Rh6DG332600

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0016518
Physical Location & Seq
Reverse (-)
53766543 .. 53769174
2632 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG332600.1

Sequence Viewer

Length: 1107 bp
ATGGGATTAAAACACAGGAAGCTGATGAGCGACGTCTGCAGAACCTGCAAGGTTATAGGTAAGTCTGACTGTTTAGAAGAATGTAAACCCCCACCCCCACCCCCACCCCCCAATAATTCCACTCCCTTCATACCCGTCGCATCGTCGTTGGCTGCAATCGCTTTGCTTGTTATTTGCGGCTATTTTCTCTATGTAAAGTACTACAAAAACAGAAGATCATCATCACAACCACAACAACTACAACATCTACCTCAGCAGGAGGAGGAGACTCTTCATCATCATGATCATCATGCTGATTTGTTTATTGATGAAGAGCATGGACCTGTGGTTGACCACCCCATATGGTACATCCGCACCGTCGGTCTGCAACAATCGCTTATCAGCTCCATCAGCGTGTGCCAGTACAAGAAAGGTGATGGCCTTATCGAAGGGACAGAGTGCGCTGTGTGCTTGAGTGAGTTCCAAGAAGATGAGACTTTGAGACTTCTGCCAAAGTGTGATCATGCTTTTCATATTCCCTGTATTGATACTTGGCTCACATCTCATACAAATTGCCCCTTGTGCCGTGCCCCCATTATCAAAACCGGCGCAACAGATGTGGCACCACCCGAGCCATTTGTTGCAGCTGATTCAAGTGAGCCAGCAGAGGAAGAAACCCCGGTCGAGGTTCTAGAAAATACAGGTGAGTCCAGTACTAGTACTGGAGGAGTTGAAGATGAAATTTGTGATGGGATTAGTAGGACTACTGAAGCAGGGGAAGATGATATCATTAACAAACAGATCTGTATTAATGAGGAGGAGGTGGATGATGATGTGGAGGAGCCAAGAAGAAGATCGGTTTCCATGGATTCTGCATCAGCTTTGAAGATTAGTCTTGCGGTTTCTAATGTTGTTGAGGTGGATAAATCAAACCGGGGGATTGAAATTGTTGCCAAGAGAGTTGGGGGAGATCAGAAATTGTCAAGGCAGAAAGGTAGTTCTTCAAGAGGGGCATGTTTACAAAATGGAAAACAAACAATTCAGAAGTCGTCTTCCTGGAATGGCAAGTTCTCATTATGCAGGGACATCAACAACTGTGACTCCGTTGTTCCACTGCGAAGCTTTTGA

Protein Analysis

368

Amino Acids

40.52

Weight (kDa)

5.11

Isoelectric Point (pI)

