Rorug05G0528300

Domain of unknown function (DUF4598)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0010025
Physical Location & Seq
Reverse (-)
71747742 .. 71754295
6554 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0528300.1

Sequence Viewer

Length: 2502 bp
ATGGGTCGCTGCACTTTCTCCTCGGACGATATTGTAATAAAGTCTCCGAATGATAAGAAACTGTACAGACTAATTAAGCTTGAGAATGGCCTCACTGCATTGCTCATTCATGACCCTGAGATATACCCACAAGGGTCCAAGAGCCTCGAACCCAGTGAAGAAGATGAAGCAGCAGCTAAAGGTGGAGATGGTGCCTCAAAGACTAAGAAGGCAGCAGCGGCAATGTGCGTTGGAATGGGCAGCTTCTCTGACCATATGGAAGCACAGGGGCTTGCGCACTTTCTTGAACATATGCTCTTCATGGGGAGTACAAAGTTTCCGGTTGAAAATGAGTATAACAGTTACTTGTCCAAGCATGGGGGCTCATCAAATGCTTACACAAAGGAAGAGCACACTTGCTACCATTTTGACGTGAAACGAGAGTTTCTAAAAGGTGCCTTGAAAAGGCTAGAGGCTGTTAATGATGTTCCTATGCTCAACTTGGCATGGACGCTTCCATGTCTTACTCAACACTATTTGAAGAGCCCAGAATATTATTTATGGCATCTCCTTGCGCATGAGAGCAGGGGAGGTTTGCAGTTCTACCTCAAAACTAGAGGGTGGGCAGTATCTCTAAGTGCTTGGGTGGAGAGCTATTCTGTGGCTTATGTCTTTGGCATGAATATTTACCTCACCGACTCTGGATTGGAGAAGATTTTTGAAATTATTGGGTTCGTGTATCAATACATTAAACTATTGCGTCAAGTGTCACCACAAAAATGGATATTTCAAGAACTCCAGGATATTCGGAACATATGCTTTAGATTTGTAGAGGAGCAGCCCCAGGATGACTATGCTTCAGAACTTGCAGGAAATTTACTACTTTATCCAGCAGAGCATGTTATTTCTGCGCAATGTGGGTATGAGATTTGGGATGAGGAATTGATTGAATATGTTCTTAGTTTCTTCAGACCAGACAACATGAGGATGGATGTGATATCAAAGTTTTCAACTATGTCCAAAGACTTTCAGTGCGAGCCTTGGTTTGGGTCACATTATATTGAGGAAGATGTATCTCCGTTTTTGATGGATTTGTGGAAGGATCCTCCAGAGATTGATGTTTCGTTGCATCTCCGAGAAAAGAATGAGTTTATTCCTTCTGATTTTTCCATTCGTTCTGATGGTCTTGATACTACATATACATCTTCTCCGACATGTATACTTGATGAACCTTTGCTTAAGTTCTGGTACAAGCTTGATCGTACTTTTAAAGTTCCACGGGCAAATATATACTTTCGAATCAGTCTAAAGGGTGGATTTGATGACTTGAAGAGTTCTCTGTTGACTGTACTATATATCGACCTTCTTAGAGATGAGCTGAATGAGATTTTATATCAGGCCAGTTTTGCCCTGCTGGATACTTCTTTGTATGTGAATAATTGCTATCTGGAACTGAAGCTGTGGGGCTTCAATGATAAGCTTCCAGCTGTATTGTCAACAATTCTGACAACAACCAAAAATTTCCTGCCAACTTATGATCGTTTCAAGGTTATTAAAGAAAATATGGAGCGTCTGATAAAGAACACTAATATGAATCCTCTGAGTCATGCTGAATACTTGAAAATGCAAGTTTTGTACCAGAGATTCTACGATGTAGATGACCAGTTGCATGTTTTAAATGGATTGTCTGTTTCTGATGTGAAGTCATTTATTCCAGAGCTTTGGTCCCAGCTTTACATTGAAGGCCTTGGTCATGGAAATCTGTTAGAAGAAGAAGCAATTAGCCTTTTGAATATGGTGAAAACAATTTTTTCTGGACAACCACTTCCTACCGAACTGATGCTTAAGAACAAATGTATTTGTCTCGCTCCTGATGCTAACCTCATTAGAGATCTATATTTTCAAATTGAGCGGGCAGTGGGGATTGACTCCACCAGATTGAGAGCATTAATAGATCTTTTTGATGAAATTATAGTCGAACCACTTTACGATCAACTAAGGACGAAGGAGCAGCTGGGATATGTTGTTCAGTGTGACTGGGAGGTGATATTTGGTGTTTTTGGCTTTTATTTCATAGTTCAGTCGTTGGAGTACAACCCAATCTACTTGCAGCGGAGAGTTGACAACTTTATCAATGGTCTGGAAGACATTTTGCAAGGACTGGATGATGTTTCCTTTGAGAATTACAGAGGTGGGCTAATGGCAAAGCTATTGGTGAAAAATTCATCCCTTATACATGAAACGATTAGATTTTGGAATCAGATTATTCATAAAAGGTATACATTTGACTATGCAAAGAGGGTGGCAGAAGAGCTCAGCAGTCTACAGAAGGACGATGTTGTCAACTTTTACAAGACCTACTTGCAACAATCATCTCCCAAGCGTCGGAGACTTGGCATTCGTGTTTGGGGTTGCAACACTGACTTGAAAGAAGCTGAAGAATCGCGACCGGAGTCTGTGCAGGTCATTGAAGACCTTGAAGCCTTTAAGATGTCATCTGAGTTCTATCCTCACGGTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4598

Protein Analysis

833

Amino Acids

96.56

Weight (kDa)

5.13

Isoelectric Point (pI)

46.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 67 - 153 7.7e-25 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 128 - 261 1.6e-08 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 267 - 550 9.7e-77 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 555 - 723 3e-09 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 647 - 745 5.8e-20 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2318
Acc16I TGCGCA 3 cut(s) 276, 555, 891
Acc36I ACCTGC 1 cut(s) 2431
AccB1I GGYRCC 2 cut(s) 191, 434
AccBSI CCGCTC 1 cut(s) 1891
AccI GTMKAC 3 cut(s) 1198, 2258, 2302
AccII CGCG 1 cut(s) 2425
AciI CCGC 3 cut(s) 218, 1891, 2092
AclWI GGATC 2 cut(s) 1076, 1089
AcsI RAATTY 3 cut(s) 853, 1498, 2200
AcuI CTGAAG 4 cut(s) 822, 931, 1454, 2436
AfaI GTAC 7 cut(s) 65, 310, 1229, 1243, 1329, 1616, 2072
AfiI CCNNNNNNNGG 4 cut(s) 357, 444, 1025, 2364
AflII CTTAAG 2 cut(s) 1217, 1823
AflIII ACRYGT 1 cut(s) 1193
AjiI CACGTC 1 cut(s) 412
AjnI CCWGG 2 cut(s) 777, 822
AloI GAACNNNNNNTCC 2 cut(s) 1989, 2021
Alw21I GWGCWC 2 cut(s) 393, 2295
Alw26I GTCTC 3 cut(s) 48, 1847, 2362
AlwI GGATC 2 cut(s) 1076, 1089
AoxI GGCC 3 cut(s) 88, 1377, 1723
ApeKI GCWGC 9 cut(s) 9, 170, 173, 212, 215, 240, 817, 1990, 2089
ApoI RAATTY 3 cut(s) 853, 1498, 2200
ArsI GACNNNNNNTTYG 2 cut(s) 2361, 2393
AseI ATTAAT 1 cut(s) 1928
Asp700I GAANNNNTTC 1 cut(s) 933
AspLEI GCGC 3 cut(s) 277, 556, 892
AspS9I GGNCC 2 cut(s) 135, 1704
AsuHPI GGTGA 5 cut(s) 664, 741, 1789, 2035, 2206
