Rroxscaffold_7G00194200
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0013068
Physical Location & Seq
Forward (+)
35964904 .. 35966022
1119 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00194200.1

Sequence Viewer

Length: 1119 bp
ATGGAAAAGACTCTCCAAGCCGTTCTCGCCGCCACCGGAAGCTTCTTCGCCATAACCGTAATCTTCGCCTCCGTCTACATACTCTGCAAGGGCGCCAAGTCCAAGTCCACCGGCTCCCGGAACACGAACCGGGCAGTCCGCAACCGGACGACCCAGCTCACCTACATCACCGTGGACGAGAGCGCCTCCTTCGATCCCTCTCTCAAGGTCTCCATGGATGAAGTGGTCAAGGCCACCAAGAACTTCAACAAGGACCTCGTAGTCGGCGACGGCGGCTTCGGCCTCGTCTACAAGGCCCAGCTCTCCGACGGCCTCAAGGTCGCCATCAAGAAGCTCGATCCCGACGCCTTTCAAGGCTTCCGCGAGTTTCGGGCCGAGATGGAAACCCTAGGTCAGCTCTGGCACCCCAATCTTGTTAGGATTCTCGGATATTGCGTGTCCGGCGCCGATAGGATTCTGATTTACGAGTTTATGGAGAGGGGGAAATCTGGACCGTTGGCTTCACACGACTCCTCGTCAACCGACTTTGATGGCTATGATGACACGGCTGGGTGTAAATTGCCGTTATCTTGGAAAACGAGGAATAAGATCGTTAGGGGCGTGGCCAATGGGTTGGGCTATTTGCACAGCTTGGAGAAGCCCATAATCCATAGGGATATCAAGGCTAGCAATGTTTTGTTGGACAGGGAGTACGAAGCCCATATTGCGGATTTCGGGCTCGCGAGGAGCATCGACATGTCCCGTTCCCACGTGTCGACGCAATTCGCGGGTACCATGGGGTACATGCCGCCCGAGTACAAAGAAGGTAACACGGTGGCGACCGTGAAGGCAGATGTGTATAGTTTTGGGATTTTGATGTTGGAGGTTGCGACCGGAAGACGGCCAAATTTGCCGAGCGTGTTTGACGGAAAAGAGATGGGATTGGTGGAATGGGCGAGGAAAATGGTGGCCCAAAACCGGCAAATGGAAATGGTGGATCCGAGTATTTCAAGGGAGGATTTGGATGAAGGACATGTCAAGAAGTATTTTGAAATTGCTGGTGAATGTGCTCATGAGTCTTCAAGGCAAAGGCCCCCAATGAAAGATGTCATTGGGCTGTTAAATGAGCTTCCGACGTGA

Protein Analysis

372

Amino Acids

41.23

Weight (kDa)

7.09

Isoelectric Point (pI)

27.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 85 - 297 1.1e-38 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 87 - 364 1.2e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013068)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42440
fragaria_vesca FvH4_2g13190
malus_domestica MD05G1031600.v1.1 MD10G1033200.v1.1
prunus_persica Prupe.8G038700_v2.0.a1
pyrus_communis pycom05g02290 pycom10g02120 pycom10g02140
rosa_chinensis RchiOBHm_Chr6g0274481
rosa_laevigata RLG00000013540
rosa_multiflora Rmu_sc0001289.1_g000019
rosa_roxburghii Rroxscaffold_7G00194200
rosa_rugosa Rorug06G0082100
rosa_samantha Rh6AG198400 Rh6BG201700 Rh6CG202500 Rh6DG192900
rosa_wichuraiana Rw6G017250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 260
