RchiOBHm_Chr6g0285851

monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0010837
Physical Location & Seq
Reverse (-)
49112417 .. 49114114
1698 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25643

Sequence Viewer

Length: 1275 bp
ATGGAGAACTTGTTTCGCTTAGTGGATCATGAGGATTTCTTTGCTCGCCGTTGCATCATGGTCAACGGACCAGTCATAGTTGGAGCTGGTCCATCTGGCTTGGCAACGGCAGCTTGCCTCAGGGATCAAGGAGTCCCATTCGAAGTCCTTGAAAGAGCCGAGTGCATAGCCTCACTGTGGCAAAAACGCACCTATGACAGGCTCAAGCTTCATCTTCCCAAGGCATTTTGCCAACTCCCTAAACTTCCATTCCCTCAGGATTTCCCTGAGTATCCAACAAAGAGACAATTCATCGACTACCTTGAATCATACGCCAAACACTTTGAAATCAACCCAAAATTCAACTCCTCCGTCCAATCAGCCCGCTACGACGAGACCAGCGGTTTCTGGAGGGTCAAGACAGTTTCAACAACCGGGTCAACCCGATCAGAGGTCGAGTACATCTGCAGGTGGCTCATAGTTGCCACCGGCGAAAATGCCGAGTGTGTGGTGCCTGACATCGACGGACTCAGCGAGTTTGGCGGCGAAGTTGTCCACGCTTGTGAGTACAAGTCCGGCGAGAAGTACAAGGGACAGAAAGTACTTGTCGTCGGTTGTGGAAATTCCGGCATGGAACTCTCTCTTGACCTCTGCAACCACAATGCCTCCCCTTCCATGGTCGTTAGAAGCTCGGTTCATGTCTTGCCAAGAGAAATCTATGGAAAATCGACATTCGAGTTGGCGGTCTTTTTGCTAAAATGGCTACCAGTCTGGTTAGCTGACAAGCTCCTCCTACTTCTCTCATGGTTGATACTCGGAAGCATCGAGAAATACGGGCTCAACAGGCCTTCCGTAGGCCCATTGGAGCTAAAGAACACACAAGGAAAGACCCCTGTTCTTGACATTGGAGCACTAGACAAAATCAAGTCCGGTGGTATCAAAGTCGTTCCCGGAATCAAGAGATTCTCATACGGCCGCGTTGAGCTTGTCGACGGCGAAGTTCTTGACATTGATTCAGTTGTTCTAGCTACAGGATACCGCAGCAATGTTCCTTCTTGGCTACAGGAAGGTGAATTTTTCTCCAAGAACGGATTCCCAAAGCAGCCATTCCCACAGGGCTGGAAAGGAAATGCCGGACTCTATGCAGTCGGGTTCACAAGGAGAGGGCTCTCTGGTGCTTCATGTGACGCCATGAGAATAGCACAAGATATTGGAAACGTCTGGAAATGCGAAACGAAACAGAACAAGAAGAGAACCACCGCTGGCCATAGACGATGCATTTCCCAGACGTTCTAA

Protein Analysis

424

Amino Acids

47.29

Weight (kDa)

8.79

Isoelectric Point (pI)

