MD10G1112800.v1.1

Domain of unknown function (DUF4598)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010025
Physical Location & Seq
Forward (+)
18633337 .. 18635737
2401 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1112800.v1.1.491

Sequence Viewer

Length: 663 bp
ATGGGAAATACAAGCAAAGACCTTCTGCAATTGGAGCACAAAAAGGCTTCAACTTCACCCTTAGAAAGCACGCTACTTGTTTGTAAAAATGCCTCAACGAAGAAAGCTCAACCCGAAGGGAAACTCATCCCTGCTCTTCCTCTTCCCAAAAGCCAAGTTTTGGGACAAGTAAGGGACTTTTTGGGAGTCATATCAGAAGCTAACGAACGACTATACCGGGATGCAAAGGATAATCCAGAAAACTATGATATTGAAGTGCTCACGGGCAATGAATCTGAAGTCATTAATATGGAGTTGATGCTGGGCGTTGCTGATCTTCAAACTCCTGAGGCTGTGACTGCTGCTGAATCTGCAATTTCTGGTTGTCAGCCTGTTATACCATTAGCCGTCAACAGTAGCGGAGAGGAATCAGAGGATAGCAGTGATGATGACAGTAGCAGCAAGGATGGTGATGACGAAGATAGTTCCGACGACAGTAGCAGCGAAGATAGTTTCGATGAAGATAGTGATGATATCAAAGATGATACAAGTGATAAAGATGATAAGGAAACATGCTTTCATGCGAAACTCAAAACACGCGAGCCTGATCAAGATAATGCTTTGAGTGAAGATGTTGGTAACAATCGGTCCAAAAAACGACCAAGAATTGTTGAGCTCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4598

Protein Analysis

221

Amino Acids

23.89

Weight (kDa)

4.24

Isoelectric Point (pI)

