FvH4_2g23890

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0016518
Physical Location & Seq
Reverse (-)
19530033 .. 19531118
1086 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g23890.t1

Sequence Viewer

Length: 1086 bp
ATGGGATTTAAACACAGAAAGTTGTTTGAGGAAAGTCAGGAAACACTCTGCATATGCAAGGGTACTACTATACCTTATCGCTGCCCATGTAAACATCCTAAATCCAAAACCTTCATAGTGGTCGGATCGGCGTTGGCTGCAATCGCTTTGCTTGTTATTTGTGGCTATTTTCTCTATGTAAAGTACTACAAAAACAGAAGATCATCACAACCTCAACCACAACCACAACCTCAACAGGAGGAGGAGACTCTTCATCGTCATGATAATCAGGCTGAGTTGTTTATCGATGAAGAGCATGGACCTGTGGTTGATCACCCCATATGGTACATCCGCACCGTCGGCCTGGAACAATCGCTTATCAGCTCCATCAGCGTGTGCCAGTACAAGAAAGGCGATGGCCTTGTTGAAGGTACAGAGTGCGCTGTGTGCTTGAGTGAGTTCCAAGAAGATGAGACTTTGAGACTTTTGCCCAAGTGTGATCATGCTTTTCATATTCCCTGTATCGATACTTGGCTTACATCTCATACCAATTGCCCCTTGTGCCGAGCTCCCATTATCAAAACCGGCGCAACAGATGTGGCACCACCGCAGCCAAATGTTGCAGCTGACTCAAGTGAGCCTGCAGAGGAAGAAACCCCGGTTGAGGTTCTAGAAAATACAGGTGAGTCTGGTACTAGTACTGGAGGAGTTGAAGATGAATTGTGTGATGGGATTATTAGGACTACTGAAGTAGGGGAAGATGATATCGTTAACAAACAAATCTGTGTCAACGAGGAGGAGGTGGATTATGGTGTGGAGGAGGCAAGAAGAAGATCGGTTTCCATGGATTCTGCATCAGCTTTGAAGATTAGTCTTGCGGTTTCTAATGTTGTTGCAGTGGATAAATCAAACAGGGGGGTTGCAAGTGTTGCCAAGAGGGTTGGGGGAGATCAGAAATTGTCAAGACAGAAAGGTAGTTCCTCAAGAGGGGCATCTTTACCAAAGGGAAAACAAACAATTCAGAAGTCGTCTTCCTGGAATGGCAAGTTCTCATTATGCATGGACATCCACAACTGTGACTCCGTTGTTCCACTGCGAAGCTTTTGA

Protein Analysis

362

Amino Acids

39.69

Weight (kDa)

5.47

Isoelectric Point (pI)

