FvH4_2g04210
BHLH Family

Transcription factor bHLH84-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0011699
Physical Location & Seq
Forward (+)
3291789 .. 3293198
1410 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g04210.t1

Sequence Viewer

Length: 810 bp
ATGGAAGATCAAGCATTTGTTCCAGAGGGATTGTACATGTTCGAGGATGAAGATTTGATCATGTCCCAGCTGCTTGCTGATTACTGTTCTGTCTCATCAAATGACCTGAATTATCAGGCTTCAAGCTCTCAAGTTTCATTTGCAAATTTTCCCAGCAGCCATGAATGTTCAACAGCAAAGGACATGGAAGGCATTGCTAATCTTTATTCTCGCAGAATTATGGCAACAAATTACCATAACCCTGAAGCTTTTCATCCGTGGGATGCCAATAGTACCACCAACTCTTTTTTCAGTCAAAGTGAAGAAAATGCAGGTATGGACCAAGTAATGAGAAATTGCAATGTGGGAGATCATGAAGTTGTGTCTGAGAATTCTAAGAAAAGGCCCTGCAGTACTTCTGTAGATGATGTCCAGAAGAACAAGAGGAATGTGAAGTCAAAGATGACAAATCAAAAGCCGGTCTTAACTAGAAATATTGCAGATAGTTGCAGCTCAGGGGATGATGAACCCATGAATGCTAATCCTCAACAGGATTTGAGTCCAAATGCGAGAAGCAGTAGGGAATTAGCAATTGATCCACAAAGAACATATGCAAAGAAAAGAAGAGACAAAATAAACTCAAGGCTGCGAATTCTGCAGAATCTTGTACCTAATGGAACAAAGGTTGATATCAGCACAATGCTGGAAGAAACTGTTGAGTATGTGAAGTTCTTACAGCTTCAGATCAAGCTTTTAAGCTCTGATGAGCTGTGGATGTACGCTCCTGTCGTTAACAACGGAATAGGCATCGGAGTTCAAGACTTGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

270

Amino Acids

30.28

Weight (kDa)

4.95

Isoelectric Point (pI)

