MD05G1060800.v1.1

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010976
Physical Location & Seq
Reverse (-)
10529763 .. 10532438
2676 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1060800.v1.1.491

Sequence Viewer

Length: 2676 bp
ATGGAGATAGAAAAAAAACCAACCAAACAAAACCCAAACCTTTTTCTATCCTCCTTGCCATCATCATTTTCGACACCGATGGCTCTCCTCCTGGTCCTTCTCTTCTCCCTCCTTGTCTCCCCTGCCTTAGTCCTCTCTGCCTCATCACCACCCTTTACTCCCCAAGACAACTTCCTCATCGACTGTGGAGCCACCAACAAGGCCACCCTACCCGATGGGAGAACCTTCAAAACTGAATCAGAATCCACGCAGCAGTTGAAGGCAAACGATGACAACAAGGTCTCTGTTGACAAGGCAGATGTGCCTTCCCCTCTGTATTTGACTGCTAGGATTTTCAAGAGTGATGCCACTTACACGTTTCACATGGCAAGGCCAGGATGGCATTGGATTCGCCTTCATTTATACCCACTAAGCAACTCGGTGTTCGATCTTCAGAAGGCTAAGTTCAATGTCATGACAGACAAGTATACTTTACTGCACAACTTCAATGTCAATGAGAACAAGAACAACAACACCAAAAACCAAATTTTTATGAAGGAATACCTTCTAAACATAACCGAGCAGCAATTCTCCATCAAGTTCTCTCCCATGAAGAACTCTGCAGCGTTCATCAATGCGGTTGAGGTGGTTTCAGCTCCTGATAATTTGATCACCGACACAGCCAATAATCTTCAGCCGGCGAGCCAGTTTCAGGGGCTGTCCAAATTCGGTTACGAAACTATGTACAGGCTTAACATGGGAGGACCTCTTGTCACGTCCACAAATGACACATTAGGACGTACTTGGTTGCCTGATGATGCATACATTAAGTCAAAAAACATGGCCAAGGCTACATCTGTTGGGACTAGTACAGTCAAATACCCTGATGGACTAACCCCCCAAATTGCGCCACCAACTATTTATGCAACTGTTGTGCAAATGGCTGATGCTGTTGTGAGCAATCAAAATTTCAACATAACGTGGAATTTCCAGGCAGAAAACGCATTCGCATACTTAATTCGGTTACATTTCTGTGACATTGTAAGCAAATCACTTGGTAACCTCTACTTCAATGTGTACATTAATGGGAACATGGCAATAAGTGATTTGGATTTGTCACACACTGTCAACGGTTTGGCAATTCCGTACTACAAGGACATCGTCGTAAATGCTTCCTTGGTATCTAACGGATTATCAGTCCAAATTGGTCCTTCAAGAATGGACAATGGTGCGCCAAATGCCATTTTGAATGGTCTGGAAGTGATGAAACTAAGCAATTCCGTGGACAGTTTGGATGGAGAATTCGGGGTTGATGGAAAGAAAGTGGAAACAGAAGGTGGCGGGCCTCACAGAGGTGCGGTGGCTGCAGTTGGGTTTGGTTTAATGTTCGGGGCCTTCGTGGGGCTCGGAGCAATGGTTTACAAATGGCGAAAGAGACCTCAAGATTGGCAAAAGAGAAACAGTTTCTCTTCTTGGTTACTTCCTGTCCATGCCGGCGATACCAGCTTCATGTCTAGCAAGACCTCAATGGGGTCTCACAAGAGCAACATGTACAACTCCACCTTAGGCCTAGGCCGCTACTTCACTTTCCAGGAGTTACAAGAAGCCACCAACAACTTTGACTCAAGTAAGATCATTGGTGTTGGTGGATTCGGCAATGTGTATCTTGGCATAATTGATGACGGGACTAGTGTGGCTGTTAAGCGAGGAAACCCTCAATCTGAACAAGGCATTACAGAGTTCCAAACAGAAATCCAGATGTTGTCCAAGCTAAGGCATAGGCATTTGGTGTCACTGATTGGCTACTGTGACGAAAACTCAGAGATGATCTTAGTTTATGAGTACATGTCCAATGGACCTTTCCGCGATCATCTCTATGGAAAGAACTTGCCGCCATTGTCATGGAAGCAAAGACTAGAAATCTGTATTGGGTCAGCTCGAGGGCTTCATTACCTTCACACAGGGACATCACAAGGCATCATTCACCGTGACGTCAAGACAACCAACATTTTGCTTGATGATCAATTTACTGCCAAGGTGGCTGACTTCGGTCTTTCTAAGGACGCGCCTATGGGACAAAACCACGTCAGCACCGCCGTGAAAGGAAGCTTCGGGTACTTAGACCCCGAATACTTTAGAAGGCAGCAATTAACAGACAAGTCAGATGTGTACTCGTTCGGCGTTGTTTTACTTGAAGCACTGTGTGCAAGGCCTGCTATCAACCCAGCACTTCCAAGGGAGCAAGTGAACTTGGCTGACTGGGGAATGCAGTGGAAGAGGAAGGGTCTGCTCGAAAAGATCATTGATCCTCTCCTCGCCGGATCCATTAACCCGGAATCAATGAAAAAGTTTGCCGAGGCTGCAGAGAAATGTTTGGCAGAACATGGTGTGGACAGGCCTACAATGGGAGATGTGTTGTGGAACTTGGAGTATGCTTTGCAACTTCAAGAGGCATTCACTCAAGGAAAGGCTGAGGAAGAAAGTAGCAGCGCTGTGCCGCCTTCCACTGAGGCCACTACCACTGCCACATCTCCTACTGGTGCTGCTGCCGTGGTTCCTCCTCCTACTACCCCCACTTCCGCTAGCCGCCCGGCTACTATTCTGGAAGGCGACGAGGGTTCGACTGATGCTCACCCCATTAATGACCAATCAGGAACTGAAGTGTTTGCTCAATTTTCCAATCTAAATGGTAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Malectin_like Pkinase_Tyr

Protein Analysis

892

Amino Acids

97.87

Weight (kDa)

6.02

Isoelectric Point (pI)

35.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin PF11721 59 - 206 3.6e-06 Malectin domain
Malectin_like PF12819 60 - 416 9.1e-27 Malectin-like domain
Malectin PF11721 241 - 393 5.2e-09 Malectin domain
Pkinase PF00069 535 - 798 4.9e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 536 - 748 1.1e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010976)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21480 AT4G39110
fragaria_vesca FvH4_2g09700
malus_domestica MD05G1060800.v1.1 MD10G1068600.v1.1
prunus_persica Prupe.8G090600_v2.0.a1
pyrus_communis pycom10g05470
rosa_chinensis RchiOBHm_Chr6g0266011
rosa_laevigata RLG00000014135 RLG00000014136 RLG00000014138
rosa_multiflora Rmu_sc0000776.1_g000006
rosa_roxburghii Rroxscaffold_174G00435080 Rroxscaffold_7G00202100
rosa_rugosa Rorug06G0022500
rosa_samantha Rh6AG143700 Rh6BG144000 Rh6CG140700 Rh6DG128300
rosa_wichuraiana Rw6G012420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 278
AatII GACGTC 1 cut(s) 1974
AccI GTMKAC 1 cut(s) 467
AccII CGCG 2 cut(s) 1845, 2045
AclWI GGATC 3 cut(s) 2279, 2295, 2308
AcoI YGGCCR 1 cut(s) 822
AcsI RAATTY 5 cut(s) 525, 704, 946, 964, 1280
AcuI CTGAAG 3 cut(s) 416, 656, 2658
AcyI GRCGYC 1 cut(s) 1971
AdeI CACNNNGTG 1 cut(s) 2183
AfaI GTAC 9 cut(s) 725, 781, 850, 1058, 1127, 1532, 1823, 2096, 2150
AfeI AGCGCT 1 cut(s) 2470
AfiI CCNNNNNNNGG 6 cut(s) 691, 1331, 1380, 1509, 1752, 2384
