MD10G1068600.v1.1

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010976
Physical Location & Seq
Reverse (-)
9433203 .. 9435779
2577 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1068600.v1.1.491

Sequence Viewer

Length: 2577 bp
ATGGCTCTCCTCCTGGTCCTTCTTTTCTCCCTCCTTGCCTCCCCCACCTTCGTCCTCTCAGCGTCCTCCCCACCGTTTACTCCCCAAGACAACTTCCTCATCGATTGTGGCGCCGCCACCAACAAGGCCACCCTCCCTGATGGGAGAGCCTTCAAAACTGAATCAGAATCCACGCAGCAGTTGAAGGCAGATGATGACAACAAGGTCTCTGTCGACAAGGCAGACGTGCCTTCCCCTCTTTATTTGACTGCTAGGATTTTCAAGAGCGATGCCACTTACACGTTCCACATGGCAAGGCCGGGATGGCATTGGATTCGCCTTCATTTCTACCCGCTAAGCAACTCGGTGTTCGATCTTCAAAAGGCTACATTCAATGTCATGACGCAGAAGTACACTTTGCTACACAGCTTCAATGTGAACGAAAACAAGAACAACACAAAGAACCAAATTTTTATGAAGGAGTACCTTTTGAACATAACCGAGCAGCAATTCTCCATCAAGTTCTCTCCCATGAAAAACTCTGCGGCGTTCATCAATGCCATTGAGGTGGTTTCAGCTCCTGATAATTTGATCACCGACACGGCTAACAACCTTCAGCCGGCAAGCCAATTTCAAGGGTTGTCTAAATTCGGGTATGAAACAATGTACAGGCTTAACATGGGAGGACCCCTTGTCACATCCACAAATGACACATTAGGACGCTCTTGGTTGCCCGATGACGCATACATCAAGTCCAAAAACATGGCCAAGGCTACGTCGGTTGGGACTAGTACGATCAAGTATCCCGATGGACTAACCCCCCAAATCGCACCACCAACTATTTATGCAACCGCTGTCCAAATGGCTGATGCTGTTGTGAGCAATCAAAATTTCAACATAACATGGAATTTCCAGGCGGAAAATGCATTTGCCTACTTAATTCGATTACATTTTTGTGACATTGTAAGCAAGTCACTTGGTAACCTCTACTTCAATGTGTACATTAACGGGAACATGGCGATAAGCGATTTGGATTTGTCACACACTGTTAATGGTTTGGCAATTCCATACTACAAGGACATTGTCGTAAATGCTTCATTGGTATCTGACGGGTTATCAGTCCAGATTGGTCCTTCAAGAATGGACAATGGCGCACCAAATGCCATTTTGAATGGTTTGGAGGTGATGAAACTAAGCAATTCCGTGGACAGTTTGGATGGCGAATTCGGGGTTGATGGAAAGAAAGTGGAAACAGGCAGCGGCGGGCCTCACAGAGGTGCGGTGGCTGCAGTCGGGTTTGGTTTAATGTTCGGGGCCTTCGTAGGGCTCGGAGCAATGGTTTACAAATGGCGGAAGAGACCTCAAGATTGGCAAAAGAGGAACAGTTTCTCTTCTTGGCTACTTCCTGTCCATGCCGCCGACACTAGCTTCATGTCGAGCAAGACCTCAAACAACATGTACAACTCCACATTAGGCCTTGGCCGCTACTTCACTTTTCAAGAGTTACAAGAAGCCACCAACAACTTTGACTCGAGTAAGATCATTGGTGTTGGTGGATTTGGCAATGTGTATCTTTGCGTGATTGATGATGGGACTAGCGTGGCTGTCAAGCGAGGCAACCCCCAATCCGAACAAGGCATTACAGAGTTTCAAACAGAGATCCAGATGTTGTCCAAGTTAAGGCACAGACATTTGGTGTCACTGATTGGCTACTGTGACGAAAACTCAGAGATGATCTTGGTTTATGAGTACATGTCTAATGGACCTTTCCGCGATCATCTATATGGCAAGGACTTGCCTCCGTTGTCATGGAAGCAAAGACTAGAAATCTGTATCGGGGCAGCTCGCGGGCTTCATTACCTTCACACTGGAACAACACAAGGCATCATTCATCGCGACGTTAAAACAACTAACATTTTGCTTGATGATCAATTCACTGCCAAGGTTGCTGATTTCGGTCTTTCCAAAGACGCGACGATGGGACAGAACTATGTAAGCACTGCCGTGAAAGGAAGCTTCGGGTACTTAGACCCTGAATATTTCAGAAGGCAGCAATTGACAGACAAGTCGGATGTGTACTCGTTTGGGGTTGTTCTACTTGAAGCACTGTGTGCAAGGCCTGCTATCGACCCGGCACTTCCGAGGGAGCAAGTGAACTTGGCTGACTGGGGAATGCAGTGGAAGAGGAAGGGACTTCTTGAAAAGATCATTGATCCTCTCCTCGTTGGATCCATTAACCCTGAATCAATGAAGAAGTTTGGCGAGGCTGCAGAGAAGTGTTTGGAAGAACATGGTGTGGATAGGCCCACAATGGGAGATGTGTTGTGGAACTTGGAGTATGCTCTGCAACTTCAAGAGGCATTCACTCAAGGAAAGCCTGAGGATGAAAGTAGCAGCGCTGTGCTGCCTTCCACTGAGGTCAATGCCGCTGCGACATCCCCTGCTGCTGTTGCGGTTCTTCCCCCTAATACCCCAACTTCGGCTAGCCACCCAACTACTATCCCTGAAGGCGACGAGGGTTCAACTAATGCTCACCCCATTGATGACCAATCGAGAACTGATATATTGGCTCAATTTTCCAATCTAAATGGTAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Malectin_like Pkinase_Tyr

Protein Analysis

859

Amino Acids

94.44

Weight (kDa)

5.59

Isoelectric Point (pI)

33.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin PF11721 33 - 180 2.9e-07 Malectin domain
Malectin_like PF12819 34 - 390 1.4e-28 Malectin-like domain
Malectin PF11721 215 - 366 2.7e-09 Malectin domain
Pkinase PF00069 504 - 765 1.2e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 505 - 697 2.3e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010976)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21480 AT4G39110
fragaria_vesca FvH4_2g09700
malus_domestica MD05G1060800.v1.1 MD10G1068600.v1.1
prunus_persica Prupe.8G090600_v2.0.a1
pyrus_communis pycom10g05470
rosa_chinensis RchiOBHm_Chr6g0266011
rosa_laevigata RLG00000014135 RLG00000014136 RLG00000014138
rosa_multiflora Rmu_sc0000776.1_g000006
rosa_roxburghii Rroxscaffold_174G00435080 Rroxscaffold_7G00202100
rosa_rugosa Rorug06G0022500
rosa_samantha Rh6AG143700 Rh6BG144000 Rh6CG140700 Rh6DG128300
rosa_wichuraiana Rw6G012420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 203, 2044
AccB1I GGYRCC 1 cut(s) 110
AccI GTMKAC 1 cut(s) 213
AccII CGCG 4 cut(s) 1752, 1827, 1875, 1952
AclWI GGATC 4 cut(s) 1633, 2186, 2202, 2215
AcoI YGGCCR 2 cut(s) 744, 1459
AcsI RAATTY 5 cut(s) 447, 626, 868, 886, 1202
AcuI CTGAAG 2 cut(s) 578, 2505
AcyI GRCGYC 1 cut(s) 111
AdeI CACNNNGTG 1 cut(s) 2090
AfaI GTAC 9 cut(s) 392, 464, 647, 772, 980, 1439, 1730, 2003, 2057
AfeI AGCGCT 1 cut(s) 2377
AfiI CCNNNNNNNGG 6 cut(s) 598, 1253, 1302, 1659, 2291, 2458
