MD05G1109000.v1.1

Domain of unknown function (DUF4598)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010025
Physical Location & Seq
Forward (+)
22368715 .. 22371333
2619 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1109000.v1.1.491

Sequence Viewer

Length: 705 bp
ATGGGAAATACAAGCAAAGACCTTCTGCAATTCGAGCACAAAAGGGCTTCAACTTCACCTTTAGAAAGCACGCTACTTGTCTGTAAAAATGCCTCAACGAAGAAAGCTCAACCCGAAGGGAAACCCGGCCCTGCTCTTCCTCTTCCCAAAAGCCAAGTATTTGCTTGCTTGGGCTGTGCAGTTTTGGGACTAGTAAGGGACTTCTTGGGAGTCATATCAGAAGCTAACGAACGACTAAAGCAGGATGCAAAGGATAATCCAGAAAACTACGATATTGAAGTGCTCACTGGAAATGAATCTCAAGTCGTTAATATGGAGTTGATGCTGGGCGTTGCTGATCTTCAAACTCCTGAGGCTGTGGCTGCTGCTGAATCTGCAATTGTTGGTTGTCAGCCTGTTATACCATTGGCCATCAACAGTAGCGGAGAGGAATCAGAGGATAGCAGTGATGGTGACAGCAGCAGCGATGATAGTGACGACGAAGATGGTTCCGATGATAGTAGCAGCAATGATAATGACGACGAAGATAGTGATGAAATCAAAGATGATACAATTGATAAAGAGGATAAGAAAACAACCTCTCATGCAAGGCTCAAAACACGCAAGTCGGATAAAGATAATTCTATGAGTGATGATGCTGGTAACAATCGGTCCCAAAAACGACCGAAAATTGTTGAGCTCTCACGAAAGTCTTGTGAGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4598

Protein Analysis

235

Amino Acids

25.15

Weight (kDa)

4.38

Isoelectric Point (pI)

