGC 1 cut(s) 1723
PspGI CCWGG 6 cut(s) 1279, 1527, 2224, 2346, 2536, 2656
PspN4I GGNNCC 9 cut(s) 751, 778, 970, 1737, 2380, 2417, 2562, 2662, 2917
PspPI GGNCC 5 cut(s) 373, 776, 1805, 2086, 2553
PspPPI RGGWCCY 3 cut(s) 373, 1805, 2086
PsrI GAACNNNNNNTAC 2 cut(s) 1169, 1201
PsuI RGATCY 2 cut(s) 1330, 2915
PsyI GACNNNGTC 1 cut(s) 333
PvuII CAGCTG 2 cut(s) 1766, 2498
RsaI GTAC 7 cut(s) 146, 791, 1144, 2040, 2528, 2763, 2784
RsaNI GTAC 7 cut(s) 145, 790, 1143, 2039, 2527, 2762, 2783
RseI CAYNNNNRTG 1 cut(s) 2130
SacI GAGCTC 2 cut(s) 874, 1363
SapI GCTCTTC 2 cut(s) 99, 1155
SaqAI TTAA 9 cut(s) 20, 57, 204, 518, 656, 2372, 2409, 2753, 2909
Sau3AI GATC 9 cut(s) 649, 1092, 1189, 1330, 1611, 1624, 2034, 2618, 2915
Sau96I GGNCC 5 cut(s) 373, 776, 1805, 2086, 2553
ScaI AGTACT 2 cut(s) 146, 1144
SchI GAGTC 6 cut(s) 188, 645, 680, 812, 2060, 2662
ScrFI CCNGG 7 cut(s) 1229, 1281, 1529, 2226, 2348, 2538, 2658
SduI GDGCHC 5 cut(s) 619, 844, 874, 1363, 1605
SfcI CTRYAG 2 cut(s) 640, 2106
Sfr274I CTCGAG 1 cut(s) 32
SinI GGWCC 5 cut(s) 373, 776, 1805, 2086, 2553
SlaI CTCGAG 1 cut(s) 32
SmiMI CAYNNNNRTG 1 cut(s) 2130
SmlI CTYRAG 5 cut(s) 32, 248, 1193, 1604, 2629
SmoI CTYRAG 5 cut(s) 32, 248, 1193, 1604, 2629
SpeI ACTAGT 2 cut(s) 1139, 2902
Sse9I AATT 7 cut(s) 54, 418, 444, 827, 2043, 2304, 2435
SseBI AGGCCT 1 cut(s) 1490
SstI GAGCTC 2 cut(s) 874, 1363
StuI AGGCCT 1 cut(s) 1490
StyD4I CCNGG 7 cut(s) 1227, 1279, 1527, 2224, 2346, 2536, 2656
StyI CCWWGG 5 cut(s) 1006, 1243, 2480, 2556, 2564
TaaI ACNGT 8 cut(s) 26, 698, 1176, 1306, 1600, 2222, 2264, 2398
TaiI ACGT 3 cut(s) 337, 2082, 2297
TaqI TCGA 8 cut(s) 33, 1052, 1095, 1188, 1614, 2523, 2679, 2777
TaqII GACCGA 2 cut(s) 1733, 2794
TasI AATT 7 cut(s) 54, 418, 444, 827, 2043, 2304, 2435
TatI WGTACW 5 cut(s) 144, 789, 1142, 2526, 2782
TauI GCSGC 1 cut(s) 357
TfiI GAWTC 6 cut(s) 40, 453, 569, 1375, 2100, 2676
Tru1I TTAA 9 cut(s) 20, 57, 204, 518, 656, 2372, 2409, 2753, 2909
Tru9I TTAA 9 cut(s) 20, 57, 204, 518, 656, 2372, 2409, 2753, 2909
TscAI CASTG 9 cut(s) 1172, 1311, 1539, 1788, 1887, 1976, 2470, 2796, 2938
TseFI GTSAC 3 cut(s) 410, 804, 1135
Tsp45I GTSAC 3 cut(s) 410, 804, 1135
TspDTI ATGAA 3 cut(s) 2181, 2886, 2936
TspGWI ACGGA 4 cut(s) 1815, 2127, 2621, 2908
TspRI CASTG 9 cut(s) 1172, 1311, 1539, 1788, 1887, 1976, 2470, 2796, 2938
Tth111I GACNNNGTC 1 cut(s) 333
Van91I CCANNNNNTGG 1 cut(s) 731
VpaK11BI GGWCC 5 cut(s) 373, 776, 1805, 2086, 2553
XapI RAATTY 2 cut(s) 827, 2435
XceI RCATGY 1 cut(s) 1258
XcmI CCANNNNNNNNNTGG 2 cut(s) 2182, 2279
XhoI CTCGAG 1 cut(s) 32
XmaJI CCTAGG 1 cut(s) 1006
XmiI GTMKAC 2 cut(s) 684, 2109
XmnI GAANNNNTTC 1 cut(s) 568
ZraI GACGTC 1 cut(s) 335
ZrmI AGTACT 2 cut(s) 146, 1144
Zsp2I ATGCAT 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.