pycom05g17370

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0012577
Physical Location & Seq
Forward (+)
20536384 .. 20537445
1062 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g17370.1

Sequence Viewer

Length: 867 bp
ATGTTGCTAAGGAGCTCATCAACCCCAGTGATTGGATCTCTCCTTGCATCATACGCCTCAGAAAGCCCTAACATCATCAGCAATCATCACTCAGATCCCAAACCAAACACCTTCCACCAACATGCTCCCACCAAGTTCTCATTCCACCAACCTGGATATCTCAACATCTCACACAACTCCTCACCAATCTCTCAGTCCTCCCTTGATTTGGACCGGCATTCGCTAACCGGGTTCCGAAGGGCTCAGTCTGATGGGAACTTGGAGGGACTGGCCTATGCTTCTTGTGACACTGAGGATCAGTTCTTTGATTCAAAACTACTCAACATGTTCTCCGCAAGATCCAAGCGCACGATGCTGCAAACTATACCGTCTCCTTCGTTGCATAAGTCGGGTGGTTACGACGACGATGAGGACGATAGTGATGTGGAATACGAGGAAGAAGAAGAAGAAGAAGAGTTTGATGAGGAGAATGTGGAGAGAGTGGCGGCGATGGAACGCAAAAATGTTTGTTTAGAGGAGAAGGTGAAGAACATGAGCTTAACTCGGGATGTGAAGTTTTTGGACAAAATATGGAATATAGGGTTTGAGGAAAAAAGAGAGCTTGCGAGTCAAGAAATGTATCTTGCAAGGGGGCTAGGGTTTGGTGGTGGTAGTGGTGGTGGTGGTGGAAGCGGTAGCCGCGGTGGTGGAGATGTTAATTGGGATGGCTATGATGGTGATGGAGGAGATGACAACCATGGGGCTGAGACGCATCACAAGAAGCTGGTTGAGGAAAATCCAGGCAACCCTTTGTTCCTTAGAAATTATGCTCAATTTTTGTATAAGACCAAGAGAGATCGTTCATGGGGCAGAGGAGTACTACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_19

Protein Analysis

289

Amino Acids

32.08

Weight (kDa)

5.0

Isoelectric Point (pI)

