FvH4_2g02960

Domain of unknown function (DUF4598)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010025
Physical Location & Seq
Reverse (-)
2297994 .. 2301150
3157 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g02960.t2

Sequence Viewer

Length: 552 bp
ATGGGAACTACAAGCAAAGACCTTCTGCGTTTGGAGCATAACCACCCCTCAATTTCACCTCTAGAATCAACTCTACTTGTTTGTAAAAATGGATCCCAGGCTACCCAAACAAAGAAGCCCATCATTGCTCCTCCTCCCAAAAGCCAGTTGTTGGGAAAAATGAGGAACTTCTTGGGAGTCATATCAGAAGCTAATGAAAAATTGCAGCTTGATGCAAAGGATAATCCAGACAAATATGACATAGAAGCGCTCACCGGAGATGAGTCTCAAGTTGTTAGTATGGAATGCATGCTTGGCTTTGTTGATCTTCAAACTCCTGAGGCTGTGGCTGCAGCAGAAGCTGCAGTTTCTGGTTATCAACCTCTGATACCTGTGGCAGATAGCAGTAGTGAAGATGAATCAGATGATAGTAGCGCTGATGATGAAGAAGAAAGTAGTGATGACAATGAAGATGATGAAAGCGATGAACAGGGTAAGAAAACTTCCTCCCCAGACAAAAATAGTTCTGTCAAACGGCCCAAAAAGCGGCCTAAAATTGTTGAGCTTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4598

Protein Analysis

184

Amino Acids

19.79

Weight (kDa)

4.42

Isoelectric Point (pI)

