FvH4_2g23991

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0017156
Physical Location & Seq
Reverse (-)
19603837 .. 19604857
1021 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g23991.t1

Sequence Viewer

Length: 822 bp
ATGCAGCTCGGTCCTTCAACTTCACAAGATTTTGGAGTAATTCCAAATGAACAGCATCAAAATCATCATGCACCCCCGCCTCCACCTCCAGGTCCTCCTGTTAAGGTGAATCTCAAAGTCAATTGTTTGGAGTGTCAAGTGAAGCTGAAGAAGAAGTTGCAGAAGATAAGTGGGGTGAAAGATGTGGAGATAGTTCCAGAACAAGGTGGCACTGTAACAGTTTCAGGTAGCATAGATTCTAAGGTGCTCATGGAGGTGATCCGAATTAATTACAAAAAGGCCGTGCTAGTTTGCCCAGTTGATAAGAATGAACCCTTTTGCTCCAAAAATCAAAGCAAAAAATGTTCTTGCTCTGATGATGAACATGATGATAGCAAGGAAGACGATCATCACCACAAAGTTATTGCTGCAAAAAACAATCGTGACAATGATGCCGGTCTAATAAGTGAACCCAAGAATGAGAAGAAGAGAAGGATGTGTGGTATGATGAGATGTTTTGGCAAGGCAAGTTGCCCTAGTACGGCCATCCGTCCAGCTCCTCGACCGGTGCAATCACCATCACCGGTGCCTCGGCCCCTCATGGGATTTCAGGCCAATGGGTATTTCCCCGGACCGCCAAGGCCAGGATTTCGGCCAGGGATGCTGGGGATGCCATGGATGCCGCCACATGGGGCACCGCCGCCGTATGGGTATTCAGGGAGGCCGCCATTATTACCTATGGGCTCCGGGTATCGTTCTTTCATGATGAGGTCGCCGCCGAGGTCGAACCCTATGGTACATTACACCAATTATACGGACAATTTTCGTTATCCTTATAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

274

Amino Acids

30.26

Weight (kDa)

9.42

Isoelectric Point (pI)