70.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 32
AccII CGCG 1 cut(s) 681
AciI CCGC 5 cut(s) 333, 485, 672, 679, 681
AclWI GGATC 4 cut(s) 43, 89, 303, 333
AfaI GTAC 1 cut(s) 858
AfiI CCNNNNNNNGG 2 cut(s) 32, 208
AflIII ACRYGT 1 cut(s) 324
AgsI TTSAA 1 cut(s) 312
AjnI CCWGG 2 cut(s) 151, 778
AjuI GAANNNNNNNTTGG 2 cut(s) 125, 157
AluBI AGCT 4 cut(s) 15, 537, 601, 763
AluI AGCT 4 cut(s) 15, 537, 601, 763
Alw21I GWGCWC 1 cut(s) 17
Alw26I GTCTC 2 cut(s) 375, 740
AlwI GGATC 4 cut(s) 43, 89, 303, 333
Ama87I CYCGRG 1 cut(s) 543
AoxI GGCC 1 cut(s) 270
ApeKI GCWGC 1 cut(s) 355
AspLEI GCGC 1 cut(s) 348
AspS9I GGNCC 1 cut(s) 211
AsuC2I CCSGG 1 cut(s) 229
AsuHPI GGTGA 3 cut(s) 174, 535, 728
AvaI CYCGRG 1 cut(s) 543
AvaII GGWCC 1 cut(s) 211
BanII GRGCYC 2 cut(s) 17, 244
Bbv12I GWGCWC 1 cut(s) 17
BbvI GCAGC 1 cut(s) 342
BccI CCATC 5 cut(s) 245, 484, 698, 707, 713
BciT130I CCWGG 2 cut(s) 153, 780
BcnI CCSGG 1 cut(s) 229
BcoDI GTCTC 2 cut(s) 375, 740
BfaI CTAG 1 cut(s) 635
BfmI CTRYAG 1 cut(s) 863
BisI GCNGC 3 cut(s) 356, 486, 679
BlsI GCNGC 3 cut(s) 357, 487, 680
BmcAI AGTACT 1 cut(s) 858
Bme1390I CCNGG 3 cut(s) 153, 229, 780
Bme18I GGWCC 1 cut(s) 211
BmeT110I CYCGRG 1 cut(s) 543
BmgT120I GGNCC 1 cut(s) 211
BmiI GGNNCC 1 cut(s) 233
BmrFI CCNGG 3 cut(s) 153, 229, 780
BmrI ACTGGG 1 cut(s) 20
BmsI GCATC 3 cut(s) 56, 342, 760
BmuI ACTGGG 1 cut(s) 20
BplI GAGNNNNNCTC 2 cut(s) 526, 558
Bpu10I CCTNAGC 1 cut(s) 8
BpuMI CCSGG 1 cut(s) 229
BsaJI CCNNGG 2 cut(s) 679, 736
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
Bsc4I CCNNNNNNNGG 2 cut(s) 32, 208
Bse118I RCCGGY 1 cut(s) 213
Bse1I ACTGG 2 cut(s) 26, 273
BseBI CCWGG 2 cut(s) 153, 780
BseDI CCNNGG 2 cut(s) 679, 736
BseGI GGATG 2 cut(s) 553, 709
BseLI CCNNNNNNNGG 2 cut(s) 32, 208
BseMII CTCAG 6 cut(s) 72, 105, 206, 257, 282, 735
BseNI ACTGG 2 cut(s) 26, 273
BseRI GAGGAG 5 cut(s) 169, 479, 530, 738, 867
BseXI GCAGC 1 cut(s) 342
Bsh1236I CGCG 1 cut(s) 681
BshFI GGCC 1 cut(s) 272
BsiHKAI GWGCWC 1 cut(s) 17
BsiHKCI CYCGRG 1 cut(s) 543
BsiSI CCGG 2 cut(s) 214, 228
BslFI GGGAC 1 cut(s) 279
BslI CCNNNNNNNGG 2 cut(s) 32, 208
BsmAI GTCTC 2 cut(s) 375, 740
BsmBI CGTCTC 2 cut(s) 375, 740
BsmFI GGGAC 1 cut(s) 279
BsmI GAATGC 1 cut(s) 217
BsnI GGCC 1 cut(s) 272
BsoBI CYCGRG 1 cut(s) 543
Bsp1286I GDGCHC 2 cut(s) 17, 244
Bsp143I GATC 5 cut(s) 35, 94, 295, 338, 835
Bsp19I CCATGG 1 cut(s) 736
BspACI CCGC 5 cut(s) 333, 485, 672, 679, 681
BspANI GGCC 1 cut(s) 272
BspCNI CTCAG 6 cut(s) 71, 104, 205, 256, 283, 736
BspFNI CGCG 1 cut(s) 681
BspLI GGNNCC 1 cut(s) 233
BspPI GGATC 4 cut(s) 43, 89, 303, 333
BsrFI RCCGGY 1 cut(s) 213
BsrI ACTGG 2 cut(s) 26, 273
BssAI RCCGGY 1 cut(s) 213
BssECI CCNNGG 2 cut(s) 679, 736
BssMI GATC 5 cut(s) 35, 94, 295, 338, 835
