Rh6AG333700

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0017156
Physical Location & Seq
Reverse (-)
53474593 .. 53475581
989 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG333700.1

Sequence Viewer

Length: 801 bp
ATGGATTCTACAAAAGCATTATATTTGCAGCCCTATTCTTCTGCATCACAAGGATTTGAAATAATTCCAAAGGAACAGCATCAGCATCATCATGCACCCCCGCCTCGACCTCCAGGTCCTCCTATTAAAGTGAATCTCAAAAGTAATTGTTTGAATTGTCAAGTGAAGCTGAAGAAAAAGTTGCAGAAGATGAGTGGGGTGAATGATGTGGACATAGTTCCAGAACAAGGTGGCACTGTAACAGTTTCAGGTAGCATAGACTCTAAGGTGCTCATGGAGGTGATCCGAAATAATTACAAAAAGGCCAAGTTACTTTGCCCCTTTGATCAGAAGGAGCCCTCCGAAAATACAGTTGGAAAAAAATTTTCGTGCTCTGATGATGAACATGATGATAGCGAGGACGGCAATGACCACAATTTTGCTGTAAACAATAGTAACAACGAGGCCGGTCTAACTGAACCGAAGAAAGAGAAGAAGACGAAGATGTGTGGTATAATGAGATGTTTTGGCAAGAAAAGTAACCCTATGGCCATGCGTCCACCTCCTCCCGCACCATCACCTGAGCCTCGGCCCCCCATGGGTTTTCCGACAAATGGATTTTTCCTTGGGCCGCCAAGGCCCGGATTTCGGCCGGGAATGCCATGGATGCCGCCAATTGGGCCACCACCGCCATATGGGTATTTAGGGAGGCCGCCATTACCCACGGACTACGGATATCCTTTCCCGAGGTCGCCGCCAAGATTGAACCCTATGGTACACTACACCAGTTATAAGGACAATTATCGTCATCCATACCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

266

Amino Acids

29.7

Weight (kDa)

9.24

Isoelectric Point (pI)