AflIII ACRYGT 3 cut(s) 354, 1527, 1824
AhdI GACNNNNNGTC 1 cut(s) 749
AhlI ACTAGT 2 cut(s) 845, 1667
AjiI CACGTC 2 cut(s) 756, 2065
AjnI CCWGG 4 cut(s) 90, 373, 969, 1569
AjuI GAANNNNNNNTTGG 4 cut(s) 1823, 1855, 1890, 1922
AleI CACNNNNGTG 2 cut(s) 1332, 2075
AluBI AGCT 5 cut(s) 635, 1485, 1750, 1916, 2088
AluI AGCT 5 cut(s) 635, 1485, 1750, 1916, 2088
Alw26I GTCTC 4 cut(s) 121, 286, 1408, 1518
AlwI GGATC 3 cut(s) 2279, 2295, 2308
AlwNI CAGNNNCTG 3 cut(s) 638, 697, 2636
Ama87I CYCGRG 1 cut(s) 1917
Aor51HI AGCGCT 1 cut(s) 2470
ApeKI GCWGC 9 cut(s) 250, 562, 602, 1343, 2122, 2339, 2466, 2522, 2525
ApoI RAATTY 5 cut(s) 525, 704, 946, 964, 1280
AseI ATTAAT 2 cut(s) 1062, 2619
Asp700I GAANNNNTTC 1 cut(s) 543
AspA2I CCTAGG 1 cut(s) 1549
AspLEI GCGC 4 cut(s) 889, 1213, 2047, 2471
AspS9I GGNCC 6 cut(s) 94, 743, 1187, 1322, 1371, 1835
AsuC2I CCSGG 2 cut(s) 2312, 2570
AsuHPI GGTGA 4 cut(s) 138, 643, 1955, 2603
AsuNHI GCTAGC 1 cut(s) 2561
AvaI CYCGRG 1 cut(s) 1917
AvaII GGWCC 4 cut(s) 94, 743, 1187, 1835
AvrII CCTAGG 1 cut(s) 1549
AxyI CCTNAGG 1 cut(s) 1543
BalI TGGCCA 1 cut(s) 824
BamHI GGATCC 1 cut(s) 2300
BanII GRGCYC 1 cut(s) 1386
BbvCI CCTCAGC 1 cut(s) 2451
BbvI GCAGC 9 cut(s) 262, 574, 614, 1330, 2134, 2326, 2478, 2509, 2512
BccI CCATC 8 cut(s) 67, 73, 209, 372, 581, 860, 1268, 1286
BceAI ACGGC 2 cut(s) 2060, 2513
BcgI CGANNNNNNTGC 2 cut(s) 371, 405
BciT130I CCWGG 4 cut(s) 92, 375, 971, 1571
BclI TGATCA 2 cut(s) 648, 1999
BcnI CCSGG 2 cut(s) 2312, 2570
BcoDI GTCTC 4 cut(s) 121, 286, 1408, 1518
BcuI ACTAGT 2 cut(s) 845, 1667
BfaI CTAG 7 cut(s) 327, 846, 1494, 1550, 1668, 1895, 2562
BfmI CTRYAG 3 cut(s) 600, 1344, 2340
BfoI RGCGCY 1 cut(s) 2472
BglI GCCNNNNNGGC 2 cut(s) 379, 2018
BlnI CCTAGG 1 cut(s) 1549
Bme1390I CCNGG 6 cut(s) 92, 375, 971, 1571, 2312, 2570
Bme18I GGWCC 4 cut(s) 94, 743, 1187, 1835
BmeRI GACNNNNNGTC 1 cut(s) 749
BmeT110I CYCGRG 1 cut(s) 1917
BmgBI CACGTC 2 cut(s) 756, 2065
BmgT120I GGNCC 6 cut(s) 94, 743, 1187, 1322, 1371, 1835
BmiI GGNNCC 4 cut(s) 190, 1372, 2302, 2535
BmrFI CCNGG 6 cut(s) 92, 375, 971, 1571, 2312, 2570
BmrI ACTGGG 1 cut(s) 2248
BmsI GCATC 5 cut(s) 334, 787, 916, 1965, 2596
BmtI GCTAGC 1 cut(s) 2565
BmuI ACTGGG 1 cut(s) 2248
BoxI GACNNNNGTC 1 cut(s) 2028