53.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 139 - 182 2.4e-13 Ring finger domain
zf-RING_11 PF17123 139 - 168 4.4e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 139 - 181 1.8e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 140 - 182 2.6e-08 RING-H2 zinc finger domain
zf-C3HC4 PF00097 140 - 181 5.4e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016518)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33565
fragaria_vesca FvH4_2g23890
prunus_persica Prupe.8G066400_v2.0.a1 Prupe.8G066400_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0291501
rosa_laevigata RLG00000012111
rosa_multiflora Rmu_sc0024207.1_g000003 Rmu_sc0026626.1_g000002
rosa_rugosa Rorug06G0220000
rosa_samantha Rh6AG332100 Rh6BG339000 Rh6CG345200 Rh6DG332600
rosa_wichuraiana Rw6G028850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 580
AciI CCGC 3 cut(s) 331, 587, 857
AclWI GGATC 1 cut(s) 133
AcuI CTGAAG 1 cut(s) 747
AfaI GTAC 7 cut(s) 64, 185, 326, 383, 412, 673, 679
AfiI CCNNNNNNNGG 2 cut(s) 643, 966
AgsI TTSAA 3 cut(s) 407, 692, 844
AhlI ACTAGT 1 cut(s) 674
AjnI CCWGG 2 cut(s) 342, 1013
AleI CACNNNNGTG 1 cut(s) 1053
AluBI AGCT 5 cut(s) 363, 548, 605, 839, 1080
AluI AGCT 5 cut(s) 363, 548, 605, 839, 1080
Alw21I GWGCWC 1 cut(s) 550
Alw26I GTCTC 3 cut(s) 239, 446, 454
AlwI GGATC 1 cut(s) 133
AoxI GGCC 2 cut(s) 340, 397
ApeKI GCWGC 4 cut(s) 81, 137, 589, 602
AspLEI GCGC 2 cut(s) 422, 569
AspS9I GGNCC 1 cut(s) 299
AsuC2I CCSGG 1 cut(s) 638
AsuHPI GGTGA 2 cut(s) 305, 674
AvaII GGWCC 1 cut(s) 299
BanI GGYRCC 1 cut(s) 580
BanII GRGCYC 1 cut(s) 550
BbsI GAAGAC 1 cut(s) 1002
Bbv12I GWGCWC 1 cut(s) 550
BbvI GCAGC 4 cut(s) 68, 124, 601, 614
BccI CCATC 3 cut(s) 374, 389, 701
BciT130I CCWGG 2 cut(s) 344, 1015
BclI TGATCA 2 cut(s) 310, 478
BcnI CCSGG 1 cut(s) 638
BcoDI GTCTC 3 cut(s) 239, 446, 454
BcuI ACTAGT 1 cut(s) 674
BfaI CTAG 2 cut(s) 650, 675
BfmI CTRYAG 1 cut(s) 621
BisI GCNGC 4 cut(s) 82, 138, 590, 603
BlsI GCNGC 4 cut(s) 83, 139, 591, 604
BmcAI AGTACT 2 cut(s) 185, 679
Bme1390I CCNGG 3 cut(s) 344, 638, 1015
Bme18I GGWCC 1 cut(s) 299
BmgT120I GGNCC 1 cut(s) 299
BmiI GGNNCC 1 cut(s) 582
BmrFI CCNGG 3 cut(s) 344, 638, 1015
BmsI GCATC 2 cut(s) 842, 980
BpiI GAAGAC 1 cut(s) 1002
BpmI CTGGAG 1 cut(s) 702
BpuEI CTTGAG 3 cut(s) 451, 595, 946
BpuMI CCSGG 1 cut(s) 638
Bsa29I ATCGAT 2 cut(s) 285, 504
BsaJI CCNNGG 2 cut(s) 636, 822
BsaXI ACNNNNNCTCC 2 cut(s) 1043, 1073
Bsc4I CCNNNNNNNGG 2 cut(s) 643, 966
Bse118I RCCGGY 1 cut(s) 563
