MD05G1006400.v1.1

Belongs to the glycosyltransferase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006699
Physical Location & Seq
Reverse (-)
1541525 .. 1547329
5805 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1006400.v1.1.491

Sequence Viewer

Length: 3075 bp
ATGGCCGGAAACGACTGGTTAAACGGTTATTTGGAAGCAATTTTGGATGCAGGAAATAACACGAGGAAGAGGGACGACGGGAGGCAGAAAATTTCAAAGTTTGAAGAGCAAGTAAAGGCCGAGAAGTTGTTCAATCCGACCAAGTACTTTGTAGAAGAAGTTGTCAATAGCTTTGATGAGTCTGAACTCCACAGGACATGGATTAAGGTAATAGCTACAAGGAATAGCCGAGAACACAGCAATAGGCTTGAGAATACGTGCTGGCGCATTTGGCATCTCGCCCGTAAAAAGAAACAGATGGCATGGGATGACGCTCAAAGGCTTGTCAAACGACGTCTGGACAGGGAGCAAGGGCGCCGTGACGCGGAGGATGACCTTTCCGAGCTGTCTGAAGGCGAGAAGGAAAAAGGGGACGTGAGTTGCGCAGAACCAACAGTGAAGGATATTTTAAGAAGTAAATCCGACATGCCTGTGTGGTCCGATGATGTTAACAAGTCTAGGCATCTTTACGTTGTCCTGATCAGTATGCATGGGTTGGTGCGTGGAGAAAACATGGAACTCGGAAGAGATTCCGATACAGGCGGTCAGGTCAAGTATGTGATTGAACTTGCTCGTGCTCTGGCTAATACAAAGGGAGTCTACCGAGTTGATCTGCTAACGAGACAAATTACTTCACCGGAGGTAGACTCCAGCTACGGTGAACCCAATGAGATGCTCATATGTCCACCTGATGGAAGTGGCAGCTGTGGAGCCTACATTGTCCGCCTCCCCTGTGGACCCCACGATAAGTATATACCCAAAGAGTCACTTTGGCCTCACATACCTGAATTCGTTGATGGGGCTCTCGGCCACATTGTGAACATGGCAAGAGCTCTTGGTGAAGAAGTCAATGGGGGAAAACCCACATGGCCTTATGTGATCCATGGCCACTATGCCGATGCTGGGGAGGTGGCTGCTCACTTGTCCGGGGCTTTGAACGTGCCAATGGTGCTAACTGGGCATTCGTTAGGCCGAAACAAGTTTGAACAATTGCTCAAACAAGGGAGGTTGACAAAGGAGGACATCAACGCAACCTACAAAATCATGAGGAGGATCGAGGGTGAGGAGCTGGGGTTGGATTCTGCTGAAACGGTGGTCACCAGCACTAGGCAAGAAATCGAAGAGCAATGGGGACTCTACGATGGCTTTGACCTCAAGTTGGAGAGGAAACTTAGGGTTAGGAGACGTCGTGGAGTCAGTTGCCTTGGACGATACATGCCAAGAATGGTGGTTATACCGCCAGGCATGGACTTCAGCTGTGTTACTGCCCATGATTCCGAAGGAGATGGAGATCTCAAATCCTTGATTGGCTCTGATAGAGGTCAAAGCAAAAGGCATCTGCCTCCAATCTGGTCTGAGGTAATGCGATTTTTTACCAACCCTCACAAGCCCACTATACTTGCATTGTCTCGTCCTGACCCCAAGAAAAATGTTACTACCTTGCTCAAGGCCTTTGGGGAATGCCGAGCTCTTCGGGAGCTAGCCAACTTGACACTAATACTTGGTAACAGAGATGACATTGAAGAAATGTCAAATAGTAGCTCGGTTGTTCTTACAACCGTACTCAAGCTCATTGACAAGTATGATCTGTATGGGCAAGTGGCCTATCCTAAGCATCACAAGCAATCAGATGTGCCAGACATTTATCGTCTAGCTGCAAAAACAAAGGGAGTTTTCATCAACCCAGCTCTCGTGGAACCTTTCGGGCTCACAATTATAGAGGCAGCTGCTTATGGTTTACCAGTGGTCGCCACCAAAAATGGAGGGCCTGTGGATATTCTGAAGGCACTAAATAACGGCCTCCTAGTTGACCCACATGACCAGAAAGCCATAGAAGAGGCACTGCTCAAACTTGTCGGAGAAAAAAACCTCTGGCTTGAATGCAGAAATAACGGTCTGAAGAACATCCACCGCTTTTCGTGGCCAGAACACTGCCGAAACTACCTCTCCCATGTTGAACATTCCAGAAACCGTCACCCCACTACCCGTCGTCAGATCACGCCAATTCCTGAAGAACCATTGAGTGACTCTCTAAAAGATGTGGAAGACCTTTCTTTGAGATTCTCTGTGGAAGGAGACTTCAAGCATAATGGAGAGCTCGATGCAGCAACTAGACAGAGGGAACTGATTGAAGCCATAACTAGAATGTCTTCGTCCACTAGCAATGTAGGTGCTACTTATGGTCCTGGCAGAAGGCAGAGTCTATTTGTAATTGCCATCGACTGTTATGACCAGAATGGAGACGGCACCCAGGTATTTCAAGAAATTCTAGTGAATGTAAAGAAAGCAGCAGGCTTAGGCTATGGCCAGGGTCGAGTAGGCATTGTGTTGTTAACTGGTTCCAGCTTACAAGATATCATGAAAGCGTTTAAAGGGTGCCAAGTAAACATAGAAGATTTTGACGCGTTGGTCTGCAAAAGTGGGAGTGAAATGTACTACCCATGGAGGGATTTAGCAGCTGATGCAGATTATGAAACCCATATCGAGTACAGATGGCCTGGGGAGAATGTGAGGTCCATGGTTCCAAGGCTAGCCAGGCTAGAAGTTGGAGCTGAGGATGATGTCGTGGAGTATGCCAGATCAAGTAGCTCTAGGTGCTACTCTTATAGTGTGAAACCAGGAGCCAAGACTCGACGGGTTGATGACCTGCGCCAATTGTTGCGGATGAGAGGCTTCCGATGCAACCTTGTCTACACTCGTGTAGCTTCAAGGTTAAATGTTGTACCCCTAGTTGCATCAAGAGTACAAGCACTAAGATATCTTTCTGTCAGGTGGGCCATTGATCTTTCGAAAGTGGTTGTATTTGTCGGGGAAAAAGGAGATACAGACAATGAAGACTTGCTGGCCGGCCTCCACAAGACCCTAGTTCTAAGAGGCTCTGTTGAGTATGGCAGTGAGAAGCTTATTCACGGTAAAGACAGTTTCAAAAGAGAAGATGTGGTCCCACAAGATAGCCTTAACATTGCCCTCGTGGAGAACTACCAAGCCCATGATATCTCAGCAGCACTTGAAGCACTTGGGATCAGGTGCTTTTAG

Protein Analysis

1025

Amino Acids

115.2

Weight (kDa)

6.76

Isoelectric Point (pI)

46.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_trans_4_4 PF13579 194 - 387 4.9e-08 Glycosyl transferase 4-like domain
Glycos_transf_1 PF00534 473 - 645 5e-24 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 477 - 632 1.6e-16 Glycosyl transferases group 1
S6PP PF05116 784 - 955 2.4e-19 Sucrose-6F-phosphate phosphohydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2447
AatII GACGTC 2 cut(s) 337, 1228
Acc16I TGCGCA 1 cut(s) 424
Acc36I ACCTGC 1 cut(s) 2694
AccB1I GGYRCC 3 cut(s) 354, 2285, 2415
AccB7I CCANNNNNTGG 1 cut(s) 731
AccI GTMKAC 3 cut(s) 639, 684, 2730
AccII CGCG 2 cut(s) 365, 2444
AciI CCGC 6 cut(s) 365, 582, 763, 1277, 1951, 2701
AclWI GGATC 3 cut(s) 913, 1100, 3068
AcoI YGGCCR 6 cut(s) 3, 847, 925, 1961, 2344, 2883
AcsI RAATTY 3 cut(s) 90, 827, 2304
AcuI CTGAAG 5 cut(s) 411, 1276, 1841, 1958, 2070
AcyI GRCGYC 3 cut(s) 334, 355, 1225
AdeI CACNNNGTG 1 cut(s) 856
AfaI GTAC 6 cut(s) 146, 1602, 2474, 2528, 2763, 2784
AfiI CCNNNNNNNGG 6 cut(s) 364, 731, 942, 1146, 1198, 2485
AflIII ACRYGT 1 cut(s) 2442
AjiI CACGTC 1 cut(s) 415
AjnI CCWGG 7 cut(s) 1279, 2224, 2289, 2346, 2536, 2573, 2656
Alw21I GWGCWC 4 cut(s) 619, 874, 1510, 2139
