FvH4_2g14000

CDP-diacylglycerol-inositol 3-phosphatidyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005659
Physical Location & Seq
Forward (+)
12307042 .. 12311526
4485 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g14000.t1

Sequence Viewer

Length: 690 bp
ATGGCCAAGAAATCAGTACCACAACCAAAGCTAACAAAGCTTTCTGTCTACCTTTACATACCTAATATAATTGGGTACATTAGGGTTCTTATGAACTGTTATGCTTTTGCCATATGCTTCTCCAACAAATCACTTTTCTCCATTCTCTATTTTGCCAGCTTTGTCTGTGATGGAGTAGATGGTTGGTGTGCTCGCAAATTCAATCAAGTTTCAACATTTGGAGCCGTTTTGGACATGGTAACAGACAGGATAAGCACTGCATGTCTCTTGGTAATTCTGTCACAAGTCTATCGGCCTGGATTGATTTTCCTGTCGTTGCTTGCCTTAGATATTGGTAGCCACTGGCTGCAGATGTACAGTACATTCCTACTTGGCAAGGCTAGTCACAAAGATGTGAAAGACAGCACTAATTGGCTTTTCAGAGCATACTACGGGAATCGGATGTTTATGGCTTACTGTTGTGTGGCATGTGAGGTGCTTTATATCATTCTATTTCTTGTTGCAAGGAACAAATCTGAGAATTTGATTGATGTTTTAATGGGTCTTGCACACCATATTACACCTCTTTCTGTTCTAGTTGGTTTGAGTTTATTTGGATGGGCTGTCAAACAAGCAGTCAATTGTATACAGTTGAAGACAGCAGCAGATGTGTGTGTGCTTCATGATATCAACAAGAAGCACAGCTCATAA

Protein Analysis

230

Amino Acids

25.71

Weight (kDa)

9.07

Isoelectric Point (pI)