58.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017156)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04900
fragaria_vesca FvH4_2g23991
malus_domestica MD11G1290300.v1.1
prunus_persica Prupe.7G246000_v2.0.a1 Prupe.8G245300_v2.0.a1
rosa_laevigata RLG00000012095
rosa_multiflora Rmu_sc0002226.1_g000022
rosa_roxburghii Rroxscaffold_7G00175820
rosa_rugosa Rorug06G0221100
rosa_samantha Rh6AG333700 Rh6BG340700 Rh6CG346900 Rh6DG334100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 816
AccB1I GGYRCC 2 cut(s) 565, 673
AciI CCGC 7 cut(s) 77, 614, 662, 677, 680, 704, 755
AclWI GGATC 1 cut(s) 253
AcoI YGGCCR 2 cut(s) 522, 632
AcuI CTGAAG 1 cut(s) 167
AfaI GTAC 2 cut(s) 520, 777
AfiI CCNNNNNNNGG 7 cut(s) 89, 203, 520, 581, 623, 668, 686
AgeI ACCGGT 2 cut(s) 544, 562
AgsI TTSAA 1 cut(s) 18
AjnI CCWGG 3 cut(s) 88, 622, 634
AjuI GAANNNNNNNTTGG 2 cut(s) 587, 619
AluBI AGCT 3 cut(s) 7, 145, 536
AluI AGCT 3 cut(s) 7, 145, 536
Alw21I GWGCWC 1 cut(s) 249
AlwI GGATC 1 cut(s) 253
AoxI GGCC 7 cut(s) 279, 522, 572, 591, 620, 632, 701
ApeKI GCWGC 2 cut(s) 4, 407
AseI ATTAAT 1 cut(s) 267
AsiGI ACCGGT 2 cut(s) 544, 562
AspS9I GGNCC 4 cut(s) 11, 92, 573, 611
AsuC2I CCSGG 2 cut(s) 609, 727
AsuHPI GGTGA 6 cut(s) 118, 187, 268, 383, 546, 552
AvaII GGWCC 3 cut(s) 11, 92, 611
BaeGI GKGCMC 1 cut(s) 676
BanI GGYRCC 2 cut(s) 565, 673
BanII GRGCYC 1 cut(s) 725
BbsI GAAGAC 1 cut(s) 387
Bbv12I GWGCWC 1 cut(s) 249
BbvI GCAGC 2 cut(s) 16, 394
BccI CCATC 2 cut(s) 533, 565
BceAI ACGGC 3 cut(s) 266, 537, 667
BciT130I CCWGG 3 cut(s) 90, 624, 636
BcnI CCSGG 2 cut(s) 609, 727
BfaI CTAG 2 cut(s) 287, 516
BisI GCNGC 6 cut(s) 5, 408, 662, 680, 704, 755
BlsI GCNGC 6 cut(s) 6, 409, 663, 681, 705, 756
Bme1390I CCNGG 5 cut(s) 90, 609, 624, 636, 727
Bme18I GGWCC 3 cut(s) 11, 92, 611
BmgT120I GGNCC 4 cut(s) 11, 92, 573, 611
BmiI GGNNCC 4 cut(s) 567, 575, 675, 724
BmrFI CCNGG 5 cut(s) 90, 609, 624, 636, 727
BmrI ACTGGG 1 cut(s) 290
BmsI GCATC 5 cut(s) 64, 421, 630, 639, 648
BmuI ACTGGG 1 cut(s) 290
BpiI GAAGAC 1 cut(s) 387
BpmI CTGGAG 1 cut(s) 72
BpuMI CCSGG 2 cut(s) 609, 727
BsaJI CCNNGG 6 cut(s) 569, 607, 617, 635, 653, 758
BsaWI WCCGGW 2 cut(s) 544, 562
Bsc4I CCNNNNNNNGG 7 cut(s) 89, 203, 520, 581, 623, 668, 686
Bse118I RCCGGY 3 cut(s) 434, 544, 562
Bse1I ACTGG 1 cut(s) 296
BseBI CCWGG 3 cut(s) 90, 624, 636
BseDI CCNNGG 6 cut(s) 569, 607, 617, 635, 653, 758
BseGI GGATG 5 cut(s) 480, 525, 645, 654, 663
BseLI CCNNNNNNNGG 7 cut(s) 89, 203, 520, 581, 623, 668, 686
BseNI ACTGG 1 cut(s) 296
BseRI GAGGAG 1 cut(s) 528
BseSI GKGCMC 1 cut(s) 676
BseXI GCAGC 2 cut(s) 16, 394
BseYI CCCAGC 1 cut(s) 643
Bsh1285I CGRYCG 1 cut(s) 545
BshFI GGCC 7 cut(s) 281, 524, 574, 593, 622, 634, 703
BshNI GGYRCC 2 cut(s) 565, 673
BshTI ACCGGT 2 cut(s) 544, 562
BsiEI CGRYCG 1 cut(s) 545
BsiHKAI GWGCWC 1 cut(s) 249
BsiSI CCGG 5 cut(s) 435, 545, 563, 609, 726