Acc65I GGTACC 1 cut(s) 770
AccB1I GGYRCC 4 cut(s) 92, 402, 443, 770
AccI GTMKAC 3 cut(s) 75, 288, 755
AccII CGCG 3 cut(s) 363, 722, 767
AciI CCGC 7 cut(s) 30, 139, 273, 361, 707, 767, 788
AclWI GGATC 4 cut(s) 188, 332, 971, 984
AcoI YGGCCR 2 cut(s) 603, 881
AcsI RAATTY 1 cut(s) 886
AcvI CACGTG 1 cut(s) 751
AcyI GRCGYC 3 cut(s) 93, 345, 444
AfaI GTAC 4 cut(s) 692, 772, 782, 797
AfiI CCNNNNNNNGG 5 cut(s) 117, 706, 879, 957, 964
AflIII ACRYGT 3 cut(s) 735, 750, 1012
AgsI TTSAA 5 cut(s) 247, 353, 990, 1031, 1062
AhdI GACNNNNNGTC 1 cut(s) 514
AjiI CACGTC 1 cut(s) 1116
AluBI AGCT 7 cut(s) 42, 157, 301, 334, 397, 630, 1108
AluI AGCT 7 cut(s) 42, 157, 301, 334, 397, 630, 1108
Alw21I GWGCWC 1 cut(s) 1051
Alw26I GTCTC 1 cut(s) 214
AlwI GGATC 4 cut(s) 188, 332, 971, 984
Ama87I CYCGRG 1 cut(s) 791
AoxI GGCC 9 cut(s) 231, 280, 294, 310, 372, 603, 881, 948, 1070
ApoI RAATTY 1 cut(s) 886
ArsI GACNNNNNNTTYG 2 cut(s) 260, 292
Asp718I GGTACC 1 cut(s) 770
AspA2I CCTAGG 1 cut(s) 388
AspLEI GCGC 3 cut(s) 95, 185, 446
AspS9I GGNCC 6 cut(s) 253, 295, 372, 491, 949, 1071
AsuC2I CCSGG 2 cut(s) 118, 131
AsuHPI GGTGA 3 cut(s) 151, 160, 1052
AsuNHI GCTAGC 1 cut(s) 665
AvaI CYCGRG 1 cut(s) 791
AvaII GGWCC 2 cut(s) 253, 491
AvrII CCTAGG 1 cut(s) 388
BalI TGGCCA 1 cut(s) 605
BamHI GGATCC 1 cut(s) 976
BanI GGYRCC 4 cut(s) 92, 402, 443, 770
BanII GRGCYC 1 cut(s) 720
BbrPI CACGTG 1 cut(s) 751
BbsI GAAGAC 2 cut(s) 883, 1050
Bbv12I GWGCWC 1 cut(s) 1051
BccI CCATC 4 cut(s) 332, 373, 524, 910
BceAI ACGGC 6 cut(s) 5, 286, 325, 547, 561, 896
BcnI CCSGG 2 cut(s) 118, 131
BcoDI GTCTC 1 cut(s) 214
BfaI CTAG 2 cut(s) 389, 666
BfoI RGCGCY 3 cut(s) 96, 186, 447
BisI GCNGC 3 cut(s) 30, 274, 788
BlnI CCTAGG 1 cut(s) 388
BlsI GCNGC 3 cut(s) 31, 275, 789
Bme1390I CCNGG 2 cut(s) 118, 131
Bme18I GGWCC 2 cut(s) 253, 491
BmeRI GACNNNNNGTC 1 cut(s) 514
BmeT110I CYCGRG 1 cut(s) 791
BmgBI CACGTC 1 cut(s) 1116
BmgT120I GGNCC 6 cut(s) 253, 295, 372, 491, 949, 1071
BmiI GGNNCC 7 cut(s) 94, 115, 404, 445, 772, 978, 1073
BmrFI CCNGG 2 cut(s) 118, 131
BmsI GCATC 1 cut(s) 738
BmtI GCTAGC 1 cut(s) 669
BpiI GAAGAC 2 cut(s) 883, 1050
BplI GAGNNNNNCTC 2 cut(s) 170, 202
BpuEI CTTGAG 2 cut(s) 188, 299
