MD10G1033200.v1.1
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0013068
Physical Location & Seq
Forward (+)
4268840 .. 4269955
1116 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1033200.v1.1.491

Sequence Viewer

Length: 1116 bp
ATGTATATGGACAAACCTGTCCAATCCGTCCTCGCTGCTACCGGCGGCTTCTTCGTCGTAACCCTCATCCTCGCCGCCGTCTACCTCGTCTGCAAGGACTGCAAATCCAAATCCACCCGCTCCCGCCCCGCCCGTCCCCTCTGCAACCCGGCCGCCCTCTCCGCCATCATCGTCGACGAGAGTACCTCCTTCGATCCGTGCCTCAAGTTCTCCATGGAAGAAGTCGTCAAGGCCACCAAGAACTTCTCCGCCCATCTCGTTGTCGGTGACGGCGGCTTCGGCCTTGTCTACAAGGCCCAACTCCCCGACGGCCTCACCGTCGCGATCAAGAAGCTGGATCCCGACGCCTTCCAAGGGTTTCGGGAATTTCAGGCCGAGATGGAAACCCTAGGTAAGCTCCGCCACCCCAACATCGTCAAGATTTTAGGATACTGCGTCTCCGGCGCCGACAGGATATTAATTTATGAGTTTATTTCAAAGGGAAATCTCGACCGTTGGCTTTATGACTCGTGGTCGTCGGACGGCGACGGTTATGAGGACGGGCCGGGGTTGAGATTACCGTTATCTTGGAAAACGAGGATTAAGATCGTTAGGGGAGTGGCGAATGGGTTGGCATATTTGCATGGGCTTGAAAAGCCCATTATTCATAGGGATATTAAGGCCAGCAATGTGTTGTTGGACAAAGATTTTGAGGCCCATATTGCTGATTTTGGGCTGGCCAGAAGTATGGACGCCTCCCGGTCTCACGTGTCGACTCAATTTGCCGGCACGATGGGTTACATGCCCCCGGAATACAAGGAAGGGTTCACGGGGGCAACCGTCAGGGCGGACGTGTTTAGCTTTGGGATCTTAATGTTGGAGGTTGCGACCGGGAGGCGGCCAAATTTGCCGACGGTGCACGACGGGAACCAAATGGGGTTGGTGGAATGGGCTAGGAAAATGGTGGAACAAAATCGGCAAATGGAAATGGTGGATTCAAGCATTTCGAATGAGGATTTGGATGAAGGGGAAGTGAAGGAGTATTTTAGCATTGCTACTACATGTGCTCGTGAGATTTCAAGAGACAGGCCCGCCATGAAAGAAGTCATTGAAATGTTGGACCAAATTTCGACATGA

Protein Analysis

372

Amino Acids

41.13

Weight (kDa)

5.74

Isoelectric Point (pI)

32.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 87 - 366 4.4e-45 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 87 - 364 6.5e-40 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013068)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42440
fragaria_vesca FvH4_2g13190
malus_domestica MD05G1031600.v1.1 MD10G1033200.v1.1
prunus_persica Prupe.8G038700_v2.0.a1
pyrus_communis pycom05g02290 pycom10g02120 pycom10g02140
rosa_chinensis RchiOBHm_Chr6g0274481
rosa_laevigata RLG00000013540
rosa_multiflora Rmu_sc0001289.1_g000019
rosa_roxburghii Rroxscaffold_7G00194200
rosa_rugosa Rorug06G0082100
rosa_samantha Rh6AG198400 Rh6BG201700 Rh6CG202500 Rh6DG192900
