FvH4_2g08900

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0004218
Physical Location & Seq
Forward (+)
7780847 .. 7785192
4346 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g08900.t7

Sequence Viewer

Length: 1425 bp
ATGGGTTCCTCCAACATGATCATATACGTTGGATTCTGGCTCTGGCATGCTTCTGTTCTACTGCTGCTTACTTCCAACTTTGCTCTTGCACAATCGATCATCAAGAACTTGCCAGCCTTTCATGGTGACCTCCCTTTCAAGCTTGAAACTGGGTATGTGGGCGTGGGAGATTCGGAAGAGGTGCAGCTGTTCTACTACCTTGTTGAGTCAGAAAGGAGTCCAGAAAATGATCCTCTGCTGATTTGGCTGACAGGAGGTCCTCGATGCTCTTCGTTTTATGGCCTCATCTATGAAATTGGTCCAATGTCTTTTGAACTTACAAATTTATCGACGGATCCATTAACACTAGTTCTGAATCCAAACTCATGGAACAAGGTAGCAAACATAATATTCGTAGATGCCCCTGCTGGTACTGGTTACTCCTACTCTACAACTTCCCATGGGTTAATCTCTAGTGATACGTTACATTCAACCCGGATTTATGAGTTTCTGTTAAAGTGGATTACAGCGCATCCCAAGTTTCTTGCAAATTCGTTCTATGTTGCTGGTGATTCTTACTCTGGCAAAATCGTTCCCATCATTGCCCTGCAGATAACTGATGGTATTGAAGCTGGAATCAAGCCAAATTTGAATCTCAAGGGATACATAGTTGGCAATCCAGTTTCAAATGGACAGCAGGATGTTAATTCAAGAATTGTGTATGCTCATCAGCTGGGACTTATATCAGATAGGATATATCAGTCAGCTAACAAGAATTGCAAGGGCGAATTTGTGGATCTCGATCCAAACAATAAACTGTGCCTGAACAGCCTTCAAGCAATTGATGAGTGCATTGGGAGAATTGATAATGAACATATATTGGCACCATTTTGCCTTCCAGATATCAACGAAAACCCCCAAAATTCTATTCTGGAGATGAACTGGGAGTCTAATGATGACAGTTTCTTAGACTTTTCGTTTCCTATGGTACACATGTGTAGGGAAGATAAAATTCGGTATTCTGTTCTTTGGCAAAATGATATGAATGTTCGCAATGCTCTTAACATTCGGGAGGGAACAAAGGGGCAATGGACTAAATGCAATAAAACTACTCCCTATACTTTTGACATCGCAAGTAATATTGAGTATCATCGAAATCTTAGCCAAAAGTCCCTTCGTGCTCTAATCTACAGTGGTGATCATGACATGAGTGTTCCATATATAAGTACACTAGCATGGATAGAATCTATGAACTTTACAATTGAAGAGGATTGGAGACCATGGTTCTCTAATGATCAAGTTGCAGGATACACACTTTACTATTCAAATAAAGAGTATAATTTGACATATGCGACTATCAAGGGAGGAGGACATACAGCTCCAGAATACAATCCAAAGGAATGTTTTGATATGATCAGTAGGTGGCTAGCGCATGCACCTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

475

Amino Acids

53.62

Weight (kDa)

5.06

Isoelectric Point (pI)

