RLG00000014138

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0010976
Physical Location & Seq
Reverse (-)
47936413 .. 47939082
2670 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014138

Sequence Viewer

Length: 2670 bp
ATGGAGATAGAAAAGACACCAAACGAACCCAAAAAGAAGTATCTTTTTCTATCCTCCTTGCCATCATTATCATCATCATTAACGTCGAGATACATGATGGCTATCCTCCTGGTCTTTGTTTATGCACTTCTCTACAACCCCACCATTGTCCTCTCTGACCCTGCTTCCCCCACCACCTTCACCCCCCAAGACAACTTCCTCATCGACTGCGGTGCTGCAAACGCTCCCACACTACCCGATGGAAGGACCTTCAAGACTGATTCACAATCATCGCAACATTTGAAAGCAAATGATGATAACAAGGTGTCTGATGACAAGGCTGATGTGCCTTCTCCTCTTTACAAAACCGCAAGGATTTTCACAAGTGAAGCCACTTATACGTTCCACATGGGAAGTCCTGGGTGGCATTGGGTTCGCCTCTATTTCTATCCCATTCAAAACTCTGTGTCTGATCTTCAGAAGGCAACTTTCAATGTCATGACAGATAAGTACACTTTGCTCCATAGCTTCAATTTCAATGAGGCCGCTAACAACAACACCATCAAAGTTGTTTACAAGGAGTACCTTCTCAACGTGACCGAGCAGCAATTCTCGATCAAGTTCTCTCCCCAGAAGAACTCCGCGGCGTTCATCAATGCAATTGAGGTGGTTTCAGCTCCAGATTACCTCATCTCGGACACGGCTAACAACCTTCAGCCAGTGAGCCAGTTCCAGGGATTGTCCAAATTTGGATACGAATATATGTACAGGATTAACGTGGGAGGACCTCTAGTGACCCCGGTCAATGACACATTAGGACGGTTCTGGTTGCCGGACGAGCCATACATTAAGTCCAAGAACATGGCCCAAAGTGCCAAGGTTGAAACCAAAACTATCAAGTATCCTCAAGGGCTTACACCCCAAATAGCTCCACCAATGGTTTATGCAACTGCTGCGCATATGGAGGATGCAGGTGTCAGCAATCAAAAGTTCAATGTGACATGGGACTTTCAGGCTGAAAATGCATATGGTTACTTGATCCGTTTGCATTTCTGCGACATTGTGAGCAAAACACTTGGTAATCTCTACTTCAATGTCTACATCAATGGGCAGATGGCAATAAGTGATTTGGATTTGTCACAAACCGTGAACGGTTTGGCAATTCCATTCTACAAGGACATTGTGGTCAACTCTTCATTGATAAAAGACGCGCTTTCAATCCAGATTGGTCCGTCTAAATTGGGGAGTGGTGCTCAGGATGCCATTCTAAATGGTTTGGAGGTTATGAAAATAAGCAATTCTGTTAATAGTTTGGATGGAGAGTTCGGAGTCGATGGAAAAGAATCAAAAGATAGCAGCGGAGGTGTTCACAAGGGAGCGGTGGCTGCAGTCGGATTTGGTTTGATGTTTGGAGCCTTTATTGGACTTGGGGCAATGGTGTACAAATGGAAGAAGAGACCTCAAGATTGGCAGAAAAGGAACAGCTTCTCTTCTTGGTTACTTCCTGTACATGCTGGAGACAGCAGTTTTATGTCAAGCAAGAACTCCGTGGGAAAAAGCATGTACTCTTCCTCTATGGGCCTTGGCCGATTCTTCTCTCTCGCGGAGTTACAGGAAGCTACCAACAATTTCGACTCGAACAAAATCATTGGTGTTGGTGGATTTGGCAATGTGTATCTTGGCGTGATTGATGATGGGATTAGTGTTGCTGTCAAGAGAGGAAATCCACAATCTGAACAGGGGATTACAGAGTTCCAAACAGAGATTCAAATGTTGTCAAAGCTGAGGCACAGGCATTTGGTCTCTCTAATTGGCTATTGTGATGAAAACCAAGAGATGATCTTGGTTTATGAGTTCATGTCAAATGGACCATTCAGAGATCATCTATATGGTAAGAACTTGCCACCACTGTCATGGAAACAAAGACTAGAGATCTGCATTGGCGCTGCTCGCGGGCTTCACTACCTTCATACTGGCACAGCACAAGGCATCATTCACCGCGATGTGAAGACAACAAACATCCTTCTCGATGACCAGTTCACTGCCAAGGTTGCTGATTTCGGGTTGTCTAAAGATGCGCCAATGGGACAGAACCATGTGAGTACCGCCGTGAAGGGAAGCTTTGGGTACTTGGACCCTGAGTACTTTAGGAGGCAGCAACTGACAGACAAATCCGATGTCTACTCATTCGGAGTTGTCATGCTCGAGGCATTGTGCGCTAGGCCTGCGATAAATCCAGCACTTCCAAGAGAGCAAGTCAACTTGGCCGACTGGGGAATGCAGTGGAAGAGGAAAGGCTTGCTGGAAAAGATTGTCGACCCTCTCCTCATTGGAACCATCAACCCTGAATCCATGAAGAAGTTTGGTGAGGCTGCCGAGAAGTGCTTGTTAGAGCACGGTGTGGATAGGCCTACGATGGGAGATGTGCTGTGGAACTTGGAGTATGCTCTGCAGCTTCAAGAGGCATTCACCGAAGGAAAGGGCGAAGATGACAGCATTAATGCTACCCCCTCCGCTGATGCTGCATCTGCCTCTCCTACTGATGCCGCTGCTGCTGCTCCTACTGCCGCTGCCTCCGTTCCTCTCCCGGCTGCTATCCTGGAGGAAGAAGCCCCCCCTACAGAACAGACTATTAATGATCAATCTGGAACTGCTATATTGTCTCAATTTTCTAACCTCAATGGTAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Malectin_like Pkinase_Tyr

Protein Analysis

890

Amino Acids

97.79

Weight (kDa)

5.62

Isoelectric Point (pI)

39.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin PF11721 67 - 212 9.7e-10 Malectin domain
Malectin_like PF12819 68 - 424 1.3e-29 Malectin-like domain
Malectin PF11721 248 - 402 2.1e-09 Malectin domain
Pkinase PF00069 538 - 738 1.3e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 540 - 732 1.2e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010976)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21480 AT4G39110
fragaria_vesca FvH4_2g09700
malus_domestica MD05G1060800.v1.1 MD10G1068600.v1.1
prunus_persica Prupe.8G090600_v2.0.a1
pyrus_communis pycom10g05470
rosa_chinensis RchiOBHm_Chr6g0266011
rosa_laevigata RLG00000014135 RLG00000014136 RLG00000014138
rosa_multiflora Rmu_sc0000776.1_g000006
rosa_roxburghii Rroxscaffold_174G00435080 Rroxscaffold_7G00202100
rosa_rugosa Rorug06G0022500
rosa_samantha Rh6AG143700 Rh6BG144000 Rh6CG140700 Rh6DG128300
rosa_wichuraiana Rw6G012420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 941
AasI GACNNNNNNGTC 1 cut(s) 1163
Acc16I TGCGCA 1 cut(s) 936
Acc36I ACCTGC 1 cut(s) 941
AccBSI CCGCTC 1 cut(s) 1358
