Rroxscaffold_176G00431840

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000176
OG0004218
Physical Location & Seq
Forward (+)
1720849 .. 1725071
4223 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_176G00431840.1

Sequence Viewer

Length: 1329 bp
ATGCGATCCAACGTGATCATTTACGTTAGATTCTGGCTGTGGCATGCTTTTATTCTACTGCTGCTTACTTCCAACTTTGCTCTTGCACAGTCTATCATCAAGAACTTACCAGCCTTCCAGGGTGACCTCCCTTTCAAGCTTGAAACTGGGTATGTGGGCGTGGGAGATTCAGAAGACGTGCAACTGTTCTACTACTTTGTTGAGTCAGAAAGGAGTCCAGAAAATGATCCTCTGCTGATTTGGTTGACTGGAGGTCCTCGATGCTCTTCGTTTTATGGCCTCATCTACGAAATTGGTCCATTATCTTTCAAATTTACAAATTTGACTACAGATCCCTTAACACTTGTTCTGAATCCAAACTCATGGAACAAGGTAGCAAACATCATATTCTTAGATGCTCCTGCTGGTACTGGTTACTCCTACTCCACCACTTCCCACGGGTTAATCTCTAGTGACACCTTACATTCAACCCGTATTTATAAGTTTCTCCAGAAGTGGCTTACGACTCATCAAAAGTTCCTTGCAAATCCACTCTATGTTGCTGGTGACTCTTACTCTGGCAAAGTCGTTCCCATCATTGCCCAGAAGATAGCTGATGGTATTGAAGCTGGAATCAAGCCACAATTGAATCTCAAAGGATACATAGTTGGCAATCCAGTTTCAAATGGACAACAGGACGTTAATTCCAGAATTGTATATGCTCATCAGCTGGGACTTATATCGGATAGAATATATCAGTCAGCTAACAGGAATTGCAAGGGTGAATTTGTAGATTTTGATCCAAACAATCAACTGTGCCTGAACAGCCTTCAAGCAATCGATGAGTGCATTGGGAGAATTGATAAAGAACATATCTTGGCACCATTTTGCCTTGCATCAATTAACCATAACCAAAATTCCATACTGGAAATGAACTGGGAGTCTAGTGATGACAGTTTCTTGGACTACTCATTTCCTATGGAAGATAAGATTCGGTATTCCATTCTATGGCAAAATGATATAAATGTTCGCAAAGCTCTTAGTATTCGGGAGGGAACAAAAGGGCAATGGGCTAAATGCAATAAAACCACTCCCTATACATTTGACATCGCAAGTAATATTGAGTATCATCGCAATCTGAGCCGAAAATCCCTTCGTGCTATAATCTACAGCGGTGATCATGACATGAGTGTTCCATATATAAGTACACTAGCATGGATAGAATCCATGAACTTTACAATTGAAGAGGATTGGAGACCATGGTTCTCTAATGATCAAGTTGCAGGATACACTCTTTACTATTCGAATAATAAAGAGTATAATTTGACCTATGCGACAATAAAGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

442

Amino Acids

50.34

Weight (kDa)

5.75

Isoelectric Point (pI)