43.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 13 - 330 2.5e-78 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 862
AclWI GGATC 5 cut(s) 224, 329, 342, 776, 783
AcsI RAATTY 6 cut(s) 322, 529, 625, 767, 901, 990
AfaI GTAC 3 cut(s) 412, 969, 1207
AflIII ACRYGT 1 cut(s) 972
AhdI GACNNNNNGTC 1 cut(s) 255
AhlI ACTAGT 1 cut(s) 346
AjuI GAANNNNNNNTTGG 4 cut(s) 891, 923, 1137, 1169
AluBI AGCT 6 cut(s) 142, 187, 611, 712, 746, 1358
AluI AGCT 6 cut(s) 142, 187, 611, 712, 746, 1358
Alw21I GWGCWC 1 cut(s) 1162
Alw26I GTCTC 1 cut(s) 1249
AlwI GGATC 5 cut(s) 224, 329, 342, 776, 783
AoxI GGCC 1 cut(s) 280
ApeKI GCWGC 2 cut(s) 64, 184
ApoI RAATTY 6 cut(s) 322, 529, 625, 767, 901, 990
AspLEI GCGC 2 cut(s) 511, 1411
AspS9I GGNCC 2 cut(s) 257, 299
AsuC2I CCSGG 1 cut(s) 475
AsuHPI GGTGA 3 cut(s) 137, 560, 1187
AsuNHI GCTAGC 1 cut(s) 1405
AvaII GGWCC 2 cut(s) 257, 299
BaeI ACNNNNGTAYC 2 cut(s) 959, 992
BamHI GGATCC 1 cut(s) 334
BanI GGYRCC 1 cut(s) 862
Bbv12I GWGCWC 1 cut(s) 1162
BbvI GCAGC 2 cut(s) 51, 196
BccI CCATC 2 cut(s) 584, 593
BciVI GTATCC 2 cut(s) 635, 1280
BclI TGATCA 4 cut(s) 18, 1177, 1273, 1392
BcnI CCSGG 1 cut(s) 475
BcoDI GTCTC 1 cut(s) 1249
BcuI ACTAGT 1 cut(s) 346
BfaI CTAG 4 cut(s) 347, 453, 1211, 1406
BfmI CTRYAG 2 cut(s) 587, 1168
BfuI GTATCC 2 cut(s) 635, 1280
BisI GCNGC 2 cut(s) 65, 185
BlsI GCNGC 2 cut(s) 66, 186
Bme1390I CCNGG 1 cut(s) 475
Bme18I GGWCC 2 cut(s) 257, 299
BmeRI GACNNNNNGTC 1 cut(s) 255
BmgT120I GGNCC 2 cut(s) 257, 299
BmiI GGNNCC 3 cut(s) 7, 336, 864
BmrFI CCNGG 1 cut(s) 475
BmrI ACTGGG 2 cut(s) 159, 931
BmsI GCATC 3 cut(s) 254, 388, 520
BmtI GCTAGC 1 cut(s) 1409
BmuI ACTGGG 2 cut(s) 159, 931
BpmI CTGGAG 2 cut(s) 932, 1344
BpuEI CTTGAG 1 cut(s) 620
BpuMI CCSGG 1 cut(s) 475
Bsa29I ATCGAT 1 cut(s) 95
BsaBI GATNNNNATC 1 cut(s) 780
BsaI GGTCTC 1 cut(s) 1249
BsaJI CCNNGG 2 cut(s) 439, 1259
Bse1I ACTGG 4 cut(s) 154, 418, 659, 926
Bse3DI GCAATG 3 cut(s) 579, 1039, 1073
Bse8I GATNNNNATC 1 cut(s) 780
BseCI ATCGAT 1 cut(s) 95
BseDI CCNNGG 2 cut(s) 439, 1259
BseGI GGATG 2 cut(s) 511, 685
BseJI GATNNNNATC 1 cut(s) 780
BseMI GCAATG 3 cut(s) 579, 1039, 1073
BseNI ACTGG 4 cut(s) 154, 418, 659, 926
BseRI GAGGAG 1 cut(s) 1359
BseXI GCAGC 2 cut(s) 51, 196
BseYI CCCAGC 1 cut(s) 712
BsgI GTGCAG 1 cut(s) 203
BshFI GGCC 1 cut(s) 282