AccI GTMKAC 3 cut(s) 1077, 2160, 2295
AccII CGCG 5 cut(s) 623, 1190, 1583, 1932, 1980
AclWI GGATC 1 cut(s) 1012
AcoI YGGCCR 2 cut(s) 1564, 2244
AcsI RAATTY 1 cut(s) 725
AcuI CTGAAG 2 cut(s) 440, 677
AdeI CACNNNGTG 1 cut(s) 2380
AfaI GTAC 9 cut(s) 491, 563, 746, 1421, 1488, 1544, 2083, 2108, 2123
AfiI CCNNNNNNNGG 4 cut(s) 243, 673, 712, 2396
AjnI CCWGG 4 cut(s) 108, 397, 711, 2577
AjuI GAANNNNNNNTTGG 2 cut(s) 1728, 1760
AloI GAACNNNNNNTCC 2 cut(s) 1286, 1318
AluBI AGCT 8 cut(s) 507, 656, 908, 1464, 1598, 1762, 2100, 2434
AluI AGCT 8 cut(s) 507, 656, 908, 1464, 1598, 1762, 2100, 2434
Alw21I GWGCWC 2 cut(s) 1234, 2376
Alw26I GTCTC 4 cut(s) 1429, 1491, 1786, 2646
AlwI GGATC 1 cut(s) 1012
AlwNI CAGNNNCTG 1 cut(s) 2140
Ama87I CYCGRG 1 cut(s) 2183
AoxI GGCC 7 cut(s) 522, 843, 1558, 1564, 2201, 2244, 2387
ApoI RAATTY 1 cut(s) 725
ArsI GACNNNNNNTTYG 2 cut(s) 2142, 2174
AseI ATTAAT 2 cut(s) 2478, 2613
Asp700I GAANNNNTTC 1 cut(s) 1463
AspLEI GCGC 5 cut(s) 937, 1192, 1925, 2059, 2198
AspS9I GGNCC 7 cut(s) 246, 764, 844, 1208, 1558, 1847, 2113
AsuC2I CCSGG 2 cut(s) 779, 2567
AsuHPI GGTGA 4 cut(s) 172, 1967, 2357, 2440
AvaI CYCGRG 1 cut(s) 2183
AvaII GGWCC 5 cut(s) 246, 764, 1208, 1847, 2113
BbsI GAAGAC 1 cut(s) 1994
Bbv12I GWGCWC 2 cut(s) 1234, 2376
BbvCI CCTCAGC 1 cut(s) 1763
BceAI ACGGC 2 cut(s) 696, 2072
BcgI CGANNNNNNTGC 4 cut(s) 194, 228, 395, 429
BciT130I CCWGG 4 cut(s) 110, 399, 713, 2579
BciVI GTATCC 2 cut(s) 725, 891
BclI TGATCA 1 cut(s) 2617
BcnI CCSGG 2 cut(s) 779, 2567
BcoDI GTCTC 4 cut(s) 1429, 1491, 1786, 2646
BfaI CTAG 3 cut(s) 770, 1907, 2199
BfmI CTRYAG 3 cut(s) 1365, 2429, 2598
BfoI RGCGCY 1 cut(s) 1926
BfuAI ACCTGC 1 cut(s) 941
BfuI GTATCC 2 cut(s) 725, 891
BglII AGATCT 1 cut(s) 1911
BmcAI AGTACT 1 cut(s) 2123
Bme1390I CCNGG 6 cut(s) 110, 399, 713, 779, 2567, 2579
Bme18I GGWCC 5 cut(s) 246, 764, 1208, 1847, 2113
BmeT110I CYCGRG 1 cut(s) 2183
BmgT120I GGNCC 7 cut(s) 246, 764, 844, 1208, 1558, 1847, 2113
BmiI GGNNCC 3 cut(s) 1393, 2115, 2314
BmrFI CCNGG 6 cut(s) 110, 399, 713, 779, 2567, 2579
BmrI ACTGGG 1 cut(s) 2260