Bse1I ACTGG 2 cut(s) 379, 685
BseBI CCWGG 2 cut(s) 344, 1015
BseCI ATCGAT 2 cut(s) 285, 504
BseDI CCNNGG 2 cut(s) 636, 822
BseGI GGATG 3 cut(s) 94, 327, 1044
BseLI CCNNNNNNNGG 2 cut(s) 643, 966
BseMII CTCAG 1 cut(s) 264
BseNI ACTGG 2 cut(s) 379, 685
BseRI GAGGAG 6 cut(s) 254, 257, 699, 788, 791, 812
BseXI GCAGC 4 cut(s) 68, 124, 601, 614
BshFI GGCC 2 cut(s) 342, 399
BshNI GGYRCC 1 cut(s) 580
BshVI ATCGAT 2 cut(s) 285, 504
BsiHKAI GWGCWC 1 cut(s) 550
BsiSI CCGG 2 cut(s) 564, 638
BslI CCNNNNNNNGG 2 cut(s) 643, 966
BsmAI GTCTC 3 cut(s) 239, 446, 454
BsnI GGCC 2 cut(s) 342, 399
Bsp1286I GDGCHC 1 cut(s) 550
Bsp143I GATC 6 cut(s) 125, 200, 310, 478, 812, 928
Bsp19I CCATGG 1 cut(s) 822
BspACI CCGC 3 cut(s) 331, 587, 857
BspANI GGCC 2 cut(s) 342, 399
BspCNI CTCAG 1 cut(s) 265
BspDI ATCGAT 2 cut(s) 285, 504
BspHI TCATGA 1 cut(s) 259
BspLI GGNNCC 1 cut(s) 582
BspMAI CTGCAG 1 cut(s) 625
BspPI GGATC 1 cut(s) 133
BspQI GCTCTTC 1 cut(s) 285
BspT107I GGYRCC 1 cut(s) 580
BsrFI RCCGGY 1 cut(s) 563
BsrI ACTGG 2 cut(s) 379, 685
BssAI RCCGGY 1 cut(s) 563
BssECI CCNNGG 2 cut(s) 636, 822
BssMI GATC 6 cut(s) 125, 200, 310, 478, 812, 928
BssT1I CCWWGG 1 cut(s) 822
Bst2UI CCWGG 2 cut(s) 344, 1015
Bst4CI ACNGT 2 cut(s) 337, 1055
Bst6I CTCTTC 2 cut(s) 255, 285
BstAPI GCANNNNNTGC 1 cut(s) 908
BstC8I GCNNGC 1 cut(s) 621
BstDEI CTNAG 1 cut(s) 273
BstDSI CCRYGG 1 cut(s) 822
BstF5I GGATG 3 cut(s) 94, 327, 1044
BstHHI GCGC 2 cut(s) 422, 569
BstKTI GATC 6 cut(s) 128, 203, 313, 481, 815, 931
BstMAI GTCTC 3 cut(s) 239, 446, 454
BstMBI GATC 6 cut(s) 125, 200, 310, 478, 812, 928
BstMWI GCNNNNNNNGC 7 cut(s) 137, 143, 339, 369, 426, 540, 908
BstNI CCWGG 2 cut(s) 344, 1015
BstSCI CCNGG 3 cut(s) 342, 636, 1013
BstSFI CTRYAG 1 cut(s) 621
BstV1I GCAGC 4 cut(s) 68, 124, 601, 614
BstV2I GAAGAC 1 cut(s) 1002
Bsu15I ATCGAT 2 cut(s) 285, 504
BsuRI GGCC 2 cut(s) 342, 399
BsuTUI ATCGAT 2 cut(s) 285, 504
BtgI CCRYGG 1 cut(s) 822
BtgZI GCGATG 1 cut(s) 408
BtsCI GGATG 3 cut(s) 94, 327, 1044
BtsI GCAGTG 2 cut(s) 882, 1070
BtsIMutI CAGTG 2 cut(s) 882, 1070
Cac8I GCNNGC 1 cut(s) 621
CciI TCATGA 1 cut(s) 259
CfoI GCGC 2 cut(s) 422, 569
Cfr10I RCCGGY 1 cut(s) 563
Cfr13I GGNCC 1 cut(s) 299
ClaI ATCGAT 2 cut(s) 285, 504
Csp6I GTAC 7 cut(s) 63, 184, 325, 382, 411, 672, 678
CspCI CAANNNNNGTGG 2 cut(s) 558, 593
CviAII CATG 6 cut(s) 87, 260, 296, 482, 823, 1039
CviQI GTAC 7 cut(s) 63, 184, 325, 382, 411, 672, 678
DdeI CTNAG 1 cut(s) 273
DpnI GATC 6 cut(s) 127, 202, 312, 480, 814, 930