AsuII TTCGAA 1 cut(s) 1276
AvaII GGWCC 2 cut(s) 135, 1704
BamHI GGATCC 1 cut(s) 1081
BanI GGYRCC 2 cut(s) 191, 434
BanII GRGCYC 3 cut(s) 365, 527, 2295
BbsI GAAGAC 2 cut(s) 2130, 2457
Bbv12I GWGCWC 2 cut(s) 393, 2295
BbvI GCAGC 8 cut(s) 182, 185, 224, 227, 252, 829, 2002, 2101
BccI CCATC 4 cut(s) 182, 961, 1060, 1154
BciT130I CCWGG 2 cut(s) 779, 824
BciVI GTATCC 1 cut(s) 1390
BcoDI GTCTC 3 cut(s) 48, 1847, 2362
BfaI CTAG 2 cut(s) 449, 594
BfmI CTRYAG 1 cut(s) 2303
BfrI CTTAAG 2 cut(s) 1217, 1823
BfuAI ACCTGC 1 cut(s) 2431
BfuI GTATCC 1 cut(s) 1390
BglII AGATCT 2 cut(s) 1870, 1933
BlpI GCTNAGC 1 cut(s) 2294
Bme1390I CCNGG 2 cut(s) 779, 824
Bme18I GGWCC 2 cut(s) 135, 1704
BmgBI CACGTC 1 cut(s) 412
BmgT120I GGNCC 2 cut(s) 135, 1704
BmiI GGNNCC 5 cut(s) 136, 193, 436, 1083, 1706
BmrFI CCNGG 2 cut(s) 779, 824
BmrI ACTGGG 2 cut(s) 147, 2026
BmsI GCATC 4 cut(s) 553, 1117, 1809, 1843
BmuI ACTGGG 2 cut(s) 147, 2026
BoxI GACNNNNGTC 1 cut(s) 2431
BpiI GAAGAC 2 cut(s) 2130, 2457
BpmI CTGGAG 2 cut(s) 761, 1071
Bpu1102I GCTNAGC 1 cut(s) 2294
Bpu14I TTCGAA 1 cut(s) 1276
BpuEI CTTGAG 1 cut(s) 101
BsaJI CCNNGG 5 cut(s) 21, 822, 1019, 1256, 1726
BsaWI WCCGGW 2 cut(s) 319, 2428
BsaXI ACNNNNNCTCC 2 cut(s) 1171, 1201
Bsc4I CCNNNNNNNGG 4 cut(s) 357, 444, 1025, 2364
Bse1I ACTGG 5 cut(s) 153, 1380, 1642, 2021, 2145
Bse3DI GCAATG 3 cut(s) 98, 228, 899
BseBI CCWGG 2 cut(s) 779, 824
BseDI CCNNGG 5 cut(s) 21, 822, 1019, 1256, 1726
BseGI GGATG 6 cut(s) 832, 919, 972, 976, 2149, 2204
BseLI CCNNNNNNNGG 4 cut(s) 357, 444, 1025, 2364
BseMI GCAATG 3 cut(s) 98, 228, 899
BseMII CTCAG 4 cut(s) 108, 1571, 2308, 2469
BseNI ACTGG 5 cut(s) 153, 1380, 1642, 2021, 2145
BseRI GAGGAG 2 cut(s) 10, 827
BseXI GCAGC 8 cut(s) 182, 185, 224, 227, 252, 829, 2002, 2101
BseYI CCCAGC 2 cut(s) 1707, 1993
BsgI GTGCAG 1 cut(s) 2459
Bsh1236I CGCG 1 cut(s) 2425
Bsh1285I CGRYCG 1 cut(s) 2429
BshFI GGCC 3 cut(s) 90, 1379, 1725
BshNI GGYRCC 2 cut(s) 191, 434
BsiEI CGRYCG 1 cut(s) 2429
BsiHKAI GWGCWC 2 cut(s) 393, 2295
BsiSI CCGG 2 cut(s) 320, 2429
BslFI GGGAC 1 cut(s) 1690
BslI CCNNNNNNNGG 4 cut(s) 357, 444, 1025, 2364
BsmAI GTCTC 3 cut(s) 48, 1847, 2362
BsmFI GGGAC 1 cut(s) 1690
BsmI GAATGC 1 cut(s) 2376