40.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 37 - 441 1.5e-110 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 480
AccB1I GGYRCC 1 cut(s) 859
AccB7I CCANNNNNTGG 1 cut(s) 987
AciI CCGC 1 cut(s) 1152
AclWI GGATC 3 cut(s) 221, 326, 773
AcsI RAATTY 4 cut(s) 311, 319, 764, 895
AfaI GTAC 2 cut(s) 409, 1186
AfiI CCNNNNNNNGG 1 cut(s) 987
AgsI TTSAA 9 cut(s) 136, 143, 310, 468, 605, 628, 663, 812, 1223
AhdI GACNNNNNGTC 1 cut(s) 252
AjiI CACGTC 1 cut(s) 178
AjnI CCWGG 1 cut(s) 117
AluBI AGCT 6 cut(s) 139, 593, 608, 709, 743, 1016
AluI AGCT 6 cut(s) 139, 593, 608, 709, 743, 1016
Alw26I GTCTC 1 cut(s) 1228
AlwI GGATC 3 cut(s) 221, 326, 773
AoxI GGCC 1 cut(s) 277
ApeKI GCWGC 1 cut(s) 61
ApoI RAATTY 4 cut(s) 311, 319, 764, 895
AspS9I GGNCC 2 cut(s) 254, 296
AsuHPI GGTGA 4 cut(s) 134, 557, 773, 1166
AsuII TTCGAA 1 cut(s) 1283
AvaII GGWCC 2 cut(s) 254, 296
BanI GGYRCC 1 cut(s) 859
BbsI GAAGAC 1 cut(s) 180
BbvI GCAGC 1 cut(s) 48
BccI CCATC 2 cut(s) 581, 590
BciT130I CCWGG 1 cut(s) 119
BciVI GTATCC 2 cut(s) 632, 1259
BclI TGATCA 3 cut(s) 15, 1156, 1252
BcoDI GTCTC 1 cut(s) 1228
BfaI CTAG 3 cut(s) 450, 924, 1190
BfmI CTRYAG 2 cut(s) 327, 1147
BfuI GTATCC 2 cut(s) 632, 1259
BisI GCNGC 1 cut(s) 62
BlsI GCNGC 1 cut(s) 63
Bme1390I CCNGG 1 cut(s) 119
Bme18I GGWCC 2 cut(s) 254, 296
BmeRI GACNNNNNGTC 1 cut(s) 252
BmgBI CACGTC 1 cut(s) 178
BmgT120I GGNCC 2 cut(s) 254, 296
BmiI GGNNCC 1 cut(s) 861
BmrFI CCNGG 1 cut(s) 119
BmrI ACTGGG 2 cut(s) 156, 925
BmsI GCATC 3 cut(s) 251, 385, 884
BmuI ACTGGG 2 cut(s) 156, 925
BpiI GAAGAC 1 cut(s) 180
BpmI CTGGAG 2 cut(s) 270, 473
Bpu14I TTCGAA 1 cut(s) 1283
Bsa29I ATCGAT 1 cut(s) 819
BsaBI GATNNNNATC 1 cut(s) 777
BsaI GGTCTC 1 cut(s) 1228
BsaJI CCNNGG 3 cut(s) 118, 436, 1238
BsaXI ACNNNNNCTCC 2 cut(s) 407, 437
Bsc4I CCNNNNNNNGG 1 cut(s) 987
Bse1I ACTGG 6 cut(s) 151, 253, 415, 656, 909, 920
Bse3DI GCAATG 2 cut(s) 576, 1052
Bse8I GATNNNNATC 1 cut(s) 777
BseBI CCWGG 1 cut(s) 119
BseCI ATCGAT 1 cut(s) 819
BseDI CCNNGG 3 cut(s) 118, 436, 1238
BseJI GATNNNNATC 1 cut(s) 777
BseLI CCNNNNNNNGG 1 cut(s) 987
BseMI GCAATG 2 cut(s) 576, 1052
BseMII CTCAG 1 cut(s) 1109
BseNI ACTGG 6 cut(s) 151, 253, 415, 656, 909, 920
BseXI GCAGC 1 cut(s) 48