AflIII ACRYGT 3 cut(s) 279, 1434, 1731
AhdI GACNNNNNGTC 1 cut(s) 671
AhlI ACTAGT 1 cut(s) 767
AjiI CACGTC 1 cut(s) 226
AjnI CCWGG 2 cut(s) 12, 891
AleI CACNNNNGTG 2 cut(s) 1254, 1982
AluBI AGCT 5 cut(s) 408, 557, 1407, 1823, 1995
AluI AGCT 5 cut(s) 408, 557, 1407, 1823, 1995
Alw26I GTCTC 2 cut(s) 211, 1330
AlwI GGATC 4 cut(s) 1633, 2186, 2202, 2215
AlwNI CAGNNNCTG 1 cut(s) 560
Ama87I CYCGRG 1 cut(s) 1510
Aor51HI AGCGCT 1 cut(s) 2377
AoxI GGCC 9 cut(s) 126, 296, 744, 1244, 1293, 1453, 1459, 2096, 2282
ApoI RAATTY 5 cut(s) 447, 626, 868, 886, 1202
Asp700I GAANNNNTTC 1 cut(s) 1364
AspLEI GCGC 3 cut(s) 113, 1133, 2378
AspS9I GGNCC 7 cut(s) 16, 665, 1109, 1244, 1293, 1742, 2283
AsuC2I CCSGG 2 cut(s) 300, 2111
AsuHPI GGTGA 3 cut(s) 565, 1174, 2504
AsuNHI GCTAGC 1 cut(s) 2462
AvaI CYCGRG 1 cut(s) 1510
AvaII GGWCC 4 cut(s) 16, 665, 1109, 1742
AxyI CCTNAGG 1 cut(s) 2358
BalI TGGCCA 1 cut(s) 746
BamHI GGATCC 1 cut(s) 2207
BanI GGYRCC 1 cut(s) 110
BanII GRGCYC 1 cut(s) 1308
BccI CCATC 8 cut(s) 134, 297, 503, 782, 1190, 1208, 1562, 1951
BceAI ACGGC 2 cut(s) 597, 1967
BcgI CGANNNNNNTGC 2 cut(s) 296, 330
BciT130I CCWGG 2 cut(s) 14, 893
BciVI GTATCC 1 cut(s) 792
BclI TGATCA 2 cut(s) 570, 1906
BcnI CCSGG 2 cut(s) 300, 2111
BcoDI GTCTC 2 cut(s) 211, 1330
BcuI ACTAGT 1 cut(s) 767
BfaI CTAG 6 cut(s) 252, 768, 1404, 1575, 1802, 2463
BfmI CTRYAG 2 cut(s) 1266, 2247
BfoI RGCGCY 2 cut(s) 114, 2379
BfuI GTATCC 1 cut(s) 792
BglI GCCNNNNNGGC 1 cut(s) 304
BlpI GCTNAGC 1 cut(s) 335
Bme1390I CCNGG 4 cut(s) 14, 300, 893, 2111
Bme18I GGWCC 4 cut(s) 16, 665, 1109, 1742
BmeRI GACNNNNNGTC 1 cut(s) 671
BmeT110I CYCGRG 1 cut(s) 1510
BmgBI CACGTC 1 cut(s) 226
BmgT120I GGNCC 7 cut(s) 16, 665, 1109, 1244, 1293, 1742, 2283
BmiI GGNNCC 4 cut(s) 112, 667, 1294, 2209
BmrFI CCNGG 4 cut(s) 14, 300, 893, 2111
BmrI ACTGGG 1 cut(s) 2155
BmsI GCATC 3 cut(s) 259, 838, 1872
BmtI GCTAGC 1 cut(s) 2466
BmuI ACTGGG 1 cut(s) 2155
Bpu1102I GCTNAGC 1 cut(s) 335
BpuEI CTTGAG 2 cut(s) 1326, 2331
BpuMI CCSGG 2 cut(s) 300, 2111
Bsa29I ATCGAT 1 cut(s) 102
BsaHI GRCGYC 1 cut(s) 111
BsaI GGTCTC 2 cut(s) 211, 1330
BsaJI CCNNGG 5 cut(s) 747, 1182, 1456, 1920, 2120
BsaXI ACNNNNNCTCC 2 cut(s) 1302, 1332