61.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 199 - 239 6.9e-09 Helix-loop-helix DNA-binding domain
bHLH_SAC51 PF23173 200 - 246 1e-06 Transcription factor SAC51 bHLH domain
bHLH_LHW PF23176 202 - 239 3.5e-06 Transcription factor LHW bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011699)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14760 AT2G14760 AT2G14760 AT4G33880
fragaria_vesca FvH4_2g04210
malus_domestica MD05G1098800.v1.1 MD10G1104600.v1.1
prunus_persica Prupe.8G143500_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0253631
rosa_laevigata RLG00000014998
rosa_multiflora Rmu_sc0000362.1_g000001 Rmu_sc0002405.1_g000005
rosa_roxburghii Rroxscaffold_7G00211750
rosa_rugosa Rorug05G0545200
rosa_samantha Rh6AG060800 Rh6BG054600 Rh6CG054200 Rh6DG051700
rosa_wichuraiana Rw6G005400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 302
AclWI GGATC 1 cut(s) 569
AcsI RAATTY 3 cut(s) 145, 370, 630
AcuI CTGAAG 2 cut(s) 264, 704
AfaI GTAC 5 cut(s) 35, 274, 394, 648, 758
AflIII ACRYGT 1 cut(s) 36
AgsI TTSAA 3 cut(s) 123, 171, 797
AjuI GAANNNNNNNTTGG 2 cut(s) 272, 304
AluBI AGCT 8 cut(s) 70, 126, 248, 492, 718, 730, 738, 748
AluI AGCT 8 cut(s) 70, 126, 248, 492, 718, 730, 738, 748
Alw26I GTCTC 2 cut(s) 97, 600
AlwI GGATC 1 cut(s) 569
AoxI GGCC 1 cut(s) 383
ApeKI GCWGC 4 cut(s) 70, 156, 489, 625
ApoI RAATTY 3 cut(s) 145, 370, 630
Asp700I GAANNNNTTC 1 cut(s) 249
AspS9I GGNCC 2 cut(s) 319, 384
AvaII GGWCC 1 cut(s) 319
BbvI GCAGC 4 cut(s) 57, 168, 501, 612
BclI TGATCA 1 cut(s) 57
BcoDI GTCTC 2 cut(s) 97, 600
BfaI CTAG 1 cut(s) 468
BfmI CTRYAG 3 cut(s) 388, 399, 635
BfuAI ACCTGC 1 cut(s) 302
BisI GCNGC 4 cut(s) 71, 157, 490, 626
BlsI GCNGC 4 cut(s) 72, 158, 491, 627
BmcAI AGTACT 1 cut(s) 394
Bme18I GGWCC 1 cut(s) 319
BmgT120I GGNCC 2 cut(s) 319, 384
BmsI GCATC 2 cut(s) 253, 795
Bpu10I CCTNAGC 1 cut(s) 493
BpuEI CTTGAG 2 cut(s) 114, 604
BsaJI CCNNGG 1 cut(s) 257
Bse118I RCCGGY 1 cut(s) 457
Bse3DI GCAATG 2 cut(s) 192, 346
BseDI CCNNGG 1 cut(s) 257
BseGI GGATG 5 cut(s) 52, 253, 268, 505, 759
BseMI GCAATG 2 cut(s) 192, 346
BseMII CTCAG 2 cut(s) 357, 507
BseXI GCAGC 4 cut(s) 57, 168, 501, 612
BseYI CCCAGC 2 cut(s) 66, 152
BshFI GGCC 1 cut(s) 385
BsiSI CCGG 1 cut(s) 458
BslFI GGGAC 1 cut(s) 49
BsmAI GTCTC 2 cut(s) 97, 600
BsmFI GGGAC 1 cut(s) 49
BsmI GAATGC 1 cut(s) 520
BsnI GGCC 1 cut(s) 385
Bsp1407I TGTACA 1 cut(s) 33
Bsp143I GATC 5 cut(s) 7, 57, 349, 574, 723
BspANI GGCC 1 cut(s) 385
