MD05G1098800.v1.1
BHLH Family

Transcription factor bHLH84-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011699
Physical Location & Seq
Forward (+)
20565378 .. 20567117
1740 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1098800.v1.1.491

Sequence Viewer

Length: 984 bp
ATGGAATCTGCAGCAGCTATTGCAAATGGTCGATGGAGTTCACTTGGTGCAATGTACTCCTCCGATGAGGAGGCGGAGTTCATGTCCCAGTTGATCGGTAACTGTTCTGTCACAAATGAGATGAATGAGGCTTCAAGCTCAGCAATCCCATTTGCAATTTGTCCTAGCTATGATTTTTCAACATCTAACATGGAAGGCATTTATCAAGGTTCACAATATTCTTCAGAAATGGCTAATCTTTACCTTTCAAATGGGAGTAGTACTTATATTATGAACAATGATGAAACAAACGACTGCATTGGTAATCCTAACCGTATTGTGGAAACAAGTTACCACAACTCCGAAGCATTTCGTCCATGGGACGACAATGCTACCAACTTGTTTCCCACTCAAGGCGAAGAATATGGCATGGATCATCACCAAGAACTGAGCAATGTCAATATTGAAAACCAGCCGGGAGCTGCTATTTATGAAAAGCTTCTGCAGCTTAATAGGGAGTCTGATCATGAGATGGCAATACGGGAACCTGCAATGGAAAATGAAGTTATGGTTTCCGAAAATTCTAAGAAAAGGCCTTGCAGTTCAGTAGATGTGCTAAAGAATCAGAGGAATGTGAAGGCGAAGAAGGGCCAAAAGGCGGTCTCAAGCGGAGACATTGAAGATAATGATGCAAGCACCAAAGGGCGGAGCTCAAGCAGTTGCTGCTCAGGGGACGATGACTCCATTAATGGTTCTCATCACGATCTGAGTAGAGGCGTGAGTACTTCAATCTTGAGTCCAAAAGGGACAAAAGCCAGTAGGGGATCGGCAACTGATTCCCAAAGCATATATGCGAAGGTTGATCTCAGCACAATGCTTGAGGAGGCTATCCAGTATGTGAAGTTCTTACAGCTTCAGATAAAGCTCCTAAGCTCGGATGATATGTGGATGTTTGCTCCTATCGCGTACAATGGAATGAACATTGGGATCGACTTAAATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

328

Amino Acids

35.81

Weight (kDa)

4.55

Isoelectric Point (pI)