BpuMI CCSGG 2 cut(s) 118, 131
BsaAI YACGTR 1 cut(s) 751
BsaHI GRCGYC 3 cut(s) 93, 345, 444
BsaI GGTCTC 1 cut(s) 214
BsaJI CCNNGG 4 cut(s) 171, 213, 388, 774
BsaWI WCCGGW 3 cut(s) 35, 144, 872
Bsc4I CCNNNNNNNGG 5 cut(s) 117, 706, 879, 957, 964
Bse118I RCCGGY 2 cut(s) 110, 957
Bse3DI GCAATG 1 cut(s) 676
BseDI CCNNGG 4 cut(s) 171, 213, 388, 774
BseGI GGATG 2 cut(s) 223, 1009
BseLI CCNNNNNNNGG 5 cut(s) 117, 706, 879, 957, 964
BseMI GCAATG 1 cut(s) 676
BseRI GAGGAG 2 cut(s) 502, 739
BseYI CCCAGC 3 cut(s) 153, 297, 548
Bsh1236I CGCG 3 cut(s) 363, 722, 767
Bsh1285I CGRYCG 2 cut(s) 822, 873
BshFI GGCC 9 cut(s) 233, 282, 296, 312, 374, 605, 883, 950, 1072
BshNI GGYRCC 4 cut(s) 92, 402, 443, 770
BsiEI CGRYCG 2 cut(s) 822, 873
BsiHKAI GWGCWC 1 cut(s) 1051
BsiHKCI CYCGRG 1 cut(s) 791
BsiSI CCGG 8 cut(s) 36, 111, 118, 130, 145, 441, 873, 958
BslFI GGGAC 1 cut(s) 724
BslI CCNNNNNNNGG 5 cut(s) 117, 706, 879, 957, 964
BsmAI GTCTC 1 cut(s) 214
BsmFI GGGAC 1 cut(s) 724
BsnI GGCC 9 cut(s) 233, 282, 296, 312, 374, 605, 883, 950, 1072
Bso31I GGTCTC 1 cut(s) 214
BsoBI CYCGRG 1 cut(s) 791
Bsp1286I GDGCHC 2 cut(s) 720, 1051
Bsp143I GATC 4 cut(s) 193, 337, 588, 976
Bsp19I CCATGG 2 cut(s) 213, 774
Bsp68I TCGCGA 1 cut(s) 722
BspACI CCGC 7 cut(s) 30, 139, 273, 361, 707, 767, 788
BspANI GGCC 9 cut(s) 233, 282, 296, 312, 374, 605, 883, 950, 1072
BspFNI CGCG 3 cut(s) 363, 722, 767
BspHI TCATGA 1 cut(s) 1051
BspLI GGNNCC 7 cut(s) 94, 115, 404, 445, 772, 978, 1073
BspOI GCTAGC 1 cut(s) 669
BspPI GGATC 4 cut(s) 188, 332, 971, 984
BspT107I GGYRCC 4 cut(s) 92, 402, 443, 770
BspTNI GGTCTC 1 cut(s) 214
BsrDI GCAATG 1 cut(s) 676
BsrFI RCCGGY 2 cut(s) 110, 957
BssAI RCCGGY 2 cut(s) 110, 957
BssECI CCNNGG 4 cut(s) 171, 213, 388, 774
BssMI GATC 4 cut(s) 193, 337, 588, 976
BssNI GRCGYC 3 cut(s) 93, 345, 444
BssT1I CCWWGG 3 cut(s) 213, 388, 774
Bst4CI ACNGT 5 cut(s) 58, 172, 495, 814, 823
BstACI GRCGYC 3 cut(s) 93, 345, 444
BstBAI YACGTR 1 cut(s) 751
BstC8I GCNNGC 2 cut(s) 667, 720
BstDSI CCRYGG 3 cut(s) 171, 213, 774
BstF5I GGATG 2 cut(s) 223, 1009
BstFNI CGCG 3 cut(s) 363, 722, 767
BstH2I RGCGCY 3 cut(s) 96, 186, 447
BstHHI GCGC 3 cut(s) 95, 185, 446
BstKTI GATC 4 cut(s) 196, 340, 591, 979
BstMAI GTCTC 1 cut(s) 214
