FvH4_2g09700

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010976
Physical Location & Seq
Forward (+)
8581757 .. 8586389
4633 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g09700.t1

Sequence Viewer

Length: 2658 bp
ATGGAGATAGAAAAGACACCACCCAACAAGAAGTATCTTTTTCTATCCTCCTCGCCATCATCATCGTTAACAACCAGATACATGATGGCTATCCTCCTGGTCTTTGTATATGCACTCCTCTGCAACCCCACCATTGTCGTCTCCGAGCCTTCCTCCACCACCTTCACCCCGCAAGACAACATCCTCATTGCATGTGGTGCCAAAGATCCCCCCAAGCTACCCGATGGAAGGGTCTTCAAGACGGATGCACAAGCCAATCAATATTTGAAAGCAAATAATGATAACAAGGTGTCTGATGAAAAGGCAGATGTGCCTTCTCCTCTTTTCACCTCGGCTAGGATTTTCACAAGTGAAGCCACTTATACGTTCCACATGGGAAGGCCTGGGTGGCATTTTGTTCGCCTCTATTTCTTCCCCATTCAGAACTCTGTGTCTGATCTTCAGAAGGCAACCTTCAATGTCATGACAGATAAATACACTTTGCTCCATAGTTTCAATTTCAATGAGGCCGCTAAAAACAACACCCACAAAACTCTTTTCAATGAGTACCTTCTCAACGTGACCGAGCAACAATTCTCCATCAAGTTCTCTCCTGAGAAGAACTCCGCGGCATTCATCAATGCAATCGAGGTGGTTTCAGCTCCGGATTATCTCATCGGGGACACGGGTAACAACCTTCAACCATTGAACCAGTTCCAAGGATTGTCCAACTTTGGATATCAAACTATGTATAGGGTAAACATGGGAGGACCCTTGGTGACCTCGGTCAATGACACATTAGGACGAGAGTGGATACCTGACGAACAATTCATCAAGTCCAAGAACATGGCCAAAGTTGCCAAGGTTGAAAGTAAAGCTATCAAGTATCCTCAAGGGCTGACACCCCAAATAGCTCCACCATCAGTTTATTCAACCGCTGCGGAAATGGCAGATGAAGGTAATCAAAAGTTCAATGTAACATGGACCTTTCCGGCTGAAAATGCATATGGTTACTTGATCAGGTTGCATTTCTGTGACATTGTGAGCAAAACACTTGGTACCCTTTTCTTCAATGTCTACATTAATGGAAACGTGGCCATAAGTGAATTGGATTTGTCAGCAACCGTGAATGGTTTGGCAATTCCATTCTACAAGGACATTGTGGTCAACTCTTCATTGATAAACAACGATGGAGTCACTGTCCAAATCGGTCCGTCTAAATTGCAGAATGGTGTTCAAGATGCTATTCTAAATGGTGTAGAGGTCATGAGAATGAGCAATTCTGTTAATAGTTTGGATGGAGAGTTTGGAGTCGATGGAAAAGAGGCAAACCCAAGTAGCGGAGGTGGGGTTCACAAGGGAGCGGTGGCTGCAGTCGGATTTGGTTTGATGTTTGGAGCCTTCATAGGCCTTGGGGCAATGGTGTACAAATGGAAGAAGAGACCTCAAGATTGGCAGAAAAGGAACAGCTTCTCTTCTTGGTTACTGCCTGTACATGCTGGAGACACCAGCTTTATGTCAAGCAAGAACTCTGTGGGAAAGAACATGTACTCTTCCACTATGGGTCTTGGCCGATTCTTCTCTTTCGCAGAGTTACAGGAAGCTACCAACAATTTTGACTCGAGCAAAATCATTGGTGTTGGTGGATTTGGCAATGTGTATATTGGTGTGATTGATGATGGGACTAGTGTTGCGGTCAAGAGAGGAAACCCTCAATCTGAACAGGGGATTACGGAGTTCCAAACAGAAATCCAAATGTTGTCGAAGCTCAGGCACAGACATCTGGTCTCTTTAATTGGCTATTGTGATGAAAACGAAGAGATGATCTTGGTTTATGAGTTCATGTCAAATGGACCATTCAGAGATCATCTGTATGGTAAGGACTTGCCACCATTGTCATGGAAACAAAGATTGGAGATATGCATCGGCGCTGCTCGTGGGCTTCACTACCTTCATACGGGAACAGCACAAGGCATCATCCACCGCGATGTTAAGACAACAAACATCCTTCTCGATGATCAGTTCACCGCTAAGGTTGCTGATTTCGGGCTATCTAAAGATGCGCCAATGGGACAGAACCATGTGAGTACCGCTGTGAAGGGAAGCTTTGGGTACTTAGATCCTGAGTACTTTAGGAGACAGCAATTGACAGACAAATCCGATGTCTACTCATTCGGAGTTGTCATGCTCGAGGCATTGTGCGCTAGGCCTGCTATCAATCCAGCACTTCCAAGAGAGCAAGTCAACTTGGCCGATTGGGGAATGCAGTGGAAGAGGAAGGGCTTGTTGGAAAAGATAGTGGACCCTCTACTCATCGGAACCATCAACCCTGAATCCATGAAGAAGTTTGGTGAGGCTGCCGAGAAGTGCTTACTAGAGCATGGTGTGGATAGGCCTACAATGGGAGATGTGCTGTGGAACTTGGAGTATGCCCTGCAACTTCAAGAGGCGTTCACAGAAGCAAAGGGTGAAGATGATAGCGTTACCGTCAACGCTCCTGCTGATGCTGCAGCTGCCTCTCTAGCTGATGCTGATGCTGCTGCTTCTACTGCCGCAGCCTCGGTACCTCACCCAGCTTCTATCTTAGAGGAGGAAGAAGCTCCCCCTGCAGAACAGACAGTAAATGACCAATCTGGAACAGCTATGTTTGCTCAATTTTCTAACCTCAATGGTAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Malectin_like Pkinase_Tyr

Protein Analysis

886

Amino Acids

97.2

Weight (kDa)

5.47

Isoelectric Point (pI)

36.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin PF11721 62 - 207 6.1e-07 Malectin domain
Malectin_like PF12819 63 - 417 7.8e-26 Malectin-like domain
Malectin PF11721 243 - 383 1.2e-09 Malectin domain
Pkinase PF00069 534 - 733 3.3e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 535 - 727 3.8e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010976)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21480 AT4G39110
fragaria_vesca FvH4_2g09700
malus_domestica MD05G1060800.v1.1 MD10G1068600.v1.1
prunus_persica Prupe.8G090600_v2.0.a1
pyrus_communis pycom10g05470
rosa_chinensis RchiOBHm_Chr6g0266011
rosa_laevigata RLG00000014135 RLG00000014136 RLG00000014138
rosa_multiflora Rmu_sc0000776.1_g000006
rosa_roxburghii Rroxscaffold_174G00435080 Rroxscaffold_7G00202100
rosa_rugosa Rorug06G0022500
rosa_samantha Rh6AG143700 Rh6BG144000 Rh6CG140700 Rh6DG128300
rosa_wichuraiana Rw6G012420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1142
Acc65I GGTACC 2 cut(s) 1037, 2542
AccB1I GGYRCC 3 cut(s) 197, 1037, 2542
AccBSI CCGCTC 1 cut(s) 1343
AccI GTMKAC 2 cut(s) 1056, 2145
AccII CGCG 2 cut(s) 608, 1965
AccIII TCCGGA 1 cut(s) 643
AclWI GGATC 2 cut(s) 200, 2093