Alw26I GTCTC 5 cut(s) 655, 1216, 1452, 2109, 2274
AlwI GGATC 3 cut(s) 913, 1100, 3068
ApeKI GCWGC 9 cut(s) 741, 953, 1694, 1763, 1766, 2144, 2327, 2495, 3041
ApoI RAATTY 3 cut(s) 90, 827, 2304
Asp700I GAANNNNTTC 3 cut(s) 128, 568, 2188
AspLEI GCGC 4 cut(s) 267, 357, 425, 2691
AspS9I GGNCC 7 cut(s) 477, 776, 1805, 2222, 2553, 2814, 2980
AsuC2I CCSGG 1 cut(s) 967
AsuHPI GGTGA 6 cut(s) 666, 710, 890, 1112, 1129, 2006
AsuII TTCGAA 1 cut(s) 2828
AsuNHI GCTAGC 2 cut(s) 1519, 2569
AvaII GGWCC 5 cut(s) 477, 776, 2222, 2553, 2980
BalI TGGCCA 3 cut(s) 927, 1963, 2346
BanI GGYRCC 3 cut(s) 354, 2285, 2415
BanII GRGCYC 5 cut(s) 844, 874, 1510, 1749, 2139
BauI CACGAG 5 cut(s) 61, 612, 1730, 2736, 3008
BbsI GAAGAC 3 cut(s) 2091, 2181, 2880
Bbv12I GWGCWC 4 cut(s) 619, 874, 1510, 2139
BbvCI CCTCAGC 1 cut(s) 2592
BbvI GCAGC 9 cut(s) 753, 940, 1681, 1753, 1775, 2156, 2339, 2507, 3053
BccI CCATC 7 cut(s) 292, 725, 830, 1175, 1319, 2264, 2526
BceAI ACGGC 3 cut(s) 342, 1852, 2299
BciT130I CCWGG 7 cut(s) 1281, 2226, 2291, 2348, 2538, 2575, 2658
BclI TGATCA 1 cut(s) 519
BcnI CCSGG 1 cut(s) 967
BcoDI GTCTC 5 cut(s) 655, 1216, 1452, 2109, 2274
BfoI RGCGCY 1 cut(s) 358
BfuAI ACCTGC 1 cut(s) 2694
BglII AGATCT 1 cut(s) 1330
BisI GCNGC 9 cut(s) 742, 954, 1695, 1764, 1767, 2145, 2328, 2496, 3042
BlsI GCNGC 9 cut(s) 743, 955, 1696, 1765, 1768, 2146, 2329, 2497, 3043
BmcAI AGTACT 1 cut(s) 146
Bme1390I CCNGG 8 cut(s) 967, 1281, 2226, 2291, 2348, 2538, 2575, 2658
Bme18I GGWCC 5 cut(s) 477, 776, 2222, 2553, 2980
BmgBI CACGTC 1 cut(s) 415
BmgT120I GGNCC 7 cut(s) 477, 776, 1805, 2222, 2553, 2814, 2980
BmrFI CCNGG 8 cut(s) 967, 1281, 2226, 2291, 2348, 2538, 2575, 2658
BmrI ACTGGG 1 cut(s) 1005
BmtI GCTAGC 2 cut(s) 1523, 2573
BmuI ACTGGG 1 cut(s) 1005
BpiI GAAGAC 3 cut(s) 2091, 2181, 2880
BplI GAGNNNNNCTC 4 cut(s) 671, 703, 2053, 2085
BpmI CTGGAG 1 cut(s) 673
Bpu10I CCTNAGC 3 cut(s) 1650, 2335, 2592
Bpu14I TTCGAA 1 cut(s) 2828
BpuEI CTTGAG 4 cut(s) 269, 1178, 1469, 1589
BpuMI CCSGG 1 cut(s) 967
BsaAI YACGTR 1 cut(s) 258
BsaBI GATNNNNATC 1 cut(s) 1329
BsaHI GRCGYC 3 cut(s) 334, 355, 1225
BsaJI CCNNGG 9 cut(s) 922, 966, 1243, 2289, 2347, 2480, 2537, 2556, 2564
BsaWI WCCGGW 1 cut(s) 676
BsaXI ACNNNNNCTCC 8 cut(s) 1097, 1127, 1213, 1243, 1794, 1824, 1970, 2000