65.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 146 - 189 2.4e-13 Ring finger domain
zf-RING_11 PF17123 146 - 175 4.5e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 146 - 188 1.9e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 147 - 189 2.2e-08 RING-H2 zinc finger domain
zf-C3HC4 PF00097 147 - 188 5.5e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016518)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33565
fragaria_vesca FvH4_2g23890
prunus_persica Prupe.8G066400_v2.0.a1 Prupe.8G066400_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0291501
rosa_laevigata RLG00000012111
rosa_multiflora Rmu_sc0024207.1_g000003 Rmu_sc0026626.1_g000002
rosa_rugosa Rorug06G0220000
rosa_samantha Rh6AG332100 Rh6BG339000 Rh6CG345200 Rh6DG332600
rosa_wichuraiana Rw6G028850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 36
Acc36I ACCTGC 1 cut(s) 53
AccB1I GGYRCC 1 cut(s) 601
AciI CCGC 3 cut(s) 177, 352, 878
AcsI RAATTY 1 cut(s) 720
AcuI CTGAAG 1 cut(s) 768
AcyI GRCGYC 1 cut(s) 33
AfaI GTAC 5 cut(s) 200, 347, 404, 694, 700
AfiI CCNNNNNNNGG 1 cut(s) 664
AgsI TTSAA 5 cut(s) 633, 713, 865, 923, 984
AhlI ACTAGT 1 cut(s) 695
AjnI CCWGG 1 cut(s) 1034
AluBI AGCT 5 cut(s) 22, 384, 626, 860, 1101
AluI AGCT 5 cut(s) 22, 384, 626, 860, 1101
Alw26I GTCTC 3 cut(s) 260, 467, 475
AlwNI CAGNNNCTG 1 cut(s) 45
Ama87I CYCGRG 1 cut(s) 608
AoxI GGCC 1 cut(s) 418
ApeKI GCWGC 2 cut(s) 152, 623
ApoI RAATTY 1 cut(s) 720
AseI ATTAAT 1 cut(s) 789
AspLEI GCGC 2 cut(s) 443, 590
AspS9I GGNCC 1 cut(s) 320
AsuC2I CCSGG 2 cut(s) 659, 914
AsuHPI GGTGA 2 cut(s) 425, 695
AvaI CYCGRG 1 cut(s) 608
AvaII GGWCC 1 cut(s) 320
BaeGI GKGCMC 1 cut(s) 571
BanI GGYRCC 1 cut(s) 601
BbsI GAAGAC 1 cut(s) 1023
BbvCI CCTCAGC 1 cut(s) 252
BbvI GCAGC 2 cut(s) 139, 635
BccI CCATC 3 cut(s) 395, 410, 722
BceAI ACGGC 1 cut(s) 549
BciT130I CCWGG 1 cut(s) 1036
BclI TGATCA 2 cut(s) 283, 499
BcnI CCSGG 2 cut(s) 659, 914
BcoDI GTCTC 3 cut(s) 260, 467, 475
BcuI ACTAGT 1 cut(s) 695
BfaI CTAG 2 cut(s) 671, 696
BfmI CTRYAG 1 cut(s) 37
BfuAI ACCTGC 1 cut(s) 53
BglII AGATCT 1 cut(s) 780
BisI GCNGC 3 cut(s) 153, 178, 624
BlsI GCNGC 3 cut(s) 154, 179, 625
BmcAI AGTACT 3 cut(s) 200, 694, 700
Bme1390I CCNGG 3 cut(s) 659, 914, 1036
Bme18I GGWCC 1 cut(s) 320
BmeT110I CYCGRG 1 cut(s) 608
BmgT120I GGNCC 1 cut(s) 320
BmiI GGNNCC 2 cut(s) 603, 822
BmrFI CCNGG 3 cut(s) 659, 914, 1036
BmsI GCATC 2 cut(s) 149, 863
BpiI GAAGAC 1 cut(s) 1023
BpmI CTGGAG 1 cut(s) 723
Bpu10I CCTNAGC 1 cut(s) 252
BpuEI CTTGAG 1 cut(s) 472
BpuMI CCSGG 2 cut(s) 659, 914
