FvH4_2g20530

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012577
Physical Location & Seq
Forward (+)
17216123 .. 17217960
1838 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g20530.t4

Sequence Viewer

Length: 1086 bp
ATGTTGCTAAGAAGCTCTTCAACACCAGTCATTGGATCCCTTCTTGCATCATTCTCAGCCGAGAGTCCACTTGGCAATCACTTTGAGCCAAAACCAAGCTCAATCCACAGCACACCCAATAAGTTTTCATTTCACACACCTGGGCCTCTCAACATCTCACACAGTTCCTCACCGATCTCTCCCTCCATTGATCTTTCCTCCGGGTTTCTCCGGAGGGCTCAGTCTGATGGGAACTTGGAAGGTCTGGCATATGCTGCGTCTTGTGAGAAAAATGAAGATGATCCCTTCTTCTGTGATTTGTACCAACACAACAACTCGAGATCCAGGGGCATAACATTGCAGACTATACAGTCATTTTCGACATATAACTCGGCTGATTTGGGTGAAGATGATGATGAAGAAGATAGTGATAGGGAATATGATGAAGAGGAAGTAGAAGAGGTTGAGGCTATTGGAGGTGATAATGTTGGTTTGGAAGAGAAGGTGAAGAAGGTGAGTTTATCTGAGGATATGATAACTATGCAGAAAATGTGGGGCGGGAGTTTTGAAGAACAGAGAGGTCAAGTAAGTCAAGAAATGCATCTTGCAAAGGGACTTGGGATTGATGGCGGAAGTGGCGGCGGCCGGGGCGGTCGTGAGGCTAAATGGAAAAGCAGTGGTGGAGATGGAGGTGACAACAACAAGGGTGTGGAGGAGCATTACAAGAAGATGGTGGAGGAACATCCGGGAAACCCTTTGTTTCTAGGAAATTATGCTAATTTCTTGTATGAGACCAAGAGAGACCTTCCTTTAGCAGAGGAATACTACTCGCGTTCGATCCTAGCAGACCCGAATGATGGTGAAATTTTGGCACAGTACGCCAAGGTTATATGGGAGTTGCACCATGATGAAGATAGAGCCTTAAGCTACTATGAACGAGCAGTCCAAGCTGCACCTCAAGACAGCTTTGTCCATGCAGCATATGCTAGCTTCCTATGGACAACAGAGGAAGACGATGAAGATGAAGATGAAGCAGATCGGCGACAAGATCTTGGCGCCATGTCGCCTTATATTCATGGAGGAATCATGACTTCTGCAAGTGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_19

Protein Analysis

362

Amino Acids

39.79

Weight (kDa)

4.51

Isoelectric Point (pI)