BmsI GCATC 7 cut(s) 937, 1228, 1977, 2044, 2488, 2512, 2513
BmuI ACTGGG 1 cut(s) 2260
BoxI GACNNNNGTC 1 cut(s) 779
BpiI GAAGAC 1 cut(s) 1994
BplI GAGNNNNNCTC 2 cut(s) 1216, 1248
BpmI CTGGAG 3 cut(s) 642, 1515, 2600
Bpu10I CCTNAGC 2 cut(s) 1233, 1763
BpuEI CTTGAG 2 cut(s) 870, 1425
BpuMI CCSGG 2 cut(s) 779, 2567
BsaBI GATNNNNATC 1 cut(s) 101
BsaI GGTCTC 2 cut(s) 1429, 1786
BsaJI CCNNGG 8 cut(s) 398, 621, 712, 777, 855, 1527, 1561, 2025
Bsc4I CCNNNNNNNGG 4 cut(s) 243, 673, 712, 2396
Bse1I ACTGG 5 cut(s) 698, 706, 1957, 2014, 2255
Bse3DI GCAATG 2 cut(s) 1419, 1654
Bse8I GATNNNNATC 1 cut(s) 101
BseBI CCWGG 4 cut(s) 110, 399, 713, 2579
BseDI CCNNGG 8 cut(s) 398, 621, 712, 777, 855, 1527, 1561, 2025
BseGI GGATG 4 cut(s) 952, 1243, 1300, 1998
BseJI GATNNNNATC 1 cut(s) 101
BseLI CCNNNNNNNGG 4 cut(s) 243, 673, 712, 2396
BseMI GCAATG 2 cut(s) 1419, 1654
BseMII CTCAG 3 cut(s) 1247, 1754, 2109
BseNI ACTGG 5 cut(s) 698, 706, 1957, 2014, 2255
BseRI GAGGAG 2 cut(s) 324, 2294
Bsh1236I CGCG 5 cut(s) 623, 1190, 1583, 1932, 1980
BshFI GGCC 7 cut(s) 524, 845, 1560, 1566, 2203, 2246, 2389
BsiHKAI GWGCWC 2 cut(s) 1234, 2376
BsiHKCI CYCGRG 1 cut(s) 2183
BsiSI CCGG 3 cut(s) 779, 812, 2567
BslFI GGGAC 2 cut(s) 998, 2079
BslI CCNNNNNNNGG 4 cut(s) 243, 673, 712, 2396
BsmAI GTCTC 4 cut(s) 1429, 1491, 1786, 2646
BsmFI GGGAC 2 cut(s) 998, 2079
BsmI GAATGC 2 cut(s) 2262, 2444
BsnI GGCC 7 cut(s) 524, 845, 1560, 1566, 2203, 2246, 2389
Bso31I GGTCTC 2 cut(s) 1429, 1786
BsoBI CYCGRG 1 cut(s) 2183
Bsp1286I GDGCHC 2 cut(s) 1234, 2376
Bsp1407I TGTACA 3 cut(s) 744, 1419, 1486
Bsp143I GATC 7 cut(s) 451, 594, 1017, 1818, 1858, 1911, 2617
BspANI GGCC 7 cut(s) 524, 845, 1560, 1566, 2203, 2246, 2389
BspCNI CTCAG 3 cut(s) 1246, 1755, 2110
BspFNI CGCG 5 cut(s) 623, 1190, 1583, 1932, 1980
BspHI TCATGA 1 cut(s) 477
BspLI GGNNCC 3 cut(s) 1393, 2115, 2314
BspMAI CTGCAG 2 cut(s) 1369, 2433
BspMI ACCTGC 1 cut(s) 941
BspPI GGATC 1 cut(s) 1012
BspTNI GGTCTC 2 cut(s) 1429, 1786
BsrBI CCGCTC 1 cut(s) 1358
BsrDI GCAATG 2 cut(s) 1419, 1654
BsrGI TGTACA 3 cut(s) 744, 1419, 1486
BsrI ACTGG 5 cut(s) 698, 706, 1957, 2014, 2255
