Rroxscaffold_7G00217470

Belongs to the glycosyltransferase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0006699
Physical Location & Seq
Forward (+)
67945620 .. 67951669
6050 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00217470.1

Sequence Viewer

Length: 3006 bp
ATGGCGGGAAACGACTGGTTAAACGGTTATCTCGAGGCGATCCTGGATGCAGGAATTAACACAAGGAAAACGAACGATGGGAAGCAGAAGATTGCCAAGTTTGAAGAGCAAGTCAAGGAGGAGAAATTGTTTAGTCCCACAAAGTACTTCGTGGAAGAAGTGGTTAGCAGCTTTGATGAGTCTGACCTCCACAGGACGTGGGTTAAGGTAGTAGCTACAAGGAATACCCGTGAACGCAGCAATAGGCTTGAGAATATGTGCTGGCGCATTTGGCATCTCGCTCGTAAGAAGAAACAGATAGCATGGGATGATGCTCGCAGACTTGCAAGACGACGTCTGGAGAGGGAGCAAGGCCGCCATGATGCTGCGGAGGACCTTTCCGAGCTGTCTGAAGGGGAGAAGGAAAAAGGTGACACCAATTTTACAGAACCGCCTGTCAAGCAAATTGCAAGAATCAACTCAGAAATGCGATTGTGGTCTGAGGATGACAACAAGACTAGGCATCTTTACATTGTCTTAATCAGTATGCATGGGTTGGTTCGTGGAGAAAACATGGAACTTGGAAGAGATTCTGATACAGGTGGTCAGGTGAAGTATGTAGTGGAACTTGCTCGTGCTCTGGCCAATACAAAAGGAGTCTATAGAGTTGATCTGTTAACAAGGCAAATCACTTCACCGGAGGTAGACTCCAGCTACGGTGAACCTAATGAGATGCTCATTTGCCCACCTGATGGCAGCGGGAGCTGTGGAGCCTACATTGTCCGCCTTCCATGTGGACCCCGCGACAAGTACATACCGAAAGAGTCACTATGGCCTCACATACCTGAATTTATTGATGGGGCTCTCGGCCACATTGTGAACATGGCAAGAGCTCTAGGCGATGAGGTCAATGGTGGGAAACCAACATGGCCTTATGTCATCCACGGCCACTATGCTGATGCAGGGGAGGTGGCTGCTCAGTTGTCTGGTGCCTTGAATGTGCCAATGGTGCTGACCGGGCATTCCCTAGGAAGAAACAAGTTTGAACAACTGCTCAAACAGGGGAGATTGTCGAGGGAGGACATCAATGCAACCTACAAGATTATGAAGAGGATCGAGGCTGAGGAGCTGGCTTTGGATGCTGCTGAAATGGTGGTGACTAGTACTAGGCAAGAGATTGAAGAGCAGTGGGGACTGTATGATGGGTTCGATCTCAAGTTGGAGAGGAAGCTCAGGGTTAGGAGACGCCGGGGAGTTAGTTGCCTTGGACGATACATGCCAAGAATGGTGGTTATACCGCCAGGCATGGACTTCAGCTATGTTACAGTTCAAGATACTGAAGGAGATGGAGATCTCAAATCCCTGATTGGTTCTGACAGAGCTCAAAGCAAAAGGAATCTCCCTCCAATATGGTCTGAGGTAATGCGATTTTTCACTAATCCTCACAAGCCCACTATACTTGCATTGTCTCGTCCTGACCCAAAGAAAAATGTCACTACATTGCTGAAGGCCTTTGGGGAATGCAAACCGCTTCGGGAGCTAGCCAACCTGGCACTGATACTTGGTAACAGAGATGACATTGAAGAAATGCCAAATAGCAGCTCGGTTGTTCTAACAACTGTGCTCAAGCTGATCGACAGGTATGATCTGTATGGTCAAGTGGCCTATCCTAAACATCACAAGCAATCAGATGTCCCTGACATATATCGTCTAGCTGCAAAAACAAAGGGAGTGTTTATCAACCCAGCTCTTGTGGAACCTTTCGGTCTCACTATTATAGAGGCAGCTGCTTATGGTTTACCAGTGGTTGCCACCAGAAACGGAGGACCTGTGGACATTCTGAAGGCACTAAACAATGGCGTCCTAGTTGACCCACATGACCAGAAGGCCATAGAAGAAGCACTGCTAAAGCTTGTTGCAGATAAAAACCTCTGGTTGGAATGCAGAAAGAATGGCCTAAAGAACATCCACCGCTTTTCGTGGCCAGAACACTGCCGAAACTACCTCTCCCATGTTGAACACTCCCGAAACCGCCACCCTACCGCCCGCATTCAGATCGTACCAATTCCTGAAGAACCAATGAGTGACTCTCTAAAAGACGTGGATGACCTTTCTTTGAGATTCTCTGTAGACGGAGATTTCAAGCATAATACCAATGCTGGTGCTACTTACTGTCCTGGTAGGAGGCAGAGGCTGTTTGTAATAGCCGTGGACTGTTATGACCAGAATGGAGATGGTACTCAGACGTTTCAGGAAATTATAACGAGTGTCAAGAAAGCAGCAAGCTTAGGATTTGGCCAGGGTCGTGTAGGCTTCGTATTGTTAACTGGTTCCAGCTTACAAGAAACCGTGAAGGCATTTAAAGGGTGCCAAGTCAGCATAGAAGAATTTGATGCGGTGGTCTGCAAAAGTGGCAGTGAAATGTATTACCCATGGAGAGATTTGGCAGCTGATGCAGATTTTGAAAGTCATATCGAGTACAGATGGCCTGGAGAGAATGTGAGGTCCATGGTGCCAAGGCTGGCCATTTTAGAAGGTGGGGCTGAGGATGACATCACGGAGTATGGAGGATCAAGTAGCTCAAGGTGCTACTCTTATAATGTGAAACCAGGAGCCAAGACTCGGCGAATTGATGACCTGCGCCAAAGGCTGCGGATGCGAGGCTTCCGATGCAACCTTTTCTACACTCGTGCAGCATCAAGGTTAAATGTTGTGCCACTCTTTGCATCGAGAGTACAAGCACTGAGATATCTTTCGGTCAGGTGGGGTACTGATTTATCCAAAGTGGTTATGTTTGTGGGGGAAAAAGGAGATACAGATAATGAAGACCTGCTGGCTGGTCTCCATAAGACACTAGTTTTAAGAGGATCCGTTGAGTATGGCAGTGAGAAGCTTCTTCATAGTGAAGATGGTTTCAGAAGAGATGATGTAGTCCCACAAGATAGCCCCAACATTGCCCTTGTAGAGAGCTATCAACCCCACGATATCTCAGCCACTCTTGAAGCTCTTGGGATAAAGGGACTTTAG

Protein Analysis

1001

Amino Acids

112.77

Weight (kDa)

6.81

Isoelectric Point (pI)

45.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_trans_4_4 PF13579 194 - 387 1.5e-07 Glycosyl transferase 4-like domain
GT-B_Sucrose_synth PF00862 263 - 432 2.9e-06 Sucrose synthase first GT-B domain
Glycos_transf_1 PF00534 473 - 646 2.5e-24 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 477 - 634 1.6e-16 Glycosyl transferases group 1
S6PP PF05116 761 - 932 2.7e-17 Sucrose-6F-phosphate phosphohydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2240, 2577
AatII GACGTC 1 cut(s) 337
Acc36I ACCTGC 2 cut(s) 2625, 2817
AccB1I GGYRCC 3 cut(s) 968, 2346, 2491
AccB7I CCANNNNNTGG 1 cut(s) 731
AccI GTMKAC 2 cut(s) 684, 2109
AccII CGCG 1 cut(s) 783
AclWI GGATC 5 cut(s) 34, 1100, 2557, 2841, 2854
AcoI YGGCCR 6 cut(s) 621, 847, 925, 1961, 2275, 2502
AcsI RAATTY 2 cut(s) 827, 2366
AcuI CTGAAG 6 cut(s) 411, 1276, 1338, 1505, 1841, 2070
AcyI GRCGYC 3 cut(s) 334, 1225, 1839