55.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017156)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04900
fragaria_vesca FvH4_2g23991
malus_domestica MD11G1290300.v1.1
prunus_persica Prupe.7G246000_v2.0.a1 Prupe.8G245300_v2.0.a1
rosa_laevigata RLG00000012095
rosa_multiflora Rmu_sc0002226.1_g000022
rosa_roxburghii Rroxscaffold_7G00175820
rosa_rugosa Rorug06G0221100
rosa_samantha Rh6AG333700 Rh6BG340700 Rh6CG346900 Rh6DG334100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 771
AasI GACNNNNNNGTC 1 cut(s) 114
AciI CCGC 7 cut(s) 101, 549, 611, 650, 668, 692, 734
AclWI GGATC 1 cut(s) 277
AcoI YGGCCR 2 cut(s) 528, 629
AcsI RAATTY 1 cut(s) 362
AcuI CTGAAG 1 cut(s) 191
AfaI GTAC 1 cut(s) 756
AfiI CCNNNNNNNGG 7 cut(s) 227, 578, 593, 620, 627, 656, 674
AgsI TTSAA 3 cut(s) 59, 154, 745
AjnI CCWGG 1 cut(s) 112
AjuI GAANNNNNNNTTGG 2 cut(s) 336, 368
AluBI AGCT 1 cut(s) 169
AluI AGCT 1 cut(s) 169
Alw21I GWGCWC 2 cut(s) 273, 374
AlwI GGATC 1 cut(s) 277
Ama87I CYCGRG 1 cut(s) 724
AoxI GGCC 9 cut(s) 303, 444, 528, 569, 608, 617, 629, 659, 689
ApeKI GCWGC 1 cut(s) 28
ApoI RAATTY 1 cut(s) 362
ArsI GACNNNNNNTTYG 2 cut(s) 401, 433
Asp700I GAANNNNTTC 1 cut(s) 63
AspS9I GGNCC 5 cut(s) 116, 570, 608, 618, 659
AsuC2I CCSGG 2 cut(s) 621, 633
AsuHPI GGTGA 3 cut(s) 211, 292, 549
AvaI CYCGRG 1 cut(s) 724
AvaII GGWCC 1 cut(s) 116
BalI TGGCCA 1 cut(s) 530
BanII GRGCYC 1 cut(s) 339
BbsI GAAGAC 1 cut(s) 482
Bbv12I GWGCWC 2 cut(s) 273, 374
BbvI GCAGC 1 cut(s) 40
BccI CCATC 1 cut(s) 562
BceAI ACGGC 1 cut(s) 418
BciT130I CCWGG 1 cut(s) 114
BclI TGATCA 1 cut(s) 325
BcnI CCSGG 2 cut(s) 621, 633
BglI GCCNNNNNGGC 2 cut(s) 616, 658
BisI GCNGC 5 cut(s) 29, 611, 650, 692, 734
BlsI GCNGC 5 cut(s) 30, 612, 651, 693, 735
Bme1390I CCNGG 3 cut(s) 114, 621, 633
Bme18I GGWCC 1 cut(s) 116
BmeT110I CYCGRG 1 cut(s) 724
BmgT120I GGNCC 5 cut(s) 116, 570, 608, 618, 659
BmiI GGNNCC 2 cut(s) 336, 572
BmrFI CCNGG 3 cut(s) 114, 621, 633
BmsI GCATC 4 cut(s) 53, 88, 94, 636
BpiI GAAGAC 1 cut(s) 482
BpmI CTGGAG 1 cut(s) 96
Bpu10I CCTNAGC 1 cut(s) 561
BpuMI CCSGG 2 cut(s) 621, 633
BsaJI CCNNGG 7 cut(s) 566, 576, 604, 614, 641, 702, 725
Bsc4I CCNNNNNNNGG 7 cut(s) 227, 578, 593, 620, 627, 656, 674
Bse118I RCCGGY 1 cut(s) 446
Bse1I ACTGG 2 cut(s) 765, 796
Bse3DI GCAATG 1 cut(s) 412
BseBI CCWGG 1 cut(s) 114
BseDI CCNNGG 7 cut(s) 566, 576, 604, 614, 641, 702, 725
BseGI GGATG 2 cut(s) 651, 787
BseLI CCNNNNNNNGG 7 cut(s) 227, 578, 593, 620, 627, 656, 674
BseMI GCAATG 1 cut(s) 412
BseMII CTCAG 1 cut(s) 552
BseNI ACTGG 2 cut(s) 765, 796
BseRI GAGGAG 1 cut(s) 534
BseX3I CGGCCG 1 cut(s) 629
BseXI GCAGC 1 cut(s) 40
Bsh1285I CGRYCG 1 cut(s) 632
BshFI GGCC 9 cut(s) 305, 446, 530, 571, 610, 619, 631, 661, 691
BsiEI CGRYCG 1 cut(s) 632
BsiHKAI GWGCWC 2 cut(s) 273, 374
BsiHKCI CYCGRG 1 cut(s) 724
BsiSI CCGG 3 cut(s) 447, 621, 632
BslI CCNNNNNNNGG 7 cut(s) 227, 578, 593, 620, 627, 656, 674
BsmI GAATGC 1 cut(s) 642