BseYI CCCAGC 1 cut(s) 709
BshFI GGCC 1 cut(s) 279
BshNI GGYRCC 1 cut(s) 859
BshVI ATCGAT 1 cut(s) 819
BslFI GGGAC 1 cut(s) 726
BslI CCNNNNNNNGG 1 cut(s) 987
BsmAI GTCTC 1 cut(s) 1228
BsmFI GGGAC 1 cut(s) 726
BsnI GGCC 1 cut(s) 279
Bso31I GGTCTC 1 cut(s) 1228
Bsp119I TTCGAA 1 cut(s) 1283
Bsp143I GATC 7 cut(s) 5, 15, 226, 331, 778, 1156, 1252
Bsp19I CCATGG 1 cut(s) 1238
BspACI CCGC 1 cut(s) 1152
BspANI GGCC 1 cut(s) 279
BspCNI CTCAG 1 cut(s) 1110
BspDI ATCGAT 1 cut(s) 819
BspHI TCATGA 1 cut(s) 1159
BspLI GGNNCC 1 cut(s) 861
BspPI GGATC 3 cut(s) 221, 326, 773
BspQI GCTCTTC 1 cut(s) 271
BspT104I TTCGAA 1 cut(s) 1283
BspT107I GGYRCC 1 cut(s) 859
BspTNI GGTCTC 1 cut(s) 1228
BsrDI GCAATG 2 cut(s) 576, 1052
BsrI ACTGG 6 cut(s) 151, 253, 415, 656, 909, 920
BssECI CCNNGG 3 cut(s) 118, 436, 1238
BssMI GATC 7 cut(s) 5, 15, 226, 331, 778, 1156, 1252
BssT1I CCWWGG 1 cut(s) 1238
Bst2UI CCWGG 1 cut(s) 119
Bst4CI ACNGT 4 cut(s) 90, 186, 795, 935
Bst6I CTCTTC 2 cut(s) 271, 1218
BstBI TTCGAA 1 cut(s) 1283
BstC8I GCNNGC 1 cut(s) 45
BstDEI CTNAG 3 cut(s) 391, 1019, 1118
BstDSI CCRYGG 2 cut(s) 436, 1238
BstEII GGTNACC 1 cut(s) 122
BstKTI GATC 7 cut(s) 8, 18, 229, 334, 781, 1159, 1255
BstMAI GTCTC 1 cut(s) 1228
BstMBI GATC 7 cut(s) 5, 15, 226, 331, 778, 1156, 1252
BstMWI GCNNNNNNNGC 2 cut(s) 804, 1119
BstNI CCWGG 1 cut(s) 119
BstNSI RCATGY 1 cut(s) 47
BstPI GGTNACC 1 cut(s) 122
BstSCI CCNGG 1 cut(s) 117
BstSFI CTRYAG 2 cut(s) 327, 1147
BstV1I GCAGC 1 cut(s) 48
BstV2I GAAGAC 1 cut(s) 180
BstX2I RGATCY 1 cut(s) 331
BstXI CCANNNNNNTGG 1 cut(s) 363
BstYI RGATCY 1 cut(s) 331
Bsu15I ATCGAT 1 cut(s) 819
BsuI GTATCC 2 cut(s) 632, 1259
BsuRI GGCC 1 cut(s) 279
BsuTUI ATCGAT 1 cut(s) 819
BtgI CCRYGG 2 cut(s) 436, 1238
BtgZI GCGATG 2 cut(s) 1072, 1094
BtrI CACGTC 1 cut(s) 178
Cac8I GCNNGC 1 cut(s) 45
CciI TCATGA 1 cut(s) 1159
Cfr13I GGNCC 2 cut(s) 254, 296
ClaI ATCGAT 1 cut(s) 819
Csp6I GTAC 2 cut(s) 408, 1185
CviAII CATG 8 cut(s) 44, 363, 1160, 1165, 1194, 1207, 1239, 1326
CviQI GTAC 2 cut(s) 408, 1185
DdeI CTNAG 3 cut(s) 391, 1019, 1118
DpnI GATC 7 cut(s) 7, 17, 228, 333, 780, 1158, 1254
DpnII GATC 7 cut(s) 5, 15, 226, 331, 778, 1156, 1252
DriI GACNNNNNGTC 1 cut(s) 252
Eam1104I CTCTTC 2 cut(s) 271, 1218
