Rroxscaffold_180G00433270

Domain of unknown function (DUF4598)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000180
OG0010025
Physical Location & Seq
Forward (+)
119845 .. 122009
2165 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_180G00433270.1

Sequence Viewer

Length: 567 bp
ATGGGAACTCCAAGCAAAGATCTTCTGCAATTGGAGCACAACAAGTCCTCAATTTCACCACTAGAATCCACACTACTTGTTTGTAAAAATGAATCCCAGCCTGCCCAAACAAAGAAGCCCATCATTGCTCCTCCTCCCAAAAGCCAGTTGTTGGGAAAAATGAGGAACTTCTTGGGAGTCATGTCAGAAGCTAATGAAAAATTGCAGCTTGATGCGAAGGATAATCCAGAAAAATATGACATCGAAGCGCTCACCGGAGATGAGTCTCAAGTCGTTAGTGTGGAATGTATGTTGGGTGTTGTTGATCTTCAAACTCCTGAGGCTGTGGCTGCAGCAGAAGCTGCTGTTTCTGGTTATCAGCCTGTGATACCTGTGGAGGATAGCAGTAGTGGAGAGGAATCAGATGATAGTAGCAGTGATGATGAAGAAGAAAGTAGCGATGACAATGAAGTTGACGAAAGCAATGAGGATAAGAAAACATCCTCTCCGGACAAAATTAATTCTCTGAGTGAAGAACTTAGTAATAAGCGGCCCAAAAAGCGGCCTAAAATTGTTGAGCTTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4598

Protein Analysis

188

Amino Acids

20.37

Weight (kDa)

4.36

Isoelectric Point (pI)