Bsc4I CCNNNNNNNGG 6 cut(s) 364, 731, 942, 1146, 1198, 2485
Bse118I RCCGGY 1 cut(s) 2885
Bse1I ACTGG 4 cut(s) 20, 1000, 1781, 2380
Bse3DI GCAATG 3 cut(s) 1172, 2209, 3000
Bse8I GATNNNNATC 1 cut(s) 1329
BseBI CCWGG 7 cut(s) 1281, 2226, 2291, 2348, 2538, 2575, 2658
BseDI CCNNGG 9 cut(s) 922, 966, 1243, 2289, 2347, 2480, 2537, 2556, 2564
BseGI GGATG 6 cut(s) 52, 313, 376, 1944, 2602, 2709
BseJI GATNNNNATC 1 cut(s) 1329
BseLI CCNNNNNNNGG 6 cut(s) 364, 731, 942, 1146, 1198, 2485
BseMI GCAATG 3 cut(s) 1172, 2209, 3000
BseMII CTCAG 3 cut(s) 1386, 2583, 3051
BseNI ACTGG 4 cut(s) 20, 1000, 1781, 2380
BseRI GAGGAG 2 cut(s) 1102, 1118
BseXI GCAGC 9 cut(s) 753, 940, 1681, 1753, 1775, 2156, 2339, 2507, 3053
BseYI CCCAGC 3 cut(s) 941, 1108, 1723
Bsh1236I CGCG 2 cut(s) 365, 2444
BshNI GGYRCC 3 cut(s) 354, 2285, 2415
BsiHKAI GWGCWC 4 cut(s) 619, 874, 1510, 2139
BsiSI CCGG 4 cut(s) 6, 677, 966, 2886
BslFI GGGAC 4 cut(s) 86, 425, 1185, 2966
BslI CCNNNNNNNGG 6 cut(s) 364, 731, 942, 1146, 1198, 2485
BsmAI GTCTC 5 cut(s) 655, 1216, 1452, 2109, 2274
BsmBI CGTCTC 2 cut(s) 1216, 2274
BsmFI GGGAC 4 cut(s) 86, 425, 1185, 2966
BsmI GAATGC 3 cut(s) 1000, 1505, 1925
Bsp119I TTCGAA 1 cut(s) 2828
Bsp1286I GDGCHC 6 cut(s) 619, 844, 874, 1510, 1749, 2139
Bsp19I CCATGG 3 cut(s) 922, 2480, 2556
BspACI CCGC 6 cut(s) 365, 582, 763, 1277, 1951, 2701
BspCNI CTCAG 3 cut(s) 1387, 2584, 3050
BspFNI CGCG 2 cut(s) 365, 2444
BspHI TCATGA 2 cut(s) 1083, 2397
BspMI ACCTGC 1 cut(s) 2694
BspOI GCTAGC 2 cut(s) 1523, 2573
BspPI GGATC 3 cut(s) 913, 1100, 3068
BspQI GCTCTTC 3 cut(s) 99, 1155, 1515
BspT104I TTCGAA 1 cut(s) 2828
BspT107I GGYRCC 3 cut(s) 354, 2285, 2415
BsrDI GCAATG 3 cut(s) 1172, 2209, 3000
BsrFI RCCGGY 1 cut(s) 2885
BsrI ACTGG 4 cut(s) 20, 1000, 1781, 2380
BssAI RCCGGY 1 cut(s) 2885
BssECI CCNNGG 9 cut(s) 922, 966, 1243, 2289, 2347, 2480, 2537, 2556, 2564
BssNI GRCGYC 3 cut(s) 334, 355, 1225
BssSI CACGAG 5 cut(s) 61, 612, 1730, 2736, 3008
BssT1I CCWWGG 5 cut(s) 922, 1243, 2480, 2556, 2564
Bst2BI CACGAG 5 cut(s) 61, 612, 1730, 2736, 3008
Bst2UI CCWGG 7 cut(s) 1281, 2226, 2291, 2348, 2538, 2575, 2658
Bst6I CTCTTC 6 cut(s) 62, 99, 559, 1155, 1515, 1869
BstACI GRCGYC 3 cut(s) 334, 355, 1225
BstAPI GCANNNNNTGC 1 cut(s) 2501
BstBAI YACGTR 1 cut(s) 258
BstBI TTCGAA 1 cut(s) 2828
BstC8I GCNNGC 6 cut(s) 263, 1521, 2332, 2571, 2883, 2887