AcoI YGGCCR 4 cut(s) 828, 1074, 1549, 2229
AcuI CTGAAG 1 cut(s) 425
AfaI GTAC 9 cut(s) 548, 1039, 1406, 1473, 1529, 2068, 2093, 2108, 2544
AfiI CCNNNNNNNGG 5 cut(s) 228, 336, 1319, 1936, 2381
AflIII ACRYGT 1 cut(s) 1524
AhdI GACNNNNNGTC 1 cut(s) 1763
AhlI ACTAGT 1 cut(s) 1664
AjnI CCWGG 2 cut(s) 96, 382
AjuI GAANNNNNNNTTGG 4 cut(s) 1874, 1906, 2249, 2281
AloI GAACNNNNNNTCC 2 cut(s) 1314, 1346
Alw26I GTCTC 5 cut(s) 145, 1414, 1476, 1771, 2110
AlwI GGATC 2 cut(s) 200, 2093
Ama87I CYCGRG 2 cut(s) 1600, 2168
Aor13HI TCCGGA 1 cut(s) 643
AoxI GGCC 9 cut(s) 380, 507, 828, 1074, 1387, 1549, 2186, 2229, 2372
ArsI GACNNNNNNTTYG 2 cut(s) 2127, 2159
AseI ATTAAT 1 cut(s) 1062
Asp700I GAANNNNTTC 2 cut(s) 692, 1448
Asp718I GGTACC 2 cut(s) 1037, 2542
AspLEI GCGC 3 cut(s) 1910, 2044, 2183
AspS9I GGNCC 5 cut(s) 749, 963, 1190, 1832, 2281
AsuHPI GGTGA 7 cut(s) 157, 319, 769, 1996, 2342, 2459, 2540
AvaI CYCGRG 2 cut(s) 1600, 2168
AvaII GGWCC 5 cut(s) 749, 963, 1190, 1832, 2281
BalI TGGCCA 2 cut(s) 830, 1076
BanI GGYRCC 3 cut(s) 197, 1037, 2542
BauI CACGAG 1 cut(s) 1914
BbsI GAAGAC 1 cut(s) 226
BcgI CGANNNNNNTGC 2 cut(s) 380, 414
BciT130I CCWGG 2 cut(s) 98, 384
BciVI GTATCC 2 cut(s) 786, 876
BclI TGATCA 2 cut(s) 996, 1996
BcoDI GTCTC 5 cut(s) 145, 1414, 1476, 1771, 2110
BcuI ACTAGT 1 cut(s) 1664
BfaI CTAG 5 cut(s) 336, 1665, 2184, 2354, 2501
BfmI CTRYAG 3 cut(s) 1350, 2487, 2586
BfoI RGCGCY 1 cut(s) 1911
BfuI GTATCC 2 cut(s) 786, 876
BglI GCCNNNNNGGC 1 cut(s) 388
BmcAI AGTACT 1 cut(s) 2108
Bme1390I CCNGG 2 cut(s) 98, 384
Bme18I GGWCC 5 cut(s) 749, 963, 1190, 1832, 2281
BmeRI GACNNNNNGTC 1 cut(s) 1763
BmeT110I CYCGRG 2 cut(s) 1600, 2168
BmgT120I GGNCC 5 cut(s) 749, 963, 1190, 1832, 2281
BmiI GGNNCC 7 cut(s) 199, 751, 1039, 1378, 2283, 2299, 2544
BmrFI CCNGG 2 cut(s) 98, 384
BmsI GCATC 8 cut(s) 235, 1210, 1911, 1962, 2029, 2473, 2497, 2503
BoxI GACNNNNGTC 1 cut(s) 764
BpiI GAAGAC 1 cut(s) 226
BplI GAGNNNNNCTC 4 cut(s) 137, 169, 587, 619
BpmI CTGGAG 1 cut(s) 1500
Bpu10I CCTNAGC 2 cut(s) 1748, 2010
BpuEI CTTGAG 2 cut(s) 855, 1410
BsaBI GATNNNNATC 2 cut(s) 89, 1901
BsaI GGTCTC 2 cut(s) 1414, 1771