45.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011699)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14760 AT2G14760 AT2G14760 AT4G33880
fragaria_vesca FvH4_2g04210
malus_domestica MD05G1098800.v1.1 MD10G1104600.v1.1
prunus_persica Prupe.8G143500_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0253631
rosa_laevigata RLG00000014998
rosa_multiflora Rmu_sc0000362.1_g000001 Rmu_sc0002405.1_g000005
rosa_roxburghii Rroxscaffold_7G00211750
rosa_rugosa Rorug05G0545200
rosa_samantha Rh6AG060800 Rh6BG054600 Rh6CG054200 Rh6DG051700
rosa_wichuraiana Rw6G005400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 535
AccII CGCG 1 cut(s) 944
AciI CCGC 4 cut(s) 74, 638, 648, 685
AclWI GGATC 3 cut(s) 420, 811, 974
AcsI RAATTY 2 cut(s) 559, 976
AcuI CTGAAG 2 cut(s) 207, 878
AdeI CACNNNGTG 1 cut(s) 47
AfaI GTAC 4 cut(s) 56, 262, 763, 947
AfiI CCNNNNNNNGG 5 cut(s) 319, 392, 637, 684, 913
AgsI TTSAA 6 cut(s) 135, 180, 249, 446, 659, 768
AloI GAACNNNNNNTCC 2 cut(s) 62, 94
Alw21I GWGCWC 1 cut(s) 692
Alw26I GTCTC 2 cut(s) 645, 646
AlwI GGATC 3 cut(s) 420, 811, 974
AlwNI CAGNNNCTG 1 cut(s) 702
AoxI GGCC 2 cut(s) 572, 628
ApeKI GCWGC 5 cut(s) 11, 14, 461, 484, 702
ApoI RAATTY 2 cut(s) 559, 976
AseI ATTAAT 1 cut(s) 726
Asp700I GAANNNNTTC 2 cut(s) 348, 477
AspS9I GGNCC 1 cut(s) 628
AsuC2I CCSGG 1 cut(s) 456
AsuHPI GGTGA 1 cut(s) 410
BanII GRGCYC 1 cut(s) 692
Bbv12I GWGCWC 1 cut(s) 692
BbvI GCAGC 5 cut(s) 23, 26, 448, 496, 689
BccI CCATC 2 cut(s) 27, 505
BclI TGATCA 1 cut(s) 502
BcnI CCSGG 1 cut(s) 456
BcoDI GTCTC 2 cut(s) 645, 646
BfaI CTAG 1 cut(s) 165
BfmI CTRYAG 2 cut(s) 9, 482
BfuAI ACCTGC 1 cut(s) 535
BisI GCNGC 5 cut(s) 12, 15, 462, 485, 703
BlpI GCTNAGC 1 cut(s) 139
BlsI GCNGC 5 cut(s) 13, 16, 463, 486, 704
BmcAI AGTACT 2 cut(s) 262, 763
Bme1390I CCNGG 1 cut(s) 456
BmgT120I GGNCC 1 cut(s) 628
BmiI GGNNCC 1 cut(s) 525
BmrFI CCNGG 1 cut(s) 456
BmrI ACTGGG 1 cut(s) 82
BmsI GCATC 1 cut(s) 658
BmuI ACTGGG 1 cut(s) 82
Bpu10I CCTNAGC 2 cut(s) 706, 908
Bpu1102I GCTNAGC 1 cut(s) 139
BpuEI CTTGAG 5 cut(s) 375, 628, 676, 793, 878
BpuMI CCSGG 1 cut(s) 456
BsaI GGTCTC 1 cut(s) 646
BsaJI CCNNGG 1 cut(s) 356
BsaXI ACNNNNNCTCC 8 cut(s) 62, 68, 92, 98, 323, 353, 704, 734
Bsc4I CCNNNNNNNGG 5 cut(s) 319, 392, 637, 684, 913
Bse1I ACTGG 3 cut(s) 88, 795, 871
Bse3DI GCAATG 3 cut(s) 57, 439, 537
BseDI CCNNGG 1 cut(s) 356
BseGI GGATG 2 cut(s) 922, 933
BseLI CCNNNNNNNGG 5 cut(s) 319, 392, 637, 684, 913
BseMI GCAATG 3 cut(s) 57, 439, 537
BseMII CTCAG 5 cut(s) 153, 419, 720, 737, 859
BseNI ACTGG 3 cut(s) 88, 795, 871
BseRI GAGGAG 3 cut(s) 49, 83, 875
BseXI GCAGC 5 cut(s) 23, 26, 448, 496, 689
Bsh1236I CGCG 1 cut(s) 944
BshFI GGCC 2 cut(s) 574, 630
BsiHKAI GWGCWC 1 cut(s) 692
BsiSI CCGG 1 cut(s) 455
BslFI GGGAC 4 cut(s) 70, 374, 725, 799
BslI CCNNNNNNNGG 5 cut(s) 319, 392, 637, 684, 913
BsmAI GTCTC 2 cut(s) 645, 646
BsmFI GGGAC 4 cut(s) 70, 374, 725, 799
BsnI GGCC 2 cut(s) 574, 630