BstDEI CTNAG 8 cut(s) 1211, 1395, 1650, 2335, 2592, 2792, 2909, 3037
BstDSI CCRYGG 3 cut(s) 922, 2480, 2556
BstEII GGTNACC 1 cut(s) 1135
BstF5I GGATG 6 cut(s) 52, 313, 376, 1944, 2602, 2709
BstFNI CGCG 2 cut(s) 365, 2444
BstH2I RGCGCY 1 cut(s) 358
BstHHI GCGC 4 cut(s) 267, 357, 425, 2691
BstMAI GTCTC 5 cut(s) 655, 1216, 1452, 2109, 2274
BstMWI GCNNNNNNNGC 9 cut(s) 271, 988, 997, 1660, 2501, 2575, 2634, 2718, 3050
BstNI CCWGG 7 cut(s) 1281, 2226, 2291, 2348, 2538, 2575, 2658
BstNSI RCATGY 2 cut(s) 469, 1258
BstPI GGTNACC 1 cut(s) 1135
BstSCI CCNGG 8 cut(s) 965, 1279, 2224, 2289, 2346, 2536, 2573, 2656
BstUI CGCG 2 cut(s) 365, 2444
BstV1I GCAGC 9 cut(s) 753, 940, 1681, 1753, 1775, 2156, 2339, 2507, 3053
BstV2I GAAGAC 3 cut(s) 2091, 2181, 2880
BstX2I RGATCY 1 cut(s) 1330
BstYI RGATCY 1 cut(s) 1330
BtgI CCRYGG 3 cut(s) 922, 2480, 2556
BtrI CACGTC 1 cut(s) 415
BtsCI GGATG 6 cut(s) 52, 313, 376, 1944, 2602, 2709
BtsI GCAGTG 3 cut(s) 1880, 1969, 2938
BtsIMutI CAGTG 5 cut(s) 441, 1788, 1880, 1969, 2938
BveI ACCTGC 1 cut(s) 2694
Cac8I GCNNGC 6 cut(s) 263, 1521, 2332, 2571, 2883, 2887
CciI TCATGA 2 cut(s) 1083, 2397
CfoI GCGC 4 cut(s) 267, 357, 425, 2691
Cfr10I RCCGGY 1 cut(s) 2885
Cfr13I GGNCC 7 cut(s) 477, 776, 1805, 2222, 2553, 2814, 2980
CseI GACGC 3 cut(s) 320, 371, 2450
Csp6I GTAC 6 cut(s) 145, 1601, 2473, 2527, 2762, 2783
CspCI CAANNNNNGTGG 4 cut(s) 1248, 1283, 1420, 1455
CviQI GTAC 6 cut(s) 145, 1601, 2473, 2527, 2762, 2783
DdeI CTNAG 8 cut(s) 1211, 1395, 1650, 2335, 2592, 2792, 2909, 3037
DinI GGCGCC 1 cut(s) 356
DraI TTTAAA 1 cut(s) 2410
DraIII CACNNNGTG 1 cut(s) 856
DrdI GACNNNNNNGTC 1 cut(s) 2447
DseDI GACNNNNNNGTC 1 cut(s) 2447
EaeI YGGCCR 6 cut(s) 3, 847, 925, 1961, 2344, 2883
Eam1104I CTCTTC 6 cut(s) 62, 99, 559, 1155, 1515, 1869
EarI CTCTTC 6 cut(s) 62, 99, 559, 1155, 1515, 1869
EciI GGCGGA 1 cut(s) 752
Ecl136II GAGCTC 3 cut(s) 872, 1508, 2137
Eco130I CCWWGG 5 cut(s) 922, 1243, 2480, 2556, 2564
Eco147I AGGCCT 1 cut(s) 1490
Eco24I GRGCYC 5 cut(s) 844, 874, 1510, 1749, 2139
Eco32I GATATC 3 cut(s) 2395, 2798, 3034
Eco47I GGWCC 5 cut(s) 477, 776, 2222, 2553, 2980
Eco53kI GAGCTC 3 cut(s) 872, 1508, 2137
Eco57I CTGAAG 5 cut(s) 411, 1276, 1841, 1958, 2070
Eco91I GGTNACC 1 cut(s) 1135
EcoICRI GAGCTC 3 cut(s) 872, 1508, 2137
EcoO109I RGGNCCY 1 cut(s) 1805