BsaJI CCNNGG 9 cut(s) 330, 383, 606, 697, 753, 762, 840, 1390, 2538
BsaWI WCCGGW 1 cut(s) 643
BsaXI ACNNNNNCTCC 4 cut(s) 1164, 1194, 1314, 1344
Bsc4I CCNNNNNNNGG 5 cut(s) 228, 336, 1319, 1936, 2381
Bse1I ACTGG 1 cut(s) 691
Bse3DI GCAATG 3 cut(s) 186, 1404, 1639
Bse8I GATNNNNATC 2 cut(s) 89, 1901
BseAI TCCGGA 1 cut(s) 643
BseBI CCWGG 2 cut(s) 98, 384
BseDI CCNNGG 9 cut(s) 330, 383, 606, 697, 753, 762, 840, 1390, 2538
BseGI GGATG 5 cut(s) 180, 250, 1282, 1956, 1983
BseJI GATNNNNATC 2 cut(s) 89, 1901
BseLI CCNNNNNNNGG 5 cut(s) 228, 336, 1319, 1936, 2381
BseMI GCAATG 3 cut(s) 186, 1404, 1639
BseMII CTCAG 3 cut(s) 585, 1762, 2094
BseNI ACTGG 1 cut(s) 691
BseRI GAGGAG 4 cut(s) 40, 107, 309, 2582
BseYI CCCAGC 1 cut(s) 2551
Bsh1236I CGCG 2 cut(s) 608, 1965
BshFI GGCC 9 cut(s) 382, 509, 830, 1076, 1389, 1551, 2188, 2231, 2374
BshNI GGYRCC 3 cut(s) 197, 1037, 2542
BsiHKCI CYCGRG 2 cut(s) 1600, 2168
BsiSI CCGG 2 cut(s) 644, 971
BslFI GGGAC 3 cut(s) 674, 1675, 2064
BslI CCNNNNNNNGG 5 cut(s) 228, 336, 1319, 1936, 2381
BsmAI GTCTC 5 cut(s) 145, 1414, 1476, 1771, 2110
BsmBI CGTCTC 1 cut(s) 145
BsmFI GGGAC 3 cut(s) 674, 1675, 2064
BsmI GAATGC 2 cut(s) 611, 2247
BsnI GGCC 9 cut(s) 382, 509, 830, 1076, 1389, 1551, 2188, 2231, 2374
Bso31I GGTCTC 2 cut(s) 1414, 1771
BsoBI CYCGRG 2 cut(s) 1600, 2168
Bsp13I TCCGGA 1 cut(s) 643
Bsp1407I TGTACA 2 cut(s) 1404, 1471
Bsp143I GATC 7 cut(s) 205, 436, 996, 1803, 1843, 1996, 2098
BspANI GGCC 9 cut(s) 382, 509, 830, 1076, 1389, 1551, 2188, 2231, 2374
BspCNI CTCAG 3 cut(s) 586, 1761, 2095
BspEI TCCGGA 1 cut(s) 643
BspFNI CGCG 2 cut(s) 608, 1965
BspHI TCATGA 2 cut(s) 462, 1245
BspLI GGNNCC 7 cut(s) 199, 751, 1039, 1378, 2283, 2299, 2544
BspMAI CTGCAG 3 cut(s) 1354, 2491, 2590
BspPI GGATC 2 cut(s) 200, 2093
BspT107I GGYRCC 3 cut(s) 197, 1037, 2542
BspTNI GGTCTC 2 cut(s) 1414, 1771
BsrBI CCGCTC 1 cut(s) 1343
BsrDI GCAATG 3 cut(s) 186, 1404, 1639
BsrGI TGTACA 2 cut(s) 1404, 1471
BsrI ACTGG 1 cut(s) 691
BssECI CCNNGG 9 cut(s) 330, 383, 606, 697, 753, 762, 840, 1390, 2538
BssMI GATC 7 cut(s) 205, 436, 996, 1803, 1843, 1996, 2098
BssSI CACGAG 1 cut(s) 1914
BssT1I CCWWGG 4 cut(s) 697, 753, 840, 1390
