Rw6G003730

Domain of unknown function (DUF4598)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0010025
Physical Location & Seq
Reverse (-)
6138873 .. 6140864
1992 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G003730.1

Sequence Viewer

Length: 615 bp
ATGGGAACTCCAAGCAAAGATCTTCTGCAATTGGAGCACAACAAGTCCTCAATTTCACCGCTGGAATCCACACTACTTGTTTGTAAAAATGAATCCCAGCCTGCCCAAACAAAGAAGCCCATCATTGCTCCTCCTCCCAAAAGCCAGTTGTTGGGAAAAATGAGGAACTTCTTGGGAGTCATGTCAGAAGCTAATGAAAAATTGCAGCTTGATGCGAAGGATAATCCAGAAAAATATGACATCGAAGCGCTCACTGGAGATGAGTCTCAAGTCGTTAGTGTGGAATGTATGTTGGGTGTTGTTGATCTTCAAACTCCTGAGGCTGTGGCTGCAGCAGAAGCTGCAGTTTCTGGTTATCAGCCTGTGATACCTGTGGAGGATAGCAGTAGTGGAGAGGAATCAGATGATAGTAGCGGTGATGATGAAGAAGAAAGTAGCGATGACAATGAAGATGATGAAAGCAATGAGGATAAGAAAACATCCTCTCCGGACAAAATTAATTCTTTAAGTGAAGAATTTAATAATAAGCGGCCCAAAAGCGGCCTAAAATTATTGAGCTTTCATAACAATCTTGTGAAAACTAGTTCTCAAGAATGTACCACTGATTTATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4598

Protein Analysis

204

Amino Acids

22.1

Weight (kDa)

4.3

Isoelectric Point (pI)