Bpu10I CCTNAGC 2 cut(s) 1751, 2451
BpuEI CTTGAG 3 cut(s) 1404, 1588, 2424
BpuMI CCSGG 2 cut(s) 2312, 2570
BsaHI GRCGYC 1 cut(s) 1971
BsaI GGTCTC 3 cut(s) 286, 1408, 1518
BsaJI CCNNGG 8 cut(s) 825, 1155, 1260, 1549, 2013, 2213, 2334, 2529
BsaXI ACNNNNNCTCC 2 cut(s) 1380, 1410
Bsc4I CCNNNNNNNGG 6 cut(s) 691, 1331, 1380, 1509, 1752, 2384
Bse118I RCCGGY 2 cut(s) 676, 1472
Bse1I ACTGG 3 cut(s) 685, 2243, 2521
Bse21I CCTNAGG 1 cut(s) 1543
Bse3DI GCAATG 2 cut(s) 1398, 1642
BseBI CCWGG 4 cut(s) 92, 375, 971, 1571
BseDI CCNNGG 8 cut(s) 825, 1155, 1260, 1549, 2013, 2213, 2334, 2529
BseGI GGATG 2 cut(s) 383, 1279
BseLI CCNNNNNNNGG 6 cut(s) 691, 1331, 1380, 1509, 1752, 2384
BseMI GCAATG 2 cut(s) 1398, 1642
BseMII CTCAG 3 cut(s) 1812, 2442, 2478
BseNI ACTGG 3 cut(s) 685, 2243, 2521
BseRI GAGGAG 3 cut(s) 77, 2282, 2529
BseXI GCAGC 9 cut(s) 262, 574, 614, 1330, 2134, 2326, 2478, 2509, 2512
BseYI CCCAGC 1 cut(s) 2203
BsgI GTGCAG 1 cut(s) 461
Bsh1236I CGCG 2 cut(s) 1845, 2045
BsiHKCI CYCGRG 1 cut(s) 1917
BsiSI CCGG 5 cut(s) 677, 1473, 2298, 2312, 2570
BslFI GGGAC 4 cut(s) 856, 1678, 1957, 2067
BslI CCNNNNNNNGG 6 cut(s) 691, 1331, 1380, 1509, 1752, 2384
BsmAI GTCTC 4 cut(s) 121, 286, 1408, 1518
BsmFI GGGAC 4 cut(s) 856, 1678, 1957, 2067
BsmI GAATGC 3 cut(s) 983, 2250, 2432
Bso31I GGTCTC 3 cut(s) 286, 1408, 1518
BsoBI CYCGRG 1 cut(s) 1917
Bsp1286I GDGCHC 1 cut(s) 1386
Bsp1407I TGTACA 3 cut(s) 723, 1056, 1530
Bsp143I GATC 9 cut(s) 427, 648, 1611, 1806, 1846, 1999, 2277, 2284, 2300
BspCNI CTCAG 3 cut(s) 1811, 2443, 2479
BspFNI CGCG 2 cut(s) 1845, 2045
BspHI TCATGA 1 cut(s) 453
BspLI GGNNCC 4 cut(s) 190, 1372, 2302, 2535
BspMAI CTGCAG 3 cut(s) 604, 1348, 2344
BspOI GCTAGC 1 cut(s) 2565
BspPI GGATC 3 cut(s) 2279, 2295, 2308
BspTNI GGTCTC 3 cut(s) 286, 1408, 1518
BsrDI GCAATG 2 cut(s) 1398, 1642
BsrFI RCCGGY 2 cut(s) 676, 1472
BsrGI TGTACA 3 cut(s) 723, 1056, 1530
BsrI ACTGG 3 cut(s) 685, 2243, 2521
BssAI RCCGGY 2 cut(s) 676, 1472
BssECI CCNNGG 8 cut(s) 825, 1155, 1260, 1549, 2013, 2213, 2334, 2529
BssMI GATC 9 cut(s) 427, 648, 1611, 1806, 1846, 1999, 2277, 2284, 2300
BssNAI GTATAC 1 cut(s) 468
BssNI GRCGYC 1 cut(s) 1971
BssT1I CCWWGG 5 cut(s) 825, 1155, 1549, 2013, 2213
Bst1107I GTATAC 1 cut(s) 468