Eam1105I GACNNNNNGTC 1 cut(s) 252
EarI CTCTTC 2 cut(s) 271, 1218
Eco130I CCWWGG 1 cut(s) 1238
Eco31I GGTCTC 1 cut(s) 1228
Eco47I GGWCC 2 cut(s) 254, 296
Eco91I GGTNACC 1 cut(s) 122
EcoO109I RGGNCCY 1 cut(s) 254
EcoO65I GGTNACC 1 cut(s) 122
EcoRII CCWGG 1 cut(s) 117
EcoT14I CCWWGG 1 cut(s) 1238
ErhI CCWWGG 1 cut(s) 1238
FaeI CATG 8 cut(s) 47, 366, 1163, 1168, 1197, 1210, 1242, 1329
FaqI GGGAC 1 cut(s) 726
FatI CATG 8 cut(s) 43, 362, 1159, 1164, 1193, 1206, 1238, 1325
FbaI TGATCA 3 cut(s) 15, 1156, 1252
Fnu4HI GCNGC 1 cut(s) 62
Fsp4HI GCNGC 1 cut(s) 62
FspBI CTAG 3 cut(s) 450, 924, 1190
GluI GCNGC 1 cut(s) 62
GsaI CCCAGC 1 cut(s) 713
GsuI CTGGAG 2 cut(s) 270, 473
HaeIII GGCC 1 cut(s) 279
Hin1II CATG 8 cut(s) 47, 366, 1163, 1168, 1197, 1210, 1242, 1329
HincII GTYRAC 1 cut(s) 246
HindII GTYRAC 1 cut(s) 246
HindIII AAGCTT 1 cut(s) 137
HphI GGTGA 4 cut(s) 134, 557, 773, 1166
Hpy166II GTNNAC 2 cut(s) 246, 1187
Hpy188I TCNGA 5 cut(s) 172, 208, 351, 724, 1119
Hpy188III TCNNGA 6 cut(s) 100, 218, 490, 687, 1028, 1160
Hpy8I GTNNAC 2 cut(s) 246, 1187
HpyAV CCTTC 3 cut(s) 124, 818, 1142
HpyCH4III ACNGT 4 cut(s) 90, 186, 795, 935
HpyCH4IV ACGT 4 cut(s) 12, 24, 177, 678
HpyCH4V TGCA 8 cut(s) 86, 181, 524, 756, 828, 875, 1059, 1262
HpyF10VI GCNNNNNNNGC 2 cut(s) 804, 1119
HpyF3I CTNAG 3 cut(s) 391, 1019, 1118
HpySE526I ACGT 4 cut(s) 12, 24, 177, 678
Hsp92II CATG 8 cut(s) 47, 366, 1163, 1168, 1197, 1210, 1242, 1329
Ksp22I TGATCA 3 cut(s) 15, 1156, 1252
Kzo9I GATC 7 cut(s) 5, 15, 226, 331, 778, 1156, 1252
LguI GCTCTTC 1 cut(s) 271
LmnI GCTCC 1 cut(s) 403
Lsp1109I GCAGC 1 cut(s) 48
LweI GCATC 3 cut(s) 251, 385, 884
MaeI CTAG 3 cut(s) 450, 924, 1190
MaeII ACGT 4 cut(s) 12, 24, 177, 678
MaeIII GTNAC 4 cut(s) 122, 413, 452, 545
MalI GATC 7 cut(s) 7, 17, 228, 333, 780, 1158, 1254
MboI GATC 7 cut(s) 5, 15, 226, 331, 778, 1156, 1252
MboII GAAGA 5 cut(s) 185, 258, 598, 974, 1235
MfeI CAATTG 2 cut(s) 623, 1218
MflI RGATCY 1 cut(s) 331
MlyI GAGTC 5 cut(s) 212, 223, 499, 542, 929
MmeI TCCRAC 2 cut(s) 33, 96
MnlI CCTC 7 cut(s) 137, 240, 245, 267, 290, 1024, 1219
MseI TTAA 4 cut(s) 338, 443, 681, 882
MslI CAYNNNNRTG 1 cut(s) 1192
MspA1I CMGCKG 2 cut(s) 709, 1152
MspR9I CCNGG 1 cut(s) 119