BsnI GGCC 3 cut(s) 90, 1379, 1725
Bsp119I TTCGAA 1 cut(s) 1276
Bsp1286I GDGCHC 4 cut(s) 365, 393, 527, 2295
Bsp1407I TGTACA 1 cut(s) 63
Bsp143I GATC 6 cut(s) 1081, 1237, 1516, 1870, 1933, 1969
Bsp1720I GCTNAGC 1 cut(s) 2294
Bsp68I TCGCGA 1 cut(s) 2425
BspACI CCGC 3 cut(s) 218, 1891, 2092
BspANI GGCC 3 cut(s) 90, 1379, 1725
BspCNI CTCAG 4 cut(s) 109, 1572, 2307, 2470
BspFNI CGCG 1 cut(s) 2425
BspHI TCATGA 1 cut(s) 109
BspLI GGNNCC 5 cut(s) 136, 193, 436, 1083, 1706
BspMI ACCTGC 1 cut(s) 2431
BspPI GGATC 2 cut(s) 1076, 1089
BspQI GCTCTTC 4 cut(s) 302, 381, 515, 2283
BspT104I TTCGAA 1 cut(s) 1276
BspT107I GGYRCC 2 cut(s) 191, 434
BspTI CTTAAG 2 cut(s) 1217, 1823
BsrBI CCGCTC 1 cut(s) 1891
BsrDI GCAATG 3 cut(s) 98, 228, 899
BsrGI TGTACA 1 cut(s) 63
BsrI ACTGG 5 cut(s) 153, 1380, 1642, 2021, 2145
BssECI CCNNGG 5 cut(s) 21, 822, 1019, 1256, 1726
BssMI GATC 6 cut(s) 1081, 1237, 1516, 1870, 1933, 1969
BssNAI GTATAC 2 cut(s) 1199, 2259
BssT1I CCWWGG 2 cut(s) 1019, 1726
Bst1107I GTATAC 2 cut(s) 1199, 2259
Bst2UI CCWGG 2 cut(s) 779, 824
Bst4CI ACNGT 4 cut(s) 63, 341, 1327, 2495
Bst6I CTCTTC 5 cut(s) 302, 381, 515, 1304, 2283
BstAFI CTTAAG 2 cut(s) 1217, 1823
BstAUI TGTACA 1 cut(s) 63
BstBI TTCGAA 1 cut(s) 1276
BstC8I GCNNGC 3 cut(s) 273, 1016, 1893
BstDEI CTNAG 9 cut(s) 117, 204, 614, 938, 1346, 1580, 1976, 2294, 2478
BstDSI CCRYGG 1 cut(s) 1256
BstENI CCTNNNNNAGG 1 cut(s) 442
BstF5I GGATG 6 cut(s) 832, 919, 972, 976, 2149, 2204
BstFNI CGCG 1 cut(s) 2425
BstHHI GCGC 3 cut(s) 277, 556, 892
BstKTI GATC 6 cut(s) 1084, 1240, 1519, 1873, 1936, 1972
BstMAI GTCTC 3 cut(s) 48, 1847, 2362
BstMBI GATC 6 cut(s) 1081, 1237, 1516, 1870, 1933, 1969
BstMCI CGRYCG 1 cut(s) 2429
BstMWI GCNNNNNNNGC 3 cut(s) 218, 1385, 1853
BstNI CCWGG 2 cut(s) 779, 824
BstNSI RCATGY 3 cut(s) 881, 1197, 1652
BstPAI GACNNNNGTC 1 cut(s) 2431
BstSCI CCNGG 2 cut(s) 777, 822
BstSFI CTRYAG 1 cut(s) 2303
BstUI CGCG 1 cut(s) 2425
BstV1I GCAGC 8 cut(s) 182, 185, 224, 227, 252, 829, 2002, 2101
BstV2I GAAGAC 2 cut(s) 2130, 2457
BstX2I RGATCY 3 cut(s) 1081, 1870, 1933
BstXI CCANNNNNNTGG 2 cut(s) 759, 1701
BstYI RGATCY 3 cut(s) 1081, 1870, 1933
BstZ17I GTATAC 2 cut(s) 1199, 2259
BsuI GTATCC 1 cut(s) 1390
