FvH4_2g20150

monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010837
Physical Location & Seq
Reverse (-)
16922903 .. 16924928
2026 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g20150.t2

Sequence Viewer

Length: 1275 bp
ATGGAGAACTTATTTCGCTTAGTGGATCATGAGGATTTCTTTGCTCGCCGTTGCATCATGGTCAACGGGCCGGTCATAGTTGGAGCTGGCCCATCTGGCCTGGCAACGGCGGCTTGCCTGAGGGACCAAGGAGTCCCATTCGAAGTCCTTGAAAGAGCCGACTGCGTAGCCTCACTGTGGCAAAAACGCACCTATGACAGGCTCAAGCTTCATCTACCCAAGGCATTTTGCCAACTCCCTAAGCTTCCATTCCCTGAGGACTTCCCTGAGTACCCAACAAAGAGACAGTTCATCGACTACCTTGAATCATACGCCAAACACTTTGAAATCAACCCAAAGTTCAACTCCTGCGTCCAATCTGCCCGCTACGACGAGACCAGCGGTTTCTGGAGGGTCAACACAGTTTCAACAACCGGGTCGACCCGATCGGAGGTCGAGTACATCTGCAGGTGGCTCATAGTTGCCACCGGCGAAAATGCAGAGTGTGTGGTGCCCGATATCGATGGCTTGTGCGAGTTCGGCGGTGAAGTTGTCCACGCTTCTGAGTACAAGTCTGGCGAGAAGTACAAGGGAAAGAAAGTACTTGTCGTCGGTTGTGGAAATTCCGGCATGGAACTCTCTCTTGACCTCTGCAACCACAATGCCTCGCCTTCCATAGTCGTTAGAAGCTCGGTTCATGTCTTGCCTAGAGAAATCTACGGAAAATCGACATTCGAATTGGCTGTCTTTCTGCTAAAATGGCTACCAGTCTGGTTAGCTGACAAGCTTCTCTTGCTGTTTTCATGGTTGATACTCGGCAGCATTGAGAAATACGGGCTCAACAGGCCTTCCGTTGGACCAATGGAGCTAAAGAACACACAAGGAAAGACCCCTGTTCTTGACATTGGAGCACTAGACAAAATCAAGTCCGGTGGCATCAAAGTAGTCCACGGAATCAAGAGATTCTCATACGGACGCGTTGAACTTGTCGGCGGCGAAGTGCTCGACATTGATGCAGTTGTTTTAGCTACAGGATACCGCAGCAATGTTCCTTCTTGGCTACAGGAAGGTGATTTTTTCTCCAAGAATGGATTCCCAAAGCAGCCATTCCCACAGGGCTGGAAAGGAAATGCCGGACTCTACGCAGTCGGGTTCACAAGGAGAGGGCTCTCTGGTGCTTCATGTGACGCCGTGAGAATAGCACAAGATATTGGAAATGTCTGGAAATGTGAGACTAAGCAGAACAAGAAGAGAACCACAGCTGGTCACAGACGATGCATTTCCCAGACGTTCTAA

Protein Analysis

425

Amino Acids

47.22

Weight (kDa)

8.76

Isoelectric Point (pI)

