FvH4_2g13190
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0013068
Physical Location & Seq
Reverse (-)
11536287 .. 11537405
1119 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g13190.t1

Sequence Viewer

Length: 1119 bp
ATGGACAAGACTTTCCAAGCCGTCCTCGCCGCCACCGGAAGCTTCTTCGCCATAACCGTAATCTTCGCCGTCGTCTACATACTCTTCAAGGGCTCCCAGTCCAAGTCAACCCGCTCCCGCCGCGCCCGTGCCGCCGCCGTCCGCAACAACCCGATCCGGCTCAACTGCATCACCGTCGACGAGATCGCCTCCTTCGATCCCTCCCTCAAAGTCTCCATGGAAGAAGTCGTCAAGGCCACCAAGAACTTCAACAAGGACCTCGTCGTCGGCGACGGCGGCTTCGGCCTCGTCTACAAGGCCCAGCTCTCCGACGGCCTCAAGGTCGCCATCAAGAAGCTCGATCCCGACGCCTTCCAGGGCTTCCGCGAGTTTCAGGCCGAGATGGAAACCCTAGGTGAGCTCCACCACCCCAATCTCGTCAAGATTCTCGGCTACTGTGTCTCCGGCGCCGACCGGATTTTGATTTATGAGTTTATGGAGAGGGGGAATCTGGACCGTTGGCTTCACGACTCGTCGTCAACGGATTTGGACGGTTATGATGACACGGCTGGGTGTAAATTACCGTTATCTTGGAAGACGAGGAACAAGGTCGTTAGGGGTGTGGCTAATGGCTTGGCCTATTTGCATGGGTTGGAGAAGCCTATAATTCATAGGGACATCAAGGCTAGCAATGTGTTGTTAGACAGGGAATATGAGGCCCATATTGCGGATTTCGGGCTCGCGAGGAGCATCGACACGTCACGGTCCCACGTGTCGACGCAATTTGCGGGCACCATGGGGTACATGCCACCCGAGTACAAAGACGGGTTTACGGGGGCGACAGTGAAAGCGGATGTGTTTAGTTTCGGGATTTTGATGTTGGAGATTGCAACGGGGAGACGGCCTAATTTGCCGAGCGTGTTTGACGGGAAAGAGATGGGGTTGGTGGAATGGTCGAGGAAAATGGTGGCCAATAACCGGCAAATGGAAATGGTGGATTCGAGTATTTCGAGGGAGGATTTGGATGAAGGCAGTGTCAAGAGGTACTTTGAAATTGTTGGTGAATGTGCTCATGAGTCTTCAAGGCAGAGGCCCCCAATGAAAGATGTAATTCAGCTGTTAAATGACCTTCAATCGTGA

Protein Analysis

373

Amino Acids

41.45

Weight (kDa)

6.47

Isoelectric Point (pI)

34.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 86 - 364 1.6e-40 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 88 - 366 1.9e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013068)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42440
fragaria_vesca FvH4_2g13190
malus_domestica MD05G1031600.v1.1 MD10G1033200.v1.1
prunus_persica Prupe.8G038700_v2.0.a1
pyrus_communis pycom05g02290 pycom10g02120 pycom10g02140
rosa_chinensis RchiOBHm_Chr6g0274481
rosa_laevigata RLG00000013540
rosa_multiflora Rmu_sc0001289.1_g000019
rosa_roxburghii Rroxscaffold_7G00194200
rosa_rugosa Rorug06G0082100
rosa_samantha Rh6AG198400 Rh6BG201700 Rh6CG202500 Rh6DG192900
