Rh6AG132800

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0004218
Physical Location & Seq
Forward (+)
20103901 .. 20108110
4210 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG132800.1

Sequence Viewer

Length: 1425 bp
ATGCGATCCAACATGATCATCATTTACGTTAGATTCTGGCTGTGGCATGCTTTTATTCTACTGCTGCTTACTTCCAACTTTGCTCTTGCACAGTCGATCATCAAGAACTTACCAGCCTTCCAGGGTGACCTCCCTTTCAAGCTTGAAACTGGGTATGTGGGCGTGGGAGATTCAGAAGAGGTGCAACTGTTCTACTACTTTGTTGAGTCAGAAAGGAGTCCAGAAAATGATCCTCTGCTGATTTGGTTGACAGGAGGTCCTCGATGCTCTTCGTTTTATGGCCTCATCTACGAAATTGGTCCATTATCTTTCAAACTTTCAAATTTGACTACAGATCCCTTAACACTTGTTCTGAATCCAAACTCGTGGAACAAGGTAGCAAACATCATATTCTTAGATGCTCCTGCTGGTACTGGTTACTCCTACTCCACCACTTCCCACGGGTTAATCTCTAGTGACACCTTACATTCAACCCGTATTTGTGAGTTTCTCCAGAAGTGGCTTACGACTCATCAAAAGTTCCTTGCAAATCCACTCTATGTTGCTGGTGACTCTTACTCTGGCAAAATCGTTCCCATCATTGCCCAGAAGATAGCTGATGGTATTGAAGCTGGAATCAAGCCACATTTGAATCTCCAGGGATACATAGTTGGTAATCCAGTTTCAAATGCACAACAGGACGTTAATTCAAGAATTGTATATGCTCAGCTTCTGGGACTTATATCAGATAGAATATATCAGTCAGCTAACAGGAATTGCAAGGGCGAATTTGTAGATTTTGATCCAAACAATCAACTGTGCCTGAACAGCCTTCAAGCAATCGATGAGTGCATTGGGAGAATTGATAAAGAACATATCTTGGCACCATTTTGCCTTGCATCAATTAACCATAACCAAAATTCCATACTGGAAATGAACTGGGAGTCTAATGATGACAGTTTCTTGGACTTTTCATTTCCTATGGTACAAATGTGTAGGGAAGATAAGATTCGGTATTCCGTTCTATGGCAAAATGATATAAATGTTCGCAAAGCTCTTGGTATTCGGGAGGGAACAAAAGGGCAATGGACTAAATGCAATAAAACCACTCCCTATACATTTGACATCGCAAGTAATATTGCGTATCATCGCAATCTCAGCCGAAAATCCCTTCGTGCTATAATCTACAGCGGTGATCATGACATGTCTGTTCCATATATAAGTACACTAGCATGGATAGAATCCATGAACTTTACAATTGAAGAGGATTGGAGACCATGGTTCTCTAATGATCAAGTTGCAGGATACACTCTTTACTATTCGAATAATAAGGAGTATAATTTGACCTATGCGACAATAAAGGGAGGAGGACATACAGCTCCAGAATACAATCCCAAGGAATGTTTTGATATGATCAGTAGGTGGCTAGCGCATACGCCTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

474

Amino Acids

53.9

Weight (kDa)

5.69

Isoelectric Point (pI)