66.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 349, 947
AccB1I GGYRCC 1 cut(s) 1034
AccB7I CCANNNNNTGG 1 cut(s) 32
AccII CGCG 1 cut(s) 811
AccIII TCCGGA 1 cut(s) 210
AciI CCGC 5 cut(s) 537, 609, 618, 621, 630
AclWI GGATC 5 cut(s) 30, 43, 275, 315, 811
AcoI YGGCCR 1 cut(s) 622
AcsI RAATTY 1 cut(s) 843
AcyI GRCGYC 1 cut(s) 1035
AfaI GTAC 2 cut(s) 302, 857
AfiI CCNNNNNNNGG 2 cut(s) 32, 836
AflII CTTAAG 1 cut(s) 901
AgsI TTSAA 2 cut(s) 21, 548
AjnI CCWGG 2 cut(s) 139, 323
AloI GAACNNNNNNTCC 2 cut(s) 906, 938
AluBI AGCT 6 cut(s) 15, 99, 906, 929, 945, 969
AluI AGCT 6 cut(s) 15, 99, 906, 929, 945, 969
Alw26I GTCTC 2 cut(s) 764, 774
AlwI GGATC 5 cut(s) 30, 43, 275, 315, 811
Ama87I CYCGRG 1 cut(s) 316
Aor13HI TCCGGA 1 cut(s) 210
AoxI GGCC 2 cut(s) 143, 622
ApeKI GCWGC 3 cut(s) 254, 929, 956
ApoI RAATTY 1 cut(s) 843
Asp700I GAANNNNTTC 1 cut(s) 16
AspLEI GCGC 1 cut(s) 1037
AspS9I GGNCC 1 cut(s) 143
AsuC2I CCSGG 3 cut(s) 202, 626, 726
AsuHPI GGTGA 7 cut(s) 162, 395, 470, 496, 505, 683, 851
AsuNHI GCTAGC 1 cut(s) 965
AvaI CYCGRG 1 cut(s) 316
BamHI GGATCC 1 cut(s) 35
BanI GGYRCC 1 cut(s) 1034
BanII GRGCYC 1 cut(s) 220
BbsI GAAGAC 1 cut(s) 996
BbvI GCAGC 3 cut(s) 241, 916, 968
BccI CCATC 5 cut(s) 221, 599, 659, 703, 830
BciT130I CCWGG 2 cut(s) 141, 325
BcnI CCSGG 3 cut(s) 202, 626, 726
BcoDI GTCTC 2 cut(s) 764, 774
BfaI CTAG 3 cut(s) 743, 821, 966
BfoI RGCGCY 1 cut(s) 1038
BfrI CTTAAG 1 cut(s) 901
BglII AGATCT 1 cut(s) 1027
BisI GCNGC 5 cut(s) 255, 619, 622, 930, 957
BlsI GCNGC 5 cut(s) 256, 620, 623, 931, 958
Bme1390I CCNGG 5 cut(s) 141, 202, 325, 626, 726
BmeT110I CYCGRG 1 cut(s) 316
BmgT120I GGNCC 1 cut(s) 143
BmiI GGNNCC 2 cut(s) 37, 1036
BmrFI CCNGG 5 cut(s) 141, 202, 325, 626, 726
BmsI GCATC 2 cut(s) 56, 589
BmtI GCTAGC 1 cut(s) 969
BpiI GAAGAC 1 cut(s) 996
BpuEI CTTGAG 1 cut(s) 921
BpuMI CCSGG 3 cut(s) 202, 626, 726
BsaHI GRCGYC 1 cut(s) 1035
BsaI GGTCTC 2 cut(s) 764, 774
BsaJI CCNNGG 4 cut(s) 140, 324, 625, 861
BsaWI WCCGGW 1 cut(s) 210
Bsc4I CCNNNNNNNGG 2 cut(s) 32, 836
Bse1I ACTGG 1 cut(s) 26
Bse3DI GCAATG 1 cut(s) 335
BseAI TCCGGA 1 cut(s) 210
BseBI CCWGG 2 cut(s) 141, 325
BseDI CCNNGG 4 cut(s) 140, 324, 625, 861
BseGI GGATG 1 cut(s) 721
BseLI CCNNNNNNNGG 2 cut(s) 32, 836
BseMI GCAATG 1 cut(s) 335
BseMII CTCAG 3 cut(s) 69, 233, 495
BseNI ACTGG 1 cut(s) 26
BseRI GAGGAG 1 cut(s) 707
BseX3I CGGCCG 1 cut(s) 622
BseXI GCAGC 3 cut(s) 241, 916, 968
BsgI GTGCAG 1 cut(s) 915
Bsh1236I CGCG 1 cut(s) 811
Bsh1285I CGRYCG 2 cut(s) 625, 634