rosa_wichuraiana Rw6G017250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 263
AccB1I GGYRCC 2 cut(s) 446, 770
AccBSI CCGCTC 1 cut(s) 114
AccI GTMKAC 4 cut(s) 75, 177, 291, 755
AccII CGCG 3 cut(s) 123, 366, 722
AclWI GGATC 3 cut(s) 148, 191, 335
AcoI YGGCCR 1 cut(s) 948
AcvI CACGTG 1 cut(s) 751
AcyI GRCGYC 2 cut(s) 348, 447
AfaI GTAC 3 cut(s) 782, 797, 1025
AfiI CCNNNNNNNGG 3 cut(s) 706, 957, 964
AflIII ACRYGT 2 cut(s) 735, 750
AgsI TTSAA 5 cut(s) 88, 250, 1031, 1062, 1112
AhdI GACNNNNNGTC 1 cut(s) 514
AjiI CACGTC 1 cut(s) 738
AjnI CCWGG 1 cut(s) 354
AluBI AGCT 5 cut(s) 42, 304, 337, 400, 1096
AluI AGCT 5 cut(s) 42, 304, 337, 400, 1096
Alw21I GWGCWC 2 cut(s) 402, 1051
Alw26I GTCTC 3 cut(s) 217, 445, 871
AlwI GGATC 3 cut(s) 148, 191, 335
Ama87I CYCGRG 1 cut(s) 791
ArsI GACNNNNNNTTYG 2 cut(s) 263, 295
AspA2I CCTAGG 1 cut(s) 391
AspLEI GCGC 2 cut(s) 125, 449
AspS9I GGNCC 6 cut(s) 256, 298, 493, 697, 744, 1071
AsuHPI GGTGA 3 cut(s) 163, 407, 1052
AsuNHI GCTAGC 1 cut(s) 665
AvaI CYCGRG 1 cut(s) 791
AvaII GGWCC 3 cut(s) 256, 493, 744
AvrII CCTAGG 1 cut(s) 391
BaeGI GKGCMC 1 cut(s) 773
BalI TGGCCA 1 cut(s) 950
BanI GGYRCC 2 cut(s) 446, 770
BanII GRGCYC 3 cut(s) 95, 402, 720
BbrPI CACGTG 1 cut(s) 751
BbsI GAAGAC 2 cut(s) 581, 1050
Bbv12I GWGCWC 2 cut(s) 402, 1051
BccI CCATC 3 cut(s) 335, 376, 910
BceAI ACGGC 7 cut(s) 5, 53, 122, 289, 328, 561, 896
BcgI CGANNNNNNTGC 2 cut(s) 157, 191
BciT130I CCWGG 1 cut(s) 356
BcoDI GTCTC 3 cut(s) 217, 445, 871
BfaI CTAG 2 cut(s) 392, 666
BfoI RGCGCY 1 cut(s) 450
BisI GCNGC 5 cut(s) 30, 121, 132, 135, 277
BlnI CCTAGG 1 cut(s) 391
BlsI GCNGC 5 cut(s) 31, 122, 133, 136, 278
Bme1390I CCNGG 1 cut(s) 356
Bme18I GGWCC 3 cut(s) 256, 493, 744
BmeRI GACNNNNNGTC 1 cut(s) 514
BmeT110I CYCGRG 1 cut(s) 791
BmgBI CACGTC 1 cut(s) 738
BmgT120I GGNCC 6 cut(s) 256, 298, 493, 697, 744, 1071
BmiI GGNNCC 5 cut(s) 94, 448, 746, 772, 1073
BmrFI CCNGG 1 cut(s) 356
BmrI ACTGGG 1 cut(s) 91
BmsI GCATC 2 cut(s) 177, 738
BmtI GCTAGC 1 cut(s) 669
BmuI ACTGGG 1 cut(s) 91
BpiI GAAGAC 2 cut(s) 581, 1050
BplI GAGNNNNNCTC 2 cut(s) 173, 205
BpuEI CTTGAG 1 cut(s) 302
BsaAI YACGTR 1 cut(s) 751
BsaHI GRCGYC 2 cut(s) 348, 447