72.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NOPCHAP1 PF15370 50 - 187 2.3e-12 NOP protein chaperone 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 151
AccIII TCCGGA 1 cut(s) 487
AciI CCGC 2 cut(s) 529, 541
AfeI AGCGCT 1 cut(s) 249
AfiI CCNNNNNNNGG 2 cut(s) 151, 540
AgsI TTSAA 1 cut(s) 311
AluBI AGCT 4 cut(s) 191, 208, 341, 559
AluI AGCT 4 cut(s) 191, 208, 341, 559
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 1 cut(s) 270
AlwNI CAGNNNCTG 1 cut(s) 341
Aor13HI TCCGGA 1 cut(s) 487
Aor51HI AGCGCT 1 cut(s) 249
AoxI GGCC 2 cut(s) 530, 542
ApeKI GCWGC 4 cut(s) 205, 329, 332, 341
AseI ATTAAT 1 cut(s) 498
AspLEI GCGC 1 cut(s) 250
AspS9I GGNCC 1 cut(s) 531
AsuHPI GGTGA 2 cut(s) 48, 244
AxyI CCTNAGG 1 cut(s) 318
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 4 cut(s) 217, 316, 328, 344
BccI CCATC 1 cut(s) 128
BcoDI GTCTC 1 cut(s) 270
BfaI CTAG 1 cut(s) 62
BfmI CTRYAG 1 cut(s) 330
BfoI RGCGCY 1 cut(s) 251
BglII AGATCT 1 cut(s) 19
BisI GCNGC 6 cut(s) 206, 330, 333, 342, 530, 542
BlsI GCNGC 6 cut(s) 207, 331, 334, 343, 531, 543
BmgT120I GGNCC 1 cut(s) 531
BmsI GCATC 1 cut(s) 202
BpuEI CTTGAG 1 cut(s) 252
BsaWI WCCGGW 2 cut(s) 254, 487
BsaXI ACNNNNNCTCC 2 cut(s) 469, 499
Bsc4I CCNNNNNNNGG 2 cut(s) 151, 540
Bse1I ACTGG 1 cut(s) 145
Bse21I CCTNAGG 1 cut(s) 318
Bse3DI GCAATG 2 cut(s) 123, 469
BseAI TCCGGA 1 cut(s) 487
BseGI GGATG 1 cut(s) 479
BseLI CCNNNNNNNGG 2 cut(s) 151, 540
BseMI GCAATG 2 cut(s) 123, 469
BseMII CTCAG 2 cut(s) 309, 497
BseNI ACTGG 1 cut(s) 145
BseRI GAGGAG 2 cut(s) 120, 123
BseXI GCAGC 4 cut(s) 217, 316, 328, 344
BseYI CCCAGC 1 cut(s) 96
BshFI GGCC 2 cut(s) 532, 544
BsiHKAI GWGCWC 1 cut(s) 39
BsiSI CCGG 2 cut(s) 255, 488
BslI CCNNNNNNNGG 2 cut(s) 151, 540
BsmAI GTCTC 1 cut(s) 270
BsnI GGCC 2 cut(s) 532, 544
Bsp1286I GDGCHC 1 cut(s) 39
Bsp13I TCCGGA 1 cut(s) 487
Bsp143I GATC 2 cut(s) 19, 304
BspACI CCGC 2 cut(s) 529, 541
BspANI GGCC 2 cut(s) 532, 544
BspCNI CTCAG 2 cut(s) 310, 498
BspEI TCCGGA 1 cut(s) 487
BspMAI CTGCAG 1 cut(s) 334
BsrDI GCAATG 2 cut(s) 123, 469
BsrI ACTGG 1 cut(s) 145
BssMI GATC 2 cut(s) 19, 304
BstAPI GCANNNNNTGC 1 cut(s) 341
BstC8I GCNNGC 1 cut(s) 102
BstDEI CTNAG 3 cut(s) 318, 506, 518
BstF5I GGATG 1 cut(s) 479
BstH2I RGCGCY 1 cut(s) 251
BstHHI GCGC 1 cut(s) 250
BstKTI GATC 2 cut(s) 22, 307
BstMAI GTCTC 1 cut(s) 270
BstMBI GATC 2 cut(s) 19, 304
BstMWI GCNNNNNNNGC 5 cut(s) 34, 329, 338, 341, 538
BstSFI CTRYAG 1 cut(s) 330
BstV1I GCAGC 4 cut(s) 217, 316, 328, 344
BstX2I RGATCY 1 cut(s) 19
BstYI RGATCY 1 cut(s) 19
Bsu36I CCTNAGG 1 cut(s) 318
BsuRI GGCC 2 cut(s) 532, 544
BtgZI GCGATG 1 cut(s) 453
BtsCI GGATG 1 cut(s) 479
BtsI GCAGTG 1 cut(s) 421
BtsIMutI CAGTG 1 cut(s) 421
Cac8I GCNNGC 1 cut(s) 102
CaiI CAGNNNCTG 1 cut(s) 341
CfoI GCGC 1 cut(s) 250
Cfr13I GGNCC 1 cut(s) 531
CspCI CAANNNNNGTGG 2 cut(s) 58, 93
CviAII CATG 1 cut(s) 181
DdeI CTNAG 3 cut(s) 318, 506, 518
DpnI GATC 2 cut(s) 21, 306
DpnII GATC 2 cut(s) 19, 304