Bst2BI CACGAG 1 cut(s) 1914
Bst2UI CCWGG 2 cut(s) 98, 384
Bst4CI ACNGT 4 cut(s) 1105, 1180, 2467, 2599
Bst6I CTCTTC 6 cut(s) 1156, 1412, 1459, 1537, 1791, 2246
BstAPI GCANNNNNTGC 1 cut(s) 197
BstAUI TGTACA 2 cut(s) 1404, 1471
BstC8I GCNNGC 1 cut(s) 2190
BstDEI CTNAG 6 cut(s) 594, 1748, 2010, 2095, 2103, 2563
BstDSI CCRYGG 1 cut(s) 606
BstEII GGTNACC 1 cut(s) 757
BstENI CCTNNNNNAGG 1 cut(s) 334
BstF5I GGATG 5 cut(s) 180, 250, 1282, 1956, 1983
BstFNI CGCG 2 cut(s) 608, 1965
BstH2I RGCGCY 1 cut(s) 1911
BstHHI GCGC 3 cut(s) 1910, 2044, 2183
BstKTI GATC 7 cut(s) 208, 439, 999, 1806, 1846, 1999, 2101
BstMAI GTCTC 5 cut(s) 145, 1414, 1476, 1771, 2110
BstMBI GATC 7 cut(s) 205, 436, 996, 1803, 1843, 1996, 2098
BstNI CCWGG 2 cut(s) 98, 384
BstNSI RCATGY 3 cut(s) 195, 1478, 1528
BstPAI GACNNNNGTC 1 cut(s) 764
BstPI GGTNACC 1 cut(s) 757
BstSCI CCNGG 2 cut(s) 96, 382
BstSFI CTRYAG 3 cut(s) 1350, 2487, 2586
BstUI CGCG 2 cut(s) 608, 1965
BstV2I GAAGAC 1 cut(s) 226
BstX2I RGATCY 2 cut(s) 205, 2098
BstXI CCANNNNNNTGG 2 cut(s) 826, 1878
BstYI RGATCY 2 cut(s) 205, 2098
BsuI GTATCC 2 cut(s) 786, 876
BsuRI GGCC 9 cut(s) 382, 509, 830, 1076, 1389, 1551, 2188, 2231, 2374
BtgI CCRYGG 1 cut(s) 606
BtgZI GCGATG 1 cut(s) 1980
BtsCI GGATG 5 cut(s) 180, 250, 1282, 1956, 1983
BtsI GCAGTG 1 cut(s) 2252
BtsIMutI CAGTG 2 cut(s) 1176, 2252
Cac8I GCNNGC 1 cut(s) 2190
CciI TCATGA 2 cut(s) 462, 1245
CfoI GCGC 3 cut(s) 1910, 2044, 2183
Cfr13I GGNCC 5 cut(s) 749, 963, 1190, 1832, 2281
Cfr42I CCGCGG 1 cut(s) 609
CpoI CGGWCCG 1 cut(s) 1190
Csp6I GTAC 9 cut(s) 547, 1038, 1405, 1472, 1528, 2067, 2092, 2107, 2543
CspCI CAANNNNNGTGG 2 cut(s) 612, 647
CspI CGGWCCG 1 cut(s) 1190
CviQI GTAC 9 cut(s) 547, 1038, 1405, 1472, 1528, 2067, 2092, 2107, 2543
DdeI CTNAG 6 cut(s) 594, 1748, 2010, 2095, 2103, 2563
DpnI GATC 7 cut(s) 207, 438, 998, 1805, 1845, 1998, 2100
DpnII GATC 7 cut(s) 205, 436, 996, 1803, 1843, 1996, 2098
DrdI GACNNNNNNGTC 1 cut(s) 1142
DriI GACNNNNNGTC 1 cut(s) 1763
DseDI GACNNNNNNGTC 1 cut(s) 1142
EaeI YGGCCR 4 cut(s) 828, 1074, 1549, 2229
Eam1104I CTCTTC 6 cut(s) 1156, 1412, 1459, 1537, 1791, 2246