41.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 38 - 470 8.7e-121 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 862
AciI CCGC 1 cut(s) 1170
AclWI GGATC 3 cut(s) 224, 329, 776
AcsI RAATTY 3 cut(s) 322, 767, 898
AfaI GTAC 3 cut(s) 412, 966, 1204
AfiI CCNNNNNNNGG 1 cut(s) 1005
AflIII ACRYGT 1 cut(s) 1182
AhdI GACNNNNNGTC 1 cut(s) 255
AjnI CCWGG 2 cut(s) 120, 636
AluBI AGCT 7 cut(s) 142, 596, 611, 709, 746, 1034, 1358
AluI AGCT 7 cut(s) 142, 596, 611, 709, 746, 1034, 1358
Alw26I GTCTC 1 cut(s) 1246
AlwI GGATC 3 cut(s) 224, 329, 776
AlwNI CAGNNNCTG 1 cut(s) 712
AoxI GGCC 1 cut(s) 280
ApeKI GCWGC 1 cut(s) 64
ApoI RAATTY 3 cut(s) 322, 767, 898
AspLEI GCGC 1 cut(s) 1411
AspS9I GGNCC 2 cut(s) 257, 299
AsuHPI GGTGA 3 cut(s) 137, 560, 1184
AsuII TTCGAA 1 cut(s) 1301
AsuNHI GCTAGC 1 cut(s) 1405
AvaII GGWCC 2 cut(s) 257, 299
BaeI ACNNNNGTAYC 2 cut(s) 956, 989
BanI GGYRCC 1 cut(s) 862
BauI CACGAG 1 cut(s) 364
BbvI GCAGC 1 cut(s) 51
BccI CCATC 2 cut(s) 584, 593
BciT130I CCWGG 2 cut(s) 122, 638
BciVI GTATCC 2 cut(s) 635, 1277
BclI TGATCA 4 cut(s) 15, 1174, 1270, 1392
BcoDI GTCTC 1 cut(s) 1246
BfaI CTAG 3 cut(s) 453, 1208, 1406
BfmI CTRYAG 2 cut(s) 330, 1165
BfuI GTATCC 2 cut(s) 635, 1277
BisI GCNGC 1 cut(s) 65
BlpI GCTNAGC 1 cut(s) 705
BlsI GCNGC 1 cut(s) 66
Bme1390I CCNGG 2 cut(s) 122, 638
Bme18I GGWCC 2 cut(s) 257, 299
BmeRI GACNNNNNGTC 1 cut(s) 255
BmgT120I GGNCC 2 cut(s) 257, 299
BmiI GGNNCC 1 cut(s) 864
BmrFI CCNGG 2 cut(s) 122, 638
BmrI ACTGGG 2 cut(s) 159, 928
BmsI GCATC 3 cut(s) 254, 388, 887
BmtI GCTAGC 1 cut(s) 1409
BmuI ACTGGG 2 cut(s) 159, 928
BpmI CTGGAG 3 cut(s) 476, 620, 1344
Bpu1102I GCTNAGC 1 cut(s) 705
Bpu14I TTCGAA 1 cut(s) 1301
Bsa29I ATCGAT 1 cut(s) 822
BsaBI GATNNNNATC 1 cut(s) 780
BsaI GGTCTC 1 cut(s) 1246
BsaJI CCNNGG 5 cut(s) 121, 439, 637, 1256, 1374
BsaXI ACNNNNNCTCC 2 cut(s) 410, 440
Bsc4I CCNNNNNNNGG 1 cut(s) 1005
Bse1I ACTGG 5 cut(s) 154, 418, 659, 912, 923
Bse3DI GCAATG 2 cut(s) 579, 1070
Bse8I GATNNNNATC 1 cut(s) 780
BseBI CCWGG 2 cut(s) 122, 638
BseCI ATCGAT 1 cut(s) 822
BseDI CCNNGG 5 cut(s) 121, 439, 637, 1256, 1374
BseJI GATNNNNATC 1 cut(s) 780
BseLI CCNNNNNNNGG 1 cut(s) 1005
BseMI GCAATG 2 cut(s) 579, 1070
BseMII CTCAG 2 cut(s) 719, 1150
BseNI ACTGG 5 cut(s) 154, 418, 659, 912, 923
BseRI GAGGAG 1 cut(s) 1359
BseXI GCAGC 1 cut(s) 51