73.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NOPCHAP1 PF15370 50 - 156 2.8e-10 NOP protein chaperone 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 151
AccIII TCCGGA 1 cut(s) 487
AciI CCGC 4 cut(s) 59, 414, 529, 540
AcsI RAATTY 1 cut(s) 515
AfaI GTAC 1 cut(s) 598
AfeI AGCGCT 1 cut(s) 249
AfiI CCNNNNNNNGG 2 cut(s) 151, 539
AgsI TTSAA 1 cut(s) 311
AhlI ACTAGT 1 cut(s) 581
AluBI AGCT 4 cut(s) 191, 208, 341, 558
AluI AGCT 4 cut(s) 191, 208, 341, 558
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 1 cut(s) 270
AlwNI CAGNNNCTG 2 cut(s) 341, 350
Aor13HI TCCGGA 1 cut(s) 487
Aor51HI AGCGCT 1 cut(s) 249
AoxI GGCC 2 cut(s) 530, 541
ApeKI GCWGC 4 cut(s) 205, 329, 332, 341
ApoI RAATTY 1 cut(s) 515
AseI ATTAAT 1 cut(s) 498
AspLEI GCGC 1 cut(s) 250
AspS9I GGNCC 1 cut(s) 531
AsuHPI GGTGA 2 cut(s) 48, 428
AxyI CCTNAGG 1 cut(s) 318
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 4 cut(s) 217, 316, 328, 344
BccI CCATC 1 cut(s) 128
BcoDI GTCTC 1 cut(s) 270
BcuI ACTAGT 1 cut(s) 581
BfaI CTAG 1 cut(s) 582
BfmI CTRYAG 2 cut(s) 330, 342
BfoI RGCGCY 1 cut(s) 251
BglII AGATCT 1 cut(s) 19
BisI GCNGC 6 cut(s) 206, 330, 333, 342, 530, 541
BlsI GCNGC 6 cut(s) 207, 331, 334, 343, 531, 542
BmgT120I GGNCC 1 cut(s) 531
BmsI GCATC 1 cut(s) 202
BpmI CTGGAG 1 cut(s) 276
BpuEI CTTGAG 2 cut(s) 252, 573
BsaWI WCCGGW 1 cut(s) 487
BsaXI ACNNNNNCTCC 2 cut(s) 469, 499
Bsc4I CCNNNNNNNGG 2 cut(s) 151, 539
Bse1I ACTGG 2 cut(s) 145, 259
Bse21I CCTNAGG 1 cut(s) 318
Bse3DI GCAATG 2 cut(s) 123, 469
BseAI TCCGGA 1 cut(s) 487
BseGI GGATG 1 cut(s) 479
BseLI CCNNNNNNNGG 2 cut(s) 151, 539
BseMI GCAATG 2 cut(s) 123, 469
BseMII CTCAG 1 cut(s) 309
BseNI ACTGG 2 cut(s) 145, 259
BseRI GAGGAG 2 cut(s) 120, 123
BseXI GCAGC 4 cut(s) 217, 316, 328, 344
BseYI CCCAGC 1 cut(s) 96
BshFI GGCC 2 cut(s) 532, 543
BsiHKAI GWGCWC 1 cut(s) 39
BsiSI CCGG 1 cut(s) 488
BslI CCNNNNNNNGG 2 cut(s) 151, 539
BsmAI GTCTC 1 cut(s) 270
BsnI GGCC 2 cut(s) 532, 543
Bsp1286I GDGCHC 1 cut(s) 39
Bsp13I TCCGGA 1 cut(s) 487
Bsp143I GATC 2 cut(s) 19, 304
BspACI CCGC 4 cut(s) 59, 414, 529, 540
BspANI GGCC 2 cut(s) 532, 543
BspCNI CTCAG 1 cut(s) 310
BspEI TCCGGA 1 cut(s) 487
BspMAI CTGCAG 2 cut(s) 334, 346
BsrDI GCAATG 2 cut(s) 123, 469
BsrI ACTGG 2 cut(s) 145, 259
BssMI GATC 2 cut(s) 19, 304
BstAPI GCANNNNNTGC 1 cut(s) 341
BstC8I GCNNGC 1 cut(s) 102
BstDEI CTNAG 1 cut(s) 318
BstF5I GGATG 1 cut(s) 479
BstH2I RGCGCY 1 cut(s) 251
BstHHI GCGC 1 cut(s) 250
BstKTI GATC 2 cut(s) 22, 307
BstMAI GTCTC 1 cut(s) 270
BstMBI GATC 2 cut(s) 19, 304
BstMWI GCNNNNNNNGC 4 cut(s) 34, 329, 338, 341
BstSFI CTRYAG 2 cut(s) 330, 342
BstV1I GCAGC 4 cut(s) 217, 316, 328, 344
BstX2I RGATCY 1 cut(s) 19
BstYI RGATCY 1 cut(s) 19
Bsu36I CCTNAGG 1 cut(s) 318
BsuRI GGCC 2 cut(s) 532, 543
BtgZI GCGATG 1 cut(s) 453
BtsCI GGATG 1 cut(s) 479
BtsIMutI CAGTG 2 cut(s) 252, 600
Cac8I GCNNGC 1 cut(s) 102
CaiI CAGNNNCTG 2 cut(s) 341, 350
CfoI GCGC 1 cut(s) 250
Cfr13I GGNCC 1 cut(s) 531
Csp6I GTAC 1 cut(s) 597
CspCI CAANNNNNGTGG 2 cut(s) 58, 93
CviAII CATG 1 cut(s) 181
CviQI GTAC 1 cut(s) 597
DdeI CTNAG 1 cut(s) 318
DpnI GATC 2 cut(s) 21, 306