BsaBI GATNNNNATC 1 cut(s) 220
BsaHI GRCGYC 1 cut(s) 33
BsaJI CCNNGG 3 cut(s) 657, 843, 913
BsaXI ACNNNNNCTCC 2 cut(s) 1064, 1094
Bsc4I CCNNNNNNNGG 1 cut(s) 664
Bse118I RCCGGY 1 cut(s) 584
Bse1I ACTGG 3 cut(s) 400, 690, 706
Bse8I GATNNNNATC 1 cut(s) 220
BseBI CCWGG 1 cut(s) 1036
BseDI CCNNGG 3 cut(s) 657, 843, 913
BseGI GGATG 2 cut(s) 348, 811
BseJI GATNNNNATC 1 cut(s) 220
BseLI CCNNNNNNNGG 1 cut(s) 664
BseMII CTCAG 1 cut(s) 266
BseNI ACTGG 3 cut(s) 400, 690, 706
BseRI GAGGAG 6 cut(s) 275, 278, 720, 809, 812, 833
BseSI GKGCMC 1 cut(s) 571
BseXI GCAGC 2 cut(s) 139, 635
Bsh1285I CGRYCG 1 cut(s) 663
BshFI GGCC 1 cut(s) 420
BshNI GGYRCC 1 cut(s) 601
BsiEI CGRYCG 1 cut(s) 663
BsiHKCI CYCGRG 1 cut(s) 608
BsiSI CCGG 3 cut(s) 585, 659, 913
BslFI GGGAC 2 cut(s) 445, 1076
BslI CCNNNNNNNGG 1 cut(s) 664
BsmAI GTCTC 3 cut(s) 260, 467, 475
BsmFI GGGAC 2 cut(s) 445, 1076
BsnI GGCC 1 cut(s) 420
BsoBI CYCGRG 1 cut(s) 608
Bsp1286I GDGCHC 1 cut(s) 571
Bsp143I GATC 6 cut(s) 215, 283, 499, 780, 833, 949
Bsp19I CCATGG 1 cut(s) 843
BspACI CCGC 3 cut(s) 177, 352, 878
BspANI GGCC 1 cut(s) 420
BspCNI CTCAG 1 cut(s) 265
BspHI TCATGA 1 cut(s) 280
BspLI GGNNCC 2 cut(s) 603, 822
BspMAI CTGCAG 1 cut(s) 41
BspMI ACCTGC 1 cut(s) 53
BspQI GCTCTTC 1 cut(s) 306
BspT107I GGYRCC 1 cut(s) 601
BsrFI RCCGGY 1 cut(s) 584
BsrI ACTGG 3 cut(s) 400, 690, 706
BssAI RCCGGY 1 cut(s) 584
BssECI CCNNGG 3 cut(s) 657, 843, 913
BssMI GATC 6 cut(s) 215, 283, 499, 780, 833, 949
BssNI GRCGYC 1 cut(s) 33
BssT1I CCWWGG 1 cut(s) 843
Bst2UI CCWGG 1 cut(s) 1036
Bst4CI ACNGT 3 cut(s) 71, 358, 1076
Bst6I CTCTTC 2 cut(s) 276, 306
BstACI GRCGYC 1 cut(s) 33
BstAPI GCANNNNNTGC 1 cut(s) 45
BstC8I GCNNGC 1 cut(s) 642
BstDEI CTNAG 1 cut(s) 252
BstDSI CCRYGG 1 cut(s) 843
BstF5I GGATG 2 cut(s) 348, 811
BstHHI GCGC 2 cut(s) 443, 590
BstKTI GATC 6 cut(s) 218, 286, 502, 783, 836, 952
BstMAI GTCTC 3 cut(s) 260, 467, 475
BstMBI GATC 6 cut(s) 215, 283, 499, 780, 833, 949
BstMCI CGRYCG 1 cut(s) 663
BstMWI GCNNNNNNNGC 7 cut(s) 36, 45, 158, 373, 390, 447, 561
BstNI CCWGG 1 cut(s) 1036
BstNSI RCATGY 1 cut(s) 996
BstSCI CCNGG 3 cut(s) 657, 912, 1034
BstSFI CTRYAG 1 cut(s) 37
BstSLI GKGCMC 1 cut(s) 571
BstV1I GCAGC 2 cut(s) 139, 635
BstV2I GAAGAC 1 cut(s) 1023
BstX2I RGATCY 1 cut(s) 780
BstYI RGATCY 1 cut(s) 780
BsuRI GGCC 1 cut(s) 420
BtgI CCRYGG 1 cut(s) 843
BtsCI GGATG 2 cut(s) 348, 811
BtsI GCAGTG 1 cut(s) 1091
BtsIMutI CAGTG 1 cut(s) 1091
BveI ACCTGC 1 cut(s) 53
Cac8I GCNNGC 1 cut(s) 642
CaiI CAGNNNCTG 1 cut(s) 45
CciI TCATGA 1 cut(s) 280
CfoI GCGC 2 cut(s) 443, 590