BshFI GGCC 2 cut(s) 145, 624
BshNI GGYRCC 1 cut(s) 1034
BsiEI CGRYCG 2 cut(s) 625, 634
BsiHKCI CYCGRG 1 cut(s) 316
BsiSI CCGG 4 cut(s) 201, 211, 625, 725
BslFI GGGAC 1 cut(s) 606
BslI CCNNNNNNNGG 2 cut(s) 32, 836
BsmAI GTCTC 2 cut(s) 764, 774
BsmFI GGGAC 1 cut(s) 606
BsnI GGCC 2 cut(s) 145, 624
Bso31I GGTCTC 2 cut(s) 764, 774
BsoBI CYCGRG 1 cut(s) 316
Bsp1286I GDGCHC 1 cut(s) 220
Bsp13I TCCGGA 1 cut(s) 210
Bsp143I GATC 8 cut(s) 35, 174, 190, 280, 320, 816, 1015, 1027
BspACI CCGC 5 cut(s) 537, 609, 618, 621, 630
BspANI GGCC 2 cut(s) 145, 624
BspCNI CTCAG 3 cut(s) 68, 232, 496
BspEI TCCGGA 1 cut(s) 210
BspFNI CGCG 1 cut(s) 811
BspHI TCATGA 1 cut(s) 1065
BspLI GGNNCC 2 cut(s) 37, 1036
BspOI GCTAGC 1 cut(s) 969
BspPI GGATC 5 cut(s) 30, 43, 275, 315, 811
BspQI GCTCTTC 1 cut(s) 22
BspT107I GGYRCC 1 cut(s) 1034
BspTI CTTAAG 1 cut(s) 901
BspTNI GGTCTC 2 cut(s) 764, 774
BsrDI GCAATG 1 cut(s) 335
BsrI ACTGG 1 cut(s) 26
BssECI CCNNGG 4 cut(s) 140, 324, 625, 861
BssMI GATC 8 cut(s) 35, 174, 190, 280, 320, 816, 1015, 1027
BssNI GRCGYC 1 cut(s) 1035
BssT1I CCWWGG 1 cut(s) 861
Bst2UI CCWGG 2 cut(s) 141, 325
Bst4CI ACNGT 3 cut(s) 164, 351, 855
Bst6I CTCTTC 4 cut(s) 22, 420, 432, 471
BstACI GRCGYC 1 cut(s) 1035
BstAFI CTTAAG 1 cut(s) 901
BstAPI GCANNNNNTGC 2 cut(s) 254, 962
BstC8I GCNNGC 1 cut(s) 967
BstDEI CTNAG 4 cut(s) 8, 55, 219, 504
BstF5I GGATG 1 cut(s) 721
BstFNI CGCG 1 cut(s) 811
BstH2I RGCGCY 1 cut(s) 1038
BstHHI GCGC 1 cut(s) 1037
BstKTI GATC 8 cut(s) 38, 177, 193, 283, 323, 819, 1018, 1030
BstMAI GTCTC 2 cut(s) 764, 774
BstMBI GATC 8 cut(s) 35, 174, 190, 280, 320, 816, 1015, 1027
BstMCI CGRYCG 2 cut(s) 625, 634
BstMWI GCNNNNNNNGC 6 cut(s) 254, 615, 627, 857, 926, 962
BstNI CCWGG 2 cut(s) 141, 325
BstSCI CCNGG 5 cut(s) 139, 200, 323, 624, 724
BstUI CGCG 1 cut(s) 811
BstV1I GCAGC 3 cut(s) 241, 916, 968
BstV2I GAAGAC 1 cut(s) 996
BstX2I RGATCY 3 cut(s) 35, 320, 1027
BstYI RGATCY 3 cut(s) 35, 320, 1027
BstZI CGGCCG 1 cut(s) 622
BsuRI GGCC 2 cut(s) 145, 624
BtsCI GGATG 1 cut(s) 721
BtsI GCAGTG 1 cut(s) 661
BtsIMutI CAGTG 1 cut(s) 661
Cac8I GCNNGC 1 cut(s) 967
CciI TCATGA 1 cut(s) 1065
CfoI GCGC 1 cut(s) 1037
Cfr13I GGNCC 1 cut(s) 143
CseI GACGC 1 cut(s) 246
Csp6I GTAC 2 cut(s) 301, 856
CviAII CATG 5 cut(s) 884, 953, 1039, 1055, 1066
CviQI GTAC 2 cut(s) 301, 856
DdeI CTNAG 4 cut(s) 8, 55, 219, 504
DinI GGCGCC 1 cut(s) 1036
DpnI GATC 8 cut(s) 37, 176, 192, 282, 322, 818, 1017, 1029
DpnII GATC 8 cut(s) 35, 174, 190, 280, 320, 816, 1015, 1027