BslI CCNNNNNNNGG 7 cut(s) 89, 203, 520, 581, 623, 668, 686
BsnI GGCC 7 cut(s) 281, 524, 574, 593, 622, 634, 703
Bsp1286I GDGCHC 3 cut(s) 249, 676, 725
Bsp143I GATC 2 cut(s) 258, 385
Bsp19I CCATGG 1 cut(s) 653
BspACI CCGC 7 cut(s) 77, 614, 662, 677, 680, 704, 755
BspANI GGCC 7 cut(s) 281, 524, 574, 593, 622, 634, 703
BspHI TCATGA 1 cut(s) 741
BspLI GGNNCC 4 cut(s) 567, 575, 675, 724
BspPI GGATC 1 cut(s) 253
BspT107I GGYRCC 2 cut(s) 565, 673
BsrFI RCCGGY 3 cut(s) 434, 544, 562
BsrI ACTGG 1 cut(s) 296
BssAI RCCGGY 3 cut(s) 434, 544, 562
BssECI CCNNGG 6 cut(s) 569, 607, 617, 635, 653, 758
BssMI GATC 2 cut(s) 258, 385
BssT1I CCWWGG 2 cut(s) 617, 653
Bst2UI CCWGG 3 cut(s) 90, 624, 636
Bst4CI ACNGT 2 cut(s) 214, 220
Bst6I CTCTTC 1 cut(s) 461
BstDEI CTNAG 1 cut(s) 240
BstDSI CCRYGG 1 cut(s) 653
BstF5I GGATG 5 cut(s) 480, 525, 645, 654, 663
BstKTI GATC 2 cut(s) 261, 388
BstMBI GATC 2 cut(s) 258, 385
BstMCI CGRYCG 1 cut(s) 545
BstMWI GCNNNNNNNGC 3 cut(s) 640, 649, 658
BstNI CCWGG 3 cut(s) 90, 624, 636
BstSCI CCNGG 5 cut(s) 88, 607, 622, 634, 725
BstSLI GKGCMC 1 cut(s) 676
BstV1I GCAGC 2 cut(s) 16, 394
BstV2I GAAGAC 1 cut(s) 387
BsuRI GGCC 7 cut(s) 281, 524, 574, 593, 622, 634, 703
BtgI CCRYGG 1 cut(s) 653
BtsCI GGATG 5 cut(s) 480, 525, 645, 654, 663
BtsIMutI CAGTG 1 cut(s) 210
CciI TCATGA 1 cut(s) 741
Cfr10I RCCGGY 3 cut(s) 434, 544, 562
Cfr13I GGNCC 4 cut(s) 11, 92, 573, 611
CpoI CGGWCCG 1 cut(s) 611
Csp6I GTAC 2 cut(s) 519, 776
CspAI ACCGGT 2 cut(s) 544, 562
CspI CGGWCCG 1 cut(s) 611
CviAII CATG 7 cut(s) 68, 250, 365, 580, 654, 668, 742
CviQI GTAC 2 cut(s) 519, 776
DdeI CTNAG 1 cut(s) 240
DpnI GATC 2 cut(s) 260, 387
DpnII GATC 2 cut(s) 258, 385
EaeI YGGCCR 2 cut(s) 522, 632
Eam1104I CTCTTC 1 cut(s) 461
EarI CTCTTC 1 cut(s) 461
Eco130I CCWWGG 2 cut(s) 617, 653
Eco24I GRGCYC 1 cut(s) 725
Eco47I GGWCC 3 cut(s) 11, 92, 611
Eco57I CTGAAG 1 cut(s) 167
EcoO109I RGGNCCY 1 cut(s) 92
EcoRII CCWGG 3 cut(s) 88, 622, 634
EcoT14I CCWWGG 2 cut(s) 617, 653
EcoT38I GRGCYC 1 cut(s) 725
ErhI CCWWGG 2 cut(s) 617, 653
FaeI CATG 7 cut(s) 71, 253, 368, 583, 657, 671, 745
FatI CATG 7 cut(s) 67, 249, 364, 579, 653, 667, 741
FauI CCCGC 1 cut(s) 84
Fnu4HI GCNGC 6 cut(s) 5, 408, 662, 680, 704, 755
FokI GGATG 5 cut(s) 487, 512, 652, 661, 670
FriOI GRGCYC 1 cut(s) 725
Fsp4HI GCNGC 6 cut(s) 5, 408, 662, 680, 704, 755
FspBI CTAG 2 cut(s) 287, 516
GluI GCNGC 6 cut(s) 5, 408, 662, 680, 704, 755
GsaI CCCAGC 1 cut(s) 647
GsuI CTGGAG 1 cut(s) 72
HaeIII GGCC 7 cut(s) 281, 524, 574, 593, 622, 634, 703
HapII CCGG 5 cut(s) 435, 545, 563, 609, 726
Hin1II CATG 7 cut(s) 71, 253, 368, 583, 657, 671, 745
HinfI GANTC 2 cut(s) 109, 236
HpaII CCGG 5 cut(s) 435, 545, 563, 609, 726
HphI GGTGA 6 cut(s) 118, 187, 268, 383, 546, 552
Hpy166II GTNNAC 1 cut(s) 449
Hpy188I TCNGA 2 cut(s) 263, 355
Hpy188III TCNNGA 3 cut(s) 197, 422, 742
Hpy8I GTNNAC 1 cut(s) 449