BsuRI GGCC 3 cut(s) 90, 1379, 1725
BtgI CCRYGG 1 cut(s) 1256
BtrI CACGTC 1 cut(s) 412
BtsCI GGATG 6 cut(s) 832, 919, 972, 976, 2149, 2204
BtsI GCAGTG 2 cut(s) 93, 1902
BtsIMutI CAGTG 6 cut(s) 93, 160, 1016, 1902, 2015, 2397
BtuMI TCGCGA 1 cut(s) 2425
BveI ACCTGC 1 cut(s) 2431
Cac8I GCNNGC 3 cut(s) 273, 1016, 1893
CciI TCATGA 1 cut(s) 109
CfoI GCGC 3 cut(s) 277, 556, 892
Cfr13I GGNCC 2 cut(s) 135, 1704
CseI GACGC 4 cut(s) 499, 728, 1538, 2351
Csp6I GTAC 7 cut(s) 64, 309, 1228, 1242, 1328, 1615, 2071
CspCI CAANNNNNGTGG 2 cut(s) 2262, 2297
CviQI GTAC 7 cut(s) 64, 309, 1228, 1242, 1328, 1615, 2071
DdeI CTNAG 9 cut(s) 117, 204, 614, 938, 1346, 1580, 1976, 2294, 2478
DpnI GATC 6 cut(s) 1083, 1239, 1518, 1872, 1935, 1971
DpnII GATC 6 cut(s) 1081, 1237, 1516, 1870, 1933, 1969
DraI TTTAAA 2 cut(s) 1249, 1656
DrdI GACNNNNNNGTC 1 cut(s) 2318
DseDI GACNNNNNNGTC 1 cut(s) 2318
Eam1104I CTCTTC 5 cut(s) 302, 381, 515, 1304, 2283
EarI CTCTTC 5 cut(s) 302, 381, 515, 1304, 2283
Ecl136II GAGCTC 1 cut(s) 2293
Eco130I CCWWGG 2 cut(s) 1019, 1726
Eco147I AGGCCT 1 cut(s) 1725
Eco24I GRGCYC 3 cut(s) 365, 527, 2295
Eco32I GATATC 1 cut(s) 978
Eco47I GGWCC 2 cut(s) 135, 1704
Eco53kI GAGCTC 1 cut(s) 2293
Eco57I CTGAAG 4 cut(s) 822, 931, 1454, 2436
EcoICRI GAGCTC 1 cut(s) 2293
EcoNI CCTNNNNNAGG 1 cut(s) 442
EcoRII CCWGG 2 cut(s) 777, 822
EcoRV GATATC 1 cut(s) 978
EcoT14I CCWWGG 2 cut(s) 1019, 1726
EcoT38I GRGCYC 3 cut(s) 365, 527, 2295
ErhI CCWWGG 2 cut(s) 1019, 1726
FalI AAGNNNNNCTT 2 cut(s) 2324, 2356
FaqI GGGAC 1 cut(s) 1690
FauI CCCGC 1 cut(s) 1884
FauNDI CATATG 3 cut(s) 255, 291, 794
FblI GTMKAC 3 cut(s) 1198, 2258, 2302
FokI GGATG 6 cut(s) 839, 926, 979, 983, 2156, 2191
FriOI GRGCYC 3 cut(s) 365, 527, 2295
FspBI CTAG 2 cut(s) 449, 594
FspI TGCGCA 3 cut(s) 276, 555, 891
GlaI GCGC 3 cut(s) 276, 555, 891
GsaI CCCAGC 2 cut(s) 1711, 1997
GsuI CTGGAG 2 cut(s) 761, 1071
HaeIII GGCC 3 cut(s) 90, 1379, 1725
HapII CCGG 2 cut(s) 320, 2429
HgaI GACGC 4 cut(s) 499, 728, 1538, 2351
HhaI GCGC 3 cut(s) 277, 556, 892
Hin6I GCGC 3 cut(s) 275, 554, 890
HinP1I GCGC 3 cut(s) 275, 554, 890
HincII GTYRAC 4 cut(s) 1323, 1476, 2101, 2323
HindII GTYRAC 4 cut(s) 1323, 1476, 2101, 2323
HindIII AAGCTT 3 cut(s) 77, 1232, 1457
HinfI GANTC 9 cut(s) 677, 1278, 1573, 1582, 1623, 1907, 2236, 2420, 2432
HpaII CCGG 2 cut(s) 320, 2429
HphI GGTGA 5 cut(s) 664, 741, 1789, 2035, 2206
Hpy166II GTNNAC 7 cut(s) 1199, 1323, 1476, 2101, 2259, 2303, 2323
Hpy8I GTNNAC 7 cut(s) 1199, 1323, 1476, 2101, 2259, 2303, 2323
Hpy99I CGWCG 1 cut(s) 2367
HpyAV CCTTC 7 cut(s) 202, 1072, 1146, 1352, 1715, 1978, 2302
HpyCH4III ACNGT 4 cut(s) 63, 341, 1327, 2495
HpyCH4IV ACGT 1 cut(s) 411
HpyF10VI GCNNNNNNNGC 3 cut(s) 218, 1385, 1853
HpyF3I CTNAG 9 cut(s) 117, 204, 614, 938, 1346, 1580, 1976, 2294, 2478
HpySE526I ACGT 1 cut(s) 411
HspAI GCGC 3 cut(s) 275, 554, 890
Kzo9I GATC 6 cut(s) 1081, 1237, 1516, 1870, 1933, 1969
LguI GCTCTTC 4 cut(s) 302, 381, 515, 2283
LmnI GCTCC 4 cut(s) 814, 1546, 1852, 1987
Lsp1109I GCAGC 8 cut(s) 182, 185, 224, 227, 252, 829, 2002, 2101
LweI GCATC 4 cut(s) 553, 1117, 1809, 1843
MaeI CTAG 2 cut(s) 449, 594
MaeII ACGT 1 cut(s) 411
MaeIII GTNAC 4 cut(s) 341, 747, 1029, 2012
MalI GATC 6 cut(s) 1083, 1239, 1518, 1872, 1935, 1971
MbiI CCGCTC 1 cut(s) 1891
MboI GATC 6 cut(s) 1081, 1237, 1516, 1870, 1933, 1969
MflI RGATCY 3 cut(s) 1081, 1870, 1933
MhlI GDGCHC 4 cut(s) 365, 393, 527, 2295
MlyI GAGTC 4 cut(s) 671, 1591, 1901, 2441
MmeI TCCRAC 4 cut(s) 211, 1214, 2046, 2345
MroXI GAANNNNTTC 1 cut(s) 933
MslI CAYNNNNRTG 3 cut(s) 757, 965, 1568
MspA1I CMGCKG 4 cut(s) 218, 1466, 1993, 2092
MspCI CTTAAG 2 cut(s) 1217, 1823
MspI CCGG 2 cut(s) 320, 2429
MspR9I CCNGG 2 cut(s) 779, 824
Mva1269I GAATGC 1 cut(s) 2376
MvaI CCWGG 2 cut(s) 779, 824
MvnI CGCG 1 cut(s) 2425
MwoI GCNNNNNNNGC 3 cut(s) 218, 1385, 1853
NdeI CATATG 3 cut(s) 255, 291, 794
NdeII GATC 6 cut(s) 1081, 1237, 1516, 1870, 1933, 1969
NlaIV GGNNCC 5 cut(s) 136, 193, 436, 1083, 1706
NmuCI GTSAC 3 cut(s) 747, 1029, 2012
NruI TCGCGA 1 cut(s) 2425
NsbI TGCGCA 3 cut(s) 276, 555, 891
NspI RCATGY 3 cut(s) 881, 1197, 1652
NspV TTCGAA 1 cut(s) 1276
PagI TCATGA 1 cut(s) 109
PceI AGGCCT 1 cut(s) 1725
PciI ACATGT 1 cut(s) 1193
PciSI GCTCTTC 4 cut(s) 302, 381, 515, 2283
PctI GAATGC 1 cut(s) 2376
PdmI GAANNNNTTC 1 cut(s) 933
PfeI GAWTC 5 cut(s) 1278, 1573, 1623, 2236, 2420
PfoI TCCNGGA 1 cut(s) 777