42.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyr_redox_3 PF13738 25 - 224 2.1e-23 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox_2 PF07992 25 - 223 3.6e-10 Pyridine nucleotide-disulphide oxidoreductase
FMO-like PF00743 26 - 344 6.8e-29 Flavin-binding monooxygenase-like
Lys_Orn_oxgnase PF13434 90 - 220 2.8e-14 L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 438
AasI GACNNNNNNGTC 1 cut(s) 131
Acc36I ACCTGC 1 cut(s) 438
AccB1I GGYRCC 1 cut(s) 490
AccI GTMKAC 1 cut(s) 419
AccII CGCG 1 cut(s) 957
AciI CCGC 6 cut(s) 110, 364, 381, 522, 972, 1018
AclWI GGATC 1 cut(s) 33
AcsI RAATTY 1 cut(s) 601
AcyI GRCGYC 1 cut(s) 1167
AfaI GTAC 5 cut(s) 272, 440, 548, 566, 582
AfiI CCNNNNNNNGG 5 cut(s) 106, 177, 198, 430, 833
AflIII ACRYGT 1 cut(s) 955
AgsI TTSAA 6 cut(s) 152, 305, 326, 343, 408, 962
AjnI CCWGG 1 cut(s) 99
AjuI GAANNNNNNNTTGG 2 cut(s) 1055, 1087
AluBI AGCT 9 cut(s) 86, 208, 244, 669, 758, 766, 847, 1007, 1241
AluI AGCT 9 cut(s) 86, 208, 244, 669, 758, 766, 847, 1007, 1241
Alw21I GWGCWC 2 cut(s) 892, 984
Alw26I GTCTC 3 cut(s) 277, 368, 1205
AlwI GGATC 1 cut(s) 33
AoxI GGCC 4 cut(s) 68, 88, 97, 824
ApeKI GCWGC 3 cut(s) 798, 1020, 1081
ApoI RAATTY 1 cut(s) 601
ArsI GACNNNNNNTTYG 2 cut(s) 708, 740
Asp700I GAANNNNTTC 1 cut(s) 1070
AspS9I GGNCC 4 cut(s) 68, 89, 124, 836
AsuC2I CCSGG 1 cut(s) 415
AsuHPI GGTGA 2 cut(s) 536, 1061
AsuII TTCGAA 2 cut(s) 141, 714
AvaII GGWCC 2 cut(s) 124, 836
AxyI CCTNAGG 2 cut(s) 119, 255
BaeGI GKGCMC 1 cut(s) 495
BanI GGYRCC 1 cut(s) 490
BanII GRGCYC 2 cut(s) 819, 1149
Bbv12I GWGCWC 2 cut(s) 892, 984
BbvI GCAGC 3 cut(s) 810, 1032, 1093
BccI CCATC 2 cut(s) 100, 497
BceAI ACGGC 3 cut(s) 33, 123, 1154
BcgI CGANNNNNNTGC 2 cut(s) 974, 1008
BciT130I CCWGG 1 cut(s) 101
BciVI GTATCC 1 cut(s) 1007
BcnI CCSGG 1 cut(s) 415
BcoDI GTCTC 3 cut(s) 277, 368, 1205
BfaI CTAG 2 cut(s) 687, 893
BfmI CTRYAG 3 cut(s) 445, 1008, 1040
BfuAI ACCTGC 1 cut(s) 438
BfuI GTATCC 1 cut(s) 1007
BglI GCCNNNNNGGC 1 cut(s) 96
BisI GCNGC 5 cut(s) 111, 799, 973, 1021, 1082
BlsI GCNGC 5 cut(s) 112, 800, 974, 1022, 1083
BmcAI AGTACT 1 cut(s) 582
Bme1390I CCNGG 2 cut(s) 101, 415
Bme18I GGWCC 2 cut(s) 124, 836