BsaJI CCNNGG 4 cut(s) 216, 355, 391, 774
BsaWI WCCGGW 2 cut(s) 35, 453
BsaXI ACNNNNNCTCC 2 cut(s) 425, 455
Bsc4I CCNNNNNNNGG 3 cut(s) 706, 957, 964
Bse118I RCCGGY 1 cut(s) 957
Bse1I ACTGG 1 cut(s) 97
Bse3DI GCAATG 1 cut(s) 676
BseBI CCWGG 1 cut(s) 356
BseDI CCNNGG 4 cut(s) 216, 355, 391, 774
BseGI GGATG 2 cut(s) 838, 1009
BseLI CCNNNNNNNGG 3 cut(s) 706, 957, 964
BseMI GCAATG 1 cut(s) 676
BseNI ACTGG 1 cut(s) 97
BseRI GAGGAG 1 cut(s) 739
BseSI GKGCMC 1 cut(s) 773
BseYI CCCAGC 2 cut(s) 300, 548
Bsh1236I CGCG 3 cut(s) 123, 366, 722
Bsh1285I CGRYCG 1 cut(s) 454
BshNI GGYRCC 2 cut(s) 446, 770
BsiEI CGRYCG 1 cut(s) 454
BsiHKAI GWGCWC 2 cut(s) 402, 1051
BsiHKCI CYCGRG 1 cut(s) 791
BsiSI CCGG 5 cut(s) 36, 157, 444, 454, 958
BslFI GGGAC 2 cut(s) 668, 730
BslI CCNNNNNNNGG 3 cut(s) 706, 957, 964
BsmAI GTCTC 3 cut(s) 217, 445, 871
BsmBI CGTCTC 1 cut(s) 871
BsmFI GGGAC 2 cut(s) 668, 730
BsoBI CYCGRG 1 cut(s) 791
Bsp1286I GDGCHC 5 cut(s) 95, 402, 720, 773, 1051
Bsp143I GATC 4 cut(s) 153, 183, 196, 340
Bsp19I CCATGG 2 cut(s) 216, 774
Bsp68I TCGCGA 1 cut(s) 722
BspFNI CGCG 3 cut(s) 123, 366, 722
BspHI TCATGA 1 cut(s) 1051
BspLI GGNNCC 5 cut(s) 94, 448, 746, 772, 1073
BspOI GCTAGC 1 cut(s) 669
BspPI GGATC 3 cut(s) 148, 191, 335
BspT107I GGYRCC 2 cut(s) 446, 770
BsrBI CCGCTC 1 cut(s) 114
BsrDI GCAATG 1 cut(s) 676
BsrFI RCCGGY 1 cut(s) 957
BsrI ACTGG 1 cut(s) 97
BssAI RCCGGY 1 cut(s) 957
BssECI CCNNGG 4 cut(s) 216, 355, 391, 774
BssMI GATC 4 cut(s) 153, 183, 196, 340
BssNI GRCGYC 2 cut(s) 348, 447
BssT1I CCWWGG 3 cut(s) 216, 391, 774
Bst2UI CCWGG 1 cut(s) 356
Bst4CI ACNGT 8 cut(s) 58, 175, 437, 497, 533, 564, 744, 823
Bst6I CTCTTC 1 cut(s) 89
BstACI GRCGYC 2 cut(s) 348, 447
BstBAI YACGTR 1 cut(s) 751
BstC8I GCNNGC 3 cut(s) 667, 720, 769
BstDSI CCRYGG 2 cut(s) 216, 774
BstF5I GGATG 2 cut(s) 838, 1009
BstFNI CGCG 3 cut(s) 123, 366, 722
BstH2I RGCGCY 1 cut(s) 450
BstHHI GCGC 2 cut(s) 125, 449
BstKTI GATC 4 cut(s) 156, 186, 199, 343
BstMAI GTCTC 3 cut(s) 217, 445, 871
BstMBI GATC 4 cut(s) 153, 183, 196, 340
BstMCI CGRYCG 1 cut(s) 454
BstMWI GCNNNNNNNGC 7 cut(s) 26, 120, 131, 276, 282, 704, 889
BstNI CCWGG 1 cut(s) 356
BstNSI RCATGY 1 cut(s) 787