rosa_wichuraiana Rw6G017250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 17
AccB1I GGYRCC 1 cut(s) 443
AccBSI CCGCTC 1 cut(s) 120
AccI GTMKAC 4 cut(s) 81, 174, 288, 752
AccII CGCG 1 cut(s) 323
AclWI GGATC 4 cut(s) 188, 332, 345, 854
AcoI YGGCCR 3 cut(s) 150, 717, 878
AcsI RAATTY 3 cut(s) 365, 883, 1104
AcvI CACGTG 1 cut(s) 748
AcyI GRCGYC 3 cut(s) 345, 444, 732
AfaI GTAC 1 cut(s) 184
AfiI CCNNNNNNNGG 2 cut(s) 354, 876
AflIII ACRYGT 3 cut(s) 747, 831, 1040
AgsI TTSAA 5 cut(s) 477, 632, 978, 1059, 1091
AhdI GACNNNNNGTC 1 cut(s) 511
AjiI CACGTC 1 cut(s) 832
AjuI GAANNNNNNNTTGG 2 cut(s) 980, 1012
AluBI AGCT 3 cut(s) 334, 397, 840
AluI AGCT 3 cut(s) 334, 397, 840
Alw21I GWGCWC 2 cut(s) 900, 1048
Alw26I GTCTC 3 cut(s) 442, 747, 1056
Alw44I GTGCAC 1 cut(s) 896
AlwI GGATC 4 cut(s) 188, 332, 345, 854
ApaLI GTGCAC 1 cut(s) 896
ApeKI GCWGC 1 cut(s) 35
ApoI RAATTY 3 cut(s) 365, 883, 1104
ArsI GACNNNNNNTTYG 4 cut(s) 260, 292, 671, 703
AseI ATTAAT 1 cut(s) 458
AspA2I CCTAGG 1 cut(s) 388
AspLEI GCGC 1 cut(s) 446
AspS9I GGNCC 5 cut(s) 295, 542, 694, 1068, 1099
AsuC2I CCSGG 5 cut(s) 149, 546, 739, 788, 871
AsuHPI GGTGA 2 cut(s) 278, 307
AsuII TTCGAA 1 cut(s) 986
AvaII GGWCC 1 cut(s) 1099
AvrII CCTAGG 1 cut(s) 388
BaeGI GKGCMC 1 cut(s) 900
BaeI ACNNNNGTAYC 2 cut(s) 166, 199
BalI TGGCCA 1 cut(s) 719
BamHI GGATCC 1 cut(s) 337
BanI GGYRCC 1 cut(s) 443
BauI CACGAG 2 cut(s) 508, 1047
BbrPI CACGTG 1 cut(s) 748
Bbv12I GWGCWC 2 cut(s) 900, 1048
BbvI GCAGC 1 cut(s) 22
BccI CCATC 4 cut(s) 173, 261, 373, 766
BceAI ACGGC 4 cut(s) 62, 286, 325, 538
BciVI GTATCC 1 cut(s) 422
BcnI CCSGG 5 cut(s) 149, 546, 739, 788, 871
BcoDI GTCTC 3 cut(s) 442, 747, 1056
BfaI CTAG 2 cut(s) 389, 933
BfoI RGCGCY 1 cut(s) 447
BfuI GTATCC 1 cut(s) 422
BisI GCNGC 6 cut(s) 36, 46, 75, 153, 274, 878
BlnI CCTAGG 1 cut(s) 388
BlsI GCNGC 6 cut(s) 37, 47, 76, 154, 275, 879
Bme1390I CCNGG 5 cut(s) 149, 546, 739, 788, 871
Bme18I GGWCC 1 cut(s) 1099
BmeRI GACNNNNNGTC 1 cut(s) 511
BmgBI CACGTC 1 cut(s) 832
BmgT120I GGNCC 5 cut(s) 295, 542, 694, 1068, 1099
BmiI GGNNCC 3 cut(s) 339, 445, 908
BmrFI CCNGG 5 cut(s) 149, 546, 739, 788, 871
BplI GAGNNNNNCTC 2 cut(s) 170, 202
Bpu14I TTCGAA 1 cut(s) 986
BpuEI CTTGAG 1 cut(s) 188