45.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyr_redox_3 PF13738 25 - 224 7e-24 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox_2 PF07992 25 - 222 1.4e-10 Pyridine nucleotide-disulphide oxidoreductase
FMO-like PF00743 26 - 344 1.6e-28 Flavin-binding monooxygenase-like
Lys_Orn_oxgnase PF13434 90 - 218 1.9e-13 L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 438
Acc36I ACCTGC 1 cut(s) 438
AccB1I GGYRCC 1 cut(s) 490
AccI GTMKAC 1 cut(s) 969
AccII CGCG 1 cut(s) 957
AciI CCGC 7 cut(s) 364, 381, 522, 722, 955, 1018, 1239
AclWI GGATC 2 cut(s) 33, 132
AcoI YGGCCR 2 cut(s) 952, 1243
AcsI RAATTY 3 cut(s) 338, 601, 1052
AcyI GRCGYC 1 cut(s) 1167
AfaI GTAC 4 cut(s) 440, 548, 566, 582
AfiI CCNNNNNNNGG 5 cut(s) 177, 198, 430, 655, 833
AgsI TTSAA 5 cut(s) 152, 305, 326, 343, 408
AjuI GAANNNNNNNTTGG 2 cut(s) 1055, 1087
AleI CACNNNNGTG 1 cut(s) 540
AluBI AGCT 9 cut(s) 86, 113, 208, 669, 758, 766, 847, 964, 1007
AluI AGCT 9 cut(s) 86, 113, 208, 669, 758, 766, 847, 964, 1007
Alw21I GWGCWC 1 cut(s) 892
Alw26I GTCTC 2 cut(s) 277, 368
AlwI GGATC 2 cut(s) 33, 132
AoxI GGCC 4 cut(s) 824, 835, 952, 1243
ApeKI GCWGC 3 cut(s) 110, 1020, 1081
ApoI RAATTY 3 cut(s) 338, 601, 1052
Asp700I GAANNNNTTC 1 cut(s) 1070
AspS9I GGNCC 3 cut(s) 68, 89, 836
AsuC2I CCSGG 2 cut(s) 415, 930
AsuHPI GGTGA 1 cut(s) 1061
AsuII TTCGAA 1 cut(s) 141
AvaII GGWCC 2 cut(s) 68, 89
AxyI CCTNAGG 2 cut(s) 119, 255
BalI TGGCCA 1 cut(s) 1245
BanI GGYRCC 1 cut(s) 490
BanII GRGCYC 2 cut(s) 819, 1149
Bbv12I GWGCWC 1 cut(s) 892
BbvI GCAGC 3 cut(s) 122, 1032, 1093
BccI CCATC 1 cut(s) 100
BceAI ACGGC 4 cut(s) 33, 123, 967, 988
BciVI GTATCC 2 cut(s) 282, 1007
BcnI CCSGG 2 cut(s) 415, 930
BcoDI GTCTC 2 cut(s) 277, 368
BfaI CTAG 2 cut(s) 893, 1004
BfmI CTRYAG 3 cut(s) 445, 1008, 1040
BfuAI ACCTGC 1 cut(s) 438
BfuI GTATCC 2 cut(s) 282, 1007
BisI GCNGC 5 cut(s) 111, 523, 955, 1021, 1082
BlsI GCNGC 5 cut(s) 112, 524, 956, 1022, 1083
BmcAI AGTACT 1 cut(s) 582
Bme1390I CCNGG 2 cut(s) 415, 930
Bme18I GGWCC 2 cut(s) 68, 89
BmgT120I GGNCC 3 cut(s) 68, 89, 836
BmiI GGNNCC 1 cut(s) 492
BmrFI CCNGG 2 cut(s) 415, 930
BmsI GCATC 3 cut(s) 63, 810, 1244
BpmI CTGGAG 1 cut(s) 409
Bpu14I TTCGAA 1 cut(s) 141
BpuEI CTTGAG 1 cut(s) 188
BpuMI CCSGG 2 cut(s) 415, 930
BsaHI GRCGYC 1 cut(s) 1167
BsaI GGTCTC 1 cut(s) 368
BsaJI CCNNGG 2 cut(s) 219, 654
BsaWI WCCGGW 1 cut(s) 908
BsaXI ACNNNNNCTCC 3 cut(s) 26, 629, 659
Bsc4I CCNNNNNNNGG 5 cut(s) 177, 198, 430, 655, 833
Bse118I RCCGGY 1 cut(s) 467
Bse1I ACTGG 2 cut(s) 71, 746
Bse21I CCTNAGG 2 cut(s) 119, 255
Bse3DI GCAATG 1 cut(s) 1030
BseDI CCNNGG 2 cut(s) 219, 654