Bsc4I CCNNNNNNNGG 6 cut(s) 598, 1253, 1302, 1659, 2291, 2458
Bse118I RCCGGY 1 cut(s) 598
Bse1I ACTGG 2 cut(s) 1852, 2150
Bse21I CCTNAGG 1 cut(s) 2358
Bse3DI GCAATG 2 cut(s) 1320, 1549
BseBI CCWGG 2 cut(s) 14, 893
BseCI ATCGAT 1 cut(s) 102
BseDI CCNNGG 5 cut(s) 747, 1182, 1456, 1920, 2120
BseGI GGATG 6 cut(s) 308, 677, 1201, 2056, 2368, 2414
BseLI CCNNNNNNNGG 6 cut(s) 598, 1253, 1302, 1659, 2291, 2458
BseMI GCAATG 2 cut(s) 1320, 1549
BseMII CTCAG 4 cut(s) 72, 1719, 2349, 2385
BseNI ACTGG 2 cut(s) 1852, 2150
BseRI GAGGAG 1 cut(s) 2189
Bsh1236I CGCG 4 cut(s) 1752, 1827, 1875, 1952
BshFI GGCC 9 cut(s) 128, 298, 746, 1246, 1295, 1455, 1461, 2098, 2284
BshNI GGYRCC 1 cut(s) 110
BshVI ATCGAT 1 cut(s) 102
BsiHKCI CYCGRG 1 cut(s) 1510
BsiSI CCGG 3 cut(s) 299, 599, 2111
BslFI GGGAC 4 cut(s) 778, 1585, 1974, 2184
BslI CCNNNNNNNGG 6 cut(s) 598, 1253, 1302, 1659, 2291, 2458
BsmAI GTCTC 2 cut(s) 211, 1330
BsmFI GGGAC 4 cut(s) 778, 1585, 1974, 2184
BsmI GAATGC 2 cut(s) 2157, 2339
BsnI GGCC 9 cut(s) 128, 298, 746, 1246, 1295, 1455, 1461, 2098, 2284
Bso31I GGTCTC 2 cut(s) 211, 1330
BsoBI CYCGRG 1 cut(s) 1510
Bsp1286I GDGCHC 1 cut(s) 1308
Bsp1407I TGTACA 3 cut(s) 645, 978, 1437
Bsp1720I GCTNAGC 1 cut(s) 335
Bsp68I TCGCGA 1 cut(s) 1875
BspANI GGCC 9 cut(s) 128, 298, 746, 1246, 1295, 1455, 1461, 2098, 2284
BspCNI CTCAG 4 cut(s) 71, 1718, 2350, 2386
BspDI ATCGAT 1 cut(s) 102
BspFNI CGCG 4 cut(s) 1752, 1827, 1875, 1952
BspHI TCATGA 1 cut(s) 378
BspLI GGNNCC 4 cut(s) 112, 667, 1294, 2209
BspMAI CTGCAG 2 cut(s) 1270, 2251
BspOI GCTAGC 1 cut(s) 2466
BspPI GGATC 4 cut(s) 1633, 2186, 2202, 2215
BspT107I GGYRCC 1 cut(s) 110
BspTNI GGTCTC 2 cut(s) 211, 1330
BsrDI GCAATG 2 cut(s) 1320, 1549
BsrFI RCCGGY 1 cut(s) 598
BsrGI TGTACA 3 cut(s) 645, 978, 1437
BsrI ACTGG 2 cut(s) 1852, 2150
BssAI RCCGGY 1 cut(s) 598
BssECI CCNNGG 5 cut(s) 747, 1182, 1456, 1920, 2120
BssNI GRCGYC 1 cut(s) 111
BssT1I CCWWGG 3 cut(s) 747, 1456, 1920
Bst2UI CCWGG 2 cut(s) 14, 893
Bst4CI ACNGT 6 cut(s) 75, 1027, 1190, 1364, 1694, 2088
Bst6I CTCTTC 3 cut(s) 1328, 1375, 2156
BstACI GRCGYC 1 cut(s) 111
BstAPI GCANNNNNTGC 3 cut(s) 1139, 2090, 2099
BstAUI TGTACA 3 cut(s) 645, 978, 1437
BstC8I GCNNGC 7 cut(s) 600, 604, 1244, 1825, 1829, 2100, 2464