BshFI GGCC 1 cut(s) 282
BshNI GGYRCC 1 cut(s) 862
BshVI ATCGAT 1 cut(s) 822
BslFI GGGAC 1 cut(s) 729
BslI CCNNNNNNNGG 1 cut(s) 1005
BsmAI GTCTC 1 cut(s) 1246
BsmFI GGGAC 1 cut(s) 729
BsnI GGCC 1 cut(s) 282
Bso31I GGTCTC 1 cut(s) 1246
Bsp119I TTCGAA 1 cut(s) 1301
Bsp143I GATC 9 cut(s) 5, 15, 96, 229, 334, 781, 1174, 1270, 1392
Bsp1720I GCTNAGC 1 cut(s) 705
Bsp19I CCATGG 1 cut(s) 1256
BspACI CCGC 1 cut(s) 1170
BspANI GGCC 1 cut(s) 282
BspCNI CTCAG 2 cut(s) 718, 1149
BspDI ATCGAT 1 cut(s) 822
BspHI TCATGA 1 cut(s) 1177
BspLI GGNNCC 1 cut(s) 864
BspOI GCTAGC 1 cut(s) 1409
BspPI GGATC 3 cut(s) 224, 329, 776
BspQI GCTCTTC 1 cut(s) 274
BspT104I TTCGAA 1 cut(s) 1301
BspT107I GGYRCC 1 cut(s) 862
BspTNI GGTCTC 1 cut(s) 1246
BsrDI GCAATG 2 cut(s) 579, 1070
BsrI ACTGG 5 cut(s) 154, 418, 659, 912, 923
BssECI CCNNGG 5 cut(s) 121, 439, 637, 1256, 1374
BssMI GATC 9 cut(s) 5, 15, 96, 229, 334, 781, 1174, 1270, 1392
BssSI CACGAG 1 cut(s) 364
BssT1I CCWWGG 2 cut(s) 1256, 1374
Bst2BI CACGAG 1 cut(s) 364
Bst2UI CCWGG 2 cut(s) 122, 638
Bst4CI ACNGT 4 cut(s) 93, 189, 798, 938
Bst6I CTCTTC 3 cut(s) 171, 274, 1236
BstBI TTCGAA 1 cut(s) 1301
BstC8I GCNNGC 2 cut(s) 48, 1407
BstDEI CTNAG 3 cut(s) 394, 705, 1136
BstDSI CCRYGG 2 cut(s) 439, 1256
BstEII GGTNACC 1 cut(s) 125
BstHHI GCGC 1 cut(s) 1411
BstKTI GATC 9 cut(s) 8, 18, 99, 232, 337, 784, 1177, 1273, 1395
BstMAI GTCTC 1 cut(s) 1246
BstMBI GATC 9 cut(s) 5, 15, 96, 229, 334, 781, 1174, 1270, 1392
BstMWI GCNNNNNNNGC 2 cut(s) 807, 1137
BstNI CCWGG 2 cut(s) 122, 638
BstNSI RCATGY 2 cut(s) 50, 1186
BstPI GGTNACC 1 cut(s) 125
BstSCI CCNGG 2 cut(s) 120, 636
BstSFI CTRYAG 2 cut(s) 330, 1165
BstV1I GCAGC 1 cut(s) 51
BstX2I RGATCY 1 cut(s) 334
BstXI CCANNNNNNTGG 1 cut(s) 366
BstYI RGATCY 1 cut(s) 334
Bsu15I ATCGAT 1 cut(s) 822
BsuI GTATCC 2 cut(s) 635, 1277
BsuRI GGCC 1 cut(s) 282
BsuTUI ATCGAT 1 cut(s) 822
BtgI CCRYGG 2 cut(s) 439, 1256
BtgZI GCGATG 2 cut(s) 1090, 1112
Cac8I GCNNGC 2 cut(s) 48, 1407
CaiI CAGNNNCTG 1 cut(s) 712
CciI TCATGA 1 cut(s) 1177
CfoI GCGC 1 cut(s) 1411
Cfr13I GGNCC 2 cut(s) 257, 299
ClaI ATCGAT 1 cut(s) 822
Csp6I GTAC 3 cut(s) 411, 965, 1203
CviAII CATG 7 cut(s) 13, 47, 1178, 1183, 1212, 1225, 1257
CviQI GTAC 3 cut(s) 411, 965, 1203
DdeI CTNAG 3 cut(s) 394, 705, 1136
DpnI GATC 9 cut(s) 7, 17, 98, 231, 336, 783, 1176, 1272, 1394