52.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NOPCHAP1 PF15370 65 - 171 5.2e-11 NOP protein chaperone 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 423
AcoI YGGCCR 1 cut(s) 408
AgsI TTSAA 3 cut(s) 51, 278, 344
AhlI ACTAGT 1 cut(s) 190
AluBI AGCT 3 cut(s) 107, 224, 679
AluI AGCT 3 cut(s) 107, 224, 679
Alw21I GWGCWC 3 cut(s) 39, 285, 681
AoxI GGCC 2 cut(s) 127, 408
ApeKI GCWGC 5 cut(s) 362, 365, 459, 462, 504
AspS9I GGNCC 2 cut(s) 128, 651
AsuC2I CCSGG 1 cut(s) 126
AsuHPI GGTGA 2 cut(s) 48, 464
AvaII GGWCC 1 cut(s) 651
AxyI CCTNAGG 1 cut(s) 351
BalI TGGCCA 1 cut(s) 410
BanII GRGCYC 1 cut(s) 681
Bbv12I GWGCWC 3 cut(s) 39, 285, 681
BbvI GCAGC 5 cut(s) 349, 352, 471, 474, 516
BccI CCATC 3 cut(s) 419, 443, 479
BcnI CCSGG 1 cut(s) 126
BcuI ACTAGT 1 cut(s) 190
BfaI CTAG 2 cut(s) 191, 703
BglII AGATCT 1 cut(s) 699
BisI GCNGC 5 cut(s) 363, 366, 460, 463, 505
BlsI GCNGC 5 cut(s) 364, 367, 461, 464, 506
Bme1390I CCNGG 1 cut(s) 126
Bme18I GGWCC 1 cut(s) 651
BmgT120I GGNCC 2 cut(s) 128, 651
BmiI GGNNCC 2 cut(s) 490, 653
BmrFI CCNGG 1 cut(s) 126
BmsI GCATC 3 cut(s) 235, 312, 625
BpuEI CTTGAG 1 cut(s) 285
BpuMI CCSGG 1 cut(s) 126
Bse1I ACTGG 1 cut(s) 292
Bse21I CCTNAGG 1 cut(s) 351
Bse3DI GCAATG 1 cut(s) 514
BseGI GGATG 1 cut(s) 250
BseMI GCAATG 1 cut(s) 514
BseMII CTCAG 1 cut(s) 342
BseNI ACTGG 1 cut(s) 292
BseXI GCAGC 5 cut(s) 349, 352, 471, 474, 516
BseYI CCCAGC 1 cut(s) 325
BsgI GTGCAG 1 cut(s) 198
Bsh1285I CGRYCG 1 cut(s) 665
BshFI GGCC 2 cut(s) 129, 410
BsiEI CGRYCG 1 cut(s) 665
BsiHKAI GWGCWC 3 cut(s) 39, 285, 681
BsiSI CCGG 1 cut(s) 126
BslFI GGGAC 3 cut(s) 201, 212, 637
BsmFI GGGAC 3 cut(s) 201, 212, 637
BsnI GGCC 2 cut(s) 129, 410
Bsp1286I GDGCHC 3 cut(s) 39, 285, 681
Bsp143I GATC 2 cut(s) 337, 699
BspACI CCGC 1 cut(s) 423
BspANI GGCC 2 cut(s) 129, 410
BspCNI CTCAG 1 cut(s) 343
BspLI GGNNCC 2 cut(s) 490, 653
BspQI GCTCTTC 1 cut(s) 141
BsrDI GCAATG 1 cut(s) 514
BsrI ACTGG 1 cut(s) 292
BssMI GATC 2 cut(s) 337, 699
Bst4CI ACNGT 1 cut(s) 419
Bst6I CTCTTC 2 cut(s) 141, 147
BstC8I GCNNGC 2 cut(s) 71, 166
BstDEI CTNAG 1 cut(s) 351
BstF5I GGATG 1 cut(s) 250
BstKTI GATC 2 cut(s) 340, 702
BstMBI GATC 2 cut(s) 337, 699
BstMCI CGRYCG 1 cut(s) 665
BstMWI GCNNNNNNNGC 3 cut(s) 34, 362, 374
BstSCI CCNGG 1 cut(s) 124
BstV1I GCAGC 5 cut(s) 349, 352, 471, 474, 516
BstX2I RGATCY 1 cut(s) 699
BstYI RGATCY 1 cut(s) 699
Bsu36I CCTNAGG 1 cut(s) 351
BsuRI GGCC 2 cut(s) 129, 410
BtgZI GCGATG 1 cut(s) 480
BtsCI GGATG 1 cut(s) 250
BtsI GCAGTG 1 cut(s) 451
BtsIMutI CAGTG 2 cut(s) 285, 451
Cac8I GCNNGC 2 cut(s) 71, 166
Cfr13I GGNCC 2 cut(s) 128, 651
CviAII CATG 1 cut(s) 584
DdeI CTNAG 1 cut(s) 351
DpnI GATC 2 cut(s) 339, 701
DpnII GATC 2 cut(s) 337, 699
EaeI YGGCCR 1 cut(s) 408
Eam1104I CTCTTC 2 cut(s) 141, 147
EarI CTCTTC 2 cut(s) 141, 147
Ecl136II GAGCTC 1 cut(s) 679
Eco24I GRGCYC 1 cut(s) 681
Eco47I GGWCC 1 cut(s) 651
Eco53kI GAGCTC 1 cut(s) 679
Eco81I CCTNAGG 1 cut(s) 351
EcoICRI GAGCTC 1 cut(s) 679
EcoT38I GRGCYC 1 cut(s) 681
FaeI CATG 1 cut(s) 587
FaiI YATR 5 cut(s) 215, 314, 401, 585, 626
FaqI GGGAC 3 cut(s) 201, 212, 637
FatI CATG 1 cut(s) 583
Fnu4HI GCNGC 5 cut(s) 363, 366, 460, 463, 505