AdeI CACNNNGTG 1 cut(s) 856
AfaI GTAC 8 cut(s) 146, 791, 1144, 2040, 2218, 2459, 2715, 2749
AfiI CCNNNNNNNGG 3 cut(s) 731, 1915, 2601
AhlI ACTAGT 2 cut(s) 1139, 2833
AjiI CACGTC 2 cut(s) 198, 2080
AjnI CCWGG 7 cut(s) 42, 1279, 1527, 2155, 2277, 2467, 2587
Alw21I GWGCWC 4 cut(s) 619, 874, 1363, 1605
Alw26I GTCTC 4 cut(s) 1216, 1452, 1751, 2825
AlwI GGATC 5 cut(s) 34, 1100, 2557, 2841, 2854
AlwNI CAGNNNCTG 1 cut(s) 2173
Ama87I CYCGRG 1 cut(s) 32
ApoI RAATTY 2 cut(s) 827, 2366
Asp700I GAANNNNTTC 1 cut(s) 568
AspA2I CCTAGG 1 cut(s) 1006
AspLEI GCGC 2 cut(s) 267, 2622
AspS9I GGNCC 4 cut(s) 373, 776, 1805, 2484
AsuC2I CCSGG 2 cut(s) 997, 1229
AsuHPI GGTGA 5 cut(s) 422, 601, 666, 710, 1147
AsuNHI GCTAGC 1 cut(s) 1519
AvaI CYCGRG 1 cut(s) 32
AvaII GGWCC 4 cut(s) 373, 776, 1805, 2484
AvrII CCTAGG 1 cut(s) 1006
BalI TGGCCA 4 cut(s) 623, 1963, 2277, 2504
BamHI GGATCC 1 cut(s) 2846
BanI GGYRCC 3 cut(s) 968, 2346, 2491
BanII GRGCYC 3 cut(s) 844, 874, 1363
BauI CACGAG 2 cut(s) 612, 2667
BbsI GAAGAC 1 cut(s) 2811
Bbv12I GWGCWC 4 cut(s) 619, 874, 1363, 1605
BbvCI CCTCAGC 2 cut(s) 1101, 2523
BccI CCATC 8 cut(s) 71, 725, 830, 1175, 1319, 2207, 2457, 2882
BceAI ACGGC 2 cut(s) 940, 2171
BcgI CGANNNNNNTGC 4 cut(s) 263, 297, 2017, 2051
BciT130I CCWGG 7 cut(s) 44, 1281, 1529, 2157, 2279, 2469, 2589
BcnI CCSGG 2 cut(s) 997, 1229
BcoDI GTCTC 4 cut(s) 1216, 1452, 1751, 2825
BcuI ACTAGT 2 cut(s) 1139, 2833
BfaI CTAG 9 cut(s) 498, 875, 1007, 1140, 1146, 1520, 1691, 1844, 2834
BfmI CTRYAG 2 cut(s) 640, 2106
BfuAI ACCTGC 2 cut(s) 2625, 2817
BglI GCCNNNNNGGC 1 cut(s) 1529
BglII AGATCT 1 cut(s) 1330
BlnI CCTAGG 1 cut(s) 1006
BmcAI AGTACT 2 cut(s) 146, 1144
Bme1390I CCNGG 9 cut(s) 44, 997, 1229, 1281, 1529, 2157, 2279, 2469, 2589
Bme18I GGWCC 4 cut(s) 373, 776, 1805, 2484
BmeT110I CYCGRG 1 cut(s) 32
BmgBI CACGTC 2 cut(s) 198, 2080
BmgT120I GGNCC 4 cut(s) 373, 776, 1805, 2484
BmiI GGNNCC 9 cut(s) 751, 778, 970, 1737, 2311, 2348, 2493, 2593, 2848
BmrFI CCNGG 9 cut(s) 44, 997, 1229, 1281, 1529, 2157, 2279, 2469, 2589
BmtI GCTAGC 1 cut(s) 1523
BpiI GAAGAC 1 cut(s) 2811
BplI GAGNNNNNCTC 4 cut(s) 671, 703, 2053, 2085