BspCNI CTCAG 2 cut(s) 358, 506
BspHI TCATGA 1 cut(s) 352
BspMAI CTGCAG 2 cut(s) 392, 639
BspMI ACCTGC 1 cut(s) 302
BspPI GGATC 1 cut(s) 569
BsrDI GCAATG 2 cut(s) 192, 346
BsrFI RCCGGY 1 cut(s) 457
BsrGI TGTACA 1 cut(s) 33
BssAI RCCGGY 1 cut(s) 457
BssECI CCNNGG 1 cut(s) 257
BssMI GATC 5 cut(s) 7, 57, 349, 574, 723
Bst4CI ACNGT 2 cut(s) 86, 694
Bst6I CTCTTC 1 cut(s) 598
BstAUI TGTACA 1 cut(s) 33
BstC8I GCNNGC 1 cut(s) 75
BstDEI CTNAG 3 cut(s) 366, 375, 493
BstDSI CCRYGG 1 cut(s) 257
BstF5I GGATG 5 cut(s) 52, 253, 268, 505, 759
BstKTI GATC 5 cut(s) 10, 60, 352, 577, 726
BstMAI GTCTC 2 cut(s) 97, 600
BstMBI GATC 5 cut(s) 7, 57, 349, 574, 723
BstMWI GCNNNNNNNGC 1 cut(s) 634
BstNSI RCATGY 1 cut(s) 40
BstSFI CTRYAG 3 cut(s) 388, 399, 635
BstV1I GCAGC 4 cut(s) 57, 168, 501, 612
BsuRI GGCC 1 cut(s) 385
BtgI CCRYGG 1 cut(s) 257
BtsCI GGATG 5 cut(s) 52, 253, 268, 505, 759
BveI ACCTGC 1 cut(s) 302
Cac8I GCNNGC 1 cut(s) 75
CciI TCATGA 1 cut(s) 352
Cfr10I RCCGGY 1 cut(s) 457
Cfr13I GGNCC 2 cut(s) 319, 384
Csp6I GTAC 5 cut(s) 34, 273, 393, 647, 757
CviAII CATG 6 cut(s) 37, 61, 161, 184, 353, 511
CviQI GTAC 5 cut(s) 34, 273, 393, 647, 757
DdeI CTNAG 3 cut(s) 366, 375, 493
DpnI GATC 5 cut(s) 9, 59, 351, 576, 725
DpnII GATC 5 cut(s) 7, 57, 349, 574, 723
Eam1104I CTCTTC 1 cut(s) 598
EarI CTCTTC 1 cut(s) 598
Eco32I GATATC 1 cut(s) 670
Eco47I GGWCC 1 cut(s) 319
Eco57I CTGAAG 2 cut(s) 264, 704
EcoO109I RGGNCCY 1 cut(s) 384
EcoRI GAATTC 2 cut(s) 370, 630
EcoRV GATATC 1 cut(s) 670
FaeI CATG 6 cut(s) 40, 64, 164, 187, 356, 514
FalI AAGNNNNNCTT 2 cut(s) 446, 478
FaqI GGGAC 1 cut(s) 49
FatI CATG 6 cut(s) 36, 60, 160, 183, 352, 510
FauNDI CATATG 1 cut(s) 589
FbaI TGATCA 1 cut(s) 57
Fnu4HI GCNGC 4 cut(s) 71, 157, 490, 626
FokI GGATG 5 cut(s) 59, 240, 275, 512, 766
Fsp4HI GCNGC 4 cut(s) 71, 157, 490, 626
FspBI CTAG 1 cut(s) 468
GluI GCNGC 4 cut(s) 71, 157, 490, 626
GsaI CCCAGC 2 cut(s) 70, 156
HaeIII GGCC 1 cut(s) 385
HapII CCGG 1 cut(s) 458
Hin1II CATG 6 cut(s) 40, 64, 164, 187, 356, 514
HincII GTYRAC 1 cut(s) 772
HindII GTYRAC 1 cut(s) 772
HindIII AAGCTT 2 cut(s) 246, 728
HinfI GANTC 2 cut(s) 538, 640
HpaI GTTAAC 1 cut(s) 772
HpaII CCGG 1 cut(s) 458
Hpy166II GTNNAC 1 cut(s) 772
Hpy188I TCNGA 5 cut(s) 367, 723, 742, 791, 809
Hpy188III TCNNGA 4 cut(s) 23, 353, 412, 797
Hpy8I GTNNAC 1 cut(s) 772
HpyAV CCTTC 1 cut(s) 182
HpyCH4III ACNGT 2 cut(s) 86, 694
HpyCH4V TGCA 8 cut(s) 143, 311, 339, 390, 479, 489, 593, 637
HpyF10VI GCNNNNNNNGC 1 cut(s) 634