Eam1105I GACNNNNNGTC 1 cut(s) 1763
EarI CTCTTC 6 cut(s) 1156, 1412, 1459, 1537, 1791, 2246
Eco130I CCWWGG 4 cut(s) 697, 753, 840, 1390
Eco147I AGGCCT 4 cut(s) 382, 1389, 2188, 2374
Eco31I GGTCTC 2 cut(s) 1414, 1771
Eco32I GATATC 1 cut(s) 719
Eco47I GGWCC 5 cut(s) 749, 963, 1190, 1832, 2281
Eco57I CTGAAG 1 cut(s) 425
Eco88I CYCGRG 2 cut(s) 1600, 2168
Eco91I GGTNACC 1 cut(s) 757
EcoNI CCTNNNNNAGG 1 cut(s) 334
EcoO109I RGGNCCY 1 cut(s) 749
EcoO65I GGTNACC 1 cut(s) 757
EcoRII CCWGG 2 cut(s) 96, 382
EcoRV GATATC 1 cut(s) 719
EcoT14I CCWWGG 4 cut(s) 697, 753, 840, 1390
EcoT22I ATGCAT 2 cut(s) 985, 1904
ErhI CCWWGG 4 cut(s) 697, 753, 840, 1390
Esp3I CGTCTC 1 cut(s) 145
FalI AAGNNNNNCTT 4 cut(s) 437, 469, 2069, 2101
FaqI GGGAC 3 cut(s) 674, 1675, 2064
FauI CCCGC 1 cut(s) 177
FauNDI CATATG 1 cut(s) 985
FbaI TGATCA 2 cut(s) 996, 1996
FblI GTMKAC 2 cut(s) 1056, 2145
FokI GGATG 5 cut(s) 167, 257, 1289, 1943, 1970
FspBI CTAG 5 cut(s) 336, 1665, 2184, 2354, 2501
GlaI GCGC 3 cut(s) 1909, 2043, 2182
GsaI CCCAGC 1 cut(s) 2555
GsuI CTGGAG 1 cut(s) 1500
HaeII RGCGCY 1 cut(s) 1911
HaeIII GGCC 9 cut(s) 382, 509, 830, 1076, 1389, 1551, 2188, 2231, 2374
HapII CCGG 2 cut(s) 644, 971
HhaI GCGC 3 cut(s) 1910, 2044, 2183
Hin6I GCGC 3 cut(s) 1908, 2042, 2181
HinP1I GCGC 3 cut(s) 1908, 2042, 2181
HincII GTYRAC 4 cut(s) 69, 1147, 2224, 2470
HindII GTYRAC 4 cut(s) 69, 1147, 2224, 2470
HindIII AAGCTT 1 cut(s) 2083
HinfI GANTC 5 cut(s) 1173, 1290, 1554, 1598, 2312
HpaI GTTAAC 1 cut(s) 69
HpaII CCGG 2 cut(s) 644, 971
HphI GGTGA 7 cut(s) 157, 319, 769, 1996, 2342, 2459, 2540
HpyCH4III ACNGT 4 cut(s) 1105, 1180, 2467, 2599
HpyCH4IV ACGT 3 cut(s) 365, 558, 1071
HpyF3I CTNAG 6 cut(s) 594, 1748, 2010, 2095, 2103, 2563
HpySE526I ACGT 3 cut(s) 365, 558, 1071
HspAI GCGC 3 cut(s) 1908, 2042, 2181
Kpn2I TCCGGA 1 cut(s) 643
KpnI GGTACC 2 cut(s) 1041, 2546
Ksp22I TGATCA 2 cut(s) 996, 1996
KspAI GTTAAC 1 cut(s) 69
KspI CCGCGG 1 cut(s) 609
Kzo9I GATC 7 cut(s) 205, 436, 996, 1803, 1843, 1996, 2098
LmnI GCTCC 7 cut(s) 489, 646, 898, 1340, 1376, 2479, 2584
LweI GCATC 8 cut(s) 235, 1210, 1911, 1962, 2029, 2473, 2497, 2503