BpmI CTGGAG 3 cut(s) 359, 673, 2490
Bpu10I CCTNAGC 4 cut(s) 1101, 1211, 2266, 2523
BpuEI CTTGAG 4 cut(s) 269, 1178, 1589, 2545
BpuMI CCSGG 2 cut(s) 997, 1229
BsaBI GATNNNNATC 1 cut(s) 1329
BsaHI GRCGYC 3 cut(s) 334, 1225, 1839
BsaI GGTCTC 2 cut(s) 1751, 2825
BsaJI CCNNGG 9 cut(s) 922, 1006, 1228, 1243, 2187, 2278, 2411, 2487, 2495
BsaWI WCCGGW 1 cut(s) 676
BsaXI ACNNNNNCTCC 8 cut(s) 113, 143, 1794, 1824, 1970, 2000, 2463, 2493
Bsc4I CCNNNNNNNGG 3 cut(s) 731, 1915, 2601
Bse1I ACTGG 3 cut(s) 20, 1781, 2311
Bse3DI GCAATG 2 cut(s) 1478, 2931
Bse8I GATNNNNATC 1 cut(s) 1329
BseBI CCWGG 7 cut(s) 44, 1281, 1529, 2157, 2279, 2469, 2589
BseDI CCNNGG 9 cut(s) 922, 1006, 1228, 1243, 2187, 2278, 2411, 2487, 2495
BseGI GGATG 9 cut(s) 52, 313, 490, 918, 1123, 1944, 2089, 2533, 2640
BseJI GATNNNNATC 1 cut(s) 1329
BseLI CCNNNNNNNGG 3 cut(s) 731, 1915, 2601
BseMI GCAATG 2 cut(s) 1478, 2931
BseNI ACTGG 3 cut(s) 20, 1781, 2311
BseRI GAGGAG 2 cut(s) 134, 1118
BseYI CCCAGC 1 cut(s) 1723
BsgI GTGCAG 1 cut(s) 2691
Bsh1236I CGCG 1 cut(s) 783
BshNI GGYRCC 3 cut(s) 968, 2346, 2491
BsiHKAI GWGCWC 4 cut(s) 619, 874, 1363, 1605
BsiHKCI CYCGRG 1 cut(s) 32
BsiSI CCGG 3 cut(s) 677, 996, 1228
BslFI GGGAC 4 cut(s) 120, 1185, 1658, 2897
BslI CCNNNNNNNGG 3 cut(s) 731, 1915, 2601
BsmAI GTCTC 4 cut(s) 1216, 1452, 1751, 2825
BsmBI CGTCTC 1 cut(s) 1216
BsmFI GGGAC 4 cut(s) 120, 1185, 1658, 2897
BsmI GAATGC 4 cut(s) 1000, 1505, 1925, 2028
Bso31I GGTCTC 2 cut(s) 1751, 2825
BsoBI CYCGRG 1 cut(s) 32
Bsp1286I GDGCHC 5 cut(s) 619, 844, 874, 1363, 1605
Bsp19I CCATGG 2 cut(s) 2411, 2487
BspFNI CGCG 1 cut(s) 783
BspLI GGNNCC 9 cut(s) 751, 778, 970, 1737, 2311, 2348, 2493, 2593, 2848
BspMI ACCTGC 2 cut(s) 2625, 2817
BspOI GCTAGC 1 cut(s) 1523
BspPI GGATC 5 cut(s) 34, 1100, 2557, 2841, 2854
BspQI GCTCTTC 2 cut(s) 99, 1155
BspT107I GGYRCC 3 cut(s) 968, 2346, 2491
BspTNI GGTCTC 2 cut(s) 1751, 2825
BsrDI GCAATG 2 cut(s) 1478, 2931
BsrI ACTGG 3 cut(s) 20, 1781, 2311
BssECI CCNNGG 9 cut(s) 922, 1006, 1228, 1243, 2187, 2278, 2411, 2487, 2495
BssNI GRCGYC 3 cut(s) 334, 1225, 1839
BssSI CACGAG 2 cut(s) 612, 2667
BssT1I CCWWGG 5 cut(s) 1006, 1243, 2411, 2487, 2495