HpyAV CCTTC 2 cut(s) 24, 465
HpyCH4III ACNGT 2 cut(s) 214, 220
HpyCH4V TGCA 5 cut(s) 4, 71, 160, 410, 550
HpyF10VI GCNNNNNNNGC 3 cut(s) 640, 649, 658
HpyF3I CTNAG 1 cut(s) 240
Hsp92II CATG 7 cut(s) 71, 253, 368, 583, 657, 671, 745
Kzo9I GATC 2 cut(s) 258, 385
LmnI GCTCC 3 cut(s) 326, 541, 728
Lsp1109I GCAGC 2 cut(s) 16, 394
LweI GCATC 5 cut(s) 64, 421, 630, 639, 648
MaeI CTAG 2 cut(s) 287, 516
MaeIII GTNAC 2 cut(s) 214, 422
MalI GATC 2 cut(s) 260, 387
MboI GATC 2 cut(s) 258, 385
MboII GAAGA 6 cut(s) 160, 163, 175, 392, 475, 478
MfeI CAATTG 1 cut(s) 121
MhlI GDGCHC 3 cut(s) 249, 676, 725
MluCI AATT 7 cut(s) 39, 121, 264, 268, 787, 799, 817
MseI TTAA 2 cut(s) 102, 267
MslI CAYNNNNRTG 1 cut(s) 254
MspI CCGG 5 cut(s) 435, 545, 563, 609, 726
MspR9I CCNGG 5 cut(s) 90, 609, 624, 636, 727
MunI CAATTG 1 cut(s) 121
MvaI CCWGG 3 cut(s) 90, 624, 636
MwoI GCNNNNNNNGC 3 cut(s) 640, 649, 658
NciI CCSGG 2 cut(s) 609, 727
NcoI CCATGG 1 cut(s) 653
NdeII GATC 2 cut(s) 258, 385
NlaIII CATG 7 cut(s) 71, 253, 368, 583, 657, 671, 745
NlaIV GGNNCC 4 cut(s) 567, 575, 675, 724
NmeAIII GCCGAG 2 cut(s) 550, 783
NmuCI GTSAC 1 cut(s) 422
PagI TCATGA 1 cut(s) 741
PfeI GAWTC 2 cut(s) 109, 236
PinAI ACCGGT 2 cut(s) 544, 562
PkrI GCNGC 6 cut(s) 6, 409, 663, 681, 705, 756
PpuMI RGGWCCY 1 cut(s) 92
PshBI ATTAAT 1 cut(s) 267
PsiI TTATAA 1 cut(s) 816
Psp5II RGGWCCY 1 cut(s) 92
Psp6I CCWGG 3 cut(s) 88, 622, 634
PspFI CCCAGC 1 cut(s) 643
PspGI CCWGG 3 cut(s) 88, 622, 634
PspN4I GGNNCC 4 cut(s) 567, 575, 675, 724
PspPI GGNCC 4 cut(s) 11, 92, 573, 611
PspPPI RGGWCCY 1 cut(s) 92
RsaI GTAC 2 cut(s) 520, 777
RsaNI GTAC 2 cut(s) 519, 776
RseI CAYNNNNRTG 1 cut(s) 254
Rsr2I CGGWCCG 1 cut(s) 611
RsrII CGGWCCG 1 cut(s) 611
SaqAI TTAA 2 cut(s) 102, 267
SatI GCNGC 6 cut(s) 5, 408, 662, 680, 704, 755
Sau3AI GATC 2 cut(s) 258, 385
Sau96I GGNCC 4 cut(s) 11, 92, 573, 611
ScrFI CCNGG 5 cut(s) 90, 609, 624, 636, 727
SduI GDGCHC 3 cut(s) 249, 676, 725
SfaNI GCATC 5 cut(s) 64, 421, 630, 639, 648
SgrAI CRCCGGYG 1 cut(s) 562
SinI GGWCC 3 cut(s) 11, 92, 611
SmiMI CAYNNNNRTG 1 cut(s) 254
Sse9I AATT 7 cut(s) 39, 121, 264, 268, 787, 799, 817
SsiI CCGC 7 cut(s) 77, 614, 662, 677, 680, 704, 755
SspMI CTAG 2 cut(s) 287, 516
StyD4I CCNGG 5 cut(s) 88, 607, 622, 634, 725
StyI CCWWGG 2 cut(s) 617, 653
TaaI ACNGT 2 cut(s) 214, 220
TaqI TCGA 2 cut(s) 541, 764
TasI AATT 7 cut(s) 39, 121, 264, 268, 787, 799, 817
TauI GCSGC 4 cut(s) 664, 682, 706, 757
TfiI GAWTC 2 cut(s) 109, 236
Tru1I TTAA 2 cut(s) 102, 267
Tru9I TTAA 2 cut(s) 102, 267
TscAI CASTG 1 cut(s) 217
TseFI GTSAC 1 cut(s) 422
TseI GCWGC 2 cut(s) 4, 407
Tsp45I GTSAC 1 cut(s) 422
TspDTI ATGAA 4 cut(s) 63, 324, 375, 730
TspGWI ACGGA 2 cut(s) 518, 809
TspRI CASTG 1 cut(s) 217
VpaK11BI GGWCC 3 cut(s) 11, 92, 611
VspI ATTAAT 1 cut(s) 267
XspI CTAG 2 cut(s) 287, 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.