BmgT120I GGNCC 4 cut(s) 68, 89, 124, 836
BmiI GGNNCC 2 cut(s) 125, 492
BmrFI CCNGG 2 cut(s) 101, 415
BmsI GCATC 4 cut(s) 63, 924, 982, 1244
BpmI CTGGAG 1 cut(s) 409
Bpu10I CCTNAGC 1 cut(s) 240
Bpu14I TTCGAA 2 cut(s) 141, 714
BpuEI CTTGAG 1 cut(s) 188
BpuMI CCSGG 1 cut(s) 415
Bsa29I ATCGAT 1 cut(s) 501
BsaHI GRCGYC 1 cut(s) 1167
BsaI GGTCTC 1 cut(s) 368
BsaJI CCNNGG 3 cut(s) 127, 219, 928
BsaWI WCCGGW 1 cut(s) 908
BsaXI ACNNNNNCTCC 2 cut(s) 422, 452
Bsc4I CCNNNNNNNGG 5 cut(s) 106, 177, 198, 430, 833
Bse118I RCCGGY 2 cut(s) 70, 467
Bse1I ACTGG 1 cut(s) 746
Bse21I CCTNAGG 2 cut(s) 119, 255
Bse3DI GCAATG 1 cut(s) 1030
BseBI CCWGG 1 cut(s) 101
BseCI ATCGAT 1 cut(s) 501
BseDI CCNNGG 3 cut(s) 127, 219, 928
BseLI CCNNNNNNNGG 5 cut(s) 106, 177, 198, 430, 833
BseMI GCAATG 1 cut(s) 1030
BseMII CTCAG 4 cut(s) 110, 246, 258, 534
BseNI ACTGG 1 cut(s) 746
BseSI GKGCMC 1 cut(s) 495
BseXI GCAGC 3 cut(s) 810, 1032, 1093
Bsh1236I CGCG 1 cut(s) 957
Bsh1285I CGRYCG 1 cut(s) 428
BshFI GGCC 4 cut(s) 70, 90, 99, 826
BshNI GGYRCC 1 cut(s) 490
BshVI ATCGAT 1 cut(s) 501
BsiEI CGRYCG 1 cut(s) 428
BsiHKAI GWGCWC 2 cut(s) 892, 984
BsiSI CCGG 6 cut(s) 71, 414, 468, 606, 909, 1113
BslFI GGGAC 2 cut(s) 119, 137
BslI CCNNNNNNNGG 5 cut(s) 106, 177, 198, 430, 833
BsmAI GTCTC 3 cut(s) 277, 368, 1205
BsmFI GGGAC 2 cut(s) 119, 137
BsnI GGCC 4 cut(s) 70, 90, 99, 826
Bso31I GGTCTC 1 cut(s) 368
Bsp119I TTCGAA 2 cut(s) 141, 714
Bsp1286I GDGCHC 5 cut(s) 495, 819, 892, 984, 1149
Bsp143I GATC 2 cut(s) 25, 425
BspACI CCGC 6 cut(s) 110, 364, 381, 522, 972, 1018
BspANI GGCC 4 cut(s) 70, 90, 99, 826
BspCNI CTCAG 4 cut(s) 111, 247, 259, 535
BspDI ATCGAT 1 cut(s) 501
BspFNI CGCG 1 cut(s) 957
BspHI TCATGA 1 cut(s) 28
BspLI GGNNCC 2 cut(s) 125, 492
BspMAI CTGCAG 1 cut(s) 449
BspMI ACCTGC 1 cut(s) 438
BspPI GGATC 1 cut(s) 33
BspT104I TTCGAA 2 cut(s) 141, 714
BspT107I GGYRCC 1 cut(s) 490
BspTNI GGTCTC 1 cut(s) 368
BsrDI GCAATG 1 cut(s) 1030
BsrFI RCCGGY 2 cut(s) 70, 467
BsrI ACTGG 1 cut(s) 746
BssAI RCCGGY 2 cut(s) 70, 467
BssECI CCNNGG 3 cut(s) 127, 219, 928
BssMI GATC 2 cut(s) 25, 425
BssNI GRCGYC 1 cut(s) 1167
BssT1I CCWWGG 2 cut(s) 127, 219
Bst2UI CCWGG 1 cut(s) 101
Bst4CI ACNGT 3 cut(s) 177, 288, 403