DpnII GATC 6 cut(s) 125, 200, 310, 478, 812, 928
DraI TTTAAA 1 cut(s) 10
Eam1104I CTCTTC 2 cut(s) 255, 285
EarI CTCTTC 2 cut(s) 255, 285
Ecl136II GAGCTC 1 cut(s) 548
Eco130I CCWWGG 1 cut(s) 822
Eco24I GRGCYC 1 cut(s) 550
Eco32I GATATC 1 cut(s) 745
Eco47I GGWCC 1 cut(s) 299
Eco53kI GAGCTC 1 cut(s) 548
Eco57I CTGAAG 1 cut(s) 747
EcoICRI GAGCTC 1 cut(s) 548
EcoRII CCWGG 2 cut(s) 342, 1013
EcoRV GATATC 1 cut(s) 745
EcoT14I CCWWGG 1 cut(s) 822
EcoT22I ATGCAT 1 cut(s) 1040
EcoT38I GRGCYC 1 cut(s) 550
ErhI CCWWGG 1 cut(s) 822
FaeI CATG 6 cut(s) 90, 263, 299, 485, 826, 1042
FatI CATG 6 cut(s) 86, 259, 295, 481, 822, 1038
FauNDI CATATG 2 cut(s) 53, 320
FbaI TGATCA 2 cut(s) 310, 478
Fnu4HI GCNGC 4 cut(s) 82, 138, 590, 603
FokI GGATG 3 cut(s) 81, 314, 1031
FriOI GRGCYC 1 cut(s) 550
Fsp4HI GCNGC 4 cut(s) 82, 138, 590, 603
FspBI CTAG 2 cut(s) 650, 675
GlaI GCGC 2 cut(s) 421, 568
GluI GCNGC 4 cut(s) 82, 138, 590, 603
GsuI CTGGAG 1 cut(s) 702
HaeIII GGCC 2 cut(s) 342, 399
HapII CCGG 2 cut(s) 564, 638
HhaI GCGC 2 cut(s) 422, 569
Hin1II CATG 6 cut(s) 90, 263, 299, 485, 826, 1042
Hin6I GCGC 2 cut(s) 420, 567
HinP1I GCGC 2 cut(s) 420, 567
HincII GTYRAC 2 cut(s) 751, 769
HindII GTYRAC 2 cut(s) 751, 769
HindIII AAGCTT 1 cut(s) 1078
HinfI GANTC 5 cut(s) 247, 608, 665, 827, 1058
HpaI GTTAAC 1 cut(s) 751
HpaII CCGG 2 cut(s) 564, 638
HphI GGTGA 2 cut(s) 305, 674
Hpy166II GTNNAC 3 cut(s) 92, 751, 769
Hpy188I TCNGA 3 cut(s) 125, 933, 1002
Hpy188III TCNNGA 5 cut(s) 38, 260, 650, 942, 963
Hpy8I GTNNAC 3 cut(s) 92, 751, 769
Hpy99I CGWCG 1 cut(s) 341
HpyAV CCTTC 2 cut(s) 121, 401
HpyCH4III ACNGT 2 cut(s) 337, 1055
HpyCH4V TGCA 9 cut(s) 51, 57, 140, 602, 623, 833, 875, 902, 1038
HpyF10VI GCNNNNNNNGC 7 cut(s) 137, 143, 339, 369, 426, 540, 908
HpyF3I CTNAG 1 cut(s) 273
Hsp92II CATG 6 cut(s) 90, 263, 299, 485, 826, 1042
HspAI GCGC 2 cut(s) 420, 567
Ksp22I TGATCA 2 cut(s) 310, 478
KspAI GTTAAC 1 cut(s) 751
Kzo9I GATC 6 cut(s) 125, 200, 310, 478, 812, 928
LguI GCTCTTC 1 cut(s) 285
LmnI GCTCC 2 cut(s) 368, 553
Lsp1109I GCAGC 4 cut(s) 68, 124, 601, 614
LweI GCATC 2 cut(s) 842, 980
MaeI CTAG 2 cut(s) 650, 675
MaeIII GTNAC 1 cut(s) 1055
MalI GATC 6 cut(s) 127, 202, 312, 480, 814, 930
MboI GATC 6 cut(s) 125, 200, 310, 478, 812, 928
MfeI CAATTG 1 cut(s) 529
MhlI GDGCHC 1 cut(s) 550
MluCI AATT 4 cut(s) 529, 698, 935, 996
MlyI GAGTC 4 cut(s) 241, 602, 674, 1052
MmeI TCCRAC 1 cut(s) 103
Mph1103I ATGCAT 1 cut(s) 1040
MseI TTAA 2 cut(s) 9, 750