Bst2BI CACGAG 2 cut(s) 612, 2667
Bst2UI CCWGG 7 cut(s) 44, 1281, 1529, 2157, 2279, 2469, 2589
Bst4CI ACNGT 8 cut(s) 26, 698, 1176, 1306, 1600, 2153, 2195, 2329
Bst6I CTCTTC 5 cut(s) 99, 559, 1082, 1155, 2893
BstACI GRCGYC 3 cut(s) 334, 1225, 1839
BstAPI GCANNNNNTGC 1 cut(s) 2432
BstC8I GCNNGC 8 cut(s) 263, 316, 1110, 1521, 2026, 2263, 2502, 2814
BstDSI CCRYGG 4 cut(s) 922, 2187, 2411, 2487
BstF5I GGATG 9 cut(s) 52, 313, 490, 918, 1123, 1944, 2089, 2533, 2640
BstFNI CGCG 1 cut(s) 783
BstHHI GCGC 2 cut(s) 267, 2622
BstMAI GTCTC 4 cut(s) 1216, 1452, 1751, 2825
BstNI CCWGG 7 cut(s) 44, 1281, 1529, 2157, 2279, 2469, 2589
BstNSI RCATGY 1 cut(s) 1258
BstSCI CCNGG 9 cut(s) 42, 995, 1227, 1279, 1527, 2155, 2277, 2467, 2587
BstSFI CTRYAG 2 cut(s) 640, 2106
BstUI CGCG 1 cut(s) 783
BstV2I GAAGAC 1 cut(s) 2811
BstX2I RGATCY 2 cut(s) 1330, 2846
BstYI RGATCY 2 cut(s) 1330, 2846
BtgI CCRYGG 4 cut(s) 922, 2187, 2411, 2487
BtgZI GCGATG 1 cut(s) 894
BtrI CACGTC 2 cut(s) 198, 2080
BtsCI GGATG 9 cut(s) 52, 313, 490, 918, 1123, 1944, 2089, 2533, 2640
BtsI GCAGTG 5 cut(s) 1172, 1880, 1969, 2401, 2869
BtsIMutI CAGTG 8 cut(s) 1172, 1532, 1788, 1880, 1969, 2401, 2720, 2869
BveI ACCTGC 2 cut(s) 2625, 2817
Cac8I GCNNGC 8 cut(s) 263, 316, 1110, 1521, 2026, 2263, 2502, 2814
CaiI CAGNNNCTG 1 cut(s) 2173
CfoI GCGC 2 cut(s) 267, 2622
Cfr13I GGNCC 4 cut(s) 373, 776, 1805, 2484
CseI GACGC 2 cut(s) 1233, 1828
Csp6I GTAC 8 cut(s) 145, 790, 1143, 2039, 2217, 2458, 2714, 2748
CspCI CAANNNNNGTGG 4 cut(s) 1248, 1283, 1420, 1455
CviQI GTAC 8 cut(s) 145, 790, 1143, 2039, 2217, 2458, 2714, 2748
DraI TTTAAA 1 cut(s) 2341
DraIII CACNNNGTG 1 cut(s) 856
EaeI YGGCCR 6 cut(s) 621, 847, 925, 1961, 2275, 2502
Eam1104I CTCTTC 5 cut(s) 99, 559, 1082, 1155, 2893
EarI CTCTTC 5 cut(s) 99, 559, 1082, 1155, 2893
EciI GGCGGA 1 cut(s) 752
Ecl136II GAGCTC 2 cut(s) 872, 1361
Eco130I CCWWGG 5 cut(s) 1006, 1243, 2411, 2487, 2495
Eco147I AGGCCT 1 cut(s) 1490
Eco24I GRGCYC 3 cut(s) 844, 874, 1363
Eco31I GGTCTC 2 cut(s) 1751, 2825
Eco32I GATATC 2 cut(s) 2729, 2965
Eco47I GGWCC 4 cut(s) 373, 776, 1805, 2484
Eco53kI GAGCTC 2 cut(s) 872, 1361