Cfr10I RCCGGY 1 cut(s) 584
Cfr13I GGNCC 1 cut(s) 320
Csp6I GTAC 5 cut(s) 199, 346, 403, 693, 699
CspCI CAANNNNNGTGG 2 cut(s) 579, 614
CviAII CATG 6 cut(s) 281, 290, 317, 503, 844, 993
CviQI GTAC 5 cut(s) 199, 346, 403, 693, 699
DdeI CTNAG 1 cut(s) 252
DpnI GATC 6 cut(s) 217, 285, 501, 782, 835, 951
DpnII GATC 6 cut(s) 215, 283, 499, 780, 833, 949
Eam1104I CTCTTC 2 cut(s) 276, 306
EarI CTCTTC 2 cut(s) 276, 306
Eco130I CCWWGG 1 cut(s) 843
Eco32I GATATC 1 cut(s) 766
Eco47I GGWCC 1 cut(s) 320
Eco57I CTGAAG 1 cut(s) 768
Eco88I CYCGRG 1 cut(s) 608
EcoRII CCWGG 1 cut(s) 1034
EcoRV GATATC 1 cut(s) 766
EcoT14I CCWWGG 1 cut(s) 843
ErhI CCWWGG 1 cut(s) 843
FaeI CATG 6 cut(s) 284, 293, 320, 506, 847, 996
FaqI GGGAC 2 cut(s) 445, 1076
FatI CATG 6 cut(s) 280, 289, 316, 502, 843, 992
FauNDI CATATG 1 cut(s) 341
FbaI TGATCA 2 cut(s) 283, 499
Fnu4HI GCNGC 3 cut(s) 153, 178, 624
FokI GGATG 2 cut(s) 335, 818
Fsp4HI GCNGC 3 cut(s) 153, 178, 624
FspBI CTAG 2 cut(s) 671, 696
GlaI GCGC 2 cut(s) 442, 589
GluI GCNGC 3 cut(s) 153, 178, 624
GsuI CTGGAG 1 cut(s) 723
HaeIII GGCC 1 cut(s) 420
HapII CCGG 3 cut(s) 585, 659, 913
HhaI GCGC 2 cut(s) 443, 590
Hin1I GRCGYC 1 cut(s) 33
Hin1II CATG 6 cut(s) 284, 293, 320, 506, 847, 996
Hin6I GCGC 2 cut(s) 441, 588
HinP1I GCGC 2 cut(s) 441, 588
HincII GTYRAC 1 cut(s) 331
HindII GTYRAC 1 cut(s) 331
HindIII AAGCTT 1 cut(s) 1099
HinfI GANTC 5 cut(s) 268, 629, 686, 848, 1079
HpaII CCGG 3 cut(s) 585, 659, 913
HphI GGTGA 2 cut(s) 425, 695
Hpy166II GTNNAC 3 cut(s) 86, 331, 998
Hpy188I TCNGA 3 cut(s) 67, 954, 1023
Hpy188III TCNNGA 3 cut(s) 281, 671, 984
Hpy8I GTNNAC 3 cut(s) 86, 331, 998
Hpy99I CGWCG 4 cut(s) 35, 140, 148, 362
HpyAV CCTTC 2 cut(s) 136, 422
HpyCH4III ACNGT 3 cut(s) 71, 358, 1076
HpyCH4IV ACGT 1 cut(s) 33
HpyCH4V TGCA 7 cut(s) 39, 48, 155, 367, 623, 854, 1059
HpyF10VI GCNNNNNNNGC 7 cut(s) 36, 45, 158, 373, 390, 447, 561
HpyF3I CTNAG 1 cut(s) 252
HpySE526I ACGT 1 cut(s) 33
Hsp92I GRCGYC 1 cut(s) 33
Hsp92II CATG 6 cut(s) 284, 293, 320, 506, 847, 996
HspAI GCGC 2 cut(s) 441, 588
Ksp22I TGATCA 2 cut(s) 283, 499
Kzo9I GATC 6 cut(s) 215, 283, 499, 780, 833, 949
LguI GCTCTTC 1 cut(s) 306
LmnI GCTCC 2 cut(s) 389, 820
Lsp1109I GCAGC 2 cut(s) 139, 635
LweI GCATC 2 cut(s) 149, 863
MaeI CTAG 2 cut(s) 671, 696
MaeII ACGT 1 cut(s) 33
MaeIII GTNAC 1 cut(s) 1076
MalI GATC 6 cut(s) 217, 285, 501, 782, 835, 951
MboI GATC 6 cut(s) 215, 283, 499, 780, 833, 949
MflI RGATCY 1 cut(s) 780
MhlI GDGCHC 1 cut(s) 571
MluCI AATT 6 cut(s) 115, 550, 720, 924, 956, 1017
MlyI GAGTC 3 cut(s) 262, 695, 1073