BpuMI CCSGG 5 cut(s) 149, 546, 739, 788, 871
BsaAI YACGTR 1 cut(s) 748
BsaBI GATNNNNATC 1 cut(s) 584
BsaHI GRCGYC 3 cut(s) 345, 444, 732
BsaI GGTCTC 1 cut(s) 747
BsaJI CCNNGG 5 cut(s) 213, 352, 388, 545, 786
BsaXI ACNNNNNCTCC 2 cut(s) 422, 452
Bsc4I CCNNNNNNNGG 2 cut(s) 354, 876
Bse118I RCCGGY 2 cut(s) 41, 764
Bse3DI GCAATG 2 cut(s) 673, 1029
Bse8I GATNNNNATC 1 cut(s) 584
BseDI CCNNGG 5 cut(s) 213, 352, 388, 545, 786
BseGI GGATG 2 cut(s) 66, 1006
BseJI GATNNNNATC 1 cut(s) 584
BseLI CCNNNNNNNGG 2 cut(s) 354, 876
BseMI GCAATG 2 cut(s) 673, 1029
BseSI GKGCMC 1 cut(s) 900
BseX3I CGGCCG 1 cut(s) 150
BseXI GCAGC 1 cut(s) 22
Bsh1236I CGCG 1 cut(s) 323
Bsh1285I CGRYCG 3 cut(s) 153, 493, 870
BshNI GGYRCC 1 cut(s) 443
BsiEI CGRYCG 3 cut(s) 153, 493, 870
BsiHKAI GWGCWC 2 cut(s) 900, 1048
BsiSI CCGG 8 cut(s) 42, 149, 441, 545, 739, 765, 788, 870
BslFI GGGAC 1 cut(s) 120
BslI CCNNNNNNNGG 2 cut(s) 354, 876
BsmAI GTCTC 3 cut(s) 442, 747, 1056
BsmBI CGTCTC 1 cut(s) 442
BsmFI GGGAC 1 cut(s) 120
Bso31I GGTCTC 1 cut(s) 747
Bsp119I TTCGAA 1 cut(s) 986
Bsp1286I GDGCHC 2 cut(s) 900, 1048
Bsp143I GATC 5 cut(s) 193, 324, 337, 585, 846
Bsp19I CCATGG 1 cut(s) 213
Bsp68I TCGCGA 1 cut(s) 323
BspFNI CGCG 1 cut(s) 323
BspLI GGNNCC 3 cut(s) 339, 445, 908
BspPI GGATC 4 cut(s) 188, 332, 345, 854
BspT104I TTCGAA 1 cut(s) 986
BspT107I GGYRCC 1 cut(s) 443
BspTNI GGTCTC 1 cut(s) 747
BsrBI CCGCTC 1 cut(s) 120
BsrDI GCAATG 2 cut(s) 673, 1029
BsrFI RCCGGY 2 cut(s) 41, 764
BssAI RCCGGY 2 cut(s) 41, 764
BssECI CCNNGG 5 cut(s) 213, 352, 388, 545, 786
BssMI GATC 5 cut(s) 193, 324, 337, 585, 846
BssNI GRCGYC 3 cut(s) 345, 444, 732
BssSI CACGAG 2 cut(s) 508, 1047
BssT1I CCWWGG 3 cut(s) 213, 352, 388
Bst2BI CACGAG 2 cut(s) 508, 1047
Bst4CI ACNGT 6 cut(s) 319, 494, 530, 561, 820, 895
BstACI GRCGYC 3 cut(s) 345, 444, 732
BstAPI GCANNNNNTGC 1 cut(s) 99
BstBAI YACGTR 1 cut(s) 748
BstBI TTCGAA 1 cut(s) 986
BstC8I GCNNGC 4 cut(s) 664, 717, 766, 1071
BstDSI CCRYGG 1 cut(s) 213
BstF5I GGATG 2 cut(s) 66, 1006
BstFNI CGCG 1 cut(s) 323
BstH2I RGCGCY 1 cut(s) 447
BstHHI GCGC 1 cut(s) 446
BstKTI GATC 5 cut(s) 196, 327, 340, 588, 849
BstMAI GTCTC 3 cut(s) 442, 747, 1056