EcoO65I GGTNACC 1 cut(s) 1135
EcoRI GAATTC 1 cut(s) 827
EcoRII CCWGG 7 cut(s) 1279, 2224, 2289, 2346, 2536, 2573, 2656
EcoRV GATATC 3 cut(s) 2395, 2798, 3034
EcoT14I CCWWGG 5 cut(s) 922, 1243, 2480, 2556, 2564
EcoT22I ATGCAT 1 cut(s) 531
EcoT38I GRGCYC 5 cut(s) 844, 874, 1510, 1749, 2139
EgeI GGCGCC 1 cut(s) 356
EheI GGCGCC 1 cut(s) 356
ErhI CCWWGG 5 cut(s) 922, 1243, 2480, 2556, 2564
Esp3I CGTCTC 2 cut(s) 1216, 2274
FalI AAGNNNNNCTT 4 cut(s) 792, 824, 2979, 3011
FaqI GGGAC 4 cut(s) 86, 425, 1185, 2966
FauNDI CATATG 1 cut(s) 719
FbaI TGATCA 1 cut(s) 519
FblI GTMKAC 3 cut(s) 639, 684, 2730
Fnu4HI GCNGC 9 cut(s) 742, 954, 1695, 1764, 1767, 2145, 2328, 2496, 3042
FokI GGATG 6 cut(s) 59, 320, 383, 1931, 2609, 2716
FriOI GRGCYC 5 cut(s) 844, 874, 1510, 1749, 2139
FseI GGCCGGCC 1 cut(s) 2889
Fsp4HI GCNGC 9 cut(s) 742, 954, 1695, 1764, 1767, 2145, 2328, 2496, 3042
FspI TGCGCA 1 cut(s) 424
GlaI GCGC 4 cut(s) 266, 356, 424, 2690
GluI GCNGC 9 cut(s) 742, 954, 1695, 1764, 1767, 2145, 2328, 2496, 3042
GsaI CCCAGC 3 cut(s) 945, 1112, 1727
GsuI CTGGAG 1 cut(s) 673
HaeII RGCGCY 1 cut(s) 358
HapII CCGG 4 cut(s) 6, 677, 966, 2886
HgaI GACGC 3 cut(s) 320, 371, 2450
HhaI GCGC 4 cut(s) 267, 357, 425, 2691
Hin1I GRCGYC 3 cut(s) 334, 355, 1225
Hin6I GCGC 4 cut(s) 265, 355, 423, 2689
HinP1I GCGC 4 cut(s) 265, 355, 423, 2689
HincII GTYRAC 4 cut(s) 490, 1050, 1849, 2373
HindII GTYRAC 4 cut(s) 490, 1050, 1849, 2373
HindIII AAGCTT 1 cut(s) 2939
HpaI GTTAAC 2 cut(s) 490, 2373
HpaII CCGG 4 cut(s) 6, 677, 966, 2886
HphI GGTGA 6 cut(s) 666, 710, 890, 1112, 1129, 2006
Hpy99I CGWCG 5 cut(s) 80, 336, 1230, 2031, 2676
HpyAV CCTTC 7 cut(s) 386, 394, 433, 1313, 1816, 2105, 2226
HpyCH4IV ACGT 6 cut(s) 257, 334, 414, 510, 978, 1225
HpyF10VI GCNNNNNNNGC 9 cut(s) 271, 988, 997, 1660, 2501, 2575, 2634, 2718, 3050
HpyF3I CTNAG 8 cut(s) 1211, 1395, 1650, 2335, 2592, 2792, 2909, 3037
HpySE526I ACGT 6 cut(s) 257, 334, 414, 510, 978, 1225
Hsp92I GRCGYC 3 cut(s) 334, 355, 1225
HspAI GCGC 4 cut(s) 265, 355, 423, 2689
KasI GGCGCC 1 cut(s) 354
KroI GCCGGC 1 cut(s) 2885
KroNI GCCGGC 1 cut(s) 2887
Ksp22I TGATCA 1 cut(s) 519
KspAI GTTAAC 2 cut(s) 490, 2373
LguI GCTCTTC 3 cut(s) 99, 1155, 1515
LmnI GCTCC 6 cut(s) 346, 749, 1105, 1516, 2588, 2660
Lsp1109I GCAGC 9 cut(s) 753, 940, 1681, 1753, 1775, 2156, 2339, 2507, 3053