BstDEI CTNAG 7 cut(s) 58, 335, 1172, 1705, 2005, 2358, 2394
BstDSI CCRYGG 1 cut(s) 1182
BstEII GGTNACC 1 cut(s) 959
BstENI CCTNNNNNAGG 1 cut(s) 1251
BstF5I GGATG 6 cut(s) 308, 677, 1201, 2056, 2368, 2414
BstFNI CGCG 4 cut(s) 1752, 1827, 1875, 1952
BstH2I RGCGCY 2 cut(s) 114, 2379
BstHHI GCGC 3 cut(s) 113, 1133, 2378
BstMAI GTCTC 2 cut(s) 211, 1330
BstMWI GCNNNNNNNGC 9 cut(s) 304, 902, 1139, 1265, 1461, 1925, 2090, 2099, 2429
BstNI CCWGG 2 cut(s) 14, 893
BstNSI RCATGY 2 cut(s) 1438, 1735
BstPI GGTNACC 1 cut(s) 959
BstSCI CCNGG 4 cut(s) 12, 298, 891, 2109
BstSFI CTRYAG 2 cut(s) 1266, 2247
BstUI CGCG 4 cut(s) 1752, 1827, 1875, 1952
BstX2I RGATCY 2 cut(s) 1638, 2207
BstXI CCANNNNNNTGG 2 cut(s) 547, 742
BstYI RGATCY 2 cut(s) 1638, 2207
Bsu15I ATCGAT 1 cut(s) 102
Bsu36I CCTNAGG 1 cut(s) 2358
BsuI GTATCC 1 cut(s) 792
BsuRI GGCC 9 cut(s) 128, 298, 746, 1246, 1295, 1455, 1461, 2098, 2284
BsuTUI ATCGAT 1 cut(s) 102
BtgI CCRYGG 1 cut(s) 1182
BtgZI GCGATG 2 cut(s) 282, 1856
BtrI CACGTC 1 cut(s) 226
BtsCI GGATG 6 cut(s) 308, 677, 1201, 2056, 2368, 2414
BtsI GCAGTG 3 cut(s) 1914, 1977, 2162
BtsIMutI CAGTG 8 cut(s) 1023, 1679, 1845, 1914, 1977, 2084, 2162, 2391
BtuMI TCGCGA 1 cut(s) 1875
Cac8I GCNNGC 7 cut(s) 600, 604, 1244, 1825, 1829, 2100, 2464
CaiI CAGNNNCTG 1 cut(s) 560
CciI TCATGA 1 cut(s) 378
CfoI GCGC 3 cut(s) 113, 1133, 2378
Cfr10I RCCGGY 1 cut(s) 598
Cfr13I GGNCC 7 cut(s) 16, 665, 1109, 1244, 1293, 1742, 2283
ClaI ATCGAT 1 cut(s) 102
CseI GACGC 5 cut(s) 51, 391, 708, 728, 1958
Csp6I GTAC 9 cut(s) 391, 463, 646, 771, 979, 1438, 1729, 2002, 2056
CviQI GTAC 9 cut(s) 391, 463, 646, 771, 979, 1438, 1729, 2002, 2056
DdeI CTNAG 7 cut(s) 58, 335, 1172, 1705, 2005, 2358, 2394
DinI GGCGCC 1 cut(s) 112
DraIII CACNNNGTG 1 cut(s) 2090
DrdI GACNNNNNNGTC 2 cut(s) 203, 2044
DriI GACNNNNNGTC 1 cut(s) 671
DseDI GACNNNNNNGTC 2 cut(s) 203, 2044
EaeI YGGCCR 2 cut(s) 744, 1459
Eam1104I CTCTTC 3 cut(s) 1328, 1375, 2156
Eam1105I GACNNNNNGTC 1 cut(s) 671
EarI CTCTTC 3 cut(s) 1328, 1375, 2156
EciI GGCGGA 2 cut(s) 911, 1345
Eco130I CCWWGG 3 cut(s) 747, 1456, 1920
Eco147I AGGCCT 2 cut(s) 1455, 2098
Eco24I GRGCYC 1 cut(s) 1308
Eco31I GGTCTC 2 cut(s) 211, 1330