BshNI GGYRCC 1 cut(s) 862
BshVI ATCGAT 1 cut(s) 95
BsiHKAI GWGCWC 1 cut(s) 1162
BsiSI CCGG 1 cut(s) 475
BslFI GGGAC 2 cut(s) 729, 1135
BsmAI GTCTC 1 cut(s) 1249
BsmFI GGGAC 2 cut(s) 729, 1135
BsnI GGCC 1 cut(s) 282
Bso31I GGTCTC 1 cut(s) 1249
Bsp1286I GDGCHC 1 cut(s) 1162
Bsp143I GATC 9 cut(s) 18, 96, 229, 334, 775, 781, 1177, 1273, 1392
Bsp19I CCATGG 2 cut(s) 439, 1259
BspANI GGCC 1 cut(s) 282
BspDI ATCGAT 1 cut(s) 95
BspHI TCATGA 1 cut(s) 1180
BspLI GGNNCC 3 cut(s) 7, 336, 864
BspMAI CTGCAG 1 cut(s) 591
BspOI GCTAGC 1 cut(s) 1409
BspPI GGATC 5 cut(s) 224, 329, 342, 776, 783
BspQI GCTCTTC 1 cut(s) 274
BspT107I GGYRCC 1 cut(s) 862
BspTNI GGTCTC 1 cut(s) 1249
BsrDI GCAATG 3 cut(s) 579, 1039, 1073
BsrI ACTGG 4 cut(s) 154, 418, 659, 926
BssECI CCNNGG 2 cut(s) 439, 1259
BssMI GATC 9 cut(s) 18, 96, 229, 334, 775, 781, 1177, 1273, 1392
BssT1I CCWWGG 2 cut(s) 439, 1259
Bst4CI ACNGT 3 cut(s) 798, 941, 1172
Bst6I CTCTTC 3 cut(s) 171, 274, 1239
BstC8I GCNNGC 4 cut(s) 48, 114, 1407, 1413
BstDEI CTNAG 2 cut(s) 946, 1139
BstDSI CCRYGG 2 cut(s) 439, 1259
BstEII GGTNACC 1 cut(s) 125
BstF5I GGATG 2 cut(s) 511, 685
BstHHI GCGC 2 cut(s) 511, 1411
BstKTI GATC 9 cut(s) 21, 99, 232, 337, 778, 784, 1180, 1276, 1395
BstMAI GTCTC 1 cut(s) 1249
BstMBI GATC 9 cut(s) 18, 96, 229, 334, 775, 781, 1177, 1273, 1392
BstMWI GCNNNNNNNGC 2 cut(s) 244, 807
BstNSI RCATGY 3 cut(s) 50, 976, 1415
BstPI GGTNACC 1 cut(s) 125
BstSCI CCNGG 1 cut(s) 473
BstSFI CTRYAG 2 cut(s) 587, 1168
BstV1I GCAGC 2 cut(s) 51, 196
BstX2I RGATCY 2 cut(s) 334, 775
BstXI CCANNNNNNTGG 1 cut(s) 366
BstYI RGATCY 2 cut(s) 334, 775
Bsu15I ATCGAT 1 cut(s) 95
BsuI GTATCC 2 cut(s) 635, 1280
BsuRI GGCC 1 cut(s) 282
BsuTUI ATCGAT 1 cut(s) 95
BtgI CCRYGG 2 cut(s) 439, 1259
BtgZI GCGATG 1 cut(s) 1093
BtsCI GGATG 2 cut(s) 511, 685
BtsIMutI CAGTG 1 cut(s) 1177
Cac8I GCNNGC 4 cut(s) 48, 114, 1407, 1413
CciI TCATGA 1 cut(s) 1180
CfoI GCGC 2 cut(s) 511, 1411
Cfr13I GGNCC 2 cut(s) 257, 299
ClaI ATCGAT 1 cut(s) 95
Csp6I GTAC 3 cut(s) 411, 968, 1206
CviQI GTAC 3 cut(s) 411, 968, 1206
DdeI CTNAG 2 cut(s) 946, 1139
DpnI GATC 9 cut(s) 20, 98, 231, 336, 777, 783, 1179, 1275, 1394
DpnII GATC 9 cut(s) 18, 96, 229, 334, 775, 781, 1177, 1273, 1392
DriI GACNNNNNGTC 1 cut(s) 255
Eam1104I CTCTTC 3 cut(s) 171, 274, 1239
Eam1105I GACNNNNNGTC 1 cut(s) 255