MslI CAYNNNNRTG 3 cut(s) 258, 371, 1053
MspA1I CMGCKG 1 cut(s) 605
MspI CCGG 2 cut(s) 564, 638
MspR9I CCNGG 3 cut(s) 344, 638, 1015
MunI CAATTG 1 cut(s) 529
MvaI CCWGG 2 cut(s) 344, 1015
MwoI GCNNNNNNNGC 7 cut(s) 137, 143, 339, 369, 426, 540, 908
NciI CCSGG 1 cut(s) 638
NcoI CCATGG 1 cut(s) 822
NdeI CATATG 2 cut(s) 53, 320
NdeII GATC 6 cut(s) 125, 200, 310, 478, 812, 928
NlaIII CATG 6 cut(s) 90, 263, 299, 485, 826, 1042
NlaIV GGNNCC 1 cut(s) 582
NmeAIII GCCGAG 1 cut(s) 569
NmuCI GTSAC 1 cut(s) 1055
NsiI ATGCAT 1 cut(s) 1040
OliI CACNNNNGTG 1 cut(s) 1053
PagI TCATGA 1 cut(s) 259
PciSI GCTCTTC 1 cut(s) 285
PfeI GAWTC 1 cut(s) 827
PfoI TCCNGGA 1 cut(s) 1013
PkrI GCNGC 4 cut(s) 83, 139, 591, 604
PleI GAGTC 4 cut(s) 241, 602, 673, 1052
PpsI GAGTC 4 cut(s) 241, 602, 673, 1052
Psp124BI GAGCTC 1 cut(s) 550
Psp6I CCWGG 2 cut(s) 342, 1013
PspGI CCWGG 2 cut(s) 342, 1013
PspN4I GGNNCC 1 cut(s) 582
PspPI GGNCC 1 cut(s) 299
PstI CTGCAG 1 cut(s) 625
PvuII CAGCTG 1 cut(s) 605
RsaI GTAC 7 cut(s) 64, 185, 326, 383, 412, 673, 679
RsaNI GTAC 7 cut(s) 63, 184, 325, 382, 411, 672, 678
RseI CAYNNNNRTG 3 cut(s) 258, 371, 1053
SacI GAGCTC 1 cut(s) 550
SapI GCTCTTC 1 cut(s) 285
SaqAI TTAA 2 cut(s) 9, 750
SatI GCNGC 4 cut(s) 82, 138, 590, 603
Sau3AI GATC 6 cut(s) 125, 200, 310, 478, 812, 928
Sau96I GGNCC 1 cut(s) 299
ScaI AGTACT 2 cut(s) 185, 679
SchI GAGTC 4 cut(s) 241, 602, 674, 1052
ScrFI CCNGG 3 cut(s) 344, 638, 1015
SduI GDGCHC 1 cut(s) 550
SfaNI GCATC 2 cut(s) 842, 980
SfcI CTRYAG 1 cut(s) 621
SinI GGWCC 1 cut(s) 299
SmiMI CAYNNNNRTG 3 cut(s) 258, 371, 1053
SmlI CTYRAG 3 cut(s) 430, 610, 961
SmoI CTYRAG 3 cut(s) 430, 610, 961
SpeI ACTAGT 1 cut(s) 674
Sse9I AATT 4 cut(s) 529, 698, 935, 996
SsiI CCGC 3 cut(s) 331, 587, 857
SspMI CTAG 2 cut(s) 650, 675
SstI GAGCTC 1 cut(s) 550
StyD4I CCNGG 3 cut(s) 342, 636, 1013
StyI CCWWGG 1 cut(s) 822
TaaI ACNGT 2 cut(s) 337, 1055
TaqI TCGA 2 cut(s) 285, 504
TasI AATT 4 cut(s) 529, 698, 935, 996
TatI WGTACW 3 cut(s) 183, 381, 677
TfiI GAWTC 1 cut(s) 827
Tru1I TTAA 2 cut(s) 9, 750
Tru9I TTAA 2 cut(s) 9, 750
TscAI CASTG 2 cut(s) 882, 1077
TseFI GTSAC 1 cut(s) 1055
TseI GCWGC 4 cut(s) 81, 137, 589, 602
Tsp45I GTSAC 1 cut(s) 1055
TspDTI ATGAA 5 cut(s) 103, 242, 303, 479, 711
TspGWI ACGGA 1 cut(s) 1051
TspRI CASTG 2 cut(s) 882, 1077
VpaK11BI GGWCC 1 cut(s) 299
XbaI TCTAGA 1 cut(s) 649
XspI CTAG 2 cut(s) 650, 675
ZrmI AGTACT 2 cut(s) 185, 679
Zsp2I ATGCAT 1 cut(s) 1040
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.