BseLI CCNNNNNNNGG 5 cut(s) 177, 198, 430, 655, 833
BseMI GCAATG 1 cut(s) 1030
BseMII CTCAG 4 cut(s) 133, 258, 269, 523
BseNI ACTGG 2 cut(s) 71, 746
BseRI GAGGAG 2 cut(s) 337, 758
BseX3I CGGCCG 1 cut(s) 952
BseXI GCAGC 3 cut(s) 122, 1032, 1093
Bsh1236I CGCG 1 cut(s) 957
Bsh1285I CGRYCG 1 cut(s) 955
BshFI GGCC 4 cut(s) 826, 837, 954, 1245
BshNI GGYRCC 1 cut(s) 490
BsiEI CGRYCG 1 cut(s) 955
BsiHKAI GWGCWC 1 cut(s) 892
BsiSI CCGG 7 cut(s) 414, 468, 555, 606, 909, 930, 1113
BslFI GGGAC 2 cut(s) 119, 585
BslI CCNNNNNNNGG 5 cut(s) 177, 198, 430, 655, 833
BsmAI GTCTC 2 cut(s) 277, 368
BsmFI GGGAC 2 cut(s) 119, 585
BsnI GGCC 4 cut(s) 826, 837, 954, 1245
Bso31I GGTCTC 1 cut(s) 368
Bsp119I TTCGAA 1 cut(s) 141
Bsp1286I GDGCHC 3 cut(s) 819, 892, 1149
Bsp143I GATC 3 cut(s) 25, 124, 425
Bsp19I CCATGG 1 cut(s) 654
BspACI CCGC 7 cut(s) 364, 381, 522, 722, 955, 1018, 1239
BspANI GGCC 4 cut(s) 826, 837, 954, 1245
BspCNI CTCAG 4 cut(s) 132, 259, 268, 522
BspFNI CGCG 1 cut(s) 957
BspHI TCATGA 1 cut(s) 28
BspLI GGNNCC 1 cut(s) 492
BspMAI CTGCAG 1 cut(s) 449
BspMI ACCTGC 1 cut(s) 438
BspPI GGATC 2 cut(s) 33, 132
BspT104I TTCGAA 1 cut(s) 141
BspT107I GGYRCC 1 cut(s) 490
BspTNI GGTCTC 1 cut(s) 368
BsrDI GCAATG 1 cut(s) 1030
BsrFI RCCGGY 1 cut(s) 467
BsrI ACTGG 2 cut(s) 71, 746
BssAI RCCGGY 1 cut(s) 467
BssECI CCNNGG 2 cut(s) 219, 654
BssMI GATC 3 cut(s) 25, 124, 425
BssNI GRCGYC 1 cut(s) 1167
BssT1I CCWWGG 2 cut(s) 219, 654
Bst4CI ACNGT 2 cut(s) 177, 403
Bst6I CTCTTC 1 cut(s) 1223
BstACI GRCGYC 1 cut(s) 1167
BstBI TTCGAA 1 cut(s) 141
BstC8I GCNNGC 4 cut(s) 46, 115, 364, 1243
BstDEI CTNAG 5 cut(s) 19, 119, 255, 267, 509
BstDSI CCRYGG 1 cut(s) 654
BstENI CCTNNNNNAGG 2 cut(s) 196, 831
BstFNI CGCG 1 cut(s) 957
BstKTI GATC 3 cut(s) 28, 127, 428
BstMAI GTCTC 2 cut(s) 277, 368
BstMBI GATC 3 cut(s) 25, 124, 425
BstMCI CGRYCG 1 cut(s) 955
BstMWI GCNNNNNNNGC 4 cut(s) 110, 519, 739, 823
BstSCI CCNGG 2 cut(s) 413, 928
BstSFI CTRYAG 3 cut(s) 445, 1008, 1040
BstUI CGCG 1 cut(s) 957
BstV1I GCAGC 3 cut(s) 122, 1032, 1093
BstXI CCANNNNNNTGG 1 cut(s) 1098
BstZI CGGCCG 1 cut(s) 952
Bsu36I CCTNAGG 2 cut(s) 119, 255
BsuI GTATCC 2 cut(s) 282, 1007
BsuRI GGCC 4 cut(s) 826, 837, 954, 1245
BtgI CCRYGG 1 cut(s) 654
BtsIMutI CAGTG 1 cut(s) 173
BveI ACCTGC 1 cut(s) 438
Cac8I GCNNGC 4 cut(s) 46, 115, 364, 1243
CciI TCATGA 1 cut(s) 28
Cfr10I RCCGGY 1 cut(s) 467
Cfr13I GGNCC 3 cut(s) 68, 89, 836
CseI GACGC 1 cut(s) 1175
Csp6I GTAC 4 cut(s) 439, 547, 565, 581
CspCI CAANNNNNGTGG 2 cut(s) 892, 927
CviAII CATG 8 cut(s) 29, 58, 610, 655, 677, 783, 1161, 1171
CviQI GTAC 4 cut(s) 439, 547, 565, 581
DdeI CTNAG 5 cut(s) 19, 119, 255, 267, 509