Bst2UI CCWGG 4 cut(s) 92, 375, 971, 1571
Bst6I CTCTTC 3 cut(s) 107, 1453, 2249
BstACI GRCGYC 1 cut(s) 1971
BstAPI GCANNNNNTGC 2 cut(s) 2183, 2192
BstAUI TGTACA 3 cut(s) 723, 1056, 1530
BstC8I GCNNGC 6 cut(s) 678, 682, 1322, 1474, 2193, 2563
BstDSI CCRYGG 2 cut(s) 1260, 2529
BstEII GGTNACC 1 cut(s) 1037
BstENI CCTNNNNNAGG 1 cut(s) 1329
BstF5I GGATG 2 cut(s) 383, 1279
BstFNI CGCG 2 cut(s) 1845, 2045
BstH2I RGCGCY 1 cut(s) 2472
BstHHI GCGC 4 cut(s) 889, 1213, 2047, 2471
BstKTI GATC 9 cut(s) 430, 651, 1614, 1809, 1849, 2002, 2280, 2287, 2303
BstMAI GTCTC 4 cut(s) 121, 286, 1408, 1518
BstMBI GATC 9 cut(s) 427, 648, 1611, 1806, 1846, 1999, 2277, 2284, 2300
BstNI CCWGG 4 cut(s) 92, 375, 971, 1571
BstNSI RCATGY 2 cut(s) 1531, 1828
BstPAI GACNNNNGTC 1 cut(s) 2028
BstPI GGTNACC 1 cut(s) 1037
BstSCI CCNGG 6 cut(s) 90, 373, 969, 1569, 2310, 2568
BstSFI CTRYAG 3 cut(s) 600, 1344, 2340
BstUI CGCG 2 cut(s) 1845, 2045
BstV1I GCAGC 9 cut(s) 262, 574, 614, 1330, 2134, 2326, 2478, 2509, 2512
BstX2I RGATCY 1 cut(s) 2300
BstXI CCANNNNNNTGG 1 cut(s) 1881
BstYI RGATCY 1 cut(s) 2300
BstZ17I GTATAC 1 cut(s) 468
Bsu36I CCTNAGG 1 cut(s) 1543
BtgI CCRYGG 2 cut(s) 1260, 2529
BtrI CACGTC 2 cut(s) 756, 2065
BtsCI GGATG 2 cut(s) 383, 1279
BtsI GCAGTG 2 cut(s) 2255, 2499
BtsIMutI CAGTG 6 cut(s) 1101, 1772, 2177, 2255, 2484, 2499
Cac8I GCNNGC 6 cut(s) 678, 682, 1322, 1474, 2193, 2563
CaiI CAGNNNCTG 3 cut(s) 638, 697, 2636
CciI TCATGA 1 cut(s) 453
CfoI GCGC 4 cut(s) 889, 1213, 2047, 2471
Cfr10I RCCGGY 2 cut(s) 676, 1472
Cfr13I GGNCC 6 cut(s) 94, 743, 1187, 1322, 1371, 1835
CseI GACGC 1 cut(s) 2051
Csp6I GTAC 9 cut(s) 724, 780, 849, 1057, 1126, 1531, 1822, 2095, 2149
CviQI GTAC 9 cut(s) 724, 780, 849, 1057, 1126, 1531, 1822, 2095, 2149
DpnI GATC 9 cut(s) 429, 650, 1613, 1808, 1848, 2001, 2279, 2286, 2302
DpnII GATC 9 cut(s) 427, 648, 1611, 1806, 1846, 1999, 2277, 2284, 2300
DraIII CACNNNGTG 1 cut(s) 2183
DrdI GACNNNNNNGTC 1 cut(s) 278
DriI GACNNNNNGTC 1 cut(s) 749
DseDI GACNNNNNNGTC 1 cut(s) 278
EaeI YGGCCR 1 cut(s) 822
Eam1104I CTCTTC 3 cut(s) 107, 1453, 2249
Eam1105I GACNNNNNGTC 1 cut(s) 749
EarI CTCTTC 3 cut(s) 107, 1453, 2249
Eco130I CCWWGG 5 cut(s) 825, 1155, 1549, 2013, 2213
Eco147I AGGCCT 3 cut(s) 1548, 2191, 2377
Eco24I GRGCYC 1 cut(s) 1386