EarI CTCTTC 3 cut(s) 171, 274, 1239
Eco130I CCWWGG 2 cut(s) 439, 1259
Eco31I GGTCTC 1 cut(s) 1249
Eco32I GATATC 1 cut(s) 883
Eco47I GGWCC 2 cut(s) 257, 299
Eco91I GGTNACC 1 cut(s) 125
EcoO109I RGGNCCY 1 cut(s) 257
EcoO65I GGTNACC 1 cut(s) 125
EcoRV GATATC 1 cut(s) 883
EcoT14I CCWWGG 2 cut(s) 439, 1259
ErhI CCWWGG 2 cut(s) 439, 1259
FaqI GGGAC 2 cut(s) 729, 1135
FauNDI CATATG 1 cut(s) 1327
FbaI TGATCA 4 cut(s) 18, 1177, 1273, 1392
Fnu4HI GCNGC 2 cut(s) 65, 185
FokI GGATG 2 cut(s) 498, 692
Fsp4HI GCNGC 2 cut(s) 65, 185
FspBI CTAG 4 cut(s) 347, 453, 1211, 1406
GlaI GCGC 2 cut(s) 510, 1410
GluI GCNGC 2 cut(s) 65, 185
GsaI CCCAGC 1 cut(s) 716
GsuI CTGGAG 2 cut(s) 932, 1344
HaeIII GGCC 1 cut(s) 282
HapII CCGG 1 cut(s) 475
HhaI GCGC 2 cut(s) 511, 1411
Hin6I GCGC 2 cut(s) 509, 1409
HinP1I GCGC 2 cut(s) 509, 1409
HindIII AAGCTT 1 cut(s) 140
HpaII CCGG 1 cut(s) 475
HphI GGTGA 3 cut(s) 137, 560, 1187
Hpy166II GTNNAC 2 cut(s) 970, 1208
Hpy188I TCNGA 4 cut(s) 175, 211, 354, 727
Hpy188III TCNNGA 9 cut(s) 103, 221, 690, 779, 878, 911, 1049, 1181, 1361
Hpy8I GTNNAC 2 cut(s) 970, 1208
Hpy99I CGWCG 1 cut(s) 334
HpyAV CCTTC 3 cut(s) 821, 884, 1163
HpyCH4III ACNGT 3 cut(s) 798, 941, 1172
HpyCH4IV ACGT 2 cut(s) 27, 461
HpyCH4V TGCA 9 cut(s) 89, 184, 527, 589, 759, 831, 1080, 1283, 1415
HpyF10VI GCNNNNNNNGC 2 cut(s) 244, 807
HpyF3I CTNAG 2 cut(s) 946, 1139
HpySE526I ACGT 2 cut(s) 27, 461
HspAI GCGC 2 cut(s) 509, 1409
Ksp22I TGATCA 4 cut(s) 18, 1177, 1273, 1392
Kzo9I GATC 9 cut(s) 18, 96, 229, 334, 775, 781, 1177, 1273, 1392
LguI GCTCTTC 1 cut(s) 274
LmnI GCTCC 1 cut(s) 1363
Lsp1109I GCAGC 2 cut(s) 51, 196
LweI GCATC 3 cut(s) 254, 388, 520
MaeI CTAG 4 cut(s) 347, 453, 1211, 1406
MaeII ACGT 2 cut(s) 27, 461
MaeIII GTNAC 3 cut(s) 125, 416, 462
MalI GATC 9 cut(s) 20, 98, 231, 336, 777, 783, 1179, 1275, 1394
MboI GATC 9 cut(s) 18, 96, 229, 334, 775, 781, 1177, 1273, 1392
MboII GAAGA 4 cut(s) 188, 261, 995, 1256
MfeI CAATTG 2 cut(s) 819, 1239
MflI RGATCY 2 cut(s) 334, 775
MhlI GDGCHC 1 cut(s) 1162
MlyI GAGTC 3 cut(s) 215, 226, 935
MmeI TCCRAC 3 cut(s) 10, 36, 99
MseI TTAA 5 cut(s) 341, 446, 494, 684, 1041
MslI CAYNNNNRTG 1 cut(s) 1213
MspA1I CMGCKG 2 cut(s) 187, 712
MspI CCGG 1 cut(s) 475
MspR9I CCNGG 1 cut(s) 475
MunI CAATTG 2 cut(s) 819, 1239
MwoI GCNNNNNNNGC 2 cut(s) 244, 807