BssECI CCNNGG 8 cut(s) 398, 621, 712, 777, 855, 1527, 1561, 2025
BssMI GATC 7 cut(s) 451, 594, 1017, 1818, 1858, 1911, 2617
BssT1I CCWWGG 3 cut(s) 855, 1561, 2025
Bst2UI CCWGG 4 cut(s) 110, 399, 713, 2579
Bst4CI ACNGT 5 cut(s) 801, 1126, 1133, 1890, 2378
Bst6I CTCTTC 5 cut(s) 1177, 1427, 1474, 1552, 2261
BstAPI GCANNNNNTGC 1 cut(s) 932
BstAUI TGTACA 3 cut(s) 744, 1419, 1486
BstC8I GCNNGC 4 cut(s) 1930, 1934, 2205, 2279
BstDEI CTNAG 3 cut(s) 1233, 1763, 2118
BstDSI CCRYGG 2 cut(s) 621, 1527
BstF5I GGATG 4 cut(s) 952, 1243, 1300, 1998
BstFNI CGCG 5 cut(s) 623, 1190, 1583, 1932, 1980
BstH2I RGCGCY 1 cut(s) 1926
BstHHI GCGC 5 cut(s) 937, 1192, 1925, 2059, 2198
BstKTI GATC 7 cut(s) 454, 597, 1020, 1821, 1861, 1914, 2620
BstMAI GTCTC 4 cut(s) 1429, 1491, 1786, 2646
BstMBI GATC 7 cut(s) 451, 594, 1017, 1818, 1858, 1911, 2617
BstNI CCWGG 4 cut(s) 110, 399, 713, 2579
BstNSI RCATGY 2 cut(s) 1493, 1543
BstPAI GACNNNNGTC 1 cut(s) 779
BstSCI CCNGG 6 cut(s) 108, 397, 711, 777, 2565, 2577
BstSFI CTRYAG 3 cut(s) 1365, 2429, 2598
BstUI CGCG 5 cut(s) 623, 1190, 1583, 1932, 1980
BstV2I GAAGAC 1 cut(s) 1994
BstX2I RGATCY 1 cut(s) 1911
BstXI CCANNNNNNTGG 2 cut(s) 841, 1893
BstYI RGATCY 1 cut(s) 1911
BsuI GTATCC 2 cut(s) 725, 891
BsuRI GGCC 7 cut(s) 524, 845, 1560, 1566, 2203, 2246, 2389
BtgI CCRYGG 2 cut(s) 621, 1527
BtgZI GCGATG 2 cut(s) 255, 1995
BtsCI GGATG 4 cut(s) 952, 1243, 1300, 1998
BtsI GCAGTG 2 cut(s) 2019, 2267
BtsIMutI CAGTG 4 cut(s) 705, 1886, 2019, 2267
BveI ACCTGC 1 cut(s) 941
Cac8I GCNNGC 4 cut(s) 1930, 1934, 2205, 2279
CaiI CAGNNNCTG 1 cut(s) 2140
CciI TCATGA 1 cut(s) 477
CfoI GCGC 5 cut(s) 937, 1192, 1925, 2059, 2198
Cfr13I GGNCC 7 cut(s) 246, 764, 844, 1208, 1558, 1847, 2113
Cfr42I CCGCGG 1 cut(s) 624
CseI GACGC 1 cut(s) 1196
Csp6I GTAC 9 cut(s) 490, 562, 745, 1420, 1487, 1543, 2082, 2107, 2122
CspCI CAANNNNNGTGG 2 cut(s) 627, 662
CviQI GTAC 9 cut(s) 490, 562, 745, 1420, 1487, 1543, 2082, 2107, 2122
DdeI CTNAG 3 cut(s) 1233, 1763, 2118
DpnI GATC 7 cut(s) 453, 596, 1019, 1820, 1860, 1913, 2619
DpnII GATC 7 cut(s) 451, 594, 1017, 1818, 1858, 1911, 2617