Eco31I GGTCTC 3 cut(s) 286, 1408, 1518
Eco47I GGWCC 4 cut(s) 94, 743, 1187, 1835
Eco47III AGCGCT 1 cut(s) 2470
Eco57I CTGAAG 3 cut(s) 416, 656, 2658
Eco81I CCTNAGG 1 cut(s) 1543
Eco88I CYCGRG 1 cut(s) 1917
Eco91I GGTNACC 1 cut(s) 1037
EcoNI CCTNNNNNAGG 1 cut(s) 1329
EcoO109I RGGNCCY 2 cut(s) 743, 1371
EcoO65I GGTNACC 1 cut(s) 1037
EcoRI GAATTC 1 cut(s) 1280
EcoRII CCWGG 4 cut(s) 90, 373, 969, 1569
EcoT14I CCWWGG 5 cut(s) 825, 1155, 1549, 2013, 2213
EcoT22I ATGCAT 1 cut(s) 802
EcoT38I GRGCYC 1 cut(s) 1386
ErhI CCWWGG 5 cut(s) 825, 1155, 1549, 2013, 2213
FaqI GGGAC 4 cut(s) 856, 1678, 1957, 2067
FauI CCCGC 1 cut(s) 1313
FbaI TGATCA 2 cut(s) 648, 1999
FblI GTMKAC 1 cut(s) 467
FokI GGATG 2 cut(s) 390, 1286
FriOI GRGCYC 1 cut(s) 1386
FspBI CTAG 7 cut(s) 327, 846, 1494, 1550, 1668, 1895, 2562
GlaI GCGC 4 cut(s) 888, 1212, 2046, 2470
GsaI CCCAGC 1 cut(s) 2207
HaeII RGCGCY 1 cut(s) 2472
HapII CCGG 5 cut(s) 677, 1473, 2298, 2312, 2570
HgaI GACGC 1 cut(s) 2051
HhaI GCGC 4 cut(s) 889, 1213, 2047, 2471
Hin1I GRCGYC 1 cut(s) 1971
Hin6I GCGC 4 cut(s) 887, 1211, 2045, 2469
HinP1I GCGC 4 cut(s) 887, 1211, 2045, 2469
HincII GTYRAC 2 cut(s) 289, 1108
HindII GTYRAC 2 cut(s) 289, 1108
HindIII AAGCTT 1 cut(s) 2086
HinfI GANTC 6 cut(s) 236, 242, 388, 1601, 1629, 2315
HpaII CCGG 5 cut(s) 677, 1473, 2298, 2312, 2570
HphI GGTGA 4 cut(s) 138, 643, 1955, 2603
Hpy188I TCNGA 6 cut(s) 241, 435, 1388, 1702, 1801, 2143
Hpy99I CGWCG 2 cut(s) 1145, 2594
HpyCH4IV ACGT 6 cut(s) 356, 755, 778, 959, 1971, 2064
HpySE526I ACGT 6 cut(s) 356, 755, 778, 959, 1971, 2064
Hsp92I GRCGYC 1 cut(s) 1971
HspAI GCGC 4 cut(s) 887, 1211, 2045, 2469
KroI GCCGGC 2 cut(s) 676, 1472
KroNI GCCGGC 2 cut(s) 678, 1474
Ksp22I TGATCA 2 cut(s) 648, 1999
Kzo9I GATC 9 cut(s) 427, 648, 1611, 1806, 1846, 1999, 2277, 2284, 2300
LmnI GCTCC 4 cut(s) 188, 640, 1388, 2218
Lsp1109I GCAGC 9 cut(s) 262, 574, 614, 1330, 2134, 2326, 2478, 2509, 2512
LweI GCATC 5 cut(s) 334, 787, 916, 1965, 2596
MaeI CTAG 7 cut(s) 327, 846, 1494, 1550, 1668, 1895, 2562
MaeII ACGT 6 cut(s) 356, 755, 778, 959, 1971, 2064
MalI GATC 9 cut(s) 429, 650, 1613, 1808, 1848, 2001, 2279, 2286, 2302
MboI GATC 9 cut(s) 427, 648, 1611, 1806, 1846, 1999, 2277, 2284, 2300