DpnII GATC 9 cut(s) 5, 15, 96, 229, 334, 781, 1174, 1270, 1392
DriI GACNNNNNGTC 1 cut(s) 255
Eam1104I CTCTTC 3 cut(s) 171, 274, 1236
Eam1105I GACNNNNNGTC 1 cut(s) 255
EarI CTCTTC 3 cut(s) 171, 274, 1236
Eco130I CCWWGG 2 cut(s) 1256, 1374
Eco31I GGTCTC 1 cut(s) 1246
Eco47I GGWCC 2 cut(s) 257, 299
Eco91I GGTNACC 1 cut(s) 125
EcoO109I RGGNCCY 1 cut(s) 257
EcoO65I GGTNACC 1 cut(s) 125
EcoRII CCWGG 2 cut(s) 120, 636
EcoT14I CCWWGG 2 cut(s) 1256, 1374
ErhI CCWWGG 2 cut(s) 1256, 1374
FaeI CATG 7 cut(s) 16, 50, 1181, 1186, 1215, 1228, 1260
FaqI GGGAC 1 cut(s) 729
FatI CATG 7 cut(s) 12, 46, 1177, 1182, 1211, 1224, 1256
FbaI TGATCA 4 cut(s) 15, 1174, 1270, 1392
Fnu4HI GCNGC 1 cut(s) 65
Fsp4HI GCNGC 1 cut(s) 65
FspBI CTAG 3 cut(s) 453, 1208, 1406
GlaI GCGC 1 cut(s) 1410
GluI GCNGC 1 cut(s) 65
GsuI CTGGAG 3 cut(s) 476, 620, 1344
HaeIII GGCC 1 cut(s) 282
HhaI GCGC 1 cut(s) 1411
Hin1II CATG 7 cut(s) 16, 50, 1181, 1186, 1215, 1228, 1260
Hin6I GCGC 1 cut(s) 1409
HinP1I GCGC 1 cut(s) 1409
HincII GTYRAC 1 cut(s) 249
HindII GTYRAC 1 cut(s) 249
HindIII AAGCTT 1 cut(s) 140
HphI GGTGA 3 cut(s) 137, 560, 1184
Hpy166II GTNNAC 2 cut(s) 249, 1205
Hpy188I TCNGA 4 cut(s) 175, 211, 354, 727
Hpy188III TCNNGA 7 cut(s) 103, 221, 493, 690, 1046, 1178, 1361
Hpy8I GTNNAC 2 cut(s) 249, 1205
HpyAV CCTTC 3 cut(s) 127, 821, 1160
HpyCH4III ACNGT 4 cut(s) 93, 189, 798, 938
HpyCH4IV ACGT 2 cut(s) 27, 681
HpyCH4V TGCA 9 cut(s) 89, 184, 527, 671, 759, 831, 878, 1077, 1280
HpyF10VI GCNNNNNNNGC 2 cut(s) 807, 1137
HpyF3I CTNAG 3 cut(s) 394, 705, 1136
HpySE526I ACGT 2 cut(s) 27, 681
Hsp92II CATG 7 cut(s) 16, 50, 1181, 1186, 1215, 1228, 1260
HspAI GCGC 1 cut(s) 1409
Ksp22I TGATCA 4 cut(s) 15, 1174, 1270, 1392
Kzo9I GATC 9 cut(s) 5, 15, 96, 229, 334, 781, 1174, 1270, 1392
LguI GCTCTTC 1 cut(s) 274
LmnI GCTCC 2 cut(s) 406, 1363
Lsp1109I GCAGC 1 cut(s) 51
LweI GCATC 3 cut(s) 254, 388, 887
MaeI CTAG 3 cut(s) 453, 1208, 1406
MaeII ACGT 2 cut(s) 27, 681
MaeIII GTNAC 4 cut(s) 125, 416, 455, 548
MalI GATC 9 cut(s) 7, 17, 98, 231, 336, 783, 1176, 1272, 1394
MboI GATC 9 cut(s) 5, 15, 96, 229, 334, 781, 1174, 1270, 1392
MboII GAAGA 5 cut(s) 188, 261, 601, 992, 1253
MfeI CAATTG 1 cut(s) 1236
MflI RGATCY 1 cut(s) 334
MlyI GAGTC 5 cut(s) 215, 226, 502, 545, 932
MmeI TCCRAC 2 cut(s) 33, 99
MseI TTAA 4 cut(s) 341, 446, 684, 885
MslI CAYNNNNRTG 1 cut(s) 1210