Eco57I CTGAAG 6 cut(s) 411, 1276, 1338, 1505, 1841, 2070
Eco88I CYCGRG 1 cut(s) 32
EcoICRI GAGCTC 2 cut(s) 872, 1361
EcoO109I RGGNCCY 2 cut(s) 373, 1805
EcoRII CCWGG 7 cut(s) 42, 1279, 1527, 2155, 2277, 2467, 2587
EcoRV GATATC 2 cut(s) 2729, 2965
EcoT14I CCWWGG 5 cut(s) 1006, 1243, 2411, 2487, 2495
EcoT22I ATGCAT 1 cut(s) 531
EcoT38I GRGCYC 3 cut(s) 844, 874, 1363
ErhI CCWWGG 5 cut(s) 1006, 1243, 2411, 2487, 2495
Esp3I CGTCTC 1 cut(s) 1216
FaqI GGGAC 4 cut(s) 120, 1185, 1658, 2897
FauI CCCGC 3 cut(s) 731, 788, 2033
FblI GTMKAC 2 cut(s) 684, 2109
FokI GGATG 9 cut(s) 59, 320, 497, 905, 1130, 1931, 2096, 2540, 2647
FriOI GRGCYC 3 cut(s) 844, 874, 1363
FspBI CTAG 9 cut(s) 498, 875, 1007, 1140, 1146, 1520, 1691, 1844, 2834
GlaI GCGC 2 cut(s) 266, 2621
GsaI CCCAGC 1 cut(s) 1727
GsuI CTGGAG 3 cut(s) 359, 673, 2490
HapII CCGG 3 cut(s) 677, 996, 1228
HgaI GACGC 2 cut(s) 1233, 1828
HhaI GCGC 2 cut(s) 267, 2622
Hin1I GRCGYC 3 cut(s) 334, 1225, 1839
Hin6I GCGC 2 cut(s) 265, 2620
HinP1I GCGC 2 cut(s) 265, 2620
HincII GTYRAC 3 cut(s) 657, 1849, 2304
HindII GTYRAC 3 cut(s) 657, 1849, 2304
HindIII AAGCTT 3 cut(s) 1889, 2263, 2870
HpaI GTTAAC 2 cut(s) 657, 2304
HpaII CCGG 3 cut(s) 677, 996, 1228
HphI GGTGA 5 cut(s) 422, 601, 666, 710, 1147
Hpy99I CGWCG 1 cut(s) 336
HpyAV CCTTC 9 cut(s) 386, 394, 776, 1313, 1480, 1816, 1858, 2326, 2507
HpyCH4III ACNGT 8 cut(s) 26, 698, 1176, 1306, 1600, 2153, 2195, 2329
HpyCH4IV ACGT 4 cut(s) 197, 334, 2079, 2225
HpySE526I ACGT 4 cut(s) 197, 334, 2079, 2225
Hsp92I GRCGYC 3 cut(s) 334, 1225, 1839
HspAI GCGC 2 cut(s) 265, 2620
KspAI GTTAAC 2 cut(s) 657, 2304
LguI GCTCTTC 2 cut(s) 99, 1155
LmnI GCTCC 6 cut(s) 346, 741, 749, 1105, 1516, 2591
MaeI CTAG 9 cut(s) 498, 875, 1007, 1140, 1146, 1520, 1691, 1844, 2834
MaeII ACGT 4 cut(s) 197, 334, 2079, 2225
MaeIII GTNAC 7 cut(s) 410, 804, 1135, 1300, 1471, 1544, 2063
MflI RGATCY 2 cut(s) 1330, 2846
MhlI GDGCHC 5 cut(s) 619, 844, 874, 1363, 1605
MlsI TGGCCA 4 cut(s) 623, 1963, 2277, 2504
MluCI AATT 9 cut(s) 54, 125, 418, 444, 827, 2043, 2235, 2366, 2607
MluNI TGGCCA 4 cut(s) 623, 1963, 2277, 2504
MlyI GAGTC 6 cut(s) 188, 645, 680, 812, 2060, 2593
MmeI TCCRAC 2 cut(s) 1179, 1896