BstMBI GATC 4 cut(s) 193, 337, 588, 976
BstMCI CGRYCG 2 cut(s) 822, 873
BstMWI GCNNNNNNNGC 6 cut(s) 26, 273, 279, 441, 704, 889
BstNSI RCATGY 3 cut(s) 739, 787, 1016
BstSCI CCNGG 2 cut(s) 116, 129
BstUI CGCG 3 cut(s) 363, 722, 767
BstV2I GAAGAC 2 cut(s) 883, 1050
BstX2I RGATCY 1 cut(s) 976
BstXI CCANNNNNNTGG 1 cut(s) 613
BstYI RGATCY 1 cut(s) 976
BsuRI GGCC 9 cut(s) 233, 282, 296, 312, 374, 605, 883, 950, 1072
BtgI CCRYGG 3 cut(s) 171, 213, 774
BtrI CACGTC 1 cut(s) 1116
BtsCI GGATG 2 cut(s) 223, 1009
BtuMI TCGCGA 1 cut(s) 722
Cac8I GCNNGC 2 cut(s) 667, 720
CciI TCATGA 1 cut(s) 1051
CfoI GCGC 3 cut(s) 95, 185, 446
Cfr10I RCCGGY 2 cut(s) 110, 957
Cfr13I GGNCC 6 cut(s) 253, 295, 372, 491, 949, 1071
CseI GACGC 2 cut(s) 353, 766
Csp6I GTAC 4 cut(s) 691, 771, 781, 796
CviAII CATG 6 cut(s) 214, 736, 775, 784, 1013, 1052
CviQI GTAC 4 cut(s) 691, 771, 781, 796
DinI GGCGCC 2 cut(s) 94, 445
DpnI GATC 4 cut(s) 195, 339, 590, 978
DpnII GATC 4 cut(s) 193, 337, 588, 976
DrdI GACNNNNNNGTC 1 cut(s) 260
DriI GACNNNNNGTC 1 cut(s) 514
DseDI GACNNNNNNGTC 1 cut(s) 260
EaeI YGGCCR 2 cut(s) 603, 881
Eam1105I GACNNNNNGTC 1 cut(s) 514
Eco130I CCWWGG 3 cut(s) 213, 388, 774
Eco24I GRGCYC 1 cut(s) 720
Eco31I GGTCTC 1 cut(s) 214
Eco32I GATATC 1 cut(s) 658
Eco47I GGWCC 2 cut(s) 253, 491
Eco72I CACGTG 1 cut(s) 751
Eco88I CYCGRG 1 cut(s) 791
EcoO109I RGGNCCY 2 cut(s) 253, 1071
EcoRV GATATC 1 cut(s) 658
EcoT14I CCWWGG 3 cut(s) 213, 388, 774
EcoT38I GRGCYC 1 cut(s) 720
EgeI GGCGCC 2 cut(s) 94, 445
EheI GGCGCC 2 cut(s) 94, 445
ErhI CCWWGG 3 cut(s) 213, 388, 774
FaeI CATG 6 cut(s) 217, 739, 778, 787, 1016, 1055
FaqI GGGAC 1 cut(s) 724
FatI CATG 6 cut(s) 213, 735, 774, 783, 1012, 1051
FauI CCCGC 1 cut(s) 760
FblI GTMKAC 3 cut(s) 75, 288, 755
Fnu4HI GCNGC 3 cut(s) 30, 274, 788
FokI GGATG 2 cut(s) 230, 1016
FriOI GRGCYC 1 cut(s) 720
Fsp4HI GCNGC 3 cut(s) 30, 274, 788
FspBI CTAG 2 cut(s) 389, 666
GlaI GCGC 3 cut(s) 94, 184, 445
GluI GCNGC 3 cut(s) 30, 274, 788
GsaI CCCAGC 3 cut(s) 157, 301, 552
HaeII RGCGCY 3 cut(s) 96, 186, 447
HaeIII GGCC 9 cut(s) 233, 282, 296, 312, 374, 605, 883, 950, 1072
HapII CCGG 8 cut(s) 36, 111, 118, 130, 145, 441, 873, 958
HgaI GACGC 2 cut(s) 353, 766