DraIII CACNNNGTG 1 cut(s) 2380
DrdI GACNNNNNNGTC 1 cut(s) 1163
DseDI GACNNNNNNGTC 1 cut(s) 1163
EaeI YGGCCR 2 cut(s) 1564, 2244
Eam1104I CTCTTC 5 cut(s) 1177, 1427, 1474, 1552, 2261
EarI CTCTTC 5 cut(s) 1177, 1427, 1474, 1552, 2261
Eco130I CCWWGG 3 cut(s) 855, 1561, 2025
Eco147I AGGCCT 2 cut(s) 2203, 2389
Eco31I GGTCTC 2 cut(s) 1429, 1786
Eco47I GGWCC 5 cut(s) 246, 764, 1208, 1847, 2113
Eco57I CTGAAG 2 cut(s) 440, 677
Eco88I CYCGRG 1 cut(s) 2183
EcoO109I RGGNCCY 2 cut(s) 246, 764
EcoRII CCWGG 4 cut(s) 108, 397, 711, 2577
EcoT14I CCWWGG 3 cut(s) 855, 1561, 2025
EcoT22I ATGCAT 1 cut(s) 1006
ErhI CCWWGG 3 cut(s) 855, 1561, 2025
FalI AAGNNNNNCTT 4 cut(s) 1176, 1208, 2084, 2116
FaqI GGGAC 2 cut(s) 998, 2079
FauI CCCGC 1 cut(s) 1925
FauNDI CATATG 2 cut(s) 939, 1006
FbaI TGATCA 1 cut(s) 2617
FblI GTMKAC 3 cut(s) 1077, 2160, 2295
FokI GGATG 4 cut(s) 959, 1250, 1307, 1985
FspBI CTAG 3 cut(s) 770, 1907, 2199
FspI TGCGCA 1 cut(s) 936
GlaI GCGC 5 cut(s) 936, 1191, 1924, 2058, 2197
GsuI CTGGAG 3 cut(s) 642, 1515, 2600
HaeII RGCGCY 1 cut(s) 1926
HaeIII GGCC 7 cut(s) 524, 845, 1560, 1566, 2203, 2246, 2389
HapII CCGG 3 cut(s) 779, 812, 2567
HgaI GACGC 1 cut(s) 1196
HhaI GCGC 5 cut(s) 937, 1192, 1925, 2059, 2198
Hin6I GCGC 5 cut(s) 935, 1190, 1923, 2057, 2196
HinP1I GCGC 5 cut(s) 935, 1190, 1923, 2057, 2196
HincII GTYRAC 3 cut(s) 1168, 2239, 2296
HindII GTYRAC 3 cut(s) 1168, 2239, 2296
HindIII AAGCTT 1 cut(s) 2098
HinfI GANTC 7 cut(s) 260, 1308, 1322, 1569, 1613, 1744, 2327
HpaII CCGG 3 cut(s) 779, 812, 2567
HphI GGTGA 4 cut(s) 172, 1967, 2357, 2440
Hpy99I CGWCG 1 cut(s) 88
HpyCH4III ACNGT 5 cut(s) 801, 1126, 1133, 1890, 2378
HpyCH4IV ACGT 4 cut(s) 83, 380, 573, 756
HpyF3I CTNAG 3 cut(s) 1233, 1763, 2118
HpySE526I ACGT 4 cut(s) 83, 380, 573, 756
HspAI GCGC 5 cut(s) 935, 1190, 1923, 2057, 2196
Ksp22I TGATCA 1 cut(s) 2617
KspI CCGCGG 1 cut(s) 624
Kzo9I GATC 7 cut(s) 451, 594, 1017, 1818, 1858, 1911, 2617
LmnI GCTCC 7 cut(s) 229, 504, 661, 913, 1355, 1391, 2542
LweI GCATC 7 cut(s) 937, 1228, 1977, 2044, 2488, 2512, 2513