Bst6I CTCTTC 1 cut(s) 1223
BstACI GRCGYC 1 cut(s) 1167
BstBI TTCGAA 2 cut(s) 141, 714
BstC8I GCNNGC 4 cut(s) 46, 88, 115, 364
BstDEI CTNAG 7 cut(s) 19, 119, 240, 255, 267, 543, 1215
BstDSI CCRYGG 1 cut(s) 928
BstENI CCTNNNNNAGG 1 cut(s) 196
BstFNI CGCG 1 cut(s) 957
BstKTI GATC 2 cut(s) 28, 428
BstMAI GTCTC 3 cut(s) 277, 368, 1205
BstMBI GATC 2 cut(s) 25, 425
BstMCI CGRYCG 1 cut(s) 428
BstMWI GCNNNNNNNGC 6 cut(s) 96, 110, 519, 739, 772, 823
BstNI CCWGG 1 cut(s) 101
BstSCI CCNGG 2 cut(s) 99, 413
BstSFI CTRYAG 3 cut(s) 445, 1008, 1040
BstSLI GKGCMC 1 cut(s) 495
BstUI CGCG 1 cut(s) 957
BstV1I GCAGC 3 cut(s) 810, 1032, 1093
BstXI CCANNNNNNTGG 1 cut(s) 1098
Bsu15I ATCGAT 1 cut(s) 501
Bsu36I CCTNAGG 2 cut(s) 119, 255
BsuI GTATCC 1 cut(s) 1007
BsuRI GGCC 4 cut(s) 70, 90, 99, 826
BsuTUI ATCGAT 1 cut(s) 501
BtgI CCRYGG 1 cut(s) 928
BtsIMutI CAGTG 1 cut(s) 173
BveI ACCTGC 1 cut(s) 438
Cac8I GCNNGC 4 cut(s) 46, 88, 115, 364
CciI TCATGA 1 cut(s) 28
Cfr10I RCCGGY 2 cut(s) 70, 467
Cfr13I GGNCC 4 cut(s) 68, 89, 124, 836
ClaI ATCGAT 1 cut(s) 501
CseI GACGC 3 cut(s) 340, 963, 1175
Csp6I GTAC 5 cut(s) 271, 439, 547, 565, 581
CspCI CAANNNNNGTGG 2 cut(s) 892, 927
CviAII CATG 6 cut(s) 29, 58, 610, 677, 783, 1161
CviQI GTAC 5 cut(s) 271, 439, 547, 565, 581
DdeI CTNAG 7 cut(s) 19, 119, 240, 255, 267, 543, 1215
DpnI GATC 2 cut(s) 27, 427
DpnII GATC 2 cut(s) 25, 425
DrdI GACNNNNNNGTC 1 cut(s) 131
DseDI GACNNNNNNGTC 1 cut(s) 131
Eam1104I CTCTTC 1 cut(s) 1223
EarI CTCTTC 1 cut(s) 1223
Eco130I CCWWGG 2 cut(s) 127, 219
Eco147I AGGCCT 1 cut(s) 826
Eco24I GRGCYC 2 cut(s) 819, 1149
Eco31I GGTCTC 1 cut(s) 368
Eco32I GATATC 1 cut(s) 499
Eco47I GGWCC 2 cut(s) 124, 836
Eco81I CCTNAGG 2 cut(s) 119, 255
EcoNI CCTNNNNNAGG 1 cut(s) 196
EcoRII CCWGG 1 cut(s) 99
EcoRV GATATC 1 cut(s) 499
EcoT14I CCWWGG 2 cut(s) 127, 219
EcoT22I ATGCAT 1 cut(s) 1259
EcoT38I GRGCYC 2 cut(s) 819, 1149
ErhI CCWWGG 2 cut(s) 127, 219
FaeI CATG 6 cut(s) 32, 61, 613, 680, 786, 1164
FalI AAGNNNNNCTT 2 cut(s) 755, 787
FaqI GGGAC 2 cut(s) 119, 137
FatI CATG 6 cut(s) 28, 57, 609, 676, 782, 1160
FauI CCCGC 1 cut(s) 371
FblI GTMKAC 1 cut(s) 419
Fnu4HI GCNGC 5 cut(s) 111, 799, 973, 1021, 1082