Mox20I TGGCCA 4 cut(s) 623, 1963, 2277, 2504
Mph1103I ATGCAT 1 cut(s) 531
MroXI GAANNNNTTC 1 cut(s) 568
MscI TGGCCA 4 cut(s) 623, 1963, 2277, 2504
MseI TTAA 9 cut(s) 20, 57, 204, 518, 656, 2303, 2340, 2684, 2840
Msp20I TGGCCA 4 cut(s) 623, 1963, 2277, 2504
MspA1I CMGCKG 3 cut(s) 738, 1766, 2429
MspI CCGG 3 cut(s) 677, 996, 1228
MspR9I CCNGG 9 cut(s) 44, 997, 1229, 1281, 1529, 2157, 2279, 2469, 2589
Mva1269I GAATGC 4 cut(s) 1000, 1505, 1925, 2028
MvaI CCWGG 7 cut(s) 44, 1281, 1529, 2157, 2279, 2469, 2589
MvnI CGCG 1 cut(s) 783
NciI CCSGG 2 cut(s) 997, 1229
NcoI CCATGG 2 cut(s) 2411, 2487
NheI GCTAGC 1 cut(s) 1519
NlaIV GGNNCC 9 cut(s) 751, 778, 970, 1737, 2311, 2348, 2493, 2593, 2848
NmeAIII GCCGAG 2 cut(s) 825, 2581
NmuCI GTSAC 5 cut(s) 410, 804, 1135, 1471, 2063
NsiI ATGCAT 1 cut(s) 531
NspI RCATGY 1 cut(s) 1258
PaeR7I CTCGAG 1 cut(s) 32
PceI AGGCCT 1 cut(s) 1490
PciSI GCTCTTC 2 cut(s) 99, 1155
PcsI WCGNNNNNNNCGW 1 cut(s) 30
PctI GAATGC 4 cut(s) 1000, 1505, 1925, 2028
PdmI GAANNNNTTC 1 cut(s) 568
PfeI GAWTC 4 cut(s) 453, 569, 1375, 2100
PflFI GACNNNGTC 1 cut(s) 333
PflMI CCANNNNNTGG 1 cut(s) 731
PfoI TCCNGGA 1 cut(s) 42
PleI GAGTC 6 cut(s) 187, 644, 680, 811, 2060, 2593
PpsI GAGTC 6 cut(s) 187, 644, 680, 811, 2060, 2593
PpuMI RGGWCCY 2 cut(s) 373, 1805
PsiI TTATAA 2 cut(s) 2240, 2577
Psp124BI GAGCTC 2 cut(s) 874, 1363
Psp5II RGGWCCY 2 cut(s) 373, 1805
Psp6I CCWGG 7 cut(s) 42, 1279, 1527, 2155, 2277, 2467, 2587
PspFI CCCAGC 1 cut(s) 1723
PspGI CCWGG 7 cut(s) 42, 1279, 1527, 2155, 2277, 2467, 2587
PspN4I GGNNCC 9 cut(s) 751, 778, 970, 1737, 2311, 2348, 2493, 2593, 2848
PspPI GGNCC 4 cut(s) 373, 776, 1805, 2484
PspPPI RGGWCCY 2 cut(s) 373, 1805
PsrI GAACNNNNNNTAC 2 cut(s) 1169, 1201
PstNI CAGNNNCTG 1 cut(s) 2173
PsuI RGATCY 2 cut(s) 1330, 2846
PsyI GACNNNGTC 1 cut(s) 333
PvuII CAGCTG 2 cut(s) 1766, 2429
RsaI GTAC 8 cut(s) 146, 791, 1144, 2040, 2218, 2459, 2715, 2749
RsaNI GTAC 8 cut(s) 145, 790, 1143, 2039, 2217, 2458, 2714, 2748
SacI GAGCTC 2 cut(s) 874, 1363
SapI GCTCTTC 2 cut(s) 99, 1155
SaqAI TTAA 9 cut(s) 20, 57, 204, 518, 656, 2303, 2340, 2684, 2840
Sau96I GGNCC 4 cut(s) 373, 776, 1805, 2484