BstMBI GATC 5 cut(s) 193, 324, 337, 585, 846
BstMCI CGRYCG 3 cut(s) 153, 493, 870
BstMWI GCNNNNNNNGC 8 cut(s) 99, 161, 279, 441, 634, 701, 886, 895
BstNSI RCATGY 2 cut(s) 784, 1044
BstSCI CCNGG 5 cut(s) 147, 544, 737, 786, 869
BstSLI GKGCMC 1 cut(s) 900
BstUI CGCG 1 cut(s) 323
BstV1I GCAGC 1 cut(s) 22
BstX2I RGATCY 2 cut(s) 337, 846
BstXI CCANNNNNNTGG 1 cut(s) 727
BstYI RGATCY 2 cut(s) 337, 846
BstZI CGGCCG 1 cut(s) 150
BsuI GTATCC 1 cut(s) 422
BtgI CCRYGG 1 cut(s) 213
BtrI CACGTC 1 cut(s) 832
BtsCI GGATG 2 cut(s) 66, 1006
BtuMI TCGCGA 1 cut(s) 323
Cac8I GCNNGC 4 cut(s) 664, 717, 766, 1071
CfoI GCGC 1 cut(s) 446
Cfr10I RCCGGY 2 cut(s) 41, 764
Cfr13I GGNCC 5 cut(s) 295, 542, 694, 1068, 1099
CseI GACGC 3 cut(s) 353, 424, 740
Csp6I GTAC 1 cut(s) 183
CviAII CATG 6 cut(s) 214, 623, 781, 1041, 1075, 1113
CviQI GTAC 1 cut(s) 183
DinI GGCGCC 1 cut(s) 445
DpnI GATC 5 cut(s) 195, 326, 339, 587, 848
DpnII GATC 5 cut(s) 193, 324, 337, 585, 846
DrdI GACNNNNNNGTC 1 cut(s) 17
DriI GACNNNNNGTC 1 cut(s) 511
DseDI GACNNNNNNGTC 1 cut(s) 17
EaeI YGGCCR 3 cut(s) 150, 717, 878
EagI CGGCCG 1 cut(s) 150
Eam1105I GACNNNNNGTC 1 cut(s) 511
EciI GGCGGA 4 cut(s) 151, 238, 389, 842
EclXI CGGCCG 1 cut(s) 150
Eco130I CCWWGG 3 cut(s) 213, 352, 388
Eco31I GGTCTC 1 cut(s) 747
Eco47I GGWCC 1 cut(s) 1099
Eco52I CGGCCG 1 cut(s) 150
Eco72I CACGTG 1 cut(s) 748
EcoT14I CCWWGG 3 cut(s) 213, 352, 388
EgeI GGCGCC 1 cut(s) 445
EheI GGCGCC 1 cut(s) 445
ErhI CCWWGG 3 cut(s) 213, 352, 388
Esp3I CGTCTC 1 cut(s) 442
FaeI CATG 6 cut(s) 217, 626, 784, 1044, 1078, 1116
FaqI GGGAC 1 cut(s) 120
FatI CATG 6 cut(s) 213, 622, 780, 1040, 1074, 1112
FauI CCCGC 4 cut(s) 125, 131, 136, 1078
FblI GTMKAC 4 cut(s) 81, 174, 288, 752
Fnu4HI GCNGC 6 cut(s) 36, 46, 75, 153, 274, 878
FokI GGATG 2 cut(s) 53, 1013
Fsp4HI GCNGC 6 cut(s) 36, 46, 75, 153, 274, 878
FspBI CTAG 2 cut(s) 389, 933
GlaI GCGC 1 cut(s) 445
GluI GCNGC 6 cut(s) 36, 46, 75, 153, 274, 878
HaeII RGCGCY 1 cut(s) 447
HapII CCGG 8 cut(s) 42, 149, 441, 545, 739, 765, 788, 870
HgaI GACGC 3 cut(s) 353, 424, 740
HhaI GCGC 1 cut(s) 446
Hin1I GRCGYC 3 cut(s) 345, 444, 732
Hin1II CATG 6 cut(s) 217, 626, 784, 1044, 1078, 1116