58.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NOPCHAP1 PF15370 50 - 179 2.6e-09 NOP protein chaperone 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 151
AciI CCGC 1 cut(s) 526
AclWI GGATC 2 cut(s) 87, 100
AfeI AGCGCT 2 cut(s) 249, 415
AfiI CCNNNNNNNGG 2 cut(s) 151, 525
AgsI TTSAA 1 cut(s) 311
AjnI CCWGG 1 cut(s) 96
AjuI GAANNNNNNNTTGG 2 cut(s) 276, 308
AluBI AGCT 4 cut(s) 191, 208, 341, 544
AluI AGCT 4 cut(s) 191, 208, 341, 544
Alw26I GTCTC 1 cut(s) 270
AlwI GGATC 2 cut(s) 87, 100
AlwNI CAGNNNCTG 2 cut(s) 341, 350
Aor51HI AGCGCT 2 cut(s) 249, 415
AoxI GGCC 2 cut(s) 515, 527
ApeKI GCWGC 4 cut(s) 205, 329, 332, 341
AspLEI GCGC 2 cut(s) 250, 416
AspS9I GGNCC 1 cut(s) 516
AsuHPI GGTGA 2 cut(s) 48, 244
AxyI CCTNAGG 1 cut(s) 318
BamHI GGATCC 1 cut(s) 92
BarI GAAGNNNNNNTAC 2 cut(s) 466, 498
BbvI GCAGC 4 cut(s) 217, 316, 328, 344
BccI CCATC 1 cut(s) 128
BceAI ACGGC 1 cut(s) 530
BciT130I CCWGG 1 cut(s) 98
BcoDI GTCTC 1 cut(s) 270
BfaI CTAG 1 cut(s) 62
BfmI CTRYAG 2 cut(s) 330, 342
BfoI RGCGCY 2 cut(s) 251, 417
BisI GCNGC 5 cut(s) 206, 330, 333, 342, 527
BlsI GCNGC 5 cut(s) 207, 331, 334, 343, 528
Bme1390I CCNGG 1 cut(s) 98
BmgT120I GGNCC 1 cut(s) 516
BmiI GGNNCC 1 cut(s) 94
BmrFI CCNGG 1 cut(s) 98
BmsI GCATC 1 cut(s) 202
BpuEI CTTGAG 1 cut(s) 252
BsaJI CCNNGG 1 cut(s) 96
BsaWI WCCGGW 1 cut(s) 254
Bsc4I CCNNNNNNNGG 2 cut(s) 151, 525
Bse1I ACTGG 1 cut(s) 145
Bse21I CCTNAGG 1 cut(s) 318
Bse3DI GCAATG 1 cut(s) 123
BseBI CCWGG 1 cut(s) 98
BseDI CCNNGG 1 cut(s) 96
BseLI CCNNNNNNNGG 2 cut(s) 151, 525
BseMI GCAATG 1 cut(s) 123
BseMII CTCAG 1 cut(s) 309
BseNI ACTGG 1 cut(s) 145
BseRI GAGGAG 2 cut(s) 120, 123
BseXI GCAGC 4 cut(s) 217, 316, 328, 344
BshFI GGCC 2 cut(s) 517, 529
BsiSI CCGG 1 cut(s) 255
BslI CCNNNNNNNGG 2 cut(s) 151, 525
BsmAI GTCTC 1 cut(s) 270
BsmI GAATGC 1 cut(s) 290
BsnI GGCC 2 cut(s) 517, 529
Bsp143I GATC 2 cut(s) 92, 304
BspACI CCGC 1 cut(s) 526
BspANI GGCC 2 cut(s) 517, 529
BspCNI CTCAG 1 cut(s) 310
BspLI GGNNCC 1 cut(s) 94
BspMAI CTGCAG 2 cut(s) 334, 346
BspPI GGATC 2 cut(s) 87, 100
BsrDI GCAATG 1 cut(s) 123
BsrI ACTGG 1 cut(s) 145
BssECI CCNNGG 1 cut(s) 96
BssMI GATC 2 cut(s) 92, 304
Bst2UI CCWGG 1 cut(s) 98
BstAPI GCANNNNNTGC 1 cut(s) 341
BstC8I GCNNGC 1 cut(s) 290
BstDEI CTNAG 1 cut(s) 318
BstH2I RGCGCY 2 cut(s) 251, 417
BstHHI GCGC 2 cut(s) 250, 416
BstKTI GATC 2 cut(s) 95, 307
BstMAI GTCTC 1 cut(s) 270
BstMBI GATC 2 cut(s) 92, 304
BstMWI GCNNNNNNNGC 6 cut(s) 34, 294, 329, 338, 341, 523
BstNI CCWGG 1 cut(s) 98
BstNSI RCATGY 1 cut(s) 292
BstSCI CCNGG 1 cut(s) 96
BstSFI CTRYAG 2 cut(s) 330, 342
BstV1I GCAGC 4 cut(s) 217, 316, 328, 344
BstX2I RGATCY 1 cut(s) 92
BstYI RGATCY 1 cut(s) 92
Bsu36I CCTNAGG 1 cut(s) 318
BsuRI GGCC 2 cut(s) 517, 529
BtgZI GCGATG 1 cut(s) 477
Cac8I GCNNGC 1 cut(s) 290
CaiI CAGNNNCTG 2 cut(s) 341, 350
CfoI GCGC 2 cut(s) 250, 416
Cfr13I GGNCC 1 cut(s) 516
CviAII CATG 1 cut(s) 289
DdeI CTNAG 1 cut(s) 318