ScaI AGTACT 2 cut(s) 146, 1144
SchI GAGTC 6 cut(s) 188, 645, 680, 812, 2060, 2593
ScrFI CCNGG 9 cut(s) 44, 997, 1229, 1281, 1529, 2157, 2279, 2469, 2589
SduI GDGCHC 5 cut(s) 619, 844, 874, 1363, 1605
SfcI CTRYAG 2 cut(s) 640, 2106
Sfr274I CTCGAG 1 cut(s) 32
SinI GGWCC 4 cut(s) 373, 776, 1805, 2484
SlaI CTCGAG 1 cut(s) 32
SmlI CTYRAG 5 cut(s) 32, 248, 1193, 1604, 2560
SmoI CTYRAG 5 cut(s) 32, 248, 1193, 1604, 2560
SpeI ACTAGT 2 cut(s) 1139, 2833
Sse9I AATT 9 cut(s) 54, 125, 418, 444, 827, 2043, 2235, 2366, 2607
SseBI AGGCCT 1 cut(s) 1490
SspMI CTAG 9 cut(s) 498, 875, 1007, 1140, 1146, 1520, 1691, 1844, 2834
SstI GAGCTC 2 cut(s) 874, 1363
StuI AGGCCT 1 cut(s) 1490
StyD4I CCNGG 9 cut(s) 42, 995, 1227, 1279, 1527, 2155, 2277, 2467, 2587
StyI CCWWGG 5 cut(s) 1006, 1243, 2411, 2487, 2495
TaaI ACNGT 8 cut(s) 26, 698, 1176, 1306, 1600, 2153, 2195, 2329
TaiI ACGT 4 cut(s) 200, 337, 2082, 2228
TaqI TCGA 7 cut(s) 33, 1052, 1095, 1188, 1614, 2454, 2708
TaqII GACCGA 2 cut(s) 1733, 2725
TasI AATT 9 cut(s) 54, 125, 418, 444, 827, 2043, 2235, 2366, 2607
TatI WGTACW 5 cut(s) 144, 789, 1142, 2457, 2713
TauI GCSGC 1 cut(s) 357
TfiI GAWTC 4 cut(s) 453, 569, 1375, 2100
Tru1I TTAA 9 cut(s) 20, 57, 204, 518, 656, 2303, 2340, 2684, 2840
Tru9I TTAA 9 cut(s) 20, 57, 204, 518, 656, 2303, 2340, 2684, 2840
TscAI CASTG 8 cut(s) 1172, 1539, 1788, 1887, 1976, 2401, 2727, 2869
TseFI GTSAC 5 cut(s) 410, 804, 1135, 1471, 2063
Tsp45I GTSAC 5 cut(s) 410, 804, 1135, 1471, 2063
TspDTI ATGAA 3 cut(s) 1100, 2817, 2867
TspGWI ACGGA 4 cut(s) 1815, 2127, 2552, 2839
TspRI CASTG 8 cut(s) 1172, 1539, 1788, 1887, 1976, 2401, 2727, 2869
Tth111I GACNNNGTC 1 cut(s) 333
Van91I CCANNNNNTGG 1 cut(s) 731
VpaK11BI GGWCC 4 cut(s) 373, 776, 1805, 2484
XapI RAATTY 2 cut(s) 827, 2366
XceI RCATGY 1 cut(s) 1258
XcmI CCANNNNNNNNNTGG 1 cut(s) 2210
XhoI CTCGAG 1 cut(s) 32
XmaJI CCTAGG 1 cut(s) 1006
XmiI GTMKAC 2 cut(s) 684, 2109
XmnI GAANNNNTTC 1 cut(s) 568
XspI CTAG 9 cut(s) 498, 875, 1007, 1140, 1146, 1520, 1691, 1844, 2834
ZraI GACGTC 1 cut(s) 335
ZrmI AGTACT 2 cut(s) 146, 1144
Zsp2I ATGCAT 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.