Hin6I GCGC 1 cut(s) 444
HinP1I GCGC 1 cut(s) 444
HincII GTYRAC 2 cut(s) 175, 753
HindII GTYRAC 2 cut(s) 175, 753
HinfI GANTC 3 cut(s) 506, 754, 974
HpaII CCGG 8 cut(s) 42, 149, 441, 545, 739, 765, 788, 870
HphI GGTGA 2 cut(s) 278, 307
Hpy166II GTNNAC 6 cut(s) 82, 175, 289, 753, 807, 898
Hpy188I TCNGA 1 cut(s) 520
Hpy188III TCNNGA 8 cut(s) 322, 328, 341, 362, 418, 488, 1049, 1059
Hpy8I GTNNAC 6 cut(s) 82, 175, 289, 753, 807, 898
HpyAV CCTTC 5 cut(s) 199, 358, 794, 998, 1009
HpyCH4III ACNGT 6 cut(s) 319, 494, 530, 561, 820, 895
HpyCH4IV ACGT 2 cut(s) 747, 831
HpyCH4V TGCA 5 cut(s) 93, 102, 144, 622, 898
HpyF10VI GCNNNNNNNGC 8 cut(s) 99, 161, 279, 441, 634, 701, 886, 895
HpySE526I ACGT 2 cut(s) 747, 831
Hsp92I GRCGYC 3 cut(s) 345, 444, 732
Hsp92II CATG 6 cut(s) 217, 626, 784, 1044, 1078, 1116
HspAI GCGC 1 cut(s) 444
KasI GGCGCC 1 cut(s) 443
KroI GCCGGC 1 cut(s) 764
KroNI GCCGGC 1 cut(s) 766
Kzo9I GATC 5 cut(s) 193, 324, 337, 585, 846
LmnI GCTCC 2 cut(s) 125, 402
Lsp1109I GCAGC 1 cut(s) 22
MaeI CTAG 2 cut(s) 389, 933
MaeII ACGT 2 cut(s) 747, 831
MaeIII GTNAC 3 cut(s) 58, 266, 776
MalI GATC 5 cut(s) 195, 326, 339, 587, 848
MbiI CCGCTC 1 cut(s) 120
MboI GATC 5 cut(s) 193, 324, 337, 585, 846
MboII GAAGA 2 cut(s) 43, 230
MflI RGATCY 2 cut(s) 337, 846
MhlI GDGCHC 2 cut(s) 900, 1048
MlsI TGGCCA 1 cut(s) 719
MluCI AATT 5 cut(s) 365, 459, 758, 883, 1104
MluNI TGGCCA 1 cut(s) 719
Mly113I GGCGCC 1 cut(s) 444
MlyI GAGTC 2 cut(s) 500, 748
MmeI TCCRAC 4 cut(s) 498, 657, 837, 1077
Mox20I TGGCCA 1 cut(s) 719
MroNI GCCGGC 1 cut(s) 764
MscI TGGCCA 1 cut(s) 719
MseI TTAA 4 cut(s) 458, 582, 657, 851
MslI CAYNNNNRTG 1 cut(s) 1091
Msp20I TGGCCA 1 cut(s) 719
MspI CCGG 8 cut(s) 42, 149, 441, 545, 739, 765, 788, 870
MspR9I CCNGG 5 cut(s) 149, 546, 739, 788, 871
MvnI CGCG 1 cut(s) 323
MwoI GCNNNNNNNGC 8 cut(s) 99, 161, 279, 441, 634, 701, 886, 895
NaeI GCCGGC 1 cut(s) 766
NarI GGCGCC 1 cut(s) 444
NciI CCSGG 5 cut(s) 149, 546, 739, 788, 871
NcoI CCATGG 1 cut(s) 213
NdeII GATC 5 cut(s) 193, 324, 337, 585, 846
NgoMIV GCCGGC 1 cut(s) 764
NlaIII CATG 6 cut(s) 217, 626, 784, 1044, 1078, 1116
NlaIV GGNNCC 3 cut(s) 339, 445, 908
NmeAIII GCCGAG 1 cut(s) 400