48.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NOPCHAP1 PF15370 54 - 158 6.5e-12 NOP protein chaperone 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 160
AccII CGCG 1 cut(s) 579
AciI CCGC 1 cut(s) 399
AcuI CTGAAG 1 cut(s) 297
AfiI CCNNNNNNNGG 1 cut(s) 160
AgsI TTSAA 3 cut(s) 51, 254, 320
AluBI AGCT 3 cut(s) 107, 200, 655
AluI AGCT 3 cut(s) 107, 200, 655
Alw21I GWGCWC 3 cut(s) 39, 261, 657
ApeKI GCWGC 3 cut(s) 341, 438, 480
AseI ATTAAT 1 cut(s) 285
AspS9I GGNCC 1 cut(s) 627
AsuC2I CCSGG 1 cut(s) 218
AsuHPI GGTGA 2 cut(s) 48, 461
AvaII GGWCC 1 cut(s) 627
AxyI CCTNAGG 1 cut(s) 327
BanII GRGCYC 1 cut(s) 657
Bbv12I GWGCWC 3 cut(s) 39, 261, 657
BbvI GCAGC 3 cut(s) 328, 450, 492
BccI CCATC 1 cut(s) 440
BceAI ACGGC 1 cut(s) 371
BclI TGATCA 1 cut(s) 586
BcnI CCSGG 1 cut(s) 218
BisI GCNGC 3 cut(s) 342, 439, 481
BlsI GCNGC 3 cut(s) 343, 440, 482
Bme1390I CCNGG 1 cut(s) 218
Bme18I GGWCC 1 cut(s) 627
BmgT120I GGNCC 1 cut(s) 627
BmrFI CCNGG 1 cut(s) 218
BmsI GCATC 2 cut(s) 211, 288
BpuMI CCSGG 1 cut(s) 218
Bsc4I CCNNNNNNNGG 1 cut(s) 160
Bse21I CCTNAGG 1 cut(s) 327
Bse3DI GCAATG 1 cut(s) 274
BseGI GGATG 3 cut(s) 126, 226, 451
BseLI CCNNNNNNNGG 1 cut(s) 160
BseMI GCAATG 1 cut(s) 274
BseMII CTCAG 1 cut(s) 318
BseXI GCAGC 3 cut(s) 328, 450, 492
BseYI CCCAGC 1 cut(s) 301
Bsh1236I CGCG 1 cut(s) 579
BsiHKAI GWGCWC 3 cut(s) 39, 261, 657
BsiSI CCGG 1 cut(s) 217
BslFI GGGAC 2 cut(s) 177, 188
BslI CCNNNNNNNGG 1 cut(s) 160
BsmFI GGGAC 2 cut(s) 177, 188
Bsp1286I GDGCHC 3 cut(s) 39, 261, 657
Bsp143I GATC 2 cut(s) 313, 586
BspACI CCGC 1 cut(s) 399
BspCNI CTCAG 1 cut(s) 319
BspFNI CGCG 1 cut(s) 579
BspHI TCATGA 1 cut(s) 659
BspQI GCTCTTC 1 cut(s) 141
BsrDI GCAATG 1 cut(s) 274
BssMI GATC 2 cut(s) 313, 586
Bst4CI ACNGT 3 cut(s) 395, 434, 476
Bst6I CTCTTC 2 cut(s) 141, 147
BstC8I GCNNGC 2 cut(s) 71, 581
BstDEI CTNAG 2 cut(s) 61, 327
BstF5I GGATG 3 cut(s) 126, 226, 451
BstFNI CGCG 1 cut(s) 579
BstKTI GATC 2 cut(s) 316, 589
BstMBI GATC 2 cut(s) 313, 586
BstMWI GCNNNNNNNGC 3 cut(s) 34, 338, 350
BstNSI RCATGY 1 cut(s) 555
BstSCI CCNGG 1 cut(s) 216
BstUI CGCG 1 cut(s) 579
BstV1I GCAGC 3 cut(s) 328, 450, 492
Bsu36I CCTNAGG 1 cut(s) 327
BtsCI GGATG 3 cut(s) 126, 226, 451
BtsI GCAGTG 1 cut(s) 427
BtsIMutI CAGTG 1 cut(s) 427
Cac8I GCNNGC 2 cut(s) 71, 581
CciI TCATGA 1 cut(s) 659
Cfr13I GGNCC 1 cut(s) 627
CviAII CATG 3 cut(s) 552, 560, 660
CviJI RGCY 9 cut(s) 47, 107, 153, 200, 332, 370, 386, 583, 655
CviKI_1 RGCY 9 cut(s) 47, 107, 153, 200, 332, 370, 386, 583, 655
DdeI CTNAG 2 cut(s) 61, 327
DpnI GATC 2 cut(s) 315, 588
DpnII GATC 2 cut(s) 313, 586
Eam1104I CTCTTC 2 cut(s) 141, 147
EarI CTCTTC 2 cut(s) 141, 147
Ecl136II GAGCTC 1 cut(s) 655
Eco24I GRGCYC 1 cut(s) 657
Eco32I GATATC 1 cut(s) 514
Eco47I GGWCC 1 cut(s) 627
Eco53kI GAGCTC 1 cut(s) 655
Eco57I CTGAAG 1 cut(s) 297
Eco81I CCTNAGG 1 cut(s) 327
EcoICRI GAGCTC 1 cut(s) 655
EcoRV GATATC 1 cut(s) 514
EcoT38I GRGCYC 1 cut(s) 657
FaeI CATG 3 cut(s) 555, 563, 663
FaiI YATR 8 cut(s) 191, 214, 246, 290, 377, 553, 561, 661
FaqI GGGAC 2 cut(s) 177, 188