DrdI GACNNNNNNGTC 2 cut(s) 349, 947
DseDI GACNNNNNNGTC 2 cut(s) 349, 947
EaeI YGGCCR 1 cut(s) 622
EagI CGGCCG 1 cut(s) 622
Eam1104I CTCTTC 4 cut(s) 22, 420, 432, 471
EarI CTCTTC 4 cut(s) 22, 420, 432, 471
EciI GGCGGA 1 cut(s) 624
EclXI CGGCCG 1 cut(s) 622
Eco130I CCWWGG 1 cut(s) 861
Eco24I GRGCYC 1 cut(s) 220
Eco31I GGTCTC 2 cut(s) 764, 774
Eco52I CGGCCG 1 cut(s) 622
Eco88I CYCGRG 1 cut(s) 316
EcoRII CCWGG 2 cut(s) 139, 323
EcoT14I CCWWGG 1 cut(s) 861
EcoT22I ATGCAT 1 cut(s) 582
EcoT38I GRGCYC 1 cut(s) 220
EgeI GGCGCC 1 cut(s) 1036
EheI GGCGCC 1 cut(s) 1036
ErhI CCWWGG 1 cut(s) 861
FaeI CATG 5 cut(s) 887, 956, 1042, 1058, 1069
FalI AAGNNNNNCTT 1 cut(s) 33
FaqI GGGAC 1 cut(s) 606
FatI CATG 5 cut(s) 883, 952, 1038, 1054, 1065
FauI CCCGC 1 cut(s) 530
FauNDI CATATG 2 cut(s) 250, 961
Fnu4HI GCNGC 5 cut(s) 255, 619, 622, 930, 957
FokI GGATG 1 cut(s) 708
FriOI GRGCYC 1 cut(s) 220
Fsp4HI GCNGC 5 cut(s) 255, 619, 622, 930, 957
FspBI CTAG 3 cut(s) 743, 821, 966
GlaI GCGC 1 cut(s) 1036
GluI GCNGC 5 cut(s) 255, 619, 622, 930, 957
HaeII RGCGCY 1 cut(s) 1038
HaeIII GGCC 2 cut(s) 145, 624
HapII CCGG 4 cut(s) 201, 211, 625, 725
HgaI GACGC 1 cut(s) 246
HhaI GCGC 1 cut(s) 1037
Hin1I GRCGYC 1 cut(s) 1035
Hin1II CATG 5 cut(s) 887, 956, 1042, 1058, 1069
Hin6I GCGC 1 cut(s) 1035
HinP1I GCGC 1 cut(s) 1035
HinfI GANTC 2 cut(s) 64, 1062
HpaII CCGG 4 cut(s) 201, 211, 625, 725
HphI GGTGA 7 cut(s) 162, 395, 470, 496, 505, 683, 851
Hpy166II GTNNAC 1 cut(s) 68
Hpy188I TCNGA 2 cut(s) 226, 505
Hpy188III TCNNGA 6 cut(s) 211, 318, 572, 635, 938, 1066
Hpy8I GTNNAC 1 cut(s) 68
HpyAV CCTTC 6 cut(s) 50, 233, 295, 475, 484, 794
HpyCH4III ACNGT 3 cut(s) 164, 351, 855
HpyCH4V TGCA 9 cut(s) 47, 340, 523, 580, 587, 880, 932, 956, 1076
HpyF10VI GCNNNNNNNGC 6 cut(s) 254, 615, 627, 857, 926, 962
HpyF3I CTNAG 4 cut(s) 8, 55, 219, 504
Hsp92I GRCGYC 1 cut(s) 1035
Hsp92II CATG 5 cut(s) 887, 956, 1042, 1058, 1069
HspAI GCGC 1 cut(s) 1035
KasI GGCGCC 1 cut(s) 1034
Kpn2I TCCGGA 1 cut(s) 210
Kzo9I GATC 8 cut(s) 35, 174, 190, 280, 320, 816, 1015, 1027
LguI GCTCTTC 1 cut(s) 22
LmnI GCTCC 1 cut(s) 694
Lsp1109I GCAGC 3 cut(s) 241, 916, 968
LweI GCATC 2 cut(s) 56, 589
MaeI CTAG 3 cut(s) 743, 821, 966
MaeIII GTNAC 1 cut(s) 671
MalI GATC 8 cut(s) 37, 176, 192, 282, 322, 818, 1017, 1029
MboI GATC 8 cut(s) 35, 174, 190, 280, 320, 816, 1015, 1027
MflI RGATCY 3 cut(s) 35, 320, 1027
MhlI GDGCHC 1 cut(s) 220
MluCI AATT 3 cut(s) 748, 757, 843
Mly113I GGCGCC 1 cut(s) 1035
MlyI GAGTC 1 cut(s) 73
Mph1103I ATGCAT 1 cut(s) 582