BssT1I CCWWGG 1 cut(s) 736
Bst2UI CCWGG 2 cut(s) 153, 780
Bst4CI ACNGT 1 cut(s) 369
Bst6I CTCTTC 1 cut(s) 447
BstC8I GCNNGC 1 cut(s) 603
BstDEI CTNAG 8 cut(s) 8, 58, 91, 192, 243, 291, 744, 797
BstDSI CCRYGG 2 cut(s) 679, 736
BstF5I GGATG 2 cut(s) 553, 709
BstFNI CGCG 1 cut(s) 681
BstHHI GCGC 1 cut(s) 348
BstKTI GATC 5 cut(s) 38, 97, 298, 341, 838
BstMAI GTCTC 2 cut(s) 375, 740
BstMBI GATC 5 cut(s) 35, 94, 295, 338, 835
BstMWI GCNNNNNNNGC 3 cut(s) 53, 352, 678
BstNI CCWGG 2 cut(s) 153, 780
BstNSI RCATGY 2 cut(s) 125, 328
BstSCI CCNGG 3 cut(s) 151, 227, 778
BstSFI CTRYAG 1 cut(s) 863
BstUI CGCG 1 cut(s) 681
BstV1I GCAGC 1 cut(s) 342
BstX2I RGATCY 3 cut(s) 35, 94, 338
BstXI CCANNNNNNTGG 1 cut(s) 152
BstYI RGATCY 3 cut(s) 35, 94, 338
BsuRI GGCC 1 cut(s) 272
BtgI CCRYGG 2 cut(s) 679, 736
BtgZI GCGATG 1 cut(s) 503
BtsCI GGATG 2 cut(s) 553, 709
BtsIMutI CAGTG 2 cut(s) 33, 288
Cac8I GCNNGC 1 cut(s) 603
CfoI GCGC 1 cut(s) 348
Cfr10I RCCGGY 1 cut(s) 213
Cfr13I GGNCC 1 cut(s) 211
Cfr42I CCGCGG 1 cut(s) 682
CseI GACGC 1 cut(s) 757
Csp6I GTAC 1 cut(s) 857
CviAII CATG 5 cut(s) 122, 325, 532, 737, 843
CviQI GTAC 1 cut(s) 857
DdeI CTNAG 8 cut(s) 8, 58, 91, 192, 243, 291, 744, 797
DpnI GATC 5 cut(s) 37, 96, 297, 340, 837
DpnII GATC 5 cut(s) 35, 94, 295, 338, 835
Eam1104I CTCTTC 1 cut(s) 447
EarI CTCTTC 1 cut(s) 447
Ecl136II GAGCTC 1 cut(s) 15
Eco130I CCWWGG 1 cut(s) 736
Eco24I GRGCYC 2 cut(s) 17, 244
Eco32I GATATC 1 cut(s) 158
Eco47I GGWCC 1 cut(s) 211
Eco53kI GAGCTC 1 cut(s) 15
Eco88I CYCGRG 1 cut(s) 543
EcoICRI GAGCTC 1 cut(s) 15
EcoRII CCWGG 2 cut(s) 151, 778
EcoRV GATATC 1 cut(s) 158
EcoT14I CCWWGG 1 cut(s) 736
EcoT38I GRGCYC 2 cut(s) 17, 244
ErhI CCWWGG 1 cut(s) 736
Esp3I CGTCTC 2 cut(s) 375, 740
FaeI CATG 5 cut(s) 125, 328, 535, 740, 846
FaqI GGGAC 1 cut(s) 279
FatI CATG 5 cut(s) 121, 324, 531, 736, 842
Fnu4HI GCNGC 3 cut(s) 356, 486, 679
FokI GGATG 2 cut(s) 560, 716
FriOI GRGCYC 2 cut(s) 17, 244
Fsp4HI GCNGC 3 cut(s) 356, 486, 679
FspBI CTAG 1 cut(s) 635
GlaI GCGC 1 cut(s) 347
GluI GCNGC 3 cut(s) 356, 486, 679
HaeIII GGCC 1 cut(s) 272
HapII CCGG 2 cut(s) 214, 228
HgaI GACGC 1 cut(s) 757
HhaI GCGC 1 cut(s) 348
Hin1II CATG 5 cut(s) 125, 328, 535, 740, 846
Hin6I GCGC 1 cut(s) 346
HinP1I GCGC 1 cut(s) 346
HinfI GANTC 2 cut(s) 308, 607
HpaII CCGG 2 cut(s) 214, 228
HphI GGTGA 3 cut(s) 174, 535, 728
Hpy188I TCNGA 4 cut(s) 61, 94, 236, 250
Hpy188III TCNNGA 2 cut(s) 545, 611
Hpy99I CGWCG 2 cut(s) 404, 407
HpyAV CCTTC 4 cut(s) 121, 231, 384, 514
HpyCH4III ACNGT 1 cut(s) 369
HpyCH4V TGCA 4 cut(s) 47, 358, 382, 626
HpyF10VI GCNNNNNNNGC 3 cut(s) 53, 352, 678
HpyF3I CTNAG 8 cut(s) 8, 58, 91, 192, 243, 291, 744, 797
Hsp92II CATG 5 cut(s) 125, 328, 535, 740, 846
HspAI GCGC 1 cut(s) 346
KspI CCGCGG 1 cut(s) 682