Eco47I GGWCC 4 cut(s) 16, 665, 1109, 1742
Eco47III AGCGCT 1 cut(s) 2377
Eco57I CTGAAG 2 cut(s) 578, 2505
Eco81I CCTNAGG 1 cut(s) 2358
Eco88I CYCGRG 1 cut(s) 1510
Eco91I GGTNACC 1 cut(s) 959
EcoNI CCTNNNNNAGG 1 cut(s) 1251
EcoO109I RGGNCCY 2 cut(s) 665, 1293
EcoO65I GGTNACC 1 cut(s) 959
EcoRI GAATTC 1 cut(s) 1202
EcoRII CCWGG 2 cut(s) 12, 891
EcoT14I CCWWGG 3 cut(s) 747, 1456, 1920
EcoT22I ATGCAT 1 cut(s) 907
EcoT38I GRGCYC 1 cut(s) 1308
EgeI GGCGCC 1 cut(s) 112
EheI GGCGCC 1 cut(s) 112
ErhI CCWWGG 3 cut(s) 747, 1456, 1920
FaqI GGGAC 4 cut(s) 778, 1585, 1974, 2184
FauI CCCGC 3 cut(s) 339, 1235, 1820
FbaI TGATCA 2 cut(s) 570, 1906
FblI GTMKAC 1 cut(s) 213
FokI GGATG 6 cut(s) 315, 664, 1208, 2063, 2375, 2401
FriOI GRGCYC 1 cut(s) 1308
FspBI CTAG 6 cut(s) 252, 768, 1404, 1575, 1802, 2463
GlaI GCGC 3 cut(s) 112, 1132, 2377
HaeII RGCGCY 2 cut(s) 114, 2379
HaeIII GGCC 9 cut(s) 128, 298, 746, 1246, 1295, 1455, 1461, 2098, 2284
HapII CCGG 3 cut(s) 299, 599, 2111
HgaI GACGC 5 cut(s) 51, 391, 708, 728, 1958
HhaI GCGC 3 cut(s) 113, 1133, 2378
Hin1I GRCGYC 1 cut(s) 111
Hin6I GCGC 3 cut(s) 111, 1131, 2376
HinP1I GCGC 3 cut(s) 111, 1131, 2376
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HindIII AAGCTT 1 cut(s) 1993
HinfI GANTC 5 cut(s) 161, 167, 313, 1508, 2222
HpaII CCGG 3 cut(s) 299, 599, 2111
HphI GGTGA 3 cut(s) 565, 1174, 2504
Hpy166II GTNNAC 9 cut(s) 78, 214, 393, 418, 979, 1186, 1321, 2056, 2134
Hpy188I TCNGA 8 cut(s) 166, 1087, 1310, 1609, 1708, 2024, 2050, 2121
Hpy8I GTNNAC 9 cut(s) 78, 214, 393, 418, 979, 1186, 1321, 2056, 2134
Hpy99I CGWCG 4 cut(s) 760, 1880, 1957, 2495
HpyCH4III ACNGT 6 cut(s) 75, 1027, 1190, 1364, 1694, 2088
HpyCH4IV ACGT 4 cut(s) 225, 281, 755, 1878
HpyCH4V TGCA 7 cut(s) 827, 905, 1268, 2093, 2155, 2249, 2326
HpyF10VI GCNNNNNNNGC 9 cut(s) 304, 902, 1139, 1265, 1461, 1925, 2090, 2099, 2429
HpyF3I CTNAG 7 cut(s) 58, 335, 1172, 1705, 2005, 2358, 2394
HpySE526I ACGT 4 cut(s) 225, 281, 755, 1878
Hsp92I GRCGYC 1 cut(s) 111
HspAI GCGC 3 cut(s) 111, 1131, 2376
KasI GGCGCC 1 cut(s) 110
KroI GCCGGC 1 cut(s) 598
KroNI GCCGGC 1 cut(s) 600
Ksp22I TGATCA 2 cut(s) 570, 1906
LmnI GCTCC 3 cut(s) 562, 1310, 2125
LweI GCATC 3 cut(s) 259, 838, 1872