MaeII ACGT 6 cut(s) 257, 334, 414, 510, 978, 1225
MaeIII GTNAC 8 cut(s) 359, 804, 1135, 1300, 1471, 1544, 2012, 2063
MfeI CAATTG 2 cut(s) 1028, 2693
MflI RGATCY 1 cut(s) 1330
MhlI GDGCHC 6 cut(s) 619, 844, 874, 1510, 1749, 2139
MlsI TGGCCA 3 cut(s) 927, 1963, 2346
MluI ACGCGT 1 cut(s) 2442
MluNI TGGCCA 3 cut(s) 927, 1963, 2346
Mly113I GGCGCC 1 cut(s) 355
MlyI GAGTC 9 cut(s) 188, 645, 680, 812, 1167, 1242, 2060, 2248, 2662
MmeI TCCRAC 6 cut(s) 161, 486, 1095, 1179, 1876, 2566
Mox20I TGGCCA 3 cut(s) 927, 1963, 2346
Mph1103I ATGCAT 1 cut(s) 531
MroNI GCCGGC 1 cut(s) 2885
MroXI GAANNNNTTC 3 cut(s) 128, 568, 2188
MscI TGGCCA 3 cut(s) 927, 1963, 2346
MseI TTAA 8 cut(s) 20, 204, 449, 489, 2372, 2409, 2753, 2997
MslI CAYNNNNRTG 1 cut(s) 470
Msp20I TGGCCA 3 cut(s) 927, 1963, 2346
MspA1I CMGCKG 4 cut(s) 744, 1296, 1766, 2498
MspI CCGG 4 cut(s) 6, 677, 966, 2886
MspR9I CCNGG 8 cut(s) 967, 1281, 2226, 2291, 2348, 2538, 2575, 2658
MunI CAATTG 2 cut(s) 1028, 2693
Mva1269I GAATGC 3 cut(s) 1000, 1505, 1925
MvaI CCWGG 7 cut(s) 1281, 2226, 2291, 2348, 2538, 2575, 2658
MvnI CGCG 2 cut(s) 365, 2444
MwoI GCNNNNNNNGC 9 cut(s) 271, 988, 997, 1660, 2501, 2575, 2634, 2718, 3050
NaeI GCCGGC 1 cut(s) 2887
NarI GGCGCC 1 cut(s) 355
NciI CCSGG 1 cut(s) 967
NcoI CCATGG 3 cut(s) 922, 2480, 2556
NdeI CATATG 1 cut(s) 719
NgoMIV GCCGGC 1 cut(s) 2885
NheI GCTAGC 2 cut(s) 1519, 2569
NmeAIII GCCGAG 4 cut(s) 145, 254, 825, 1529
NmuCI GTSAC 5 cut(s) 359, 804, 1135, 2012, 2063
NsbI TGCGCA 1 cut(s) 424
NsiI ATGCAT 1 cut(s) 531
NspI RCATGY 2 cut(s) 469, 1258
NspV TTCGAA 1 cut(s) 2828
PagI TCATGA 2 cut(s) 1083, 2397
PceI AGGCCT 1 cut(s) 1490
PciSI GCTCTTC 3 cut(s) 99, 1155, 1515
PctI GAATGC 3 cut(s) 1000, 1505, 1925
PdiI GCCGGC 1 cut(s) 2887
PdmI GAANNNNTTC 3 cut(s) 128, 568, 2188
PfeI GAWTC 4 cut(s) 569, 1118, 1313, 2100
PflMI CCANNNNNTGG 1 cut(s) 731
PkrI GCNGC 9 cut(s) 743, 955, 1696, 1765, 1768, 2146, 2329, 2497, 3043
PleI GAGTC 9 cut(s) 187, 644, 680, 811, 1167, 1241, 2060, 2247, 2662
PluTI GGCGCC 1 cut(s) 358
PpsI GAGTC 9 cut(s) 187, 644, 680, 811, 1167, 1241, 2060, 2247, 2662
Ppu21I YACGTR 1 cut(s) 258
Psp124BI GAGCTC 3 cut(s) 874, 1510, 2139
Psp6I CCWGG 7 cut(s) 1279, 2224, 2289, 2346, 2536, 2573, 2656
PspEI GGTNACC 1 cut(s) 1135
PspFI CCCAGC 3 cut(s) 941, 1108, 1723