MseI TTAA 3 cut(s) 8, 771, 789
MslI CAYNNNNRTG 2 cut(s) 279, 392
MspA1I CMGCKG 1 cut(s) 626
MspI CCGG 3 cut(s) 585, 659, 913
MspR9I CCNGG 3 cut(s) 659, 914, 1036
MvaI CCWGG 1 cut(s) 1036
MwoI GCNNNNNNNGC 7 cut(s) 36, 45, 158, 373, 390, 447, 561
NciI CCSGG 2 cut(s) 659, 914
NcoI CCATGG 1 cut(s) 843
NdeI CATATG 1 cut(s) 341
NdeII GATC 6 cut(s) 215, 283, 499, 780, 833, 949
NlaIII CATG 6 cut(s) 284, 293, 320, 506, 847, 996
NlaIV GGNNCC 2 cut(s) 603, 822
NmuCI GTSAC 1 cut(s) 1076
NspI RCATGY 1 cut(s) 996
PagI TCATGA 1 cut(s) 280
PciSI GCTCTTC 1 cut(s) 306
PfeI GAWTC 2 cut(s) 629, 848
PfoI TCCNGGA 1 cut(s) 1034
PkrI GCNGC 3 cut(s) 154, 179, 625
PleI GAGTC 3 cut(s) 262, 694, 1073
PpsI GAGTC 3 cut(s) 262, 694, 1073
PshBI ATTAAT 1 cut(s) 789
Psp6I CCWGG 1 cut(s) 1034
PspGI CCWGG 1 cut(s) 1034
PspN4I GGNNCC 2 cut(s) 603, 822
PspPI GGNCC 1 cut(s) 320
PstI CTGCAG 1 cut(s) 41
PstNI CAGNNNCTG 1 cut(s) 45
PsuI RGATCY 1 cut(s) 780
PvuII CAGCTG 1 cut(s) 626
RsaI GTAC 5 cut(s) 200, 347, 404, 694, 700
RsaNI GTAC 5 cut(s) 199, 346, 403, 693, 699
RseI CAYNNNNRTG 2 cut(s) 279, 392
SapI GCTCTTC 1 cut(s) 306
SaqAI TTAA 3 cut(s) 8, 771, 789
SatI GCNGC 3 cut(s) 153, 178, 624
Sau3AI GATC 6 cut(s) 215, 283, 499, 780, 833, 949
Sau96I GGNCC 1 cut(s) 320
ScaI AGTACT 3 cut(s) 200, 694, 700
SchI GAGTC 3 cut(s) 262, 695, 1073
ScrFI CCNGG 3 cut(s) 659, 914, 1036
SduI GDGCHC 1 cut(s) 571
SfaNI GCATC 2 cut(s) 149, 863
SfcI CTRYAG 1 cut(s) 37
SinI GGWCC 1 cut(s) 320
SmiMI CAYNNNNRTG 2 cut(s) 279, 392
SmlI CTYRAG 1 cut(s) 451
SmoI CTYRAG 1 cut(s) 451
SpeI ACTAGT 1 cut(s) 695
Sse9I AATT 6 cut(s) 115, 550, 720, 924, 956, 1017
SsiI CCGC 3 cut(s) 177, 352, 878
SspMI CTAG 2 cut(s) 671, 696
StyD4I CCNGG 3 cut(s) 657, 912, 1034
StyI CCWWGG 1 cut(s) 843
TaaI ACNGT 3 cut(s) 71, 358, 1076
TaiI ACGT 1 cut(s) 36
TaqI TCGA 2 cut(s) 426, 663
TaqII GACCGA 1 cut(s) 350
TasI AATT 6 cut(s) 115, 550, 720, 924, 956, 1017
TatI WGTACW 4 cut(s) 198, 402, 692, 698
TauI GCSGC 1 cut(s) 180
TfiI GAWTC 2 cut(s) 629, 848
Tru1I TTAA 3 cut(s) 8, 771, 789
Tru9I TTAA 3 cut(s) 8, 771, 789
TscAI CASTG 1 cut(s) 1098
TseFI GTSAC 1 cut(s) 1076
TseI GCWGC 2 cut(s) 152, 623
Tsp45I GTSAC 1 cut(s) 1076
TspDTI ATGAA 5 cut(s) 118, 263, 324, 500, 732
TspGWI ACGGA 1 cut(s) 1072
TspRI CASTG 1 cut(s) 1098
VpaK11BI GGWCC 1 cut(s) 320
VspI ATTAAT 1 cut(s) 789
XapI RAATTY 1 cut(s) 720
XbaI TCTAGA 1 cut(s) 670
XceI RCATGY 1 cut(s) 996
XspI CTAG 2 cut(s) 671, 696
ZraI GACGTC 1 cut(s) 34
ZrmI AGTACT 3 cut(s) 200, 694, 700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.