MaeI CTAG 3 cut(s) 770, 1907, 2199
MaeII ACGT 4 cut(s) 83, 380, 573, 756
MaeIII GTNAC 7 cut(s) 574, 772, 976, 1010, 1116, 1476, 1587
MalI GATC 7 cut(s) 453, 596, 1019, 1820, 1860, 1913, 2619
MbiI CCGCTC 1 cut(s) 1358
MboI GATC 7 cut(s) 451, 594, 1017, 1818, 1858, 1911, 2617
MfeI CAATTG 1 cut(s) 639
MflI RGATCY 1 cut(s) 1911
MhlI GDGCHC 2 cut(s) 1234, 2376
MlyI GAGTC 2 cut(s) 1317, 1607
MmeI TCCRAC 1 cut(s) 1351
Mph1103I ATGCAT 1 cut(s) 1006
MroXI GAANNNNTTC 1 cut(s) 1463
MseI TTAA 6 cut(s) 80, 753, 828, 1284, 2478, 2613
MslI CAYNNNNRTG 3 cut(s) 1866, 1891, 1980
MspA1I CMGCKG 5 cut(s) 623, 1338, 2495, 2528, 2549
MspI CCGG 3 cut(s) 779, 812, 2567
MspR9I CCNGG 6 cut(s) 110, 399, 713, 779, 2567, 2579
MunI CAATTG 1 cut(s) 639
Mva1269I GAATGC 2 cut(s) 2262, 2444
MvaI CCWGG 4 cut(s) 110, 399, 713, 2579
MvnI CGCG 5 cut(s) 623, 1190, 1583, 1932, 1980
NciI CCSGG 2 cut(s) 779, 2567
NdeI CATATG 2 cut(s) 939, 1006
NdeII GATC 7 cut(s) 451, 594, 1017, 1818, 1858, 1911, 2617
NlaIV GGNNCC 3 cut(s) 1393, 2115, 2314
NmeAIII GCCGAG 1 cut(s) 2380
NmuCI GTSAC 4 cut(s) 574, 772, 976, 1116
NsbI TGCGCA 1 cut(s) 936
NsiI ATGCAT 1 cut(s) 1006
NspI RCATGY 2 cut(s) 1493, 1543
PaeR7I CTCGAG 1 cut(s) 2183
PagI TCATGA 1 cut(s) 477
PaqCI CACCTGC 1 cut(s) 941
PceI AGGCCT 2 cut(s) 2203, 2389
PctI GAATGC 2 cut(s) 2262, 2444
PdmI GAANNNNTTC 1 cut(s) 1463
PfeI GAWTC 5 cut(s) 260, 1322, 1569, 1744, 2327
PfoI TCCNGGA 1 cut(s) 2577
PleI GAGTC 2 cut(s) 1316, 1607
PpsI GAGTC 2 cut(s) 1316, 1607
PpuMI RGGWCCY 2 cut(s) 246, 764
PshAI GACNNNNGTC 1 cut(s) 779
PshBI ATTAAT 2 cut(s) 2478, 2613
Psp5II RGGWCCY 2 cut(s) 246, 764
Psp6I CCWGG 4 cut(s) 108, 397, 711, 2577
PspGI CCWGG 4 cut(s) 108, 397, 711, 2577
PspN4I GGNNCC 3 cut(s) 1393, 2115, 2314
PspPI GGNCC 7 cut(s) 246, 764, 844, 1208, 1558, 1847, 2113
PspPPI RGGWCCY 2 cut(s) 246, 764
PspXI VCTCGAGB 1 cut(s) 2183
PstI CTGCAG 2 cut(s) 1369, 2433
PstNI CAGNNNCTG 1 cut(s) 2140
PsuI RGATCY 1 cut(s) 1911
RsaI GTAC 9 cut(s) 491, 563, 746, 1421, 1488, 1544, 2083, 2108, 2123
RsaNI GTAC 9 cut(s) 490, 562, 745, 1420, 1487, 1543, 2082, 2107, 2122