PspGI CCWGG 7 cut(s) 1279, 2224, 2289, 2346, 2536, 2573, 2656
PspPI GGNCC 7 cut(s) 477, 776, 1805, 2222, 2553, 2814, 2980
PsuI RGATCY 1 cut(s) 1330
PvuII CAGCTG 4 cut(s) 744, 1296, 1766, 2498
RigI GGCCGGCC 1 cut(s) 2889
RsaI GTAC 6 cut(s) 146, 1602, 2474, 2528, 2763, 2784
RsaNI GTAC 6 cut(s) 145, 1601, 2473, 2527, 2762, 2783
RseI CAYNNNNRTG 1 cut(s) 470
SacI GAGCTC 3 cut(s) 874, 1510, 2139
SapI GCTCTTC 3 cut(s) 99, 1155, 1515
SaqAI TTAA 8 cut(s) 20, 204, 449, 489, 2372, 2409, 2753, 2997
SatI GCNGC 9 cut(s) 742, 954, 1695, 1764, 1767, 2145, 2328, 2496, 3042
Sau96I GGNCC 7 cut(s) 477, 776, 1805, 2222, 2553, 2814, 2980
ScaI AGTACT 1 cut(s) 146
SchI GAGTC 9 cut(s) 188, 645, 680, 812, 1167, 1242, 2060, 2248, 2662
ScrFI CCNGG 8 cut(s) 967, 1281, 2226, 2291, 2348, 2538, 2575, 2658
SduI GDGCHC 6 cut(s) 619, 844, 874, 1510, 1749, 2139
SfoI GGCGCC 1 cut(s) 356
SfuI TTCGAA 1 cut(s) 2828
SinI GGWCC 5 cut(s) 477, 776, 2222, 2553, 2980
SmiMI CAYNNNNRTG 1 cut(s) 470
SmlI CTYRAG 4 cut(s) 248, 1193, 1484, 1604
SmoI CTYRAG 4 cut(s) 248, 1193, 1484, 1604
SseBI AGGCCT 1 cut(s) 1490
SsiI CCGC 6 cut(s) 365, 582, 763, 1277, 1951, 2701
SspDI GGCGCC 1 cut(s) 354
SstI GAGCTC 3 cut(s) 874, 1510, 2139
StuI AGGCCT 1 cut(s) 1490
StyD4I CCNGG 8 cut(s) 965, 1279, 2224, 2289, 2346, 2536, 2573, 2656
StyI CCWWGG 5 cut(s) 922, 1243, 2480, 2556, 2564
TaiI ACGT 6 cut(s) 260, 337, 417, 513, 981, 1228
TaqI TCGA 8 cut(s) 1095, 1158, 2139, 2259, 2353, 2523, 2671, 2828
TatI WGTACW 4 cut(s) 144, 2472, 2526, 2782
TfiI GAWTC 4 cut(s) 569, 1118, 1313, 2100
Tru1I TTAA 8 cut(s) 20, 204, 449, 489, 2372, 2409, 2753, 2997
Tru9I TTAA 8 cut(s) 20, 204, 449, 489, 2372, 2409, 2753, 2997
TscAI CASTG 5 cut(s) 441, 1788, 1887, 1976, 2938
TseFI GTSAC 5 cut(s) 359, 804, 1135, 2012, 2063
TseI GCWGC 9 cut(s) 741, 953, 1694, 1763, 1766, 2144, 2327, 2495, 3041
Tsp45I GTSAC 5 cut(s) 359, 804, 1135, 2012, 2063
TspDTI ATGAA 4 cut(s) 1705, 2414, 2526, 2886
TspRI CASTG 5 cut(s) 441, 1788, 1887, 1976, 2938
Van91I CCANNNNNTGG 1 cut(s) 731
VpaK11BI GGWCC 5 cut(s) 477, 776, 2222, 2553, 2980
XapI RAATTY 3 cut(s) 90, 827, 2304
XceI RCATGY 2 cut(s) 469, 1258
XmiI GTMKAC 3 cut(s) 639, 684, 2730
XmnI GAANNNNTTC 3 cut(s) 128, 568, 2188
ZraI GACGTC 2 cut(s) 335, 1226
ZrmI AGTACT 1 cut(s) 146
Zsp2I ATGCAT 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.