PleI GAGTC 4 cut(s) 671, 1590, 1901, 2440
PpsI GAGTC 4 cut(s) 671, 1590, 1901, 2440
PscI ACATGT 1 cut(s) 1193
PshAI GACNNNNGTC 1 cut(s) 2431
PshBI ATTAAT 1 cut(s) 1928
Psp124BI GAGCTC 1 cut(s) 2295
Psp6I CCWGG 2 cut(s) 777, 822
PspFI CCCAGC 2 cut(s) 1707, 1993
PspGI CCWGG 2 cut(s) 777, 822
PspN4I GGNNCC 5 cut(s) 136, 193, 436, 1083, 1706
PspPI GGNCC 2 cut(s) 135, 1704
PsuI RGATCY 3 cut(s) 1081, 1870, 1933
PvuII CAGCTG 2 cut(s) 1466, 1993
RruI TCGCGA 1 cut(s) 2425
RsaI GTAC 7 cut(s) 65, 310, 1229, 1243, 1329, 1616, 2072
RsaNI GTAC 7 cut(s) 64, 309, 1228, 1242, 1328, 1615, 2071
RseI CAYNNNNRTG 3 cut(s) 757, 965, 1568
SacI GAGCTC 1 cut(s) 2295
SapI GCTCTTC 4 cut(s) 302, 381, 515, 2283
Sau3AI GATC 6 cut(s) 1081, 1237, 1516, 1870, 1933, 1969
Sau96I GGNCC 2 cut(s) 135, 1704
SchI GAGTC 4 cut(s) 671, 1591, 1901, 2441
ScrFI CCNGG 2 cut(s) 779, 824
SduI GDGCHC 4 cut(s) 365, 393, 527, 2295
SfaNI GCATC 4 cut(s) 553, 1117, 1809, 1843
SfcI CTRYAG 1 cut(s) 2303
SfuI TTCGAA 1 cut(s) 1276
SinI GGWCC 2 cut(s) 135, 1704
SmiMI CAYNNNNRTG 3 cut(s) 757, 965, 1568
SmlI CTYRAG 3 cut(s) 80, 1217, 1823
SmoI CTYRAG 3 cut(s) 80, 1217, 1823
SseBI AGGCCT 1 cut(s) 1725
SsiI CCGC 3 cut(s) 218, 1891, 2092
SspI AATATT 2 cut(s) 533, 664
SspMI CTAG 2 cut(s) 449, 594
SstI GAGCTC 1 cut(s) 2295
StuI AGGCCT 1 cut(s) 1725
StyD4I CCNGG 2 cut(s) 777, 822
StyI CCWWGG 2 cut(s) 1019, 1726
TaaI ACNGT 4 cut(s) 63, 341, 1327, 2495
TaiI ACGT 1 cut(s) 414
TaqI TCGA 4 cut(s) 147, 1276, 1338, 1956
TatI WGTACW 4 cut(s) 63, 308, 1327, 2070
TauI GCSGC 1 cut(s) 221
TfiI GAWTC 5 cut(s) 1278, 1573, 1623, 2236, 2420
TscAI CASTG 6 cut(s) 100, 160, 1016, 1902, 2015, 2404
TseFI GTSAC 3 cut(s) 747, 1029, 2012
TseI GCWGC 9 cut(s) 9, 170, 173, 212, 215, 240, 817, 1990, 2089
Tsp45I GTSAC 3 cut(s) 747, 1029, 2012
TspGWI ACGGA 1 cut(s) 1047
TspRI CASTG 6 cut(s) 100, 160, 1016, 1902, 2015, 2404
Vha464I CTTAAG 2 cut(s) 1217, 1823
VpaK11BI GGWCC 2 cut(s) 135, 1704
VspI ATTAAT 1 cut(s) 1928
XagI CCTNNNNNAGG 1 cut(s) 442
XapI RAATTY 3 cut(s) 853, 1498, 2200
XceI RCATGY 3 cut(s) 881, 1197, 1652
XmiI GTMKAC 3 cut(s) 1198, 2258, 2302
XmnI GAANNNNTTC 1 cut(s) 933
XspI CTAG 2 cut(s) 449, 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.