MaeI CTAG 5 cut(s) 336, 1665, 2184, 2354, 2501
MaeII ACGT 3 cut(s) 365, 558, 1071
MalI GATC 7 cut(s) 207, 438, 998, 1805, 1845, 1998, 2100
MbiI CCGCTC 1 cut(s) 1343
MboI GATC 7 cut(s) 205, 436, 996, 1803, 1843, 1996, 2098
MfeI CAATTG 1 cut(s) 2123
MflI RGATCY 2 cut(s) 205, 2098
MlsI TGGCCA 2 cut(s) 830, 1076
MluNI TGGCCA 2 cut(s) 830, 1076
MlyI GAGTC 3 cut(s) 1182, 1299, 1592
MmeI TCCRAC 3 cut(s) 732, 1336, 2247
Mox20I TGGCCA 2 cut(s) 830, 1076
Mph1103I ATGCAT 2 cut(s) 985, 1904
MroI TCCGGA 1 cut(s) 643
MroXI GAANNNNTTC 2 cut(s) 692, 1448
MscI TGGCCA 2 cut(s) 830, 1076
MseI TTAA 5 cut(s) 68, 1062, 1266, 1772, 1971
MslI CAYNNNNRTG 5 cut(s) 1011, 1250, 1851, 1876, 1965
Msp20I TGGCCA 2 cut(s) 830, 1076
MspA1I CMGCKG 4 cut(s) 608, 917, 2072, 2492
MspI CCGG 2 cut(s) 644, 971
MspR9I CCNGG 2 cut(s) 98, 384
MunI CAATTG 1 cut(s) 2123
Mva1269I GAATGC 2 cut(s) 611, 2247
MvaI CCWGG 2 cut(s) 98, 384
MvnI CGCG 2 cut(s) 608, 1965
NdeI CATATG 1 cut(s) 985
NdeII GATC 7 cut(s) 205, 436, 996, 1803, 1843, 1996, 2098
NlaIV GGNNCC 7 cut(s) 199, 751, 1039, 1378, 2283, 2299, 2544
NmeAIII GCCGAG 2 cut(s) 311, 2365
NmuCI GTSAC 4 cut(s) 559, 757, 1013, 1174
NsiI ATGCAT 2 cut(s) 985, 1904
NspI RCATGY 3 cut(s) 195, 1478, 1528
PaeR7I CTCGAG 2 cut(s) 1600, 2168
PagI TCATGA 2 cut(s) 462, 1245
PceI AGGCCT 4 cut(s) 382, 1389, 2188, 2374
PciI ACATGT 1 cut(s) 1524
PctI GAATGC 2 cut(s) 611, 2247
PdmI GAANNNNTTC 2 cut(s) 692, 1448
PfeI GAWTC 2 cut(s) 1554, 2312
PleI GAGTC 3 cut(s) 1181, 1298, 1592
PpsI GAGTC 3 cut(s) 1181, 1298, 1592
PpuMI RGGWCCY 1 cut(s) 749
PscI ACATGT 1 cut(s) 1524
PshAI GACNNNNGTC 1 cut(s) 764
PshBI ATTAAT 1 cut(s) 1062
Psp5II RGGWCCY 1 cut(s) 749
Psp6I CCWGG 2 cut(s) 96, 382
PspEI GGTNACC 1 cut(s) 757
PspFI CCCAGC 1 cut(s) 2551
PspGI CCWGG 2 cut(s) 96, 382
PspN4I GGNNCC 7 cut(s) 199, 751, 1039, 1378, 2283, 2299, 2544
PspPI GGNCC 5 cut(s) 749, 963, 1190, 1832, 2281
PspPPI RGGWCCY 1 cut(s) 749
PspXI VCTCGAGB 2 cut(s) 1600, 2168
PstI CTGCAG 3 cut(s) 1354, 2491, 2590
PsuI RGATCY 2 cut(s) 205, 2098
PvuII CAGCTG 1 cut(s) 2492
RsaI GTAC 9 cut(s) 548, 1039, 1406, 1473, 1529, 2068, 2093, 2108, 2544