DpnI GATC 3 cut(s) 27, 126, 427
DpnII GATC 3 cut(s) 25, 124, 425
EaeI YGGCCR 2 cut(s) 952, 1243
EagI CGGCCG 1 cut(s) 952
Eam1104I CTCTTC 1 cut(s) 1223
EarI CTCTTC 1 cut(s) 1223
EclXI CGGCCG 1 cut(s) 952
Eco130I CCWWGG 2 cut(s) 219, 654
Eco147I AGGCCT 1 cut(s) 826
Eco24I GRGCYC 2 cut(s) 819, 1149
Eco31I GGTCTC 1 cut(s) 368
Eco47I GGWCC 2 cut(s) 68, 89
Eco52I CGGCCG 1 cut(s) 952
Eco81I CCTNAGG 2 cut(s) 119, 255
EcoNI CCTNNNNNAGG 2 cut(s) 196, 831
EcoT14I CCWWGG 2 cut(s) 219, 654
EcoT22I ATGCAT 1 cut(s) 1259
EcoT38I GRGCYC 2 cut(s) 819, 1149
ErhI CCWWGG 2 cut(s) 219, 654
FaeI CATG 8 cut(s) 32, 61, 613, 658, 680, 786, 1164, 1174
FaqI GGGAC 2 cut(s) 119, 585
FatI CATG 8 cut(s) 28, 57, 609, 654, 676, 782, 1160, 1170
FauI CCCGC 1 cut(s) 371
FblI GTMKAC 1 cut(s) 969
Fnu4HI GCNGC 5 cut(s) 111, 523, 955, 1021, 1082
FriOI GRGCYC 2 cut(s) 819, 1149
Fsp4HI GCNGC 5 cut(s) 111, 523, 955, 1021, 1082
FspBI CTAG 2 cut(s) 893, 1004
GluI GCNGC 5 cut(s) 111, 523, 955, 1021, 1082
GsuI CTGGAG 1 cut(s) 409
HaeIII GGCC 4 cut(s) 826, 837, 954, 1245
HapII CCGG 7 cut(s) 414, 468, 555, 606, 909, 930, 1113
HgaI GACGC 1 cut(s) 1175
Hin1I GRCGYC 1 cut(s) 1167
Hin1II CATG 8 cut(s) 32, 61, 613, 658, 680, 786, 1164, 1174
HincII GTYRAC 3 cut(s) 64, 420, 970
HindII GTYRAC 3 cut(s) 64, 420, 970
HindIII AAGCTT 1 cut(s) 206
HinfI GANTC 8 cut(s) 132, 305, 507, 933, 942, 992, 1071, 1116
HpaII CCGG 7 cut(s) 414, 468, 555, 606, 909, 930, 1113
HphI GGTGA 1 cut(s) 1061
Hpy166II GTNNAC 5 cut(s) 64, 420, 535, 970, 1134
Hpy188I TCNGA 2 cut(s) 430, 797
Hpy8I GTNNAC 5 cut(s) 64, 420, 535, 970, 1134
Hpy99I CGWCG 4 cut(s) 374, 506, 593, 974
HpyAV CCTTC 4 cut(s) 660, 837, 1040, 1041
HpyCH4III ACNGT 2 cut(s) 177, 403
HpyCH4IV ACGT 2 cut(s) 1197, 1268
HpyCH4V TGCA 6 cut(s) 54, 165, 447, 633, 1124, 1257
HpyF10VI GCNNNNNNNGC 4 cut(s) 110, 519, 739, 823
HpyF3I CTNAG 5 cut(s) 19, 119, 255, 267, 509
HpySE526I ACGT 2 cut(s) 1197, 1268
Hsp92I GRCGYC 1 cut(s) 1167
Hsp92II CATG 8 cut(s) 32, 61, 613, 658, 680, 786, 1164, 1174
Kzo9I GATC 3 cut(s) 25, 124, 425
LmnI GCTCC 4 cut(s) 83, 771, 844, 887
Lsp1109I GCAGC 3 cut(s) 122, 1032, 1093
LweI GCATC 3 cut(s) 63, 810, 1244
MaeI CTAG 2 cut(s) 893, 1004
MaeII ACGT 2 cut(s) 1197, 1268
MaeIII GTNAC 1 cut(s) 1163
MalI GATC 3 cut(s) 27, 126, 427
MboI GATC 3 cut(s) 25, 124, 425
MboII GAAGA 2 cut(s) 206, 1240
MhlI GDGCHC 3 cut(s) 819, 892, 1149
MlsI TGGCCA 1 cut(s) 1245
MluCI AATT 4 cut(s) 287, 338, 601, 1052
MluNI TGGCCA 1 cut(s) 1245
MlyI GAGTC 3 cut(s) 141, 501, 1110
MmeI TCCRAC 2 cut(s) 61, 299
Mox20I TGGCCA 1 cut(s) 1245
Mph1103I ATGCAT 1 cut(s) 1259
MroXI GAANNNNTTC 1 cut(s) 1070
MscI TGGCCA 1 cut(s) 1245