BstSCI CCNGG 1 cut(s) 354
BstSLI GKGCMC 1 cut(s) 773
BstUI CGCG 3 cut(s) 123, 366, 722
BstV2I GAAGAC 2 cut(s) 581, 1050
BtgI CCRYGG 2 cut(s) 216, 774
BtrI CACGTC 1 cut(s) 738
BtsCI GGATG 2 cut(s) 838, 1009
BtsI GCAGTG 1 cut(s) 1018
BtsIMutI CAGTG 2 cut(s) 828, 1018
BtuMI TCGCGA 1 cut(s) 722
Cac8I GCNNGC 3 cut(s) 667, 720, 769
CciI TCATGA 1 cut(s) 1051
CfoI GCGC 2 cut(s) 125, 449
Cfr10I RCCGGY 1 cut(s) 957
Cfr13I GGNCC 6 cut(s) 256, 298, 493, 697, 744, 1071
CseI GACGC 2 cut(s) 356, 766
Csp6I GTAC 3 cut(s) 781, 796, 1024
CviAII CATG 5 cut(s) 217, 626, 775, 784, 1052
CviQI GTAC 3 cut(s) 781, 796, 1024
DinI GGCGCC 1 cut(s) 448
DpnI GATC 4 cut(s) 155, 185, 198, 342
DpnII GATC 4 cut(s) 153, 183, 196, 340
DrdI GACNNNNNNGTC 1 cut(s) 263
DriI GACNNNNNGTC 1 cut(s) 514
DseDI GACNNNNNNGTC 1 cut(s) 263
EaeI YGGCCR 1 cut(s) 948
Eam1104I CTCTTC 1 cut(s) 89
Eam1105I GACNNNNNGTC 1 cut(s) 514
EarI CTCTTC 1 cut(s) 89
Ecl136II GAGCTC 1 cut(s) 400
Eco130I CCWWGG 3 cut(s) 216, 391, 774
Eco24I GRGCYC 3 cut(s) 95, 402, 720
Eco47I GGWCC 3 cut(s) 256, 493, 744
Eco53kI GAGCTC 1 cut(s) 400
Eco72I CACGTG 1 cut(s) 751
Eco88I CYCGRG 1 cut(s) 791
EcoICRI GAGCTC 1 cut(s) 400
EcoO109I RGGNCCY 2 cut(s) 256, 1071
EcoRII CCWGG 1 cut(s) 354
EcoT14I CCWWGG 3 cut(s) 216, 391, 774
EcoT38I GRGCYC 3 cut(s) 95, 402, 720
EgeI GGCGCC 1 cut(s) 448
EheI GGCGCC 1 cut(s) 448
ErhI CCWWGG 3 cut(s) 216, 391, 774
Esp3I CGTCTC 1 cut(s) 871
FaeI CATG 5 cut(s) 220, 629, 778, 787, 1055
FalI AAGNNNNNCTT 2 cut(s) 1010, 1042
FaqI GGGAC 2 cut(s) 668, 730
FatI CATG 5 cut(s) 216, 625, 774, 783, 1051
FauI CCCGC 3 cut(s) 119, 125, 760
FblI GTMKAC 4 cut(s) 75, 177, 291, 755
Fnu4HI GCNGC 5 cut(s) 30, 121, 132, 135, 277
FokI GGATG 2 cut(s) 845, 1016
FriOI GRGCYC 3 cut(s) 95, 402, 720
Fsp4HI GCNGC 5 cut(s) 30, 121, 132, 135, 277
FspBI CTAG 2 cut(s) 392, 666
GlaI GCGC 2 cut(s) 124, 448
GluI GCNGC 5 cut(s) 30, 121, 132, 135, 277
GsaI CCCAGC 2 cut(s) 304, 552
HaeII RGCGCY 1 cut(s) 450
HapII CCGG 5 cut(s) 36, 157, 444, 454, 958
HgaI GACGC 2 cut(s) 356, 766
HhaI GCGC 2 cut(s) 125, 449
Hin1I GRCGYC 2 cut(s) 348, 447
Hin1II CATG 5 cut(s) 220, 629, 778, 787, 1055
Hin6I GCGC 2 cut(s) 123, 447