HpyF3I CTNAG 3 cut(s) 366, 375, 493
Hsp92II CATG 6 cut(s) 40, 64, 164, 187, 356, 514
Ksp22I TGATCA 1 cut(s) 57
KspAI GTTAAC 1 cut(s) 772
Kzo9I GATC 5 cut(s) 7, 57, 349, 574, 723
LmnI GCTCC 1 cut(s) 766
Lsp1109I GCAGC 4 cut(s) 57, 168, 501, 612
LweI GCATC 2 cut(s) 253, 795
MaeI CTAG 1 cut(s) 468
MalI GATC 5 cut(s) 9, 59, 351, 576, 725
MboI GATC 5 cut(s) 7, 57, 349, 574, 723
MboII GAAGA 6 cut(s) 17, 62, 314, 427, 615, 698
MfeI CAATTG 1 cut(s) 570
MluCI AATT 9 cut(s) 109, 145, 216, 229, 334, 370, 563, 570, 630
MlyI GAGTC 1 cut(s) 547
MnlI CCTC 4 cut(s) 19, 37, 417, 534
MroXI GAANNNNTTC 1 cut(s) 249
MseI TTAA 3 cut(s) 464, 734, 771
MspA1I CMGCKG 1 cut(s) 70
MspI CCGG 1 cut(s) 458
MunI CAATTG 1 cut(s) 570
Mva1269I GAATGC 1 cut(s) 520
MwoI GCNNNNNNNGC 1 cut(s) 634
NdeI CATATG 1 cut(s) 589
NdeII GATC 5 cut(s) 7, 57, 349, 574, 723
NlaIII CATG 6 cut(s) 40, 64, 164, 187, 356, 514
NspI RCATGY 1 cut(s) 40
PagI TCATGA 1 cut(s) 352
PciI ACATGT 1 cut(s) 36
PcsI WCGNNNNNNNCGW 1 cut(s) 765
PctI GAATGC 1 cut(s) 520
PdmI GAANNNNTTC 1 cut(s) 249
PfeI GAWTC 1 cut(s) 640
PkrI GCNGC 4 cut(s) 72, 158, 491, 627
PleI GAGTC 1 cut(s) 546
PpsI GAGTC 1 cut(s) 546
PscI ACATGT 1 cut(s) 36
PspFI CCCAGC 2 cut(s) 66, 152
PspPI GGNCC 2 cut(s) 319, 384
PsrI GAACNNNNNNTAC 2 cut(s) 692, 724
PstI CTGCAG 2 cut(s) 392, 639
PvuII CAGCTG 1 cut(s) 70
RsaI GTAC 5 cut(s) 35, 274, 394, 648, 758
RsaNI GTAC 5 cut(s) 34, 273, 393, 647, 757
SaqAI TTAA 3 cut(s) 464, 734, 771
SatI GCNGC 4 cut(s) 71, 157, 490, 626
Sau3AI GATC 5 cut(s) 7, 57, 349, 574, 723
Sau96I GGNCC 2 cut(s) 319, 384
ScaI AGTACT 1 cut(s) 394
SchI GAGTC 1 cut(s) 547
SfaNI GCATC 2 cut(s) 253, 795
SfcI CTRYAG 3 cut(s) 388, 399, 635
SinI GGWCC 1 cut(s) 319
SmlI CTYRAG 2 cut(s) 129, 619
SmoI CTYRAG 2 cut(s) 129, 619
Sse9I AATT 9 cut(s) 109, 145, 216, 229, 334, 370, 563, 570, 630
SspI AATATT 1 cut(s) 475
SspMI CTAG 1 cut(s) 468
TaaI ACNGT 2 cut(s) 86, 694
TaqI TCGA 1 cut(s) 42
TasI AATT 9 cut(s) 109, 145, 216, 229, 334, 370, 563, 570, 630
TatI WGTACW 2 cut(s) 33, 392
TfiI GAWTC 1 cut(s) 640
Tru1I TTAA 3 cut(s) 464, 734, 771
Tru9I TTAA 3 cut(s) 464, 734, 771
TseI GCWGC 4 cut(s) 70, 156, 489, 625
TspDTI ATGAA 7 cut(s) 63, 126, 177, 242, 369, 519, 527
TspGWI ACGGA 2 cut(s) 246, 792
VpaK11BI GGWCC 1 cut(s) 319
XapI RAATTY 3 cut(s) 145, 370, 630
XceI RCATGY 1 cut(s) 40
XmnI GAANNNNTTC 1 cut(s) 249
XspI CTAG 1 cut(s) 468
ZrmI AGTACT 1 cut(s) 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.