BsnI GGCC 9 cut(s) 305, 446, 530, 571, 610, 619, 631, 661, 691
BsoBI CYCGRG 1 cut(s) 724
Bsp1286I GDGCHC 3 cut(s) 273, 339, 374
Bsp143I GATC 2 cut(s) 282, 325
Bsp19I CCATGG 2 cut(s) 576, 641
BspACI CCGC 7 cut(s) 101, 549, 611, 650, 668, 692, 734
BspANI GGCC 9 cut(s) 305, 446, 530, 571, 610, 619, 631, 661, 691
BspCNI CTCAG 1 cut(s) 553
BspLI GGNNCC 2 cut(s) 336, 572
BspPI GGATC 1 cut(s) 277
BsrDI GCAATG 1 cut(s) 412
BsrFI RCCGGY 1 cut(s) 446
BsrI ACTGG 2 cut(s) 765, 796
BssAI RCCGGY 1 cut(s) 446
BssECI CCNNGG 7 cut(s) 566, 576, 604, 614, 641, 702, 725
BssMI GATC 2 cut(s) 282, 325
BssT1I CCWWGG 4 cut(s) 576, 604, 614, 641
Bst2UI CCWGG 1 cut(s) 114
Bst4CI ACNGT 3 cut(s) 238, 244, 352
BstDEI CTNAG 2 cut(s) 264, 561
BstDSI CCRYGG 3 cut(s) 576, 641, 702
BstF5I GGATG 2 cut(s) 651, 787
BstKTI GATC 2 cut(s) 285, 328
BstMBI GATC 2 cut(s) 282, 325
BstMCI CGRYCG 1 cut(s) 632
BstMWI GCNNNNNNNGC 6 cut(s) 402, 616, 637, 646, 658, 667
BstNI CCWGG 1 cut(s) 114
BstSCI CCNGG 3 cut(s) 112, 619, 631
BstV1I GCAGC 1 cut(s) 40
BstV2I GAAGAC 1 cut(s) 482
BstZI CGGCCG 1 cut(s) 629
BsuRI GGCC 9 cut(s) 305, 446, 530, 571, 610, 619, 631, 661, 691
BtgI CCRYGG 3 cut(s) 576, 641, 702
BtsCI GGATG 2 cut(s) 651, 787
BtsIMutI CAGTG 1 cut(s) 234
Cfr10I RCCGGY 1 cut(s) 446
Cfr13I GGNCC 5 cut(s) 116, 570, 608, 618, 659
CseI GACGC 1 cut(s) 524
Csp6I GTAC 1 cut(s) 755
CviAII CATG 6 cut(s) 92, 274, 386, 532, 577, 642
CviQI GTAC 1 cut(s) 755
DdeI CTNAG 2 cut(s) 264, 561
DpnI GATC 2 cut(s) 284, 327
DpnII GATC 2 cut(s) 282, 325
DrdI GACNNNNNNGTC 1 cut(s) 114
DseDI GACNNNNNNGTC 1 cut(s) 114
EaeI YGGCCR 2 cut(s) 528, 629
EagI CGGCCG 1 cut(s) 629
EclXI CGGCCG 1 cut(s) 629
Eco130I CCWWGG 4 cut(s) 576, 604, 614, 641
Eco24I GRGCYC 1 cut(s) 339
Eco32I GATATC 1 cut(s) 716
Eco47I GGWCC 1 cut(s) 116
Eco52I CGGCCG 1 cut(s) 629
Eco57I CTGAAG 1 cut(s) 191
Eco88I CYCGRG 1 cut(s) 724
EcoO109I RGGNCCY 1 cut(s) 116
EcoRII CCWGG 1 cut(s) 112
EcoRV GATATC 1 cut(s) 716
EcoT14I CCWWGG 4 cut(s) 576, 604, 614, 641
EcoT38I GRGCYC 1 cut(s) 339
ErhI CCWWGG 4 cut(s) 576, 604, 614, 641
FaeI CATG 6 cut(s) 95, 277, 389, 535, 580, 645
FatI CATG 6 cut(s) 91, 273, 385, 531, 576, 641
FauI CCCGC 2 cut(s) 108, 556
FauNDI CATATG 1 cut(s) 673
FbaI TGATCA 1 cut(s) 325
Fnu4HI GCNGC 5 cut(s) 29, 611, 650, 692, 734
FokI GGATG 2 cut(s) 658, 774
FriOI GRGCYC 1 cut(s) 339
Fsp4HI GCNGC 5 cut(s) 29, 611, 650, 692, 734
GluI GCNGC 5 cut(s) 29, 611, 650, 692, 734
GsuI CTGGAG 1 cut(s) 96
HaeIII GGCC 9 cut(s) 305, 446, 530, 571, 610, 619, 631, 661, 691
HapII CCGG 3 cut(s) 447, 621, 632
HgaI GACGC 1 cut(s) 524
Hin1II CATG 6 cut(s) 95, 277, 389, 535, 580, 645
HinfI GANTC 3 cut(s) 5, 133, 260
HpaII CCGG 3 cut(s) 447, 621, 632
HphI GGTGA 3 cut(s) 211, 292, 549
Hpy166II GTNNAC 4 cut(s) 211, 427, 539, 757
Hpy188I TCNGA 5 cut(s) 287, 330, 343, 376, 588
Hpy188III TCNNGA 2 cut(s) 221, 724
Hpy8I GTNNAC 4 cut(s) 211, 427, 539, 757
HpyAV CCTTC 1 cut(s) 325