MboII GAAGA 7 cut(s) 94, 422, 604, 662, 1440, 2266, 2468
MflI RGATCY 1 cut(s) 2300
MhlI GDGCHC 1 cut(s) 1386
MlsI TGGCCA 1 cut(s) 824
MluNI TGGCCA 1 cut(s) 824
MlyI GAGTC 1 cut(s) 1595
Mox20I TGGCCA 1 cut(s) 824
Mph1103I ATGCAT 1 cut(s) 802
MroNI GCCGGC 2 cut(s) 676, 1472
MroXI GAANNNNTTC 1 cut(s) 543
MscI TGGCCA 1 cut(s) 824
MseI TTAA 9 cut(s) 732, 807, 995, 1062, 1361, 1680, 2129, 2307, 2619
MslI CAYNNNNRTG 5 cut(s) 1011, 1332, 1854, 1879, 2075
Msp20I TGGCCA 1 cut(s) 824
MspI CCGG 5 cut(s) 677, 1473, 2298, 2312, 2570
MspR9I CCNGG 6 cut(s) 92, 375, 971, 1571, 2312, 2570
Mva1269I GAATGC 3 cut(s) 983, 2250, 2432
MvaI CCWGG 4 cut(s) 92, 375, 971, 1571
MvnI CGCG 2 cut(s) 1845, 2045
NaeI GCCGGC 2 cut(s) 678, 1474
NciI CCSGG 2 cut(s) 2312, 2570
NdeII GATC 9 cut(s) 427, 648, 1611, 1806, 1846, 1999, 2277, 2284, 2300
NgoMIV GCCGGC 2 cut(s) 676, 1472
NheI GCTAGC 1 cut(s) 2561
NlaIV GGNNCC 4 cut(s) 190, 1372, 2302, 2535
NmeAIII GCCGAG 1 cut(s) 2359
NmuCI GTSAC 6 cut(s) 751, 1013, 1095, 1770, 1787, 1967
NsiI ATGCAT 1 cut(s) 802
NspI RCATGY 2 cut(s) 1531, 1828
OliI CACNNNNGTG 2 cut(s) 1332, 2075
PaeR7I CTCGAG 1 cut(s) 1917
PagI TCATGA 1 cut(s) 453
PceI AGGCCT 3 cut(s) 1548, 2191, 2377
PciI ACATGT 2 cut(s) 1527, 1824
PcsI WCGNNNNNNNCGW 1 cut(s) 1374
PctI GAATGC 3 cut(s) 983, 2250, 2432
PdiI GCCGGC 2 cut(s) 678, 1474
PdmI GAANNNNTTC 1 cut(s) 543
PfeI GAWTC 5 cut(s) 236, 242, 388, 1629, 2315
PflFI GACNNNGTC 1 cut(s) 1139
PfoI TCCNGGA 1 cut(s) 1569
PleI GAGTC 1 cut(s) 1595
PpsI GAGTC 1 cut(s) 1595
PpuMI RGGWCCY 1 cut(s) 743
PscI ACATGT 2 cut(s) 1527, 1824
PshAI GACNNNNGTC 1 cut(s) 2028
PshBI ATTAAT 2 cut(s) 1062, 2619
Psp5II RGGWCCY 1 cut(s) 743
Psp6I CCWGG 4 cut(s) 90, 373, 969, 1569
PspEI GGTNACC 1 cut(s) 1037
PspFI CCCAGC 1 cut(s) 2203
PspGI CCWGG 4 cut(s) 90, 373, 969, 1569
PspN4I GGNNCC 4 cut(s) 190, 1372, 2302, 2535
PspPI GGNCC 6 cut(s) 94, 743, 1187, 1322, 1371, 1835
PspPPI RGGWCCY 1 cut(s) 743
PspXI VCTCGAGB 1 cut(s) 1917
PstI CTGCAG 3 cut(s) 604, 1348, 2344
PstNI CAGNNNCTG 3 cut(s) 638, 697, 2636
PsuI RGATCY 1 cut(s) 2300
PsyI GACNNNGTC 1 cut(s) 1139
RsaI GTAC 9 cut(s) 725, 781, 850, 1058, 1127, 1532, 1823, 2096, 2150
RsaNI GTAC 9 cut(s) 724, 780, 849, 1057, 1126, 1531, 1822, 2095, 2149
RseI CAYNNNNRTG 5 cut(s) 1011, 1332, 1854, 1879, 2075