Eco47III AGCGCT 1 cut(s) 249
Eco81I CCTNAGG 1 cut(s) 318
FaeI CATG 1 cut(s) 184
FaiI YATR 4 cut(s) 182, 237, 290, 565
FatI CATG 1 cut(s) 180
Fnu4HI GCNGC 6 cut(s) 206, 330, 333, 342, 530, 542
FokI GGATG 1 cut(s) 466
Fsp4HI GCNGC 6 cut(s) 206, 330, 333, 342, 530, 542
FspBI CTAG 1 cut(s) 62
GlaI GCGC 1 cut(s) 249
GluI GCNGC 6 cut(s) 206, 330, 333, 342, 530, 542
GsaI CCCAGC 1 cut(s) 100
HaeII RGCGCY 1 cut(s) 251
HaeIII GGCC 2 cut(s) 532, 544
HapII CCGG 2 cut(s) 255, 488
HhaI GCGC 1 cut(s) 250
Hin1II CATG 1 cut(s) 184
Hin6I GCGC 1 cut(s) 248
HinP1I GCGC 1 cut(s) 248
HincII GTYRAC 1 cut(s) 454
HindII GTYRAC 1 cut(s) 454
HinfI GANTC 5 cut(s) 65, 92, 177, 263, 398
HpaII CCGG 2 cut(s) 255, 488
HphI GGTGA 2 cut(s) 48, 244
Hpy166II GTNNAC 1 cut(s) 454
Hpy188I TCNGA 3 cut(s) 187, 403, 507
Hpy188III TCNNGA 3 cut(s) 227, 317, 488
Hpy8I GTNNAC 1 cut(s) 454
HpyAV CCTTC 1 cut(s) 211
HpyCH4V TGCA 3 cut(s) 28, 205, 332
HpyF10VI GCNNNNNNNGC 5 cut(s) 34, 329, 338, 341, 538
HpyF3I CTNAG 3 cut(s) 318, 506, 518
Hsp92II CATG 1 cut(s) 184
HspAI GCGC 1 cut(s) 248
Kpn2I TCCGGA 1 cut(s) 487
Kzo9I GATC 2 cut(s) 19, 304
LmnI GCTCC 2 cut(s) 34, 133
Lsp1109I GCAGC 4 cut(s) 217, 316, 328, 344
LweI GCATC 1 cut(s) 202
MaeI CTAG 1 cut(s) 62
MalI GATC 2 cut(s) 21, 306
MboI GATC 2 cut(s) 19, 304
MboII GAAGA 5 cut(s) 14, 299, 437, 440, 524
MfeI CAATTG 1 cut(s) 29
MflI RGATCY 1 cut(s) 19
MhlI GDGCHC 1 cut(s) 39
MluCI AATT 6 cut(s) 29, 51, 200, 495, 499, 549
MlyI GAGTC 2 cut(s) 186, 272
MnlI CCTC 9 cut(s) 58, 141, 144, 156, 313, 370, 388, 460, 493
MroI TCCGGA 1 cut(s) 487
MseI TTAA 1 cut(s) 498
MspI CCGG 2 cut(s) 255, 488
MunI CAATTG 1 cut(s) 29
MwoI GCNNNNNNNGC 5 cut(s) 34, 329, 338, 341, 538
NdeII GATC 2 cut(s) 19, 304
NlaIII CATG 1 cut(s) 184
PfeI GAWTC 3 cut(s) 65, 92, 398
PflMI CCANNNNNTGG 1 cut(s) 151
PkrI GCNGC 6 cut(s) 207, 331, 334, 343, 531, 543
PleI GAGTC 2 cut(s) 185, 271
PpsI GAGTC 2 cut(s) 185, 271
PshBI ATTAAT 1 cut(s) 498
PspFI CCCAGC 1 cut(s) 96
PspPI GGNCC 1 cut(s) 531
PstI CTGCAG 1 cut(s) 334
PstNI CAGNNNCTG 1 cut(s) 341
PsuI RGATCY 1 cut(s) 19
SaqAI TTAA 1 cut(s) 498
SatI GCNGC 6 cut(s) 206, 330, 333, 342, 530, 542
Sau3AI GATC 2 cut(s) 19, 304
Sau96I GGNCC 1 cut(s) 531
SchI GAGTC 2 cut(s) 186, 272
SduI GDGCHC 1 cut(s) 39
SetI ASST 5 cut(s) 193, 210, 343, 373, 561
SfaNI GCATC 1 cut(s) 202
SfcI CTRYAG 1 cut(s) 330
SmlI CTYRAG 1 cut(s) 267
SmoI CTYRAG 1 cut(s) 267
Sse9I AATT 6 cut(s) 29, 51, 200, 495, 499, 549
SsiI CCGC 2 cut(s) 529, 541
SspMI CTAG 1 cut(s) 62
TaqI TCGA 1 cut(s) 243
TasI AATT 6 cut(s) 29, 51, 200, 495, 499, 549
TauI GCSGC 2 cut(s) 532, 544
TfiI GAWTC 3 cut(s) 65, 92, 398
Tru1I TTAA 1 cut(s) 498
Tru9I TTAA 1 cut(s) 498
TscAI CASTG 1 cut(s) 421
TseI GCWGC 4 cut(s) 205, 329, 332, 341
TspDTI ATGAA 5 cut(s) 105, 210, 438, 462, 552
TspRI CASTG 1 cut(s) 421
Van91I CCANNNNNTGG 1 cut(s) 151
VspI ATTAAT 1 cut(s) 498
XspI CTAG 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.