MspA1I CMGCKG 1 cut(s) 1170
MspR9I CCNGG 2 cut(s) 122, 638
MunI CAATTG 1 cut(s) 1236
MvaI CCWGG 2 cut(s) 122, 638
MwoI GCNNNNNNNGC 2 cut(s) 807, 1137
NcoI CCATGG 1 cut(s) 1256
NdeII GATC 9 cut(s) 5, 15, 96, 229, 334, 781, 1174, 1270, 1392
NheI GCTAGC 1 cut(s) 1405
NlaIII CATG 7 cut(s) 16, 50, 1181, 1186, 1215, 1228, 1260
NlaIV GGNNCC 1 cut(s) 864
NmuCI GTSAC 3 cut(s) 125, 455, 548
NspI RCATGY 2 cut(s) 50, 1186
NspV TTCGAA 1 cut(s) 1301
PaeI GCATGC 1 cut(s) 50
PagI TCATGA 1 cut(s) 1177
PciI ACATGT 1 cut(s) 1182
PciSI GCTCTTC 1 cut(s) 274
PfeI GAWTC 7 cut(s) 33, 170, 355, 615, 631, 988, 1220
PkrI GCNGC 1 cut(s) 66
PleI GAGTC 5 cut(s) 214, 225, 502, 545, 931
PpsI GAGTC 5 cut(s) 214, 225, 502, 545, 931
PpuMI RGGWCCY 1 cut(s) 257
PscI ACATGT 1 cut(s) 1182
Psp5II RGGWCCY 1 cut(s) 257
Psp6I CCWGG 2 cut(s) 120, 636
PspEI GGTNACC 1 cut(s) 125
PspGI CCWGG 2 cut(s) 120, 636
PspN4I GGNNCC 1 cut(s) 864
PspPI GGNCC 2 cut(s) 257, 299
PspPPI RGGWCCY 1 cut(s) 257
PstNI CAGNNNCTG 1 cut(s) 712
PsuI RGATCY 1 cut(s) 334
RsaI GTAC 3 cut(s) 412, 966, 1204
RsaNI GTAC 3 cut(s) 411, 965, 1203
RseI CAYNNNNRTG 1 cut(s) 1210
SapI GCTCTTC 1 cut(s) 274
SaqAI TTAA 4 cut(s) 341, 446, 684, 885
SatI GCNGC 1 cut(s) 65
Sau3AI GATC 9 cut(s) 5, 15, 96, 229, 334, 781, 1174, 1270, 1392
Sau96I GGNCC 2 cut(s) 257, 299
SchI GAGTC 5 cut(s) 215, 226, 502, 545, 932
ScrFI CCNGG 2 cut(s) 122, 638
SfaNI GCATC 3 cut(s) 254, 388, 887
SfcI CTRYAG 2 cut(s) 330, 1165
SfuI TTCGAA 1 cut(s) 1301
SinI GGWCC 2 cut(s) 257, 299
SmiMI CAYNNNNRTG 1 cut(s) 1210
SphI GCATGC 1 cut(s) 50
SsiI CCGC 1 cut(s) 1170
SspI AATATT 1 cut(s) 1117
SspMI CTAG 3 cut(s) 453, 1208, 1406
StyD4I CCNGG 2 cut(s) 120, 636
StyI CCWWGG 2 cut(s) 1256, 1374
TaaI ACNGT 4 cut(s) 93, 189, 798, 938
TaiI ACGT 2 cut(s) 30, 684
TaqI TCGA 4 cut(s) 95, 262, 822, 1301
TatI WGTACW 1 cut(s) 1202
TfiI GAWTC 7 cut(s) 33, 170, 355, 615, 631, 988, 1220
Tru1I TTAA 4 cut(s) 341, 446, 684, 885
Tru9I TTAA 4 cut(s) 341, 446, 684, 885
TseFI GTSAC 3 cut(s) 125, 455, 548
TseI GCWGC 1 cut(s) 64
Tsp45I GTSAC 3 cut(s) 125, 455, 548
TspDTI ATGAA 3 cut(s) 929, 942, 1241
TspGWI ACGGA 1 cut(s) 988
VpaK11BI GGWCC 2 cut(s) 257, 299
XapI RAATTY 3 cut(s) 322, 767, 898
XceI RCATGY 2 cut(s) 50, 1186
XspI CTAG 3 cut(s) 453, 1208, 1406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.