31.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDP-OH_P_transf PF01066 19 - 114 2e-15 CDP-alcohol phosphatidyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 48, 625
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 197, 520
AfaI GTAC 4 cut(s) 18, 77, 356, 361
AgsI TTSAA 3 cut(s) 202, 213, 634
AjnI CCWGG 1 cut(s) 295
AluBI AGCT 4 cut(s) 31, 40, 159, 684
AluI AGCT 4 cut(s) 31, 40, 159, 684
Alw21I GWGCWC 1 cut(s) 193
Alw26I GTCTC 1 cut(s) 269
AoxI GGCC 2 cut(s) 3, 293
ApeKI GCWGC 2 cut(s) 346, 641
ApoI RAATTY 2 cut(s) 197, 520
ArsI GACNNNNNNTTYG 2 cut(s) 600, 632
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 641
Bbv12I GWGCWC 1 cut(s) 193
BbvI GCAGC 2 cut(s) 333, 653
BccI CCATC 3 cut(s) 164, 173, 591
BceAI ACGGC 1 cut(s) 209
BciT130I CCWGG 1 cut(s) 297
BcoDI GTCTC 1 cut(s) 269
BfaI CTAG 2 cut(s) 381, 575
BfmI CTRYAG 1 cut(s) 347
BisI GCNGC 2 cut(s) 347, 642
BlsI GCNGC 2 cut(s) 348, 643
Bme1390I CCNGG 1 cut(s) 297
BmiI GGNNCC 1 cut(s) 223
BmrFI CCNGG 1 cut(s) 297
BpiI GAAGAC 1 cut(s) 641
Bse1I ACTGG 1 cut(s) 347
BseBI CCWGG 1 cut(s) 297
BseGI GGATG 2 cut(s) 447, 602
BseMII CTCAG 1 cut(s) 507
BseNI ACTGG 1 cut(s) 347
BseXI GCAGC 2 cut(s) 333, 653
BshFI GGCC 2 cut(s) 5, 295
BsiHKAI GWGCWC 1 cut(s) 193
BsmAI GTCTC 1 cut(s) 269
BsnI GGCC 2 cut(s) 5, 295
Bsp1286I GDGCHC 1 cut(s) 193
Bsp1407I TGTACA 1 cut(s) 354
BspANI GGCC 2 cut(s) 5, 295
BspCNI CTCAG 1 cut(s) 508
BspHI TCATGA 1 cut(s) 661
BspLI GGNNCC 1 cut(s) 223
BspMAI CTGCAG 1 cut(s) 351
BsrGI TGTACA 1 cut(s) 354
BsrI ACTGG 1 cut(s) 347
BssNAI GTATAC 1 cut(s) 626
Bst1107I GTATAC 1 cut(s) 626
Bst2UI CCWGG 1 cut(s) 297
Bst4CI ACNGT 4 cut(s) 98, 359, 458, 630
BstAUI TGTACA 1 cut(s) 354
BstC8I GCNNGC 3 cut(s) 157, 193, 321
BstDEI CTNAG 2 cut(s) 325, 516
BstF5I GGATG 2 cut(s) 447, 602
BstMAI GTCTC 1 cut(s) 269
BstMWI GCNNNNNNNGC 1 cut(s) 37
BstNI CCWGG 1 cut(s) 297
BstNSI RCATGY 2 cut(s) 264, 471
BstSCI CCNGG 1 cut(s) 295
BstSFI CTRYAG 1 cut(s) 347
BstV1I GCAGC 2 cut(s) 333, 653
BstV2I GAAGAC 1 cut(s) 641
BstZ17I GTATAC 1 cut(s) 626
BsuRI GGCC 2 cut(s) 5, 295
BtsCI GGATG 2 cut(s) 447, 602
BtsI GCAGTG 1 cut(s) 255
BtsIMutI CAGTG 2 cut(s) 255, 340
Cac8I GCNNGC 3 cut(s) 157, 193, 321
CciI TCATGA 1 cut(s) 661
Csp6I GTAC 4 cut(s) 17, 76, 355, 360
CviAII CATG 4 cut(s) 235, 261, 468, 662
CviQI GTAC 4 cut(s) 17, 76, 355, 360
DdeI CTNAG 2 cut(s) 325, 516
EaeI YGGCCR 1 cut(s) 3
Eco32I GATATC 1 cut(s) 667
EcoRII CCWGG 1 cut(s) 295
EcoRV GATATC 1 cut(s) 667
FaeI CATG 4 cut(s) 238, 264, 471, 665
FatI CATG 4 cut(s) 234, 260, 467, 661
FauNDI CATATG 1 cut(s) 113
FblI GTMKAC 2 cut(s) 48, 625
Fnu4HI GCNGC 2 cut(s) 347, 642
FokI GGATG 2 cut(s) 454, 609
Fsp4HI GCNGC 2 cut(s) 347, 642
FspBI CTAG 2 cut(s) 381, 575
GluI GCNGC 2 cut(s) 347, 642
HaeIII GGCC 2 cut(s) 5, 295
Hin1II CATG 4 cut(s) 238, 264, 471, 665
HindIII AAGCTT 1 cut(s) 38
HinfI GANTC 1 cut(s) 436
Hpy166II GTNNAC 2 cut(s) 49, 626
Hpy188I TCNGA 3 cut(s) 422, 441, 517
Hpy188III TCNNGA 1 cut(s) 662
Hpy8I GTNNAC 2 cut(s) 49, 626
HpyCH4III ACNGT 4 cut(s) 98, 359, 458, 630
HpyCH4V TGCA 4 cut(s) 260, 349, 503, 548
HpyF10VI GCNNNNNNNGC 1 cut(s) 37
HpyF3I CTNAG 2 cut(s) 325, 516
Hsp92II CATG 4 cut(s) 238, 264, 471, 665
LmnI GCTCC 1 cut(s) 221
LpnPI CCDG 6 cut(s) 169, 232, 282, 309, 323, 328
Lsp1109I GCAGC 2 cut(s) 333, 653
MaeI CTAG 2 cut(s) 381, 575
MaeIII GTNAC 3 cut(s) 238, 279, 383
MboII GAAGA 1 cut(s) 646
MfeI CAATTG 1 cut(s) 619
MhlI GDGCHC 1 cut(s) 193
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 69, 197, 273, 409, 520, 619
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 147
MnlI CCTC 2 cut(s) 466, 573
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 536
MslI CAYNNNNRTG 1 cut(s) 390
Msp20I TGGCCA 1 cut(s) 5
MspR9I CCNGG 1 cut(s) 297
MunI CAATTG 1 cut(s) 619
MvaI CCWGG 1 cut(s) 297
MwoI GCNNNNNNNGC 1 cut(s) 37
NdeI CATATG 1 cut(s) 113
NlaIII CATG 4 cut(s) 238, 264, 471, 665
NlaIV GGNNCC 1 cut(s) 223
NmuCI GTSAC 2 cut(s) 279, 383
NspI RCATGY 2 cut(s) 264, 471
PagI TCATGA 1 cut(s) 661
PfeI GAWTC 1 cut(s) 436
PkrI GCNGC 2 cut(s) 348, 643
Psp6I CCWGG 1 cut(s) 295
PspGI CCWGG 1 cut(s) 295
PspN4I GGNNCC 1 cut(s) 223
PstI CTGCAG 1 cut(s) 351
RsaI GTAC 4 cut(s) 18, 77, 356, 361
RsaNI GTAC 4 cut(s) 17, 76, 355, 360
RseI CAYNNNNRTG 1 cut(s) 390
SaqAI TTAA 1 cut(s) 536
SatI GCNGC 2 cut(s) 347, 642
ScrFI CCNGG 1 cut(s) 297
SduI GDGCHC 1 cut(s) 193
SetI ASST 8 cut(s) 33, 42, 54, 64, 161, 477, 565, 686
SfcI CTRYAG 1 cut(s) 347
SmiMI CAYNNNNRTG 1 cut(s) 390
Sse9I AATT 6 cut(s) 69, 197, 273, 409, 520, 619
SspMI CTAG 2 cut(s) 381, 575
StyD4I CCNGG 1 cut(s) 295
TaaI ACNGT 4 cut(s) 98, 359, 458, 630
TasI AATT 6 cut(s) 69, 197, 273, 409, 520, 619
TatI WGTACW 2 cut(s) 354, 359
TfiI GAWTC 1 cut(s) 436
Tru1I TTAA 1 cut(s) 536
Tru9I TTAA 1 cut(s) 536
TscAI CASTG 2 cut(s) 262, 347
TseFI GTSAC 2 cut(s) 279, 383
TseI GCWGC 2 cut(s) 346, 641
Tsp45I GTSAC 2 cut(s) 279, 383
TspDTI ATGAA 2 cut(s) 107, 650
TspRI CASTG 2 cut(s) 262, 347
XapI RAATTY 2 cut(s) 197, 520
XceI RCATGY 2 cut(s) 264, 471
XmiI GTMKAC 2 cut(s) 48, 625
XspI CTAG 2 cut(s) 381, 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.