FriOI GRGCYC 2 cut(s) 819, 1149
Fsp4HI GCNGC 5 cut(s) 111, 799, 973, 1021, 1082
FspBI CTAG 2 cut(s) 687, 893
GluI GCNGC 5 cut(s) 111, 799, 973, 1021, 1082
GsuI CTGGAG 1 cut(s) 409
HaeIII GGCC 4 cut(s) 70, 90, 99, 826
HapII CCGG 6 cut(s) 71, 414, 468, 606, 909, 1113
HgaI GACGC 3 cut(s) 340, 963, 1175
Hin1I GRCGYC 1 cut(s) 1167
Hin1II CATG 6 cut(s) 32, 61, 613, 680, 786, 1164
HincII GTYRAC 3 cut(s) 64, 397, 420
HindII GTYRAC 3 cut(s) 64, 397, 420
HindIII AAGCTT 3 cut(s) 206, 242, 764
HinfI GANTC 6 cut(s) 132, 305, 933, 942, 1071, 1116
HpaII CCGG 6 cut(s) 71, 414, 468, 606, 909, 1113
HphI GGTGA 2 cut(s) 536, 1061
Hpy166II GTNNAC 6 cut(s) 64, 397, 420, 535, 928, 1134
Hpy188I TCNGA 2 cut(s) 430, 544
Hpy188III TCNNGA 6 cut(s) 29, 388, 623, 878, 937, 1201
Hpy8I GTNNAC 6 cut(s) 64, 397, 420, 535, 928, 1134
Hpy99I CGWCG 2 cut(s) 374, 593
HpyAV CCTTC 4 cut(s) 660, 837, 1040, 1041
HpyCH4III ACNGT 3 cut(s) 177, 288, 403
HpyCH4IV ACGT 1 cut(s) 1268
HpyCH4V TGCA 6 cut(s) 54, 447, 479, 633, 995, 1257
HpyF10VI GCNNNNNNNGC 6 cut(s) 96, 110, 519, 739, 772, 823
HpyF3I CTNAG 7 cut(s) 19, 119, 240, 255, 267, 543, 1215
HpySE526I ACGT 1 cut(s) 1268
Hsp92I GRCGYC 1 cut(s) 1167
Hsp92II CATG 6 cut(s) 32, 61, 613, 680, 786, 1164
Kzo9I GATC 2 cut(s) 25, 425
LmnI GCTCC 3 cut(s) 83, 844, 887
Lsp1109I GCAGC 3 cut(s) 810, 1032, 1093
LweI GCATC 4 cut(s) 63, 924, 982, 1244
MaeI CTAG 2 cut(s) 687, 893
MaeII ACGT 1 cut(s) 1268
MaeIII GTNAC 2 cut(s) 1163, 1244
MalI GATC 2 cut(s) 27, 427
MboI GATC 2 cut(s) 25, 425
MboII GAAGA 1 cut(s) 1240
MhlI GDGCHC 5 cut(s) 495, 819, 892, 984, 1149
MluCI AATT 2 cut(s) 601, 716
MluI ACGCGT 1 cut(s) 955
MlyI GAGTC 2 cut(s) 141, 1110
MmeI TCCRAC 2 cut(s) 61, 814
MnlI CCTC 9 cut(s) 25, 114, 181, 250, 384, 424, 638, 655, 1136
Mph1103I ATGCAT 1 cut(s) 1259
MroXI GAANNNNTTC 1 cut(s) 1070
MspA1I CMGCKG 2 cut(s) 381, 1241
MspI CCGG 6 cut(s) 71, 414, 468, 606, 909, 1113
MspR9I CCNGG 2 cut(s) 101, 415
MvaI CCWGG 1 cut(s) 101
MvnI CGCG 1 cut(s) 957
MwoI GCNNNNNNNGC 6 cut(s) 96, 110, 519, 739, 772, 823
NciI CCSGG 1 cut(s) 415
NdeII GATC 2 cut(s) 25, 425
NlaIII CATG 6 cut(s) 32, 61, 613, 680, 786, 1164
NlaIV GGNNCC 2 cut(s) 125, 492
NmeAIII GCCGAG 1 cut(s) 774