Kzo9I GATC 5 cut(s) 35, 94, 295, 338, 835
LmnI GCTCC 2 cut(s) 12, 130
LpnPI CCDG 9 cut(s) 39, 138, 165, 227, 241, 254, 749, 765, 792
Lsp1109I GCAGC 1 cut(s) 342
LweI GCATC 3 cut(s) 56, 342, 760
MaeI CTAG 1 cut(s) 635
MaeIII GTNAC 2 cut(s) 284, 395
MalI GATC 5 cut(s) 37, 96, 297, 340, 837
MboI GATC 5 cut(s) 35, 94, 295, 338, 835
MboII GAAGA 7 cut(s) 449, 452, 455, 458, 461, 464, 538
MflI RGATCY 3 cut(s) 35, 94, 338
MhlI GDGCHC 2 cut(s) 17, 244
MluCI AATT 3 cut(s) 697, 802, 812
MlyI GAGTC 1 cut(s) 616
MseI TTAA 2 cut(s) 539, 696
MslI CAYNNNNRTG 1 cut(s) 120
MspA1I CMGCKG 1 cut(s) 681
MspI CCGG 2 cut(s) 214, 228
MspR9I CCNGG 3 cut(s) 153, 229, 780
Mva1269I GAATGC 1 cut(s) 217
MvaI CCWGG 2 cut(s) 153, 780
MvnI CGCG 1 cut(s) 681
MwoI GCNNNNNNNGC 3 cut(s) 53, 352, 678
NciI CCSGG 1 cut(s) 229
NcoI CCATGG 1 cut(s) 736
NdeII GATC 5 cut(s) 35, 94, 295, 338, 835
NlaIII CATG 5 cut(s) 125, 328, 535, 740, 846
NlaIV GGNNCC 1 cut(s) 233
NmuCI GTSAC 1 cut(s) 284
NspI RCATGY 2 cut(s) 125, 328
PciI ACATGT 1 cut(s) 324
PcsI WCGNNNNNNNCGW 1 cut(s) 411
PctI GAATGC 1 cut(s) 217
PfeI GAWTC 1 cut(s) 308
PflMI CCANNNNNTGG 1 cut(s) 32
PkrI GCNGC 3 cut(s) 357, 487, 680
PleI GAGTC 1 cut(s) 615
PpsI GAGTC 1 cut(s) 615
PscI ACATGT 1 cut(s) 324
Psp124BI GAGCTC 1 cut(s) 17
Psp6I CCWGG 2 cut(s) 151, 778
PspGI CCWGG 2 cut(s) 151, 778
PspN4I GGNNCC 1 cut(s) 233
PspPI GGNCC 1 cut(s) 211
PsuI RGATCY 3 cut(s) 35, 94, 338
RsaI GTAC 1 cut(s) 858
RsaNI GTAC 1 cut(s) 857
RseI CAYNNNNRTG 1 cut(s) 120
SacI GAGCTC 1 cut(s) 17
SacII CCGCGG 1 cut(s) 682
SaqAI TTAA 2 cut(s) 539, 696
SatI GCNGC 3 cut(s) 356, 486, 679
Sau3AI GATC 5 cut(s) 35, 94, 295, 338, 835
Sau96I GGNCC 1 cut(s) 211
ScaI AGTACT 1 cut(s) 858
SchI GAGTC 1 cut(s) 616
ScrFI CCNGG 3 cut(s) 153, 229, 780
SduI GDGCHC 2 cut(s) 17, 244
SetI ASST 7 cut(s) 17, 113, 154, 525, 539, 603, 765
SfaNI GCATC 3 cut(s) 56, 342, 760
SfcI CTRYAG 1 cut(s) 863
Sfr303I CCGCGG 1 cut(s) 682
SgrBI CCGCGG 1 cut(s) 682
SinI GGWCC 1 cut(s) 211
SmiMI CAYNNNNRTG 1 cut(s) 120
Sse9I AATT 3 cut(s) 697, 802, 812
SsiI CCGC 5 cut(s) 333, 485, 672, 679, 681
SspMI CTAG 1 cut(s) 635
SstI GAGCTC 1 cut(s) 17
StyD4I CCNGG 3 cut(s) 151, 227, 778
StyI CCWWGG 1 cut(s) 736
TaaI ACNGT 1 cut(s) 369
TasI AATT 3 cut(s) 697, 802, 812
TatI WGTACW 1 cut(s) 856
TauI GCSGC 2 cut(s) 488, 681
TfiI GAWTC 1 cut(s) 308
Tru1I TTAA 2 cut(s) 539, 696
Tru9I TTAA 2 cut(s) 539, 696
TscAI CASTG 2 cut(s) 33, 295
TseFI GTSAC 1 cut(s) 284
TseI GCWGC 1 cut(s) 355
Tsp45I GTSAC 1 cut(s) 284
TspDTI ATGAA 1 cut(s) 831
TspRI CASTG 2 cut(s) 33, 295
Van91I CCANNNNNTGG 1 cut(s) 32
VpaK11BI GGWCC 1 cut(s) 211
XceI RCATGY 2 cut(s) 125, 328
XspI CTAG 1 cut(s) 635
ZrmI AGTACT 1 cut(s) 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.