FokI GGATG 1 cut(s) 257
FriOI GRGCYC 1 cut(s) 681
Fsp4HI GCNGC 5 cut(s) 363, 366, 460, 463, 505
FspBI CTAG 2 cut(s) 191, 703
GluI GCNGC 5 cut(s) 363, 366, 460, 463, 505
GsaI CCCAGC 1 cut(s) 329
HaeIII GGCC 2 cut(s) 129, 410
HapII CCGG 1 cut(s) 126
Hin1II CATG 1 cut(s) 587
HinfI GANTC 4 cut(s) 210, 296, 371, 431
HpaII CCGG 1 cut(s) 126
HphI GGTGA 2 cut(s) 48, 464
Hpy188I TCNGA 4 cut(s) 220, 436, 493, 610
Hpy188III TCNNGA 3 cut(s) 260, 350, 684
Hpy99I CGWCG 2 cut(s) 482, 524
HpyAV CCTTC 2 cut(s) 32, 110
HpyCH4III ACNGT 1 cut(s) 419
HpyCH4V TGCA 5 cut(s) 28, 179, 248, 377, 587
HpyF10VI GCNNNNNNNGC 3 cut(s) 34, 362, 374
HpyF3I CTNAG 1 cut(s) 351
Hsp92II CATG 1 cut(s) 587
Kzo9I GATC 2 cut(s) 337, 699
LguI GCTCTTC 1 cut(s) 141
LpnPI CCDG 9 cut(s) 139, 144, 227, 273, 273, 311, 363, 408, 624
Lsp1109I GCAGC 5 cut(s) 349, 352, 471, 474, 516
LweI GCATC 3 cut(s) 235, 312, 625
MaeI CTAG 2 cut(s) 191, 703
MaeIII GTNAC 3 cut(s) 452, 473, 641
MalI GATC 2 cut(s) 339, 701
MboI GATC 2 cut(s) 337, 699
MboII GAAGA 6 cut(s) 112, 128, 134, 332, 494, 536
MfeI CAATTG 2 cut(s) 378, 552
MflI RGATCY 1 cut(s) 699
MhlI GDGCHC 3 cut(s) 39, 285, 681
MlsI TGGCCA 1 cut(s) 410
MluCI AATT 5 cut(s) 29, 378, 552, 619, 669
MluNI TGGCCA 1 cut(s) 410
MlyI GAGTC 1 cut(s) 219
MmeI TCCRAC 1 cut(s) 588
MnlI CCTC 7 cut(s) 103, 150, 346, 421, 430, 556, 589
Mox20I TGGCCA 1 cut(s) 410
MscI TGGCCA 1 cut(s) 410
MseI TTAA 1 cut(s) 309
Msp20I TGGCCA 1 cut(s) 410
MspI CCGG 1 cut(s) 126
MspR9I CCNGG 1 cut(s) 126
MunI CAATTG 2 cut(s) 378, 552
MwoI GCNNNNNNNGC 3 cut(s) 34, 362, 374
NciI CCSGG 1 cut(s) 126
NdeII GATC 2 cut(s) 337, 699
NlaIII CATG 1 cut(s) 587
NlaIV GGNNCC 2 cut(s) 490, 653
NmuCI GTSAC 2 cut(s) 452, 473
PciSI GCTCTTC 1 cut(s) 141
PfeI GAWTC 3 cut(s) 296, 371, 431
PkrI GCNGC 5 cut(s) 364, 367, 461, 464, 506
PleI GAGTC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 218
Psp124BI GAGCTC 1 cut(s) 681
PspFI CCCAGC 1 cut(s) 325
PspN4I GGNNCC 2 cut(s) 490, 653
PspPI GGNCC 2 cut(s) 128, 651
PsuI RGATCY 1 cut(s) 699
SacI GAGCTC 1 cut(s) 681
SapI GCTCTTC 1 cut(s) 141
SaqAI TTAA 1 cut(s) 309
SatI GCNGC 5 cut(s) 363, 366, 460, 463, 505
Sau3AI GATC 2 cut(s) 337, 699
Sau96I GGNCC 2 cut(s) 128, 651
SchI GAGTC 1 cut(s) 219
ScrFI CCNGG 1 cut(s) 126
SduI GDGCHC 3 cut(s) 39, 285, 681
SetI ASST 6 cut(s) 24, 61, 109, 226, 581, 681
SfaNI GCATC 3 cut(s) 235, 312, 625
SinI GGWCC 1 cut(s) 651
SmlI CTYRAG 1 cut(s) 300
SmoI CTYRAG 1 cut(s) 300
SpeI ACTAGT 1 cut(s) 190
Sse9I AATT 5 cut(s) 29, 378, 552, 619, 669
SsiI CCGC 1 cut(s) 423
SspMI CTAG 2 cut(s) 191, 703
SstI GAGCTC 1 cut(s) 681
StyD4I CCNGG 1 cut(s) 124
TaaI ACNGT 1 cut(s) 419
TaqI TCGA 1 cut(s) 33
TaqII GACCGA 2 cut(s) 639, 679
TasI AATT 5 cut(s) 29, 378, 552, 619, 669
TfiI GAWTC 3 cut(s) 296, 371, 431
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TscAI CASTG 2 cut(s) 292, 451
TseFI GTSAC 2 cut(s) 452, 473
TseI GCWGC 5 cut(s) 362, 365, 459, 462, 504
Tsp45I GTSAC 2 cut(s) 452, 473
TspDTI ATGAA 2 cut(s) 309, 549
TspRI CASTG 2 cut(s) 292, 451
VpaK11BI GGWCC 1 cut(s) 651
XspI CTAG 2 cut(s) 191, 703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.