MroI TCCGGA 1 cut(s) 210
MroXI GAANNNNTTC 1 cut(s) 16
MseI TTAA 1 cut(s) 902
MslI CAYNNNNRTG 1 cut(s) 885
MspCI CTTAAG 1 cut(s) 901
MspI CCGG 4 cut(s) 201, 211, 625, 725
MspR9I CCNGG 5 cut(s) 141, 202, 325, 626, 726
MvaI CCWGG 2 cut(s) 141, 325
MvnI CGCG 1 cut(s) 811
MwoI GCNNNNNNNGC 6 cut(s) 254, 615, 627, 857, 926, 962
NarI GGCGCC 1 cut(s) 1035
NciI CCSGG 3 cut(s) 202, 626, 726
NdeI CATATG 2 cut(s) 250, 961
NdeII GATC 8 cut(s) 35, 174, 190, 280, 320, 816, 1015, 1027
NheI GCTAGC 1 cut(s) 965
NlaIII CATG 5 cut(s) 887, 956, 1042, 1058, 1069
NlaIV GGNNCC 2 cut(s) 37, 1036
NmeAIII GCCGAG 2 cut(s) 85, 350
NmuCI GTSAC 1 cut(s) 671
NsiI ATGCAT 1 cut(s) 582
PaeR7I CTCGAG 1 cut(s) 316
PagI TCATGA 1 cut(s) 1065
PciSI GCTCTTC 1 cut(s) 22
PdmI GAANNNNTTC 1 cut(s) 16
PfeI GAWTC 1 cut(s) 1062
PflMI CCANNNNNTGG 1 cut(s) 32
PfoI TCCNGGA 1 cut(s) 724
PkrI GCNGC 5 cut(s) 256, 620, 623, 931, 958
PleI GAGTC 1 cut(s) 72
PluTI GGCGCC 1 cut(s) 1038
PpsI GAGTC 1 cut(s) 72
Psp6I CCWGG 2 cut(s) 139, 323
PspGI CCWGG 2 cut(s) 139, 323
PspN4I GGNNCC 2 cut(s) 37, 1036
PspPI GGNCC 1 cut(s) 143
PsuI RGATCY 3 cut(s) 35, 320, 1027
RsaI GTAC 2 cut(s) 302, 857
RsaNI GTAC 2 cut(s) 301, 856
RseI CAYNNNNRTG 1 cut(s) 885
SapI GCTCTTC 1 cut(s) 22
SaqAI TTAA 1 cut(s) 902
SatI GCNGC 5 cut(s) 255, 619, 622, 930, 957
Sau3AI GATC 8 cut(s) 35, 174, 190, 280, 320, 816, 1015, 1027
Sau96I GGNCC 1 cut(s) 143
SchI GAGTC 1 cut(s) 73
ScrFI CCNGG 5 cut(s) 141, 202, 325, 626, 726
SduI GDGCHC 1 cut(s) 220
SfaNI GCATC 2 cut(s) 56, 589
SfoI GGCGCC 1 cut(s) 1036
Sfr274I CTCGAG 1 cut(s) 316
SlaI CTCGAG 1 cut(s) 316
SmiMI CAYNNNNRTG 1 cut(s) 885
SmlI CTYRAG 3 cut(s) 316, 901, 936
SmoI CTYRAG 3 cut(s) 316, 901, 936
Sse9I AATT 3 cut(s) 748, 757, 843
SsiI CCGC 5 cut(s) 537, 609, 618, 621, 630
SspDI GGCGCC 1 cut(s) 1034
SspMI CTAG 3 cut(s) 743, 821, 966
StyD4I CCNGG 5 cut(s) 139, 200, 323, 624, 724
StyI CCWWGG 1 cut(s) 861
TaaI ACNGT 3 cut(s) 164, 351, 855
TaqI TCGA 3 cut(s) 317, 359, 815
TasI AATT 3 cut(s) 748, 757, 843
TauI GCSGC 2 cut(s) 621, 624
TfiI GAWTC 1 cut(s) 1062
Tru1I TTAA 1 cut(s) 902
Tru9I TTAA 1 cut(s) 902
TscAI CASTG 1 cut(s) 661
TseFI GTSAC 1 cut(s) 671
TseI GCWGC 3 cut(s) 254, 929, 956
Tsp45I GTSAC 1 cut(s) 671
TspRI CASTG 1 cut(s) 661
Van91I CCANNNNNTGG 1 cut(s) 32
Vha464I CTTAAG 1 cut(s) 901
XapI RAATTY 1 cut(s) 843
XhoI CTCGAG 1 cut(s) 316
XmnI GAANNNNTTC 1 cut(s) 16
XspI CTAG 3 cut(s) 743, 821, 966
Zsp2I ATGCAT 1 cut(s) 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.