DpnI GATC 2 cut(s) 94, 306
DpnII GATC 2 cut(s) 92, 304
Eco47III AGCGCT 2 cut(s) 249, 415
Eco81I CCTNAGG 1 cut(s) 318
EcoRII CCWGG 1 cut(s) 96
EcoT22I ATGCAT 1 cut(s) 290
FaeI CATG 1 cut(s) 292
FaiI YATR 7 cut(s) 39, 182, 237, 242, 281, 290, 550
FatI CATG 1 cut(s) 288
Fnu4HI GCNGC 5 cut(s) 206, 330, 333, 342, 527
Fsp4HI GCNGC 5 cut(s) 206, 330, 333, 342, 527
FspBI CTAG 1 cut(s) 62
GlaI GCGC 2 cut(s) 249, 415
GluI GCNGC 5 cut(s) 206, 330, 333, 342, 527
HaeII RGCGCY 2 cut(s) 251, 417
HaeIII GGCC 2 cut(s) 517, 529
HapII CCGG 1 cut(s) 255
HhaI GCGC 2 cut(s) 250, 416
Hin1II CATG 1 cut(s) 292
Hin6I GCGC 2 cut(s) 248, 414
HinP1I GCGC 2 cut(s) 248, 414
HinfI GANTC 4 cut(s) 65, 177, 263, 398
HpaII CCGG 1 cut(s) 255
HphI GGTGA 2 cut(s) 48, 244
Hpy188I TCNGA 3 cut(s) 187, 366, 403
Hpy188III TCNNGA 3 cut(s) 62, 227, 317
HpyAV CCTTC 1 cut(s) 32
HpyCH4V TGCA 5 cut(s) 205, 215, 288, 332, 344
HpyF10VI GCNNNNNNNGC 6 cut(s) 34, 294, 329, 338, 341, 523
HpyF3I CTNAG 1 cut(s) 318
Hsp92II CATG 1 cut(s) 292
HspAI GCGC 2 cut(s) 248, 414
Kzo9I GATC 2 cut(s) 92, 304
LmnI GCTCC 2 cut(s) 34, 133
Lsp1109I GCAGC 4 cut(s) 217, 316, 328, 344
LweI GCATC 1 cut(s) 202
MaeI CTAG 1 cut(s) 62
MalI GATC 2 cut(s) 94, 306
MboI GATC 2 cut(s) 92, 304
MboII GAAGA 5 cut(s) 299, 404, 437, 440, 461
MflI RGATCY 1 cut(s) 92
MluCI AATT 3 cut(s) 51, 200, 534
MlyI GAGTC 2 cut(s) 186, 272
MnlI CCTC 8 cut(s) 58, 69, 141, 144, 156, 313, 372, 496
Mph1103I ATGCAT 1 cut(s) 290
MspI CCGG 1 cut(s) 255
MspR9I CCNGG 1 cut(s) 98
Mva1269I GAATGC 1 cut(s) 290
MvaI CCWGG 1 cut(s) 98
MwoI GCNNNNNNNGC 6 cut(s) 34, 294, 329, 338, 341, 523
NdeII GATC 2 cut(s) 92, 304
NlaIII CATG 1 cut(s) 292
NlaIV GGNNCC 1 cut(s) 94
NsiI ATGCAT 1 cut(s) 290
NspI RCATGY 1 cut(s) 292
PaeI GCATGC 1 cut(s) 292
PctI GAATGC 1 cut(s) 290
PfeI GAWTC 2 cut(s) 65, 398
PflMI CCANNNNNTGG 1 cut(s) 151
PkrI GCNGC 5 cut(s) 207, 331, 334, 343, 528
PleI GAGTC 2 cut(s) 185, 271
PpsI GAGTC 2 cut(s) 185, 271
Psp6I CCWGG 1 cut(s) 96
PspGI CCWGG 1 cut(s) 96
PspN4I GGNNCC 1 cut(s) 94
PspPI GGNCC 1 cut(s) 516
PstI CTGCAG 2 cut(s) 334, 346
PstNI CAGNNNCTG 2 cut(s) 341, 350
PsuI RGATCY 1 cut(s) 92
SatI GCNGC 5 cut(s) 206, 330, 333, 342, 527
Sau3AI GATC 2 cut(s) 92, 304
Sau96I GGNCC 1 cut(s) 516
SchI GAGTC 2 cut(s) 186, 272
ScrFI CCNGG 1 cut(s) 98
SetI ASST 8 cut(s) 24, 61, 193, 210, 343, 364, 373, 546
SfaNI GCATC 1 cut(s) 202
SfcI CTRYAG 2 cut(s) 330, 342
SmlI CTYRAG 1 cut(s) 267
SmoI CTYRAG 1 cut(s) 267
SphI GCATGC 1 cut(s) 292
Sse9I AATT 3 cut(s) 51, 200, 534
SsiI CCGC 1 cut(s) 526
SspMI CTAG 1 cut(s) 62
StyD4I CCNGG 1 cut(s) 96
TasI AATT 3 cut(s) 51, 200, 534
TauI GCSGC 1 cut(s) 529
TfiI GAWTC 2 cut(s) 65, 398
TseI GCWGC 4 cut(s) 205, 329, 332, 341
TspDTI ATGAA 7 cut(s) 210, 411, 438, 462, 471, 480, 537
Van91I CCANNNNNTGG 1 cut(s) 151
XbaI TCTAGA 1 cut(s) 61
XceI RCATGY 1 cut(s) 292
XspI CTAG 1 cut(s) 62
Zsp2I ATGCAT 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.