MaeI CTAG 6 cut(s) 252, 768, 1404, 1575, 1802, 2463
MaeII ACGT 4 cut(s) 225, 281, 755, 1878
MaeIII GTNAC 8 cut(s) 673, 935, 951, 959, 1017, 1482, 1677, 1694
MboII GAAGA 7 cut(s) 347, 1345, 1362, 2173, 2242, 2276, 2429
MfeI CAATTG 1 cut(s) 2033
MflI RGATCY 2 cut(s) 1638, 2207
MhlI GDGCHC 1 cut(s) 1308
MlsI TGGCCA 1 cut(s) 746
MluNI TGGCCA 1 cut(s) 746
Mly113I GGCGCC 1 cut(s) 111
MlyI GAGTC 1 cut(s) 1502
MmeI TCCRAC 2 cut(s) 2028, 2185
Mox20I TGGCCA 1 cut(s) 746
Mph1103I ATGCAT 1 cut(s) 907
MroNI GCCGGC 1 cut(s) 598
MroXI GAANNNNTTC 1 cut(s) 1364
MscI TGGCCA 1 cut(s) 746
MseI TTAA 8 cut(s) 654, 917, 984, 1029, 1283, 1658, 1881, 2214
MslI CAYNNNNRTG 5 cut(s) 545, 933, 1254, 1761, 1982
Msp20I TGGCCA 1 cut(s) 746
MspA1I CMGCKG 3 cut(s) 833, 1239, 2408
MspI CCGG 3 cut(s) 299, 599, 2111
MspR9I CCNGG 4 cut(s) 14, 300, 893, 2111
MunI CAATTG 1 cut(s) 2033
Mva1269I GAATGC 2 cut(s) 2157, 2339
MvaI CCWGG 2 cut(s) 14, 893
MvnI CGCG 4 cut(s) 1752, 1827, 1875, 1952
MwoI GCNNNNNNNGC 9 cut(s) 304, 902, 1139, 1265, 1461, 1925, 2090, 2099, 2429
NaeI GCCGGC 1 cut(s) 600
NarI GGCGCC 1 cut(s) 111
NciI CCSGG 2 cut(s) 300, 2111
NgoMIV GCCGGC 1 cut(s) 598
NheI GCTAGC 1 cut(s) 2462
NlaIV GGNNCC 4 cut(s) 112, 667, 1294, 2209
NmuCI GTSAC 6 cut(s) 673, 935, 951, 1017, 1677, 1694
NruI TCGCGA 1 cut(s) 1875
NsiI ATGCAT 1 cut(s) 907
NspI RCATGY 2 cut(s) 1438, 1735
OliI CACNNNNGTG 2 cut(s) 1254, 1982
PaeR7I CTCGAG 1 cut(s) 1510
PagI TCATGA 1 cut(s) 378
PceI AGGCCT 2 cut(s) 1455, 2098
PciI ACATGT 2 cut(s) 1434, 1731
PcsI WCGNNNNNNNCGW 1 cut(s) 1296
PctI GAATGC 2 cut(s) 2157, 2339
PdiI GCCGGC 1 cut(s) 600
PdmI GAANNNNTTC 1 cut(s) 1364
PfeI GAWTC 4 cut(s) 161, 167, 313, 2222
PflFI GACNNNGTC 1 cut(s) 1061
PleI GAGTC 1 cut(s) 1502
PluTI GGCGCC 1 cut(s) 114
PpsI GAGTC 1 cut(s) 1502
PpuMI RGGWCCY 1 cut(s) 665
PscI ACATGT 2 cut(s) 1434, 1731
Psp5II RGGWCCY 1 cut(s) 665
Psp6I CCWGG 2 cut(s) 12, 891
PspEI GGTNACC 1 cut(s) 959
PspGI CCWGG 2 cut(s) 12, 891
PspN4I GGNNCC 4 cut(s) 112, 667, 1294, 2209
PspPI GGNCC 7 cut(s) 16, 665, 1109, 1244, 1293, 1742, 2283
PspPPI RGGWCCY 1 cut(s) 665
PspXI VCTCGAGB 1 cut(s) 1510
PstI CTGCAG 2 cut(s) 1270, 2251
PstNI CAGNNNCTG 1 cut(s) 560
PsuI RGATCY 2 cut(s) 1638, 2207