HinP1I GCGC 2 cut(s) 123, 447
HincII GTYRAC 4 cut(s) 108, 178, 519, 756
HindII GTYRAC 4 cut(s) 108, 178, 519, 756
HindIII AAGCTT 1 cut(s) 40
HinfI GANTC 5 cut(s) 424, 487, 509, 977, 1055
HpaII CCGG 5 cut(s) 36, 157, 444, 454, 958
HphI GGTGA 3 cut(s) 163, 407, 1052
Hpy166II GTNNAC 7 cut(s) 76, 108, 178, 292, 519, 756, 810
Hpy188I TCNGA 1 cut(s) 310
Hpy8I GTNNAC 7 cut(s) 76, 108, 178, 292, 519, 756, 810
HpyAV CCTTC 4 cut(s) 202, 361, 1001, 1118
HpyCH4III ACNGT 8 cut(s) 58, 175, 437, 497, 533, 564, 744, 823
HpyCH4IV ACGT 2 cut(s) 737, 750
HpyCH4V TGCA 3 cut(s) 168, 625, 869
HpyF10VI GCNNNNNNNGC 7 cut(s) 26, 120, 131, 276, 282, 704, 889
HpySE526I ACGT 2 cut(s) 737, 750
Hsp92I GRCGYC 2 cut(s) 348, 447
Hsp92II CATG 5 cut(s) 220, 629, 778, 787, 1055
HspAI GCGC 2 cut(s) 123, 447
KasI GGCGCC 1 cut(s) 446
Kzo9I GATC 4 cut(s) 153, 183, 196, 340
LmnI GCTCC 4 cut(s) 98, 119, 405, 726
LweI GCATC 2 cut(s) 177, 738
MaeI CTAG 2 cut(s) 392, 666
MaeII ACGT 2 cut(s) 737, 750
MaeIII GTNAC 1 cut(s) 738
MalI GATC 4 cut(s) 155, 185, 198, 342
MbiI CCGCTC 1 cut(s) 114
MboI GATC 4 cut(s) 153, 183, 196, 340
MboII GAAGA 6 cut(s) 37, 55, 76, 233, 586, 1050
MhlI GDGCHC 5 cut(s) 95, 402, 720, 773, 1051
MlsI TGGCCA 1 cut(s) 950
MluCI AATT 6 cut(s) 557, 645, 761, 886, 1032, 1089
MluNI TGGCCA 1 cut(s) 950
Mly113I GGCGCC 1 cut(s) 447
MlyI GAGTC 2 cut(s) 503, 1064
MmeI TCCRAC 3 cut(s) 333, 612, 840
Mox20I TGGCCA 1 cut(s) 950
MscI TGGCCA 1 cut(s) 950
MseI TTAA 1 cut(s) 1100
Msp20I TGGCCA 1 cut(s) 950
MspA1I CMGCKG 1 cut(s) 1096
MspI CCGG 5 cut(s) 36, 157, 444, 454, 958
MspR9I CCNGG 1 cut(s) 356
MvaI CCWGG 1 cut(s) 356
MvnI CGCG 3 cut(s) 123, 366, 722
MwoI GCNNNNNNNGC 7 cut(s) 26, 120, 131, 276, 282, 704, 889
NarI GGCGCC 1 cut(s) 447
NcoI CCATGG 2 cut(s) 216, 774
NdeII GATC 4 cut(s) 153, 183, 196, 340
NheI GCTAGC 1 cut(s) 665
NlaIII CATG 5 cut(s) 220, 629, 778, 787, 1055
NlaIV GGNNCC 5 cut(s) 94, 448, 746, 772, 1073
NmeAIII GCCGAG 3 cut(s) 403, 408, 918
NmuCI GTSAC 1 cut(s) 738
NruI TCGCGA 1 cut(s) 722
NspI RCATGY 1 cut(s) 787
PagI TCATGA 1 cut(s) 1051
PcsI WCGNNNNNNNCGW 4 cut(s) 54, 192, 267, 986
PfeI GAWTC 3 cut(s) 424, 487, 977