HhaI GCGC 3 cut(s) 95, 185, 446
Hin1I GRCGYC 3 cut(s) 93, 345, 444
Hin1II CATG 6 cut(s) 217, 739, 778, 787, 1016, 1055
Hin6I GCGC 3 cut(s) 93, 183, 444
HinP1I GCGC 3 cut(s) 93, 183, 444
HincII GTYRAC 2 cut(s) 519, 756
HindII GTYRAC 2 cut(s) 519, 756
HindIII AAGCTT 1 cut(s) 40
HinfI GANTC 5 cut(s) 10, 421, 454, 509, 1055
HpaII CCGG 8 cut(s) 36, 111, 118, 130, 145, 441, 873, 958
HphI GGTGA 3 cut(s) 151, 160, 1052
Hpy166II GTNNAC 6 cut(s) 76, 108, 175, 289, 519, 756
Hpy188I TCNGA 5 cut(s) 307, 428, 459, 981, 1113
Hpy188III TCNNGA 6 cut(s) 328, 341, 489, 721, 1018, 1052
Hpy8I GTNNAC 6 cut(s) 76, 108, 175, 289, 519, 756
Hpy99I CGWCG 5 cut(s) 272, 311, 347, 760, 1117
HpyAV CCTTC 4 cut(s) 199, 797, 820, 1001
HpyCH4III ACNGT 5 cut(s) 58, 172, 495, 814, 823
HpyCH4IV ACGT 2 cut(s) 750, 1115
HpyCH4V TGCA 2 cut(s) 87, 625
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 273, 279, 441, 704, 889
HpySE526I ACGT 2 cut(s) 750, 1115
Hsp92I GRCGYC 3 cut(s) 93, 345, 444
Hsp92II CATG 6 cut(s) 217, 739, 778, 787, 1016, 1055
HspAI GCGC 3 cut(s) 93, 183, 444
KasI GGCGCC 2 cut(s) 92, 443
KpnI GGTACC 1 cut(s) 774
Kzo9I GATC 4 cut(s) 193, 337, 588, 976
LmnI GCTCC 2 cut(s) 119, 726
LweI GCATC 1 cut(s) 738
MaeI CTAG 2 cut(s) 389, 666
MaeII ACGT 2 cut(s) 750, 1115
MaeIII GTNAC 1 cut(s) 806
MalI GATC 4 cut(s) 195, 339, 590, 978
MboI GATC 4 cut(s) 193, 337, 588, 976
MboII GAAGA 4 cut(s) 37, 55, 888, 1050
MflI RGATCY 1 cut(s) 976
MhlI GDGCHC 2 cut(s) 720, 1051
MlsI TGGCCA 1 cut(s) 605
MluCI AATT 4 cut(s) 557, 761, 886, 1032
MluNI TGGCCA 1 cut(s) 605
Mly113I GGCGCC 2 cut(s) 93, 444
MlyI GAGTC 3 cut(s) 4, 503, 1064
MmeI TCCRAC 3 cut(s) 330, 660, 840
Mox20I TGGCCA 1 cut(s) 605
MscI TGGCCA 1 cut(s) 605
MseI TTAA 1 cut(s) 1100
MslI CAYNNNNRTG 2 cut(s) 170, 734
Msp20I TGGCCA 1 cut(s) 605
MspI CCGG 8 cut(s) 36, 111, 118, 130, 145, 441, 873, 958
MspR9I CCNGG 2 cut(s) 118, 131
MvnI CGCG 3 cut(s) 363, 722, 767
MwoI GCNNNNNNNGC 6 cut(s) 26, 273, 279, 441, 704, 889
NarI GGCGCC 2 cut(s) 93, 444
NciI CCSGG 2 cut(s) 118, 131
NcoI CCATGG 2 cut(s) 213, 774
NdeII GATC 4 cut(s) 193, 337, 588, 976
NheI GCTAGC 1 cut(s) 665
NlaIII CATG 6 cut(s) 217, 739, 778, 787, 1016, 1055
NlaIV GGNNCC 7 cut(s) 94, 115, 404, 445, 772, 978, 1073