PsyI GACNNNGTC 1 cut(s) 1061
RruI TCGCGA 1 cut(s) 1875
RsaI GTAC 9 cut(s) 392, 464, 647, 772, 980, 1439, 1730, 2003, 2057
RsaNI GTAC 9 cut(s) 391, 463, 646, 771, 979, 1438, 1729, 2002, 2056
RseI CAYNNNNRTG 5 cut(s) 545, 933, 1254, 1761, 1982
SalI GTCGAC 1 cut(s) 212
SaqAI TTAA 8 cut(s) 654, 917, 984, 1029, 1283, 1658, 1881, 2214
Sau96I GGNCC 7 cut(s) 16, 665, 1109, 1244, 1293, 1742, 2283
SchI GAGTC 1 cut(s) 1502
ScrFI CCNGG 4 cut(s) 14, 300, 893, 2111
SduI GDGCHC 1 cut(s) 1308
SfaNI GCATC 3 cut(s) 259, 838, 1872
SfcI CTRYAG 2 cut(s) 1266, 2247
SfoI GGCGCC 1 cut(s) 112
Sfr274I CTCGAG 1 cut(s) 1510
SinI GGWCC 4 cut(s) 16, 665, 1109, 1742
SlaI CTCGAG 1 cut(s) 1510
SmiMI CAYNNNNRTG 5 cut(s) 545, 933, 1254, 1761, 1982
SmlI CTYRAG 3 cut(s) 1341, 1510, 2346
SmoI CTYRAG 3 cut(s) 1341, 1510, 2346
SpeI ACTAGT 1 cut(s) 767
SseBI AGGCCT 2 cut(s) 1455, 2098
SspDI GGCGCC 1 cut(s) 110
SspI AATATT 1 cut(s) 2018
SspMI CTAG 6 cut(s) 252, 768, 1404, 1575, 1802, 2463
StuI AGGCCT 2 cut(s) 1455, 2098
StyD4I CCNGG 4 cut(s) 12, 298, 891, 2109
StyI CCWWGG 3 cut(s) 747, 1456, 1920
TaaI ACNGT 6 cut(s) 75, 1027, 1190, 1364, 1694, 2088
TaiI ACGT 4 cut(s) 228, 284, 758, 1881
TaqI TCGA 8 cut(s) 102, 213, 351, 922, 1415, 1511, 2106, 2531
TaqII GACCGA 1 cut(s) 1925
TatI WGTACW 6 cut(s) 390, 645, 978, 1437, 1728, 2055
TauI GCSGC 6 cut(s) 116, 527, 1242, 1397, 1464, 2408
TfiI GAWTC 4 cut(s) 161, 167, 313, 2222
Tru1I TTAA 8 cut(s) 654, 917, 984, 1029, 1283, 1658, 1881, 2214
Tru9I TTAA 8 cut(s) 654, 917, 984, 1029, 1283, 1658, 1881, 2214
TscAI CASTG 8 cut(s) 1030, 1686, 1852, 1921, 1984, 2091, 2162, 2398
TseFI GTSAC 6 cut(s) 673, 935, 951, 1017, 1677, 1694
Tsp45I GTSAC 6 cut(s) 673, 935, 951, 1017, 1677, 1694
TspGWI ACGGA 2 cut(s) 1171, 1770
TspRI CASTG 8 cut(s) 1030, 1686, 1852, 1921, 1984, 2091, 2162, 2398
Tth111I GACNNNGTC 1 cut(s) 1061
VpaK11BI GGWCC 4 cut(s) 16, 665, 1109, 1742
XagI CCTNNNNNAGG 1 cut(s) 1251
XapI RAATTY 5 cut(s) 447, 626, 868, 886, 1202
XceI RCATGY 2 cut(s) 1438, 1735
XhoI CTCGAG 1 cut(s) 1510
XmiI GTMKAC 1 cut(s) 213
XmnI GAANNNNTTC 1 cut(s) 1364
XspI CTAG 6 cut(s) 252, 768, 1404, 1575, 1802, 2463
Zsp2I ATGCAT 1 cut(s) 907
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.