RseI CAYNNNNRTG 3 cut(s) 1866, 1891, 1980
SacII CCGCGG 1 cut(s) 624
SalI GTCGAC 1 cut(s) 2294
SaqAI TTAA 6 cut(s) 80, 753, 828, 1284, 2478, 2613
Sau3AI GATC 7 cut(s) 451, 594, 1017, 1818, 1858, 1911, 2617
Sau96I GGNCC 7 cut(s) 246, 764, 844, 1208, 1558, 1847, 2113
ScaI AGTACT 1 cut(s) 2123
SchI GAGTC 2 cut(s) 1317, 1607
ScrFI CCNGG 6 cut(s) 110, 399, 713, 779, 2567, 2579
SduI GDGCHC 2 cut(s) 1234, 2376
SfaNI GCATC 7 cut(s) 937, 1228, 1977, 2044, 2488, 2512, 2513
SfcI CTRYAG 3 cut(s) 1365, 2429, 2598
Sfr274I CTCGAG 1 cut(s) 2183
Sfr303I CCGCGG 1 cut(s) 624
SgrBI CCGCGG 1 cut(s) 624
SinI GGWCC 5 cut(s) 246, 764, 1208, 1847, 2113
SlaI CTCGAG 1 cut(s) 2183
SmiMI CAYNNNNRTG 3 cut(s) 1866, 1891, 1980
SmlI CTYRAG 3 cut(s) 885, 1440, 2183
SmoI CTYRAG 3 cut(s) 885, 1440, 2183
SseBI AGGCCT 2 cut(s) 2203, 2389
SspMI CTAG 3 cut(s) 770, 1907, 2199
StuI AGGCCT 2 cut(s) 2203, 2389
StyD4I CCNGG 6 cut(s) 108, 397, 711, 777, 2565, 2577
StyI CCWWGG 3 cut(s) 855, 1561, 2025
TaaI ACNGT 5 cut(s) 801, 1126, 1133, 1890, 2378
TaiI ACGT 4 cut(s) 86, 383, 576, 759
TaqI TCGA 9 cut(s) 86, 204, 593, 1311, 1611, 1616, 2007, 2184, 2295
TaqII GACCGA 1 cut(s) 593
TatI WGTACW 6 cut(s) 489, 744, 1419, 1486, 1542, 2121
TauI GCSGC 4 cut(s) 527, 626, 2528, 2549
TfiI GAWTC 5 cut(s) 260, 1322, 1569, 1744, 2327
Tru1I TTAA 6 cut(s) 80, 753, 828, 1284, 2478, 2613
Tru9I TTAA 6 cut(s) 80, 753, 828, 1284, 2478, 2613
TscAI CASTG 4 cut(s) 705, 1893, 2026, 2267
TseFI GTSAC 4 cut(s) 574, 772, 976, 1116
Tsp45I GTSAC 4 cut(s) 574, 772, 976, 1116
TspDTI ATGAA 7 cut(s) 619, 1164, 1280, 1818, 1825, 1937, 2348
TspGWI ACGGA 4 cut(s) 1010, 1200, 1516, 2545
TspRI CASTG 4 cut(s) 705, 1893, 2026, 2267
VpaK11BI GGWCC 5 cut(s) 246, 764, 1208, 1847, 2113
VspI ATTAAT 2 cut(s) 2478, 2613
XapI RAATTY 1 cut(s) 725
XceI RCATGY 2 cut(s) 1493, 1543
XcmI CCANNNNNNNNNTGG 1 cut(s) 1890
XhoI CTCGAG 1 cut(s) 2183
XmiI GTMKAC 3 cut(s) 1077, 2160, 2295
XmnI GAANNNNTTC 1 cut(s) 1463
XspI CTAG 3 cut(s) 770, 1907, 2199
ZrmI AGTACT 1 cut(s) 2123
Zsp2I ATGCAT 1 cut(s) 1006
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.