NciI CCSGG 1 cut(s) 475
NcoI CCATGG 2 cut(s) 439, 1259
NdeI CATATG 1 cut(s) 1327
NdeII GATC 9 cut(s) 18, 96, 229, 334, 775, 781, 1177, 1273, 1392
NheI GCTAGC 1 cut(s) 1405
NlaIV GGNNCC 3 cut(s) 7, 336, 864
NmuCI GTSAC 1 cut(s) 125
NspI RCATGY 3 cut(s) 50, 976, 1415
PaeI GCATGC 2 cut(s) 50, 1415
PagI TCATGA 1 cut(s) 1180
PciI ACATGT 1 cut(s) 972
PciSI GCTCTTC 1 cut(s) 274
PfeI GAWTC 7 cut(s) 33, 170, 355, 551, 615, 631, 1223
PkrI GCNGC 2 cut(s) 66, 186
PleI GAGTC 3 cut(s) 214, 225, 934
PpsI GAGTC 3 cut(s) 214, 225, 934
PpuMI RGGWCCY 1 cut(s) 257
PscI ACATGT 1 cut(s) 972
Psp5II RGGWCCY 1 cut(s) 257
PspEI GGTNACC 1 cut(s) 125
PspFI CCCAGC 1 cut(s) 712
PspN4I GGNNCC 3 cut(s) 7, 336, 864
PspPI GGNCC 2 cut(s) 257, 299
PspPPI RGGWCCY 1 cut(s) 257
PstI CTGCAG 1 cut(s) 591
PsuI RGATCY 2 cut(s) 334, 775
PvuII CAGCTG 2 cut(s) 187, 712
RsaI GTAC 3 cut(s) 412, 969, 1207
RsaNI GTAC 3 cut(s) 411, 968, 1206
RseI CAYNNNNRTG 1 cut(s) 1213
SapI GCTCTTC 1 cut(s) 274
SaqAI TTAA 5 cut(s) 341, 446, 494, 684, 1041
SatI GCNGC 2 cut(s) 65, 185
Sau3AI GATC 9 cut(s) 18, 96, 229, 334, 775, 781, 1177, 1273, 1392
Sau96I GGNCC 2 cut(s) 257, 299
SchI GAGTC 3 cut(s) 215, 226, 935
ScrFI CCNGG 1 cut(s) 475
SduI GDGCHC 1 cut(s) 1162
SfaNI GCATC 3 cut(s) 254, 388, 520
SfcI CTRYAG 2 cut(s) 587, 1168
SinI GGWCC 2 cut(s) 257, 299
SmiMI CAYNNNNRTG 1 cut(s) 1213
SmlI CTYRAG 1 cut(s) 635
SmoI CTYRAG 1 cut(s) 635
SpeI ACTAGT 1 cut(s) 346
SphI GCATGC 2 cut(s) 50, 1415
SspI AATATT 2 cut(s) 390, 1120
SspMI CTAG 4 cut(s) 347, 453, 1211, 1406
StyD4I CCNGG 1 cut(s) 473
StyI CCWWGG 2 cut(s) 439, 1259
TaaI ACNGT 3 cut(s) 798, 941, 1172
TaiI ACGT 2 cut(s) 30, 464
TaqI TCGA 5 cut(s) 95, 262, 329, 780, 1132
TatI WGTACW 1 cut(s) 1205
TfiI GAWTC 7 cut(s) 33, 170, 355, 551, 615, 631, 1223
Tru1I TTAA 5 cut(s) 341, 446, 494, 684, 1041
Tru9I TTAA 5 cut(s) 341, 446, 494, 684, 1041
TscAI CASTG 1 cut(s) 1177
TseFI GTSAC 1 cut(s) 125
TseI GCWGC 2 cut(s) 64, 184
Tsp45I GTSAC 1 cut(s) 125
TspDTI ATGAA 6 cut(s) 110, 306, 864, 932, 1037, 1244
TspGWI ACGGA 1 cut(s) 347
TspRI CASTG 1 cut(s) 1177
VpaK11BI GGWCC 2 cut(s) 257, 299
XapI RAATTY 6 cut(s) 322, 529, 625, 767, 901, 990
XceI RCATGY 3 cut(s) 50, 976, 1415
XspI CTAG 4 cut(s) 347, 453, 1211, 1406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.