Bso31I GGTCTC 1 cut(s) 646
Bsp1286I GDGCHC 1 cut(s) 692
Bsp143I GATC 7 cut(s) 93, 412, 502, 742, 803, 841, 966
Bsp1720I GCTNAGC 1 cut(s) 139
Bsp19I CCATGG 1 cut(s) 356
BspACI CCGC 4 cut(s) 74, 638, 648, 685
BspANI GGCC 2 cut(s) 574, 630
BspCNI CTCAG 5 cut(s) 152, 420, 719, 738, 858
BspFNI CGCG 1 cut(s) 944
BspHI TCATGA 1 cut(s) 505
BspLI GGNNCC 1 cut(s) 525
BspMAI CTGCAG 2 cut(s) 13, 486
BspMI ACCTGC 1 cut(s) 535
BspPI GGATC 3 cut(s) 420, 811, 974
BspTNI GGTCTC 1 cut(s) 646
BsrDI GCAATG 3 cut(s) 57, 439, 537
BsrI ACTGG 3 cut(s) 88, 795, 871
BssECI CCNNGG 1 cut(s) 356
BssMI GATC 7 cut(s) 93, 412, 502, 742, 803, 841, 966
BssT1I CCWWGG 1 cut(s) 356
Bst4CI ACNGT 2 cut(s) 104, 314
BstAPI GCANNNNNTGC 2 cut(s) 20, 702
BstC8I GCNNGC 1 cut(s) 673
BstDEI CTNAG 7 cut(s) 139, 428, 564, 706, 746, 845, 908
BstDSI CCRYGG 1 cut(s) 356
BstF5I GGATG 2 cut(s) 922, 933
BstFNI CGCG 1 cut(s) 944
BstKTI GATC 7 cut(s) 96, 415, 505, 745, 806, 844, 969
BstMAI GTCTC 2 cut(s) 645, 646
BstMBI GATC 7 cut(s) 93, 412, 502, 742, 803, 841, 966
BstMWI GCNNNNNNNGC 4 cut(s) 20, 484, 702, 941
BstSCI CCNGG 1 cut(s) 454
BstSFI CTRYAG 2 cut(s) 9, 482
BstUI CGCG 1 cut(s) 944
BstV1I GCAGC 5 cut(s) 23, 26, 448, 496, 689
BsuRI GGCC 2 cut(s) 574, 630
BtgI CCRYGG 1 cut(s) 356
BtsCI GGATG 2 cut(s) 922, 933
BveI ACCTGC 1 cut(s) 535
Cac8I GCNNGC 1 cut(s) 673
CaiI CAGNNNCTG 1 cut(s) 702
CciI TCATGA 1 cut(s) 505
Cfr13I GGNCC 1 cut(s) 628
Csp6I GTAC 4 cut(s) 55, 261, 762, 946
CviAII CATG 5 cut(s) 82, 190, 357, 409, 506
CviQI GTAC 4 cut(s) 55, 261, 762, 946
DdeI CTNAG 7 cut(s) 139, 428, 564, 706, 746, 845, 908
DpnI GATC 7 cut(s) 95, 414, 504, 744, 805, 843, 968
DpnII GATC 7 cut(s) 93, 412, 502, 742, 803, 841, 966
DraIII CACNNNGTG 1 cut(s) 47
EciI GGCGGA 2 cut(s) 89, 700
Ecl136II GAGCTC 1 cut(s) 690
Eco130I CCWWGG 1 cut(s) 356
Eco147I AGGCCT 1 cut(s) 574
Eco24I GRGCYC 1 cut(s) 692
Eco31I GGTCTC 1 cut(s) 646
Eco53kI GAGCTC 1 cut(s) 690
Eco57I CTGAAG 2 cut(s) 207, 878
EcoICRI GAGCTC 1 cut(s) 690
EcoT14I CCWWGG 1 cut(s) 356
EcoT38I GRGCYC 1 cut(s) 692
ErhI CCWWGG 1 cut(s) 356
FaeI CATG 5 cut(s) 85, 193, 360, 412, 509
FaqI GGGAC 4 cut(s) 70, 374, 725, 799
FatI CATG 5 cut(s) 81, 189, 356, 408, 505
FbaI TGATCA 1 cut(s) 502
Fnu4HI GCNGC 5 cut(s) 12, 15, 462, 485, 703
FokI GGATG 2 cut(s) 929, 940
FriOI GRGCYC 1 cut(s) 692
Fsp4HI GCNGC 5 cut(s) 12, 15, 462, 485, 703
FspBI CTAG 1 cut(s) 165
GluI GCNGC 5 cut(s) 12, 15, 462, 485, 703
HaeIII GGCC 2 cut(s) 574, 630
HapII CCGG 1 cut(s) 455
Hin1II CATG 5 cut(s) 85, 193, 360, 412, 509
HindIII AAGCTT 1 cut(s) 476
HinfI GANTC 6 cut(s) 5, 497, 601, 719, 775, 815
HpaII CCGG 1 cut(s) 455
HphI GGTGA 1 cut(s) 410
Hpy166II GTNNAC 2 cut(s) 41, 212
Hpy188I TCNGA 9 cut(s) 64, 226, 343, 502, 556, 606, 747, 897, 916
Hpy188III TCNNGA 4 cut(s) 506, 740, 772, 981
Hpy8I GTNNAC 2 cut(s) 41, 212
HpyAV CCTTC 4 cut(s) 188, 610, 619, 829
HpyCH4III ACNGT 2 cut(s) 104, 314
HpyCH4V TGCA 9 cut(s) 11, 23, 50, 155, 297, 484, 530, 579, 671