NmeAIII GCCGAG 2 cut(s) 400, 918
NruI TCGCGA 1 cut(s) 722
NspI RCATGY 3 cut(s) 739, 787, 1016
PagI TCATGA 1 cut(s) 1051
PciI ACATGT 2 cut(s) 735, 1012
PcsI WCGNNNNNNNCGW 4 cut(s) 54, 264, 432, 597
PfeI GAWTC 2 cut(s) 421, 454
PfoI TCCNGGA 1 cut(s) 116
PkrI GCNGC 3 cut(s) 31, 275, 789
PleI GAGTC 3 cut(s) 4, 503, 1063
PluTI GGCGCC 2 cut(s) 96, 447
PmaCI CACGTG 1 cut(s) 751
PmlI CACGTG 1 cut(s) 751
PpsI GAGTC 3 cut(s) 4, 503, 1063
Ppu21I YACGTR 1 cut(s) 751
PpuMI RGGWCCY 1 cut(s) 253
PscI ACATGT 2 cut(s) 735, 1012
Psp5II RGGWCCY 1 cut(s) 253
PspCI CACGTG 1 cut(s) 751
PspFI CCCAGC 3 cut(s) 153, 297, 548
PspN4I GGNNCC 7 cut(s) 94, 115, 404, 445, 772, 978, 1073
PspPI GGNCC 6 cut(s) 253, 295, 372, 491, 949, 1071
PspPPI RGGWCCY 1 cut(s) 253
PsuI RGATCY 1 cut(s) 976
RruI TCGCGA 1 cut(s) 722
RsaI GTAC 4 cut(s) 692, 772, 782, 797
RsaNI GTAC 4 cut(s) 691, 771, 781, 796
RseI CAYNNNNRTG 2 cut(s) 170, 734
SalI GTCGAC 1 cut(s) 754
SaqAI TTAA 1 cut(s) 1100
SatI GCNGC 3 cut(s) 30, 274, 788
Sau3AI GATC 4 cut(s) 193, 337, 588, 976
Sau96I GGNCC 6 cut(s) 253, 295, 372, 491, 949, 1071
SchI GAGTC 3 cut(s) 4, 503, 1064
ScrFI CCNGG 2 cut(s) 118, 131
SduI GDGCHC 2 cut(s) 720, 1051
SfaNI GCATC 1 cut(s) 738
SfoI GGCGCC 2 cut(s) 94, 445
SinI GGWCC 2 cut(s) 253, 491
SmiMI CAYNNNNRTG 2 cut(s) 170, 734
SmlI CTYRAG 2 cut(s) 203, 314
SmoI CTYRAG 2 cut(s) 203, 314
Sse9I AATT 4 cut(s) 557, 761, 886, 1032
SsiI CCGC 7 cut(s) 30, 139, 273, 361, 707, 767, 788
SspDI GGCGCC 2 cut(s) 92, 443
SspMI CTAG 2 cut(s) 389, 666
StyD4I CCNGG 2 cut(s) 116, 129
StyI CCWWGG 3 cut(s) 213, 388, 774
TaaI ACNGT 5 cut(s) 58, 172, 495, 814, 823
TaiI ACGT 2 cut(s) 753, 1118
TaqI TCGA 4 cut(s) 192, 336, 732, 755
TasI AATT 4 cut(s) 557, 761, 886, 1032
TatI WGTACW 1 cut(s) 795
TauI GCSGC 3 cut(s) 32, 276, 790
TfiI GAWTC 2 cut(s) 421, 454
Tru1I TTAA 1 cut(s) 1100
Tru9I TTAA 1 cut(s) 1100
TspDTI ATGAA 3 cut(s) 234, 1020, 1094
TspGWI ACGGA 2 cut(s) 61, 921
VpaK11BI GGWCC 2 cut(s) 253, 491
XapI RAATTY 1 cut(s) 886
XceI RCATGY 3 cut(s) 739, 787, 1016
XcmI CCANNNNNNNNNTGG 1 cut(s) 220
XmaJI CCTAGG 1 cut(s) 388
XmiI GTMKAC 3 cut(s) 75, 288, 755
XspI CTAG 2 cut(s) 389, 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.