PflFI GACNNNGTC 1 cut(s) 260
PkrI GCNGC 5 cut(s) 31, 122, 133, 136, 278
PleI GAGTC 2 cut(s) 503, 1063
PluTI GGCGCC 1 cut(s) 450
PmaCI CACGTG 1 cut(s) 751
PmlI CACGTG 1 cut(s) 751
PpsI GAGTC 2 cut(s) 503, 1063
Ppu21I YACGTR 1 cut(s) 751
PpuMI RGGWCCY 1 cut(s) 256
Psp124BI GAGCTC 1 cut(s) 402
Psp5II RGGWCCY 1 cut(s) 256
Psp6I CCWGG 1 cut(s) 354
PspCI CACGTG 1 cut(s) 751
PspFI CCCAGC 2 cut(s) 300, 548
PspGI CCWGG 1 cut(s) 354
PspN4I GGNNCC 5 cut(s) 94, 448, 746, 772, 1073
PspPI GGNCC 6 cut(s) 256, 298, 493, 697, 744, 1071
PspPPI RGGWCCY 1 cut(s) 256
PsyI GACNNNGTC 1 cut(s) 260
PvuII CAGCTG 1 cut(s) 1096
RruI TCGCGA 1 cut(s) 722
RsaI GTAC 3 cut(s) 782, 797, 1025
RsaNI GTAC 3 cut(s) 781, 796, 1024
SacI GAGCTC 1 cut(s) 402
SalI GTCGAC 2 cut(s) 176, 754
SaqAI TTAA 1 cut(s) 1100
SatI GCNGC 5 cut(s) 30, 121, 132, 135, 277
Sau3AI GATC 4 cut(s) 153, 183, 196, 340
Sau96I GGNCC 6 cut(s) 256, 298, 493, 697, 744, 1071
SchI GAGTC 2 cut(s) 503, 1064
ScrFI CCNGG 1 cut(s) 356
SduI GDGCHC 5 cut(s) 95, 402, 720, 773, 1051
SfaNI GCATC 2 cut(s) 177, 738
SfoI GGCGCC 1 cut(s) 448
SgrDI CGTCGACG 1 cut(s) 176
SinI GGWCC 3 cut(s) 256, 493, 744
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
Sse9I AATT 6 cut(s) 557, 645, 761, 886, 1032, 1089
SspDI GGCGCC 1 cut(s) 446
SspMI CTAG 2 cut(s) 392, 666
SstI GAGCTC 1 cut(s) 402
StyD4I CCNGG 1 cut(s) 354
StyI CCWWGG 3 cut(s) 216, 391, 774
TaaI ACNGT 8 cut(s) 58, 175, 437, 497, 533, 564, 744, 823
TaiI ACGT 2 cut(s) 740, 753
TaqI TCGA 8 cut(s) 177, 195, 339, 732, 755, 935, 980, 989
TasI AATT 6 cut(s) 557, 645, 761, 886, 1032, 1089
TatI WGTACW 1 cut(s) 795
TauI GCSGC 5 cut(s) 32, 123, 134, 137, 279
TfiI GAWTC 3 cut(s) 424, 487, 977
Tru1I TTAA 1 cut(s) 1100
Tru9I TTAA 1 cut(s) 1100
TscAI CASTG 2 cut(s) 828, 1018
TseFI GTSAC 1 cut(s) 738
Tsp45I GTSAC 1 cut(s) 738
TspDTI ATGAA 3 cut(s) 638, 1020, 1094
TspGWI ACGGA 1 cut(s) 536
TspRI CASTG 2 cut(s) 828, 1018
Tth111I GACNNNGTC 1 cut(s) 260
VpaK11BI GGWCC 3 cut(s) 256, 493, 744
XceI RCATGY 1 cut(s) 787
XmaJI CCTAGG 1 cut(s) 391
XmiI GTMKAC 4 cut(s) 75, 177, 291, 755
XspI CTAG 2 cut(s) 392, 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.