MunI CAATTG 2 cut(s) 623, 1218
MvaI CCWGG 1 cut(s) 119
MwoI GCNNNNNNNGC 2 cut(s) 804, 1119
NcoI CCATGG 1 cut(s) 1238
NdeII GATC 7 cut(s) 5, 15, 226, 331, 778, 1156, 1252
NlaIII CATG 8 cut(s) 47, 366, 1163, 1168, 1197, 1210, 1242, 1329
NlaIV GGNNCC 1 cut(s) 861
NmuCI GTSAC 3 cut(s) 122, 452, 545
NspI RCATGY 1 cut(s) 47
NspV TTCGAA 1 cut(s) 1283
PaeI GCATGC 1 cut(s) 47
PagI TCATGA 1 cut(s) 1159
PciSI GCTCTTC 1 cut(s) 271
PfeI GAWTC 7 cut(s) 30, 167, 352, 612, 628, 970, 1202
PflMI CCANNNNNTGG 1 cut(s) 987
PkrI GCNGC 1 cut(s) 63
PleI GAGTC 5 cut(s) 211, 222, 499, 542, 928
PpsI GAGTC 5 cut(s) 211, 222, 499, 542, 928
PpuMI RGGWCCY 1 cut(s) 254
PsiI TTATAA 1 cut(s) 480
Psp5II RGGWCCY 1 cut(s) 254
Psp6I CCWGG 1 cut(s) 117
PspEI GGTNACC 1 cut(s) 122
PspFI CCCAGC 1 cut(s) 709
PspGI CCWGG 1 cut(s) 117
PspN4I GGNNCC 1 cut(s) 861
PspPI GGNCC 2 cut(s) 254, 296
PspPPI RGGWCCY 1 cut(s) 254
PsuI RGATCY 1 cut(s) 331
PvuII CAGCTG 1 cut(s) 709
RsaI GTAC 2 cut(s) 409, 1186
RsaNI GTAC 2 cut(s) 408, 1185
RseI CAYNNNNRTG 1 cut(s) 1192
SapI GCTCTTC 1 cut(s) 271
SaqAI TTAA 4 cut(s) 338, 443, 681, 882
SatI GCNGC 1 cut(s) 62
Sau3AI GATC 7 cut(s) 5, 15, 226, 331, 778, 1156, 1252
Sau96I GGNCC 2 cut(s) 254, 296
SchI GAGTC 5 cut(s) 212, 223, 499, 542, 929
ScrFI CCNGG 1 cut(s) 119
SfaNI GCATC 3 cut(s) 251, 385, 884
SfcI CTRYAG 2 cut(s) 327, 1147
SfuI TTCGAA 1 cut(s) 1283
SinI GGWCC 2 cut(s) 254, 296
SmiMI CAYNNNNRTG 1 cut(s) 1192
SphI GCATGC 1 cut(s) 47
SsiI CCGC 1 cut(s) 1152
SspI AATATT 1 cut(s) 1099
SspMI CTAG 3 cut(s) 450, 924, 1190
StyD4I CCNGG 1 cut(s) 117
StyI CCWWGG 1 cut(s) 1238
TaaI ACNGT 4 cut(s) 90, 186, 795, 935
TaiI ACGT 4 cut(s) 15, 27, 180, 681
TaqI TCGA 3 cut(s) 259, 819, 1283
TatI WGTACW 1 cut(s) 1184
TfiI GAWTC 7 cut(s) 30, 167, 352, 612, 628, 970, 1202
Tru1I TTAA 4 cut(s) 338, 443, 681, 882
Tru9I TTAA 4 cut(s) 338, 443, 681, 882
TseFI GTSAC 3 cut(s) 122, 452, 545
TseI GCWGC 1 cut(s) 61
Tsp45I GTSAC 3 cut(s) 122, 452, 545
TspDTI ATGAA 2 cut(s) 926, 1223
Van91I CCANNNNNTGG 1 cut(s) 987
VpaK11BI GGWCC 2 cut(s) 254, 296
XapI RAATTY 4 cut(s) 311, 319, 764, 895
XceI RCATGY 1 cut(s) 47
XspI CTAG 3 cut(s) 450, 924, 1190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.