NmuCI GTSAC 2 cut(s) 1163, 1244
NsiI ATGCAT 1 cut(s) 1259
NspV TTCGAA 2 cut(s) 141, 714
PagI TCATGA 1 cut(s) 28
PaqCI CACCTGC 1 cut(s) 438
PceI AGGCCT 1 cut(s) 826
PdmI GAANNNNTTC 1 cut(s) 1070
PfeI GAWTC 4 cut(s) 305, 933, 942, 1071
PkrI GCNGC 5 cut(s) 112, 800, 974, 1022, 1083
Ple19I CGATCG 1 cut(s) 428
PleI GAGTC 2 cut(s) 140, 1110
PpsI GAGTC 2 cut(s) 140, 1110
Psp6I CCWGG 1 cut(s) 99
PspGI CCWGG 1 cut(s) 99
PspN4I GGNNCC 2 cut(s) 125, 492
PspPI GGNCC 4 cut(s) 68, 89, 124, 836
PstI CTGCAG 1 cut(s) 449
PvuI CGATCG 1 cut(s) 428
PvuII CAGCTG 1 cut(s) 1241
RsaI GTAC 5 cut(s) 272, 440, 548, 566, 582
RsaNI GTAC 5 cut(s) 271, 439, 547, 565, 581
SalI GTCGAC 1 cut(s) 418
SatI GCNGC 5 cut(s) 111, 799, 973, 1021, 1082
Sau3AI GATC 2 cut(s) 25, 425
Sau96I GGNCC 4 cut(s) 68, 89, 124, 836
ScaI AGTACT 1 cut(s) 582
SchI GAGTC 2 cut(s) 141, 1110
ScrFI CCNGG 2 cut(s) 101, 415
SduI GDGCHC 5 cut(s) 495, 819, 892, 984, 1149
SfaNI GCATC 4 cut(s) 63, 924, 982, 1244
SfcI CTRYAG 3 cut(s) 445, 1008, 1040
SfiI GGCCNNNNNGGCC 1 cut(s) 96
SfuI TTCGAA 2 cut(s) 141, 714
SgrAI CRCCGGYG 1 cut(s) 467
SinI GGWCC 2 cut(s) 124, 836
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
Sse9I AATT 2 cut(s) 601, 716
SseBI AGGCCT 1 cut(s) 826
SsiI CCGC 6 cut(s) 110, 364, 381, 522, 972, 1018
SspMI CTAG 2 cut(s) 687, 893
StuI AGGCCT 1 cut(s) 826
StyD4I CCNGG 2 cut(s) 99, 413
StyI CCWWGG 2 cut(s) 127, 219
TaaI ACNGT 3 cut(s) 177, 288, 403
TaiI ACGT 1 cut(s) 1271
TaqI TCGA 8 cut(s) 141, 294, 419, 435, 501, 707, 714, 984
TasI AATT 2 cut(s) 601, 716
TatI WGTACW 4 cut(s) 438, 546, 564, 580
TauI GCSGC 2 cut(s) 113, 975
TfiI GAWTC 4 cut(s) 305, 933, 942, 1071
TscAI CASTG 1 cut(s) 180
TseFI GTSAC 2 cut(s) 1163, 1244
TseI GCWGC 3 cut(s) 798, 1020, 1081
Tsp45I GTSAC 2 cut(s) 1163, 1244
TspDTI ATGAA 5 cut(s) 200, 280, 665, 771, 1149
TspGWI ACGGA 4 cut(s) 714, 820, 945, 966
TspRI CASTG 1 cut(s) 180
VpaK11BI GGWCC 2 cut(s) 124, 836
XagI CCTNNNNNAGG 1 cut(s) 196
XapI RAATTY 1 cut(s) 601
XmiI GTMKAC 1 cut(s) 419
XmnI GAANNNNTTC 1 cut(s) 1070
XspI CTAG 2 cut(s) 687, 893
ZrmI AGTACT 1 cut(s) 582
Zsp2I ATGCAT 1 cut(s) 1259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.