RsaNI GTAC 9 cut(s) 547, 1038, 1405, 1472, 1528, 2067, 2092, 2107, 2543
RseI CAYNNNNRTG 5 cut(s) 1011, 1250, 1851, 1876, 1965
Rsr2I CGGWCCG 1 cut(s) 1190
RsrII CGGWCCG 1 cut(s) 1190
SacII CCGCGG 1 cut(s) 609
SaqAI TTAA 5 cut(s) 68, 1062, 1266, 1772, 1971
Sau3AI GATC 7 cut(s) 205, 436, 996, 1803, 1843, 1996, 2098
Sau96I GGNCC 5 cut(s) 749, 963, 1190, 1832, 2281
ScaI AGTACT 1 cut(s) 2108
SchI GAGTC 3 cut(s) 1182, 1299, 1592
ScrFI CCNGG 2 cut(s) 98, 384
SfaNI GCATC 8 cut(s) 235, 1210, 1911, 1962, 2029, 2473, 2497, 2503
SfcI CTRYAG 3 cut(s) 1350, 2487, 2586
Sfr274I CTCGAG 2 cut(s) 1600, 2168
Sfr303I CCGCGG 1 cut(s) 609
SgrBI CCGCGG 1 cut(s) 609
SinI GGWCC 5 cut(s) 749, 963, 1190, 1832, 2281
SlaI CTCGAG 2 cut(s) 1600, 2168
SmiMI CAYNNNNRTG 5 cut(s) 1011, 1250, 1851, 1876, 1965
SmlI CTYRAG 4 cut(s) 870, 1425, 1600, 2168
SmoI CTYRAG 4 cut(s) 870, 1425, 1600, 2168
SpeI ACTAGT 1 cut(s) 1664
SseBI AGGCCT 4 cut(s) 382, 1389, 2188, 2374
SspI AATATT 1 cut(s) 263
SspMI CTAG 5 cut(s) 336, 1665, 2184, 2354, 2501
StuI AGGCCT 4 cut(s) 382, 1389, 2188, 2374
StyD4I CCNGG 2 cut(s) 96, 382
StyI CCWWGG 4 cut(s) 697, 753, 840, 1390
TaaI ACNGT 4 cut(s) 1105, 1180, 2467, 2599
TaiI ACGT 3 cut(s) 368, 561, 1074
TaqI TCGA 6 cut(s) 627, 1293, 1601, 1742, 1992, 2169
TaqII GACCGA 3 cut(s) 578, 754, 1178
TatI WGTACW 4 cut(s) 1404, 1471, 1527, 2106
TauI GCSGC 3 cut(s) 512, 611, 2534
TfiI GAWTC 2 cut(s) 1554, 2312
Tru1I TTAA 5 cut(s) 68, 1062, 1266, 1772, 1971
Tru9I TTAA 5 cut(s) 68, 1062, 1266, 1772, 1971
TscAI CASTG 2 cut(s) 1183, 2252
TseFI GTSAC 4 cut(s) 559, 757, 1013, 1174
Tsp45I GTSAC 4 cut(s) 559, 757, 1013, 1174
TspGWI ACGGA 3 cut(s) 257, 1182, 1727
TspRI CASTG 2 cut(s) 1183, 2252
VpaK11BI GGWCC 5 cut(s) 749, 963, 1190, 1832, 2281
VspI ATTAAT 1 cut(s) 1062
XagI CCTNNNNNAGG 1 cut(s) 334
XceI RCATGY 3 cut(s) 195, 1478, 1528
XcmI CCANNNNNNNNNTGG 3 cut(s) 82, 1084, 1875
XhoI CTCGAG 2 cut(s) 1600, 2168
XmiI GTMKAC 2 cut(s) 1056, 2145
XmnI GAANNNNTTC 2 cut(s) 692, 1448
XspI CTAG 5 cut(s) 336, 1665, 2184, 2354, 2501
ZrmI AGTACT 1 cut(s) 2108
Zsp2I ATGCAT 2 cut(s) 985, 1904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.