SaqAI TTAA 9 cut(s) 732, 807, 995, 1062, 1361, 1680, 2129, 2307, 2619
Sau3AI GATC 9 cut(s) 427, 648, 1611, 1806, 1846, 1999, 2277, 2284, 2300
Sau96I GGNCC 6 cut(s) 94, 743, 1187, 1322, 1371, 1835
SchI GAGTC 1 cut(s) 1595
ScrFI CCNGG 6 cut(s) 92, 375, 971, 1571, 2312, 2570
SduI GDGCHC 1 cut(s) 1386
SfaNI GCATC 5 cut(s) 334, 787, 916, 1965, 2596
SfcI CTRYAG 3 cut(s) 600, 1344, 2340
Sfr274I CTCGAG 1 cut(s) 1917
SinI GGWCC 4 cut(s) 94, 743, 1187, 1835
SlaI CTCGAG 1 cut(s) 1917
SmiMI CAYNNNNRTG 5 cut(s) 1011, 1332, 1854, 1879, 2075
SmlI CTYRAG 4 cut(s) 1419, 1603, 1917, 2439
SmoI CTYRAG 4 cut(s) 1419, 1603, 1917, 2439
SpeI ACTAGT 2 cut(s) 845, 1667
SseBI AGGCCT 3 cut(s) 1548, 2191, 2377
SspMI CTAG 7 cut(s) 327, 846, 1494, 1550, 1668, 1895, 2562
StuI AGGCCT 3 cut(s) 1548, 2191, 2377
StyD4I CCNGG 6 cut(s) 90, 373, 969, 1569, 2310, 2568
StyI CCWWGG 5 cut(s) 825, 1155, 1549, 2013, 2213
TaiI ACGT 6 cut(s) 359, 758, 781, 962, 1974, 2067
TaqI TCGA 6 cut(s) 71, 180, 426, 1918, 2271, 2600
TaqII GACCGA 1 cut(s) 2018
TatI WGTACW 6 cut(s) 723, 848, 1056, 1530, 1821, 2148
TauI GCSGC 4 cut(s) 1557, 1873, 2479, 2568
TfiI GAWTC 5 cut(s) 236, 242, 388, 1629, 2315
Tru1I TTAA 9 cut(s) 732, 807, 995, 1062, 1361, 1680, 2129, 2307, 2619
Tru9I TTAA 9 cut(s) 732, 807, 995, 1062, 1361, 1680, 2129, 2307, 2619
TscAI CASTG 6 cut(s) 1108, 1779, 2184, 2255, 2491, 2506
TseFI GTSAC 6 cut(s) 751, 1013, 1095, 1770, 1787, 1967
TseI GCWGC 9 cut(s) 250, 562, 602, 1343, 2122, 2339, 2466, 2522, 2525
Tsp45I GTSAC 6 cut(s) 751, 1013, 1095, 1770, 1787, 1967
TspDTI ATGAA 8 cut(s) 386, 548, 598, 605, 1259, 1477, 1916, 2336
TspGWI ACGGA 3 cut(s) 1113, 1182, 1249
TspRI CASTG 6 cut(s) 1108, 1779, 2184, 2255, 2491, 2506
Tth111I GACNNNGTC 1 cut(s) 1139
VpaK11BI GGWCC 4 cut(s) 94, 743, 1187, 1835
VspI ATTAAT 2 cut(s) 1062, 2619
XagI CCTNNNNNAGG 1 cut(s) 1329
XapI RAATTY 5 cut(s) 525, 704, 946, 964, 1280
XceI RCATGY 2 cut(s) 1531, 1828
XcmI CCANNNNNNNNNTGG 1 cut(s) 381
XhoI CTCGAG 1 cut(s) 1917
XmaJI CCTAGG 1 cut(s) 1549
XmiI GTMKAC 1 cut(s) 467
XmnI GAANNNNTTC 1 cut(s) 543
XspI CTAG 7 cut(s) 327, 846, 1494, 1550, 1668, 1895, 2562
ZraI GACGTC 1 cut(s) 1972
Zsp2I ATGCAT 1 cut(s) 802
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.