HpyF10VI GCNNNNNNNGC 4 cut(s) 20, 484, 702, 941
HpyF3I CTNAG 7 cut(s) 139, 428, 564, 706, 746, 845, 908
Hsp92II CATG 5 cut(s) 85, 193, 360, 412, 509
Ksp22I TGATCA 1 cut(s) 502
Kzo9I GATC 7 cut(s) 93, 412, 502, 742, 803, 841, 966
LmnI GCTCC 4 cut(s) 458, 687, 909, 940
LpnPI CCDG 7 cut(s) 101, 464, 468, 540, 693, 808, 884
Lsp1109I GCAGC 5 cut(s) 23, 26, 448, 496, 689
LweI GCATC 1 cut(s) 658
MaeI CTAG 1 cut(s) 165
MaeIII GTNAC 3 cut(s) 98, 109, 329
MalI GATC 7 cut(s) 95, 414, 504, 744, 805, 843, 968
MboI GATC 7 cut(s) 93, 412, 502, 742, 803, 841, 966
MboII GAAGA 4 cut(s) 213, 410, 634, 671
MhlI GDGCHC 1 cut(s) 692
MluCI AATT 3 cut(s) 156, 559, 976
MlyI GAGTC 3 cut(s) 506, 713, 784
MnlI CCTC 8 cut(s) 61, 64, 70, 121, 600, 746, 853, 856
MroXI GAANNNNTTC 2 cut(s) 348, 477
MseI TTAA 3 cut(s) 489, 726, 974
MspI CCGG 1 cut(s) 455
MspR9I CCNGG 1 cut(s) 456
MvnI CGCG 1 cut(s) 944
MwoI GCNNNNNNNGC 4 cut(s) 20, 484, 702, 941
NciI CCSGG 1 cut(s) 456
NcoI CCATGG 1 cut(s) 356
NdeII GATC 7 cut(s) 93, 412, 502, 742, 803, 841, 966
NlaIII CATG 5 cut(s) 85, 193, 360, 412, 509
NlaIV GGNNCC 1 cut(s) 525
NmuCI GTSAC 1 cut(s) 109
PagI TCATGA 1 cut(s) 505
PceI AGGCCT 1 cut(s) 574
PdmI GAANNNNTTC 2 cut(s) 348, 477
PfeI GAWTC 3 cut(s) 5, 601, 815
PkrI GCNGC 5 cut(s) 13, 16, 463, 486, 704
PleI GAGTC 3 cut(s) 505, 713, 783
PpsI GAGTC 3 cut(s) 505, 713, 783
PshBI ATTAAT 1 cut(s) 726
Psp124BI GAGCTC 1 cut(s) 692
PspN4I GGNNCC 1 cut(s) 525
PspPI GGNCC 1 cut(s) 628
PsrI GAACNNNNNNTAC 2 cut(s) 866, 898
PstI CTGCAG 2 cut(s) 13, 486
PstNI CAGNNNCTG 1 cut(s) 702
RsaI GTAC 4 cut(s) 56, 262, 763, 947
RsaNI GTAC 4 cut(s) 55, 261, 762, 946
SacI GAGCTC 1 cut(s) 692
SaqAI TTAA 3 cut(s) 489, 726, 974
SatI GCNGC 5 cut(s) 12, 15, 462, 485, 703
Sau3AI GATC 7 cut(s) 93, 412, 502, 742, 803, 841, 966
Sau96I GGNCC 1 cut(s) 628
ScaI AGTACT 2 cut(s) 262, 763
SchI GAGTC 3 cut(s) 506, 713, 784
ScrFI CCNGG 1 cut(s) 456
SduI GDGCHC 1 cut(s) 692
SfaNI GCATC 1 cut(s) 658
SfcI CTRYAG 2 cut(s) 9, 482
SmlI CTYRAG 5 cut(s) 390, 643, 691, 772, 857
SmoI CTYRAG 5 cut(s) 390, 643, 691, 772, 857
Sse9I AATT 3 cut(s) 156, 559, 976
SseBI AGGCCT 1 cut(s) 574
SsiI CCGC 4 cut(s) 74, 638, 648, 685
SspI AATATT 2 cut(s) 218, 442
SspMI CTAG 1 cut(s) 165
SstI GAGCTC 1 cut(s) 692
StuI AGGCCT 1 cut(s) 574
StyD4I CCNGG 1 cut(s) 454
StyI CCWWGG 1 cut(s) 356
TaaI ACNGT 2 cut(s) 104, 314
TaqI TCGA 2 cut(s) 31, 969
TasI AATT 3 cut(s) 156, 559, 976
TatI WGTACW 3 cut(s) 54, 260, 761
TfiI GAWTC 3 cut(s) 5, 601, 815
Tru1I TTAA 3 cut(s) 489, 726, 974
Tru9I TTAA 3 cut(s) 489, 726, 974
TseFI GTSAC 1 cut(s) 109
TseI GCWGC 5 cut(s) 11, 14, 461, 484, 702
Tsp45I GTSAC 1 cut(s) 109
TspDTI ATGAA 7 cut(s) 70, 137, 287, 297, 486, 555, 971
VspI ATTAAT 1 cut(s) 726
XapI RAATTY 2 cut(s) 559, 976
XmnI GAANNNNTTC 2 cut(s) 348, 477
XspI CTAG 1 cut(s) 165
ZrmI AGTACT 2 cut(s) 262, 763
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.