FatI CATG 3 cut(s) 551, 559, 659
FbaI TGATCA 1 cut(s) 586
Fnu4HI GCNGC 3 cut(s) 342, 439, 481
FokI GGATG 3 cut(s) 113, 233, 458
FriOI GRGCYC 1 cut(s) 657
Fsp4HI GCNGC 3 cut(s) 342, 439, 481
GluI GCNGC 3 cut(s) 342, 439, 481
GsaI CCCAGC 1 cut(s) 305
HapII CCGG 1 cut(s) 217
Hin1II CATG 3 cut(s) 555, 563, 663
HincII GTYRAC 1 cut(s) 391
HindII GTYRAC 1 cut(s) 391
HinfI GANTC 4 cut(s) 186, 272, 347, 407
HpaII CCGG 1 cut(s) 217
HphI GGTGA 2 cut(s) 48, 461
Hpy166II GTNNAC 1 cut(s) 391
Hpy188I TCNGA 4 cut(s) 196, 277, 412, 469
Hpy188III TCNNGA 4 cut(s) 236, 326, 590, 660
Hpy8I GTNNAC 1 cut(s) 391
Hpy99I CGWCG 1 cut(s) 473
HpyAV CCTTC 2 cut(s) 32, 110
HpyCH4III ACNGT 3 cut(s) 395, 434, 476
HpyCH4V TGCA 3 cut(s) 28, 224, 353
HpyF10VI GCNNNNNNNGC 3 cut(s) 34, 338, 350
HpyF3I CTNAG 2 cut(s) 61, 327
Hsp92II CATG 3 cut(s) 555, 563, 663
Ksp22I TGATCA 1 cut(s) 586
Kzo9I GATC 2 cut(s) 313, 586
LguI GCTCTTC 1 cut(s) 141
LmnI GCTCC 1 cut(s) 34
LpnPI CCDG 8 cut(s) 144, 230, 249, 287, 339, 345, 384, 597
Lsp1109I GCAGC 3 cut(s) 328, 450, 492
LweI GCATC 2 cut(s) 211, 288
MaeIII GTNAC 2 cut(s) 334, 617
MalI GATC 2 cut(s) 315, 588
MboI GATC 2 cut(s) 313, 586
MboII GAAGA 8 cut(s) 112, 128, 134, 308, 470, 497, 512, 620
MfeI CAATTG 1 cut(s) 29
MhlI GDGCHC 3 cut(s) 39, 261, 657
MluCI AATT 3 cut(s) 29, 354, 645
MlyI GAGTC 1 cut(s) 195
MmeI TCCRAC 1 cut(s) 492
MnlI CCTC 5 cut(s) 103, 150, 322, 397, 406
MseI TTAA 1 cut(s) 285
MslI CAYNNNNRTG 1 cut(s) 287
MspI CCGG 1 cut(s) 217
MspR9I CCNGG 1 cut(s) 218
MunI CAATTG 1 cut(s) 29
MvnI CGCG 1 cut(s) 579
MwoI GCNNNNNNNGC 3 cut(s) 34, 338, 350
NciI CCSGG 1 cut(s) 218
NdeII GATC 2 cut(s) 313, 586
NlaIII CATG 3 cut(s) 555, 563, 663
NmuCI GTSAC 1 cut(s) 334
NspI RCATGY 1 cut(s) 555
PagI TCATGA 1 cut(s) 659
PciSI GCTCTTC 1 cut(s) 141
PfeI GAWTC 3 cut(s) 272, 347, 407
PflMI CCANNNNNTGG 1 cut(s) 160
PkrI GCNGC 3 cut(s) 343, 440, 482
PleI GAGTC 1 cut(s) 194
PpsI GAGTC 1 cut(s) 194
PshBI ATTAAT 1 cut(s) 285
Psp124BI GAGCTC 1 cut(s) 657
PspFI CCCAGC 1 cut(s) 301
PspPI GGNCC 1 cut(s) 627
RseI CAYNNNNRTG 1 cut(s) 287
SacI GAGCTC 1 cut(s) 657
SapI GCTCTTC 1 cut(s) 141
SaqAI TTAA 1 cut(s) 285
SatI GCNGC 3 cut(s) 342, 439, 481
Sau3AI GATC 2 cut(s) 313, 586
Sau96I GGNCC 1 cut(s) 627
SchI GAGTC 1 cut(s) 195
ScrFI CCNGG 1 cut(s) 218
SduI GDGCHC 3 cut(s) 39, 261, 657
SetI ASST 4 cut(s) 24, 109, 202, 657
SfaNI GCATC 2 cut(s) 211, 288
SinI GGWCC 1 cut(s) 627
SmiMI CAYNNNNRTG 1 cut(s) 287
Sse9I AATT 3 cut(s) 29, 354, 645
SsiI CCGC 1 cut(s) 399
SstI GAGCTC 1 cut(s) 657
StyD4I CCNGG 1 cut(s) 216
TaaI ACNGT 3 cut(s) 395, 434, 476
TaqI TCGA 1 cut(s) 495
TaqII GACCGA 1 cut(s) 615
TasI AATT 3 cut(s) 29, 354, 645
TfiI GAWTC 3 cut(s) 272, 347, 407
Tru1I TTAA 1 cut(s) 285
Tru9I TTAA 1 cut(s) 285
TscAI CASTG 1 cut(s) 427
TseFI GTSAC 1 cut(s) 334
TseI GCWGC 3 cut(s) 341, 438, 480
Tsp45I GTSAC 1 cut(s) 334
TspDTI ATGAA 3 cut(s) 285, 513, 548
TspRI CASTG 1 cut(s) 427
Van91I CCANNNNNTGG 1 cut(s) 160
VpaK11BI GGWCC 1 cut(s) 627
VspI ATTAAT 1 cut(s) 285
XceI RCATGY 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.