DpnII GATC 2 cut(s) 19, 304
Eco47III AGCGCT 1 cut(s) 249
Eco81I CCTNAGG 1 cut(s) 318
FaeI CATG 1 cut(s) 184
FaiI YATR 5 cut(s) 182, 237, 290, 564, 613
FatI CATG 1 cut(s) 180
Fnu4HI GCNGC 6 cut(s) 206, 330, 333, 342, 530, 541
FokI GGATG 1 cut(s) 466
Fsp4HI GCNGC 6 cut(s) 206, 330, 333, 342, 530, 541
FspBI CTAG 1 cut(s) 582
GlaI GCGC 1 cut(s) 249
GluI GCNGC 6 cut(s) 206, 330, 333, 342, 530, 541
GsaI CCCAGC 1 cut(s) 100
GsuI CTGGAG 1 cut(s) 276
HaeII RGCGCY 1 cut(s) 251
HaeIII GGCC 2 cut(s) 532, 543
HapII CCGG 1 cut(s) 488
HhaI GCGC 1 cut(s) 250
Hin1II CATG 1 cut(s) 184
Hin6I GCGC 1 cut(s) 248
HinP1I GCGC 1 cut(s) 248
HinfI GANTC 5 cut(s) 65, 92, 177, 263, 398
HpaII CCGG 1 cut(s) 488
HphI GGTGA 2 cut(s) 48, 428
Hpy188I TCNGA 2 cut(s) 187, 403
Hpy188III TCNNGA 4 cut(s) 227, 317, 488, 590
HpyAV CCTTC 1 cut(s) 211
HpyCH4V TGCA 4 cut(s) 28, 205, 332, 344
HpyF10VI GCNNNNNNNGC 4 cut(s) 34, 329, 338, 341
HpyF3I CTNAG 1 cut(s) 318
Hsp92II CATG 1 cut(s) 184
HspAI GCGC 1 cut(s) 248
Kpn2I TCCGGA 1 cut(s) 487
Kzo9I GATC 2 cut(s) 19, 304
LmnI GCTCC 2 cut(s) 34, 133
Lsp1109I GCAGC 4 cut(s) 217, 316, 328, 344
LweI GCATC 1 cut(s) 202
MaeI CTAG 1 cut(s) 582
MalI GATC 2 cut(s) 21, 306
MboI GATC 2 cut(s) 19, 304
MboII GAAGA 6 cut(s) 14, 299, 437, 440, 461, 524
MfeI CAATTG 1 cut(s) 29
MflI RGATCY 1 cut(s) 19
MhlI GDGCHC 1 cut(s) 39
MluCI AATT 7 cut(s) 29, 51, 200, 495, 499, 515, 548
MlyI GAGTC 2 cut(s) 186, 272
MnlI CCTC 9 cut(s) 58, 141, 144, 156, 313, 370, 388, 460, 493
MroI TCCGGA 1 cut(s) 487
MseI TTAA 3 cut(s) 498, 506, 519
MspA1I CMGCKG 1 cut(s) 61
MspI CCGG 1 cut(s) 488
MunI CAATTG 1 cut(s) 29
MwoI GCNNNNNNNGC 4 cut(s) 34, 329, 338, 341
NdeII GATC 2 cut(s) 19, 304
NlaIII CATG 1 cut(s) 184
PfeI GAWTC 3 cut(s) 65, 92, 398
PflMI CCANNNNNTGG 1 cut(s) 151
PkrI GCNGC 6 cut(s) 207, 331, 334, 343, 531, 542
PleI GAGTC 2 cut(s) 185, 271
PpsI GAGTC 2 cut(s) 185, 271
PshBI ATTAAT 1 cut(s) 498
PspFI CCCAGC 1 cut(s) 96
PspPI GGNCC 1 cut(s) 531
PstI CTGCAG 2 cut(s) 334, 346
PstNI CAGNNNCTG 2 cut(s) 341, 350
PsuI RGATCY 1 cut(s) 19
RsaI GTAC 1 cut(s) 598
RsaNI GTAC 1 cut(s) 597
SaqAI TTAA 3 cut(s) 498, 506, 519
SatI GCNGC 6 cut(s) 206, 330, 333, 342, 530, 541
Sau3AI GATC 2 cut(s) 19, 304
Sau96I GGNCC 1 cut(s) 531
SchI GAGTC 2 cut(s) 186, 272
SduI GDGCHC 1 cut(s) 39
SetI ASST 5 cut(s) 193, 210, 343, 373, 560
SfaNI GCATC 1 cut(s) 202
SfcI CTRYAG 2 cut(s) 330, 342
SmlI CTYRAG 2 cut(s) 267, 588
SmoI CTYRAG 2 cut(s) 267, 588
SpeI ACTAGT 1 cut(s) 581
Sse9I AATT 7 cut(s) 29, 51, 200, 495, 499, 515, 548
SsiI CCGC 4 cut(s) 59, 414, 529, 540
SspMI CTAG 1 cut(s) 582
TaqI TCGA 1 cut(s) 243
TasI AATT 7 cut(s) 29, 51, 200, 495, 499, 515, 548
TauI GCSGC 2 cut(s) 532, 543
TfiI GAWTC 3 cut(s) 65, 92, 398
Tru1I TTAA 3 cut(s) 498, 506, 519
Tru9I TTAA 3 cut(s) 498, 506, 519
TscAI CASTG 2 cut(s) 259, 607
TseI GCWGC 4 cut(s) 205, 329, 332, 341
TspDTI ATGAA 6 cut(s) 105, 210, 438, 462, 471, 551
TspRI CASTG 2 cut(s) 259, 607
Van91I CCANNNNNTGG 1 cut(s) 151
VspI ATTAAT 1 cut(s) 498
XapI RAATTY 1 cut(s) 515
XspI CTAG 1 cut(s) 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.