HpyCH4III ACNGT 3 cut(s) 238, 244, 352
HpyCH4V TGCA 4 cut(s) 28, 44, 95, 184
HpyF10VI GCNNNNNNNGC 6 cut(s) 402, 616, 637, 646, 658, 667
HpyF3I CTNAG 2 cut(s) 264, 561
Hsp92II CATG 6 cut(s) 95, 277, 389, 535, 580, 645
Ksp22I TGATCA 1 cut(s) 325
Kzo9I GATC 2 cut(s) 282, 325
LmnI GCTCC 1 cut(s) 334
LpnPI CCDG 9 cut(s) 99, 126, 234, 234, 460, 573, 634, 645, 778
Lsp1109I GCAGC 1 cut(s) 40
LweI GCATC 4 cut(s) 53, 88, 94, 636
MaeIII GTNAC 4 cut(s) 238, 309, 434, 518
MalI GATC 2 cut(s) 284, 327
MboI GATC 2 cut(s) 282, 325
MboII GAAGA 7 cut(s) 30, 184, 199, 475, 484, 487, 493
MfeI CAATTG 1 cut(s) 654
MhlI GDGCHC 3 cut(s) 273, 339, 374
MlsI TGGCCA 1 cut(s) 530
MluCI AATT 8 cut(s) 63, 145, 154, 292, 362, 415, 654, 778
MluNI TGGCCA 1 cut(s) 530
MlyI GAGTC 1 cut(s) 254
MmeI TCCRAC 2 cut(s) 334, 611
Mox20I TGGCCA 1 cut(s) 530
MroXI GAANNNNTTC 1 cut(s) 63
MscI TGGCCA 1 cut(s) 530
MseI TTAA 1 cut(s) 126
MslI CAYNNNNRTG 3 cut(s) 90, 278, 796
Msp20I TGGCCA 1 cut(s) 530
MspI CCGG 3 cut(s) 447, 621, 632
MspR9I CCNGG 3 cut(s) 114, 621, 633
MunI CAATTG 1 cut(s) 654
Mva1269I GAATGC 1 cut(s) 642
MvaI CCWGG 1 cut(s) 114
MwoI GCNNNNNNNGC 6 cut(s) 402, 616, 637, 646, 658, 667
NciI CCSGG 2 cut(s) 621, 633
NcoI CCATGG 2 cut(s) 576, 641
NdeI CATATG 1 cut(s) 673
NdeII GATC 2 cut(s) 282, 325
NlaIII CATG 6 cut(s) 95, 277, 389, 535, 580, 645
NlaIV GGNNCC 2 cut(s) 336, 572
NmeAIII GCCGAG 1 cut(s) 547
PctI GAATGC 1 cut(s) 642
PdmI GAANNNNTTC 1 cut(s) 63
PfeI GAWTC 2 cut(s) 5, 133
PkrI GCNGC 5 cut(s) 30, 612, 651, 693, 735
PleI GAGTC 1 cut(s) 254
PpsI GAGTC 1 cut(s) 254
PpuMI RGGWCCY 1 cut(s) 116
PsiI TTATAA 1 cut(s) 771
Psp5II RGGWCCY 1 cut(s) 116
Psp6I CCWGG 1 cut(s) 112
PspGI CCWGG 1 cut(s) 112
PspN4I GGNNCC 2 cut(s) 336, 572
PspPI GGNCC 5 cut(s) 116, 570, 608, 618, 659
PspPPI RGGWCCY 1 cut(s) 116
RsaI GTAC 1 cut(s) 756
RsaNI GTAC 1 cut(s) 755
RseI CAYNNNNRTG 3 cut(s) 90, 278, 796
SaqAI TTAA 1 cut(s) 126
SatI GCNGC 5 cut(s) 29, 611, 650, 692, 734
Sau3AI GATC 2 cut(s) 282, 325
Sau96I GGNCC 5 cut(s) 116, 570, 608, 618, 659
SchI GAGTC 1 cut(s) 254
ScrFI CCNGG 3 cut(s) 114, 621, 633
SduI GDGCHC 3 cut(s) 273, 339, 374
SfaNI GCATC 4 cut(s) 53, 88, 94, 636
SfiI GGCCNNNNNGGCC 1 cut(s) 616
SinI GGWCC 1 cut(s) 116
SmiMI CAYNNNNRTG 3 cut(s) 90, 278, 796
Sse9I AATT 8 cut(s) 63, 145, 154, 292, 362, 415, 654, 778
SsiI CCGC 7 cut(s) 101, 549, 611, 650, 668, 692, 734
StyD4I CCNGG 3 cut(s) 112, 619, 631
StyI CCWWGG 4 cut(s) 576, 604, 614, 641
TaaI ACNGT 3 cut(s) 238, 244, 352
TaqI TCGA 1 cut(s) 106
TasI AATT 8 cut(s) 63, 145, 154, 292, 362, 415, 654, 778
TauI GCSGC 4 cut(s) 613, 652, 694, 736
TfiI GAWTC 2 cut(s) 5, 133
Tru1I TTAA 1 cut(s) 126
Tru9I TTAA 1 cut(s) 126
TscAI CASTG 1 cut(s) 241
TseI GCWGC 1 cut(s) 28
TspDTI ATGAA 1 cut(s) 396
TspGWI ACGGA 2 cut(s) 719, 726
TspRI CASTG 1 cut(s) 241
VpaK11BI GGWCC 1 cut(s) 116
XapI RAATTY 1 cut(s) 362
XmnI GAANNNNTTC 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.