MslI CAYNNNNRTG 1 cut(s) 540
Msp20I TGGCCA 1 cut(s) 1245
MspA1I CMGCKG 2 cut(s) 381, 1241
MspI CCGG 7 cut(s) 414, 468, 555, 606, 909, 930, 1113
MspR9I CCNGG 2 cut(s) 415, 930
MvnI CGCG 1 cut(s) 957
MwoI GCNNNNNNNGC 4 cut(s) 110, 519, 739, 823
NciI CCSGG 2 cut(s) 415, 930
NcoI CCATGG 1 cut(s) 654
NdeII GATC 3 cut(s) 25, 124, 425
NlaIII CATG 8 cut(s) 32, 61, 613, 658, 680, 786, 1164, 1174
NlaIV GGNNCC 1 cut(s) 492
NmeAIII GCCGAG 2 cut(s) 184, 505
NmuCI GTSAC 1 cut(s) 1163
NsiI ATGCAT 1 cut(s) 1259
NspV TTCGAA 1 cut(s) 141
OliI CACNNNNGTG 1 cut(s) 540
PagI TCATGA 1 cut(s) 28
PaqCI CACCTGC 1 cut(s) 438
PceI AGGCCT 1 cut(s) 826
PcsI WCGNNNNNNNCGW 2 cut(s) 510, 801
PdmI GAANNNNTTC 1 cut(s) 1070
PfeI GAWTC 5 cut(s) 305, 933, 942, 992, 1071
PfoI TCCNGGA 1 cut(s) 928
PkrI GCNGC 5 cut(s) 112, 524, 956, 1022, 1083
PleI GAGTC 3 cut(s) 140, 501, 1110
PpsI GAGTC 3 cut(s) 140, 501, 1110
PspN4I GGNNCC 1 cut(s) 492
PspPI GGNCC 3 cut(s) 68, 89, 836
PstI CTGCAG 1 cut(s) 449
RsaI GTAC 4 cut(s) 440, 548, 566, 582
RsaNI GTAC 4 cut(s) 439, 547, 565, 581
RseI CAYNNNNRTG 1 cut(s) 540
SalI GTCGAC 1 cut(s) 968
SatI GCNGC 5 cut(s) 111, 523, 955, 1021, 1082
Sau3AI GATC 3 cut(s) 25, 124, 425
Sau96I GGNCC 3 cut(s) 68, 89, 836
ScaI AGTACT 1 cut(s) 582
SchI GAGTC 3 cut(s) 141, 501, 1110
ScrFI CCNGG 2 cut(s) 415, 930
SduI GDGCHC 3 cut(s) 819, 892, 1149
SfaNI GCATC 3 cut(s) 63, 810, 1244
SfcI CTRYAG 3 cut(s) 445, 1008, 1040
SfuI TTCGAA 1 cut(s) 141
SgrAI CRCCGGYG 1 cut(s) 467
SinI GGWCC 2 cut(s) 68, 89
SmiMI CAYNNNNRTG 1 cut(s) 540
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
Sse9I AATT 4 cut(s) 287, 338, 601, 1052
SseBI AGGCCT 1 cut(s) 826
SsiI CCGC 7 cut(s) 364, 381, 522, 722, 955, 1018, 1239
SspMI CTAG 2 cut(s) 893, 1004
StuI AGGCCT 1 cut(s) 826
StyD4I CCNGG 2 cut(s) 413, 928
StyI CCWWGG 2 cut(s) 219, 654
TaaI ACNGT 2 cut(s) 177, 403
TaiI ACGT 2 cut(s) 1200, 1271
TaqI TCGA 8 cut(s) 141, 294, 435, 501, 707, 714, 804, 969
TasI AATT 4 cut(s) 287, 338, 601, 1052
TatI WGTACW 4 cut(s) 438, 546, 564, 580
TauI GCSGC 2 cut(s) 525, 957
TfiI GAWTC 5 cut(s) 305, 933, 942, 992, 1071
TscAI CASTG 1 cut(s) 180
TseFI GTSAC 1 cut(s) 1163
TseI GCWGC 3 cut(s) 110, 1020, 1081
Tsp45I GTSAC 1 cut(s) 1163
TspDTI ATGAA 4 cut(s) 200, 280, 665, 1149
TspGWI ACGGA 5 cut(s) 81, 340, 519, 820, 1083
TspRI CASTG 1 cut(s) 180
VpaK11BI GGWCC 2 cut(s) 68, 89
XagI CCTNNNNNAGG 2 cut(s) 196, 831
XapI RAATTY 3 cut(s) 338, 601, 1052
XmiI GTMKAC 1 cut(s) 969
XmnI GAANNNNTTC 1 cut(s) 1070
XspI CTAG 2 cut(s) 893, 1004
ZrmI AGTACT 1 cut(s) 582
Zsp2I ATGCAT 1 cut(s) 1259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.