NmuCI GTSAC 1 cut(s) 266
NruI TCGCGA 1 cut(s) 323
NspI RCATGY 2 cut(s) 784, 1044
NspV TTCGAA 1 cut(s) 986
PciI ACATGT 1 cut(s) 1040
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PdiI GCCGGC 1 cut(s) 766
PfeI GAWTC 1 cut(s) 974
PkrI GCNGC 6 cut(s) 37, 47, 76, 154, 275, 879
PleI GAGTC 2 cut(s) 500, 748
PluTI GGCGCC 1 cut(s) 447
PmaCI CACGTG 1 cut(s) 748
PmlI CACGTG 1 cut(s) 748
PpsI GAGTC 2 cut(s) 500, 748
Ppu21I YACGTR 1 cut(s) 748
PscI ACATGT 1 cut(s) 1040
PshBI ATTAAT 1 cut(s) 458
PspCI CACGTG 1 cut(s) 748
PspN4I GGNNCC 3 cut(s) 339, 445, 908
PspPI GGNCC 5 cut(s) 295, 542, 694, 1068, 1099
PsuI RGATCY 2 cut(s) 337, 846
RruI TCGCGA 1 cut(s) 323
RsaI GTAC 1 cut(s) 184
RsaNI GTAC 1 cut(s) 183
RseI CAYNNNNRTG 1 cut(s) 1091
SalI GTCGAC 2 cut(s) 173, 751
SaqAI TTAA 4 cut(s) 458, 582, 657, 851
SatI GCNGC 6 cut(s) 36, 46, 75, 153, 274, 878
Sau3AI GATC 5 cut(s) 193, 324, 337, 585, 846
Sau96I GGNCC 5 cut(s) 295, 542, 694, 1068, 1099
SchI GAGTC 2 cut(s) 500, 748
ScrFI CCNGG 5 cut(s) 149, 546, 739, 788, 871
SduI GDGCHC 2 cut(s) 900, 1048
SfoI GGCGCC 1 cut(s) 445
SfuI TTCGAA 1 cut(s) 986
SgrDI CGTCGACG 1 cut(s) 173
SinI GGWCC 1 cut(s) 1099
SmiMI CAYNNNNRTG 1 cut(s) 1091
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
Sse9I AATT 5 cut(s) 365, 459, 758, 883, 1104
SspDI GGCGCC 1 cut(s) 443
SspMI CTAG 2 cut(s) 389, 933
StyD4I CCNGG 5 cut(s) 147, 544, 737, 786, 869
StyI CCWWGG 3 cut(s) 213, 352, 388
TaaI ACNGT 6 cut(s) 319, 494, 530, 561, 820, 895
TaiI ACGT 2 cut(s) 750, 834
TaqI TCGA 6 cut(s) 174, 192, 489, 752, 986, 1109
TasI AATT 5 cut(s) 365, 459, 758, 883, 1104
TauI GCSGC 5 cut(s) 48, 77, 155, 276, 880
TfiI GAWTC 1 cut(s) 974
Tru1I TTAA 4 cut(s) 458, 582, 657, 851
Tru9I TTAA 4 cut(s) 458, 582, 657, 851
TseFI GTSAC 1 cut(s) 266
TseI GCWGC 1 cut(s) 35
Tsp45I GTSAC 1 cut(s) 266
TspDTI ATGAA 3 cut(s) 635, 1017, 1091
TspGWI ACGGA 2 cut(s) 16, 186
VneI GTGCAC 1 cut(s) 896
VpaK11BI GGWCC 1 cut(s) 1099
VspI ATTAAT 1 cut(s) 458
XapI RAATTY 3 cut(s) 365, 883, 1104
XceI RCATGY 2 cut(s) 784, 1044
XmaJI CCTAGG 1 cut(s) 388
XmiI GTMKAC 4 cut(s) 81, 174, 288, 752
XspI CTAG 2 cut(s) 389, 933
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.